Rw1G035050

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000663
Physical Location & Seq
Forward (+)
62907832 .. 62910251
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035050.1

Sequence Viewer

Length: 1437 bp
ATGTGTACCAATTTCCTTCTGTTAAGGCTGTTGATAGCAGCAGGGTTGTTGGCAATGGCAACCGCCGCAAAAGTGACTCCACATAACTACGCAGATGCTCTGTCGAAGTCCACTTTATTTTTTGAAGGCCAGAGATCTGGCAAGTTACCGAATTCTCAACGAATGAATTGGAGGAAACATTTGCCCTACATGATGGCAGCGATGTTCATGGATCTGGTGGGGGGTTACCATGATGCTGGTGACAATGTGAAGTTCAACTTTCCCATGGCCGTCACAACAACATTGTTGTCATGGAGCGTTCTAGAGTTTGGAGATTTGATGGGATCTGAACTTCAACATGCTTTGGAAGCTATAAGATGGGCAACTGATTATTTTCTTAAAGCCACAAATGATCCTGGTTCAGTAGTTGGCGTAGCTGAAGACGTGGATACCCCAAGAACCACATATGTGGTAACTAAAGAAAAACCAGGTTCAGAAGTTTCTGCGGAGATTGCAGCCATGCTTGCAGCTTCCTCTATAGTGTTTAACCAATCATACAAAGCCTACTCTGACTCGCTTCTTAAGCGAGCCATCCAAGTGTTTCAATTTGCAGATCAGTATCAGGGATCTTACAATACTAGTATTGGAGACGGAGCCTGCCCATTTTACTGCGATTTTAGTGGCTACATGCTATACAAGGCAACAAAGGAACCAAATTATTGGAAATATGTCAAAGACCACATACACAATCTGGAGTCGTCAAACATTGTAGTGAGAAATGTGGATGGTTCTCCTGTTCCATTCGGATGGGATAGCATGCATGCCGACATCAACATACTTGTTTCTCAATGGGTTATGACTGATCCAGCCAATTCCAATCCGTTCATTCCCAAAGCTGACCAGCTAATATGCTCCATTCTGCCTGAATCACCAGCTCCCAAATCAGTCACGTATTCACCAGGTGGACTCTTGTTCAAACCTGGATCTTGTAACCTGCAACACATGACATCCTTATCATTTCTTCTTATAGTTTATGCTCGATACCTGAAAGGTGCCAACAGAACCATAAACTGTGGCAATAATGTTGTTCTTGGTCCAGCTCATTTGATAAATTTCACAAGAAAACAGGTTGATTATATACTCGGAAGCAACCCATTGGGGATGTCTTATATGGTGGGATATGGTACAAAATTTCCTCAAAGAATACATCATCGTGCTTCTGTGGTACCTTCCATGGATAAGCATCCACAGCACATGAGGTGCCATGACGGTGATCAGTATTTCAAAAGCCAAGCTCCGAATCAAAACTTGCTAATAGGAGCAGTTGTCGGGGGACCAGCCGAGAATGATACATTTGAAGATTCTCGATATGATCCTGGACAATCAGAGCCAGTTACATACAACAATGCCCCTTTTGTTGGTGTTTTGGCATTCTTTAAGGCTCTATATAATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

478

Amino Acids

52.93

Weight (kDa)

6.7

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 469 1e-116 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 981
Acc65I GGTACC 1 cut(s) 1204
AccB1I GGYRCC 3 cut(s) 1031, 1204, 1239
AciI CCGC 3 cut(s) 63, 66, 485
AclWI GGATC 7 cut(s) 219, 331, 386, 613, 836, 970, 1346
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 3 cut(s) 151, 1090, 1169
AcuI CTGAAG 1 cut(s) 438
AdeI CACNNNGTG 1 cut(s) 941
AfaI GTAC 3 cut(s) 7, 1165, 1206
AfiI CCNNNNNNNGG 1 cut(s) 1397
AflII CTTAAG 1 cut(s) 560
AgsI TTSAA 7 cut(s) 125, 256, 335, 584, 955, 1264, 1337
AhlI ACTAGT 1 cut(s) 617
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 5 cut(s) 394, 466, 937, 958, 1354
AleI CACNNNNGTG 1 cut(s) 446
AluBI AGCT 8 cut(s) 350, 416, 509, 875, 883, 914, 1079, 1274
AluI AGCT 8 cut(s) 350, 416, 509, 875, 883, 914, 1079, 1274
Alw26I GTCTC 1 cut(s) 621
AlwI GGATC 7 cut(s) 219, 331, 386, 613, 836, 970, 1346
AoxI GGCC 2 cut(s) 127, 267
ApeKI GCWGC 4 cut(s) 38, 197, 494, 506
ApoI RAATTY 3 cut(s) 151, 1090, 1169
Asp718I GGTACC 1 cut(s) 1204
AspS9I GGNCC 2 cut(s) 1073, 1313
AsuHPI GGTGA 4 cut(s) 251, 900, 927, 1262
AvaII GGWCC 2 cut(s) 1073, 1313
BanI GGYRCC 3 cut(s) 1031, 1204, 1239
BbsI GAAGAC 1 cut(s) 426
BbvI GCAGC 4 cut(s) 50, 209, 506, 518
BccI CCATC 6 cut(s) 187, 313, 351, 578, 758, 780
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 5 cut(s) 396, 468, 939, 960, 1356
BciVI GTATCC 1 cut(s) 421
BclI TGATCA 1 cut(s) 1252
BcoDI GTCTC 1 cut(s) 621
BcuI ACTAGT 1 cut(s) 617
BfaI CTAG 2 cut(s) 302, 618
BfmI CTRYAG 1 cut(s) 516
BfrI CTTAAG 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 981
BfuI GTATCC 1 cut(s) 421
BglII AGATCT 1 cut(s) 134
BisI GCNGC 5 cut(s) 39, 66, 198, 495, 507
BlsI GCNGC 5 cut(s) 40, 67, 199, 496, 508
Bme1390I CCNGG 5 cut(s) 396, 468, 939, 960, 1356
Bme18I GGWCC 2 cut(s) 1073, 1313
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 2 cut(s) 1073, 1313
BmiI GGNNCC 6 cut(s) 634, 690, 1033, 1206, 1241, 1314
BmrFI CCNGG 5 cut(s) 396, 468, 939, 960, 1356
BmsI GCATC 3 cut(s) 85, 223, 1231
BpiI GAAGAC 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 752
BsaAI YACGTR 1 cut(s) 930
BsaBI GATNNNNATC 2 cut(s) 597, 1221
BsaJI CCNNGG 2 cut(s) 264, 1212
BsaXI ACNNNNNCTCC 2 cut(s) 1290, 1320
Bsc4I CCNNNNNNNGG 1 cut(s) 1397
Bse1I ACTGG 1 cut(s) 1370
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 2 cut(s) 597, 1221
BseBI CCWGG 5 cut(s) 396, 468, 939, 960, 1356
BseDI CCNNGG 2 cut(s) 264, 1212
BseGI GGATG 6 cut(s) 570, 769, 791, 986, 1146, 1222
BseJI GATNNNNATC 2 cut(s) 597, 1221
BseLI CCNNNNNNNGG 1 cut(s) 1397
BseMI GCAATG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 1370
BseXI GCAGC 4 cut(s) 50, 209, 506, 518
BshFI GGCC 2 cut(s) 129, 269
BshNI GGYRCC 3 cut(s) 1031, 1204, 1239
BslFI GGGAC 1 cut(s) 1326
BslI CCNNNNNNNGG 1 cut(s) 1397
BsmAI GTCTC 1 cut(s) 621
BsmBI CGTCTC 1 cut(s) 621
BsmFI GGGAC 1 cut(s) 1326
BsmI GAATGC 1 cut(s) 1409
BsnI GGCC 2 cut(s) 129, 269
Bsp19I CCATGG 2 cut(s) 264, 1212
BspACI CCGC 3 cut(s) 63, 66, 485
BspANI GGCC 2 cut(s) 129, 269
BspLI GGNNCC 6 cut(s) 634, 690, 1033, 1206, 1241, 1314
BspMI ACCTGC 1 cut(s) 981
BspPI GGATC 7 cut(s) 219, 331, 386, 613, 836, 970, 1346
BspT107I GGYRCC 3 cut(s) 1031, 1204, 1239
BspTI CTTAAG 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 1370
BssECI CCNNGG 2 cut(s) 264, 1212
BssT1I CCWWGG 2 cut(s) 264, 1212
Bst2UI CCWGG 5 cut(s) 396, 468, 939, 960, 1356
Bst4CI ACNGT 2 cut(s) 1052, 1250
BstAFI CTTAAG 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 930
BstC8I GCNNGC 5 cut(s) 504, 567, 637, 797, 801
BstDSI CCRYGG 2 cut(s) 264, 1212
BstEII GGTNACC 1 cut(s) 224
BstF5I GGATG 6 cut(s) 570, 769, 791, 986, 1146, 1222
BstMAI GTCTC 1 cut(s) 621
BstMWI GCNNNNNNNGC 6 cut(s) 65, 347, 491, 503, 562, 1228
BstNI CCWGG 5 cut(s) 396, 468, 939, 960, 1356
BstNSI RCATGY 4 cut(s) 341, 670, 799, 803
BstPI GGTNACC 1 cut(s) 224
BstSCI CCNGG 5 cut(s) 394, 466, 937, 958, 1354
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 4 cut(s) 50, 209, 506, 518
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 5 cut(s) 134, 211, 323, 605, 962
BstXI CCANNNNNNTGG 5 cut(s) 137, 236, 448, 699, 786
BstYI RGATCY 5 cut(s) 134, 211, 323, 605, 962
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 2 cut(s) 129, 269
BtgI CCRYGG 2 cut(s) 264, 1212
BtgZI GCGATG 1 cut(s) 215
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 6 cut(s) 570, 769, 791, 986, 1146, 1222
BveI ACCTGC 1 cut(s) 981
Cac8I GCNNGC 5 cut(s) 504, 567, 637, 797, 801
Cfr13I GGNCC 2 cut(s) 1073, 1313
CsiI ACCWGGT 2 cut(s) 466, 937
Csp6I GTAC 3 cut(s) 6, 1164, 1205
CviQI GTAC 3 cut(s) 6, 1164, 1205
DraIII CACNNNGTG 1 cut(s) 941
EaeI YGGCCR 1 cut(s) 267
Eco130I CCWWGG 2 cut(s) 264, 1212
Eco47I GGWCC 2 cut(s) 1073, 1313
Eco57I CTGAAG 1 cut(s) 438
Eco91I GGTNACC 1 cut(s) 224
EcoO65I GGTNACC 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 5 cut(s) 394, 466, 937, 958, 1354
EcoT14I CCWWGG 2 cut(s) 264, 1212
EcoT22I ATGCAT 1 cut(s) 801
ErhI CCWWGG 2 cut(s) 264, 1212
Esp3I CGTCTC 1 cut(s) 621
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 1 cut(s) 1326
FauNDI CATATG 1 cut(s) 445
FbaI TGATCA 1 cut(s) 1252
Fnu4HI GCNGC 5 cut(s) 39, 66, 198, 495, 507
FokI GGATG 6 cut(s) 557, 776, 798, 973, 1153, 1209
Fsp4HI GCNGC 5 cut(s) 39, 66, 198, 495, 507
FspBI CTAG 2 cut(s) 302, 618
GluI GCNGC 5 cut(s) 39, 66, 198, 495, 507
GsuI CTGGAG 1 cut(s) 752
HaeIII GGCC 2 cut(s) 129, 269
HinfI GANTC 7 cut(s) 76, 551, 734, 905, 945, 1279, 1340
HphI GGTGA 4 cut(s) 251, 900, 927, 1262
Hpy166II GTNNAC 3 cut(s) 6, 111, 944
Hpy188I TCNGA 7 cut(s) 328, 475, 550, 785, 1124, 1278, 1366
Hpy188III TCNNGA 3 cut(s) 302, 731, 1344
Hpy8I GTNNAC 3 cut(s) 6, 111, 944
HpyAV CCTTC 3 cut(s) 26, 119, 1218
HpyCH4III ACNGT 2 cut(s) 1052, 1250
HpyCH4IV ACGT 2 cut(s) 423, 929
HpyCH4V TGCA 5 cut(s) 494, 506, 590, 799, 976
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 347, 491, 503, 562, 1228
HpySE526I ACGT 2 cut(s) 423, 929
KpnI GGTACC 1 cut(s) 1208
Ksp22I TGATCA 1 cut(s) 1252
LmnI GCTCC 6 cut(s) 294, 632, 896, 919, 1279, 1298
Lsp1109I GCAGC 4 cut(s) 50, 209, 506, 518
LweI GCATC 3 cut(s) 85, 223, 1231
MabI ACCWGGT 2 cut(s) 466, 937
MaeI CTAG 2 cut(s) 302, 618
MaeII ACGT 2 cut(s) 423, 929
MaeIII GTNAC 9 cut(s) 73, 144, 224, 239, 271, 451, 925, 968, 1372
MboII GAAGA 3 cut(s) 431, 992, 1349
MflI RGATCY 5 cut(s) 134, 211, 323, 605, 962
MluCI AATT 9 cut(s) 10, 151, 166, 584, 694, 850, 1090, 1169, 1429
MlyI GAGTC 4 cut(s) 70, 545, 743, 939
MnlI CCTC 4 cut(s) 165, 523, 1185, 1230
Mph1103I ATGCAT 1 cut(s) 801
MseI TTAA 5 cut(s) 23, 378, 525, 561, 1416
MslI CAYNNNNRTG 6 cut(s) 446, 575, 749, 784, 1191, 1248
MspCI CTTAAG 1 cut(s) 560
MspR9I CCNGG 5 cut(s) 396, 468, 939, 960, 1356
Mva1269I GAATGC 1 cut(s) 1409
MvaI CCWGG 5 cut(s) 396, 468, 939, 960, 1356
MwoI GCNNNNNNNGC 6 cut(s) 65, 347, 491, 503, 562, 1228
NcoI CCATGG 2 cut(s) 264, 1212
NdeI CATATG 1 cut(s) 445
NlaIV GGNNCC 6 cut(s) 634, 690, 1033, 1206, 1241, 1314
NmeAIII GCCGAG 1 cut(s) 1345
NmuCI GTSAC 4 cut(s) 73, 239, 271, 925
NsiI ATGCAT 1 cut(s) 801
NspI RCATGY 4 cut(s) 341, 670, 799, 803
OliI CACNNNNGTG 1 cut(s) 446
PaeI GCATGC 2 cut(s) 799, 803
PctI GAATGC 1 cut(s) 1409
PfeI GAWTC 3 cut(s) 905, 1279, 1340
PfoI TCCNGGA 1 cut(s) 1354
PkrI GCNGC 5 cut(s) 40, 67, 199, 496, 508
PleI GAGTC 4 cut(s) 70, 545, 742, 939
PpsI GAGTC 4 cut(s) 70, 545, 742, 939
Ppu21I YACGTR 1 cut(s) 930
Psp6I CCWGG 5 cut(s) 394, 466, 937, 958, 1354
PspEI GGTNACC 1 cut(s) 224
PspGI CCWGG 5 cut(s) 394, 466, 937, 958, 1354
PspN4I GGNNCC 6 cut(s) 634, 690, 1033, 1206, 1241, 1314
PspPI GGNCC 2 cut(s) 1073, 1313
PsuI RGATCY 5 cut(s) 134, 211, 323, 605, 962
RsaI GTAC 3 cut(s) 7, 1165, 1206
RsaNI GTAC 3 cut(s) 6, 1164, 1205
RseI CAYNNNNRTG 6 cut(s) 446, 575, 749, 784, 1191, 1248
SaqAI TTAA 5 cut(s) 23, 378, 525, 561, 1416
SatI GCNGC 5 cut(s) 39, 66, 198, 495, 507
Sau96I GGNCC 2 cut(s) 1073, 1313
SchI GAGTC 4 cut(s) 70, 545, 743, 939
ScrFI CCNGG 5 cut(s) 396, 468, 939, 960, 1356
SexAI ACCWGGT 2 cut(s) 466, 937
SfaNI GCATC 3 cut(s) 85, 223, 1231
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 2 cut(s) 1073, 1313
SmiMI CAYNNNNRTG 6 cut(s) 446, 575, 749, 784, 1191, 1248
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
SpeI ACTAGT 1 cut(s) 617
SphI GCATGC 2 cut(s) 799, 803
Sse9I AATT 9 cut(s) 10, 151, 166, 584, 694, 850, 1090, 1169, 1429
SsiI CCGC 3 cut(s) 63, 66, 485
SspMI CTAG 2 cut(s) 302, 618
StyD4I CCNGG 5 cut(s) 394, 466, 937, 958, 1354
StyI CCWWGG 2 cut(s) 264, 1212
TaaI ACNGT 2 cut(s) 1052, 1250
TaiI ACGT 2 cut(s) 426, 932
TaqI TCGA 3 cut(s) 104, 1018, 1345
TasI AATT 9 cut(s) 10, 151, 166, 584, 694, 850, 1090, 1169, 1429
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 905, 1279, 1340
Tru1I TTAA 5 cut(s) 23, 378, 525, 561, 1416
Tru9I TTAA 5 cut(s) 23, 378, 525, 561, 1416
TseFI GTSAC 4 cut(s) 73, 239, 271, 925
TseI GCWGC 4 cut(s) 38, 197, 494, 506
Tsp45I GTSAC 4 cut(s) 73, 239, 271, 925
TspDTI ATGAA 3 cut(s) 179, 196, 853
TspGWI ACGGA 2 cut(s) 645, 849
Vha464I CTTAAG 1 cut(s) 560
VpaK11BI GGWCC 2 cut(s) 1073, 1313
XapI RAATTY 3 cut(s) 151, 1090, 1169
XbaI TCTAGA 1 cut(s) 301
XceI RCATGY 4 cut(s) 341, 670, 799, 803
XspI CTAG 2 cut(s) 302, 618
Zsp2I ATGCAT 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.