Rw1G035040

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000663
Physical Location & Seq
Forward (+)
62898853 .. 62901239
2387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035040.1

Sequence Viewer

Length: 1419 bp
ATGTGCAGGAATACCCTTCCAAGGGTGTTAATAATATTAGCAGGGATGCTCACAGCAGCAAGTTTGGCTTCGTCACACAACTACACTGATGCTTTGTCGAAGTGCATATTGTTTTTCGAAGGGCAGAGATCTGGAAACTTACCGCCTTCGCAAAGAATGACTTGGAGGAAAGATTCGGCCTTGAAGGATGGCTCTGATGTTGGTATGGATCTTGTAGGGGGTTACTATGATGCAGGAGACAATGTGAAGTTCAACTTTCCCATGGCCTTCACAACAACAATGTTGTCGTGGAGCGTGCTAGAGTTCGGAAATCTAATGGGATCGGACCTTCAACATGCTATGGAAGCTATAAGATGGGGGACTGATTATTTTCTGAAAGCCACAAACGTTCCTGATTCGGTTGTGGGTGTAGTTGGCGATCCCATTGGTGACCACAGTTGTTCTCAAAGGCCTGAGGACATGGACACCCCAAGAACCTCTTATCTAGTCACCAAAGAAAAGCCAGGTTCAGAAGTCTCAGCCGAAATTGCAGCTGCATTTGCAGCTTCTTCCTTGGTGTTCAAAGATTCAGACAATAACTACTCTACTTTGCTTCTTAACCGATCAATGCAGGTCTTTGAATTTGCAGATAAGTATAGGGGGTCTTACAATGACAGTATTGGGGAAGGAGCATGCCCCTTTTACTGTGATTTTAGTGGCTACATGGATGAATTGCTCTGGGGAGCTTCTTGGCTGTACAAGGCAACCGGAGCAGCTGATTATTGGAATTATAAATTTCAATGGGGATCCTGTTTCATACATAAATGGTGCTTCACTGAATTTGGATGGGATTCAAAGCATTCCGGGATTAACGTACTTGTTTCTCAGTGGGTAATGACTGATCCGAACAATTCTAGTCCTTTTATTCTCAATGCGGATAAGTTTGTATGCTCGATTTTGCCAGAATCACCTACTCAAAAATCGGTCACATATTCACCAGGAGGGCTGTTGTTCAAACCAGGAGGAAGTAACCTGCAACATTCAACCTCAATATCATATCTTCTTATGGTTTATGCTGGGTACATGAAAGCTACGAACAAAGTCGTAATTGTTGATTATATACTAGGAAGCAACCCATTAGGCATGTCATACATGGTCGGATTCGGGCCACAGTTTCCTCAGAAAATACATCACCGCGGTTCTGTTCTACCATCCCTTGATCAACACCCACAACACATCGATTGCCGCGGAGGAGACGTGTATTGGCAAAGCCAAGGTCCTAATCTGAATTTGCTAACAGGTGCAATTGTGGGAGGTCCTGCTGAAAATGATACGTTCCAAGATTCTCGATATAATGTTCCACAATCAGAGCCAACTACATACATCAACGCTCCTTTTGTGGGAGTTTTGGCTTACTTTAAATCACTATTTCCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

472

Amino Acids

52.17

Weight (kDa)

5.36

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 463 1.8e-140 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 771
Acc36I ACCTGC 2 cut(s) 601, 1020
AccII CGCG 2 cut(s) 1176, 1227
AciI CCGC 6 cut(s) 143, 914, 1174, 1176, 1225, 1227
AclI AACGTT 1 cut(s) 387
AclWI GGATC 6 cut(s) 216, 328, 413, 780, 793, 875
AcsI RAATTY 4 cut(s) 620, 773, 818, 1267
AfaI GTAC 3 cut(s) 737, 855, 1061
AfiI CCNNNNNNNGG 3 cut(s) 21, 22, 1379
AflIII ACRYGT 1 cut(s) 1236
AgsI TTSAA 9 cut(s) 184, 253, 332, 562, 620, 779, 834, 994, 1023
AjiI CACGTC 1 cut(s) 1237
AjnI CCWGG 3 cut(s) 502, 976, 997
AluBI AGCT 6 cut(s) 347, 533, 545, 725, 755, 1070
AluI AGCT 6 cut(s) 347, 533, 545, 725, 755, 1070
Alw26I GTCTC 3 cut(s) 231, 520, 1227
AlwI GGATC 6 cut(s) 216, 328, 413, 780, 793, 875
AoxI GGCC 4 cut(s) 177, 264, 449, 1145
ApeKI GCWGC 5 cut(s) 56, 530, 533, 542, 752
ApoI RAATTY 4 cut(s) 620, 773, 818, 1267
ArsI GACNNNNNNTTYG 2 cut(s) 1240, 1272
AspS9I GGNCC 4 cut(s) 325, 1145, 1256, 1295
AsuC2I CCSGG 1 cut(s) 844
AsuHPI GGTGA 5 cut(s) 440, 481, 939, 966, 1163
AsuII TTCGAA 1 cut(s) 117
AvaII GGWCC 3 cut(s) 325, 1256, 1295
AxyI CCTNAGG 1 cut(s) 453
BamHI GGATCC 1 cut(s) 785
BbvI GCAGC 5 cut(s) 68, 520, 542, 554, 764
BccI CCATC 4 cut(s) 182, 348, 819, 1198
BciT130I CCWGG 3 cut(s) 504, 978, 999
BclI TGATCA 1 cut(s) 1198
BcnI CCSGG 1 cut(s) 844
BcoDI GTCTC 3 cut(s) 231, 520, 1227
BfaI CTAG 4 cut(s) 299, 485, 894, 1103
BfuAI ACCTGC 2 cut(s) 601, 1020
BglII AGATCT 1 cut(s) 128
BisI GCNGC 6 cut(s) 57, 531, 534, 543, 753, 1225
BlsI GCNGC 6 cut(s) 58, 532, 535, 544, 754, 1226
Bme1390I CCNGG 4 cut(s) 504, 844, 978, 999
Bme18I GGWCC 3 cut(s) 325, 1256, 1295
BmgBI CACGTC 1 cut(s) 1237
BmgT120I GGNCC 4 cut(s) 325, 1145, 1256, 1295
BmiI GGNNCC 1 cut(s) 787
BmrFI CCNGG 4 cut(s) 504, 844, 978, 999
BmsI GCATC 3 cut(s) 36, 79, 220
Bpu14I TTCGAA 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 844
Bsa29I ATCGAT 1 cut(s) 1218
BsaJI CCNNGG 6 cut(s) 20, 261, 552, 1174, 1225, 1252
BsaWI WCCGGW 1 cut(s) 746
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 1221, 1251
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 22, 1379
Bse21I CCTNAGG 1 cut(s) 453
BseBI CCWGG 3 cut(s) 504, 978, 999
BseCI ATCGAT 1 cut(s) 1218
BseDI CCNNGG 6 cut(s) 20, 261, 552, 1174, 1225, 1252
BseGI GGATG 5 cut(s) 51, 193, 712, 830, 1190
BseLI CCNNNNNNNGG 3 cut(s) 21, 22, 1379
BseMII CTCAG 4 cut(s) 444, 531, 878, 1172
BseRI GAGGAG 1 cut(s) 1245
BseXI GCAGC 5 cut(s) 68, 520, 542, 554, 764
BseYI CCCAGC 1 cut(s) 1055
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 2 cut(s) 1176, 1227
BshFI GGCC 4 cut(s) 179, 266, 451, 1147
BshVI ATCGAT 1 cut(s) 1218
BsiSI CCGG 2 cut(s) 747, 843
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 3 cut(s) 21, 22, 1379
BsmAI GTCTC 3 cut(s) 231, 520, 1227
BsmBI CGTCTC 1 cut(s) 1227
BsmFI GGGAC 1 cut(s) 373
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 4 cut(s) 179, 266, 451, 1147
Bsp119I TTCGAA 1 cut(s) 117
Bsp1407I TGTACA 1 cut(s) 735
Bsp143I GATC 8 cut(s) 128, 208, 320, 418, 602, 785, 880, 1198
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 6 cut(s) 143, 914, 1174, 1176, 1225, 1227
BspANI GGCC 4 cut(s) 179, 266, 451, 1147
BspCNI CTCAG 4 cut(s) 445, 530, 877, 1171
BspDI ATCGAT 1 cut(s) 1218
BspFNI CGCG 2 cut(s) 1176, 1227
BspLI GGNNCC 1 cut(s) 787
BspMI ACCTGC 2 cut(s) 601, 1020
BspPI GGATC 6 cut(s) 216, 328, 413, 780, 793, 875
BspT104I TTCGAA 1 cut(s) 117
BsrGI TGTACA 1 cut(s) 735
BssECI CCNNGG 6 cut(s) 20, 261, 552, 1174, 1225, 1252
BssMI GATC 8 cut(s) 128, 208, 320, 418, 602, 785, 880, 1198
BssT1I CCWWGG 4 cut(s) 20, 261, 552, 1252
Bst2UI CCWGG 3 cut(s) 504, 978, 999
Bst4CI ACNGT 4 cut(s) 437, 656, 686, 1152
BstAUI TGTACA 1 cut(s) 735
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 296, 673
BstDEI CTNAG 4 cut(s) 453, 517, 864, 1158
BstDSI CCRYGG 3 cut(s) 261, 1174, 1225
BstEII GGTNACC 1 cut(s) 428
BstF5I GGATG 5 cut(s) 51, 193, 712, 830, 1190
BstFNI CGCG 2 cut(s) 1176, 1227
BstKTI GATC 8 cut(s) 131, 211, 323, 421, 605, 788, 883, 1201
BstMAI GTCTC 3 cut(s) 231, 520, 1227
BstMBI GATC 8 cut(s) 128, 208, 320, 418, 602, 785, 880, 1198
BstMWI GCNNNNNNNGC 6 cut(s) 65, 344, 527, 539, 542, 749
BstNI CCWGG 3 cut(s) 504, 978, 999
BstNSI RCATGY 3 cut(s) 338, 675, 1126
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 4 cut(s) 502, 842, 976, 997
BstUI CGCG 2 cut(s) 1176, 1227
BstV1I GCAGC 5 cut(s) 68, 520, 542, 554, 764
BstX2I RGATCY 3 cut(s) 128, 208, 785
BstYI RGATCY 3 cut(s) 128, 208, 785
Bsu15I ATCGAT 1 cut(s) 1218
Bsu36I CCTNAGG 1 cut(s) 453
BsuRI GGCC 4 cut(s) 179, 266, 451, 1147
BsuTUI ATCGAT 1 cut(s) 1218
BtgI CCRYGG 3 cut(s) 261, 1174, 1225
BtrI CACGTC 1 cut(s) 1237
BtsCI GGATG 5 cut(s) 51, 193, 712, 830, 1190
BtsIMutI CAGTG 3 cut(s) 84, 813, 872
BveI ACCTGC 2 cut(s) 601, 1020
Cac8I GCNNGC 2 cut(s) 296, 673
Cfr13I GGNCC 4 cut(s) 325, 1145, 1256, 1295
Cfr42I CCGCGG 2 cut(s) 1177, 1228
ClaI ATCGAT 1 cut(s) 1218
Csp6I GTAC 3 cut(s) 736, 854, 1060
CviAII CATG 8 cut(s) 262, 335, 460, 672, 703, 1063, 1123, 1132
CviQI GTAC 3 cut(s) 736, 854, 1060
DdeI CTNAG 4 cut(s) 453, 517, 864, 1158
DpnI GATC 8 cut(s) 130, 210, 322, 420, 604, 787, 882, 1200
DpnII GATC 8 cut(s) 128, 208, 320, 418, 602, 785, 880, 1198
DraI TTTAAA 1 cut(s) 1399
Eco130I CCWWGG 4 cut(s) 20, 261, 552, 1252
Eco147I AGGCCT 1 cut(s) 451
Eco47I GGWCC 3 cut(s) 325, 1256, 1295
Eco81I CCTNAGG 1 cut(s) 453
Eco91I GGTNACC 1 cut(s) 428
EcoO109I RGGNCCY 2 cut(s) 1256, 1295
EcoO65I GGTNACC 1 cut(s) 428
EcoRII CCWGG 3 cut(s) 502, 976, 997
EcoT14I CCWWGG 4 cut(s) 20, 261, 552, 1252
ErhI CCWWGG 4 cut(s) 20, 261, 552, 1252
Esp3I CGTCTC 1 cut(s) 1227
FaeI CATG 8 cut(s) 265, 338, 463, 675, 706, 1066, 1126, 1135
FalI AAGNNNNNCTT 8 cut(s) 52, 84, 145, 177, 239, 271, 463, 495
FaqI GGGAC 1 cut(s) 373
FatI CATG 8 cut(s) 261, 334, 459, 671, 702, 1062, 1122, 1131
FbaI TGATCA 1 cut(s) 1198
Fnu4HI GCNGC 6 cut(s) 57, 531, 534, 543, 753, 1225
FokI GGATG 5 cut(s) 58, 200, 719, 837, 1177
Fsp4HI GCNGC 6 cut(s) 57, 531, 534, 543, 753, 1225
FspBI CTAG 4 cut(s) 299, 485, 894, 1103
GluI GCNGC 6 cut(s) 57, 531, 534, 543, 753, 1225
GsaI CCCAGC 1 cut(s) 1059
HaeIII GGCC 4 cut(s) 179, 266, 451, 1147
HapII CCGG 2 cut(s) 747, 843
Hin1II CATG 8 cut(s) 265, 338, 463, 675, 706, 1066, 1126, 1135
HinfI GANTC 7 cut(s) 173, 395, 566, 830, 944, 1140, 1322
HpaII CCGG 2 cut(s) 747, 843
HphI GGTGA 5 cut(s) 440, 481, 939, 966, 1163
Hpy188III TCNNGA 3 cut(s) 132, 392, 1326
HpyAV CCTTC 7 cut(s) 26, 113, 156, 178, 277, 338, 659
HpyCH4III ACNGT 4 cut(s) 437, 656, 686, 1152
HpyCH4IV ACGT 4 cut(s) 387, 852, 1236, 1313
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 344, 527, 539, 542, 749
HpyF3I CTNAG 4 cut(s) 453, 517, 864, 1158
HpySE526I ACGT 4 cut(s) 387, 852, 1236, 1313
Hsp92II CATG 8 cut(s) 265, 338, 463, 675, 706, 1066, 1126, 1135
Ksp22I TGATCA 1 cut(s) 1198
KspI CCGCGG 2 cut(s) 1177, 1228
Kzo9I GATC 8 cut(s) 128, 208, 320, 418, 602, 785, 880, 1198
LmnI GCTCC 5 cut(s) 291, 668, 722, 749, 1375
Lsp1109I GCAGC 5 cut(s) 68, 520, 542, 554, 764
LweI GCATC 3 cut(s) 36, 79, 220
MaeI CTAG 4 cut(s) 299, 485, 894, 1103
MaeII ACGT 4 cut(s) 387, 852, 1236, 1313
MaeIII GTNAC 6 cut(s) 72, 221, 428, 487, 964, 1007
MalI GATC 8 cut(s) 130, 210, 322, 420, 604, 787, 882, 1200
MboI GATC 8 cut(s) 128, 208, 320, 418, 602, 785, 880, 1198
MboII GAAGA 2 cut(s) 540, 1031
MfeI CAATTG 1 cut(s) 1284
MflI RGATCY 3 cut(s) 128, 208, 785
MmeI TCCRAC 1 cut(s) 1117
MnlI CCTC 9 cut(s) 159, 448, 487, 974, 995, 1036, 1167, 1223, 1286
MseI TTAA 4 cut(s) 29, 597, 849, 1398
MspA1I CMGCKG 4 cut(s) 533, 755, 1176, 1227
MspI CCGG 2 cut(s) 747, 843
MspR9I CCNGG 4 cut(s) 504, 844, 978, 999
MunI CAATTG 1 cut(s) 1284
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 3 cut(s) 504, 978, 999
MvnI CGCG 2 cut(s) 1176, 1227
MwoI GCNNNNNNNGC 6 cut(s) 65, 344, 527, 539, 542, 749
NciI CCSGG 1 cut(s) 844
NcoI CCATGG 1 cut(s) 261
NdeII GATC 8 cut(s) 128, 208, 320, 418, 602, 785, 880, 1198
NlaIII CATG 8 cut(s) 265, 338, 463, 675, 706, 1066, 1126, 1135
NlaIV GGNNCC 1 cut(s) 787
NmuCI GTSAC 4 cut(s) 72, 428, 487, 964
NspI RCATGY 3 cut(s) 338, 675, 1126
NspV TTCGAA 1 cut(s) 117
PaeI GCATGC 1 cut(s) 675
PceI AGGCCT 1 cut(s) 451
PctI GAATGC 1 cut(s) 838
PfeI GAWTC 7 cut(s) 173, 395, 566, 830, 944, 1140, 1322
PfoI TCCNGGA 1 cut(s) 842
PkrI GCNGC 6 cut(s) 58, 532, 535, 544, 754, 1226
PpuMI RGGWCCY 2 cut(s) 1256, 1295
PsiI TTATAA 1 cut(s) 771
Psp1406I AACGTT 1 cut(s) 387
Psp5II RGGWCCY 2 cut(s) 1256, 1295
Psp6I CCWGG 3 cut(s) 502, 976, 997
PspEI GGTNACC 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 3 cut(s) 502, 976, 997
PspN4I GGNNCC 1 cut(s) 787
PspPI GGNCC 4 cut(s) 325, 1145, 1256, 1295
PspPPI RGGWCCY 2 cut(s) 1256, 1295
PsuI RGATCY 3 cut(s) 128, 208, 785
PvuII CAGCTG 2 cut(s) 533, 755
RsaI GTAC 3 cut(s) 737, 855, 1061
RsaNI GTAC 3 cut(s) 736, 854, 1060
SacII CCGCGG 2 cut(s) 1177, 1228
SaqAI TTAA 4 cut(s) 29, 597, 849, 1398
SatI GCNGC 6 cut(s) 57, 531, 534, 543, 753, 1225
Sau3AI GATC 8 cut(s) 128, 208, 320, 418, 602, 785, 880, 1198
Sau96I GGNCC 4 cut(s) 325, 1145, 1256, 1295
ScrFI CCNGG 4 cut(s) 504, 844, 978, 999
SfaNI GCATC 3 cut(s) 36, 79, 220
Sfr303I CCGCGG 2 cut(s) 1177, 1228
SfuI TTCGAA 1 cut(s) 117
SgrBI CCGCGG 2 cut(s) 1177, 1228
SinI GGWCC 3 cut(s) 325, 1256, 1295
SphI GCATGC 1 cut(s) 675
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 6 cut(s) 143, 914, 1174, 1176, 1225, 1227
SspI AATATT 1 cut(s) 36
SspMI CTAG 4 cut(s) 299, 485, 894, 1103
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 4 cut(s) 502, 842, 976, 997
StyI CCWWGG 4 cut(s) 20, 261, 552, 1252
TaaI ACNGT 4 cut(s) 437, 656, 686, 1152
TaiI ACGT 4 cut(s) 390, 855, 1239, 1316
TaqI TCGA 5 cut(s) 98, 117, 932, 1218, 1327
TaqII GACCGA 1 cut(s) 952
TatI WGTACW 1 cut(s) 735
TauI GCSGC 1 cut(s) 1227
TfiI GAWTC 7 cut(s) 173, 395, 566, 830, 944, 1140, 1322
Tru1I TTAA 4 cut(s) 29, 597, 849, 1398
Tru9I TTAA 4 cut(s) 29, 597, 849, 1398
TscAI CASTG 3 cut(s) 91, 820, 872
TseFI GTSAC 4 cut(s) 72, 428, 487, 964
TseI GCWGC 5 cut(s) 56, 530, 533, 542, 752
Tsp45I GTSAC 4 cut(s) 72, 428, 487, 964
TspDTI ATGAA 3 cut(s) 723, 784, 1079
TspRI CASTG 3 cut(s) 91, 820, 872
VpaK11BI GGWCC 3 cut(s) 325, 1256, 1295
XapI RAATTY 4 cut(s) 620, 773, 818, 1267
XceI RCATGY 3 cut(s) 338, 675, 1126
XspI CTAG 4 cut(s) 299, 485, 894, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.