Rroxscaffold_4G00283370

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000663
Physical Location & Seq
Reverse (-)
5290343 .. 5292083
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283370.1

Sequence Viewer

Length: 1236 bp
ATGTTGTCGTGGAGCGTGCTAGAGTTCGGAAATCTAATGGGATCGGACCTTCAACATGCTATGGAAGCTATAAGATGGGGGACTGATTATTTTCTGAAAGCCACAAACGTTCCTGATTCGGTTGTGGGTGTAGTTGGCGATCCCATTGGTGACCACAGTTGTTCTCAAAGGCCTGAGGACATGGACACCCCAAGAACCTCTTATGTAGTCACCAAAGAAAAGCCAGGTTCAGAAGTCTCAGCCGAAATTGCAGCTGCATTTGCAGCTTCTTCCTTGGTGTTCAAAGATTTAGACAATAACTACTCTACTTTGCTTCTTAACCGATCAATGCAGGTCTTTGAATTTGCAGATAAGTATAGGGGGTCTTACAATGACAGTATTGGGGAAGGAGCATGCCCCTTTTACTGTGATTTTAGTGGCTACATGGATGAATTGCTCTGGGGAGCTTCTTGGCTATACAAGGCAACCGGAGCAGCTGATTATTGGAATTATGTGAGAGAGAACATAGACAAAATGGAGTCCACTGTAATAAGAAATTTCAATGGGGATCCTGTTTCGTACATAAATGGTGCTGTCACTGAATTTGGATGGGATTCAAAGCATTCCGGGATTAACGTACTTGTTTCTCAGTGGGTAATGACTGATCCGAACAATTCTAGTCCTTTTATTCTCAATGCGGATAAGTTTGTATGCTCGATTTTGCCAGAATCACCTACTCAAAAATCGGTCACATATTCACCAGGAGGGCTGTTGTTCAAACCAGGAGGAAGTAACCTGCAACATTCAACCTCAATATCATATCTTCTTATGGTTTATGCTGGATACATGAAAGCTACGAACAAAGTGGTGAATTGTGGTAATAATGTGGTCATCACTCCAGATCGGCTAGTAAATTTCACCAAAGGACAGGTTGATTATATACTAGGAAGCAACCCATTAGGCATGTCATACATGGTCGGATTCGGGCCACAGTTTCCTCAGAAAATACATCACCGCGGTTCTGTTCTACCATGCCTTGATCAACACCCACAACACATCGATTGCCGCGGAGGAGACGTGTATTGGCAAAGCCAAGGTCCTAATCTGAATTTGCTAACAGGTGCAATTGTGGGAGGTCCTGCTGAAAATGATACGTTCCAAGATTCTCGATATAATGTTCCACAATCAGAGCCAACTACATACATCAACGCTCCTTTTGTGGGAGTTTTGGCTTACTTTAAATCACTATTTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

411

Amino Acids

45.31

Weight (kDa)

4.94

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 403 5e-109 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 322, 783
AccII CGCG 2 cut(s) 996, 1047
AciI CCGC 5 cut(s) 677, 994, 996, 1045, 1047
AclI AACGTT 1 cut(s) 108
AclWI GGATC 5 cut(s) 49, 134, 542, 555, 638
AcsI RAATTY 5 cut(s) 341, 535, 581, 892, 1087
AfaI GTAC 2 cut(s) 560, 618
AfiI CCNNNNNNNGG 1 cut(s) 1199
AflIII ACRYGT 1 cut(s) 1056
AgsI TTSAA 7 cut(s) 53, 283, 341, 541, 597, 757, 786
AjiI CACGTC 1 cut(s) 1057
AjnI CCWGG 3 cut(s) 223, 739, 760
AluBI AGCT 6 cut(s) 68, 254, 266, 446, 476, 833
AluI AGCT 6 cut(s) 68, 254, 266, 446, 476, 833
Alw26I GTCTC 2 cut(s) 241, 1047
AlwI GGATC 5 cut(s) 49, 134, 542, 555, 638
AoxI GGCC 2 cut(s) 170, 965
ApeKI GCWGC 4 cut(s) 251, 254, 263, 473
ApoI RAATTY 5 cut(s) 341, 535, 581, 892, 1087
ArsI GACNNNNNNTTYG 2 cut(s) 1060, 1092
AspS9I GGNCC 4 cut(s) 46, 965, 1076, 1115
AsuC2I CCSGG 1 cut(s) 607
AsuHPI GGTGA 7 cut(s) 161, 202, 702, 729, 859, 889, 983
AvaII GGWCC 3 cut(s) 46, 1076, 1115
AxyI CCTNAGG 1 cut(s) 174
BamHI GGATCC 1 cut(s) 547
BbvI GCAGC 4 cut(s) 241, 263, 275, 485
BccI CCATC 2 cut(s) 69, 582
BciT130I CCWGG 3 cut(s) 225, 741, 762
BciVI GTATCC 1 cut(s) 815
BclI TGATCA 1 cut(s) 1018
BcnI CCSGG 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 241, 1047
BfaI CTAG 4 cut(s) 20, 657, 887, 923
BfuAI ACCTGC 2 cut(s) 322, 783
BfuI GTATCC 1 cut(s) 815
BisI GCNGC 5 cut(s) 252, 255, 264, 474, 1045
BlsI GCNGC 5 cut(s) 253, 256, 265, 475, 1046
Bme1390I CCNGG 4 cut(s) 225, 607, 741, 762
Bme18I GGWCC 3 cut(s) 46, 1076, 1115
BmgBI CACGTC 1 cut(s) 1057
BmgT120I GGNCC 4 cut(s) 46, 965, 1076, 1115
BmiI GGNNCC 1 cut(s) 549
BmrFI CCNGG 4 cut(s) 225, 607, 741, 762
BpmI CTGGAG 1 cut(s) 861
BpuMI CCSGG 1 cut(s) 607
Bsa29I ATCGAT 1 cut(s) 1038
BsaJI CCNNGG 4 cut(s) 273, 994, 1045, 1072
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 1 cut(s) 1199
Bse21I CCTNAGG 1 cut(s) 174
BseBI CCWGG 3 cut(s) 225, 741, 762
BseCI ATCGAT 1 cut(s) 1038
BseDI CCNNGG 4 cut(s) 273, 994, 1045, 1072
BseGI GGATG 2 cut(s) 433, 593
BseLI CCNNNNNNNGG 1 cut(s) 1199
BseMII CTCAG 4 cut(s) 165, 252, 641, 992
BseRI GAGGAG 1 cut(s) 1065
BseXI GCAGC 4 cut(s) 241, 263, 275, 485
Bsh1236I CGCG 2 cut(s) 996, 1047
BshFI GGCC 2 cut(s) 172, 967
BshVI ATCGAT 1 cut(s) 1038
BsiSI CCGG 2 cut(s) 468, 606
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 1 cut(s) 1199
BsmAI GTCTC 2 cut(s) 241, 1047
BsmBI CGTCTC 1 cut(s) 1047
BsmFI GGGAC 1 cut(s) 94
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 2 cut(s) 172, 967
Bsp143I GATC 7 cut(s) 41, 139, 323, 547, 643, 880, 1018
BspACI CCGC 5 cut(s) 677, 994, 996, 1045, 1047
BspANI GGCC 2 cut(s) 172, 967
BspCNI CTCAG 4 cut(s) 166, 251, 640, 991
BspDI ATCGAT 1 cut(s) 1038
BspFNI CGCG 2 cut(s) 996, 1047
BspLI GGNNCC 1 cut(s) 549
BspMI ACCTGC 2 cut(s) 322, 783
BspPI GGATC 5 cut(s) 49, 134, 542, 555, 638
BssECI CCNNGG 4 cut(s) 273, 994, 1045, 1072
BssMI GATC 7 cut(s) 41, 139, 323, 547, 643, 880, 1018
BssT1I CCWWGG 2 cut(s) 273, 1072
Bst2UI CCWGG 3 cut(s) 225, 741, 762
Bst4CI ACNGT 5 cut(s) 158, 377, 407, 526, 972
BstC8I GCNNGC 2 cut(s) 17, 394
BstDEI CTNAG 4 cut(s) 174, 238, 627, 978
BstDSI CCRYGG 2 cut(s) 994, 1045
BstEII GGTNACC 1 cut(s) 149
BstF5I GGATG 2 cut(s) 433, 593
BstFNI CGCG 2 cut(s) 996, 1047
BstKTI GATC 7 cut(s) 44, 142, 326, 550, 646, 883, 1021
BstMAI GTCTC 2 cut(s) 241, 1047
BstMBI GATC 7 cut(s) 41, 139, 323, 547, 643, 880, 1018
BstMWI GCNNNNNNNGC 5 cut(s) 65, 248, 260, 263, 470
BstNI CCWGG 3 cut(s) 225, 741, 762
BstNSI RCATGY 3 cut(s) 59, 396, 946
BstPI GGTNACC 1 cut(s) 149
BstSCI CCNGG 4 cut(s) 223, 605, 739, 760
BstUI CGCG 2 cut(s) 996, 1047
BstV1I GCAGC 4 cut(s) 241, 263, 275, 485
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 1038
Bsu36I CCTNAGG 1 cut(s) 174
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 2 cut(s) 172, 967
BsuTUI ATCGAT 1 cut(s) 1038
BtgI CCRYGG 2 cut(s) 994, 1045
BtrI CACGTC 1 cut(s) 1057
BtsCI GGATG 2 cut(s) 433, 593
BtsIMutI CAGTG 3 cut(s) 522, 576, 635
BveI ACCTGC 2 cut(s) 322, 783
Cac8I GCNNGC 2 cut(s) 17, 394
Cfr13I GGNCC 4 cut(s) 46, 965, 1076, 1115
Cfr42I CCGCGG 2 cut(s) 997, 1048
ClaI ATCGAT 1 cut(s) 1038
Csp6I GTAC 2 cut(s) 559, 617
CviAII CATG 8 cut(s) 56, 181, 393, 424, 826, 943, 952, 1011
CviQI GTAC 2 cut(s) 559, 617
DdeI CTNAG 4 cut(s) 174, 238, 627, 978
DpnI GATC 7 cut(s) 43, 141, 325, 549, 645, 882, 1020
DpnII GATC 7 cut(s) 41, 139, 323, 547, 643, 880, 1018
DraI TTTAAA 1 cut(s) 1219
Eco130I CCWWGG 2 cut(s) 273, 1072
Eco147I AGGCCT 1 cut(s) 172
Eco47I GGWCC 3 cut(s) 46, 1076, 1115
Eco81I CCTNAGG 1 cut(s) 174
Eco91I GGTNACC 1 cut(s) 149
EcoO109I RGGNCCY 2 cut(s) 1076, 1115
EcoO65I GGTNACC 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 223, 739, 760
EcoT14I CCWWGG 2 cut(s) 273, 1072
ErhI CCWWGG 2 cut(s) 273, 1072
Esp3I CGTCTC 1 cut(s) 1047
FaeI CATG 8 cut(s) 59, 184, 396, 427, 829, 946, 955, 1014
FalI AAGNNNNNCTT 2 cut(s) 184, 216
FaqI GGGAC 1 cut(s) 94
FatI CATG 8 cut(s) 55, 180, 392, 423, 825, 942, 951, 1010
FbaI TGATCA 1 cut(s) 1018
Fnu4HI GCNGC 5 cut(s) 252, 255, 264, 474, 1045
FokI GGATG 2 cut(s) 440, 600
Fsp4HI GCNGC 5 cut(s) 252, 255, 264, 474, 1045
FspBI CTAG 4 cut(s) 20, 657, 887, 923
GluI GCNGC 5 cut(s) 252, 255, 264, 474, 1045
GsuI CTGGAG 1 cut(s) 861
HaeIII GGCC 2 cut(s) 172, 967
HapII CCGG 2 cut(s) 468, 606
Hin1II CATG 8 cut(s) 59, 184, 396, 427, 829, 946, 955, 1014
HinfI GANTC 6 cut(s) 116, 518, 593, 707, 960, 1142
HpaII CCGG 2 cut(s) 468, 606
HphI GGTGA 7 cut(s) 161, 202, 702, 729, 859, 889, 983
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 9 cut(s) 29, 46, 96, 232, 648, 959, 981, 1086, 1168
Hpy188III TCNNGA 3 cut(s) 113, 878, 1146
Hpy8I GTNNAC 1 cut(s) 522
HpyAV CCTTC 2 cut(s) 59, 380
HpyCH4III ACNGT 5 cut(s) 158, 377, 407, 526, 972
HpyCH4IV ACGT 4 cut(s) 108, 615, 1056, 1133
HpyCH4V TGCA 7 cut(s) 251, 257, 263, 331, 347, 778, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 248, 260, 263, 470
HpyF3I CTNAG 4 cut(s) 174, 238, 627, 978
HpySE526I ACGT 4 cut(s) 108, 615, 1056, 1133
Hsp92II CATG 8 cut(s) 59, 184, 396, 427, 829, 946, 955, 1014
Ksp22I TGATCA 1 cut(s) 1018
KspI CCGCGG 2 cut(s) 997, 1048
Kzo9I GATC 7 cut(s) 41, 139, 323, 547, 643, 880, 1018
LmnI GCTCC 5 cut(s) 12, 389, 443, 470, 1195
Lsp1109I GCAGC 4 cut(s) 241, 263, 275, 485
MaeI CTAG 4 cut(s) 20, 657, 887, 923
MaeII ACGT 4 cut(s) 108, 615, 1056, 1133
MaeIII GTNAC 5 cut(s) 149, 208, 574, 727, 770
MalI GATC 7 cut(s) 43, 141, 325, 549, 645, 882, 1020
MboI GATC 7 cut(s) 41, 139, 323, 547, 643, 880, 1018
MboII GAAGA 2 cut(s) 261, 794
MfeI CAATTG 1 cut(s) 1104
MflI RGATCY 1 cut(s) 547
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 937
MnlI CCTC 8 cut(s) 169, 208, 737, 758, 799, 987, 1043, 1106
MseI TTAA 3 cut(s) 318, 612, 1218
MspA1I CMGCKG 4 cut(s) 254, 476, 996, 1047
MspI CCGG 2 cut(s) 468, 606
MspR9I CCNGG 4 cut(s) 225, 607, 741, 762
MunI CAATTG 1 cut(s) 1104
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 3 cut(s) 225, 741, 762
MvnI CGCG 2 cut(s) 996, 1047
MwoI GCNNNNNNNGC 5 cut(s) 65, 248, 260, 263, 470
NciI CCSGG 1 cut(s) 607
NdeII GATC 7 cut(s) 41, 139, 323, 547, 643, 880, 1018
NlaIII CATG 8 cut(s) 59, 184, 396, 427, 829, 946, 955, 1014
NlaIV GGNNCC 1 cut(s) 549
NmuCI GTSAC 4 cut(s) 149, 208, 574, 727
NspI RCATGY 3 cut(s) 59, 396, 946
PaeI GCATGC 1 cut(s) 396
PceI AGGCCT 1 cut(s) 172
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 5 cut(s) 116, 593, 707, 960, 1142
PfoI TCCNGGA 1 cut(s) 605
PkrI GCNGC 5 cut(s) 253, 256, 265, 475, 1046
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PpuMI RGGWCCY 2 cut(s) 1076, 1115
Psp1406I AACGTT 1 cut(s) 108
Psp5II RGGWCCY 2 cut(s) 1076, 1115
Psp6I CCWGG 3 cut(s) 223, 739, 760
PspEI GGTNACC 1 cut(s) 149
PspGI CCWGG 3 cut(s) 223, 739, 760
PspN4I GGNNCC 1 cut(s) 549
PspPI GGNCC 4 cut(s) 46, 965, 1076, 1115
PspPPI RGGWCCY 2 cut(s) 1076, 1115
PsuI RGATCY 1 cut(s) 547
PvuII CAGCTG 2 cut(s) 254, 476
RsaI GTAC 2 cut(s) 560, 618
RsaNI GTAC 2 cut(s) 559, 617
SacII CCGCGG 2 cut(s) 997, 1048
SaqAI TTAA 3 cut(s) 318, 612, 1218
SatI GCNGC 5 cut(s) 252, 255, 264, 474, 1045
Sau3AI GATC 7 cut(s) 41, 139, 323, 547, 643, 880, 1018
Sau96I GGNCC 4 cut(s) 46, 965, 1076, 1115
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 4 cut(s) 225, 607, 741, 762
Sfr303I CCGCGG 2 cut(s) 997, 1048
SgrBI CCGCGG 2 cut(s) 997, 1048
SinI GGWCC 3 cut(s) 46, 1076, 1115
SphI GCATGC 1 cut(s) 396
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 5 cut(s) 677, 994, 996, 1045, 1047
SspMI CTAG 4 cut(s) 20, 657, 887, 923
StuI AGGCCT 1 cut(s) 172
StyD4I CCNGG 4 cut(s) 223, 605, 739, 760
StyI CCWWGG 2 cut(s) 273, 1072
TaaI ACNGT 5 cut(s) 158, 377, 407, 526, 972
TaiI ACGT 4 cut(s) 111, 618, 1059, 1136
TaqI TCGA 3 cut(s) 695, 1038, 1147
TaqII GACCGA 1 cut(s) 715
TauI GCSGC 1 cut(s) 1047
TfiI GAWTC 5 cut(s) 116, 593, 707, 960, 1142
Tru1I TTAA 3 cut(s) 318, 612, 1218
Tru9I TTAA 3 cut(s) 318, 612, 1218
TscAI CASTG 3 cut(s) 529, 583, 635
TseFI GTSAC 4 cut(s) 149, 208, 574, 727
TseI GCWGC 4 cut(s) 251, 254, 263, 473
Tsp45I GTSAC 4 cut(s) 149, 208, 574, 727
TspDTI ATGAA 2 cut(s) 444, 842
TspRI CASTG 3 cut(s) 529, 583, 635
VpaK11BI GGWCC 3 cut(s) 46, 1076, 1115
XapI RAATTY 5 cut(s) 341, 535, 581, 892, 1087
XceI RCATGY 3 cut(s) 59, 396, 946
XspI CTAG 4 cut(s) 20, 657, 887, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.