Rw1G035060

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000663
Physical Location & Seq
Forward (+)
62910732 .. 62919130
8399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035060.1

Sequence Viewer

Length: 1533 bp
ATGAGCAAATTAGTCATGTCTAGTAGTAATATTAAACAACCATTAAGGTTGTTGATGGTAGAAGAAGGGATGTTGGCAATGGCAACACTAGTAAGCTCTGCGGTTCAACATAACTACGCGGATGCTTTGTCAAAAAGTATTTTGTTTTTTGAAGGGCAGAGATCGGGTAGGTTGCCGCATACACAACGAATGATATGGAGGAAAGATTCGGCTCTACGTGATGGCCTAGATGTTAAAAGGGATCTGACCGGGGGTTACTATGATGCGGGTGACAATGTGAAATTTAACTTTCCAATGGCTTTCACCACAACAATGTTGTCATGGAGTGTTATAAATTCGGAAAATCAATGGGCTCAGAACTTCCACATGCCTTGGAACTTGGAAGCCATTAGATGGGCGACTGACTATTTGCTTAAATCCACTAGTGCTAGTGGTTCTGTTGTTGGTGTAGTCGGTGATCCCAATGGTGACCACACTTGTTGGGAAAGGCCTGAAGACATGGACACTCCTCGAACATCTTACATAGTAACCACACAAAAGCCAGGTTCTAAAGTTTCAACAGAGACTGCAGCCGCATTTGCAGCTTCTTCCATAGTGTTTAGTGGTTCTGACAAAGGATATTCTACCTTGCTTCTTACCCGAGCAAGACAGGTGTTTGAATTTGCTGATAAATATAGGCGTTCTTACAATGACAGTATTGGGAGTGGAGCATGCCCATTTTACTGTGACTTTAATGGCTACAAGGATGAATTGATTTGGGGAGCAGCATGGTTATACAAGGCAACAAGGAGTCCTATTTATTGGAACTATGTAAAACAGAACATAGATGCCATGAATTTGGAGTCCAATGTAGTCCTAAGAAATGTGGAAGGCCAAGCAGTTGTATACAATGGCGGAGATGTTACCGAATTTGGATGGGATGCAAAGCCTGCTGGCATTAACATCCTTGTTTCTCAACTAAATACTTTTTTGTTCTTTGTTAATTTGTATTGGGTGATGACTAACCCCGTCGAGGCCAATCCCTTCATTCCCAAAGCGGATGGGCTGGTTTGCTCCATTGTGCCCCCGAATCACCTGCTCCCAAATCAATTATTCCAATCTCTTCCTCTGTTCTTTAGTAACATGCAACATACAACTGCTTTATCTTTTCTTTTAGTAGTTTATGCTGGCTATATGAAAGCTGCAAGAAAATTTGTAAATTGTGGGAACAATGTTGTTGTCACTCCAGCTAGGCTGGTAGAACTCACAAAAGGACAGGTTGATTATGTACTGGGAAGAAATCCTAATAGCATGTCATACATGGTGGGATATGGTACAAGATTTCCCCAGAAGATACCTCACCACGGATCAGTGATACCTTCGATTGATCAACACCCCAAACACATGCAGCTAATAGGTGTAATCGTTGGAGAACTAGCTCTGGATGATACATTCGAAGATTCTCGATATAATGTTCCACAATCGGAGCCAACCACATACATTGATGCACCTTTTTTTGGTGTTTTGGTTTACTTCAAATCACCATCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

510

Amino Acids

57.01

Weight (kDa)

6.56

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 39 - 502 9.5e-126 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AarI CACCTGC 1 cut(s) 1083
Acc36I ACCTGC 1 cut(s) 1083
AccB7I CCANNNNNTGG 1 cut(s) 393
AccI GTMKAC 1 cut(s) 885
AccII CGCG 1 cut(s) 119
AciI CCGC 7 cut(s) 101, 119, 176, 266, 573, 894, 1037
AclWI GGATC 3 cut(s) 249, 452, 1354
AcsI RAATTY 6 cut(s) 281, 334, 659, 835, 908, 1190
AcuI CTGAAG 1 cut(s) 513
AfaI GTAC 2 cut(s) 1269, 1315
AfiI CCNNNNNNNGG 4 cut(s) 393, 1012, 1343, 1496
AgsI TTSAA 5 cut(s) 107, 152, 558, 659, 1516
AhlI ACTAGT 2 cut(s) 88, 422
AjnI CCWGG 1 cut(s) 541
AluBI AGCT 6 cut(s) 96, 584, 1181, 1229, 1390, 1418
AluI AGCT 6 cut(s) 96, 584, 1181, 1229, 1390, 1418
Alw26I GTCTC 1 cut(s) 557
AlwI GGATC 3 cut(s) 249, 452, 1354
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 639
AoxI GGCC 4 cut(s) 223, 488, 871, 1014
ApeKI GCWGC 5 cut(s) 569, 581, 764, 1181, 1387
ApoI RAATTY 6 cut(s) 281, 334, 659, 835, 908, 1190
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 8 cut(s) 281, 295, 467, 479, 1006, 1064, 1331, 1512
AsuII TTCGAA 1 cut(s) 1434
AvaI CYCGRG 1 cut(s) 639
BaeGI GKGCMC 1 cut(s) 1065
BanII GRGCYC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 501
BbvI GCAGC 5 cut(s) 581, 593, 776, 1168, 1399
BccI CCATC 6 cut(s) 49, 215, 387, 909, 1034, 1531
BcgI CGANNNNNNTGC 2 cut(s) 1057, 1091
BciT130I CCWGG 1 cut(s) 543
BclI TGATCA 1 cut(s) 1366
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 557
BcuI ACTAGT 2 cut(s) 88, 422
BfaI CTAG 7 cut(s) 21, 89, 227, 423, 429, 1230, 1415
BfmI CTRYAG 1 cut(s) 567
BfuAI ACCTGC 1 cut(s) 1083
BisI GCNGC 7 cut(s) 176, 570, 573, 582, 765, 1182, 1388
BlsI GCNGC 7 cut(s) 177, 571, 574, 583, 766, 1183, 1389
Bme1390I CCNGG 2 cut(s) 250, 543
BmeT110I CYCGRG 1 cut(s) 639
BmiI GGNNCC 1 cut(s) 1467
BmrFI CCNGG 2 cut(s) 250, 543
BmrI ACTGGG 1 cut(s) 1280
BmsI GCATC 5 cut(s) 112, 253, 817, 910, 1474
BmuI ACTGGG 1 cut(s) 1280
BpiI GAAGAC 1 cut(s) 501
BpmI CTGGAG 1 cut(s) 1209
Bpu14I TTCGAA 1 cut(s) 1434
BpuMI CCSGG 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 218
BsaJI CCNNGG 3 cut(s) 249, 371, 1342
Bsc4I CCNNNNNNNGG 4 cut(s) 393, 1012, 1343, 1496
Bse1I ACTGG 1 cut(s) 1275
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 543
BseDI CCNNGG 3 cut(s) 249, 371, 1342
BseGI GGATG 8 cut(s) 75, 127, 751, 920, 925, 942, 1045, 1429
BseLI CCNNNNNNNGG 4 cut(s) 393, 1012, 1343, 1496
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 1275
BseRI GAGGAG 1 cut(s) 498
BseSI GKGCMC 1 cut(s) 1065
BseXI GCAGC 5 cut(s) 581, 593, 776, 1168, 1399
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 4 cut(s) 225, 490, 873, 1016
BsiHKCI CYCGRG 1 cut(s) 639
BsiSI CCGG 1 cut(s) 249
BslI CCNNNNNNNGG 4 cut(s) 393, 1012, 1343, 1496
BsmAI GTCTC 1 cut(s) 557
BsnI GGCC 4 cut(s) 225, 490, 873, 1016
BsoBI CYCGRG 1 cut(s) 639
Bsp119I TTCGAA 1 cut(s) 1434
Bsp1286I GDGCHC 2 cut(s) 355, 1065
Bsp143I GATC 5 cut(s) 161, 241, 457, 1346, 1366
BspACI CCGC 7 cut(s) 101, 119, 176, 266, 573, 894, 1037
BspANI GGCC 4 cut(s) 225, 490, 873, 1016
BspCNI CTCAG 1 cut(s) 367
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 1467
BspMAI CTGCAG 1 cut(s) 571
BspMI ACCTGC 1 cut(s) 1083
BspPI GGATC 3 cut(s) 249, 452, 1354
BspT104I TTCGAA 1 cut(s) 1434
BsrDI GCAATG 1 cut(s) 84
BsrI ACTGG 1 cut(s) 1275
BssECI CCNNGG 3 cut(s) 249, 371, 1342
BssMI GATC 5 cut(s) 161, 241, 457, 1346, 1366
BssNAI GTATAC 1 cut(s) 886
BssT1I CCWWGG 1 cut(s) 371
Bst1107I GTATAC 1 cut(s) 886
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 2 cut(s) 695, 725
Bst6I CTCTTC 1 cut(s) 1107
BstAPI GCANNNNNTGC 1 cut(s) 929
BstBAI YACGTR 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 1434
BstC8I GCNNGC 4 cut(s) 712, 930, 934, 1168
BstDEI CTNAG 2 cut(s) 354, 857
BstDSI CCRYGG 1 cut(s) 1342
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 8 cut(s) 75, 127, 751, 920, 925, 942, 1045, 1429
BstFNI CGCG 1 cut(s) 119
BstKTI GATC 5 cut(s) 164, 244, 460, 1349, 1369
BstMAI GTCTC 1 cut(s) 557
BstMBI GATC 5 cut(s) 161, 241, 457, 1346, 1366
BstMWI GCNNNNNNNGC 3 cut(s) 578, 581, 929
BstNI CCWGG 1 cut(s) 543
BstNSI RCATGY 5 cut(s) 370, 714, 1126, 1294, 1387
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 2 cut(s) 248, 541
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 1065
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 5 cut(s) 581, 593, 776, 1168, 1399
BstV2I GAAGAC 1 cut(s) 501
BstX2I RGATCY 1 cut(s) 241
BstXI CCANNNNNNTGG 1 cut(s) 838
BstYI RGATCY 1 cut(s) 241
BstZ17I GTATAC 1 cut(s) 886
BsuRI GGCC 4 cut(s) 225, 490, 873, 1016
BtgI CCRYGG 1 cut(s) 1342
BtsCI GGATG 8 cut(s) 75, 127, 751, 920, 925, 942, 1045, 1429
BtsIMutI CAGTG 1 cut(s) 1356
BveI ACCTGC 1 cut(s) 1083
Cac8I GCNNGC 4 cut(s) 712, 930, 934, 1168
CaiI CAGNNNCTG 1 cut(s) 566
Csp6I GTAC 2 cut(s) 1268, 1314
CspCI CAANNNNNGTGG 6 cut(s) 353, 388, 461, 496, 1462, 1497
CviQI GTAC 2 cut(s) 1268, 1314
DdeI CTNAG 2 cut(s) 354, 857
DpnI GATC 5 cut(s) 163, 243, 459, 1348, 1368
DpnII GATC 5 cut(s) 161, 241, 457, 1346, 1366
Eam1104I CTCTTC 1 cut(s) 1107
EarI CTCTTC 1 cut(s) 1107
EciI GGCGGA 1 cut(s) 909
Eco130I CCWWGG 1 cut(s) 371
Eco147I AGGCCT 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 513
Eco88I CYCGRG 1 cut(s) 639
Eco91I GGTNACC 1 cut(s) 467
EcoO65I GGTNACC 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 541
EcoT14I CCWWGG 1 cut(s) 371
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 371
FauI CCCGC 1 cut(s) 259
FbaI TGATCA 1 cut(s) 1366
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 7 cut(s) 176, 570, 573, 582, 765, 1182, 1388
FokI GGATG 8 cut(s) 82, 134, 758, 927, 929, 932, 1052, 1436
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 7 cut(s) 176, 570, 573, 582, 765, 1182, 1388
FspBI CTAG 7 cut(s) 21, 89, 227, 423, 429, 1230, 1415
GluI GCNGC 7 cut(s) 176, 570, 573, 582, 765, 1182, 1388
GsuI CTGGAG 1 cut(s) 1209
HaeIII GGCC 4 cut(s) 225, 490, 873, 1016
HapII CCGG 1 cut(s) 249
HinfI GANTC 5 cut(s) 206, 790, 842, 1069, 1439
HpaII CCGG 1 cut(s) 249
HphI GGTGA 8 cut(s) 281, 295, 467, 479, 1006, 1064, 1331, 1512
Hpy166II GTNNAC 2 cut(s) 886, 1510
Hpy188I TCNGA 5 cut(s) 246, 340, 357, 610, 1465
Hpy188III TCNNGA 2 cut(s) 1421, 1443
Hpy8I GTNNAC 2 cut(s) 886, 1510
Hpy99I CGWCG 1 cut(s) 1013
HpyAV CCTTC 5 cut(s) 59, 146, 863, 1033, 1368
HpyCH4III ACNGT 2 cut(s) 695, 725
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 7 cut(s) 569, 581, 923, 1126, 1184, 1387, 1487
HpyF10VI GCNNNNNNNGC 3 cut(s) 578, 581, 929
HpyF3I CTNAG 2 cut(s) 354, 857
HpySE526I ACGT 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 1366
Kzo9I GATC 5 cut(s) 161, 241, 457, 1346, 1366
LmnI GCTCC 5 cut(s) 707, 761, 1058, 1083, 1465
Lsp1109I GCAGC 5 cut(s) 581, 593, 776, 1168, 1399
LweI GCATC 5 cut(s) 112, 253, 817, 910, 1474
MaeI CTAG 7 cut(s) 21, 89, 227, 423, 429, 1230, 1415
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 8 cut(s) 254, 269, 467, 526, 725, 901, 1118, 1219
MalI GATC 5 cut(s) 163, 243, 459, 1348, 1368
MboI GATC 5 cut(s) 161, 241, 457, 1346, 1366
MboII GAAGA 7 cut(s) 74, 506, 579, 1094, 1287, 1342, 1448
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 2 cut(s) 355, 1065
MlyI GAGTC 2 cut(s) 799, 851
MmeI TCCRAC 1 cut(s) 1387
MnlI CCTC 5 cut(s) 192, 519, 1006, 1116, 1347
MseI TTAA 8 cut(s) 33, 44, 234, 285, 414, 732, 939, 981
MslI CAYNNNNRTG 1 cut(s) 311
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 2 cut(s) 250, 543
MvaI CCWGG 1 cut(s) 543
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 3 cut(s) 578, 581, 929
NciI CCSGG 1 cut(s) 250
NdeII GATC 5 cut(s) 161, 241, 457, 1346, 1366
NlaIV GGNNCC 1 cut(s) 1467
NmuCI GTSAC 4 cut(s) 269, 467, 725, 1219
NspI RCATGY 5 cut(s) 370, 714, 1126, 1294, 1387
NspV TTCGAA 1 cut(s) 1434
PaeI GCATGC 1 cut(s) 714
PaqCI CACCTGC 1 cut(s) 1083
PceI AGGCCT 1 cut(s) 490
PfeI GAWTC 3 cut(s) 206, 1069, 1439
PflMI CCANNNNNTGG 1 cut(s) 393
PkrI GCNGC 7 cut(s) 177, 571, 574, 583, 766, 1183, 1389
PleI GAGTC 2 cut(s) 798, 850
PpsI GAGTC 2 cut(s) 798, 850
Ppu21I YACGTR 1 cut(s) 218
PsiI TTATAA 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 541
PspEI GGTNACC 1 cut(s) 467
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 1 cut(s) 1467
PstI CTGCAG 1 cut(s) 571
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 2 cut(s) 1269, 1315
RsaNI GTAC 2 cut(s) 1268, 1314
RseI CAYNNNNRTG 1 cut(s) 311
SaqAI TTAA 8 cut(s) 33, 44, 234, 285, 414, 732, 939, 981
SatI GCNGC 7 cut(s) 176, 570, 573, 582, 765, 1182, 1388
Sau3AI GATC 5 cut(s) 161, 241, 457, 1346, 1366
SchI GAGTC 2 cut(s) 799, 851
ScrFI CCNGG 2 cut(s) 250, 543
SduI GDGCHC 2 cut(s) 355, 1065
SfaNI GCATC 5 cut(s) 112, 253, 817, 910, 1474
SfcI CTRYAG 1 cut(s) 567
SfuI TTCGAA 1 cut(s) 1434
SmiMI CAYNNNNRTG 1 cut(s) 311
SpeI ACTAGT 2 cut(s) 88, 422
SphI GCATGC 1 cut(s) 714
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 7 cut(s) 101, 119, 176, 266, 573, 894, 1037
SspI AATATT 1 cut(s) 31
SspMI CTAG 7 cut(s) 21, 89, 227, 423, 429, 1230, 1415
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 2 cut(s) 248, 541
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 2 cut(s) 695, 725
TaiI ACGT 1 cut(s) 220
TaqI TCGA 5 cut(s) 511, 1011, 1361, 1434, 1444
TatI WGTACW 1 cut(s) 1267
TauI GCSGC 2 cut(s) 178, 575
TfiI GAWTC 3 cut(s) 206, 1069, 1439
Tru1I TTAA 8 cut(s) 33, 44, 234, 285, 414, 732, 939, 981
Tru9I TTAA 8 cut(s) 33, 44, 234, 285, 414, 732, 939, 981
TscAI CASTG 1 cut(s) 1356
TseFI GTSAC 4 cut(s) 269, 467, 725, 1219
TseI GCWGC 5 cut(s) 569, 581, 764, 1181, 1387
Tsp45I GTSAC 4 cut(s) 269, 467, 725, 1219
TspDTI ATGAA 4 cut(s) 762, 848, 1015, 1190
TspGWI ACGGA 1 cut(s) 1359
TspRI CASTG 1 cut(s) 1356
Van91I CCANNNNNTGG 1 cut(s) 393
XapI RAATTY 6 cut(s) 281, 334, 659, 835, 908, 1190
XceI RCATGY 5 cut(s) 370, 714, 1126, 1294, 1387
XmiI GTMKAC 1 cut(s) 885
XspI CTAG 7 cut(s) 21, 89, 227, 423, 429, 1230, 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.