Rorug07G0215300

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000663
Physical Location & Seq
Reverse (-)
19102173 .. 19105149
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0215300.1

Sequence Viewer

Length: 1200 bp
ATGGGTGGTGACTCTCCAACCTTTGAGATGATAGATGGTCGCACTTCTGATAATGACTGTGATAAGGAAGAGAAGGGAAAGAGACTATGGAAGAAAGTAAAATATCAGCTTATTGAATACCATTCATTGCCTATGTTCTTAAGGGACAATGAGTTCATTCTGGGTTATTACCGATCAGAATGGCCATTGAAGCAAGTGTTTCTAAGCATGTTCTCTATCCATAATGAAACTCTTAATGTCTGGACGCATTTGATTGGCTTCTTCCTTTTCCTTTTCCTCACCATTTACACAGCAACAAGAGCTCCAGACCTCATGGATCTTCGTTACCTACAACGTTTGCCTGACATGATTAGAAGTGCTGATTTCCACAAAATTCATCCAGAACTGTTGAATTGCCTCCCTTCATTACCTAACCTGTCTGATTTTAAGACATCTTTGCCATCTATGGATTTTCTTTCTTCAGTTTCAGGGAATCTAGTGGAACTTGTCACCAATTGCTTGCCTGAGCGATTATCCCATTCCTACCAAACCGAGAATTCTGTTCCGCACGGAGTAACAGATGATGTGATGAACATGGTTGCTCCCCTTATGGTTCGACCAATTACAAGGTGGCCTTTTTTTGTCTTCCTAGGTGGAGCCATGTTCTGCTTGCTAGCCAGTAGCGCATGCCACCTCCTTGCTTGCCATTCAGCACGCCTTTCCTACATCGTTCTAAGATGTGACTATGCAGGCATCGCTGCGCTCATTGCAACCTCCTTTTACCCTCCCGTTTACTATTCCTTCATGTGCAACCCCTTCTTTTGCAATCTGTACTTGGGATTCATAACAATTCTTGGTATTGCCACCATGGCTTTTTCCCTCCTTCCAATGTTTCAAGGACCCAAGTTCCGCAGTACTCGTGCCTCTCTCTTCTTTGGGATGGGCGTGTCTGGTGTGGTTCCTTTAGTTCATAAGCTTATACTGTTCCGGAATCGGCCTGAGGTCCTCCACACTACTTGTTATGAGGTGTTAATGGGAGTCCTCTATGGATTGGGCGCGGTGATATATGCTACAAGGATACCGGAGCGGTGGAGACCAGGAAAGTTTGACATTGCTGGCCACAGTCACCAACTTTTTCATATATTGGTTGTGGCAGGAGCTTACACACATTATCGAGCAGGACTCGTCTATCTAAGATGGCGGGACTTACAGGGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

399

Amino Acids

45.6

Weight (kDa)

8.42

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HlyIII PF03006 71 - 98 2.5e-06 Haemolysin-III related
HlyIII PF03006 199 - 384 2.7e-54 Haemolysin-III related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1066
AccII CGCG 1 cut(s) 1037
AccIII TCCGGA 1 cut(s) 966
AciI CCGC 5 cut(s) 545, 889, 1037, 1066, 1180
AclI AACGTT 1 cut(s) 334
AclWI GGATC 1 cut(s) 324
AcoI YGGCCR 2 cut(s) 182, 1096
AcsI RAATTY 2 cut(s) 372, 535
AcuI CTGAAG 1 cut(s) 444
AfaI GTAC 2 cut(s) 812, 895
AflII CTTAAG 1 cut(s) 139
AgsI TTSAA 4 cut(s) 116, 190, 391, 875
AjnI CCWGG 1 cut(s) 1075
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 4 cut(s) 109, 302, 955, 1139
AluI AGCT 4 cut(s) 109, 302, 955, 1139
Alw21I GWGCWC 1 cut(s) 304
Alw26I GTCTC 2 cut(s) 76, 1066
AlwI GGATC 1 cut(s) 324
Aor13HI TCCGGA 1 cut(s) 966
AoxI GGCC 4 cut(s) 182, 611, 974, 1096
ApeKI GCWGC 1 cut(s) 737
ApoI RAATTY 2 cut(s) 372, 535
AspA2I CCTAGG 1 cut(s) 628
AspLEI GCGC 3 cut(s) 665, 742, 1037
AspS9I GGNCC 2 cut(s) 878, 982
AsuHPI GGTGA 5 cut(s) 20, 271, 481, 1051, 1097
AsuNHI GCTAGC 1 cut(s) 652
AvaII GGWCC 2 cut(s) 878, 982
AvrII CCTAGG 1 cut(s) 628
AxyI CCTNAGG 1 cut(s) 978
BalI TGGCCA 2 cut(s) 184, 1098
BanII GRGCYC 1 cut(s) 304
BauI CACGAG 1 cut(s) 897
BbsI GAAGAC 1 cut(s) 616
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 724
BccI CCATC 4 cut(s) 29, 448, 913, 1170
BciT130I CCWGG 1 cut(s) 1077
BciVI GTATCC 1 cut(s) 1050
BcoDI GTCTC 2 cut(s) 76, 1066
BfaI CTAG 3 cut(s) 476, 629, 653
BfrI CTTAAG 1 cut(s) 139
BfuI GTATCC 1 cut(s) 1050
BglI GCCNNNNNGGC 1 cut(s) 848
BisI GCNGC 1 cut(s) 738
BlnI CCTAGG 1 cut(s) 628
BlsI GCNGC 1 cut(s) 739
BmcAI AGTACT 1 cut(s) 895
Bme1390I CCNGG 1 cut(s) 1077
Bme18I GGWCC 2 cut(s) 878, 982
BmgT120I GGNCC 2 cut(s) 878, 982
BmiI GGNNCC 3 cut(s) 637, 880, 939
BmrFI CCNGG 1 cut(s) 1077
BmsI GCATC 1 cut(s) 741
BmtI GCTAGC 1 cut(s) 656
BpiI GAAGAC 1 cut(s) 616
BplI GAGNNNNNCTC 2 cut(s) 1146, 1178
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 504
BsaI GGTCTC 1 cut(s) 1066
BsaJI CCNNGG 2 cut(s) 628, 846
BsaWI WCCGGW 2 cut(s) 966, 1060
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bse1I ACTGG 1 cut(s) 657
Bse21I CCTNAGG 1 cut(s) 978
Bse3DI GCAATG 3 cut(s) 125, 744, 1089
BseAI TCCGGA 1 cut(s) 966
BseBI CCWGG 1 cut(s) 1077
BseDI CCNNGG 2 cut(s) 628, 846
BseGI GGATG 2 cut(s) 376, 924
BseMI GCAATG 3 cut(s) 125, 744, 1089
BseMII CTCAG 2 cut(s) 495, 969
BseNI ACTGG 1 cut(s) 657
BseXI GCAGC 1 cut(s) 724
Bsh1236I CGCG 1 cut(s) 1037
BshFI GGCC 4 cut(s) 184, 613, 976, 1098
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 2 cut(s) 967, 1061
BslFI GGGAC 2 cut(s) 158, 1196
BsmAI GTCTC 2 cut(s) 76, 1066
BsmFI GGGAC 2 cut(s) 158, 1196
BsnI GGCC 4 cut(s) 184, 613, 976, 1098
Bso31I GGTCTC 1 cut(s) 1066
Bsp1286I GDGCHC 1 cut(s) 304
Bsp13I TCCGGA 1 cut(s) 966
Bsp143I GATC 2 cut(s) 173, 316
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 5 cut(s) 545, 889, 1037, 1066, 1180
BspANI GGCC 4 cut(s) 184, 613, 976, 1098
BspCNI CTCAG 2 cut(s) 496, 970
BspEI TCCGGA 1 cut(s) 966
BspFNI CGCG 1 cut(s) 1037
BspLI GGNNCC 3 cut(s) 637, 880, 939
BspOI GCTAGC 1 cut(s) 656
BspPI GGATC 1 cut(s) 324
BspTI CTTAAG 1 cut(s) 139
BspTNI GGTCTC 1 cut(s) 1066
BsrBI CCGCTC 1 cut(s) 1066
BsrDI GCAATG 3 cut(s) 125, 744, 1089
BsrI ACTGG 1 cut(s) 657
BssECI CCNNGG 2 cut(s) 628, 846
BssMI GATC 2 cut(s) 173, 316
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 2 cut(s) 628, 846
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 1 cut(s) 1077
Bst4CI ACNGT 4 cut(s) 59, 387, 963, 1103
Bst6I CTCTTC 2 cut(s) 63, 914
BstAFI CTTAAG 1 cut(s) 139
BstC8I GCNNGC 8 cut(s) 500, 650, 654, 667, 682, 694, 730, 1096
BstDEI CTNAG 5 cut(s) 203, 504, 713, 978, 1172
BstDSI CCRYGG 1 cut(s) 846
BstF5I GGATG 2 cut(s) 376, 924
BstFNI CGCG 1 cut(s) 1037
BstHHI GCGC 3 cut(s) 665, 742, 1037
BstKTI GATC 2 cut(s) 176, 319
BstMAI GTCTC 2 cut(s) 76, 1066
BstMBI GATC 2 cut(s) 173, 316
BstMWI GCNNNNNNNGC 6 cut(s) 190, 299, 662, 734, 746, 848
BstNI CCWGG 1 cut(s) 1077
BstNSI RCATGY 2 cut(s) 211, 669
BstSCI CCNGG 1 cut(s) 1075
BstUI CGCG 1 cut(s) 1037
BstV1I GCAGC 1 cut(s) 724
BstV2I GAAGAC 1 cut(s) 616
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
Bsu36I CCTNAGG 1 cut(s) 978
BsuI GTATCC 1 cut(s) 1050
BsuRI GGCC 4 cut(s) 184, 613, 976, 1098
BtgI CCRYGG 1 cut(s) 846
BtgZI GCGATG 1 cut(s) 718
BtsCI GGATG 2 cut(s) 376, 924
Cac8I GCNNGC 8 cut(s) 500, 650, 654, 667, 682, 694, 730, 1096
CfoI GCGC 3 cut(s) 665, 742, 1037
Cfr13I GGNCC 2 cut(s) 878, 982
CseI GACGC 1 cut(s) 253
Csp6I GTAC 2 cut(s) 811, 894
CspCI CAANNNNNGTGG 2 cut(s) 977, 1012
CviAII CATG 8 cut(s) 208, 313, 346, 574, 640, 666, 784, 847
CviQI GTAC 2 cut(s) 811, 894
DdeI CTNAG 5 cut(s) 203, 504, 713, 978, 1172
DpnI GATC 2 cut(s) 175, 318
DpnII GATC 2 cut(s) 173, 316
EaeI YGGCCR 2 cut(s) 182, 1096
Eam1104I CTCTTC 2 cut(s) 63, 914
EarI CTCTTC 2 cut(s) 63, 914
Ecl136II GAGCTC 1 cut(s) 302
Eco130I CCWWGG 2 cut(s) 628, 846
Eco24I GRGCYC 1 cut(s) 304
Eco31I GGTCTC 1 cut(s) 1066
Eco47I GGWCC 2 cut(s) 878, 982
Eco53kI GAGCTC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 444
Eco81I CCTNAGG 1 cut(s) 978
EcoICRI GAGCTC 1 cut(s) 302
EcoO109I RGGNCCY 2 cut(s) 878, 982
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 1075
EcoT14I CCWWGG 2 cut(s) 628, 846
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 2 cut(s) 628, 846
FaeI CATG 8 cut(s) 211, 316, 349, 577, 643, 669, 787, 850
FalI AAGNNNNNCTT 2 cut(s) 598, 630
FaqI GGGAC 2 cut(s) 158, 1196
FatI CATG 8 cut(s) 207, 312, 345, 573, 639, 665, 783, 846
FauI CCCGC 1 cut(s) 1173
Fnu4HI GCNGC 1 cut(s) 738
FokI GGATG 2 cut(s) 363, 931
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 1 cut(s) 738
FspBI CTAG 3 cut(s) 476, 629, 653
GlaI GCGC 3 cut(s) 664, 741, 1036
GluI GCNGC 1 cut(s) 738
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 4 cut(s) 184, 613, 976, 1098
HapII CCGG 2 cut(s) 967, 1061
HgaI GACGC 1 cut(s) 253
HhaI GCGC 3 cut(s) 665, 742, 1037
Hin1II CATG 8 cut(s) 211, 316, 349, 577, 643, 669, 787, 850
Hin6I GCGC 3 cut(s) 663, 740, 1035
HinP1I GCGC 3 cut(s) 663, 740, 1035
HindIII AAGCTT 1 cut(s) 953
HinfI GANTC 6 cut(s) 11, 472, 819, 970, 1017, 1161
HpaII CCGG 2 cut(s) 967, 1061
HphI GGTGA 5 cut(s) 20, 271, 481, 1051, 1097
Hpy166II GTNNAC 1 cut(s) 772
Hpy188I TCNGA 3 cut(s) 49, 178, 421
Hpy188III TCNNGA 4 cut(s) 241, 305, 380, 967
Hpy8I GTNNAC 1 cut(s) 772
HpyAV CCTTC 5 cut(s) 67, 411, 790, 805, 872
HpyCH4III ACNGT 4 cut(s) 59, 387, 963, 1103
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 4 cut(s) 728, 749, 789, 804
HpyF10VI GCNNNNNNNGC 6 cut(s) 190, 299, 662, 734, 746, 848
HpyF3I CTNAG 5 cut(s) 203, 504, 713, 978, 1172
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 8 cut(s) 211, 316, 349, 577, 643, 669, 787, 850
HspAI GCGC 3 cut(s) 663, 740, 1035
Kpn2I TCCGGA 1 cut(s) 966
Kzo9I GATC 2 cut(s) 173, 316
LmnI GCTCC 5 cut(s) 307, 586, 635, 1063, 1136
Lsp1109I GCAGC 1 cut(s) 724
LweI GCATC 1 cut(s) 741
MaeI CTAG 3 cut(s) 476, 629, 653
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 6 cut(s) 8, 323, 487, 553, 719, 1103
MalI GATC 2 cut(s) 175, 318
MbiI CCGCTC 1 cut(s) 1066
MboI GATC 2 cut(s) 173, 316
MboII GAAGA 7 cut(s) 80, 103, 253, 311, 450, 616, 901
MfeI CAATTG 1 cut(s) 493
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 304
MlsI TGGCCA 2 cut(s) 184, 1098
MluCI AATT 6 cut(s) 372, 391, 493, 535, 600, 828
MluNI TGGCCA 2 cut(s) 184, 1098
MlyI GAGTC 3 cut(s) 5, 1026, 1155
MmeI TCCRAC 1 cut(s) 41
Mox20I TGGCCA 2 cut(s) 184, 1098
MroI TCCGGA 1 cut(s) 966
MscI TGGCCA 2 cut(s) 184, 1098
MseI TTAA 4 cut(s) 140, 234, 426, 1010
Msp20I TGGCCA 2 cut(s) 184, 1098
MspCI CTTAAG 1 cut(s) 139
MspI CCGG 2 cut(s) 967, 1061
MspR9I CCNGG 1 cut(s) 1077
MunI CAATTG 1 cut(s) 493
MvaI CCWGG 1 cut(s) 1077
MvnI CGCG 1 cut(s) 1037
MwoI GCNNNNNNNGC 6 cut(s) 190, 299, 662, 734, 746, 848
NcoI CCATGG 1 cut(s) 846
NdeII GATC 2 cut(s) 173, 316
NheI GCTAGC 1 cut(s) 652
NlaIII CATG 8 cut(s) 211, 316, 349, 577, 643, 669, 787, 850
NlaIV GGNNCC 3 cut(s) 637, 880, 939
NmuCI GTSAC 4 cut(s) 8, 487, 719, 1103
NspI RCATGY 2 cut(s) 211, 669
PaeI GCATGC 1 cut(s) 669
PfeI GAWTC 3 cut(s) 472, 819, 970
PkrI GCNGC 1 cut(s) 739
PleI GAGTC 3 cut(s) 5, 1025, 1155
PpsI GAGTC 3 cut(s) 5, 1025, 1155
PpuMI RGGWCCY 2 cut(s) 878, 982
Psp124BI GAGCTC 1 cut(s) 304
Psp1406I AACGTT 1 cut(s) 334
Psp5II RGGWCCY 2 cut(s) 878, 982
Psp6I CCWGG 1 cut(s) 1075
PspGI CCWGG 1 cut(s) 1075
PspN4I GGNNCC 3 cut(s) 637, 880, 939
PspPI GGNCC 2 cut(s) 878, 982
PspPPI RGGWCCY 2 cut(s) 878, 982
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 2 cut(s) 812, 895
RsaNI GTAC 2 cut(s) 811, 894
SacI GAGCTC 1 cut(s) 304
SaqAI TTAA 4 cut(s) 140, 234, 426, 1010
SatI GCNGC 1 cut(s) 738
Sau3AI GATC 2 cut(s) 173, 316
Sau96I GGNCC 2 cut(s) 878, 982
ScaI AGTACT 1 cut(s) 895
SchI GAGTC 3 cut(s) 5, 1026, 1155
ScrFI CCNGG 1 cut(s) 1077
SduI GDGCHC 1 cut(s) 304
SfaNI GCATC 1 cut(s) 741
SinI GGWCC 2 cut(s) 878, 982
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
SphI GCATGC 1 cut(s) 669
Sse9I AATT 6 cut(s) 372, 391, 493, 535, 600, 828
SsiI CCGC 5 cut(s) 545, 889, 1037, 1066, 1180
SspMI CTAG 3 cut(s) 476, 629, 653
SstI GAGCTC 1 cut(s) 304
StyD4I CCNGG 1 cut(s) 1075
StyI CCWWGG 2 cut(s) 628, 846
TaaI ACNGT 4 cut(s) 59, 387, 963, 1103
TaiI ACGT 1 cut(s) 337
TaqI TCGA 2 cut(s) 595, 1153
TasI AATT 6 cut(s) 372, 391, 493, 535, 600, 828
TatI WGTACW 2 cut(s) 810, 893
TfiI GAWTC 3 cut(s) 472, 819, 970
Tru1I TTAA 4 cut(s) 140, 234, 426, 1010
Tru9I TTAA 4 cut(s) 140, 234, 426, 1010
TseFI GTSAC 4 cut(s) 8, 487, 719, 1103
TseI GCWGC 1 cut(s) 737
Tsp45I GTSAC 4 cut(s) 8, 487, 719, 1103
TspGWI ACGGA 1 cut(s) 564
Vha464I CTTAAG 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 878, 982
XapI RAATTY 2 cut(s) 372, 535
XceI RCATGY 2 cut(s) 211, 669
XcmI CCANNNNNNNNNTGG 1 cut(s) 606
XmaJI CCTAGG 1 cut(s) 628
XspI CTAG 3 cut(s) 476, 629, 653
ZrmI AGTACT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.