Rh1AG395200

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000663
Physical Location & Seq
Forward (+)
62342583 .. 62343949
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG395200.1

Sequence Viewer

Length: 738 bp
ATGTGTACCAATTTCCTTCTGTTAAGGCTGTTGATAGCAGCAGGGTTGTTGGCAATGGCAACCGCCGCAAAAGTGACTCCACATAACTACGCAGATGCTCTGTCGAAGTCCATTTTATTTTTTGAAGGCCAGAGATCTGGCAAGTTACCGAATTCTCAACGAATGAATTGGAGGAAAGATTCTGCCCTACATGATGGCAGCGATGTTCATAGGGATCTGGTGGGGGGTTACCATGATGCTGGTGACAATGTGAAGTTCAACTTTCCCATGGCCTTCACAACAACATTGTTGTCATGGAGCGTTCTAGAGTTTGGAGATTTGATGGGATCTGAACTTCAACATGCTTTGGAAGCTATAAGATGGGCAACTGATTATTTTCTTAAAGCCACAAATGATCCTGGTTCAGTAGTTGGCGTAGTACGCGACCCCAACGGAGATCATGCTTGTTGGCAAAAGCCTGAAGACGTGGATACCCCAAGAACCACATATGTGGTAACTAAAGAAAAACCAGGTTCAGAAGTTTCTGCGGAGATTGCAGCCATGCTTGCAGCTTCCTCTATAGTGTTTAACCAATCAGACAAAGCCTACTCTGACTCGCTTCTTAAGCGAGCCATCCAAGTGTTTCAATTTGCAGATCAGTATCAGGGATCTTACAATACTAGTATTGGAGACGGAGCCTGCCCATTTTACTGCGATTTTAGTGGCTACATGGATGAATTGATTTGGGGAGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

245

Amino Acids

26.95

Weight (kDa)

5.05

Isoelectric Point (pI)

29.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 244 9.1e-85 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 423
AciI CCGC 3 cut(s) 63, 66, 527
AclWI GGATC 4 cut(s) 222, 334, 389, 655
AcsI RAATTY 1 cut(s) 151
AcuI CTGAAG 1 cut(s) 480
AfaI GTAC 2 cut(s) 7, 420
AflII CTTAAG 1 cut(s) 602
AgsI TTSAA 4 cut(s) 125, 259, 338, 626
AhlI ACTAGT 1 cut(s) 659
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 2 cut(s) 397, 508
AleI CACNNNNGTG 1 cut(s) 488
AluBI AGCT 3 cut(s) 353, 551, 731
AluI AGCT 3 cut(s) 353, 551, 731
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 4 cut(s) 222, 334, 389, 655
AoxI GGCC 2 cut(s) 127, 270
ApeKI GCWGC 4 cut(s) 38, 198, 536, 548
ApoI RAATTY 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 4 cut(s) 50, 210, 548, 560
BccI CCATC 4 cut(s) 188, 316, 354, 620
BciT130I CCWGG 2 cut(s) 399, 510
BciVI GTATCC 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 663
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 2 cut(s) 305, 660
BfmI CTRYAG 1 cut(s) 558
BfrI CTTAAG 1 cut(s) 602
BfuI GTATCC 1 cut(s) 463
BglII AGATCT 1 cut(s) 134
BisI GCNGC 5 cut(s) 39, 66, 199, 537, 549
BlsI GCNGC 5 cut(s) 40, 67, 200, 538, 550
Bme1390I CCNGG 2 cut(s) 399, 510
BmgBI CACGTC 1 cut(s) 466
BmiI GGNNCC 1 cut(s) 676
BmrFI CCNGG 2 cut(s) 399, 510
BmsI GCATC 2 cut(s) 85, 226
BpiI GAAGAC 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 639
BsaJI CCNNGG 1 cut(s) 267
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 639
BseBI CCWGG 2 cut(s) 399, 510
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 2 cut(s) 612, 718
BseJI GATNNNNATC 1 cut(s) 639
BseMI GCAATG 1 cut(s) 60
BseXI GCAGC 4 cut(s) 50, 210, 548, 560
Bsh1236I CGCG 1 cut(s) 423
BshFI GGCC 2 cut(s) 129, 272
BsmAI GTCTC 1 cut(s) 663
BsmBI CGTCTC 1 cut(s) 663
BsnI GGCC 2 cut(s) 129, 272
Bsp143I GATC 7 cut(s) 134, 214, 326, 394, 436, 634, 647
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 3 cut(s) 63, 66, 527
BspANI GGCC 2 cut(s) 129, 272
BspFNI CGCG 1 cut(s) 423
BspLI GGNNCC 1 cut(s) 676
BspPI GGATC 4 cut(s) 222, 334, 389, 655
BspTI CTTAAG 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 7 cut(s) 134, 214, 326, 394, 436, 634, 647
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 2 cut(s) 399, 510
BstAFI CTTAAG 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 546, 609, 679
BstDSI CCRYGG 1 cut(s) 267
BstEII GGTNACC 1 cut(s) 227
BstF5I GGATG 2 cut(s) 612, 718
BstFNI CGCG 1 cut(s) 423
BstKTI GATC 7 cut(s) 137, 217, 329, 397, 439, 637, 650
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 7 cut(s) 134, 214, 326, 394, 436, 634, 647
BstMWI GCNNNNNNNGC 6 cut(s) 65, 350, 420, 533, 545, 604
BstNI CCWGG 2 cut(s) 399, 510
BstNSI RCATGY 1 cut(s) 344
BstPI GGTNACC 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 397, 508
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 1 cut(s) 423
BstV1I GCAGC 4 cut(s) 50, 210, 548, 560
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 4 cut(s) 134, 214, 326, 647
BstXI CCANNNNNNTGG 3 cut(s) 137, 239, 490
BstYI RGATCY 4 cut(s) 134, 214, 326, 647
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 2 cut(s) 129, 272
BtgI CCRYGG 1 cut(s) 267
BtgZI GCGATG 1 cut(s) 216
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 2 cut(s) 612, 718
Cac8I GCNNGC 3 cut(s) 546, 609, 679
CsiI ACCWGGT 1 cut(s) 508
Csp6I GTAC 2 cut(s) 6, 419
CviAII CATG 8 cut(s) 191, 233, 268, 294, 341, 440, 541, 709
CviQI GTAC 2 cut(s) 6, 419
DpnI GATC 7 cut(s) 136, 216, 328, 396, 438, 636, 649
DpnII GATC 7 cut(s) 134, 214, 326, 394, 436, 634, 647
Eco130I CCWWGG 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 480
Eco91I GGTNACC 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 227
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 397, 508
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
Esp3I CGTCTC 1 cut(s) 663
FaeI CATG 8 cut(s) 194, 236, 271, 297, 344, 443, 544, 712
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FatI CATG 8 cut(s) 190, 232, 267, 293, 340, 439, 540, 708
FauNDI CATATG 1 cut(s) 487
Fnu4HI GCNGC 5 cut(s) 39, 66, 199, 537, 549
FokI GGATG 2 cut(s) 599, 725
Fsp4HI GCNGC 5 cut(s) 39, 66, 199, 537, 549
FspBI CTAG 2 cut(s) 305, 660
GluI GCNGC 5 cut(s) 39, 66, 199, 537, 549
HaeIII GGCC 2 cut(s) 129, 272
Hin1II CATG 8 cut(s) 194, 236, 271, 297, 344, 443, 544, 712
HinfI GANTC 3 cut(s) 76, 179, 593
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 4 cut(s) 331, 517, 577, 592
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 3 cut(s) 26, 119, 283
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 3 cut(s) 536, 548, 632
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 350, 420, 533, 545, 604
HpySE526I ACGT 1 cut(s) 465
Hsp92II CATG 8 cut(s) 194, 236, 271, 297, 344, 443, 544, 712
Kzo9I GATC 7 cut(s) 134, 214, 326, 394, 436, 634, 647
LmnI GCTCC 3 cut(s) 297, 674, 728
Lsp1109I GCAGC 4 cut(s) 50, 210, 548, 560
LweI GCATC 2 cut(s) 85, 226
MabI ACCWGGT 1 cut(s) 508
MaeI CTAG 2 cut(s) 305, 660
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 5 cut(s) 73, 144, 227, 242, 493
MalI GATC 7 cut(s) 136, 216, 328, 396, 438, 636, 649
MboI GATC 7 cut(s) 134, 214, 326, 394, 436, 634, 647
MboII GAAGA 1 cut(s) 473
MflI RGATCY 4 cut(s) 134, 214, 326, 647
MluCI AATT 5 cut(s) 10, 151, 166, 626, 716
MlyI GAGTC 2 cut(s) 70, 587
MnlI CCTC 2 cut(s) 165, 565
MseI TTAA 4 cut(s) 23, 381, 567, 603
MslI CAYNNNNRTG 2 cut(s) 488, 617
MspCI CTTAAG 1 cut(s) 602
MspR9I CCNGG 2 cut(s) 399, 510
MvaI CCWGG 2 cut(s) 399, 510
MvnI CGCG 1 cut(s) 423
MwoI GCNNNNNNNGC 6 cut(s) 65, 350, 420, 533, 545, 604
NcoI CCATGG 1 cut(s) 267
NdeI CATATG 1 cut(s) 487
NdeII GATC 7 cut(s) 134, 214, 326, 394, 436, 634, 647
NlaIII CATG 8 cut(s) 194, 236, 271, 297, 344, 443, 544, 712
NlaIV GGNNCC 1 cut(s) 676
NmuCI GTSAC 2 cut(s) 73, 242
NspI RCATGY 1 cut(s) 344
OliI CACNNNNGTG 1 cut(s) 488
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 5 cut(s) 40, 67, 200, 538, 550
PleI GAGTC 2 cut(s) 70, 587
PpsI GAGTC 2 cut(s) 70, 587
Psp6I CCWGG 2 cut(s) 397, 508
PspEI GGTNACC 1 cut(s) 227
PspGI CCWGG 2 cut(s) 397, 508
PspN4I GGNNCC 1 cut(s) 676
PsuI RGATCY 4 cut(s) 134, 214, 326, 647
RsaI GTAC 2 cut(s) 7, 420
RsaNI GTAC 2 cut(s) 6, 419
RseI CAYNNNNRTG 2 cut(s) 488, 617
SaqAI TTAA 4 cut(s) 23, 381, 567, 603
SatI GCNGC 5 cut(s) 39, 66, 199, 537, 549
Sau3AI GATC 7 cut(s) 134, 214, 326, 394, 436, 634, 647
SchI GAGTC 2 cut(s) 70, 587
ScrFI CCNGG 2 cut(s) 399, 510
SetI ASST 5 cut(s) 355, 468, 514, 553, 733
SexAI ACCWGGT 1 cut(s) 508
SfaNI GCATC 2 cut(s) 85, 226
SfcI CTRYAG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 488, 617
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SpeI ACTAGT 1 cut(s) 659
Sse9I AATT 5 cut(s) 10, 151, 166, 626, 716
SsiI CCGC 3 cut(s) 63, 66, 527
SspMI CTAG 2 cut(s) 305, 660
StyD4I CCNGG 2 cut(s) 397, 508
StyI CCWWGG 1 cut(s) 267
TaiI ACGT 1 cut(s) 468
TaqI TCGA 1 cut(s) 104
TasI AATT 5 cut(s) 10, 151, 166, 626, 716
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 4 cut(s) 23, 381, 567, 603
Tru9I TTAA 4 cut(s) 23, 381, 567, 603
TseFI GTSAC 2 cut(s) 73, 242
TseI GCWGC 4 cut(s) 38, 198, 536, 548
Tsp45I GTSAC 2 cut(s) 73, 242
TspDTI ATGAA 4 cut(s) 179, 197, 723, 729
TspGWI ACGGA 2 cut(s) 447, 687
Vha464I CTTAAG 1 cut(s) 602
XapI RAATTY 1 cut(s) 151
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 1 cut(s) 344
XspI CTAG 2 cut(s) 305, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.