Rroxscaffold_4G00283380

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000663
Physical Location & Seq
Reverse (-)
5303733 .. 5306659
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283380.1

Sequence Viewer

Length: 1317 bp
ATGATGGTGGTGATGGTGGTGAAGGCTTCGCAGGACTATGGGGATGCATTGACGAAGAGCATATTGTTCTTTGAGGGCCAGAGGTCAGGGAAGCTACCCTCTCGTCAGAGAATGACTTGGAGGAAAGATTCTGCACTTCAGGATGGACTTCAGATTGGTGTTGATTTGGTAGGGGGTTACTACGATGCCGGTGACAATGTGAAGTTCAACTTTCCGATGGCTTTCTCGACAACAATGATGGCATGGAGCGTGTTAGAGTTCGGGAAGGACATGGGTGCAGACCTACCCCGTGCACTTGATGCCATTCGATGGGGAACGGACTACTTCCTCAAAGCCACAAGCATTCCTGGCTTTGTGTTTGCCCAAGTTGGTGAACCTTACGGCGACCACAATTGTTGGGAGAGGCCTGAGGACATGGACACCCCAAGAAACCCCTATGCAGTTAGCAAACAGTTCCCAGGTTCTGAAGTCTCAGCAGAGATTGCAGCTGCCCTAGCAGCCTCTTCCATGGTTTTTAAGCATGTTGATTTGGAATACTCTGCAAGGCTTCTCAAAAGGGCTAGAATGGTGTTTGAGTTTGCAGAAACATACCGGGGCTCCTATAATGATGCTCTTGGACCATGGGTTTGTCCATTTTACTGTGATTTTAGTGGCTATGAGGTGGAAGCTTTGCCGAATTTGGATGGGATTCAAAGCACGCAGGCATCAATGTACTGGCTTCCAAGCATCGATATCGGTTCTAGTCCTTTCATTCACAATTCCGACAAGTTCATATGCAGTTTGTTACCTGAATCGCCAACCATATCAGTCTCATATTCTCCAGGTGGCCTTTTATTTAAACCTGGAGGAAGTAATCTGCAGCATACAACAACTCTGTCATTTCTCTTGGTTGTTTATTCTCGTTATCTGAAGCAAGCCAATAGAGTAGTTTACTGTAACAATGTTGTTGCGAGTCCAGCTAGACTTGTTCAAGTTGCAAAAGGCCAGGTGGATTACATACTAGGAAGTAACCCATTGAACATGTCATATATGGTCGGGTATGGCAAAAAGTTTCCTCAGAGAATTCATCATCGGGGGTCATCGTTACCGTCGGTTGGTCAACACCCTCAACAGATTGGTTGCAAAGACGGAAGCGTTTACTTTGACAGCAGTAACCCAAATCCTAATATGCTAACTGGGGCTGTTGTTGGAGGACCAGATATAAAGGATTCATATGAAGATTCTCGAGTGGATTTTGTGCAGTCCGAGCCAACCACATACATCAATGCACCATTTGTAGGCGTCTTGGCTTACTTCAAATCGCATCCATGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

438

Amino Acids

48.46

Weight (kDa)

5.93

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 13 - 220 3.6e-79 Glycosyl hydrolase family 9
Glyco_hydro_9 PF00759 249 - 429 3e-47 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 309
AcsI RAATTY 2 cut(s) 676, 1062
AcuI CTGAAG 4 cut(s) 122, 134, 486, 929
AcyI GRCGYC 1 cut(s) 1281
AfaI GTAC 1 cut(s) 713
AfiI CCNNNNNNNGG 3 cut(s) 309, 1094, 1277
AflIII ACRYGT 1 cut(s) 1020
AgsI TTSAA 5 cut(s) 208, 692, 971, 1018, 1297
AjnI CCWGG 5 cut(s) 346, 457, 820, 841, 984
AluBI AGCT 4 cut(s) 94, 488, 668, 959
AluI AGCT 4 cut(s) 94, 488, 668, 959
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 2 cut(s) 475, 814
Alw44I GTGCAC 1 cut(s) 291
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 1224
AoxI GGCC 4 cut(s) 76, 404, 826, 982
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 4 cut(s) 485, 488, 497, 859
ApoI RAATTY 2 cut(s) 676, 1062
ArsI GACNNNNNNTTYG 2 cut(s) 609, 641
AspS9I GGNCC 4 cut(s) 76, 617, 1193, 1311
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 4 cut(s) 22, 31, 203, 383
AvaI CYCGRG 1 cut(s) 1224
AvaII GGWCC 3 cut(s) 617, 1193, 1311
AxyI CCTNAGG 1 cut(s) 408
BaeGI GKGCMC 1 cut(s) 295
BanII GRGCYC 1 cut(s) 599
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 4 cut(s) 475, 497, 509, 871
BccI CCATC 6 cut(s) 7, 137, 211, 232, 303, 677
BceAI ACGGC 1 cut(s) 397
BcgI CGANNNNNNTGC 2 cut(s) 715, 749
BciT130I CCWGG 5 cut(s) 348, 459, 822, 843, 986
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 2 cut(s) 475, 814
BfaI CTAG 5 cut(s) 494, 561, 741, 960, 1001
BfmI CTRYAG 1 cut(s) 857
BisI GCNGC 4 cut(s) 486, 489, 498, 860
BlsI GCNGC 4 cut(s) 487, 490, 499, 861
Bme1390I CCNGG 6 cut(s) 348, 459, 593, 822, 843, 986
Bme18I GGWCC 3 cut(s) 617, 1193, 1311
BmeT110I CYCGRG 1 cut(s) 1224
BmgT120I GGNCC 4 cut(s) 76, 617, 1193, 1311
BmiI GGNNCC 1 cut(s) 598
BmrFI CCNGG 6 cut(s) 348, 459, 593, 822, 843, 986
BmrI ACTGGG 1 cut(s) 1185
BmsI GCATC 7 cut(s) 34, 175, 289, 598, 713, 735, 1312
BmuI ACTGGG 1 cut(s) 1185
BpmI CTGGAG 2 cut(s) 804, 864
BpuMI CCSGG 1 cut(s) 593
Bsa29I ATCGAT 1 cut(s) 729
BsaHI GRCGYC 1 cut(s) 1281
BsaJI CCNNGG 5 cut(s) 457, 507, 592, 620, 1307
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 1094, 1277
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 2 cut(s) 719, 1180
Bse21I CCTNAGG 1 cut(s) 408
BseBI CCWGG 5 cut(s) 348, 459, 822, 843, 986
BseCI ATCGAT 1 cut(s) 729
BseDI CCNNGG 5 cut(s) 457, 507, 592, 620, 1307
BseGI GGATG 4 cut(s) 49, 148, 688, 1303
BseLI CCNNNNNNNGG 3 cut(s) 309, 1094, 1277
BseMII CTCAG 3 cut(s) 399, 486, 1070
BseNI ACTGG 2 cut(s) 719, 1180
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 4 cut(s) 475, 497, 509, 871
BsgI GTGCAG 3 cut(s) 117, 297, 1259
BshFI GGCC 4 cut(s) 78, 406, 828, 984
BshVI ATCGAT 1 cut(s) 729
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 1 cut(s) 1224
BsiSI CCGG 2 cut(s) 189, 592
BslI CCNNNNNNNGG 3 cut(s) 309, 1094, 1277
BsmAI GTCTC 2 cut(s) 475, 814
BsmI GAATGC 1 cut(s) 342
BsnI GGCC 4 cut(s) 78, 406, 828, 984
BsoBI CYCGRG 1 cut(s) 1224
Bsp1286I GDGCHC 2 cut(s) 295, 599
Bsp19I CCATGG 3 cut(s) 507, 620, 1307
BspANI GGCC 4 cut(s) 78, 406, 828, 984
BspCNI CTCAG 3 cut(s) 400, 485, 1069
BspDI ATCGAT 1 cut(s) 729
BspLI GGNNCC 1 cut(s) 598
BspMAI CTGCAG 1 cut(s) 861
BspQI GCTCTTC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 2 cut(s) 719, 1180
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 5 cut(s) 457, 507, 592, 620, 1307
BssNI GRCGYC 1 cut(s) 1281
BssT1I CCWWGG 3 cut(s) 507, 620, 1307
Bst2UI CCWGG 5 cut(s) 348, 459, 822, 843, 986
Bst4CI ACNGT 4 cut(s) 453, 641, 935, 1089
Bst6I CTCTTC 2 cut(s) 50, 508
BstACI GRCGYC 1 cut(s) 1281
BstAPI GCANNNNNTGC 2 cut(s) 299, 482
BstC8I GCNNGC 3 cut(s) 698, 702, 915
BstDEI CTNAG 3 cut(s) 408, 472, 1056
BstDSI CCRYGG 3 cut(s) 507, 620, 1307
BstF5I GGATG 4 cut(s) 49, 148, 688, 1303
BstMAI GTCTC 2 cut(s) 475, 814
BstMWI GCNNNNNNNGC 7 cut(s) 299, 348, 482, 494, 497, 956, 1246
BstNI CCWGG 5 cut(s) 348, 459, 822, 843, 986
BstNSI RCATGY 2 cut(s) 524, 1024
BstSCI CCNGG 6 cut(s) 346, 457, 591, 820, 841, 984
BstSFI CTRYAG 1 cut(s) 857
BstSLI GKGCMC 1 cut(s) 295
BstV1I GCAGC 4 cut(s) 475, 497, 509, 871
Bsu15I ATCGAT 1 cut(s) 729
Bsu36I CCTNAGG 1 cut(s) 408
BsuRI GGCC 4 cut(s) 78, 406, 828, 984
BsuTUI ATCGAT 1 cut(s) 729
BtgI CCRYGG 3 cut(s) 507, 620, 1307
BtsCI GGATG 4 cut(s) 49, 148, 688, 1303
Cac8I GCNNGC 3 cut(s) 698, 702, 915
CaiI CAGNNNCTG 1 cut(s) 464
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 4 cut(s) 76, 617, 1193, 1311
ClaI ATCGAT 1 cut(s) 729
CseI GACGC 1 cut(s) 1270
Csp6I GTAC 1 cut(s) 712
CspCI CAANNNNNGTGG 2 cut(s) 377, 412
CviAII CATG 8 cut(s) 243, 271, 415, 508, 521, 621, 1021, 1308
CviQI GTAC 1 cut(s) 712
DdeI CTNAG 3 cut(s) 408, 472, 1056
DraI TTTAAA 1 cut(s) 838
Eam1104I CTCTTC 2 cut(s) 50, 508
EarI CTCTTC 2 cut(s) 50, 508
Eco130I CCWWGG 3 cut(s) 507, 620, 1307
Eco147I AGGCCT 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 599
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 3 cut(s) 617, 1193, 1311
Eco57I CTGAAG 4 cut(s) 122, 134, 486, 929
Eco81I CCTNAGG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 1224
EcoRI GAATTC 1 cut(s) 1062
EcoRII CCWGG 5 cut(s) 346, 457, 820, 841, 984
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 3 cut(s) 507, 620, 1307
EcoT22I ATGCAT 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 599
ErhI CCWWGG 3 cut(s) 507, 620, 1307
FaeI CATG 8 cut(s) 246, 274, 418, 511, 524, 624, 1024, 1311
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FatI CATG 8 cut(s) 242, 270, 414, 507, 520, 620, 1020, 1307
FauNDI CATATG 2 cut(s) 773, 1213
Fnu4HI GCNGC 4 cut(s) 486, 489, 498, 860
FokI GGATG 4 cut(s) 56, 155, 695, 1290
FriOI GRGCYC 1 cut(s) 599
Fsp4HI GCNGC 4 cut(s) 486, 489, 498, 860
FspBI CTAG 5 cut(s) 494, 561, 741, 960, 1001
GluI GCNGC 4 cut(s) 486, 489, 498, 860
GsuI CTGGAG 2 cut(s) 804, 864
HaeIII GGCC 4 cut(s) 78, 406, 828, 984
HapII CCGG 2 cut(s) 189, 592
HgaI GACGC 1 cut(s) 1270
Hin1I GRCGYC 1 cut(s) 1281
Hin1II CATG 8 cut(s) 246, 274, 418, 511, 524, 624, 1024, 1311
HincII GTYRAC 1 cut(s) 1100
HindII GTYRAC 1 cut(s) 1100
HindIII AAGCTT 1 cut(s) 666
HinfI GANTC 6 cut(s) 128, 688, 791, 952, 1208, 1220
HpaII CCGG 2 cut(s) 189, 592
HphI GGTGA 4 cut(s) 22, 31, 203, 383
Hpy166II GTNNAC 5 cut(s) 293, 374, 931, 1100, 1138
Hpy188I TCNGA 8 cut(s) 108, 153, 216, 466, 763, 909, 1059, 1246
Hpy188III TCNNGA 4 cut(s) 140, 226, 262, 1224
Hpy8I GTNNAC 5 cut(s) 293, 374, 931, 1100, 1138
Hpy99I CGWCG 1 cut(s) 1093
HpyAV CCTTC 2 cut(s) 16, 259
HpyCH4III ACNGT 4 cut(s) 453, 641, 935, 1089
HpyF10VI GCNNNNNNNGC 7 cut(s) 299, 348, 482, 494, 497, 956, 1246
HpyF3I CTNAG 3 cut(s) 408, 472, 1056
Hsp92I GRCGYC 1 cut(s) 1281
Hsp92II CATG 8 cut(s) 246, 274, 418, 511, 524, 624, 1024, 1311
LguI GCTCTTC 1 cut(s) 50
LmnI GCTCC 2 cut(s) 246, 602
Lsp1109I GCAGC 4 cut(s) 475, 497, 509, 871
LweI GCATC 7 cut(s) 34, 175, 289, 598, 713, 735, 1312
MaeI CTAG 5 cut(s) 494, 561, 741, 960, 1001
MaeIII GTNAC 7 cut(s) 176, 191, 783, 935, 1007, 1083, 1151
MboII GAAGA 3 cut(s) 67, 495, 1229
MfeI CAATTG 1 cut(s) 391
MhlI GDGCHC 2 cut(s) 295, 599
MluCI AATT 4 cut(s) 391, 676, 757, 1062
MlyI GAGTC 1 cut(s) 961
MmeI TCCRAC 2 cut(s) 786, 1168
Mph1103I ATGCAT 1 cut(s) 49
MseI TTAA 2 cut(s) 516, 837
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 2 cut(s) 189, 592
MspR9I CCNGG 6 cut(s) 348, 459, 593, 822, 843, 986
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 1 cut(s) 342
MvaI CCWGG 5 cut(s) 348, 459, 822, 843, 986
MwoI GCNNNNNNNGC 7 cut(s) 299, 348, 482, 494, 497, 956, 1246
NciI CCSGG 1 cut(s) 593
NcoI CCATGG 3 cut(s) 507, 620, 1307
NdeI CATATG 2 cut(s) 773, 1213
NlaIII CATG 8 cut(s) 246, 274, 418, 511, 524, 624, 1024, 1311
NlaIV GGNNCC 1 cut(s) 598
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 49
NspI RCATGY 2 cut(s) 524, 1024
PaeR7I CTCGAG 1 cut(s) 1224
PceI AGGCCT 1 cut(s) 406
PciI ACATGT 1 cut(s) 1020
PciSI GCTCTTC 1 cut(s) 50
PctI GAATGC 1 cut(s) 342
PfeI GAWTC 5 cut(s) 128, 688, 791, 1208, 1220
PflMI CCANNNNNTGG 1 cut(s) 309
PkrI GCNGC 4 cut(s) 487, 490, 499, 861
PleI GAGTC 1 cut(s) 960
PpsI GAGTC 1 cut(s) 960
PscI ACATGT 1 cut(s) 1020
Psp6I CCWGG 5 cut(s) 346, 457, 820, 841, 984
PspGI CCWGG 5 cut(s) 346, 457, 820, 841, 984
PspN4I GGNNCC 1 cut(s) 598
PspPI GGNCC 4 cut(s) 76, 617, 1193, 1311
PstI CTGCAG 1 cut(s) 861
PstNI CAGNNNCTG 1 cut(s) 464
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 1 cut(s) 713
RsaNI GTAC 1 cut(s) 712
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 516, 837
SatI GCNGC 4 cut(s) 486, 489, 498, 860
Sau96I GGNCC 4 cut(s) 76, 617, 1193, 1311
SchI GAGTC 1 cut(s) 961
ScrFI CCNGG 6 cut(s) 348, 459, 593, 822, 843, 986
SduI GDGCHC 2 cut(s) 295, 599
SfaNI GCATC 7 cut(s) 34, 175, 289, 598, 713, 735, 1312
SfcI CTRYAG 1 cut(s) 857
Sfr274I CTCGAG 1 cut(s) 1224
SinI GGWCC 3 cut(s) 617, 1193, 1311
SlaI CTCGAG 1 cut(s) 1224
SmlI CTYRAG 1 cut(s) 1224
SmoI CTYRAG 1 cut(s) 1224
Sse9I AATT 4 cut(s) 391, 676, 757, 1062
SseBI AGGCCT 1 cut(s) 406
SspMI CTAG 5 cut(s) 494, 561, 741, 960, 1001
StuI AGGCCT 1 cut(s) 406
StyD4I CCNGG 6 cut(s) 346, 457, 591, 820, 841, 984
StyI CCWWGG 3 cut(s) 507, 620, 1307
TaaI ACNGT 4 cut(s) 453, 641, 935, 1089
TaqI TCGA 4 cut(s) 227, 307, 729, 1225
TasI AATT 4 cut(s) 391, 676, 757, 1062
TatI WGTACW 1 cut(s) 711
TfiI GAWTC 5 cut(s) 128, 688, 791, 1208, 1220
Tru1I TTAA 2 cut(s) 516, 837
Tru9I TTAA 2 cut(s) 516, 837
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 4 cut(s) 485, 488, 497, 859
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 5 cut(s) 739, 760, 1055, 1200, 1230
TspGWI ACGGA 2 cut(s) 332, 1143
Van91I CCANNNNNTGG 1 cut(s) 309
VneI GTGCAC 1 cut(s) 291
VpaK11BI GGWCC 3 cut(s) 617, 1193, 1311
XapI RAATTY 2 cut(s) 676, 1062
XceI RCATGY 2 cut(s) 524, 1024
XhoI CTCGAG 1 cut(s) 1224
XspI CTAG 5 cut(s) 494, 561, 741, 960, 1001
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.