Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000663

Physical Location & Seq

Genomic Coordinates Reverse (-)

1529651 .. 1533975

Gene Structure 4325 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1021500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGCGCGAAACCTCCTCTGCCTCCCGGGGAATGTTTCCGCATTTCGCGCCCCTCTAGCAGCCCTCTTCCTCCTTTCTCTTCTTTTCCACTCAACCAGCGCCGCCACCCACGACTACCACGACGCTCTCCGCAAGAGCATCCTCTTCTTCGAAGGCCAACGCTCCGGCAAGCTCCCCCCGGATCAACGCCTAAAATGGCGCCGCGACTCCGCATTGCACGACGGGTCCGCCGCCGGAGTGGACTTATCGGGCGGCTACTACGACGCCGGCGACAACGTAAAGTTCGGATTTCCAATGGCATTCACGACGACGTTGCTGGCGTGGAGCGTCATCGACTTCGGGAGAACCATGGGTCCGGAGCTGAAGAACGCGGTTCGGGCCGTGAAATGGGGCTCCGACTACCTCCTCAAGGCGACGGCGGTGCCCGACGTCGTTTTTGTCCAGGTCGGCGACCCGTACTCGGACCACAACTGCTGGGAGCGGCCGGAGGACATGGACACGTCACGCGCTGTGTACAAGATCGATAAGAACCACCCGGGGTCTGACGTGGCAGGCGAAACAGCCGCTGCTCTCGCCGCTGCTTCTATCGTGTTCAGATCACGTGACCCGGCTTACTCTAGATTCCTCCTCAATCGAGCCGTTAGGGTGTTCGAGTTTGCTGACAGGCACCGGGGTGCGTACAGCGCGAGCCTGCACCATGCCGTGTGCCCTTTTTACTGCGACGTGAACGGCTTCCAGGATGAGTTGCTGTGGGGAGCAGTGTGGCTGCACAAGGCATCGAGGAGGCGCGTATACAGAGAATATATAGTGAAGAACGAGGTGGTGTTGCGAGCAGGTGATACGATTAATGAGTTTGGTTGGGACAACAAGCATGCTGGGATTAACGTCCTCATTTCCAAGGAAGTGCTTATGGGAAAAGCAGATTATTTCGAATCTTTCACGCAGAATGCTGATGAGTTTATATGTTCCATACTGCCTGGACTTTCTCATCCTCAAGTCCAATATTCTCCTGGTGGCTTGATATTCAAGCCAGGAGGTAGTAACATGCAGCATGTGACCTCACTTTCATTCCTACTTCTGTCCTACTCCAACTATCTAAGCCATGCCAATAAGAACGTGCCATGTGGCGAGACCTCTGCCTCCCCTGCTTTCCTCAAACAATTGGCCAAACGCCAGGTGGACTACATTCTAGGTGATAATCCATTGAGGATGTCGTATATGGTGGGATACGGTGCACGCTACCCACAGAGGATACACCACCGGGGCAGCTCGCTACCATCGGTGCAGGCCCACCCGGCCCGTATCGGGTGTAAAGCCGGGTCTAGATACTTCTTGAGTCCAAATCCCAACCCAAATTTGCTGGTGGGAGCAGTGGTTGGTGGGCCCAACAGCTCAGACGCGTTCCCGGACTCGAGGCCTTTCTTCCAAGAATCCGAGCCCACCACTTACATCAATGCGCCGCTGGTGGGCCTACTTTCATATTTTTCAGCCCACTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008810 GO:0009507 GO:0009536 GO:0009825 GO:0009987 GO:0016043 GO:0016049 GO:0016787 GO:0016798 GO:0040007 GO:0042545 GO:0042547 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0071554 GO:0071555 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_hydro_9

Protein Analysis

500

Amino Acids

55.19

Weight (kDa)

8.66

Isoelectric Point (pI)

36.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_9	PF00759	39 - 492	7.5e-155	Glycosyl hydrolase family 9

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000663)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23210 AT1G70710
fragaria_vesca	FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica	MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica	Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis	pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis	RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata	RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora	Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii	Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa	Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha	Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana	Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	824
AatII	GACGTC	1 cut(s)	432
Acc36I	ACCTGC	1 cut(s)	824
AccB1I	GGYRCC	3 cut(s)	198, 421, 666
AccBSI	CCGCTC	1 cut(s)	481
AccI	GTMKAC	1 cut(s)	792
AccII	CGCG	8 cut(s)	7, 48, 204, 371, 507, 686, 789, 1400
AccIII	TCCGGA	1 cut(s)	355
AclWI	GGATC	1 cut(s)	189
AcoI	YGGCCR	2 cut(s)	482, 1164
AcsI	RAATTY	1 cut(s)	1354
AcuI	CTGAAG	1 cut(s)	383
AcvI	CACGTG	1 cut(s)	602
AcyI	GRCGYC	3 cut(s)	199, 264, 429
AfaI	GTAC	3 cut(s)	458, 515, 680
AfiI	CCNNNNNNNGG	5 cut(s)	387, 409, 460, 1305, 1466
AflIII	ACRYGT	2 cut(s)	498, 1398
AgsI	TTSAA	1 cut(s)	1027
AjiI	CACGTC	3 cut(s)	501, 547, 724
AjnI	CCWGG	6 cut(s)	441, 735, 976, 1009, 1030, 1173
AleI	CACNNNNGTG	1 cut(s)	672
AloI	GAACNNNNNNTCC	2 cut(s)	337, 369
AluBI	AGCT	4 cut(s)	172, 361, 1269, 1392
AluI	AGCT	4 cut(s)	172, 361, 1269, 1392
Alw21I	GWGCWC	1 cut(s)	1237
Alw26I	GTCTC	1 cut(s)	1124
Alw44I	GTGCAC	1 cut(s)	1233
AlwI	GGATC	1 cut(s)	189
AlwNI	CAGNNNCTG	1 cut(s)	566
Ama87I	CYCGRG	3 cut(s)	25, 535, 1411
Aor13HI	TCCGGA	1 cut(s)	355
AoxI	GGCC	9 cut(s)	154, 378, 482, 1164, 1287, 1296, 1382, 1415, 1468
ApaI	GGGCCC	1 cut(s)	1386
ApaLI	GTGCAC	1 cut(s)	1233
ApeKI	GCWGC	6 cut(s)	59, 566, 578, 766, 1048, 1266
ApoI	RAATTY	1 cut(s)	1354
ArsI	GACNNNNNNTTYG	2 cut(s)	419, 451
AseI	ATTAAT	1 cut(s)	846
Asp700I	GAANNNNTTC	1 cut(s)	731
AspLEI	GCGC	8 cut(s)	7, 50, 101, 201, 509, 686, 789, 1459
AspS9I	GGNCC	9 cut(s)	225, 353, 378, 463, 1288, 1297, 1382, 1383, 1468
AsuHPI	GGTGA	2 cut(s)	848, 1205
AsuII	TTCGAA	2 cut(s)	150, 930
AvaI	CYCGRG	3 cut(s)	25, 535, 1411
AvaII	GGWCC	3 cut(s)	225, 353, 463
BaeGI	GKGCMC	4 cut(s)	426, 710, 1237, 1386
BalI	TGGCCA	1 cut(s)	1166
BanI	GGYRCC	3 cut(s)	198, 421, 666
BanII	GRGCYC	3 cut(s)	395, 1386, 1440
BbrPI	CACGTG	1 cut(s)	602
Bbv12I	GWGCWC	1 cut(s)	1237
BbvI	GCAGC	6 cut(s)	71, 553, 565, 753, 1060, 1278
BccI	CCATC	1 cut(s)	1285
BceAI	ACGGC	5 cut(s)	365, 432, 623, 686, 745
BcgI	CGANNNNNNTGC	6 cut(s)	295, 329, 403, 437, 1118, 1152
BciT130I	CCWGG	6 cut(s)	443, 737, 978, 1011, 1032, 1175
BciVI	GTATCC	2 cut(s)	1220, 1245
BcoDI	GTCTC	1 cut(s)	1124
BfaI	CTAG	4 cut(s)	56, 618, 1190, 1323
BfoI	RGCGCY	2 cut(s)	102, 202
BfuAI	ACCTGC	1 cut(s)	824
BfuI	GTATCC	2 cut(s)	1220, 1245
BglI	GCCNNNNNGGC	1 cut(s)	1295
Bme18I	GGWCC	3 cut(s)	225, 353, 463
BmeT110I	CYCGRG	3 cut(s)	25, 535, 1411
BmgBI	CACGTC	3 cut(s)	501, 547, 724
BmgT120I	GGNCC	9 cut(s)	225, 353, 378, 463, 1288, 1297, 1382, 1383, 1468
BmiI	GGNNCC	7 cut(s)	200, 226, 354, 394, 423, 668, 1384
BmsI	GCATC	2 cut(s)	147, 785
Bpu14I	TTCGAA	2 cut(s)	150, 930
BpuEI	CTTGAG	3 cut(s)	392, 978, 1354
Bsa29I	ATCGAT	1 cut(s)	522
BsaAI	YACGTR	1 cut(s)	602
BsaHI	GRCGYC	3 cut(s)	199, 264, 429
BsaI	GGTCTC	1 cut(s)	1124
BsaJI	CCNNGG	9 cut(s)	25, 26, 177, 348, 535, 536, 670, 897, 1261
BsaWI	WCCGGW	1 cut(s)	355
BsaXI	ACNNNNNCTCC	2 cut(s)	1359, 1389
Bsc4I	CCNNNNNNNGG	5 cut(s)	387, 409, 460, 1305, 1466
Bse118I	RCCGGY	1 cut(s)	266
Bse3DI	GCAATG	1 cut(s)	212
BseAI	TCCGGA	1 cut(s)	355
BseBI	CCWGG	6 cut(s)	443, 737, 978, 1011, 1032, 1175
BseCI	ATCGAT	1 cut(s)	522
BseDI	CCNNGG	9 cut(s)	25, 26, 177, 348, 535, 536, 670, 897, 1261
BseGI	GGATG	4 cut(s)	138, 745, 988, 1215
BseLI	CCNNNNNNNGG	5 cut(s)	387, 409, 460, 1305, 1466
BseMI	GCAATG	1 cut(s)	212
BseMII	CTCAG	1 cut(s)	1407
BseRI	GAGGAG	4 cut(s)	5, 395, 617, 796
BseSI	GKGCMC	4 cut(s)	426, 710, 1237, 1386
BseX3I	CGGCCG	1 cut(s)	482
BseXI	GCAGC	6 cut(s)	71, 553, 565, 753, 1060, 1278
BseYI	CCCAGC	2 cut(s)	474, 875
BsgI	GTGCAG	3 cut(s)	677, 752, 1304
Bsh1236I	CGCG	8 cut(s)	7, 48, 204, 371, 507, 686, 789, 1400
Bsh1285I	CGRYCG	1 cut(s)	485
BshFI	GGCC	9 cut(s)	156, 380, 484, 1166, 1289, 1298, 1384, 1417, 1470
BshNI	GGYRCC	3 cut(s)	198, 421, 666
BshVI	ATCGAT	1 cut(s)	522
BsiEI	CGRYCG	1 cut(s)	485
BsiHKAI	GWGCWC	1 cut(s)	1237
BsiHKCI	CYCGRG	3 cut(s)	25, 535, 1411
BslFI	GGGAC	1 cut(s)	875
BslI	CCNNNNNNNGG	5 cut(s)	387, 409, 460, 1305, 1466
BsmAI	GTCTC	1 cut(s)	1124
BsmFI	GGGAC	1 cut(s)	875
BsmI	GAATGC	2 cut(s)	299, 952
BsnI	GGCC	9 cut(s)	156, 380, 484, 1166, 1289, 1298, 1384, 1417, 1470
Bso31I	GGTCTC	1 cut(s)	1124
BsoBI	CYCGRG	3 cut(s)	25, 535, 1411
Bsp119I	TTCGAA	2 cut(s)	150, 930
Bsp120I	GGGCCC	1 cut(s)	1382
Bsp1286I	GDGCHC	6 cut(s)	395, 426, 710, 1237, 1386, 1440
Bsp13I	TCCGGA	1 cut(s)	355
Bsp1407I	TGTACA	1 cut(s)	513
Bsp143I	GATC	3 cut(s)	181, 519, 596
Bsp19I	CCATGG	1 cut(s)	348
BspANI	GGCC	9 cut(s)	156, 380, 484, 1166, 1289, 1298, 1384, 1417, 1470
BspCNI	CTCAG	1 cut(s)	1406
BspDI	ATCGAT	1 cut(s)	522
BspEI	TCCGGA	1 cut(s)	355
BspFNI	CGCG	8 cut(s)	7, 48, 204, 371, 507, 686, 789, 1400
BspLI	GGNNCC	7 cut(s)	200, 226, 354, 394, 423, 668, 1384
BspMI	ACCTGC	1 cut(s)	824
BspPI	GGATC	1 cut(s)	189
BspT104I	TTCGAA	2 cut(s)	150, 930
BspT107I	GGYRCC	3 cut(s)	198, 421, 666
BspTNI	GGTCTC	1 cut(s)	1124
BsrBI	CCGCTC	1 cut(s)	481
BsrDI	GCAATG	1 cut(s)	212
BsrFI	RCCGGY	1 cut(s)	266
BsrGI	TGTACA	1 cut(s)	513
BssAI	RCCGGY	1 cut(s)	266
BssECI	CCNNGG	9 cut(s)	25, 26, 177, 348, 535, 536, 670, 897, 1261
BssMI	GATC	3 cut(s)	181, 519, 596
BssNAI	GTATAC	1 cut(s)	793
BssNI	GRCGYC	3 cut(s)	199, 264, 429
BssT1I	CCWWGG	2 cut(s)	348, 897
Bst1107I	GTATAC	1 cut(s)	793
Bst2UI	CCWGG	6 cut(s)	443, 737, 978, 1011, 1032, 1175
Bst4CI	ACNGT	1 cut(s)	1232
Bst6I	CTCTTC	3 cut(s)	71, 84, 149
BstACI	GRCGYC	3 cut(s)	199, 264, 429
BstAUI	TGTACA	1 cut(s)	513
BstBAI	YACGTR	1 cut(s)	602
BstBI	TTCGAA	2 cut(s)	150, 930
BstDEI	CTNAG	2 cut(s)	1097, 1393
BstDSI	CCRYGG	1 cut(s)	348
BstENI	CCTNNNNNAGG	1 cut(s)	407
BstF5I	GGATG	4 cut(s)	138, 745, 988, 1215
BstFNI	CGCG	8 cut(s)	7, 48, 204, 371, 507, 686, 789, 1400
BstH2I	RGCGCY	2 cut(s)	102, 202
BstHHI	GCGC	8 cut(s)	7, 50, 101, 201, 509, 686, 789, 1459
BstKTI	GATC	3 cut(s)	184, 522, 599
BstMAI	GTCTC	1 cut(s)	1124
BstMBI	GATC	3 cut(s)	181, 519, 596
BstMCI	CGRYCG	1 cut(s)	485
BstMWI	GCNNNNNNNGC	8 cut(s)	47, 56, 377, 572, 575, 683, 1145, 1295
BstNI	CCWGG	6 cut(s)	443, 737, 978, 1011, 1032, 1175
BstNSI	RCATGY	3 cut(s)	875, 1048, 1055
BstSLI	GKGCMC	4 cut(s)	426, 710, 1237, 1386
BstUI	CGCG	8 cut(s)	7, 48, 204, 371, 507, 686, 789, 1400
BstV1I	GCAGC	6 cut(s)	71, 553, 565, 753, 1060, 1278
BstZ17I	GTATAC	1 cut(s)	793
BstZI	CGGCCG	1 cut(s)	482
Bsu15I	ATCGAT	1 cut(s)	522
BsuI	GTATCC	2 cut(s)	1220, 1245
BsuRI	GGCC	9 cut(s)	156, 380, 484, 1166, 1289, 1298, 1384, 1417, 1470
BsuTUI	ATCGAT	1 cut(s)	522
BtgI	CCRYGG	1 cut(s)	348
BtrI	CACGTC	3 cut(s)	501, 547, 724
BtsCI	GGATG	4 cut(s)	138, 745, 988, 1215
BtsI	GCAGTG	2 cut(s)	765, 1377
BtsIMutI	CAGTG	2 cut(s)	765, 1377
BveI	ACCTGC	1 cut(s)	824
CaiI	CAGNNNCTG	1 cut(s)	566
CfoI	GCGC	8 cut(s)	7, 50, 101, 201, 509, 686, 789, 1459
Cfr10I	RCCGGY	1 cut(s)	266
Cfr13I	GGNCC	9 cut(s)	225, 353, 378, 463, 1288, 1297, 1382, 1383, 1468
Cfr9I	CCCGGG	2 cut(s)	25, 535
ClaI	ATCGAT	1 cut(s)	522
CseI	GACGC	4 cut(s)	131, 272, 316, 1406
Csp6I	GTAC	3 cut(s)	457, 514, 679
CviAII	CATG	8 cut(s)	349, 493, 698, 872, 1045, 1052, 1103, 1122
CviQI	GTAC	3 cut(s)	457, 514, 679
DdeI	CTNAG	2 cut(s)	1097, 1393
DinI	GGCGCC	1 cut(s)	200
DpnI	GATC	3 cut(s)	183, 521, 598
DpnII	GATC	3 cut(s)	181, 519, 596
EaeI	YGGCCR	2 cut(s)	482, 1164
EagI	CGGCCG	1 cut(s)	482
Eam1104I	CTCTTC	3 cut(s)	71, 84, 149
EarI	CTCTTC	3 cut(s)	71, 84, 149
EciI	GGCGGA	1 cut(s)	217
EclXI	CGGCCG	1 cut(s)	482
Eco130I	CCWWGG	2 cut(s)	348, 897
Eco147I	AGGCCT	1 cut(s)	1417
Eco24I	GRGCYC	3 cut(s)	395, 1386, 1440
Eco31I	GGTCTC	1 cut(s)	1124
Eco47I	GGWCC	3 cut(s)	225, 353, 463
Eco52I	CGGCCG	1 cut(s)	482
Eco57I	CTGAAG	1 cut(s)	383
Eco72I	CACGTG	1 cut(s)	602
Eco88I	CYCGRG	3 cut(s)	25, 535, 1411
EcoNI	CCTNNNNNAGG	1 cut(s)	407
EcoRII	CCWGG	6 cut(s)	441, 735, 976, 1009, 1030, 1173
EcoT14I	CCWWGG	2 cut(s)	348, 897
EcoT38I	GRGCYC	3 cut(s)	395, 1386, 1440
EgeI	GGCGCC	1 cut(s)	200
EheI	GGCGCC	1 cut(s)	200
ErhI	CCWWGG	2 cut(s)	348, 897
FaeI	CATG	8 cut(s)	352, 496, 701, 875, 1048, 1055, 1106, 1125
FaqI	GGGAC	1 cut(s)	875
FatI	CATG	8 cut(s)	348, 492, 697, 871, 1044, 1051, 1102, 1121
FblI	GTMKAC	1 cut(s)	792
FokI	GGATG	4 cut(s)	125, 752, 975, 1222
FriOI	GRGCYC	3 cut(s)	395, 1386, 1440
FspBI	CTAG	4 cut(s)	56, 618, 1190, 1323
GlaI	GCGC	8 cut(s)	6, 49, 100, 200, 508, 685, 788, 1458
GsaI	CCCAGC	2 cut(s)	478, 879
HaeII	RGCGCY	2 cut(s)	102, 202
HaeIII	GGCC	9 cut(s)	156, 380, 484, 1166, 1289, 1298, 1384, 1417, 1470
HgaI	GACGC	4 cut(s)	131, 272, 316, 1406
HhaI	GCGC	8 cut(s)	7, 50, 101, 201, 509, 686, 789, 1459
Hin1I	GRCGYC	3 cut(s)	199, 264, 429
Hin1II	CATG	8 cut(s)	352, 496, 701, 875, 1048, 1055, 1106, 1125
Hin6I	GCGC	8 cut(s)	5, 48, 99, 199, 507, 684, 787, 1457
HinP1I	GCGC	8 cut(s)	5, 48, 99, 199, 507, 684, 787, 1457
HinfI	GANTC	6 cut(s)	206, 621, 932, 1336, 1409, 1430
HphI	GGTGA	2 cut(s)	848, 1205
Hpy166II	GTNNAC	6 cut(s)	241, 514, 727, 793, 1180, 1235
Hpy188I	TCNGA	7 cut(s)	287, 397, 463, 544, 596, 1396, 1435
Hpy188III	TCNNGA	6 cut(s)	304, 340, 356, 618, 1323, 1333
Hpy8I	GTNNAC	6 cut(s)	241, 514, 727, 793, 1180, 1235
Hpy99I	CGWCG	9 cut(s)	125, 224, 266, 310, 313, 418, 431, 434, 725
HpyAV	CCTTC	1 cut(s)	146
HpyCH4III	ACNGT	1 cut(s)	1232
HpyCH4IV	ACGT	9 cut(s)	276, 311, 429, 500, 546, 601, 723, 885, 1116
HpyCH4V	TGCA	6 cut(s)	217, 694, 769, 1048, 1235, 1285
HpyF10VI	GCNNNNNNNGC	8 cut(s)	47, 56, 377, 572, 575, 683, 1145, 1295
HpyF3I	CTNAG	2 cut(s)	1097, 1393
HpySE526I	ACGT	9 cut(s)	276, 311, 429, 500, 546, 601, 723, 885, 1116
Hsp92I	GRCGYC	3 cut(s)	199, 264, 429
Hsp92II	CATG	8 cut(s)	352, 496, 701, 875, 1048, 1055, 1106, 1125
HspAI	GCGC	8 cut(s)	5, 48, 99, 199, 507, 684, 787, 1457
KasI	GGCGCC	1 cut(s)	198
Kpn2I	TCCGGA	1 cut(s)	355
KroI	GCCGGC	1 cut(s)	266
KroNI	GCCGGC	1 cut(s)	268
Kzo9I	GATC	3 cut(s)	181, 519, 596
LmnI	GCTCC	8 cut(s)	167, 177, 324, 358, 398, 478, 755, 1367
Lsp1109I	GCAGC	6 cut(s)	71, 553, 565, 753, 1060, 1278
LweI	GCATC	2 cut(s)	147, 785
MaeI	CTAG	4 cut(s)	56, 618, 1190, 1323
MaeII	ACGT	9 cut(s)	276, 311, 429, 500, 546, 601, 723, 885, 1116
MaeIII	GTNAC	4 cut(s)	501, 602, 1040, 1054
MalI	GATC	3 cut(s)	183, 521, 598
MbiI	CCGCTC	1 cut(s)	481
MboI	GATC	3 cut(s)	181, 519, 596
MboII	GAAGA	7 cut(s)	58, 71, 136, 139, 376, 823, 1414
MfeI	CAATTG	1 cut(s)	1160
MhlI	GDGCHC	6 cut(s)	395, 426, 710, 1237, 1386, 1440
MlsI	TGGCCA	1 cut(s)	1166
MluCI	AATT	2 cut(s)	1160, 1354
MluI	ACGCGT	1 cut(s)	1398
MluNI	TGGCCA	1 cut(s)	1166
Mly113I	GGCGCC	1 cut(s)	199
MlyI	GAGTC	3 cut(s)	200, 1345, 1403
MmeI	TCCRAC	2 cut(s)	420, 1113
Mox20I	TGGCCA	1 cut(s)	1166
MreI	CGCCGGCG	1 cut(s)	266
MroI	TCCGGA	1 cut(s)	355
MroNI	GCCGGC	1 cut(s)	266
MroXI	GAANNNNTTC	1 cut(s)	731
MscI	TGGCCA	1 cut(s)	1166
MseI	TTAA	2 cut(s)	846, 882
MslI	CAYNNNNRTG	1 cut(s)	672
Msp20I	TGGCCA	1 cut(s)	1166
MspA1I	CMGCKG	3 cut(s)	566, 578, 1462
MunI	CAATTG	1 cut(s)	1160
Mva1269I	GAATGC	2 cut(s)	299, 952
MvaI	CCWGG	6 cut(s)	443, 737, 978, 1011, 1032, 1175
MvnI	CGCG	8 cut(s)	7, 48, 204, 371, 507, 686, 789, 1400
MwoI	GCNNNNNNNGC	8 cut(s)	47, 56, 377, 572, 575, 683, 1145, 1295
NaeI	GCCGGC	1 cut(s)	268
NarI	GGCGCC	1 cut(s)	199
NcoI	CCATGG	1 cut(s)	348
NdeII	GATC	3 cut(s)	181, 519, 596
NgoMIV	GCCGGC	1 cut(s)	266
NlaIII	CATG	8 cut(s)	352, 496, 701, 875, 1048, 1055, 1106, 1125
NlaIV	GGNNCC	7 cut(s)	200, 226, 354, 394, 423, 668, 1384
NmuCI	GTSAC	3 cut(s)	501, 602, 1054
NspI	RCATGY	3 cut(s)	875, 1048, 1055
NspV	TTCGAA	2 cut(s)	150, 930
OliI	CACNNNNGTG	1 cut(s)	672
PaeI	GCATGC	1 cut(s)	875
PaeR7I	CTCGAG	1 cut(s)	1411
PaqCI	CACCTGC	1 cut(s)	824
PceI	AGGCCT	1 cut(s)	1417
PcsI	WCGNNNNNNNCGW	4 cut(s)	117, 267, 317, 552
PctI	GAATGC	2 cut(s)	299, 952
PdiI	GCCGGC	1 cut(s)	268
PdmI	GAANNNNTTC	1 cut(s)	731
PfeI	GAWTC	3 cut(s)	621, 932, 1430
PfoI	TCCNGGA	2 cut(s)	735, 1404
PleI	GAGTC	3 cut(s)	200, 1344, 1403
PluTI	GGCGCC	1 cut(s)	202
PmaCI	CACGTG	1 cut(s)	602
PmlI	CACGTG	1 cut(s)	602
PpsI	GAGTC	3 cut(s)	200, 1344, 1403
Ppu21I	YACGTR	1 cut(s)	602
PshBI	ATTAAT	1 cut(s)	846
Psp6I	CCWGG	6 cut(s)	441, 735, 976, 1009, 1030, 1173
PspCI	CACGTG	1 cut(s)	602
PspFI	CCCAGC	2 cut(s)	474, 875
PspGI	CCWGG	6 cut(s)	441, 735, 976, 1009, 1030, 1173
PspN4I	GGNNCC	7 cut(s)	200, 226, 354, 394, 423, 668, 1384
PspOMI	GGGCCC	1 cut(s)	1382
PspPI	GGNCC	9 cut(s)	225, 353, 378, 463, 1288, 1297, 1382, 1383, 1468
PspXI	VCTCGAGB	1 cut(s)	1411
PstNI	CAGNNNCTG	1 cut(s)	566
RsaI	GTAC	3 cut(s)	458, 515, 680
RsaNI	GTAC	3 cut(s)	457, 514, 679
RseI	CAYNNNNRTG	1 cut(s)	672
SaqAI	TTAA	2 cut(s)	846, 882
Sau3AI	GATC	3 cut(s)	181, 519, 596
Sau96I	GGNCC	9 cut(s)	225, 353, 378, 463, 1288, 1297, 1382, 1383, 1468
SchI	GAGTC	3 cut(s)	200, 1345, 1403
SduI	GDGCHC	6 cut(s)	395, 426, 710, 1237, 1386, 1440
SfaNI	GCATC	2 cut(s)	147, 785
SfiI	GGCCNNNNNGGCC	1 cut(s)	1295
SfoI	GGCGCC	1 cut(s)	200
Sfr274I	CTCGAG	1 cut(s)	1411
SfuI	TTCGAA	2 cut(s)	150, 930
SgrAI	CRCCGGYG	1 cut(s)	266
SinI	GGWCC	3 cut(s)	225, 353, 463
SlaI	CTCGAG	1 cut(s)	1411
SmaI	CCCGGG	2 cut(s)	27, 537
SmiMI	CAYNNNNRTG	1 cut(s)	672
SmlI	CTYRAG	4 cut(s)	407, 993, 1333, 1411
SmoI	CTYRAG	4 cut(s)	407, 993, 1333, 1411
SphI	GCATGC	1 cut(s)	875
Sse9I	AATT	2 cut(s)	1160, 1354
SseBI	AGGCCT	1 cut(s)	1417
SspDI	GGCGCC	1 cut(s)	198
SspI	AATATT	1 cut(s)	1004
SspMI	CTAG	4 cut(s)	56, 618, 1190, 1323
StuI	AGGCCT	1 cut(s)	1417
StyI	CCWWGG	2 cut(s)	348, 897
TaaI	ACNGT	1 cut(s)	1232
TaiI	ACGT	9 cut(s)	279, 314, 432, 503, 549, 604, 726, 888, 1119
TaqI	TCGA	8 cut(s)	150, 333, 522, 634, 651, 779, 930, 1412
TasI	AATT	2 cut(s)	1160, 1354
TatI	WGTACW	1 cut(s)	513
TauI	GCSGC	8 cut(s)	104, 204, 233, 255, 484, 566, 578, 1462
TfiI	GAWTC	3 cut(s)	621, 932, 1430
Tru1I	TTAA	2 cut(s)	846, 882
Tru9I	TTAA	2 cut(s)	846, 882
TscAI	CASTG	2 cut(s)	765, 1377
TseFI	GTSAC	3 cut(s)	501, 602, 1054
TseI	GCWGC	6 cut(s)	59, 566, 578, 766, 1048, 1266
Tsp45I	GTSAC	3 cut(s)	501, 602, 1054
TspDTI	ATGAA	2 cut(s)	1056, 1467
TspMI	CCCGGG	2 cut(s)	25, 535
TspRI	CASTG	2 cut(s)	765, 1377
VneI	GTGCAC	1 cut(s)	1233
VpaK11BI	GGWCC	3 cut(s)	225, 353, 463
VspI	ATTAAT	1 cut(s)	846
XagI	CCTNNNNNAGG	1 cut(s)	407
XapI	RAATTY	1 cut(s)	1354
XbaI	TCTAGA	2 cut(s)	617, 1322
XceI	RCATGY	3 cut(s)	875, 1048, 1055
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1007, 1174
XhoI	CTCGAG	1 cut(s)	1411
XmaI	CCCGGG	2 cut(s)	25, 535
XmiI	GTMKAC	1 cut(s)	792
XmnI	GAANNNNTTC	1 cut(s)	731
XspI	CTAG	4 cut(s)	56, 618, 1190, 1323
ZraI	GACGTC	1 cut(s)	430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.