Rroxscaffold_4G00283360

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000663
Physical Location & Seq
Reverse (-)
5287134 .. 5289530
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283360.1

Sequence Viewer

Length: 1491 bp
ATGTGTACCAATTTCCTACTGTTAAGGCTGTTGATAGCAGCAGGGTTGTTGGCAATGGCAACCGCCGCAAAAGTGACTCCACATAACTACGCAGATGCTTTGTCGAAGTCCATTTTGTTTTTTGAAGGCCAGAGATCTGGCAAGTTACCGAATTCTCAACGAATGAATTGGAGGAAAGATTCTGCCCTACATGATGGCAGCGATGTTCATAGGGATCTGGTGGGAGGTTACTATGATGCTGGTGACAATGTGAAGTTCAACTTTCCCATGGCCTTCACAACAACATTGTTGTCATGGAGCGTTCTAGAGTTTGGAGATTTGATGGGATCTGAACTTCAACATGCTTTGGAAGCTATAAGATGGGCAACTGATTATTTTCTTAAAGCCACAAATGATCCTGGTTCGGTAGTTGGCGTAGTAGACATGGATACCCCAAGAACCACATATGTAGTAACTAAAGAAAAACCAGGTTCAGAAGTTTCTGCGGAGATCGCAGCCGCGCTTGCAGCTTCCTCCATAGTGTTTAACCAATCAGACAAAGCCTACTCTGACTCGCTTCTTAAGCGAGCCATCCAAGTGTTTCAATTTGCAGAGCAGTATCAGGGATCTTACAATACTAGTATTGGCGACGGAGCCTGCCCATTTTACTGCGATTTTAGTGGCTACATGGATGAATTGATTTGGGGAGCTTCATGGCTATACAAGGCAACAAAGGCACCAAATTATTGGAAATATGTCAAAGACCACATACACAATCTGGAGTCGTCAAACACTGTAGTGAGAAATTTGGATGGTTCTCGGCCTGTTCCATTTGTTGGTGGTGACATTACTGAATTCGGATGGGATAGCAAGCATGCCGGCATCAACATACTTGTTTCTCAATGGGTTATGACTGATCCAGCCAATTCCAATCCGTTCATTCTCAAAGCTGACCAGCTTATATGCTCCATTCTGCCTGAATCACCAGCTCCCAAATCAGTCACGTATTCACCAGGTGGACTCTTGTTCAAACCTGGATCTTGTAACCTGCAACACACGACATCCTTATCATTTCTTCTTATAGTTTATGCTCGATACCTGAAAGGTGCCAACAGAACCATAAACTGTGGCAATAATGTTGTTCTTGGTCCAGCTCATTTGATAGATTTCACAAGAAAACAGGTTGATTATATACTCGGAAGCAACCCATTGGGGATGTCTTATATGGTGGGATATGGTACAAAATTTCCTCAAAGAATACATCATCGTGCTTCTGTGGTACCTTCCATGGATAAGCATCCACAGCACATGAAGTGCCATGATGGCGATCAGTATTTCAAAAGCCAAGCTCCGAATCAAAACTTGCTAATAGGAGCAGTTGTCGGGGGACCAGCCGAGAATGATACATTCGAAGATTCTCGATATGATCCTGGACAATCAGAGCCAGTTACATACAACAATGCCCCTTTTGTTGGTGTTTTGGCATTCTTTAAGGCTCTATATAATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

496

Amino Acids

54.69

Weight (kDa)

6.27

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 487 7.2e-136 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1035
Acc65I GGTACC 1 cut(s) 1258
AccB1I GGYRCC 3 cut(s) 715, 1085, 1258
AccB7I CCANNNNNTGG 1 cut(s) 815
AccI GTMKAC 1 cut(s) 420
AccII CGCG 1 cut(s) 500
AciI CCGC 4 cut(s) 63, 66, 485, 498
AclWI GGATC 7 cut(s) 222, 334, 389, 613, 890, 1024, 1400
AcsI RAATTY 4 cut(s) 151, 784, 833, 1223
AdeI CACNNNGTG 1 cut(s) 995
AfaI GTAC 3 cut(s) 7, 1219, 1260
AfiI CCNNNNNNNGG 2 cut(s) 815, 1451
AflII CTTAAG 1 cut(s) 560
AgsI TTSAA 6 cut(s) 125, 259, 338, 584, 1009, 1318
AhlI ACTAGT 1 cut(s) 617
AjnI CCWGG 5 cut(s) 397, 466, 991, 1012, 1408
AleI CACNNNNGTG 1 cut(s) 776
AluBI AGCT 8 cut(s) 353, 509, 689, 929, 937, 968, 1133, 1328
AluI AGCT 8 cut(s) 353, 509, 689, 929, 937, 968, 1133, 1328
AlwI GGATC 7 cut(s) 222, 334, 389, 613, 890, 1024, 1400
AoxI GGCC 3 cut(s) 127, 270, 800
ApeKI GCWGC 4 cut(s) 38, 198, 494, 506
ApoI RAATTY 4 cut(s) 151, 784, 833, 1223
Asp718I GGTACC 1 cut(s) 1258
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 2 cut(s) 1127, 1367
AsuHPI GGTGA 4 cut(s) 254, 833, 954, 981
AsuII TTCGAA 1 cut(s) 1389
AvaII GGWCC 2 cut(s) 1127, 1367
BanI GGYRCC 3 cut(s) 715, 1085, 1258
BbvI GCAGC 4 cut(s) 50, 210, 506, 518
BccI CCATC 7 cut(s) 188, 316, 354, 578, 785, 834, 1295
BciT130I CCWGG 5 cut(s) 399, 468, 993, 1014, 1410
BciVI GTATCC 1 cut(s) 421
BcuI ACTAGT 1 cut(s) 617
BfaI CTAG 2 cut(s) 305, 618
BfmI CTRYAG 1 cut(s) 774
BfrI CTTAAG 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 1035
BfuI GTATCC 1 cut(s) 421
BglI GCCNNNNNGGC 1 cut(s) 1302
BglII AGATCT 1 cut(s) 134
BisI GCNGC 6 cut(s) 39, 66, 199, 495, 498, 507
BlsI GCNGC 6 cut(s) 40, 67, 200, 496, 499, 508
Bme1390I CCNGG 5 cut(s) 399, 468, 993, 1014, 1410
Bme18I GGWCC 2 cut(s) 1127, 1367
BmgT120I GGNCC 2 cut(s) 1127, 1367
BmiI GGNNCC 5 cut(s) 634, 717, 1087, 1260, 1368
BmrFI CCNGG 5 cut(s) 399, 468, 993, 1014, 1410
BmsI GCATC 4 cut(s) 85, 226, 870, 1285
BpmI CTGGAG 1 cut(s) 779
Bpu14I TTCGAA 1 cut(s) 1389
BsaAI YACGTR 1 cut(s) 984
BsaBI GATNNNNATC 2 cut(s) 1275, 1305
BsaJI CCNNGG 2 cut(s) 267, 1266
BsaXI ACNNNNNCTCC 2 cut(s) 1344, 1374
Bsc4I CCNNNNNNNGG 2 cut(s) 815, 1451
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 1 cut(s) 1424
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 2 cut(s) 1275, 1305
BseBI CCWGG 5 cut(s) 399, 468, 993, 1014, 1410
BseDI CCNNGG 2 cut(s) 267, 1266
BseGI GGATG 7 cut(s) 570, 676, 796, 845, 1040, 1200, 1276
BseJI GATNNNNATC 2 cut(s) 1275, 1305
BseLI CCNNNNNNNGG 2 cut(s) 815, 1451
BseMI GCAATG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 1424
BseXI GCAGC 4 cut(s) 50, 210, 506, 518
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 3 cut(s) 129, 272, 802
BshNI GGYRCC 3 cut(s) 715, 1085, 1258
BsiSI CCGG 1 cut(s) 858
BslFI GGGAC 1 cut(s) 1380
BslI CCNNNNNNNGG 2 cut(s) 815, 1451
BsmFI GGGAC 1 cut(s) 1380
BsmI GAATGC 1 cut(s) 1463
BsnI GGCC 3 cut(s) 129, 272, 802
Bsp119I TTCGAA 1 cut(s) 1389
Bsp19I CCATGG 2 cut(s) 267, 1266
BspACI CCGC 4 cut(s) 63, 66, 485, 498
BspANI GGCC 3 cut(s) 129, 272, 802
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 5 cut(s) 634, 717, 1087, 1260, 1368
BspMI ACCTGC 1 cut(s) 1035
BspPI GGATC 7 cut(s) 222, 334, 389, 613, 890, 1024, 1400
BspT104I TTCGAA 1 cut(s) 1389
BspT107I GGYRCC 3 cut(s) 715, 1085, 1258
BspTI CTTAAG 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 857
BsrI ACTGG 1 cut(s) 1424
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 2 cut(s) 267, 1266
BssT1I CCWWGG 2 cut(s) 267, 1266
Bst2UI CCWGG 5 cut(s) 399, 468, 993, 1014, 1410
Bst4CI ACNGT 3 cut(s) 21, 775, 1106
BstAFI CTTAAG 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 984
BstBI TTCGAA 1 cut(s) 1389
BstC8I GCNNGC 6 cut(s) 504, 567, 637, 851, 855, 859
BstDSI CCRYGG 2 cut(s) 267, 1266
BstF5I GGATG 7 cut(s) 570, 676, 796, 845, 1040, 1200, 1276
BstFNI CGCG 1 cut(s) 500
BstHHI GCGC 1 cut(s) 502
BstMWI GCNNNNNNNGC 9 cut(s) 65, 350, 491, 503, 506, 562, 713, 1282, 1302
BstNI CCWGG 5 cut(s) 399, 468, 993, 1014, 1410
BstNSI RCATGY 2 cut(s) 344, 857
BstSCI CCNGG 5 cut(s) 397, 466, 991, 1012, 1408
BstSFI CTRYAG 1 cut(s) 774
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 4 cut(s) 50, 210, 506, 518
BstX2I RGATCY 5 cut(s) 134, 214, 326, 605, 1016
BstXI CCANNNNNNTGG 2 cut(s) 137, 726
BstYI RGATCY 5 cut(s) 134, 214, 326, 605, 1016
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 3 cut(s) 129, 272, 802
BtgI CCRYGG 2 cut(s) 267, 1266
BtgZI GCGATG 1 cut(s) 216
BtsCI GGATG 7 cut(s) 570, 676, 796, 845, 1040, 1200, 1276
BtsIMutI CAGTG 1 cut(s) 771
BveI ACCTGC 1 cut(s) 1035
Cac8I GCNNGC 6 cut(s) 504, 567, 637, 851, 855, 859
CfoI GCGC 1 cut(s) 502
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 2 cut(s) 1127, 1367
CsiI ACCWGGT 2 cut(s) 466, 991
Csp6I GTAC 3 cut(s) 6, 1218, 1259
CviQI GTAC 3 cut(s) 6, 1218, 1259
DraIII CACNNNGTG 1 cut(s) 995
Eco130I CCWWGG 2 cut(s) 267, 1266
Eco47I GGWCC 2 cut(s) 1127, 1367
EcoRI GAATTC 2 cut(s) 151, 833
EcoRII CCWGG 5 cut(s) 397, 466, 991, 1012, 1408
EcoT14I CCWWGG 2 cut(s) 267, 1266
ErhI CCWWGG 2 cut(s) 267, 1266
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 1380
FauNDI CATATG 1 cut(s) 445
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 6 cut(s) 39, 66, 199, 495, 498, 507
FokI GGATG 7 cut(s) 557, 683, 803, 852, 1027, 1207, 1263
Fsp4HI GCNGC 6 cut(s) 39, 66, 199, 495, 498, 507
FspBI CTAG 2 cut(s) 305, 618
GlaI GCGC 1 cut(s) 501
GluI GCNGC 6 cut(s) 39, 66, 199, 495, 498, 507
GsuI CTGGAG 1 cut(s) 779
HaeIII GGCC 3 cut(s) 129, 272, 802
HapII CCGG 1 cut(s) 858
HhaI GCGC 1 cut(s) 502
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HinfI GANTC 8 cut(s) 76, 179, 551, 761, 959, 999, 1333, 1394
HpaII CCGG 1 cut(s) 858
HphI GGTGA 4 cut(s) 254, 833, 954, 981
Hpy166II GTNNAC 3 cut(s) 6, 421, 998
Hpy188I TCNGA 8 cut(s) 331, 475, 535, 550, 839, 1178, 1332, 1420
Hpy188III TCNNGA 3 cut(s) 305, 758, 1398
Hpy8I GTNNAC 3 cut(s) 6, 421, 998
Hpy99I CGWCG 1 cut(s) 632
HpyAV CCTTC 3 cut(s) 119, 283, 1272
HpyCH4III ACNGT 3 cut(s) 21, 775, 1106
HpyCH4IV ACGT 1 cut(s) 983
HpyCH4V TGCA 3 cut(s) 506, 590, 1030
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 350, 491, 503, 506, 562, 713, 1282, 1302
HpySE526I ACGT 1 cut(s) 983
HspAI GCGC 1 cut(s) 500
KpnI GGTACC 1 cut(s) 1262
KroI GCCGGC 1 cut(s) 857
KroNI GCCGGC 1 cut(s) 859
LmnI GCTCC 7 cut(s) 297, 632, 686, 950, 973, 1333, 1352
Lsp1109I GCAGC 4 cut(s) 50, 210, 506, 518
LweI GCATC 4 cut(s) 85, 226, 870, 1285
MabI ACCWGGT 2 cut(s) 466, 991
MaeI CTAG 2 cut(s) 305, 618
MaeII ACGT 1 cut(s) 983
MaeIII GTNAC 9 cut(s) 73, 144, 227, 242, 451, 821, 979, 1022, 1426
MboII GAAGA 2 cut(s) 1046, 1403
MflI RGATCY 5 cut(s) 134, 214, 326, 605, 1016
MlyI GAGTC 4 cut(s) 70, 545, 770, 993
MnlI CCTC 4 cut(s) 165, 218, 523, 1239
MroNI GCCGGC 1 cut(s) 857
MseI TTAA 5 cut(s) 23, 381, 525, 561, 1470
MslI CAYNNNNRTG 3 cut(s) 575, 776, 1245
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 1 cut(s) 858
MspR9I CCNGG 5 cut(s) 399, 468, 993, 1014, 1410
Mva1269I GAATGC 1 cut(s) 1463
MvaI CCWGG 5 cut(s) 399, 468, 993, 1014, 1410
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 9 cut(s) 65, 350, 491, 503, 506, 562, 713, 1282, 1302
NaeI GCCGGC 1 cut(s) 859
NcoI CCATGG 2 cut(s) 267, 1266
NdeI CATATG 1 cut(s) 445
NgoMIV GCCGGC 1 cut(s) 857
NlaIV GGNNCC 5 cut(s) 634, 717, 1087, 1260, 1368
NmeAIII GCCGAG 2 cut(s) 778, 1399
NmuCI GTSAC 4 cut(s) 73, 242, 821, 979
NspI RCATGY 2 cut(s) 344, 857
NspV TTCGAA 1 cut(s) 1389
OliI CACNNNNGTG 1 cut(s) 776
PaeI GCATGC 1 cut(s) 857
PctI GAATGC 1 cut(s) 1463
PdiI GCCGGC 1 cut(s) 859
PfeI GAWTC 4 cut(s) 179, 959, 1333, 1394
PflMI CCANNNNNTGG 1 cut(s) 815
PfoI TCCNGGA 1 cut(s) 1408
PkrI GCNGC 6 cut(s) 40, 67, 200, 496, 499, 508
PleI GAGTC 4 cut(s) 70, 545, 769, 993
PpsI GAGTC 4 cut(s) 70, 545, 769, 993
Ppu21I YACGTR 1 cut(s) 984
Psp6I CCWGG 5 cut(s) 397, 466, 991, 1012, 1408
PspGI CCWGG 5 cut(s) 397, 466, 991, 1012, 1408
PspN4I GGNNCC 5 cut(s) 634, 717, 1087, 1260, 1368
PspPI GGNCC 2 cut(s) 1127, 1367
PsuI RGATCY 5 cut(s) 134, 214, 326, 605, 1016
RsaI GTAC 3 cut(s) 7, 1219, 1260
RsaNI GTAC 3 cut(s) 6, 1218, 1259
RseI CAYNNNNRTG 3 cut(s) 575, 776, 1245
SaqAI TTAA 5 cut(s) 23, 381, 525, 561, 1470
SatI GCNGC 6 cut(s) 39, 66, 199, 495, 498, 507
Sau96I GGNCC 2 cut(s) 1127, 1367
SchI GAGTC 4 cut(s) 70, 545, 770, 993
ScrFI CCNGG 5 cut(s) 399, 468, 993, 1014, 1410
SexAI ACCWGGT 2 cut(s) 466, 991
SfaNI GCATC 4 cut(s) 85, 226, 870, 1285
SfcI CTRYAG 1 cut(s) 774
SfuI TTCGAA 1 cut(s) 1389
SinI GGWCC 2 cut(s) 1127, 1367
SmiMI CAYNNNNRTG 3 cut(s) 575, 776, 1245
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
SpeI ACTAGT 1 cut(s) 617
SphI GCATGC 1 cut(s) 857
SsiI CCGC 4 cut(s) 63, 66, 485, 498
SspMI CTAG 2 cut(s) 305, 618
StyD4I CCNGG 5 cut(s) 397, 466, 991, 1012, 1408
StyI CCWWGG 2 cut(s) 267, 1266
TaaI ACNGT 3 cut(s) 21, 775, 1106
TaiI ACGT 1 cut(s) 986
TaqI TCGA 4 cut(s) 104, 1072, 1389, 1399
TauI GCSGC 2 cut(s) 68, 500
TfiI GAWTC 4 cut(s) 179, 959, 1333, 1394
Tru1I TTAA 5 cut(s) 23, 381, 525, 561, 1470
Tru9I TTAA 5 cut(s) 23, 381, 525, 561, 1470
TscAI CASTG 1 cut(s) 778
TseFI GTSAC 4 cut(s) 73, 242, 821, 979
TseI GCWGC 4 cut(s) 38, 198, 494, 506
Tsp45I GTSAC 4 cut(s) 73, 242, 821, 979
TspDTI ATGAA 6 cut(s) 179, 197, 681, 687, 907, 1304
TspGWI ACGGA 2 cut(s) 645, 903
TspRI CASTG 1 cut(s) 778
Van91I CCANNNNNTGG 1 cut(s) 815
Vha464I CTTAAG 1 cut(s) 560
VpaK11BI GGWCC 2 cut(s) 1127, 1367
XapI RAATTY 4 cut(s) 151, 784, 833, 1223
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 2 cut(s) 344, 857
XmiI GTMKAC 1 cut(s) 420
XspI CTAG 2 cut(s) 305, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.