pycom03g11560

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000663
Physical Location & Seq
Reverse (-)
11382461 .. 11385541
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g11560.6

Sequence Viewer

Length: 1149 bp
ATGTGTAGCAACCTTGTAAGCAGGGTGTTGATATTGGCATGGCTGTCGGCGATAACCACAGCACAAACCGTTGCTTTCCAAAACAACTACAAGGATGCTTTGTCGAAGTGCCTTTTATTTTTTGAGGGGCAAAGGTCAGGAAAGTTACCACCTTCGCAACGACTAACTTGGAGAAAAGATTCTGCCCTAAAAGATGGCTCTGATACTGGCAGGGATCTTGTAGGAGGGTACTATGATGCAGGTGACAATGTGAAGTTCAACTTTCCGATGGCGTTCACTACAACGATGTTGTCATGGAGCGTTCTAGAGTTCGGAAATTCGATGGGTGCAGATCATGAACATGCTTTGGAAGCTATAAGATGGGGGACTGACTATTTTCTTAAAGCTACTAGTGTTCCTGATTCTGTCGTTGGTGCAGTTGGTGACCCCAATGGCGATCACACTTGTTGGCAAAGGCCAGAAGACATGGACACTCCTAGAACCTCTTATGTAGTAACAAAAGAAAAGCCAGGTTCTGAGGTATCGGCCGAGATTGCAGCTGCGCTTGCAGCGTCTTCCATGGTTTTTAAAGATTCAGACAAAGACTACTCTGCTTCGCTTCTTAAACGATCCATCGAGGTTTTTGAATTCGCAGATAAGTACAGGGGCTCTTACGGTGACAGTATTGGTAGCGGAGTGTGTCCTTTTTACTGTGATTTCAGCGGCTACATGGATGAATTGGTTTGGGGAGCTGCGTGGTTATACAAGGCAACCAATACACCAGATTATTGGAACTATGTCAGAAACAACATAGTTGGTGATGTCACTGAATTTGGATGGGATTCAAAACATGCTGGCATCAACGTACTCGTTTCTCAGTGGGCAATGACTGATCCCAATAATTCTAATCCCTTTATTACCAAGGCAGACCAACTTGTATGCTCCATTTTGCCTGAATCACCTGCTCAAAAATCAGTCACATATTCACCAGGTGGGCTCTTGTTCAAACCTGGAGGAAGTAACCTCCAACATACAACGTCCCTATCATTTCTTCTTTCAGTTTATGCTGGCTACATGAAAGCTAACAACAAAGTTGTTGACTGTGGCAACAATGTTGTTGTCACTCCAGCTAAATTGGTGGATTTCACCAAAGGCCAGGTATGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

383

Amino Acids

41.85

Weight (kDa)

5.21

Isoelectric Point (pI)

28.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23210 AT1G70710
fragaria_vesca FvH4_4g33940 FvH4_6g41450 FvH4_7g27772 FvH4_7g27773 FvH4_7g27774 FvH4_7g27775
malus_domestica MD00G1036700.v1.1 MD07G1097500.v1.1 MD11G1195000.v1.1 MD16G1021500.v1.1
prunus_persica Prupe.1G331000_v2.0.a1 Prupe.1G331000_v2.0.a1 Prupe.2G121700_v2.0.a1 Prupe.2G278700_v2.0.a1 Prupe.2G278800_v2.0.a1
pyrus_communis pycom01g19480 pycom03g11560 pycom06g08760 pycom16g01920
rosa_chinensis RchiOBHm_Chr1g0374571 RchiOBHm_Chr1g0374581 RchiOBHm_Chr1g0374591 RchiOBHm_Chr1g0374601 RchiOBHm_Chr1g0374611 RchiOBHm_Chr2g0157011 RchiOBHm_Chr4g0443471
rosa_laevigata RLG00000005926 RLG00000026724 RLG00000026725 RLG00000026726 RLG00000026727
rosa_multiflora Rmu_co8114132.1_g000001 Rmu_sc0004947.1_g000005 Rmu_sc0004947.1_g000006 Rmu_sc0004947.1_g000007 Rmu_sc0004947.1_g000008 Rmu_sc0009670.1_g000003 Rmu_sc0034696.1_g000001 Rmu_sc0037664.1_g000001 Rmu_ssc0000408.1_g000014
rosa_roxburghii Rroxscaffold_4G00283360 Rroxscaffold_4G00283370 Rroxscaffold_4G00283380 Rroxscaffold_5G00384020
rosa_rugosa Rorug01G0059600 Rorug01G0384100 Rorug01G0384200 Rorug01G0384200 Rorug02G0457500 Rorug04G0346500 Rorug04G0346500 Rorug07G0215300
rosa_samantha Rh1AG395000 Rh1AG395100 Rh1AG395200 Rh1AG395400 Rh1CG372800 Rh1CG372900 Rh1CG373000 Rh1CG373100 Rh1DG390300 Rh1DG390400 Rh1DG390500 Rh1DG390600 Rh2BG536600 Rh2DG545500 Rh4AG402000 Rh4CG429100 Rh4DG409100
rosa_wichuraiana Rw1G035030 Rw1G035040 Rw1G035050 Rw1G035060 Rw4G034660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 230, 949
Acc36I ACCTGC 2 cut(s) 230, 949
AciI CCGC 2 cut(s) 672, 702
AclWI GGATC 3 cut(s) 222, 603, 866
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 3 cut(s) 316, 626, 809
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 3 cut(s) 230, 641, 846
AgsI TTSAA 4 cut(s) 259, 626, 825, 985
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 4 cut(s) 508, 967, 988, 1134
AluBI AGCT 6 cut(s) 353, 386, 539, 731, 1061, 1109
AluI AGCT 6 cut(s) 353, 386, 539, 731, 1061, 1109
AlwI GGATC 3 cut(s) 222, 603, 866
AlwNI CAGNNNCTG 1 cut(s) 515
AoxI GGCC 3 cut(s) 455, 525, 1132
ApeKI GCWGC 4 cut(s) 536, 539, 548, 731
ApoI RAATTY 3 cut(s) 316, 626, 809
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 1 cut(s) 544
AsuHPI GGTGA 7 cut(s) 254, 434, 668, 809, 930, 957, 1117
BanII GRGCYC 2 cut(s) 650, 978
BbsI GAAGAC 2 cut(s) 468, 546
BbvI GCAGC 4 cut(s) 526, 548, 560, 718
BccI CCATC 6 cut(s) 188, 262, 316, 354, 620, 810
BcgI CGANNNNNNTGC 2 cut(s) 27, 61
BciT130I CCWGG 4 cut(s) 510, 969, 990, 1136
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 3 cut(s) 305, 390, 477
BfuAI ACCTGC 2 cut(s) 230, 949
BisI GCNGC 5 cut(s) 537, 540, 549, 703, 732
BlsI GCNGC 5 cut(s) 538, 541, 550, 704, 733
Bme1390I CCNGG 4 cut(s) 510, 969, 990, 1136
BmrFI CCNGG 4 cut(s) 510, 969, 990, 1136
BmsI GCATC 3 cut(s) 85, 226, 846
BpiI GAAGAC 2 cut(s) 468, 546
BpmI CTGGAG 2 cut(s) 1011, 1089
BsaJI CCNNGG 2 cut(s) 558, 900
Bse1I ACTGG 1 cut(s) 211
Bse3DI GCAATG 1 cut(s) 870
BseBI CCWGG 4 cut(s) 510, 969, 990, 1136
BseDI CCNNGG 2 cut(s) 558, 900
BseGI GGATG 3 cut(s) 100, 718, 821
BseMI GCAATG 1 cut(s) 870
BseMII CTCAG 2 cut(s) 507, 869
BseNI ACTGG 1 cut(s) 211
BseX3I CGGCCG 1 cut(s) 525
BseXI GCAGC 4 cut(s) 526, 548, 560, 718
BsgI GTGCAG 2 cut(s) 348, 435
Bsh1285I CGRYCG 1 cut(s) 528
BshFI GGCC 3 cut(s) 457, 527, 1134
BsiEI CGRYCG 1 cut(s) 528
BslFI GGGAC 2 cut(s) 379, 1003
BsmFI GGGAC 2 cut(s) 379, 1003
BsnI GGCC 3 cut(s) 457, 527, 1134
Bsp1286I GDGCHC 2 cut(s) 650, 978
Bsp143I GATC 5 cut(s) 214, 331, 436, 608, 871
Bsp19I CCATGG 1 cut(s) 558
BspACI CCGC 2 cut(s) 672, 702
BspANI GGCC 3 cut(s) 457, 527, 1134
BspCNI CTCAG 2 cut(s) 508, 868
BspHI TCATGA 1 cut(s) 334
BspMI ACCTGC 2 cut(s) 230, 949
BspPI GGATC 3 cut(s) 222, 603, 866
BsrDI GCAATG 1 cut(s) 870
BsrI ACTGG 1 cut(s) 211
BssECI CCNNGG 2 cut(s) 558, 900
BssMI GATC 5 cut(s) 214, 331, 436, 608, 871
BssT1I CCWWGG 2 cut(s) 558, 900
Bst2UI CCWGG 4 cut(s) 510, 969, 990, 1136
Bst4CI ACNGT 5 cut(s) 70, 656, 662, 692, 1082
BstC8I GCNNGC 3 cut(s) 546, 835, 1048
BstDEI CTNAG 2 cut(s) 516, 855
BstDSI CCRYGG 1 cut(s) 558
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 3 cut(s) 100, 718, 821
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 5 cut(s) 217, 334, 439, 611, 874
BstMBI GATC 5 cut(s) 214, 331, 436, 608, 871
BstMCI CGRYCG 1 cut(s) 528
BstMWI GCNNNNNNNGC 4 cut(s) 350, 533, 545, 548
BstNI CCWGG 4 cut(s) 510, 969, 990, 1136
BstNSI RCATGY 2 cut(s) 344, 833
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 4 cut(s) 508, 967, 988, 1134
BstV1I GCAGC 4 cut(s) 526, 548, 560, 718
BstV2I GAAGAC 2 cut(s) 468, 546
BstX2I RGATCY 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 768
BstYI RGATCY 1 cut(s) 214
BstZI CGGCCG 1 cut(s) 525
BsuRI GGCC 3 cut(s) 457, 527, 1134
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 3 cut(s) 100, 718, 821
BtsIMutI CAGTG 2 cut(s) 804, 863
BveI ACCTGC 2 cut(s) 230, 949
Cac8I GCNNGC 3 cut(s) 546, 835, 1048
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 544
CseI GACGC 1 cut(s) 540
CsiI ACCWGGT 1 cut(s) 967
Csp6I GTAC 3 cut(s) 229, 640, 845
CviAII CATG 9 cut(s) 39, 294, 335, 341, 466, 559, 709, 830, 1054
CviQI GTAC 3 cut(s) 229, 640, 845
DdeI CTNAG 2 cut(s) 516, 855
DpnI GATC 5 cut(s) 216, 333, 438, 610, 873
DpnII GATC 5 cut(s) 214, 331, 436, 608, 871
DraI TTTAAA 1 cut(s) 568
DraIII CACNNNGTG 1 cut(s) 971
EaeI YGGCCR 1 cut(s) 525
EagI CGGCCG 1 cut(s) 525
EclXI CGGCCG 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 558, 900
Eco24I GRGCYC 2 cut(s) 650, 978
Eco52I CGGCCG 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 422
EcoO65I GGTNACC 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 4 cut(s) 508, 967, 988, 1134
EcoT14I CCWWGG 2 cut(s) 558, 900
EcoT38I GRGCYC 2 cut(s) 650, 978
ErhI CCWWGG 2 cut(s) 558, 900
FaeI CATG 9 cut(s) 42, 297, 338, 344, 469, 562, 712, 833, 1057
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 2 cut(s) 379, 1003
FatI CATG 9 cut(s) 38, 293, 334, 340, 465, 558, 708, 829, 1053
Fnu4HI GCNGC 5 cut(s) 537, 540, 549, 703, 732
FokI GGATG 3 cut(s) 107, 725, 828
FriOI GRGCYC 2 cut(s) 650, 978
Fsp4HI GCNGC 5 cut(s) 537, 540, 549, 703, 732
FspBI CTAG 3 cut(s) 305, 390, 477
GlaI GCGC 1 cut(s) 543
GluI GCNGC 5 cut(s) 537, 540, 549, 703, 732
GsuI CTGGAG 2 cut(s) 1011, 1089
HaeIII GGCC 3 cut(s) 457, 527, 1134
HgaI GACGC 1 cut(s) 540
HhaI GCGC 1 cut(s) 544
Hin1II CATG 9 cut(s) 42, 297, 338, 344, 469, 562, 712, 833, 1057
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 1 cut(s) 1078
HindII GTYRAC 1 cut(s) 1078
HinfI GANTC 5 cut(s) 179, 401, 572, 821, 935
HphI GGTGA 7 cut(s) 254, 434, 668, 809, 930, 957, 1117
Hpy166II GTNNAC 2 cut(s) 276, 1078
Hpy188I TCNGA 6 cut(s) 202, 267, 314, 517, 577, 782
Hpy188III TCNNGA 4 cut(s) 138, 305, 335, 398
Hpy8I GTNNAC 2 cut(s) 276, 1078
HpyAV CCTTC 1 cut(s) 162
HpyCH4III ACNGT 5 cut(s) 70, 656, 662, 692, 1082
HpyCH4IV ACGT 2 cut(s) 843, 1016
HpyCH4V TGCA 5 cut(s) 239, 329, 416, 536, 548
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 533, 545, 548
HpyF3I CTNAG 2 cut(s) 516, 855
HpySE526I ACGT 2 cut(s) 843, 1016
Hsp92II CATG 9 cut(s) 42, 297, 338, 344, 469, 562, 712, 833, 1057
HspAI GCGC 1 cut(s) 542
Kzo9I GATC 5 cut(s) 214, 331, 436, 608, 871
LmnI GCTCC 3 cut(s) 297, 728, 926
Lsp1109I GCAGC 4 cut(s) 526, 548, 560, 718
LweI GCATC 3 cut(s) 85, 226, 846
MabI ACCWGGT 1 cut(s) 967
MaeI CTAG 3 cut(s) 305, 390, 477
MaeII ACGT 2 cut(s) 843, 1016
MaeIII GTNAC 9 cut(s) 144, 242, 422, 493, 656, 802, 955, 998, 1099
MalI GATC 5 cut(s) 216, 333, 438, 610, 873
MboI GATC 5 cut(s) 214, 331, 436, 608, 871
MboII GAAGA 3 cut(s) 473, 546, 1022
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 650, 978
MluCI AATT 6 cut(s) 316, 626, 716, 809, 880, 1112
MmeI TCCRAC 1 cut(s) 1030
MnlI CCTC 7 cut(s) 118, 218, 493, 511, 610, 986, 1013
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 3 cut(s) 381, 567, 603
MslI CAYNNNNRTG 1 cut(s) 339
MspA1I CMGCKG 2 cut(s) 539, 702
MspR9I CCNGG 4 cut(s) 510, 969, 990, 1136
MvaI CCWGG 4 cut(s) 510, 969, 990, 1136
MwoI GCNNNNNNNGC 4 cut(s) 350, 533, 545, 548
NcoI CCATGG 1 cut(s) 558
NdeII GATC 5 cut(s) 214, 331, 436, 608, 871
NlaIII CATG 9 cut(s) 42, 297, 338, 344, 469, 562, 712, 833, 1057
NmeAIII GCCGAG 1 cut(s) 553
NmuCI GTSAC 6 cut(s) 242, 422, 656, 802, 955, 1099
NspI RCATGY 2 cut(s) 344, 833
PagI TCATGA 1 cut(s) 334
PaqCI CACCTGC 2 cut(s) 230, 949
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 5 cut(s) 179, 401, 572, 821, 935
PkrI GCNGC 5 cut(s) 538, 541, 550, 704, 733
Psp6I CCWGG 4 cut(s) 508, 967, 988, 1134
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 4 cut(s) 508, 967, 988, 1134
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 3 cut(s) 230, 641, 846
RsaNI GTAC 3 cut(s) 229, 640, 845
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 3 cut(s) 381, 567, 603
SatI GCNGC 5 cut(s) 537, 540, 549, 703, 732
Sau3AI GATC 5 cut(s) 214, 331, 436, 608, 871
ScrFI CCNGG 4 cut(s) 510, 969, 990, 1136
SduI GDGCHC 2 cut(s) 650, 978
SexAI ACCWGGT 1 cut(s) 967
SfaNI GCATC 3 cut(s) 85, 226, 846
SmiMI CAYNNNNRTG 1 cut(s) 339
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 6 cut(s) 316, 626, 716, 809, 880, 1112
SsiI CCGC 2 cut(s) 672, 702
SspMI CTAG 3 cut(s) 305, 390, 477
StyD4I CCNGG 4 cut(s) 508, 967, 988, 1134
StyI CCWWGG 2 cut(s) 558, 900
TaaI ACNGT 5 cut(s) 70, 656, 662, 692, 1082
TaiI ACGT 2 cut(s) 846, 1019
TaqI TCGA 3 cut(s) 104, 320, 615
TasI AATT 6 cut(s) 316, 626, 716, 809, 880, 1112
TatI WGTACW 1 cut(s) 639
TauI GCSGC 1 cut(s) 705
TfiI GAWTC 5 cut(s) 179, 401, 572, 821, 935
Tru1I TTAA 3 cut(s) 381, 567, 603
Tru9I TTAA 3 cut(s) 381, 567, 603
TscAI CASTG 2 cut(s) 811, 863
TseFI GTSAC 6 cut(s) 242, 422, 656, 802, 955, 1099
TseI GCWGC 4 cut(s) 536, 539, 548, 731
Tsp45I GTSAC 6 cut(s) 242, 422, 656, 802, 955, 1099
TspDTI ATGAA 3 cut(s) 351, 729, 1070
TspRI CASTG 2 cut(s) 811, 863
XapI RAATTY 3 cut(s) 316, 626, 809
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 2 cut(s) 344, 833
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 3 cut(s) 305, 390, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.