MD01G1216900.v1.1

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000755
Physical Location & Seq
Reverse (-)
30966831 .. 30969425
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1216900.v1.1.491

Sequence Viewer

Length: 1773 bp
ATGGCCATGGTGGTTCAAATTCCGCGGGTACATCACTGTGACTTAGAAAAAAATGCTGCAGAAAAATTCCCAAAGAATATTAGGGAAGCACAGAAAAAATTCCCCATGAAAAAGCGTCTTGATTTACGGGAGTTCGTCCTGCAGAGGTGGAATCAGATACTTTTAGTTTTCTGTGTTATCGCTCTCTCAACCGATCCTTTGTTCTTTTACCTCCTTACGGTTAACAACGAGCAGAATTGCCTTACCCTGGACAAAACATTGGGGACCGTAACTGCGCTTCTGCGTTTCAGCATCGATTTCTTTTATATATTTCATATCATTTTTCAATACCGTGCAAGCATTGTTGCTCATTCTTTTCAAGCCTCTCGACAAGGCGAATCGGTTGCAGAGACTCGGGCAAGAGCACGGAAATATTTGTCGTTGTACCTGCTACTCGACGGGCTTATGATTCTTCCATTTCCACAGGAGCTAGTTTTTGCTGTCTTTCCAAAACGAAATAACTCGAGCGTTTCCGGTGAAACAAATCTATTGATCTTGATGTTTATGTTTCAATATCTGCTAAGGGCTTTTCGAATCGTCTCTTTGTTAAAAGTATCTCTAAGGGGTTCTAGCATCTTCGCCGAATCTGCATGGCCTGATCTCTTTCTTTTTATGCTTGCGGGTCATGTTGCTGGAGCCTTTTGGTATTTTTTCTCTTTAGGAAGAATAGCAACGTGCTGGCGACAAGTCTGTGAATCGCATGGAGATGCGCATTGTTATTTATATTGCGATGACACATTTGAAGCTAAAGCATTCGCATACGACTCTTGCTCTACAGACACAATGAAATTTGGAATATACTCTGATGCCCTTAAATTTGTAGTTCTGGATTCAACTCCTTTTGTAAAAAGGATTTCTTACTGCTTCTGGTGGGGGATACAAAATCTAAGTTCCCTCGGCCAAGGCCTCAAGCCCGCTACAATCGACGTATGGGAGGTATACTTTTCACTACTTGTTTCGATATCTGGCTTGGTGGTAATTGCAATTCTTGCTGGAAATCTGCAGAAATATTTGCTGTCAAAAGTTGCGAGATCGGAGGAAATGAGATCGAAGGAACAAGAAATAGAATTGTGGATGTATTACCATTCACTTCCGCGGAGTCTTAAGGCGCGGATTTGGCAATCCATGAAACACAAACTGAAAGAAAACATACGCAATGAGAATTTTCTTCACCTTCTTCCCGTGGAACTTTGTAAGGATATAAAATGGCATCTCTGCTCAGGTCCACTCAGAGAAGTGCCAATACTTCAAACCATGGACGAGCAACTGTTGTTCGCCATCTGCAAACATCTCACGCCGGTGCTGTACGCGAAGAACAGCATCATTTTTCATGAGGGAGAGCCACTTGCTGAGATGCTCTTCGTCACATGCGGTAAGCTATTGACTTACACCGCCTCTGCTACTGCTAGGGTAAGATTTCTTGGCAAAGGTGACTTCTATGGAGAAGAGCTTTTCGATTGGGTGCTGATCCACCTTGCCAGCTTATCTAACTTCCCCTTGTCAACTGAAACTGTCAAGGCGCAAACGGAAGTTGAAGTGTTTGTTCTAAGGGCGCAGCAATTGAAGACGGTAGTCTTTAAATTCTGGTGGCTTTTCACCAAAGAGCAACGCCACCACTCGAGCCTTAATACTAGCTTTAACGTGGAGCAATGGAAACCAAGGGCAGCTTATGCTTTGCAAGCAGCTTGGCGTCGCCATAAAAAGGAAACCTCGACGGTGCGAAGGACAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

591

Amino Acids

68.43

Weight (kDa)

9.17

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 448 - 538 2.3e-09 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 435
AccI GTMKAC 1 cut(s) 978
AccII CGCG 4 cut(s) 25, 1135, 1150, 1349
AciI CCGC 9 cut(s) 23, 25, 659, 954, 1133, 1135, 1150, 1410, 1431
AclWI GGATC 2 cut(s) 188, 1501
AcoI YGGCCR 2 cut(s) 3, 937
AcsI RAATTY 7 cut(s) 18, 65, 98, 827, 854, 1201, 1619
AcyI GRCGYC 1 cut(s) 1729
AfaI GTAC 3 cut(s) 30, 425, 1346
AfiI CCNNNNNNNGG 3 cut(s) 217, 247, 1741
AflII CTTAAG 1 cut(s) 1142
AgsI TTSAA 9 cut(s) 17, 326, 359, 551, 782, 873, 1289, 1574, 1603
AjnI CCWGG 1 cut(s) 246
AleI CACNNNNGTG 1 cut(s) 1337
AluBI AGCT 8 cut(s) 469, 785, 1417, 1489, 1521, 1674, 1706, 1724
AluI AGCT 8 cut(s) 469, 785, 1417, 1489, 1521, 1674, 1706, 1724
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 2 cut(s) 383, 583
AlwI GGATC 2 cut(s) 188, 1501
Ama87I CYCGRG 3 cut(s) 393, 502, 1657
AoxI GGCC 4 cut(s) 3, 632, 937, 943
ApeKI GCWGC 4 cut(s) 56, 1594, 1703, 1721
ApoI RAATTY 7 cut(s) 18, 65, 98, 827, 854, 1201, 1619
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 5 cut(s) 277, 751, 1150, 1561, 1594
AspS9I GGNCC 2 cut(s) 264, 1262
AsuHPI GGTGA 4 cut(s) 527, 1202, 1481, 1627
AsuII TTCGAA 1 cut(s) 571
AvaI CYCGRG 3 cut(s) 393, 502, 1657
AvaII GGWCC 2 cut(s) 264, 1262
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 4 cut(s) 261, 293, 1266, 1298
BbsI GAAGAC 1 cut(s) 1610
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 4 cut(s) 43, 1606, 1715, 1733
BccI CCATC 1 cut(s) 1325
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 1 cut(s) 248
BciVI GTATCC 1 cut(s) 909
BcoDI GTCTC 2 cut(s) 383, 583
BfaI CTAG 4 cut(s) 470, 609, 1446, 1671
BfmI CTRYAG 4 cut(s) 57, 140, 813, 1040
BfrI CTTAAG 1 cut(s) 1142
BfuAI ACCTGC 1 cut(s) 435
BfuI GTATCC 1 cut(s) 909
BisI GCNGC 4 cut(s) 57, 1595, 1704, 1722
BlsI GCNGC 4 cut(s) 58, 1596, 1705, 1723
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 2 cut(s) 264, 1262
BmeT110I CYCGRG 3 cut(s) 393, 502, 1657
BmgT120I GGNCC 2 cut(s) 264, 1262
BmiI GGNNCC 2 cut(s) 265, 676
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 7 cut(s) 300, 621, 736, 835, 1258, 1368, 1383
BoxI GACNNNNGTC 1 cut(s) 1610
BpiI GAAGAC 1 cut(s) 1610
BpmI CTGGAG 1 cut(s) 693
Bpu10I CCTNAGC 2 cut(s) 560, 1258
Bpu14I TTCGAA 1 cut(s) 571
BpuEI CTTGAG 1 cut(s) 932
Bsa29I ATCGAT 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 1729
BsaJI CCNNGG 9 cut(s) 6, 23, 246, 934, 940, 1133, 1221, 1293, 1697
BsaWI WCCGGW 1 cut(s) 512
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 247, 1741
Bse118I RCCGGY 1 cut(s) 1336
Bse3DI GCAATG 2 cut(s) 1201, 1694
BseBI CCWGG 1 cut(s) 248
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 9 cut(s) 6, 23, 246, 934, 940, 1133, 1221, 1293, 1697
BseGI GGATG 1 cut(s) 1119
BseLI CCNNNNNNNGG 3 cut(s) 217, 247, 1741
BseMI GCAATG 2 cut(s) 1201, 1694
BseMII CTCAG 3 cut(s) 1272, 1282, 1380
BseXI GCAGC 4 cut(s) 43, 1606, 1715, 1733
Bsh1236I CGCG 4 cut(s) 25, 1135, 1150, 1349
BshFI GGCC 4 cut(s) 5, 634, 939, 945
BshVI ATCGAT 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 3 cut(s) 393, 502, 1657
BsiSI CCGG 2 cut(s) 513, 1337
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 3 cut(s) 217, 247, 1741
BsmAI GTCTC 2 cut(s) 383, 583
BsmBI CGTCTC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 791
BsnI GGCC 4 cut(s) 5, 634, 939, 945
BsoBI CYCGRG 3 cut(s) 393, 502, 1657
Bsp119I TTCGAA 1 cut(s) 571
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 6 cut(s) 193, 531, 637, 1070, 1085, 1506
Bsp19I CCATGG 2 cut(s) 6, 1293
BspACI CCGC 9 cut(s) 23, 25, 659, 954, 1133, 1135, 1150, 1410, 1431
BspANI GGCC 4 cut(s) 5, 634, 939, 945
BspCNI CTCAG 3 cut(s) 1271, 1281, 1381
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 4 cut(s) 25, 1135, 1150, 1349
BspHI TCATGA 1 cut(s) 1369
BspLI GGNNCC 2 cut(s) 265, 676
BspMAI CTGCAG 3 cut(s) 61, 144, 1044
BspMI ACCTGC 1 cut(s) 435
BspPI GGATC 2 cut(s) 188, 1501
BspQI GCTCTTC 2 cut(s) 1403, 1479
BspT104I TTCGAA 1 cut(s) 571
BspTI CTTAAG 1 cut(s) 1142
BsrDI GCAATG 2 cut(s) 1201, 1694
BsrFI RCCGGY 1 cut(s) 1336
BssAI RCCGGY 1 cut(s) 1336
BssECI CCNNGG 9 cut(s) 6, 23, 246, 934, 940, 1133, 1221, 1293, 1697
BssMI GATC 6 cut(s) 193, 531, 637, 1070, 1085, 1506
BssNAI GTATAC 1 cut(s) 979
BssNI GRCGYC 1 cut(s) 1729
BssT1I CCWWGG 4 cut(s) 6, 940, 1293, 1697
Bst1107I GTATAC 1 cut(s) 979
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 8 cut(s) 38, 220, 268, 332, 1308, 1552, 1609, 1756
Bst6I CTCTTC 2 cut(s) 1403, 1479
BstACI GRCGYC 1 cut(s) 1729
BstAFI CTTAAG 1 cut(s) 1142
BstAPI GCANNNNNTGC 2 cut(s) 1028, 1709
BstBI TTCGAA 1 cut(s) 571
BstC8I GCNNGC 6 cut(s) 337, 657, 719, 954, 1519, 1719
BstDEI CTNAG 8 cut(s) 43, 560, 599, 926, 1258, 1268, 1389, 1586
BstDSI CCRYGG 5 cut(s) 6, 23, 1133, 1221, 1293
BstF5I GGATG 1 cut(s) 1119
BstFNI CGCG 4 cut(s) 25, 1135, 1150, 1349
BstHHI GCGC 5 cut(s) 277, 751, 1150, 1561, 1594
BstKTI GATC 6 cut(s) 196, 534, 640, 1073, 1088, 1509
BstMAI GTCTC 2 cut(s) 383, 583
BstMBI GATC 6 cut(s) 193, 531, 637, 1070, 1085, 1506
BstMWI GCNNNNNNNGC 5 cut(s) 626, 1028, 1156, 1709, 1718
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 1410
BstPAI GACNNNNGTC 1 cut(s) 1610
BstSCI CCNGG 1 cut(s) 246
BstSFI CTRYAG 4 cut(s) 57, 140, 813, 1040
BstUI CGCG 4 cut(s) 25, 1135, 1150, 1349
BstV1I GCAGC 4 cut(s) 43, 1606, 1715, 1733
BstV2I GAAGAC 1 cut(s) 1610
BstZ17I GTATAC 1 cut(s) 979
Bsu15I ATCGAT 1 cut(s) 294
BsuI GTATCC 1 cut(s) 909
BsuRI GGCC 4 cut(s) 5, 634, 939, 945
BsuTUI ATCGAT 1 cut(s) 294
BtgI CCRYGG 5 cut(s) 6, 23, 1133, 1221, 1293
BtgZI GCGATG 1 cut(s) 783
BtsCI GGATG 1 cut(s) 1119
BtsIMutI CAGTG 1 cut(s) 34
BveI ACCTGC 1 cut(s) 435
Cac8I GCNNGC 6 cut(s) 337, 657, 719, 954, 1519, 1719
CciI TCATGA 1 cut(s) 1369
CfoI GCGC 5 cut(s) 277, 751, 1150, 1561, 1594
Cfr10I RCCGGY 1 cut(s) 1336
Cfr13I GGNCC 2 cut(s) 264, 1262
Cfr42I CCGCGG 2 cut(s) 26, 1136
ClaI ATCGAT 1 cut(s) 294
CseI GACGC 2 cut(s) 104, 1718
Csp6I GTAC 3 cut(s) 29, 424, 1345
CviAII CATG 9 cut(s) 7, 106, 630, 665, 740, 1165, 1294, 1370, 1407
CviQI GTAC 3 cut(s) 29, 424, 1345
DdeI CTNAG 8 cut(s) 43, 560, 599, 926, 1258, 1268, 1389, 1586
DpnI GATC 6 cut(s) 195, 533, 639, 1072, 1087, 1508
DpnII GATC 6 cut(s) 193, 531, 637, 1070, 1085, 1506
DraI TTTAAA 1 cut(s) 1618
EaeI YGGCCR 2 cut(s) 3, 937
Eam1104I CTCTTC 2 cut(s) 1403, 1479
EarI CTCTTC 2 cut(s) 1403, 1479
Eco130I CCWWGG 4 cut(s) 6, 940, 1293, 1697
Eco147I AGGCCT 1 cut(s) 945
Eco32I GATATC 1 cut(s) 1002
Eco47I GGWCC 2 cut(s) 264, 1262
Eco88I CYCGRG 3 cut(s) 393, 502, 1657
EcoRII CCWGG 1 cut(s) 246
EcoRV GATATC 1 cut(s) 1002
EcoT14I CCWWGG 4 cut(s) 6, 940, 1293, 1697
ErhI CCWWGG 4 cut(s) 6, 940, 1293, 1697
Esp3I CGTCTC 1 cut(s) 583
FaeI CATG 9 cut(s) 10, 109, 633, 668, 743, 1168, 1297, 1373, 1410
FalI AAGNNNNNCTT 4 cut(s) 880, 912, 1690, 1722
FaqI GGGAC 1 cut(s) 277
FatI CATG 9 cut(s) 6, 105, 629, 664, 739, 1164, 1293, 1369, 1406
FauI CCCGC 3 cut(s) 18, 652, 961
FblI GTMKAC 1 cut(s) 978
Fnu4HI GCNGC 4 cut(s) 57, 1595, 1704, 1722
FokI GGATG 1 cut(s) 1126
Fsp4HI GCNGC 4 cut(s) 57, 1595, 1704, 1722
FspAI RTGCGCAY 1 cut(s) 750
FspBI CTAG 4 cut(s) 470, 609, 1446, 1671
FspI TGCGCA 1 cut(s) 750
GlaI GCGC 5 cut(s) 276, 750, 1149, 1560, 1593
GluI GCNGC 4 cut(s) 57, 1595, 1704, 1722
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 4 cut(s) 5, 634, 939, 945
HapII CCGG 2 cut(s) 513, 1337
HgaI GACGC 2 cut(s) 104, 1718
HhaI GCGC 5 cut(s) 277, 751, 1150, 1561, 1594
Hin1I GRCGYC 1 cut(s) 1729
Hin1II CATG 9 cut(s) 10, 109, 633, 668, 743, 1168, 1297, 1373, 1410
Hin6I GCGC 5 cut(s) 275, 749, 1148, 1559, 1592
HinP1I GCGC 5 cut(s) 275, 749, 1148, 1559, 1592
HincII GTYRAC 2 cut(s) 223, 1542
HindII GTYRAC 2 cut(s) 223, 1542
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 2 cut(s) 513, 1337
HphI GGTGA 4 cut(s) 527, 1202, 1481, 1627
Hpy166II GTNNAC 4 cut(s) 223, 979, 1265, 1542
Hpy188I TCNGA 4 cut(s) 156, 844, 1075, 1271
Hpy188III TCNNGA 5 cut(s) 119, 366, 535, 866, 1370
Hpy8I GTNNAC 4 cut(s) 223, 979, 1265, 1542
Hpy99I CGWCG 4 cut(s) 440, 968, 1734, 1756
HpyAV CCTTC 3 cut(s) 1084, 1223, 1755
HpyCH4III ACNGT 8 cut(s) 38, 220, 268, 332, 1308, 1552, 1609, 1756
HpyCH4IV ACGT 3 cut(s) 713, 966, 1680
HpyCH4V TGCA 9 cut(s) 59, 142, 335, 386, 629, 1022, 1042, 1323, 1717
HpyF10VI GCNNNNNNNGC 5 cut(s) 626, 1028, 1156, 1709, 1718
HpyF3I CTNAG 8 cut(s) 43, 560, 599, 926, 1258, 1268, 1389, 1586
HpySE526I ACGT 3 cut(s) 713, 966, 1680
Hsp92I GRCGYC 1 cut(s) 1729
Hsp92II CATG 9 cut(s) 10, 109, 633, 668, 743, 1168, 1297, 1373, 1410
HspAI GCGC 5 cut(s) 275, 749, 1148, 1559, 1592
KspAI GTTAAC 1 cut(s) 223
KspI CCGCGG 2 cut(s) 26, 1136
Kzo9I GATC 6 cut(s) 193, 531, 637, 1070, 1085, 1506
LguI GCTCTTC 2 cut(s) 1403, 1479
LmnI GCTCC 3 cut(s) 466, 674, 1684
Lsp1109I GCAGC 4 cut(s) 43, 1606, 1715, 1733
LweI GCATC 7 cut(s) 300, 621, 736, 835, 1258, 1368, 1383
MaeI CTAG 4 cut(s) 470, 609, 1446, 1671
MaeII ACGT 3 cut(s) 713, 966, 1680
MaeIII GTNAC 4 cut(s) 38, 268, 1402, 1469
MalI GATC 6 cut(s) 195, 533, 639, 1072, 1087, 1508
MboI GATC 6 cut(s) 193, 531, 637, 1070, 1085, 1506
MboII GAAGA 9 cut(s) 443, 607, 714, 1199, 1208, 1363, 1390, 1496, 1615
MfeI CAATTG 1 cut(s) 1598
MhlI GDGCHC 1 cut(s) 406
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 385, 797, 1147
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 222, 587, 852, 1143, 1617, 1665, 1677, 1771
MslI CAYNNNNRTG 3 cut(s) 36, 744, 1337
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 25, 1135
MspCI CTTAAG 1 cut(s) 1142
MspI CCGG 2 cut(s) 513, 1337
MspR9I CCNGG 1 cut(s) 248
MunI CAATTG 1 cut(s) 1598
Mva1269I GAATGC 1 cut(s) 791
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 4 cut(s) 25, 1135, 1150, 1349
MwoI GCNNNNNNNGC 5 cut(s) 626, 1028, 1156, 1709, 1718
NcoI CCATGG 2 cut(s) 6, 1293
NdeII GATC 6 cut(s) 193, 531, 637, 1070, 1085, 1506
NlaIII CATG 9 cut(s) 10, 109, 633, 668, 743, 1168, 1297, 1373, 1410
NlaIV GGNNCC 2 cut(s) 265, 676
NmeAIII GCCGAG 1 cut(s) 915
NmuCI GTSAC 3 cut(s) 38, 1402, 1469
NsbI TGCGCA 1 cut(s) 750
NspI RCATGY 1 cut(s) 1410
NspV TTCGAA 1 cut(s) 571
OliI CACNNNNGTG 1 cut(s) 1337
PaeR7I CTCGAG 2 cut(s) 502, 1657
PagI TCATGA 1 cut(s) 1369
PceI AGGCCT 1 cut(s) 945
PciSI GCTCTTC 2 cut(s) 1403, 1479
PctI GAATGC 1 cut(s) 791
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 7 cut(s) 151, 377, 448, 573, 623, 734, 869
PkrI GCNGC 4 cut(s) 58, 1596, 1705, 1723
PleI GAGTC 3 cut(s) 385, 797, 1146
PpsI GAGTC 3 cut(s) 385, 797, 1146
PshAI GACNNNNGTC 1 cut(s) 1610
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 265, 676
PspPI GGNCC 2 cut(s) 264, 1262
PspXI VCTCGAGB 2 cut(s) 502, 1657
PstI CTGCAG 3 cut(s) 61, 144, 1044
RsaI GTAC 3 cut(s) 30, 425, 1346
RsaNI GTAC 3 cut(s) 29, 424, 1345
RseI CAYNNNNRTG 3 cut(s) 36, 744, 1337
SacII CCGCGG 2 cut(s) 26, 1136
SapI GCTCTTC 2 cut(s) 1403, 1479
SaqAI TTAA 8 cut(s) 222, 587, 852, 1143, 1617, 1665, 1677, 1771
SatI GCNGC 4 cut(s) 57, 1595, 1704, 1722
Sau3AI GATC 6 cut(s) 193, 531, 637, 1070, 1085, 1506
Sau96I GGNCC 2 cut(s) 264, 1262
SchI GAGTC 3 cut(s) 385, 797, 1147
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 1 cut(s) 406
SfaNI GCATC 7 cut(s) 300, 621, 736, 835, 1258, 1368, 1383
SfcI CTRYAG 4 cut(s) 57, 140, 813, 1040
Sfr274I CTCGAG 2 cut(s) 502, 1657
Sfr303I CCGCGG 2 cut(s) 26, 1136
SfuI TTCGAA 1 cut(s) 571
SgrAI CRCCGGYG 1 cut(s) 1336
SgrBI CCGCGG 2 cut(s) 26, 1136
SinI GGWCC 2 cut(s) 264, 1262
SlaI CTCGAG 2 cut(s) 502, 1657
SmiMI CAYNNNNRTG 3 cut(s) 36, 744, 1337
SmlI CTYRAG 4 cut(s) 502, 947, 1142, 1657
SmoI CTYRAG 4 cut(s) 502, 947, 1142, 1657
SseBI AGGCCT 1 cut(s) 945
SsiI CCGC 9 cut(s) 23, 25, 659, 954, 1133, 1135, 1150, 1410, 1431
SspI AATATT 3 cut(s) 79, 413, 1049
SspMI CTAG 4 cut(s) 470, 609, 1446, 1671
StuI AGGCCT 1 cut(s) 945
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 4 cut(s) 6, 940, 1293, 1697
TaaI ACNGT 8 cut(s) 38, 220, 268, 332, 1308, 1552, 1609, 1756
TaiI ACGT 3 cut(s) 716, 969, 1683
TfiI GAWTC 7 cut(s) 151, 377, 448, 573, 623, 734, 869
Tru1I TTAA 8 cut(s) 222, 587, 852, 1143, 1617, 1665, 1677, 1771
Tru9I TTAA 8 cut(s) 222, 587, 852, 1143, 1617, 1665, 1677, 1771
TscAI CASTG 1 cut(s) 41
TseFI GTSAC 3 cut(s) 38, 1402, 1469
TseI GCWGC 4 cut(s) 56, 1594, 1703, 1721
Tsp45I GTSAC 3 cut(s) 38, 1402, 1469
TspDTI ATGAA 5 cut(s) 122, 302, 839, 1181, 1358
TspGWI ACGGA 2 cut(s) 421, 1580
TspRI CASTG 1 cut(s) 41
Vha464I CTTAAG 1 cut(s) 1142
VpaK11BI GGWCC 2 cut(s) 264, 1262
XapI RAATTY 7 cut(s) 18, 65, 98, 827, 854, 1201, 1619
XceI RCATGY 1 cut(s) 1410
XhoI CTCGAG 2 cut(s) 502, 1657
XmiI GTMKAC 1 cut(s) 978
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 4 cut(s) 470, 609, 1446, 1671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.