pycom01g22740

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000755
Physical Location & Seq
Reverse (-)
20245389 .. 20246597
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22740.4

Sequence Viewer

Length: 804 bp
ATGTACCTCCTTACGGTTAACAACGAACAGAATTGCCTTACCCTGGACAAAACATTGGCGACCGTAACTGCGCTTCTGCGTTTCAGCATCGATTTCTTTTATATATTTCATATCATTTTTCAATTCCGTGCAAGCAATGTTGCTCATTCTTTTCAAACCTCTCGACAAGGCGAATCGGTTGCAGAGACTCGGGCAAGAGCACGGAAATATTTGTTGATGTACCTACTAGTCGACATGCTTATAATTCTTCCACTTCCACAGGTGCTAATTTTTGCTGTCATTTCAAAAATTAAACATTCGAGGGCTTTCTTTGTCGCAAGTATATGGAAAAATATGTTTGTCCTTCAATATCTGCTAAGGTTTTTTCGAATGGGCTTTTCGTTAAAAGTAGCTCTAAGGGGTTCTAGCATCCTTGCCGAATCTGCATGGGCTGATCTCTTTCTTTTTATGCTTGCCGGTCATGTTACTGGAGCCTTCTGGTATTTTTTTTCTATAAAAAGAATAACGAAGTGCTGGGGAGAAGCCTGCGAATATCATGGAGGTGCGCACTGCTATTTATTTTGCGACGACACATTTGCAGCTAACGCACCCGTATACAACTCTTGCTCTGCAAACACAATGAATTTTGGAATATACTATGATGCCCTTGAATCTATAGTTCTGGATTCAACTCCTTTTGTAAAAAGGATTTCTTACTGCTTCTGGTGGGGGATACAAAATCTAAGTGCAGCTCCCTCGGCCAAAGCCTCAAACCCACAACTATCTACGTATGGGAGATATACTTTTCACTTGCTGTTTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

268

Amino Acids

30.7

Weight (kDa)

9.24

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 242
Acc16I TGCGCA 1 cut(s) 546
AccI GTMKAC 2 cut(s) 231, 594
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 1 cut(s) 622
AfaI GTAC 2 cut(s) 5, 221
AfiI CCNNNNNNNGG 2 cut(s) 13, 43
AgsI TTSAA 6 cut(s) 122, 155, 285, 347, 650, 669
AhlI ACTAGT 1 cut(s) 226
AjnI CCWGG 1 cut(s) 42
AluBI AGCT 3 cut(s) 392, 581, 731
AluI AGCT 3 cut(s) 392, 581, 731
Alw21I GWGCWC 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 179
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 1 cut(s) 738
ApeKI GCWGC 2 cut(s) 578, 728
ApoI RAATTY 1 cut(s) 622
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
AspLEI GCGC 2 cut(s) 73, 547
AsuII TTCGAA 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 189
BarI GAAGNNNNNNTAC 2 cut(s) 57, 89
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 2 cut(s) 590, 740
BcgI CGANNNNNNTGC 6 cut(s) 161, 195, 557, 591, 717, 751
BciT130I CCWGG 1 cut(s) 44
BciVI GTATCC 1 cut(s) 705
BcoDI GTCTC 1 cut(s) 179
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 2 cut(s) 227, 405
BfmI CTRYAG 1 cut(s) 654
BfuI GTATCC 1 cut(s) 705
BisI GCNGC 2 cut(s) 579, 729
BlsI GCNGC 2 cut(s) 580, 730
Bme1390I CCNGG 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 44
BmsI GCATC 3 cut(s) 96, 417, 631
BpmI CTGGAG 1 cut(s) 489
Bpu10I CCTNAGC 1 cut(s) 356
Bpu14I TTCGAA 1 cut(s) 367
Bsa29I ATCGAT 1 cut(s) 90
BsaAI YACGTR 1 cut(s) 768
BsaJI CCNNGG 2 cut(s) 42, 735
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 43
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 1 cut(s) 472
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 44
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 42, 735
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 13, 43
BseMI GCAATG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 472
BseXI GCAGC 2 cut(s) 590, 740
BseYI CCCAGC 1 cut(s) 513
BsgI GTGCAG 1 cut(s) 747
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 740
BshVI ATCGAT 1 cut(s) 90
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 202
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 1 cut(s) 456
BslI CCNNNNNNNGG 2 cut(s) 13, 43
BsmAI GTCTC 1 cut(s) 179
BsnI GGCC 1 cut(s) 740
BsoBI CYCGRG 1 cut(s) 189
Bsp119I TTCGAA 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 202
Bsp143I GATC 1 cut(s) 433
BspANI GGCC 1 cut(s) 740
BspDI ATCGAT 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 472
BspT104I TTCGAA 1 cut(s) 367
BsrDI GCAATG 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 455
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 2 cut(s) 42, 735
BssMI GATC 1 cut(s) 433
BssNAI GTATAC 1 cut(s) 595
Bst1107I GTATAC 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 16, 64
BstBAI YACGTR 1 cut(s) 768
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 3 cut(s) 133, 453, 526
BstDEI CTNAG 3 cut(s) 356, 395, 722
BstF5I GGATG 1 cut(s) 408
BstHHI GCGC 2 cut(s) 73, 547
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 1 cut(s) 433
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 422, 584, 737
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 42
BstSFI CTRYAG 1 cut(s) 654
BstSNI TACGTA 1 cut(s) 768
BstV1I GCAGC 2 cut(s) 590, 740
BstZ17I GTATAC 1 cut(s) 595
Bsu15I ATCGAT 1 cut(s) 90
BsuI GTATCC 1 cut(s) 705
BsuRI GGCC 1 cut(s) 740
BsuTUI ATCGAT 1 cut(s) 90
BtsCI GGATG 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 547
BtsIMutI CAGTG 1 cut(s) 547
Cac8I GCNNGC 3 cut(s) 133, 453, 526
CfoI GCGC 2 cut(s) 73, 547
Cfr10I RCCGGY 1 cut(s) 455
ClaI ATCGAT 1 cut(s) 90
Csp6I GTAC 2 cut(s) 4, 220
CviAII CATG 4 cut(s) 235, 426, 461, 536
CviQI GTAC 2 cut(s) 4, 220
DdeI CTNAG 3 cut(s) 356, 395, 722
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 738
Eco105I TACGTA 1 cut(s) 768
Eco88I CYCGRG 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 42
FaeI CATG 4 cut(s) 238, 429, 464, 539
FalI AAGNNNNNCTT 2 cut(s) 676, 708
FatI CATG 4 cut(s) 234, 425, 460, 535
FblI GTMKAC 2 cut(s) 231, 594
Fnu4HI GCNGC 2 cut(s) 579, 729
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 2 cut(s) 579, 729
FspAI RTGCGCAY 1 cut(s) 546
FspBI CTAG 2 cut(s) 227, 405
FspI TGCGCA 1 cut(s) 546
GlaI GCGC 2 cut(s) 72, 546
GluI GCNGC 2 cut(s) 579, 729
GsaI CCCAGC 1 cut(s) 517
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 1 cut(s) 740
HapII CCGG 1 cut(s) 456
HhaI GCGC 2 cut(s) 73, 547
Hin1II CATG 4 cut(s) 238, 429, 464, 539
Hin6I GCGC 2 cut(s) 71, 545
HinP1I GCGC 2 cut(s) 71, 545
HincII GTYRAC 2 cut(s) 19, 232
HindII GTYRAC 2 cut(s) 19, 232
HinfI GANTC 5 cut(s) 173, 187, 419, 650, 665
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 1 cut(s) 456
Hpy166II GTNNAC 3 cut(s) 19, 232, 595
Hpy188III TCNNGA 2 cut(s) 162, 662
Hpy8I GTNNAC 3 cut(s) 19, 232, 595
Hpy99I CGWCG 1 cut(s) 569
HpyAV CCTTC 2 cut(s) 353, 484
HpyCH4III ACNGT 2 cut(s) 16, 64
HpyCH4IV ACGT 1 cut(s) 767
HpyCH4V TGCA 6 cut(s) 131, 182, 425, 578, 611, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 422, 584, 737
HpyF3I CTNAG 3 cut(s) 356, 395, 722
HpySE526I ACGT 1 cut(s) 767
Hsp92II CATG 4 cut(s) 238, 429, 464, 539
HspAI GCGC 2 cut(s) 71, 545
KspAI GTTAAC 1 cut(s) 19
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 2 cut(s) 470, 736
Lsp1109I GCAGC 2 cut(s) 590, 740
LweI GCATC 3 cut(s) 96, 417, 631
MaeI CTAG 2 cut(s) 227, 405
MaeII ACGT 1 cut(s) 767
MaeIII GTNAC 2 cut(s) 64, 463
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 1 cut(s) 239
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 6 cut(s) 31, 122, 243, 267, 288, 622
MlyI GAGTC 1 cut(s) 181
MnlI CCTC 6 cut(s) 17, 169, 294, 533, 745, 757
MseI TTAA 3 cut(s) 18, 291, 383
MslI CAYNNNNRTG 1 cut(s) 540
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 44
MwoI GCNNNNNNNGC 3 cut(s) 422, 584, 737
NdeII GATC 1 cut(s) 433
NlaIII CATG 4 cut(s) 238, 429, 464, 539
NlaIV GGNNCC 1 cut(s) 472
NmeAIII GCCGAG 1 cut(s) 716
NsbI TGCGCA 1 cut(s) 546
NspI RCATGY 1 cut(s) 238
NspV TTCGAA 1 cut(s) 367
PfeI GAWTC 4 cut(s) 173, 419, 650, 665
PkrI GCNGC 2 cut(s) 580, 730
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Ppu21I YACGTR 1 cut(s) 768
PsiI TTATAA 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 42
PspFI CCCAGC 1 cut(s) 513
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 472
RsaI GTAC 2 cut(s) 5, 221
RsaNI GTAC 2 cut(s) 4, 220
RseI CAYNNNNRTG 1 cut(s) 540
SalI GTCGAC 1 cut(s) 230
SaqAI TTAA 3 cut(s) 18, 291, 383
SatI GCNGC 2 cut(s) 579, 729
Sau3AI GATC 1 cut(s) 433
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 44
SduI GDGCHC 1 cut(s) 202
SfaNI GCATC 3 cut(s) 96, 417, 631
SfcI CTRYAG 1 cut(s) 654
SfuI TTCGAA 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 540
SnaBI TACGTA 1 cut(s) 768
SpeI ACTAGT 1 cut(s) 226
Sse9I AATT 6 cut(s) 31, 122, 243, 267, 288, 622
SspI AATATT 1 cut(s) 209
SspMI CTAG 2 cut(s) 227, 405
StyD4I CCNGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 16, 64
TaiI ACGT 1 cut(s) 770
TaqI TCGA 6 cut(s) 90, 163, 231, 299, 367, 799
TasI AATT 6 cut(s) 31, 122, 243, 267, 288, 622
TfiI GAWTC 4 cut(s) 173, 419, 650, 665
Tru1I TTAA 3 cut(s) 18, 291, 383
Tru9I TTAA 3 cut(s) 18, 291, 383
TscAI CASTG 1 cut(s) 554
TseI GCWGC 2 cut(s) 578, 728
TspDTI ATGAA 2 cut(s) 98, 635
TspGWI ACGGA 2 cut(s) 116, 217
TspRI CASTG 1 cut(s) 554
XapI RAATTY 1 cut(s) 622
XceI RCATGY 1 cut(s) 238
XmiI GTMKAC 2 cut(s) 231, 594
XspI CTAG 2 cut(s) 227, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.