Rorug05G0300400

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000755
Physical Location & Seq
Reverse (-)
34918184 .. 34918491
308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0300400.1

Sequence Viewer

Length: 210 bp
ATGCAGCTGGGCGGCCTTGGCGAGATCGATCTTCTCGGGGTCGACGGCGAGGTCGCGGAGCTTCTTGGAGAGGCTGAGGGCGCGGAGGGTGCGGTGGAGGAGCTCGGAGGTGGCGTGGAGGTTGGAGAGCTGGAGAGTGAGGGCGGTGATGGAGCGGAGGGGGTCGGAGAGAGCTGTCAATTTCTAATTGTTCTGGGTCCGTTTTGTTAA

Protein Analysis

69

Amino Acids

6.76

Weight (kDa)

4.05

Isoelectric Point (pI)

22.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 50
AccBSI CCGCTC 1 cut(s) 155
AccI GTMKAC 1 cut(s) 42
AccII CGCG 2 cut(s) 56, 83
AciI CCGC 6 cut(s) 12, 56, 83, 92, 144, 155
AluBI AGCT 5 cut(s) 7, 61, 103, 130, 174
AluI AGCT 5 cut(s) 7, 61, 103, 130, 174
Alw21I GWGCWC 1 cut(s) 105
Ama87I CYCGRG 1 cut(s) 35
AoxI GGCC 1 cut(s) 13
ApeKI GCWGC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 35
AvaII GGWCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 143
BceAI ACGGC 1 cut(s) 61
BisI GCNGC 2 cut(s) 5, 13
BlsI GCNGC 2 cut(s) 6, 14
Bme18I GGWCC 1 cut(s) 197
BmeT110I CYCGRG 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 75
Bsa29I ATCGAT 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 16
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 1 cut(s) 16
BseYI CCCAGC 1 cut(s) 7
Bsh1236I CGCG 2 cut(s) 56, 83
BshFI GGCC 1 cut(s) 15
BshVI ATCGAT 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 35
BsnI GGCC 1 cut(s) 15
BsoBI CYCGRG 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 2 cut(s) 24, 28
BspACI CCGC 6 cut(s) 12, 56, 83, 92, 144, 155
BspANI GGCC 1 cut(s) 15
BspCNI CTCAG 1 cut(s) 67
BspDI ATCGAT 1 cut(s) 27
BspFNI CGCG 2 cut(s) 56, 83
BspLI GGNNCC 1 cut(s) 198
BsrBI CCGCTC 1 cut(s) 155
BssECI CCNNGG 1 cut(s) 16
BssMI GATC 2 cut(s) 24, 28
BssT1I CCWWGG 1 cut(s) 16
BstDEI CTNAG 1 cut(s) 75
BstFNI CGCG 2 cut(s) 56, 83
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 2 cut(s) 27, 31
BstMBI GATC 2 cut(s) 24, 28
BstMWI GCNNNNNNNGC 3 cut(s) 18, 80, 89
BstUI CGCG 2 cut(s) 56, 83
BstV1I GCAGC 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 27
BsuRI GGCC 1 cut(s) 15
BsuTUI ATCGAT 1 cut(s) 27
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 197
ClaI ATCGAT 1 cut(s) 27
CviJI RGCY 7 cut(s) 7, 15, 61, 74, 103, 130, 174
CviKI_1 RGCY 7 cut(s) 7, 15, 61, 74, 103, 130, 174
DdeI CTNAG 1 cut(s) 75
DpnI GATC 2 cut(s) 26, 30
DpnII GATC 2 cut(s) 24, 28
DrdI GACNNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 50
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 16
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 5, 13
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 5, 13
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 5, 13
GsaI CCCAGC 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 15
HhaI GCGC 1 cut(s) 83
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 107, 167
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 1 cut(s) 47
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 80, 89
HpyF3I CTNAG 1 cut(s) 75
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 24, 28
LmnI GCTCC 3 cut(s) 58, 100, 152
LpnPI CCDG 2 cut(s) 116, 179
Lsp1109I GCAGC 1 cut(s) 16
MalI GATC 2 cut(s) 26, 30
MbiI CCGCTC 1 cut(s) 155
MboI GATC 2 cut(s) 24, 28
MboII GAAGA 1 cut(s) 23
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 2 cut(s) 179, 186
MmeI TCCRAC 2 cut(s) 103, 145
MnlI CCTC 9 cut(s) 43, 64, 70, 79, 91, 101, 112, 133, 151
MseI TTAA 1 cut(s) 208
MspA1I CMGCKG 1 cut(s) 7
MvnI CGCG 2 cut(s) 56, 83
MwoI GCNNNNNNNGC 3 cut(s) 18, 80, 89
NdeII GATC 2 cut(s) 24, 28
NlaIV GGNNCC 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PkrI GCNGC 2 cut(s) 6, 14
Psp124BI GAGCTC 1 cut(s) 105
PspFI CCCAGC 1 cut(s) 7
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 7
SacI GAGCTC 1 cut(s) 105
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 208
SatI GCNGC 2 cut(s) 5, 13
Sau3AI GATC 2 cut(s) 24, 28
Sau96I GGNCC 1 cut(s) 197
SduI GDGCHC 1 cut(s) 105
SetI ASST 8 cut(s) 9, 54, 63, 105, 112, 123, 132, 176
SinI GGWCC 1 cut(s) 197
Sse9I AATT 2 cut(s) 179, 186
SsiI CCGC 6 cut(s) 12, 56, 83, 92, 144, 155
SstI GAGCTC 1 cut(s) 105
StyI CCWWGG 1 cut(s) 16
TaqI TCGA 2 cut(s) 27, 42
TasI AATT 2 cut(s) 179, 186
TauI GCSGC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 208
Tru9I TTAA 1 cut(s) 208
TseI GCWGC 1 cut(s) 4
TspGWI ACGGA 1 cut(s) 189
VpaK11BI GGWCC 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.