Rroxscaffold_4G00279460

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000755
Physical Location & Seq
Reverse (-)
2257712 .. 2260309
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279460.1

Sequence Viewer

Length: 1611 bp
ATGGAGGCTCAAATTTCAAGGACACAGAGCTGTGATTTAGAAGAGAATGCTGAAGAAAAAACTCAAAAGCCTAATGGTACTACTCAGTCAGGTGCAAGTTCAGCATCAAACTCCTTGGTTGTAAGTAAGTTCGAAGGGCTAGTGAGTTTTGGCAGAACAATGAAAACTTATTATGGAGTCTTGTCCATAGGCTTAAGTTCCAAGAATAGGAAACTTGACTGCCAACATGAATTAGTCCAGCAAAGGTGGAATAAGATACTTCTTTTTGTGTGTGTTATTGCTCTATTACTGGATCCTTTGTTTTGTTATCTCCTTGTGGTCAATGATGAGAAGAAGTGCCTTGAATTAGACAGAAGACTAGAGGTCATAGCCATTGCTTTGCGATTTGTGGTGGATTTCTTTTATCTATTATATATCATTTATCGATTTCGTGCTAGTGTTGTTACTCTTTCCTCTCCATCATCAAGAGAAGGTGAATTGGTTCCAGATACTCGGATAATAGCAAGGAAATATTTGTTCTTCTACTTCCTATTCGACATACTTGTGATTCTTCCACTTCCACAGGTGGTAGTTTTTCATGTCATTCCAAGATTGAATAACCCAAGGGTTTTCCATGCAACAGAGTCATTGTACTTTATACTTATCTTTCAATATGTGCTAAGGGTTCTTCGAATCTGTTCCTTGTTAAACGAAGCTACCAGGTCTTCTGGCATCCTAGCCGAAACTGCATGGGCTAAAGCTGCCTTTAATCTCTTTCTTTACATGCTTGCTAGTCATGTTGCATGTTGGAGCCTTTTGGTACTTCTTTTCGATAGAAAGGAAAGCATCTTGCTGGCGAAAATCCTCTCCCTTGGTCAAGGCCTCAAAACAAGTACCTATGTATGGGAAATCTACTTCTCAGTTTTTGTTTCGCTTTCTGGCTTGATACTTTTTGCATTTCTTATTGGAAATATGCAGACATATTTACAATCGAAAACTGCAAGGTTGGAGGATATGAGATTGAAAGGGCAAGAGATAGAGTTGTGGATGGCTTTCCATTCCCTTCCTCGGGAGCTCAAGAATCAAATAAGGCGATACCAGAAATACAAATGGCAAGAAACTAGAGGTGTTGACGTAGAGAGTTTCCTTCACAATCTTCCCAAAGACATCAGAAGAGAAACAAAGCAATATCTTTGCTCAGGTCCACTCAGAAAAGTTACAATGCTTCAAAACATGGATCAACGATTGTTTGACGCAATCTGTGATCATTTGAAGCCAGTGCTTTGCATAGACCACAACTTCATAATGCGAAAGGGAGACCCTCTCGATGAGATGTTGTTCATCACTCGTGGCCAAGTATTGACTTACACCACAGAAGACAGTGAAAGCGCTGTTTGTTTTGAAAAAGGTGACTTCTATGGCCAAGAACTTCTTGACTGGGTGCTCAAGAATCCCAATCCCAGCCTATCCAACCTTCCCTTCTCAACCAAAACAGTCCAAGCACATACCAACGTTGAAGTGTTTGCTCTTAGGGCCAATGACTTGAAGAATGTAGTCTCTAAGTTCTGGTGGCTTTTCCCCAGGTCTGGTTTGGAGCAATCGCTCAAACCAAGGGCAGCTCAGGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

536

Amino Acids

61.9

Weight (kDa)

8.94

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 1491
AclWI GGATC 3 cut(s) 287, 300, 1224
AcoI YGGCCR 2 cut(s) 1330, 1399
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 4 cut(s) 79, 632, 801, 874
AfeI AGCGCT 1 cut(s) 1369
AfiI CCNNNNNNNGG 5 cut(s) 207, 882, 1047, 1048, 1565
AflII CTTAAG 1 cut(s) 193
AhdI GACNNNNNGTC 1 cut(s) 362
AjnI CCWGG 2 cut(s) 698, 1559
AjuI GAANNNNNNNTTGG 2 cut(s) 1442, 1474
AluBI AGCT 5 cut(s) 30, 695, 740, 1054, 1598
AluI AGCT 5 cut(s) 30, 695, 740, 1054, 1598
Alw21I GWGCWC 2 cut(s) 1056, 1425
Alw26I GTCTC 2 cut(s) 1290, 1540
AlwI GGATC 3 cut(s) 287, 300, 1224
Ama87I CYCGRG 1 cut(s) 1047
Aor51HI AGCGCT 1 cut(s) 1369
AoxI GGCC 4 cut(s) 859, 1330, 1399, 1512
ApeKI GCWGC 2 cut(s) 740, 1595
ApoI RAATTY 1 cut(s) 12
Asp700I GAANNNNTTC 2 cut(s) 480, 676
AspLEI GCGC 1 cut(s) 1370
AspS9I GGNCC 2 cut(s) 1181, 1512
AsuHPI GGTGA 2 cut(s) 485, 1400
AsuII TTCGAA 2 cut(s) 132, 670
AvaI CYCGRG 1 cut(s) 1047
AvaII GGWCC 1 cut(s) 1181
BalI TGGCCA 2 cut(s) 1332, 1401
BamHI GGATCC 1 cut(s) 292
BanII GRGCYC 1 cut(s) 1056
BauI CACGAG 1 cut(s) 1326
BbsI GAAGAC 3 cut(s) 361, 696, 1362
Bbv12I GWGCWC 2 cut(s) 1056, 1425
BbvI GCAGC 2 cut(s) 727, 1607
BccI CCATC 2 cut(s) 466, 1021
BciT130I CCWGG 2 cut(s) 700, 1561
BclI TGATCA 1 cut(s) 1243
BcoDI GTCTC 2 cut(s) 1290, 1540
BfaI CTAG 6 cut(s) 140, 359, 435, 716, 771, 1101
BfoI RGCGCY 1 cut(s) 1371
BfrI CTTAAG 1 cut(s) 193
BisI GCNGC 2 cut(s) 741, 1596
BlsI GCNGC 2 cut(s) 742, 1597
Bme1390I CCNGG 2 cut(s) 700, 1561
Bme18I GGWCC 1 cut(s) 1181
BmeRI GACNNNNNGTC 1 cut(s) 362
BmeT110I CYCGRG 1 cut(s) 1047
BmgT120I GGNCC 2 cut(s) 1181, 1512
BmiI GGNNCC 3 cut(s) 294, 483, 791
BmrFI CCNGG 2 cut(s) 700, 1561
BmrI ACTGGG 1 cut(s) 1426
BmsI GCATC 3 cut(s) 113, 720, 834
BmuI ACTGGG 1 cut(s) 1426
BpiI GAAGAC 3 cut(s) 361, 696, 1362
BplI GAGNNNNNCTC 2 cut(s) 1287, 1319
Bpu10I CCTNAGC 3 cut(s) 659, 1177, 1599
Bpu14I TTCGAA 2 cut(s) 132, 670
BpuEI CTTGAG 2 cut(s) 1040, 1409
Bsa29I ATCGAT 1 cut(s) 424
BsaI GGTCTC 1 cut(s) 1290
BsaJI CCNNGG 6 cut(s) 114, 602, 850, 1046, 1559, 1589
Bsc4I CCNNNNNNNGG 5 cut(s) 207, 882, 1047, 1048, 1565
Bse1I ACTGG 3 cut(s) 294, 1256, 1421
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 2 cut(s) 700, 1561
BseCI ATCGAT 1 cut(s) 424
BseDI CCNNGG 6 cut(s) 114, 602, 850, 1046, 1559, 1589
BseGI GGATG 2 cut(s) 711, 1032
BseLI CCNNNNNNNGG 5 cut(s) 207, 882, 1047, 1048, 1565
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 4 cut(s) 98, 912, 1191, 1201
BseNI ACTGG 3 cut(s) 294, 1256, 1421
BseXI GCAGC 2 cut(s) 727, 1607
BseYI CCCAGC 1 cut(s) 1439
BshFI GGCC 4 cut(s) 861, 1332, 1401, 1514
BshVI ATCGAT 1 cut(s) 424
BsiHKAI GWGCWC 2 cut(s) 1056, 1425
BsiHKCI CYCGRG 1 cut(s) 1047
BslI CCNNNNNNNGG 5 cut(s) 207, 882, 1047, 1048, 1565
BsmAI GTCTC 2 cut(s) 1290, 1540
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 4 cut(s) 861, 1332, 1401, 1514
Bso31I GGTCTC 1 cut(s) 1290
BsoBI CYCGRG 1 cut(s) 1047
Bsp119I TTCGAA 2 cut(s) 132, 670
Bsp1286I GDGCHC 2 cut(s) 1056, 1425
Bsp143I GATC 3 cut(s) 292, 1216, 1243
BspANI GGCC 4 cut(s) 861, 1332, 1401, 1514
BspCNI CTCAG 4 cut(s) 97, 911, 1190, 1200
BspDI ATCGAT 1 cut(s) 424
BspLI GGNNCC 3 cut(s) 294, 483, 791
BspPI GGATC 3 cut(s) 287, 300, 1224
BspT104I TTCGAA 2 cut(s) 132, 670
BspTI CTTAAG 1 cut(s) 193
BspTNI GGTCTC 1 cut(s) 1290
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 3 cut(s) 294, 1256, 1421
BssECI CCNNGG 6 cut(s) 114, 602, 850, 1046, 1559, 1589
BssMI GATC 3 cut(s) 292, 1216, 1243
BssSI CACGAG 1 cut(s) 1326
BssT1I CCWWGG 4 cut(s) 114, 602, 850, 1589
Bst2BI CACGAG 1 cut(s) 1326
Bst2UI CCWGG 2 cut(s) 700, 1561
Bst4CI ACNGT 2 cut(s) 1361, 1474
Bst6I CTCTTC 2 cut(s) 36, 1147
BstAFI CTTAAG 1 cut(s) 193
BstBI TTCGAA 2 cut(s) 132, 670
BstC8I GCNNGC 2 cut(s) 768, 834
BstDEI CTNAG 8 cut(s) 84, 659, 898, 1177, 1187, 1508, 1539, 1599
BstF5I GGATG 2 cut(s) 711, 1032
BstH2I RGCGCY 1 cut(s) 1371
BstHHI GCGC 1 cut(s) 1370
BstKTI GATC 3 cut(s) 295, 1219, 1246
BstMAI GTCTC 2 cut(s) 1290, 1540
BstMBI GATC 3 cut(s) 292, 1216, 1243
BstMWI GCNNNNNNNGC 5 cut(s) 101, 725, 740, 1511, 1601
BstNI CCWGG 2 cut(s) 700, 1561
BstNSI RCATGY 2 cut(s) 766, 786
BstSCI CCNGG 2 cut(s) 698, 1559
BstV1I GCAGC 2 cut(s) 727, 1607
BstV2I GAAGAC 3 cut(s) 361, 696, 1362
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
Bsu15I ATCGAT 1 cut(s) 424
BsuRI GGCC 4 cut(s) 861, 1332, 1401, 1514
BsuTUI ATCGAT 1 cut(s) 424
BtsCI GGATG 2 cut(s) 711, 1032
BtsIMutI CAGTG 2 cut(s) 1263, 1366
Cac8I GCNNGC 2 cut(s) 768, 834
CfoI GCGC 1 cut(s) 1370
Cfr13I GGNCC 2 cut(s) 1181, 1512
ClaI ATCGAT 1 cut(s) 424
CseI GACGC 1 cut(s) 1241
CsiI ACCWGGT 1 cut(s) 698
Csp6I GTAC 4 cut(s) 78, 631, 800, 873
CviAII CATG 8 cut(s) 227, 578, 614, 729, 763, 776, 783, 1213
CviQI GTAC 4 cut(s) 78, 631, 800, 873
DdeI CTNAG 8 cut(s) 84, 659, 898, 1177, 1187, 1508, 1539, 1599
DpnI GATC 3 cut(s) 294, 1218, 1245
DpnII GATC 3 cut(s) 292, 1216, 1243
DriI GACNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 2 cut(s) 1330, 1399
Eam1104I CTCTTC 2 cut(s) 36, 1147
Eam1105I GACNNNNNGTC 1 cut(s) 362
EarI CTCTTC 2 cut(s) 36, 1147
Ecl136II GAGCTC 1 cut(s) 1054
Eco130I CCWWGG 4 cut(s) 114, 602, 850, 1589
Eco147I AGGCCT 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 1056
Eco31I GGTCTC 1 cut(s) 1290
Eco47I GGWCC 1 cut(s) 1181
Eco47III AGCGCT 1 cut(s) 1369
Eco53kI GAGCTC 1 cut(s) 1054
Eco57I CTGAAG 1 cut(s) 72
Eco88I CYCGRG 1 cut(s) 1047
EcoICRI GAGCTC 1 cut(s) 1054
EcoRII CCWGG 2 cut(s) 698, 1559
EcoT14I CCWWGG 4 cut(s) 114, 602, 850, 1589
EcoT38I GRGCYC 1 cut(s) 1056
ErhI CCWWGG 4 cut(s) 114, 602, 850, 1589
FaeI CATG 8 cut(s) 230, 581, 617, 732, 766, 779, 786, 1216
FalI AAGNNNNNCTT 2 cut(s) 1395, 1427
FatI CATG 8 cut(s) 226, 577, 613, 728, 762, 775, 782, 1212
FbaI TGATCA 1 cut(s) 1243
Fnu4HI GCNGC 2 cut(s) 741, 1596
FokI GGATG 2 cut(s) 698, 1039
FriOI GRGCYC 1 cut(s) 1056
Fsp4HI GCNGC 2 cut(s) 741, 1596
FspBI CTAG 6 cut(s) 140, 359, 435, 716, 771, 1101
GlaI GCGC 1 cut(s) 1369
GluI GCNGC 2 cut(s) 741, 1596
GsaI CCCAGC 1 cut(s) 1443
HaeII RGCGCY 1 cut(s) 1371
HaeIII GGCC 4 cut(s) 861, 1332, 1401, 1514
HgaI GACGC 1 cut(s) 1241
HhaI GCGC 1 cut(s) 1370
Hin1II CATG 8 cut(s) 230, 581, 617, 732, 766, 779, 786, 1216
Hin6I GCGC 1 cut(s) 1368
HinP1I GCGC 1 cut(s) 1368
HincII GTYRAC 1 cut(s) 1111
HindII GTYRAC 1 cut(s) 1111
HinfI GANTC 6 cut(s) 177, 547, 623, 672, 1060, 1429
HphI GGTGA 2 cut(s) 485, 1400
Hpy166II GTNNAC 2 cut(s) 1111, 1184
Hpy188I TCNGA 3 cut(s) 495, 1151, 1190
Hpy188III TCNNGA 7 cut(s) 465, 485, 1049, 1057, 1304, 1412, 1426
Hpy8I GTNNAC 2 cut(s) 1111, 1184
HpyAV CCTTC 6 cut(s) 128, 464, 1052, 1136, 1463, 1468
HpyCH4III ACNGT 2 cut(s) 1361, 1474
HpyCH4IV ACGT 2 cut(s) 1113, 1491
HpyCH4V TGCA 8 cut(s) 95, 617, 728, 782, 935, 955, 980, 1266
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 725, 740, 1511, 1601
HpyF3I CTNAG 8 cut(s) 84, 659, 898, 1177, 1187, 1508, 1539, 1599
HpySE526I ACGT 2 cut(s) 1113, 1491
Hsp92II CATG 8 cut(s) 230, 581, 617, 732, 766, 779, 786, 1216
HspAI GCGC 1 cut(s) 1368
Ksp22I TGATCA 1 cut(s) 1243
Kzo9I GATC 3 cut(s) 292, 1216, 1243
LmnI GCTCC 3 cut(s) 789, 1051, 1573
Lsp1109I GCAGC 2 cut(s) 727, 1607
LweI GCATC 3 cut(s) 113, 720, 834
MabI ACCWGGT 1 cut(s) 698
MaeI CTAG 6 cut(s) 140, 359, 435, 716, 771, 1101
MaeII ACGT 2 cut(s) 1113, 1491
MaeIII GTNAC 3 cut(s) 442, 1195, 1388
MalI GATC 3 cut(s) 294, 1218, 1245
MboI GATC 3 cut(s) 292, 1216, 1243
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 2 cut(s) 1056, 1425
MlsI TGGCCA 2 cut(s) 1332, 1401
MluCI AATT 4 cut(s) 12, 230, 344, 476
MluNI TGGCCA 2 cut(s) 1332, 1401
MlyI GAGTC 2 cut(s) 186, 632
MmeI TCCRAC 3 cut(s) 767, 966, 1473
MnlI CCTC 8 cut(s) 355, 463, 854, 872, 982, 1056, 1097, 1311
Mox20I TGGCCA 2 cut(s) 1332, 1401
MroXI GAANNNNTTC 2 cut(s) 480, 676
MscI TGGCCA 2 cut(s) 1332, 1401
MseI TTAA 3 cut(s) 194, 686, 747
MslI CAYNNNNRTG 1 cut(s) 542
Msp20I TGGCCA 2 cut(s) 1332, 1401
MspCI CTTAAG 1 cut(s) 193
MspR9I CCNGG 2 cut(s) 700, 1561
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 2 cut(s) 700, 1561
MwoI GCNNNNNNNGC 5 cut(s) 101, 725, 740, 1511, 1601
NdeII GATC 3 cut(s) 292, 1216, 1243
NlaIII CATG 8 cut(s) 230, 581, 617, 732, 766, 779, 786, 1216
NlaIV GGNNCC 3 cut(s) 294, 483, 791
NmuCI GTSAC 1 cut(s) 1388
NspI RCATGY 2 cut(s) 766, 786
NspV TTCGAA 2 cut(s) 132, 670
PceI AGGCCT 1 cut(s) 861
PctI GAATGC 1 cut(s) 52
PdmI GAANNNNTTC 2 cut(s) 480, 676
PfeI GAWTC 4 cut(s) 547, 672, 1060, 1429
PkrI GCNGC 2 cut(s) 742, 1597
PleI GAGTC 2 cut(s) 185, 631
PpsI GAGTC 2 cut(s) 185, 631
Psp124BI GAGCTC 1 cut(s) 1056
Psp1406I AACGTT 1 cut(s) 1491
Psp6I CCWGG 2 cut(s) 698, 1559
PspFI CCCAGC 1 cut(s) 1439
PspGI CCWGG 2 cut(s) 698, 1559
PspN4I GGNNCC 3 cut(s) 294, 483, 791
PspPI GGNCC 2 cut(s) 1181, 1512
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 4 cut(s) 79, 632, 801, 874
RsaNI GTAC 4 cut(s) 78, 631, 800, 873
RseI CAYNNNNRTG 1 cut(s) 542
SacI GAGCTC 1 cut(s) 1056
SaqAI TTAA 3 cut(s) 194, 686, 747
SatI GCNGC 2 cut(s) 741, 1596
Sau3AI GATC 3 cut(s) 292, 1216, 1243
Sau96I GGNCC 2 cut(s) 1181, 1512
SchI GAGTC 2 cut(s) 186, 632
ScrFI CCNGG 2 cut(s) 700, 1561
SduI GDGCHC 2 cut(s) 1056, 1425
SexAI ACCWGGT 1 cut(s) 698
SfaNI GCATC 3 cut(s) 113, 720, 834
SfuI TTCGAA 2 cut(s) 132, 670
SinI GGWCC 1 cut(s) 1181
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 3 cut(s) 193, 1055, 1424
SmoI CTYRAG 3 cut(s) 193, 1055, 1424
Sse9I AATT 4 cut(s) 12, 230, 344, 476
SseBI AGGCCT 1 cut(s) 861
SspI AATATT 1 cut(s) 512
SspMI CTAG 6 cut(s) 140, 359, 435, 716, 771, 1101
SstI GAGCTC 1 cut(s) 1056
StuI AGGCCT 1 cut(s) 861
StyD4I CCNGG 2 cut(s) 698, 1559
StyI CCWWGG 4 cut(s) 114, 602, 850, 1589
TaaI ACNGT 2 cut(s) 1361, 1474
TaiI ACGT 2 cut(s) 1116, 1494
TaqI TCGA 7 cut(s) 132, 424, 534, 670, 810, 971, 1305
TasI AATT 4 cut(s) 12, 230, 344, 476
TatI WGTACW 1 cut(s) 630
TfiI GAWTC 4 cut(s) 547, 672, 1060, 1429
Tru1I TTAA 3 cut(s) 194, 686, 747
Tru9I TTAA 3 cut(s) 194, 686, 747
TscAI CASTG 2 cut(s) 1263, 1366
TseFI GTSAC 1 cut(s) 1388
TseI GCWGC 2 cut(s) 740, 1595
Tsp45I GTSAC 1 cut(s) 1388
TspDTI ATGAA 5 cut(s) 176, 243, 566, 1270, 1309
TspRI CASTG 2 cut(s) 1263, 1366
Vha464I CTTAAG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 1181
XapI RAATTY 1 cut(s) 12
XceI RCATGY 2 cut(s) 766, 786
XcmI CCANNNNNNNNNTGG 1 cut(s) 1567
XmnI GAANNNNTTC 2 cut(s) 480, 676
XspI CTAG 6 cut(s) 140, 359, 435, 716, 771, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.