Rorug01G0419900

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000755
Physical Location & Seq
Reverse (-)
52161414 .. 52163195
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0419900.1

Sequence Viewer

Length: 1782 bp
ATGGGAGAGGCTGTTCTTGGGGCATTCCTCCCGGTGCTGCTTGAGAAGTTGGCGAATCGAGAGGTCATTGAGTACTTTGGACGCGTTAAGGGGGTCGACAAAAAGGTGCTGGAGAAATGGACGAGAACGTTGACTGCAATTGAAGCGGTGCTGAGTGATGCGGAGGAGAAGCAACTAACACAAAGAGCAGTGAAACTATGGCTAGATGATCTCAGAGATTTGGCTTACGATGTTCAAGACATATTGGACACATTTGATACTGAAATGCTGAAGCGAAGGATAGAAAGACAACAAGGAAGCACAAGTAATTCATGGATCAGCTACCTCTCTAAACCTAAGTTCAATTTTAGTGTGAACTCAGAAATCAAGAAGATAAGTGATCGACTAGAAGAGATAACTACTCGAGAAAAGCATTTTGGTTTGAAGAAACTTGGAGTGTCTAAAAAGCCATGGAAAATGCCACCCACTACTTCTCAACTAGATGGACCTGTGATCGGAAGGAATGAAGCAAAAGAAAAAATTCTTGATGATCTGCTCTCAAATGGCGAACATAGTACCAGCAATTTTCAGAATTTTCATGTGGTTTCCATTGTTGGTACGGGTGGAGTAGGAAAGACAACACTGGCTAAAGATGTATTCAACGACGCTGCAACTGAACAATTTTGCCCAAAGGGATGGGTGTGTGTGTCCGATGACTTCGACCTTCTAAGGTTGACAACTGCAGTTCTTCAATCTGTCACGTCAGGTGATGTGAAGGAATCCAAAGAGCTCAATTCAGTTCAGGAGAAGCTGAGTAAGGAATTGGCTGGCAAAAAGTTTTTAATTGTTCTAGATGATGTCTGGAATACATGCAACTACGATCAATGGACAACATTGCAGTCCGCCTTTCGTGTTGGAGCAGTAGGAAGCAAGATCATTGTGACGACGCGTGATGCGAATGTTGCAAAGATGATGGGAGACAGTAGCCCTTATAACTTGGAGTCGATGTCAAAAGATGATTGTTGGAAAATCTTTGTCCACCATGCACTCTTAAACAATACACCCCAAGATGTTGCGTTACTGAAGGAGAAAATTGTAGTAAAATGCAATGGATTGCCGTTGGCTGCAAAGACTCTTGGTGGTCTTTTACGTTGTAAAACAGTAGACGAACGGGAAGAAATATTAGACCACAAGATGTGGAATCTATCCAACCAGATGGGCATCCTTCCAGCATTACAGTTGAGCTATCATTATCTCCCCTCGAATTTGAAACGGTGCTTCACCTACTGTGCAATTCTTCCAAATGATTACGAATTTGGGGAGATGCAATTGATCCTTCTGTGGATGGCAGAGGGTTTGATTCCGCAGGAAGCCGAAGAGAATAAACAAATGGAAGATTTGGGCCGTCACTATTTTCGAGAGTTGGTGTCTAGGTCATTATTTCAAAAATCAAGCAAAGGAAACTCGCGATACATAATGCATGAGCTCATTACTGATTTGGCACGTTGGGCTGCAGGAAATTCATGTTCTAGATTGGAGGATATGCAGAATTATGACTCATCGCGACGTAGATTGGTTCGTCATTCGTCTCACATTCCTGGTATGTATGATGGGATTAAAAAGTTTGGGGTCTATTCTGAAGCTATATGTTTGCGGACATTCTTACCACTTTCACTTTCACTTTCAAATTATAATTATCTGGCTCAAAGGGTTACTTCTGATTTATTGCCCAAACTCCAAATACTTGCGACTGCTCTCTTTGAGTCGATATCAAATAACCGAGCTGCCAAATACAATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

593

Amino Acids

67.31

Weight (kDa)

7.91

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 98 2.8e-21 Rx N-terminal domain
NB-ARC PF00931 171 - 342 2.4e-35 NB-ARC domain
WHD_DRP PF23559 425 - 494 6.4e-23 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 972, 1671
AccI GTMKAC 2 cut(s) 96, 1143
AccII CGCG 4 cut(s) 84, 928, 1447, 1543
AciI CCGC 5 cut(s) 146, 161, 882, 1343, 1633
AclI AACGTT 1 cut(s) 128
AclWI GGATC 2 cut(s) 323, 1306
AcsI RAATTY 5 cut(s) 519, 571, 1243, 1292, 1498
AcuI CTGAAG 3 cut(s) 290, 1082, 1638
AfaI GTAC 3 cut(s) 74, 556, 598
AfiI CCNNNNNNNGG 1 cut(s) 494
AflIII ACRYGT 2 cut(s) 82, 926
AgsI TTSAA 9 cut(s) 143, 236, 343, 424, 640, 731, 1249, 1424, 1665
AjiI CACGTC 1 cut(s) 741
AjnI CCWGG 1 cut(s) 1576
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 7 cut(s) 321, 769, 790, 1224, 1465, 1622, 1763
AluI AGCT 7 cut(s) 321, 769, 790, 1224, 1465, 1622, 1763
Alw21I GWGCWC 2 cut(s) 771, 1467
Alw26I GTCTC 2 cut(s) 951, 1572
AlwI GGATC 2 cut(s) 323, 1306
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 1 cut(s) 1381
ApeKI GCWGC 5 cut(s) 37, 647, 1103, 1490, 1763
ApoI RAATTY 5 cut(s) 519, 571, 1243, 1292, 1498
Asp700I GAANNNNTTC 1 cut(s) 519
AspS9I GGNCC 2 cut(s) 485, 1381
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 2 cut(s) 758, 1252
AvaI CYCGRG 1 cut(s) 402
AvaII GGWCC 1 cut(s) 485
BanII GRGCYC 2 cut(s) 771, 1467
Bbv12I GWGCWC 2 cut(s) 771, 1467
BbvI GCAGC 5 cut(s) 24, 634, 1090, 1477, 1750
BccI CCATC 6 cut(s) 476, 669, 946, 1189, 1318, 1583
BceAI ACGGC 2 cut(s) 1081, 1368
BciT130I CCWGG 1 cut(s) 1578
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 951, 1572
BfaI CTAG 6 cut(s) 203, 386, 479, 830, 1410, 1509
BfmI CTRYAG 2 cut(s) 720, 1491
BisI GCNGC 5 cut(s) 38, 648, 1104, 1491, 1764
BlsI GCNGC 5 cut(s) 39, 649, 1105, 1492, 1765
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 2 cut(s) 32, 1578
Bme18I GGWCC 1 cut(s) 485
BmeT110I CYCGRG 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 485, 1381
BmrFI CCNGG 2 cut(s) 32, 1578
BmsI GCATC 4 cut(s) 148, 922, 1209, 1293
BpmI CTGGAG 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 1199
BsaJI CCNNGG 1 cut(s) 449
BsaXI ACNNNNNCTCC 3 cut(s) 27, 971, 1001
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse1I ACTGG 1 cut(s) 627
Bse3DI GCAATG 2 cut(s) 872, 1093
Bse8I GATNNNNATC 1 cut(s) 1199
BseBI CCWGG 1 cut(s) 1578
BseDI CCNNGG 1 cut(s) 449
BseGI GGATG 3 cut(s) 680, 1200, 1329
BseJI GATNNNNATC 1 cut(s) 1199
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMI GCAATG 2 cut(s) 872, 1093
BseMII CTCAG 4 cut(s) 143, 226, 372, 782
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 1 cut(s) 179
BseXI GCAGC 5 cut(s) 24, 634, 1090, 1477, 1750
Bsh1236I CGCG 4 cut(s) 84, 928, 1447, 1543
BshFI GGCC 1 cut(s) 1383
BsiHKAI GWGCWC 2 cut(s) 771, 1467
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 2 cut(s) 951, 1572
BsmBI CGTCTC 1 cut(s) 1572
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 1 cut(s) 1383
BsoBI CYCGRG 1 cut(s) 402
Bsp1286I GDGCHC 2 cut(s) 771, 1467
Bsp143I GATC 8 cut(s) 208, 315, 379, 492, 529, 859, 912, 1311
Bsp19I CCATGG 1 cut(s) 449
Bsp68I TCGCGA 2 cut(s) 1447, 1543
BspACI CCGC 5 cut(s) 146, 161, 882, 1343, 1633
BspANI GGCC 1 cut(s) 1383
BspCNI CTCAG 4 cut(s) 144, 225, 371, 783
BspFNI CGCG 4 cut(s) 84, 928, 1447, 1543
BspMAI CTGCAG 2 cut(s) 724, 1495
BspPI GGATC 2 cut(s) 323, 1306
BsrDI GCAATG 2 cut(s) 872, 1093
BsrI ACTGG 1 cut(s) 627
BssECI CCNNGG 1 cut(s) 449
BssMI GATC 8 cut(s) 208, 315, 379, 492, 529, 859, 912, 1311
BssT1I CCWWGG 1 cut(s) 449
Bst2UI CCWGG 1 cut(s) 1578
Bst4CI ACNGT 5 cut(s) 962, 1141, 1218, 1254, 1268
Bst6I CTCTTC 2 cut(s) 384, 1350
BstC8I GCNNGC 1 cut(s) 808
BstDEI CTNAG 6 cut(s) 152, 212, 336, 358, 707, 791
BstDSI CCRYGG 1 cut(s) 449
BstF5I GGATG 3 cut(s) 680, 1200, 1329
BstFNI CGCG 4 cut(s) 84, 928, 1447, 1543
BstKTI GATC 8 cut(s) 211, 318, 382, 495, 532, 862, 915, 1314
BstMAI GTCTC 2 cut(s) 951, 1572
BstMBI GATC 8 cut(s) 208, 315, 379, 492, 529, 859, 912, 1311
BstMWI GCNNNNNNNGC 3 cut(s) 143, 941, 1487
BstNI CCWGG 1 cut(s) 1578
BstNSI RCATGY 1 cut(s) 852
BstSCI CCNGG 2 cut(s) 30, 1576
BstSFI CTRYAG 2 cut(s) 720, 1491
BstUI CGCG 4 cut(s) 84, 928, 1447, 1543
BstV1I GCAGC 5 cut(s) 24, 634, 1090, 1477, 1750
BstXI CCANNNNNNTGG 2 cut(s) 675, 1195
BsuRI GGCC 1 cut(s) 1383
BtgI CCRYGG 1 cut(s) 449
BtgZI GCGATG 1 cut(s) 1524
BtrI CACGTC 1 cut(s) 741
BtsCI GGATG 3 cut(s) 680, 1200, 1329
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 620
BtuMI TCGCGA 2 cut(s) 1447, 1543
Cac8I GCNNGC 1 cut(s) 808
Cfr13I GGNCC 2 cut(s) 485, 1381
CseI GACGC 3 cut(s) 90, 653, 934
Csp6I GTAC 3 cut(s) 73, 555, 597
CviAII CATG 7 cut(s) 312, 450, 578, 849, 1022, 1460, 1503
CviQI GTAC 3 cut(s) 73, 555, 597
DdeI CTNAG 6 cut(s) 152, 212, 336, 358, 707, 791
DpnI GATC 8 cut(s) 210, 317, 381, 494, 531, 861, 914, 1313
DpnII GATC 8 cut(s) 208, 315, 379, 492, 529, 859, 912, 1311
Eam1104I CTCTTC 2 cut(s) 384, 1350
EarI CTCTTC 2 cut(s) 384, 1350
EciI GGCGGA 1 cut(s) 871
Ecl136II GAGCTC 2 cut(s) 769, 1465
Eco130I CCWWGG 1 cut(s) 449
Eco24I GRGCYC 2 cut(s) 771, 1467
Eco32I GATATC 1 cut(s) 1749
Eco47I GGWCC 1 cut(s) 485
Eco53kI GAGCTC 2 cut(s) 769, 1465
Eco57I CTGAAG 3 cut(s) 290, 1082, 1638
Eco88I CYCGRG 1 cut(s) 402
EcoICRI GAGCTC 2 cut(s) 769, 1465
EcoRII CCWGG 1 cut(s) 1576
EcoRV GATATC 1 cut(s) 1749
EcoT14I CCWWGG 1 cut(s) 449
EcoT22I ATGCAT 1 cut(s) 1461
EcoT38I GRGCYC 2 cut(s) 771, 1467
ErhI CCWWGG 1 cut(s) 449
Esp3I CGTCTC 1 cut(s) 1572
FaeI CATG 7 cut(s) 315, 453, 581, 852, 1025, 1463, 1506
FalI AAGNNNNNCTT 2 cut(s) 1678, 1710
FatI CATG 7 cut(s) 311, 449, 577, 848, 1021, 1459, 1502
FblI GTMKAC 2 cut(s) 96, 1143
Fnu4HI GCNGC 5 cut(s) 38, 648, 1104, 1491, 1764
FokI GGATG 3 cut(s) 687, 1187, 1336
FriOI GRGCYC 2 cut(s) 771, 1467
Fsp4HI GCNGC 5 cut(s) 38, 648, 1104, 1491, 1764
FspBI CTAG 6 cut(s) 203, 386, 479, 830, 1410, 1509
GluI GCNGC 5 cut(s) 38, 648, 1104, 1491, 1764
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 1383
HapII CCGG 1 cut(s) 32
HgaI GACGC 3 cut(s) 90, 653, 934
Hin1II CATG 7 cut(s) 315, 453, 581, 852, 1025, 1463, 1506
HincII GTYRAC 3 cut(s) 97, 132, 714
HindII GTYRAC 3 cut(s) 97, 132, 714
HinfI GANTC 8 cut(s) 55, 758, 980, 1111, 1180, 1339, 1535, 1742
HpaII CCGG 1 cut(s) 32
HphI GGTGA 2 cut(s) 758, 1252
Hpy166II GTNNAC 6 cut(s) 97, 132, 355, 714, 1018, 1144
Hpy188I TCNGA 7 cut(s) 215, 361, 497, 570, 691, 1618, 1699
Hpy8I GTNNAC 6 cut(s) 97, 132, 355, 714, 1018, 1144
Hpy99I CGWCG 3 cut(s) 647, 928, 1548
HpyAV CCTTC 7 cut(s) 270, 492, 713, 748, 1057, 1214, 1325
HpyCH4III ACNGT 5 cut(s) 962, 1141, 1218, 1254, 1268
HpyCH4IV ACGT 5 cut(s) 128, 740, 1129, 1483, 1546
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 941, 1487
HpyF3I CTNAG 6 cut(s) 152, 212, 336, 358, 707, 791
HpySE526I ACGT 5 cut(s) 128, 740, 1129, 1483, 1546
Hsp92II CATG 7 cut(s) 315, 453, 581, 852, 1025, 1463, 1506
Kzo9I GATC 8 cut(s) 208, 315, 379, 492, 529, 859, 912, 1311
LmnI GCTCC 1 cut(s) 896
Lsp1109I GCAGC 5 cut(s) 24, 634, 1090, 1477, 1750
LweI GCATC 4 cut(s) 148, 922, 1209, 1293
MaeI CTAG 6 cut(s) 203, 386, 479, 830, 1410, 1509
MaeII ACGT 5 cut(s) 128, 740, 1129, 1483, 1546
MaeIII GTNAC 5 cut(s) 736, 919, 1056, 1385, 1690
MalI GATC 8 cut(s) 210, 317, 381, 494, 531, 861, 914, 1313
MboI GATC 8 cut(s) 208, 315, 379, 492, 529, 859, 912, 1311
MboII GAAGA 8 cut(s) 382, 401, 436, 719, 1166, 1268, 1367, 1385
MfeI CAATTG 3 cut(s) 138, 1307, 1774
MhlI GDGCHC 2 cut(s) 771, 1467
MluI ACGCGT 2 cut(s) 82, 926
MlyI GAGTC 4 cut(s) 989, 1105, 1529, 1751
MmeI TCCRAC 3 cut(s) 874, 983, 1212
MnlI CCTC 7 cut(s) 38, 55, 157, 335, 1249, 1324, 1510
Mph1103I ATGCAT 1 cut(s) 1461
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 4 cut(s) 87, 821, 1031, 1596
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 32, 1578
MunI CAATTG 3 cut(s) 138, 1307, 1774
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 1 cut(s) 1578
MvnI CGCG 4 cut(s) 84, 928, 1447, 1543
MwoI GCNNNNNNNGC 3 cut(s) 143, 941, 1487
NciI CCSGG 1 cut(s) 32
NcoI CCATGG 1 cut(s) 449
NdeII GATC 8 cut(s) 208, 315, 379, 492, 529, 859, 912, 1311
NlaIII CATG 7 cut(s) 315, 453, 581, 852, 1025, 1463, 1506
NmuCI GTSAC 3 cut(s) 736, 919, 1385
NruI TCGCGA 2 cut(s) 1447, 1543
NsiI ATGCAT 1 cut(s) 1461
NspI RCATGY 1 cut(s) 852
PaeR7I CTCGAG 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 932
PctI GAATGC 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 4 cut(s) 55, 758, 1180, 1339
PkrI GCNGC 5 cut(s) 39, 649, 1105, 1492, 1765
PleI GAGTC 4 cut(s) 988, 1105, 1529, 1750
PpsI GAGTC 4 cut(s) 988, 1105, 1529, 1750
PsiI TTATAA 2 cut(s) 972, 1671
Psp124BI GAGCTC 2 cut(s) 771, 1467
Psp1406I AACGTT 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 1576
PspGI CCWGG 1 cut(s) 1576
PspPI GGNCC 2 cut(s) 485, 1381
PstI CTGCAG 2 cut(s) 724, 1495
RruI TCGCGA 2 cut(s) 1447, 1543
RsaI GTAC 3 cut(s) 74, 556, 598
RsaNI GTAC 3 cut(s) 73, 555, 597
SacI GAGCTC 2 cut(s) 771, 1467
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 4 cut(s) 87, 821, 1031, 1596
SatI GCNGC 5 cut(s) 38, 648, 1104, 1491, 1764
Sau3AI GATC 8 cut(s) 208, 315, 379, 492, 529, 859, 912, 1311
Sau96I GGNCC 2 cut(s) 485, 1381
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 4 cut(s) 989, 1105, 1529, 1751
ScrFI CCNGG 2 cut(s) 32, 1578
SduI GDGCHC 2 cut(s) 771, 1467
SfaNI GCATC 4 cut(s) 148, 922, 1209, 1293
SfcI CTRYAG 2 cut(s) 720, 1491
Sfr274I CTCGAG 1 cut(s) 402
SinI GGWCC 1 cut(s) 485
SlaI CTCGAG 1 cut(s) 402
SmlI CTYRAG 2 cut(s) 41, 402
SmoI CTYRAG 2 cut(s) 41, 402
SsiI CCGC 5 cut(s) 146, 161, 882, 1343, 1633
SspI AATATT 1 cut(s) 1161
SspMI CTAG 6 cut(s) 203, 386, 479, 830, 1410, 1509
SstI GAGCTC 2 cut(s) 771, 1467
StyD4I CCNGG 2 cut(s) 30, 1576
StyI CCWWGG 1 cut(s) 449
TaaI ACNGT 5 cut(s) 962, 1141, 1218, 1254, 1268
TaiI ACGT 5 cut(s) 131, 743, 1132, 1486, 1549
TaqI TCGA 9 cut(s) 58, 96, 382, 403, 699, 983, 1241, 1396, 1745
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 4 cut(s) 55, 758, 1180, 1339
Tru1I TTAA 4 cut(s) 87, 821, 1031, 1596
Tru9I TTAA 4 cut(s) 87, 821, 1031, 1596
TscAI CASTG 2 cut(s) 195, 627
TseFI GTSAC 3 cut(s) 736, 919, 1385
TseI GCWGC 5 cut(s) 37, 647, 1103, 1490, 1763
Tsp45I GTSAC 3 cut(s) 736, 919, 1385
TspDTI ATGAA 4 cut(s) 300, 519, 566, 1491
TspRI CASTG 2 cut(s) 195, 627
VpaK11BI GGWCC 1 cut(s) 485
XapI RAATTY 5 cut(s) 519, 571, 1243, 1292, 1498
XbaI TCTAGA 2 cut(s) 829, 1508
XceI RCATGY 1 cut(s) 852
XhoI CTCGAG 1 cut(s) 402
XmiI GTMKAC 2 cut(s) 96, 1143
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 6 cut(s) 203, 386, 479, 830, 1410, 1509
ZrmI AGTACT 1 cut(s) 74
Zsp2I ATGCAT 1 cut(s) 1461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.