Cyclic nucleotide-gated ion channel 1-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 110, 1873 |
| AccB1I | GGYRCC | 1 cut(s) | 1842 |
| AccI | GTMKAC | 2 cut(s) | 1116, 2369 |
| AciI | CCGC | 4 cut(s) | 235, 1846, 2626, 3241 |
| AclI | AACGTT | 1 cut(s) | 368 |
| AclWI | GGATC | 6 cut(s) | 299, 312, 2192, 3050, 3191, 3204 |
| AcoI | YGGCCR | 1 cut(s) | 1624 |
| AcuI | CTGAAG | 1 cut(s) | 1496 |
| AcyI | GRCGYC | 1 cut(s) | 1843 |
| AfaI | GTAC | 9 cut(s) | 539, 647, 812, 1099, 1991, 2023, 2228, 2847, 2911 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 358, 1107, 1272, 1792, 1813, 1879, 2433, 2625 |
| AjnI | CCWGG | 2 cut(s) | 139, 2200 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1670, 1702 |
| Alw21I | GWGCWC | 5 cut(s) | 991, 1500, 1650, 2689, 3033 |
| Alw26I | GTCTC | 5 cut(s) | 613, 1376, 1515, 1768, 2687 |
| AlwI | GGATC | 6 cut(s) | 299, 312, 2192, 3050, 3191, 3204 |
| Ama87I | CYCGRG | 3 cut(s) | 507, 616, 1365 |
| AoxI | GGCC | 3 cut(s) | 137, 1254, 1624 |
| ApeKI | GCWGC | 4 cut(s) | 755, 1817, 1835, 2678 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 887, 919 |
| AseI | ATTAAT | 1 cut(s) | 245 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 54, 495 |
| AspA2I | CCTAGG | 2 cut(s) | 1075, 2354 |
| AspLEI | GCGC | 3 cut(s) | 1595, 1711, 1845 |
| AspS9I | GGNCC | 4 cut(s) | 137, 1406, 1795, 2276 |
| AsuC2I | CCSGG | 3 cut(s) | 239, 1366, 1367 |
| AsuHPI | GGTGA | 8 cut(s) | 38, 56, 500, 592, 1625, 1955, 1964, 2642 |
| AsuII | TTCGAA | 4 cut(s) | 685, 941, 2254, 3376 |
| AsuNHI | GCTAGC | 1 cut(s) | 911 |
| AvaI | CYCGRG | 3 cut(s) | 507, 616, 1365 |
| AvaII | GGWCC | 3 cut(s) | 1406, 1795, 2276 |
| AvrII | CCTAGG | 2 cut(s) | 1075, 2354 |
| BalI | TGGCCA | 1 cut(s) | 1626 |
| BamHI | GGATCC | 2 cut(s) | 304, 3196 |
| BanI | GGYRCC | 1 cut(s) | 1842 |
| BanII | GRGCYC | 1 cut(s) | 3033 |
| BauI | CACGAG | 2 cut(s) | 1551, 3060 |
| BbsI | GAAGAC | 1 cut(s) | 950 |
| Bbv12I | GWGCWC | 5 cut(s) | 991, 1500, 1650, 2689, 3033 |
| BbvI | GCAGC | 4 cut(s) | 742, 1829, 1847, 2665 |
| BccI | CCATC | 6 cut(s) | 1246, 2035, 2603, 3365, 3379, 3406 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 2933, 2967, 3003, 3037 |
| BciT130I | CCWGG | 2 cut(s) | 141, 2202 |
| BciVI | GTATCC | 2 cut(s) | 9, 2473 |
| BclI | TGATCA | 2 cut(s) | 1468, 2781 |
| BcnI | CCSGG | 3 cut(s) | 239, 1366, 1367 |
| BcoDI | GTCTC | 5 cut(s) | 613, 1376, 1515, 1768, 2687 |
| BfaI | CTAG | 9 cut(s) | 384, 447, 912, 1076, 2132, 2189, 2355, 3155, 3179 |
| BfmI | CTRYAG | 4 cut(s) | 124, 201, 378, 3321 |
| BfoI | RGCGCY | 2 cut(s) | 1596, 1846 |
| BfuI | GTATCC | 2 cut(s) | 9, 2473 |
| BglII | AGATCT | 1 cut(s) | 1032 |
| BisI | GCNGC | 5 cut(s) | 756, 1818, 1836, 1846, 2679 |
| BlnI | CCTAGG | 2 cut(s) | 1075, 2354 |
| BlsI | GCNGC | 5 cut(s) | 757, 1819, 1837, 1847, 2680 |
| Bme1390I | CCNGG | 5 cut(s) | 141, 239, 1366, 1367, 2202 |
| Bme18I | GGWCC | 3 cut(s) | 1406, 1795, 2276 |
| BmeT110I | CYCGRG | 3 cut(s) | 507, 616, 1365 |
| BmgT120I | GGNCC | 4 cut(s) | 137, 1406, 1795, 2276 |
| BmiI | GGNNCC | 6 cut(s) | 138, 306, 498, 802, 1844, 3198 |
| BmrFI | CCNGG | 5 cut(s) | 141, 239, 1366, 1367, 2202 |
| BmrI | ACTGGG | 2 cut(s) | 1651, 1782 |
| BmsI | GCATC | 6 cut(s) | 104, 258, 735, 1416, 1447, 2439 |
| BmtI | GCTAGC | 1 cut(s) | 915 |
| BmuI | ACTGGG | 2 cut(s) | 1651, 1782 |
| BpiI | GAAGAC | 1 cut(s) | 950 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1512, 1544 |
| BpmI | CTGGAG | 2 cut(s) | 1232, 2703 |
| Bpu10I | CCTNAGC | 1 cut(s) | 674 |
| Bpu14I | TTCGAA | 4 cut(s) | 685, 941, 2254, 3376 |
| BpuEI | CTTGAG | 3 cut(s) | 229, 1265, 1634 |
| BpuMI | CCSGG | 3 cut(s) | 239, 1366, 1367 |
| BsaAI | YACGTR | 1 cut(s) | 670 |
| BsaHI | GRCGYC | 1 cut(s) | 1843 |
| BsaI | GGTCTC | 1 cut(s) | 1515 |
| BsaJI | CCNNGG | 7 cut(s) | 352, 1075, 1271, 1364, 1365, 1811, 2354 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2053, 2083 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 358, 1107, 1272, 1792, 1813, 1879, 2433, 2625 |
| Bse1I | ACTGG | 7 cut(s) | 306, 498, 786, 1646, 1788, 2701, 3314 |
| Bse3DI | GCAATG | 1 cut(s) | 1591 |
| BseBI | CCWGG | 2 cut(s) | 141, 2202 |
| BseDI | CCNNGG | 7 cut(s) | 352, 1075, 1271, 1364, 1365, 1811, 2354 |
| BseGI | GGATG | 3 cut(s) | 552, 726, 1257 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 358, 1107, 1272, 1792, 1813, 1879, 2433, 2625 |
| BseMI | GCAATG | 1 cut(s) | 1591 |
| BseMII | CTCAG | 4 cut(s) | 1058, 1137, 1426, 2073 |
| BseNI | ACTGG | 7 cut(s) | 306, 498, 786, 1646, 1788, 2701, 3314 |
| BseXI | GCAGC | 4 cut(s) | 742, 1829, 1847, 2665 |
| BshFI | GGCC | 3 cut(s) | 139, 1256, 1626 |
| BshNI | GGYRCC | 1 cut(s) | 1842 |
| BsiHKAI | GWGCWC | 5 cut(s) | 991, 1500, 1650, 2689, 3033 |
| BsiHKCI | CYCGRG | 3 cut(s) | 507, 616, 1365 |
| BsiSI | CCGG | 2 cut(s) | 238, 1366 |
| BslFI | GGGAC | 3 cut(s) | 1068, 1382, 2087 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 358, 1107, 1272, 1792, 1813, 1879, 2433, 2625 |
| BsmAI | GTCTC | 5 cut(s) | 613, 1376, 1515, 1768, 2687 |
| BsmFI | GGGAC | 3 cut(s) | 1068, 1382, 2087 |
| BsmI | GAATGC | 1 cut(s) | 2365 |
| BsnI | GGCC | 3 cut(s) | 139, 1256, 1626 |
| Bso31I | GGTCTC | 1 cut(s) | 1515 |
| BsoBI | CYCGRG | 3 cut(s) | 507, 616, 1365 |
| Bsp119I | TTCGAA | 4 cut(s) | 685, 941, 2254, 3376 |
| Bsp1286I | GDGCHC | 5 cut(s) | 991, 1500, 1650, 2689, 3033 |
| BspACI | CCGC | 4 cut(s) | 235, 1846, 2626, 3241 |
| BspANI | GGCC | 3 cut(s) | 139, 1256, 1626 |
| BspCNI | CTCAG | 4 cut(s) | 1059, 1136, 1425, 2074 |
| BspHI | TCATGA | 2 cut(s) | 1023, 3124 |
| BspLI | GGNNCC | 6 cut(s) | 138, 306, 498, 802, 1844, 3198 |
| BspOI | GCTAGC | 1 cut(s) | 915 |
| BspPI | GGATC | 6 cut(s) | 299, 312, 2192, 3050, 3191, 3204 |
| BspQI | GCTCTTC | 2 cut(s) | 943, 2251 |
| BspT104I | TTCGAA | 4 cut(s) | 685, 941, 2254, 3376 |
| BspT107I | GGYRCC | 1 cut(s) | 1842 |
| BspTNI | GGTCTC | 1 cut(s) | 1515 |
| BsrDI | GCAATG | 1 cut(s) | 1591 |
| BsrI | ACTGG | 7 cut(s) | 306, 498, 786, 1646, 1788, 2701, 3314 |
| BssECI | CCNNGG | 7 cut(s) | 352, 1075, 1271, 1364, 1365, 1811, 2354 |
| BssNAI | GTATAC | 1 cut(s) | 2370 |
| BssNI | GRCGYC | 1 cut(s) | 1843 |
| BssSI | CACGAG | 2 cut(s) | 1551, 3060 |
| BssT1I | CCWWGG | 4 cut(s) | 352, 1075, 1811, 2354 |
| Bst1107I | GTATAC | 1 cut(s) | 2370 |
| Bst2BI | CACGAG | 2 cut(s) | 1551, 3060 |
| Bst2UI | CCWGG | 2 cut(s) | 141, 2202 |
| Bst4CI | ACNGT | 6 cut(s) | 639, 881, 1155, 1702, 2493, 3223 |
| Bst6I | CTCTTC | 4 cut(s) | 943, 2130, 2251, 2658 |
| BstACI | GRCGYC | 1 cut(s) | 1843 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1823 |
| BstBAI | YACGTR | 1 cut(s) | 670 |
| BstBI | TTCGAA | 4 cut(s) | 685, 941, 2254, 3376 |
| BstC8I | GCNNGC | 8 cut(s) | 783, 845, 867, 913, 1482, 1669, 1833, 3150 |
| BstF5I | GGATG | 3 cut(s) | 552, 726, 1257 |
| BstH2I | RGCGCY | 2 cut(s) | 1596, 1846 |
| BstHHI | GCGC | 3 cut(s) | 1595, 1711, 1845 |
| BstMAI | GTCTC | 5 cut(s) | 613, 1376, 1515, 1768, 2687 |
| BstNI | CCWGG | 2 cut(s) | 141, 2202 |
| BstNSI | RCATGY | 2 cut(s) | 869, 2626 |
| BstSCI | CCNGG | 5 cut(s) | 139, 237, 1364, 1365, 2200 |
| BstSFI | CTRYAG | 4 cut(s) | 124, 201, 378, 3321 |
| BstV1I | GCAGC | 4 cut(s) | 742, 1829, 1847, 2665 |
| BstV2I | GAAGAC | 1 cut(s) | 950 |
| BstX2I | RGATCY | 4 cut(s) | 304, 1032, 3055, 3196 |
| BstYI | RGATCY | 4 cut(s) | 304, 1032, 3055, 3196 |
| BstZ17I | GTATAC | 1 cut(s) | 2370 |
| BsuI | GTATCC | 2 cut(s) | 9, 2473 |
| BsuRI | GGCC | 3 cut(s) | 139, 1256, 1626 |
| BtgZI | GCGATG | 1 cut(s) | 274 |
| BtsCI | GGATG | 3 cut(s) | 552, 726, 1257 |
| BtsI | GCAGTG | 1 cut(s) | 1894 |
| BtsIMutI | CAGTG | 5 cut(s) | 105, 299, 877, 1894, 1899 |
| Cac8I | GCNNGC | 8 cut(s) | 783, 845, 867, 913, 1482, 1669, 1833, 3150 |
| CciI | TCATGA | 2 cut(s) | 1023, 3124 |
| CfoI | GCGC | 3 cut(s) | 1595, 1711, 1845 |
| Cfr13I | GGNCC | 4 cut(s) | 137, 1406, 1795, 2276 |
| Cfr9I | CCCGGG | 1 cut(s) | 1365 |
| CseI | GACGC | 1 cut(s) | 2201 |
| Csp6I | GTAC | 9 cut(s) | 538, 646, 811, 1098, 1990, 2022, 2227, 2846, 2910 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 946, 981 |
| CviQI | GTAC | 9 cut(s) | 538, 646, 811, 1098, 1990, 2022, 2227, 2846, 2910 |
| DinI | GGCGCC | 1 cut(s) | 1844 |
| DraI | TTTAAA | 3 cut(s) | 2109, 2400, 3082 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 110, 1873 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 110, 1873 |
| EaeI | YGGCCR | 1 cut(s) | 1624 |
| Eam1104I | CTCTTC | 4 cut(s) | 943, 2130, 2251, 2658 |
| EarI | CTCTTC | 4 cut(s) | 943, 2130, 2251, 2658 |
| Ecl136II | GAGCTC | 1 cut(s) | 3031 |
| Eco130I | CCWWGG | 4 cut(s) | 352, 1075, 1811, 2354 |
| Eco24I | GRGCYC | 1 cut(s) | 3033 |
| Eco31I | GGTCTC | 1 cut(s) | 1515 |
| Eco47I | GGWCC | 3 cut(s) | 1406, 1795, 2276 |
| Eco53kI | GAGCTC | 1 cut(s) | 3031 |
| Eco57I | CTGAAG | 1 cut(s) | 1496 |
| Eco88I | CYCGRG | 3 cut(s) | 507, 616, 1365 |
| EcoICRI | GAGCTC | 1 cut(s) | 3031 |
| EcoO109I | RGGNCCY | 1 cut(s) | 137 |
| EcoRI | GAATTC | 1 cut(s) | 2086 |
| EcoRII | CCWGG | 2 cut(s) | 139, 2200 |
| EcoT14I | CCWWGG | 4 cut(s) | 352, 1075, 1811, 2354 |
| EcoT22I | ATGCAT | 1 cut(s) | 867 |
| EcoT38I | GRGCYC | 1 cut(s) | 3033 |
| EgeI | GGCGCC | 1 cut(s) | 1844 |
| EheI | GGCGCC | 1 cut(s) | 1844 |
| ErhI | CCWWGG | 4 cut(s) | 352, 1075, 1811, 2354 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1601, 1633, 1620, 1652 |
| FaqI | GGGAC | 3 cut(s) | 1068, 1382, 2087 |
| FauI | CCCGC | 1 cut(s) | 2619 |
| FauNDI | CATATG | 1 cut(s) | 3139 |
| FbaI | TGATCA | 2 cut(s) | 1468, 2781 |
| FblI | GTMKAC | 2 cut(s) | 1116, 2369 |
| Fnu4HI | GCNGC | 5 cut(s) | 756, 1818, 1836, 1846, 2679 |
| FokI | GGATG | 3 cut(s) | 539, 713, 1264 |
| FriOI | GRGCYC | 1 cut(s) | 3033 |
| Fsp4HI | GCNGC | 5 cut(s) | 756, 1818, 1836, 1846, 2679 |
| FspBI | CTAG | 9 cut(s) | 384, 447, 912, 1076, 2132, 2189, 2355, 3155, 3179 |
| GlaI | GCGC | 3 cut(s) | 1594, 1710, 1844 |
| GluI | GCNGC | 5 cut(s) | 756, 1818, 1836, 1846, 2679 |
| GsuI | CTGGAG | 2 cut(s) | 1232, 2703 |
| HaeII | RGCGCY | 2 cut(s) | 1596, 1846 |
| HaeIII | GGCC | 3 cut(s) | 139, 1256, 1626 |
| HapII | CCGG | 2 cut(s) | 238, 1366 |
| HgaI | GACGC | 1 cut(s) | 2201 |
| HhaI | GCGC | 3 cut(s) | 1595, 1711, 1845 |
| Hin1I | GRCGYC | 1 cut(s) | 1843 |
| Hin6I | GCGC | 3 cut(s) | 1593, 1709, 1843 |
| HinP1I | GCGC | 3 cut(s) | 1593, 1709, 1843 |
| HindIII | AAGCTT | 1 cut(s) | 2649 |
| HpaII | CCGG | 2 cut(s) | 238, 1366 |
| HphI | GGTGA | 8 cut(s) | 38, 56, 500, 592, 1625, 1955, 1964, 2642 |
| Hpy188I | TCNGA | 8 cut(s) | 924, 1275, 1376, 1425, 1476, 1515, 3192, 3229 |
| HpyCH4III | ACNGT | 6 cut(s) | 639, 881, 1155, 1702, 2493, 3223 |
| HpyCH4IV | ACGT | 3 cut(s) | 368, 669, 2194 |
| HpySE526I | ACGT | 3 cut(s) | 368, 669, 2194 |
| Hsp92I | GRCGYC | 1 cut(s) | 1843 |
| HspAI | GCGC | 3 cut(s) | 1593, 1709, 1843 |
| KasI | GGCGCC | 1 cut(s) | 1842 |
| Ksp22I | TGATCA | 2 cut(s) | 1468, 2781 |
| LguI | GCTCTTC | 2 cut(s) | 943, 2251 |
| LmnI | GCTCC | 9 cut(s) | 374, 800, 908, 2356, 2684, 2795, 2899, 3028, 3036 |
| Lsp1109I | GCAGC | 4 cut(s) | 742, 1829, 1847, 2665 |
| LweI | GCATC | 6 cut(s) | 104, 258, 735, 1416, 1447, 2439 |
| MaeI | CTAG | 9 cut(s) | 384, 447, 912, 1076, 2132, 2189, 2355, 3155, 3179 |
| MaeII | ACGT | 3 cut(s) | 368, 669, 2194 |
| MaeIII | GTNAC | 6 cut(s) | 454, 893, 1613, 2077, 2243, 2411 |
| MfeI | CAATTG | 1 cut(s) | 2295 |
| MflI | RGATCY | 4 cut(s) | 304, 1032, 3055, 3196 |
| MhlI | GDGCHC | 5 cut(s) | 991, 1500, 1650, 2689, 3033 |
| MlsI | TGGCCA | 1 cut(s) | 1626 |
| MluNI | TGGCCA | 1 cut(s) | 1626 |
| Mly113I | GGCGCC | 1 cut(s) | 1843 |
| MlyI | GAGTC | 5 cut(s) | 98, 923, 1861, 2346, 3301 |
| MmeI | TCCRAC | 4 cut(s) | 778, 1701, 2979, 3006 |
| Mox20I | TGGCCA | 1 cut(s) | 1626 |
| Mph1103I | ATGCAT | 1 cut(s) | 867 |
| MroXI | GAANNNNTTC | 2 cut(s) | 54, 495 |
| MscI | TGGCCA | 1 cut(s) | 1626 |
| MslI | CAYNNNNRTG | 2 cut(s) | 81, 3082 |
| Msp20I | TGGCCA | 1 cut(s) | 1626 |
| MspI | CCGG | 2 cut(s) | 238, 1366 |
| MspR9I | CCNGG | 5 cut(s) | 141, 239, 1366, 1367, 2202 |
| MunI | CAATTG | 1 cut(s) | 2295 |
| Mva1269I | GAATGC | 1 cut(s) | 2365 |
| MvaI | CCWGG | 2 cut(s) | 141, 2202 |
| NarI | GGCGCC | 1 cut(s) | 1843 |
| NciI | CCSGG | 3 cut(s) | 239, 1366, 1367 |
| NdeI | CATATG | 1 cut(s) | 3139 |
| NheI | GCTAGC | 1 cut(s) | 911 |
| NlaIV | GGNNCC | 6 cut(s) | 138, 306, 498, 802, 1844, 3198 |
| NmeAIII | GCCGAG | 1 cut(s) | 86 |
| NmuCI | GTSAC | 5 cut(s) | 454, 893, 1613, 2077, 2243 |
| NsiI | ATGCAT | 1 cut(s) | 867 |
| NspI | RCATGY | 2 cut(s) | 869, 2626 |
| NspV | TTCGAA | 4 cut(s) | 685, 941, 2254, 3376 |
| PaeI | GCATGC | 1 cut(s) | 869 |
| PaeR7I | CTCGAG | 1 cut(s) | 616 |
| PagI | TCATGA | 2 cut(s) | 1023, 3124 |
| PciSI | GCTCTTC | 2 cut(s) | 943, 2251 |
| PctI | GAATGC | 1 cut(s) | 2365 |
| PdmI | GAANNNNTTC | 2 cut(s) | 54, 495 |
| PfeI | GAWTC | 6 cut(s) | 687, 995, 1010, 2303, 2430, 3251 |
| PfoI | TCCNGGA | 1 cut(s) | 2200 |
| PkrI | GCNGC | 5 cut(s) | 757, 1819, 1837, 1847, 2680 |
| PleI | GAGTC | 5 cut(s) | 98, 923, 1861, 2345, 3301 |
| PluTI | GGCGCC | 1 cut(s) | 1846 |
| PpsI | GAGTC | 5 cut(s) | 98, 923, 1861, 2345, 3301 |
| Ppu21I | YACGTR | 1 cut(s) | 670 |
| PshBI | ATTAAT | 1 cut(s) | 245 |
| Psp124BI | GAGCTC | 1 cut(s) | 3033 |
| Psp1406I | AACGTT | 1 cut(s) | 368 |
| Psp6I | CCWGG | 2 cut(s) | 139, 2200 |
| PspGI | CCWGG | 2 cut(s) | 139, 2200 |
| PspN4I | GGNNCC | 6 cut(s) | 138, 306, 498, 802, 1844, 3198 |
| PspPI | GGNCC | 4 cut(s) | 137, 1406, 1795, 2276 |
| PsuI | RGATCY | 4 cut(s) | 304, 1032, 3055, 3196 |
| RsaI | GTAC | 9 cut(s) | 539, 647, 812, 1099, 1991, 2023, 2228, 2847, 2911 |
| RsaNI | GTAC | 9 cut(s) | 538, 646, 811, 1098, 1990, 2022, 2227, 2846, 2910 |
| RseI | CAYNNNNRTG | 2 cut(s) | 81, 3082 |
| SacI | GAGCTC | 1 cut(s) | 3033 |
| SapI | GCTCTTC | 2 cut(s) | 943, 2251 |
| SatI | GCNGC | 5 cut(s) | 756, 1818, 1836, 1846, 2679 |
| Sau96I | GGNCC | 4 cut(s) | 137, 1406, 1795, 2276 |
| SchI | GAGTC | 5 cut(s) | 98, 923, 1861, 2346, 3301 |
| ScrFI | CCNGG | 5 cut(s) | 141, 239, 1366, 1367, 2202 |
| SduI | GDGCHC | 5 cut(s) | 991, 1500, 1650, 2689, 3033 |
| SfaNI | GCATC | 6 cut(s) | 104, 258, 735, 1416, 1447, 2439 |
| SfcI | CTRYAG | 4 cut(s) | 124, 201, 378, 3321 |
| SfoI | GGCGCC | 1 cut(s) | 1844 |
| Sfr274I | CTCGAG | 1 cut(s) | 616 |
| SfuI | TTCGAA | 4 cut(s) | 685, 941, 2254, 3376 |
| SinI | GGWCC | 3 cut(s) | 1406, 1795, 2276 |
| SlaI | CTCGAG | 1 cut(s) | 616 |
| SmaI | CCCGGG | 1 cut(s) | 1367 |
| SmiI | ATTTAAAT | 1 cut(s) | 3082 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 81, 3082 |
| SmlI | CTYRAG | 4 cut(s) | 208, 616, 1280, 1649 |
| SmoI | CTYRAG | 4 cut(s) | 208, 616, 1280, 1649 |
| SphI | GCATGC | 1 cut(s) | 869 |
| SsiI | CCGC | 4 cut(s) | 235, 1846, 2626, 3241 |
| SspDI | GGCGCC | 1 cut(s) | 1842 |
| SspI | AATATT | 3 cut(s) | 2251, 2323, 2932 |
| SspMI | CTAG | 9 cut(s) | 384, 447, 912, 1076, 2132, 2189, 2355, 3155, 3179 |
| SstI | GAGCTC | 1 cut(s) | 3033 |
| StyD4I | CCNGG | 5 cut(s) | 139, 237, 1364, 1365, 2200 |
| StyI | CCWWGG | 4 cut(s) | 352, 1075, 1811, 2354 |
| SwaI | ATTTAAAT | 1 cut(s) | 3082 |
| TaaI | ACNGT | 6 cut(s) | 639, 881, 1155, 1702, 2493, 3223 |
| TaiI | ACGT | 3 cut(s) | 371, 672, 2197 |
| TatI | WGTACW | 4 cut(s) | 537, 645, 2845, 2909 |
| TauI | GCSGC | 1 cut(s) | 1848 |
| TfiI | GAWTC | 6 cut(s) | 687, 995, 1010, 2303, 2430, 3251 |
| TscAI | CASTG | 5 cut(s) | 105, 306, 884, 1894, 1906 |
| TseFI | GTSAC | 5 cut(s) | 454, 893, 1613, 2077, 2243 |
| TseI | GCWGC | 4 cut(s) | 755, 1817, 1835, 2678 |
| Tsp45I | GTSAC | 5 cut(s) | 454, 893, 1613, 2077, 2243 |
| TspMI | CCCGGG | 1 cut(s) | 1365 |
| TspRI | CASTG | 5 cut(s) | 105, 306, 884, 1894, 1906 |
| VpaK11BI | GGWCC | 3 cut(s) | 1406, 1795, 2276 |
| VspI | ATTAAT | 1 cut(s) | 245 |
| XbaI | TCTAGA | 2 cut(s) | 2188, 3154 |
| XceI | RCATGY | 2 cut(s) | 869, 2626 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1913 |
| XhoI | CTCGAG | 1 cut(s) | 616 |
| XmaI | CCCGGG | 1 cut(s) | 1365 |
| XmaJI | CCTAGG | 2 cut(s) | 1075, 2354 |
| XmiI | GTMKAC | 2 cut(s) | 1116, 2369 |
| XmnI | GAANNNNTTC | 2 cut(s) | 54, 495 |
| XspI | CTAG | 9 cut(s) | 384, 447, 912, 1076, 2132, 2189, 2355, 3155, 3179 |
| Zsp2I | ATGCAT | 1 cut(s) | 867 |