Rorug01G0420100

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000755
Physical Location & Seq
Reverse (-)
52169666 .. 52171907
2242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0420100.1

Sequence Viewer

Length: 1527 bp
ATGGCGGTCGGAGTAGCACTAACAGGTGAAGGTAGACAATACAATGGCAAGGTGACACCATTTGTCATCCTCTCTTGTATGATGGCCGCCACAGGAGGTGTTATTTTCGGCTACGATATTGGAATTTCAGGTGGAGTGACATCAATGGAGCCATTTCTCGAAAAGTTCTTCCCGGAGGTATACAGGCGGATGAAATCGGACACTAAAATCAGCAACTACTGTACATTTGATAGCGAGCTGTTGACCTCCTTCACATCATCACTATATATAGCTGGTCTTGCAGCTTCTATTTTTGCCAGTTCCGTCACTAGAGCTTATGGCCGCAAGCCTTCGATCCTTGCCGGCGGTGCTGCATTCCTCGCCGGTTCTGCTCTAAACGGTGCGGCTGTGAACATTTACATGCTGATCCTAGGACGCATCTTGCTTGGTGTTGGGGTTGGTTTTGCAAATCAGTCTGTTCCTTTATATCTTTCAGAGATGGCACCATCAAAATATAGAGGGGCAATTACCAATGGCTTCCAATTTAGTGTTGGCATTGGTGCATTATCAGCTAACCTCATCAATTACGGCACTGAAAAGATCGAAGGAGGTTGGGGGTGGCGAATCTCCCTAGCCCTTGCTGCAGTCCCAGCCTCAATCTTAACACTCGGCGCAATTTTCCTTCCGGAAACACCCAACAGTCTAATTCAGCGCAGTGTAGACCACCAAACAGCTATGCTAATGTTGCGGCGCATCAGAGGTGTCGAAGATGTCCAATTAGAACTTGAAGATCTCATCAAAGCAAACAACATTTCCAAAACCATCCAACACCCTTTTAGGAAGATCCTAGAGAGAAGGTATAGGCCTCAACTTGTAATGGCAATAGCCATCCCATTTTTCCAGCAAGTGACTGGAATCAATGTCATAGCTTTCTATGCCCCTATACTCTTTCGAACAATTGGCTTAGGTGAAAGTGCATCACTTTTGTCTGCTGTCATGACTGGTGTAGTCGGTACAATCACAACCTTCATATCCATGCTTATAGTGGACAAGTTTGGCAGAAGGGTCTTGTTCATAGTTGGGGGCCTTCAAATGTTGGTGTCACAAATGATGATTGGGGGAGTCATGGCGACTCAACTAGGTGATCATGGTGGAGTGAGCAAAGGATATGCATATTTGGTACTGGTTTTGATTGGCATATACGTAGCCGGGTTTGGGTGGTCTTGGGGACCTCTAGGATGGTTGGTTCCTAGTGAAATTTTCCCACTGGAGATTAGGTCAGCTGGGCAAAGTATCAATGTGGTGGTGAACTTTTTGTTCACTTTTATTGTTGCTCAAACTTTTCTAGCTATGCTTTGCCACTTCAAGGCTGGGATTTTCTTCTTTTTCGGAGGTTGGGTGATGGTGATGACACTGTTTGTGTACTTCTTTTTGCCGGAGACGAAGAATGTGCCGATTGAGAAGATGGAGACAGTGTGGGTGGAGCACTGGTTTTGGAGGAGAATTGTTGGGGAGTTTGGCGAGGAGGACACCAAGAATGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

508

Amino Acids

55.51

Weight (kDa)

8.95

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 26 - 486 4e-115 Sugar (and other) transporter
MFS_1 PF07690 80 - 436 1e-21 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 481
AccI GTMKAC 3 cut(s) 34, 180, 699
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 7 cut(s) 5, 87, 187, 322, 345, 383, 727
AclWI GGATC 3 cut(s) 328, 400, 817
AcoI YGGCCR 2 cut(s) 84, 319
AcsI RAATTY 2 cut(s) 123, 1236
AfaI GTAC 4 cut(s) 223, 994, 1161, 1403
AfiI CCNNNNNNNGG 2 cut(s) 1194, 1345
AgsI TTSAA 3 cut(s) 767, 1070, 1345
AloI GAACNNNNNNTCC 2 cut(s) 1209, 1241
Alw21I GWGCWC 1 cut(s) 1467
Alw26I GTCTC 2 cut(s) 1412, 1442
AlwI GGATC 3 cut(s) 328, 400, 817
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 4 cut(s) 84, 319, 842, 1063
ApeKI GCWGC 3 cut(s) 281, 350, 620
ApoI RAATTY 2 cut(s) 123, 1236
AspA2I CCTAGG 1 cut(s) 409
AspLEI GCGC 3 cut(s) 653, 693, 732
AspS9I GGNCC 2 cut(s) 1063, 1208
AsuC2I CCSGG 2 cut(s) 173, 1189
AsuHPI GGTGA 7 cut(s) 38, 64, 959, 1133, 1297, 1390, 1396
AsuII TTCGAA 1 cut(s) 931
AvaII GGWCC 1 cut(s) 1208
AvrII CCTAGG 1 cut(s) 409
BanI GGYRCC 1 cut(s) 481
Bbv12I GWGCWC 1 cut(s) 1467
BbvI GCAGC 3 cut(s) 293, 337, 607
BccI CCATC 8 cut(s) 76, 472, 493, 809, 875, 1212, 1375, 1438
BceAI ACGGC 1 cut(s) 583
BcgI CGANNNNNNTGC 2 cut(s) 1411, 1445
BclI TGATCA 1 cut(s) 1123
BcnI CCSGG 2 cut(s) 173, 1189
BcoDI GTCTC 2 cut(s) 1412, 1442
BfaI CTAG 8 cut(s) 309, 410, 611, 827, 1118, 1214, 1230, 1325
BfmI CTRYAG 1 cut(s) 621
BglII AGATCT 1 cut(s) 769
BisI GCNGC 7 cut(s) 87, 282, 322, 351, 384, 621, 728
BlnI CCTAGG 1 cut(s) 409
BlsI GCNGC 7 cut(s) 88, 283, 323, 352, 385, 622, 729
Bme1390I CCNGG 2 cut(s) 173, 1189
Bme18I GGWCC 1 cut(s) 1208
BmgT120I GGNCC 2 cut(s) 1063, 1208
BmiI GGNNCC 5 cut(s) 150, 483, 1064, 1209, 1227
BmrFI CCNGG 2 cut(s) 173, 1189
BmsI GCATC 3 cut(s) 426, 741, 965
BpmI CTGGAG 1 cut(s) 1268
Bpu10I CCTNAGC 1 cut(s) 943
Bpu14I TTCGAA 1 cut(s) 931
BpuMI CCSGG 2 cut(s) 173, 1189
BsaAI YACGTR 1 cut(s) 1183
BsaJI CCNNGG 1 cut(s) 409
BsaWI WCCGGW 1 cut(s) 664
BsaXI ACNNNNNCTCC 8 cut(s) 1241, 1271, 1362, 1392, 1439, 1454, 1469, 1484
Bsc4I CCNNNNNNNGG 2 cut(s) 1194, 1345
Bse118I RCCGGY 2 cut(s) 341, 362
Bse1I ACTGG 6 cut(s) 297, 895, 985, 1167, 1251, 1472
BseAI TCCGGA 1 cut(s) 664
BseDI CCNNGG 1 cut(s) 409
BseGI GGATG 5 cut(s) 66, 195, 801, 867, 1223
BseLI CCNNNNNNNGG 2 cut(s) 1194, 1345
BseNI ACTGG 6 cut(s) 297, 895, 985, 1167, 1251, 1472
BseRI GAGGAG 2 cut(s) 1492, 1517
BseXI GCAGC 3 cut(s) 293, 337, 607
BseYI CCCAGC 3 cut(s) 628, 1262, 1349
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 4 cut(s) 86, 321, 844, 1065
BshNI GGYRCC 1 cut(s) 481
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 1467
BsiSI CCGG 6 cut(s) 173, 342, 363, 665, 1188, 1415
BslFI GGGAC 2 cut(s) 611, 1221
BslI CCNNNNNNNGG 2 cut(s) 1194, 1345
BsmAI GTCTC 2 cut(s) 1412, 1442
BsmBI CGTCTC 1 cut(s) 1412
BsmFI GGGAC 2 cut(s) 611, 1221
BsmI GAATGC 1 cut(s) 353
BsnI GGCC 4 cut(s) 86, 321, 844, 1065
Bsp119I TTCGAA 1 cut(s) 931
Bsp1286I GDGCHC 1 cut(s) 1467
Bsp13I TCCGGA 1 cut(s) 664
Bsp1407I TGTACA 1 cut(s) 221
Bsp143I GATC 6 cut(s) 333, 405, 579, 769, 822, 1123
BspACI CCGC 7 cut(s) 5, 87, 187, 322, 345, 383, 727
BspANI GGCC 4 cut(s) 86, 321, 844, 1065
BspEI TCCGGA 1 cut(s) 664
BspHI TCATGA 1 cut(s) 975
BspLI GGNNCC 5 cut(s) 150, 483, 1064, 1209, 1227
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 3 cut(s) 328, 400, 817
BspT104I TTCGAA 1 cut(s) 931
BspT107I GGYRCC 1 cut(s) 481
BsrFI RCCGGY 2 cut(s) 341, 362
BsrGI TGTACA 1 cut(s) 221
BsrI ACTGG 6 cut(s) 297, 895, 985, 1167, 1251, 1472
BssAI RCCGGY 2 cut(s) 341, 362
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 6 cut(s) 333, 405, 579, 769, 822, 1123
BssNAI GTATAC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 409
Bst1107I GTATAC 1 cut(s) 181
Bst4CI ACNGT 5 cut(s) 221, 380, 680, 1395, 1453
BstAUI TGTACA 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 1183
BstBI TTCGAA 1 cut(s) 931
BstC8I GCNNGC 3 cut(s) 236, 326, 343
BstDEI CTNAG 1 cut(s) 943
BstF5I GGATG 5 cut(s) 66, 195, 801, 867, 1223
BstHHI GCGC 3 cut(s) 653, 693, 732
BstKTI GATC 6 cut(s) 336, 408, 582, 772, 825, 1126
BstMAI GTCTC 2 cut(s) 1412, 1442
BstMBI GATC 6 cut(s) 333, 405, 579, 769, 822, 1123
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 9 cut(s) 278, 347, 359, 368, 548, 620, 629, 724, 914
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 2 cut(s) 171, 1187
BstSFI CTRYAG 1 cut(s) 621
BstSNI TACGTA 1 cut(s) 1183
BstV1I GCAGC 3 cut(s) 293, 337, 607
BstX2I RGATCY 2 cut(s) 769, 822
BstYI RGATCY 2 cut(s) 769, 822
BstZ17I GTATAC 1 cut(s) 181
BsuRI GGCC 4 cut(s) 86, 321, 844, 1065
BtsCI GGATG 5 cut(s) 66, 195, 801, 867, 1223
BtsI GCAGTG 1 cut(s) 700
BtsIMutI CAGTG 6 cut(s) 570, 700, 1244, 1391, 1458, 1465
Cac8I GCNNGC 3 cut(s) 236, 326, 343
CciI TCATGA 1 cut(s) 975
CfoI GCGC 3 cut(s) 653, 693, 732
Cfr10I RCCGGY 2 cut(s) 341, 362
Cfr13I GGNCC 2 cut(s) 1063, 1208
CseI GACGC 1 cut(s) 423
Csp6I GTAC 4 cut(s) 222, 993, 1160, 1402
CviAII CATG 5 cut(s) 400, 976, 1015, 1105, 1127
CviQI GTAC 4 cut(s) 222, 993, 1160, 1402
DdeI CTNAG 1 cut(s) 943
DpnI GATC 6 cut(s) 335, 407, 581, 771, 824, 1125
DpnII GATC 6 cut(s) 333, 405, 579, 769, 822, 1123
EaeI YGGCCR 2 cut(s) 84, 319
EciI GGCGGA 1 cut(s) 202
Eco105I TACGTA 1 cut(s) 1183
Eco130I CCWWGG 1 cut(s) 409
Eco147I AGGCCT 1 cut(s) 844
Eco47I GGWCC 1 cut(s) 1208
EcoO109I RGGNCCY 2 cut(s) 1063, 1208
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 1153
ErhI CCWWGG 1 cut(s) 409
Esp3I CGTCTC 1 cut(s) 1412
FaeI CATG 5 cut(s) 403, 979, 1018, 1108, 1130
FaqI GGGAC 2 cut(s) 611, 1221
FatI CATG 5 cut(s) 399, 975, 1014, 1104, 1126
FbaI TGATCA 1 cut(s) 1123
FblI GTMKAC 3 cut(s) 34, 180, 699
Fnu4HI GCNGC 7 cut(s) 87, 282, 322, 351, 384, 621, 728
FokI GGATG 5 cut(s) 53, 202, 788, 854, 1230
Fsp4HI GCNGC 7 cut(s) 87, 282, 322, 351, 384, 621, 728
FspBI CTAG 8 cut(s) 309, 410, 611, 827, 1118, 1214, 1230, 1325
GlaI GCGC 3 cut(s) 652, 692, 731
GluI GCNGC 7 cut(s) 87, 282, 322, 351, 384, 621, 728
GsaI CCCAGC 3 cut(s) 632, 1266, 1353
GsuI CTGGAG 1 cut(s) 1268
HaeIII GGCC 4 cut(s) 86, 321, 844, 1065
HapII CCGG 6 cut(s) 173, 342, 363, 665, 1188, 1415
HgaI GACGC 1 cut(s) 423
HhaI GCGC 3 cut(s) 653, 693, 732
Hin1II CATG 5 cut(s) 403, 979, 1018, 1108, 1130
Hin6I GCGC 3 cut(s) 651, 691, 730
HinP1I GCGC 3 cut(s) 651, 691, 730
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HindIII AAGCTT 1 cut(s) 1521
HinfI GANTC 4 cut(s) 603, 894, 1101, 1111
HpaII CCGG 6 cut(s) 173, 342, 363, 665, 1188, 1415
HphI GGTGA 7 cut(s) 38, 64, 959, 1133, 1297, 1390, 1396
Hpy166II GTNNAC 9 cut(s) 35, 181, 243, 391, 700, 1027, 1288, 1299, 1402
Hpy188I TCNGA 5 cut(s) 11, 199, 475, 737, 1370
Hpy188III TCNNGA 3 cut(s) 158, 665, 976
Hpy8I GTNNAC 9 cut(s) 35, 181, 243, 391, 700, 1027, 1288, 1299, 1402
HpyAV CCTTC 9 cut(s) 23, 259, 339, 578, 671, 828, 1015, 1035, 1076
HpyCH4III ACNGT 5 cut(s) 221, 380, 680, 1395, 1453
HpyCH4IV ACGT 1 cut(s) 1182
HpyCH4V TGCA 7 cut(s) 281, 353, 446, 542, 623, 956, 1151
HpyF10VI GCNNNNNNNGC 9 cut(s) 278, 347, 359, 368, 548, 620, 629, 724, 914
HpyF3I CTNAG 1 cut(s) 943
HpySE526I ACGT 1 cut(s) 1182
Hsp92II CATG 5 cut(s) 403, 979, 1018, 1108, 1130
HspAI GCGC 3 cut(s) 651, 691, 730
Kpn2I TCCGGA 1 cut(s) 664
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
Ksp22I TGATCA 1 cut(s) 1123
Kzo9I GATC 6 cut(s) 333, 405, 579, 769, 822, 1123
LmnI GCTCC 2 cut(s) 148, 1462
Lsp1109I GCAGC 3 cut(s) 293, 337, 607
LweI GCATC 3 cut(s) 426, 741, 965
MaeI CTAG 8 cut(s) 309, 410, 611, 827, 1118, 1214, 1230, 1325
MaeII ACGT 1 cut(s) 1182
MaeIII GTNAC 5 cut(s) 52, 136, 304, 886, 1080
MalI GATC 6 cut(s) 335, 407, 581, 771, 824, 1125
MboI GATC 6 cut(s) 333, 405, 579, 769, 822, 1123
MboII GAAGA 7 cut(s) 160, 758, 779, 832, 1351, 1435, 1453
MfeI CAATTG 1 cut(s) 936
MflI RGATCY 2 cut(s) 769, 822
MhlI GDGCHC 1 cut(s) 1467
MlyI GAGTC 2 cut(s) 1105, 1110
MmeI TCCRAC 1 cut(s) 829
Mph1103I ATGCAT 1 cut(s) 1153
MroI TCCGGA 1 cut(s) 664
MroNI GCCGGC 1 cut(s) 341
MseI TTAA 1 cut(s) 641
MslI CAYNNNNRTG 2 cut(s) 398, 1013
MspA1I CMGCKG 1 cut(s) 1262
MspI CCGG 6 cut(s) 173, 342, 363, 665, 1188, 1415
MspR9I CCNGG 2 cut(s) 173, 1189
MunI CAATTG 1 cut(s) 936
Mva1269I GAATGC 1 cut(s) 353
MwoI GCNNNNNNNGC 9 cut(s) 278, 347, 359, 368, 548, 620, 629, 724, 914
NaeI GCCGGC 1 cut(s) 343
NciI CCSGG 2 cut(s) 173, 1189
NdeII GATC 6 cut(s) 333, 405, 579, 769, 822, 1123
NgoMIV GCCGGC 1 cut(s) 341
NlaIII CATG 5 cut(s) 403, 979, 1018, 1108, 1130
NlaIV GGNNCC 5 cut(s) 150, 483, 1064, 1209, 1227
NmeAIII GCCGAG 1 cut(s) 627
NmuCI GTSAC 5 cut(s) 52, 136, 304, 886, 1080
NsiI ATGCAT 1 cut(s) 1153
NspI RCATGY 1 cut(s) 403
NspV TTCGAA 1 cut(s) 931
PagI TCATGA 1 cut(s) 975
PceI AGGCCT 1 cut(s) 844
PctI GAATGC 1 cut(s) 353
PdiI GCCGGC 1 cut(s) 343
PfeI GAWTC 2 cut(s) 603, 894
PfoI TCCNGGA 1 cut(s) 171
PkrI GCNGC 7 cut(s) 88, 283, 323, 352, 385, 622, 729
PleI GAGTC 2 cut(s) 1105, 1109
PpsI GAGTC 2 cut(s) 1105, 1109
Ppu21I YACGTR 1 cut(s) 1183
PpuMI RGGWCCY 1 cut(s) 1208
Psp5II RGGWCCY 1 cut(s) 1208
PspFI CCCAGC 3 cut(s) 628, 1262, 1349
PspN4I GGNNCC 5 cut(s) 150, 483, 1064, 1209, 1227
PspPI GGNCC 2 cut(s) 1063, 1208
PspPPI RGGWCCY 1 cut(s) 1208
PstI CTGCAG 1 cut(s) 625
PsuI RGATCY 2 cut(s) 769, 822
PvuII CAGCTG 1 cut(s) 1262
RsaI GTAC 4 cut(s) 223, 994, 1161, 1403
RsaNI GTAC 4 cut(s) 222, 993, 1160, 1402
RseI CAYNNNNRTG 2 cut(s) 398, 1013
SaqAI TTAA 1 cut(s) 641
SatI GCNGC 7 cut(s) 87, 282, 322, 351, 384, 621, 728
Sau3AI GATC 6 cut(s) 333, 405, 579, 769, 822, 1123
Sau96I GGNCC 2 cut(s) 1063, 1208
SchI GAGTC 2 cut(s) 1105, 1110
ScrFI CCNGG 2 cut(s) 173, 1189
SduI GDGCHC 1 cut(s) 1467
SfaNI GCATC 3 cut(s) 426, 741, 965
SfcI CTRYAG 1 cut(s) 621
SfuI TTCGAA 1 cut(s) 931
SinI GGWCC 1 cut(s) 1208
SmiMI CAYNNNNRTG 2 cut(s) 398, 1013
SnaBI TACGTA 1 cut(s) 1183
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 7 cut(s) 5, 87, 187, 322, 345, 383, 727
SspMI CTAG 8 cut(s) 309, 410, 611, 827, 1118, 1214, 1230, 1325
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 2 cut(s) 171, 1187
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 5 cut(s) 221, 380, 680, 1395, 1453
TaiI ACGT 1 cut(s) 1185
TaqI TCGA 5 cut(s) 159, 332, 582, 744, 931
TatI WGTACW 2 cut(s) 221, 1401
TauI GCSGC 4 cut(s) 89, 324, 386, 730
TfiI GAWTC 2 cut(s) 603, 894
Tru1I TTAA 1 cut(s) 641
Tru9I TTAA 1 cut(s) 641
TscAI CASTG 6 cut(s) 577, 700, 1251, 1398, 1458, 1472
TseFI GTSAC 5 cut(s) 52, 136, 304, 886, 1080
TseI GCWGC 3 cut(s) 281, 350, 620
Tsp45I GTSAC 5 cut(s) 52, 136, 304, 886, 1080
TspDTI ATGAA 3 cut(s) 206, 997, 1042
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 6 cut(s) 577, 700, 1251, 1398, 1458, 1472
VpaK11BI GGWCC 1 cut(s) 1208
XapI RAATTY 2 cut(s) 123, 1236
XceI RCATGY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 4 cut(s) 527, 887, 1021, 1346
XmaJI CCTAGG 1 cut(s) 409
XmiI GTMKAC 3 cut(s) 34, 180, 699
XspI CTAG 8 cut(s) 309, 410, 611, 827, 1118, 1214, 1230, 1325
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.