Rroxscaffold_1G00023820

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000755
Physical Location & Seq
Reverse (-)
29460275 .. 29463696
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00023820.1

Sequence Viewer

Length: 1641 bp
ATGTTTAGGATACAGAGTGGTGATTTAGAAGAAAATGCTCCCGGAAGTGTATCTTCAGGGGAATCTTTGTCGGCAGGGCACTTCAATAGAGTTTTGGGACCTAGATCTTTTGAAAGGGTAATGAGTACCAAAGGATTAAAATCTCATTGTGGAGTATTGGTTGACAAGCTAAAGCTAATCAGGAAGAATAGAATACTATTTTGGTCTCTTAGCGCGCTCTTACTGGATCCACTTTTCTGTTATATCCTTGTCATGAGTGATGAGAAGAAGTGCATTAGATTGGACAGAATATTGGGGATTATAGCTACTGTTTTGCGATCCCTCAGTGACCTCTACTACATATTTCATATCATCAATCATTTCGATGCTAGATTTGTTACTCGTTCAGTTGCAGTGGACATACTTGTAGTTCTTCCACTTCCGCAGGTGCTAGTTTTTGCTGTAGTTCCCAATCTAAATGACTCCAGAATCTACAGTGCTGCAAAGTCCTTGAACTTTTTATTTATAATTCAGTACGTGCTGAGGGTTCTTCGAACCTGCTCCTTGTTAACACTCATTATAAGGGCTTCTGGCATCCTTGCTGAATCTGCATGGGCTAATCTCTCTTTCTTTATGCTGGCGAGTCATGTCCTTGGAGCCTTTTGGTATTTGTTTTCTATAGAAAGGAAAGCAACTTGCTGGCTGGAAGCATGTAAACATCTCGGTGTTAATTGTTCTTTGTACTGTGATGACACCTTAAGATCTAGCACACTTCTGAATGATTCTTGCTCTACAAATTCTACACTTTTTGATTTTGGAATATACTCTGATGCCCTTGAATCTGGAGTTGTTGATTCAACTCATTTTTTACAAAGGATTTCTTACGGCTTTTGGTGGGGACTGCAAAATCTCAGTGTTTCGGATAATCACATTTTAGAATTTGATGAACTATCTTCTGTTGTTAAAAAGACATATTTGCTATCAAAAGTTGCAAGATCAGAGGAAATGAGAGCCAAGCAACAAGAAATAGCAGTGTGGATGTCTTTCCATTCACTTCCGCGGAATCTTAAAACTCGAATTTGGCAGTCCCTGAAATACAAGCTCAAGGAAAACACGGGGATTGATGTGGAAACTTTTCTTCACCTTCTTCCTCGTGAACTCAGTAAGGATATAAAGCGACATATTTGCTATAGTCCACTCAGAGAAGTGCCATTACTTCGAAATAAGAATGAGGAACTGTTGGACGCAATCTGTGAACATCTGAAGCCAGTGCTTTACAAAAAGAATAGCATCATCTTTCAAGAGGGAGAGCCACTGGCTGAGATGCTGTTTATCACCCGTGGCAAGATAGTGACTTACACCACCTCTGCCATGGTAAGATTTCTTGGAAATGGTGACTGCTATGGTGAAGAACTTCTTGATTGGATGCTGCGTTCTTACGGTAAAGAGAATTGGGAGCTGAGTTCTACCAACATATCCAACTTTCCCTTGTCAAGCAAAACTGTCAAAGCTCAAACTGAAGTTGAAGTCTTTGTTCTAAGGGTCCATCAGTTAAAGCTTATATTGAAGAAATTCTGGTTCTGTTTTAGTAAGGAGATAAGTCTACAGCCATGGGCTGCATATACTTTGCAAGTAGCATGGCGCCGCTACAGAAGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

546

Amino Acids

62.8

Weight (kDa)

9.05

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 418 - 466 8.3e-06 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 506, 560
AarI CACCTGC 1 cut(s) 415
Acc36I ACCTGC 2 cut(s) 415, 545
AccB1I GGYRCC 1 cut(s) 1620
AccI GTMKAC 1 cut(s) 1582
AccII CGCG 2 cut(s) 215, 1039
AciI CCGC 4 cut(s) 422, 1037, 1039, 1624
AclWI GGATC 3 cut(s) 221, 234, 312
AcsI RAATTY 4 cut(s) 775, 917, 1056, 1550
AcuI CTGAAG 3 cut(s) 39, 1262, 1518
AcyI GRCGYC 1 cut(s) 1621
AfaI GTAC 3 cut(s) 127, 515, 722
AflII CTTAAG 1 cut(s) 736
AgsI TTSAA 8 cut(s) 85, 113, 493, 818, 837, 1280, 1505, 1546
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AloI GAACNNNNNNTCC 2 cut(s) 1396, 1428
AluBI AGCT 8 cut(s) 169, 175, 305, 1081, 1438, 1490, 1537, 1638
AluI AGCT 8 cut(s) 169, 175, 305, 1081, 1438, 1490, 1537, 1638
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 3 cut(s) 221, 234, 312
AlwNI CAGNNNCTG 1 cut(s) 1069
ApeKI GCWGC 3 cut(s) 479, 1408, 1595
ApoI RAATTY 4 cut(s) 775, 917, 1056, 1550
Asp700I GAANNNNTTC 3 cut(s) 1113, 1392, 1550
AspLEI GCGC 3 cut(s) 215, 217, 1623
AspS9I GGNCC 2 cut(s) 98, 1522
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 5 cut(s) 32, 1112, 1306, 1385, 1397
AsuII TTCGAA 2 cut(s) 532, 1198
AvaII GGWCC 2 cut(s) 98, 1522
BaeGI GKGCMC 1 cut(s) 81
BamHI GGATCC 1 cut(s) 226
BanI GGYRCC 1 cut(s) 1620
BarI GAAGNNNNNNTAC 2 cut(s) 1176, 1208
BauI CACGAG 1 cut(s) 1131
BbvCI CCTCAGC 1 cut(s) 521
BbvI GCAGC 3 cut(s) 466, 1395, 1582
BccI CCATC 1 cut(s) 1533
BceAI ACGGC 1 cut(s) 880
BcgI CGANNNNNNTGC 2 cut(s) 1146, 1180
BciVI GTATCC 1 cut(s) 3
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 4 cut(s) 102, 369, 431, 744
BfmI CTRYAG 6 cut(s) 441, 472, 657, 1168, 1583, 1627
BfoI RGCGCY 1 cut(s) 1624
BfrI CTTAAG 1 cut(s) 736
BfuAI ACCTGC 2 cut(s) 415, 545
BfuI GTATCC 1 cut(s) 3
BglII AGATCT 2 cut(s) 104, 740
BisI GCNGC 4 cut(s) 480, 1409, 1596, 1624
BlsI GCNGC 4 cut(s) 481, 1410, 1597, 1625
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 2 cut(s) 98, 1522
BmgT120I GGNCC 2 cut(s) 98, 1522
BmiI GGNNCC 5 cut(s) 99, 228, 637, 1523, 1622
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 6 cut(s) 355, 582, 799, 1278, 1293, 1395
BpmI CTGGAG 2 cut(s) 448, 843
Bpu10I CCTNAGC 1 cut(s) 521
Bpu14I TTCGAA 2 cut(s) 532, 1198
BpuEI CTTGAG 1 cut(s) 1067
BpuMI CCSGG 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 517
BsaBI GATNNNNATC 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 1621
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 5 cut(s) 631, 1037, 1318, 1350, 1589
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bse1I ACTGG 3 cut(s) 228, 1247, 1299
Bse8I GATNNNNATC 1 cut(s) 139
BseDI CCNNGG 5 cut(s) 631, 1037, 1318, 1350, 1589
BseGI GGATG 3 cut(s) 573, 1023, 1410
BseJI GATNNNNATC 1 cut(s) 139
BseMII CTCAG 7 cut(s) 337, 512, 904, 1153, 1192, 1290, 1430
BseNI ACTGG 3 cut(s) 228, 1247, 1299
BsePI GCGCGC 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 81
BseXI GCAGC 3 cut(s) 466, 1395, 1582
Bsh1236I CGCG 2 cut(s) 215, 1039
BshNI GGYRCC 1 cut(s) 1620
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 3 cut(s) 111, 891, 1051
BsmAI GTCTC 1 cut(s) 210
BsmFI GGGAC 3 cut(s) 111, 891, 1051
Bso31I GGTCTC 1 cut(s) 210
Bsp119I TTCGAA 2 cut(s) 532, 1198
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 5 cut(s) 104, 226, 317, 740, 974
Bsp19I CCATGG 2 cut(s) 1350, 1589
BspACI CCGC 4 cut(s) 422, 1037, 1039, 1624
BspCNI CTCAG 7 cut(s) 336, 513, 903, 1152, 1191, 1291, 1431
BspFNI CGCG 2 cut(s) 215, 1039
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 5 cut(s) 99, 228, 637, 1523, 1622
BspMI ACCTGC 2 cut(s) 415, 545
BspPI GGATC 3 cut(s) 221, 234, 312
BspT104I TTCGAA 2 cut(s) 532, 1198
BspT107I GGYRCC 1 cut(s) 1620
BspTI CTTAAG 1 cut(s) 736
BspTNI GGTCTC 1 cut(s) 210
BsrI ACTGG 3 cut(s) 228, 1247, 1299
BssECI CCNNGG 5 cut(s) 631, 1037, 1318, 1350, 1589
BssHII GCGCGC 1 cut(s) 213
BssMI GATC 5 cut(s) 104, 226, 317, 740, 974
BssNI GRCGYC 1 cut(s) 1621
BssSI CACGAG 1 cut(s) 1131
BssT1I CCWWGG 3 cut(s) 631, 1350, 1589
Bst2BI CACGAG 1 cut(s) 1131
Bst4CI ACNGT 6 cut(s) 310, 476, 725, 1218, 1421, 1483
BstACI GRCGYC 1 cut(s) 1621
BstAFI CTTAAG 1 cut(s) 736
BstBAI YACGTR 1 cut(s) 517
BstBI TTCGAA 2 cut(s) 532, 1198
BstC8I GCNNGC 3 cut(s) 215, 618, 680
BstDEI CTNAG 9 cut(s) 209, 323, 521, 890, 1139, 1178, 1299, 1439, 1517
BstDSI CCRYGG 4 cut(s) 1037, 1318, 1350, 1589
BstF5I GGATG 3 cut(s) 573, 1023, 1410
BstFNI CGCG 2 cut(s) 215, 1039
BstH2I RGCGCY 1 cut(s) 1624
BstHHI GCGC 3 cut(s) 215, 217, 1623
BstKTI GATC 5 cut(s) 107, 229, 320, 743, 977
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 5 cut(s) 104, 226, 317, 740, 974
BstMWI GCNNNNNNNGC 1 cut(s) 587
BstNSI RCATGY 1 cut(s) 693
BstSCI CCNGG 1 cut(s) 40
BstSFI CTRYAG 6 cut(s) 441, 472, 657, 1168, 1583, 1627
BstSLI GKGCMC 1 cut(s) 81
BstUI CGCG 2 cut(s) 215, 1039
BstV1I GCAGC 3 cut(s) 466, 1395, 1582
BstX2I RGATCY 3 cut(s) 104, 226, 740
BstYI RGATCY 3 cut(s) 104, 226, 740
BsuI GTATCC 1 cut(s) 3
BtgI CCRYGG 4 cut(s) 1037, 1318, 1350, 1589
BtsCI GGATG 3 cut(s) 573, 1023, 1410
BtsI GCAGTG 2 cut(s) 399, 1017
BtsIMutI CAGTG 7 cut(s) 331, 399, 481, 898, 1017, 1254, 1292
BveI ACCTGC 2 cut(s) 415, 545
Cac8I GCNNGC 3 cut(s) 215, 618, 680
CaiI CAGNNNCTG 1 cut(s) 1069
CciI TCATGA 1 cut(s) 252
CfoI GCGC 3 cut(s) 215, 217, 1623
Cfr13I GGNCC 2 cut(s) 98, 1522
Cfr42I CCGCGG 1 cut(s) 1040
CseI GACGC 1 cut(s) 1232
Csp6I GTAC 3 cut(s) 126, 514, 721
CviAII CATG 7 cut(s) 253, 591, 626, 690, 1351, 1590, 1617
CviQI GTAC 3 cut(s) 126, 514, 721
DdeI CTNAG 9 cut(s) 209, 323, 521, 890, 1139, 1178, 1299, 1439, 1517
DinI GGCGCC 1 cut(s) 1622
DpnI GATC 5 cut(s) 106, 228, 319, 742, 976
DpnII GATC 5 cut(s) 104, 226, 317, 740, 974
Eco130I CCWWGG 3 cut(s) 631, 1350, 1589
Eco31I GGTCTC 1 cut(s) 210
Eco47I GGWCC 2 cut(s) 98, 1522
Eco57I CTGAAG 3 cut(s) 39, 1262, 1518
EcoO109I RGGNCCY 1 cut(s) 98
EcoT14I CCWWGG 3 cut(s) 631, 1350, 1589
EgeI GGCGCC 1 cut(s) 1622
EheI GGCGCC 1 cut(s) 1622
ErhI CCWWGG 3 cut(s) 631, 1350, 1589
FaeI CATG 7 cut(s) 256, 594, 629, 693, 1354, 1593, 1620
FalI AAGNNNNNCTT 6 cut(s) 37, 69, 844, 876, 1380, 1412
FaqI GGGAC 3 cut(s) 111, 891, 1051
FatI CATG 7 cut(s) 252, 590, 625, 689, 1350, 1589, 1616
FblI GTMKAC 1 cut(s) 1582
Fnu4HI GCNGC 4 cut(s) 480, 1409, 1596, 1624
FokI GGATG 3 cut(s) 560, 1030, 1417
Fsp4HI GCNGC 4 cut(s) 480, 1409, 1596, 1624
FspBI CTAG 4 cut(s) 102, 369, 431, 744
GlaI GCGC 3 cut(s) 214, 216, 1622
GluI GCNGC 4 cut(s) 480, 1409, 1596, 1624
GsuI CTGGAG 2 cut(s) 448, 843
HaeII RGCGCY 1 cut(s) 1624
HapII CCGG 1 cut(s) 42
HgaI GACGC 1 cut(s) 1232
HhaI GCGC 3 cut(s) 215, 217, 1623
Hin1I GRCGYC 1 cut(s) 1621
Hin1II CATG 7 cut(s) 256, 594, 629, 693, 1354, 1593, 1620
Hin6I GCGC 3 cut(s) 213, 215, 1621
HinP1I GCGC 3 cut(s) 213, 215, 1621
HincII GTYRAC 2 cut(s) 163, 549
HindII GTYRAC 2 cut(s) 163, 549
HindIII AAGCTT 1 cut(s) 1535
HinfI GANTC 9 cut(s) 62, 461, 468, 584, 622, 761, 818, 833, 1042
HpaI GTTAAC 1 cut(s) 549
HpaII CCGG 1 cut(s) 42
HphI GGTGA 5 cut(s) 32, 1112, 1306, 1385, 1397
Hpy166II GTNNAC 8 cut(s) 163, 397, 549, 695, 1136, 1175, 1235, 1583
Hpy188I TCNGA 6 cut(s) 756, 808, 901, 979, 1181, 1242
Hpy188III TCNNGA 7 cut(s) 181, 253, 465, 822, 1133, 1280, 1397
Hpy8I GTNNAC 8 cut(s) 163, 397, 549, 695, 1136, 1175, 1235, 1583
HpyAV CCTTC 2 cut(s) 1133, 1626
HpyCH4III ACNGT 6 cut(s) 310, 476, 725, 1218, 1421, 1483
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 8 cut(s) 273, 392, 482, 590, 883, 971, 1598, 1609
HpyF10VI GCNNNNNNNGC 1 cut(s) 587
HpyF3I CTNAG 9 cut(s) 209, 323, 521, 890, 1139, 1178, 1299, 1439, 1517
HpySE526I ACGT 1 cut(s) 516
Hsp92I GRCGYC 1 cut(s) 1621
Hsp92II CATG 7 cut(s) 256, 594, 629, 693, 1354, 1593, 1620
HspAI GCGC 3 cut(s) 213, 215, 1621
KasI GGCGCC 1 cut(s) 1620
KspAI GTTAAC 1 cut(s) 549
KspI CCGCGG 1 cut(s) 1040
Kzo9I GATC 5 cut(s) 104, 226, 317, 740, 974
LmnI GCTCC 5 cut(s) 43, 545, 635, 1435, 1635
Lsp1109I GCAGC 3 cut(s) 466, 1395, 1582
LweI GCATC 6 cut(s) 355, 582, 799, 1278, 1293, 1395
MaeI CTAG 4 cut(s) 102, 369, 431, 744
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 4 cut(s) 326, 376, 1330, 1373
MalI GATC 5 cut(s) 106, 228, 319, 742, 976
MboI GATC 5 cut(s) 104, 226, 317, 740, 974
MflI RGATCY 3 cut(s) 104, 226, 740
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 7 cut(s) 507, 709, 775, 917, 1056, 1429, 1550
Mly113I GGCGCC 1 cut(s) 1621
MlyI GAGTC 2 cut(s) 455, 631
MmeI TCCRAC 2 cut(s) 1200, 1482
MnlI CCTC 8 cut(s) 332, 341, 516, 973, 1140, 1204, 1276, 1354
MroXI GAANNNNTTC 3 cut(s) 1113, 1392, 1550
MseI TTAA 7 cut(s) 137, 548, 708, 737, 942, 1047, 1532
MslI CAYNNNNRTG 2 cut(s) 363, 702
MspA1I CMGCKG 1 cut(s) 1039
MspCI CTTAAG 1 cut(s) 736
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 1 cut(s) 42
MvnI CGCG 2 cut(s) 215, 1039
MwoI GCNNNNNNNGC 1 cut(s) 587
NarI GGCGCC 1 cut(s) 1621
NciI CCSGG 1 cut(s) 42
NcoI CCATGG 2 cut(s) 1350, 1589
NdeII GATC 5 cut(s) 104, 226, 317, 740, 974
NlaIII CATG 7 cut(s) 256, 594, 629, 693, 1354, 1593, 1620
NlaIV GGNNCC 5 cut(s) 99, 228, 637, 1523, 1622
NmuCI GTSAC 3 cut(s) 326, 1330, 1373
NspI RCATGY 1 cut(s) 693
NspV TTCGAA 2 cut(s) 532, 1198
PagI TCATGA 1 cut(s) 252
PaqCI CACCTGC 1 cut(s) 415
PauI GCGCGC 1 cut(s) 213
PdmI GAANNNNTTC 3 cut(s) 1113, 1392, 1550
PfeI GAWTC 7 cut(s) 62, 468, 584, 761, 818, 833, 1042
PfoI TCCNGGA 1 cut(s) 40
PkrI GCNGC 4 cut(s) 481, 1410, 1597, 1625
PleI GAGTC 2 cut(s) 455, 630
PluTI GGCGCC 1 cut(s) 1624
PpsI GAGTC 2 cut(s) 455, 630
Ppu21I YACGTR 1 cut(s) 517
PpuMI RGGWCCY 1 cut(s) 98
PsiI TTATAA 2 cut(s) 506, 560
Psp5II RGGWCCY 1 cut(s) 98
PspN4I GGNNCC 5 cut(s) 99, 228, 637, 1523, 1622
PspPI GGNCC 2 cut(s) 98, 1522
PspPPI RGGWCCY 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 1069
PsuI RGATCY 3 cut(s) 104, 226, 740
PteI GCGCGC 1 cut(s) 213
RsaI GTAC 3 cut(s) 127, 515, 722
RsaNI GTAC 3 cut(s) 126, 514, 721
RseI CAYNNNNRTG 2 cut(s) 363, 702
SacII CCGCGG 1 cut(s) 1040
SaqAI TTAA 7 cut(s) 137, 548, 708, 737, 942, 1047, 1532
SatI GCNGC 4 cut(s) 480, 1409, 1596, 1624
Sau3AI GATC 5 cut(s) 104, 226, 317, 740, 974
Sau96I GGNCC 2 cut(s) 98, 1522
SchI GAGTC 2 cut(s) 455, 631
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 1 cut(s) 81
SfaNI GCATC 6 cut(s) 355, 582, 799, 1278, 1293, 1395
SfcI CTRYAG 6 cut(s) 441, 472, 657, 1168, 1583, 1627
SfoI GGCGCC 1 cut(s) 1622
Sfr303I CCGCGG 1 cut(s) 1040
SfuI TTCGAA 2 cut(s) 532, 1198
SgrBI CCGCGG 1 cut(s) 1040
SinI GGWCC 2 cut(s) 98, 1522
SmiMI CAYNNNNRTG 2 cut(s) 363, 702
SmlI CTYRAG 2 cut(s) 736, 1082
SmoI CTYRAG 2 cut(s) 736, 1082
Sse9I AATT 7 cut(s) 507, 709, 775, 917, 1056, 1429, 1550
SsiI CCGC 4 cut(s) 422, 1037, 1039, 1624
SspDI GGCGCC 1 cut(s) 1620
SspI AATATT 1 cut(s) 291
SspMI CTAG 4 cut(s) 102, 369, 431, 744
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 3 cut(s) 631, 1350, 1589
TaaI ACNGT 6 cut(s) 310, 476, 725, 1218, 1421, 1483
TaiI ACGT 1 cut(s) 519
TaqI TCGA 4 cut(s) 363, 532, 1054, 1198
TasI AATT 7 cut(s) 507, 709, 775, 917, 1056, 1429, 1550
TatI WGTACW 1 cut(s) 720
TauI GCSGC 1 cut(s) 1626
TfiI GAWTC 7 cut(s) 62, 468, 584, 761, 818, 833, 1042
Tru1I TTAA 7 cut(s) 137, 548, 708, 737, 942, 1047, 1532
Tru9I TTAA 7 cut(s) 137, 548, 708, 737, 942, 1047, 1532
TscAI CASTG 7 cut(s) 331, 399, 481, 898, 1017, 1254, 1299
TseFI GTSAC 3 cut(s) 326, 1330, 1373
TseI GCWGC 3 cut(s) 479, 1408, 1595
Tsp45I GTSAC 3 cut(s) 326, 1330, 1373
TspDTI ATGAA 2 cut(s) 335, 939
TspRI CASTG 7 cut(s) 331, 399, 481, 898, 1017, 1254, 1299
Vha464I CTTAAG 1 cut(s) 736
VpaK11BI GGWCC 2 cut(s) 98, 1522
XapI RAATTY 4 cut(s) 775, 917, 1056, 1550
XceI RCATGY 1 cut(s) 693
XcmI CCANNNNNNNNNTGG 1 cut(s) 1348
XmiI GTMKAC 1 cut(s) 1582
XmnI GAANNNNTTC 3 cut(s) 1113, 1392, 1550
XspI CTAG 4 cut(s) 102, 369, 431, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.