MD02G1303700.v1.1

3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000889
Physical Location & Seq
Reverse (-)
35737057 .. 35741257
4201 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1303700.v1.1.491

Sequence Viewer

Length: 3822 bp
ATGGCGTCTTCGACTTCTTCTCCTGTGCAACCTGAAGTTGTTGCTACTCCTCCTCCTGCACCTTCTTCCCCCAATTTTGCTACGATGAACCCTAATTCTTCCTCTTCTTCAATTTCTTCTATAACTGTTCAGAATATCAGCTGTATGGTTCCTACGAAGCTCAAACGTGACAATTATCTCGTCTGGAAAGCTCTATTCGCTCCAATTTTCCGACGATACAAACTCACCGGCATTGTTGATGGCTCGAAAATTTGCCCACCGCCGTTTGTTCTTGATGCATTTGGTTGCAGCATTGGAGTTCCAAATCCTGACTTCGAGCTGTGGTATGAAAAAGATCAGAATATCCTGATCTGGTTGAACTCCACTCTTTCTAAGGATATTGTTCCGTTTACGGTTGGTGTCACCTCGTCTCGCGATCTCTGGTTAAACCTTGAGCATCGATTTGGTGGCGTGTCTGCCGCTCACATTCATCAGCTTCGTTCTCGCCTTCACTCTATTCATAAAGGAGATCTCTCTATCTCCGATTATCTGCAGCGCATCAAAGGTCTTGCTGATTCTTTAATGGCTGCTGGAGCTTCTGTTTCTGATCCAGATTTGATTGCCAAATGTGTGGCCTCCTCCGCTTCTGAGCCTTTTCAGGCATTTACTGCGCAGTTTCAACCTCCTCTTCTTCCTACACCATCTCAGTTCTATAATTTTCCTCAAGCATTTGCTGCTCAACAGTCATCTTACAGTGCGCCTTCCAAGTTTCACAACAATAATCGAGATCGAGGTTTCTCTCAGCGTAATCATCGTGGTAATTTTTCTAAGAACTTCAACAATCGTGGTCAATTCAACAATTCTGGTGGGAATCATGGCAATTACAGAAACACCTCTGGAAATCGCAATCATTTTGGTGGTAATCGTGGTCAATTCCACAATTCTGGTGGTCGCACTTCCTGCCAAATATGTAATTCGTTTGATCACAAAGCTCTGGATTGCTATGCACGGATGAATCATGCTTTTGCAGGCAAAATCCCTCCTGCACAGCTCGCTGCTATGTGTGTTCACACCAACTCTAAGCCATCATCTCCCACTTGGTTGATGGACTCGGGTGCCACCTCTCATATTGTTAATGATATTATGCGATTCAGTCTCCCCGTCCGTATTCTGGCGATGACAAGGTGTATGTTGGTGATGGTCAAGGATCGTTCTTCGGGGAGGATGCTTTTTCGAGGGCCAGTTAAAGAAGGCTTCTATCCATTTCACGGTCTCTCTTCGGTTTCTTCTTCACATACTGCTCTTCTCAGCACTAAAGCCTCTTTGAAGACATGGCATCACAGACTTGGTCATCCTTCCTCTGCTATGTTTAGGAAAGTTTTCAATAAGCATTGTCTTGATCACATTGGTGATAAGTCTGTATCTTTCTTTTGCTCTGATTGTGCCATCGGCAAGAACCACAAGCTGCCCTTTTTCTCTAGTACTAGTTCTGCTTCAACTAGTTTAAAGTTGCTCCATTGTGATGTTTGGGGTCCCTCTCCAGTCCTTTCTGTTACTGGTTACAGATTCTACCTGTTGATTGTGGATGAGTTTACTAAATACACATGGATGTTTCCCATGAAAGCTAAGTCTGAGGTTTTTTCTGTATTTGTCCACTTCAAAGCTTATGTAGAGAACATGATTGGCAATAAAATAAAGATTTTGAGATCAGATTCAGGGGGTGAGTTCACCAGTTCCGTGTTCAATTCTTTTCTCTCTCGCAATGGTATTTCTCATCAATATAGCTGTCCTCACACTCCGGAACAAAATGGATGTGTTGAACGGAAGCATCGCCATCTTACCGAGACTGCACGAACTCTTTTGGTTTCTTCTCGGGTTCCACACAAGTTTTGGGTTGAAGCTTTTGCAACTGCTTTTTATCTCATTAACCGACTGCCTATTTCTCGGTTGGATACATCTCCTTGGGAACTTCTCTTTCGACGTTCTCCAGATTATTCCAAGTTAAAGGTCTTTGGATGCAGCTGCTACCTTTGGCTCAAACCCTATGTTTCCTCGAACTTGGACGACAAAAGCAAACACTGTGTCTTCCTTAGTTATAGCCTTCAGCACAAAGGCTATCGTTGCCTAGATCTCCAGTCTGAGCGCATTTATATTTCAAGGCATGTTCTGTTTAATGAAGAGCATTTTCCGTTTCATGATGCCCATCACCTTTCTTCTTCCTCACTCCCACAGTCCACTGCCTCTACATCATATCCACTCTTCACTCTCCCTTTCCCTTCCTTTCAACATACTCCTGCCTCCTCACCAATCTCTTCATCTCCGCTAGTTCCCCCTGCACTATCTCAGCCATTATCTTCTTCTTCGGTCCCGTCATCTTCATCTTCTTCTCCATCTAGTGCTTCCTTACCTCTGCCATCCCATCCATCCTCTTCCATTCAACCCTCTATTCCCTCTAATTCTCATCCTATGATCACACGGTCTAAAGCCGGCATTTTCAAACCCAAAGCTCTATCTGCCACTAAACATCCCATCAATATTGATACTTTTGTGCCTACCACTTATCTGCAAGCATCTAAGCATGCTCATTGGGTTCAGGCCACATCAGAAGAATTTGAGGCTCTTCGCCACACTGGTACTTGGACATTGGTACCTTCTTCTCCTCACCACAATCTTGTTGGCTGCAAATGGGTGTTTCGGATTAAAAGGAAACCTGATGGCTCGATTGATCGTTACAAGGCTCGCCTTGTAGCCAAAGGATTTCACCAGCAGGCCGATATTGATTTTACAGAAACTTTCAGCCCTGTTGCCAAACCAGTAACCATTCGCATTCTATTATCACTTGCTGTCCACAATGACTGCCTCCTGGTTTTGTTGATGCTGCTCAGCCTTCTTTTGTCTGTAAGCTCAATCGCTCTCTTTATTCACAAGCTCAGTACCTTGTTTCCTGTCAAAGACTTAGGTTCTCTCCACTATTTCCTTGGTCTTGAAGTCCATCGATCCTCTGATGGTATCTTCATGTCTCAAGGCAAATATGCTATGGATCTTCTTATTAAGACAAAAATGGAAGGCTGCAAGCCTTGTGCCACTCCTCTTGGTACTGCCAAATTAGATCACATTGGAACTCTGTTGCCTGATCCAAAGGCGTATCGGTCTATAGTTGGTGCTCTCCAATATCTCACATGGACTAGGCCAGATTTGTCATTTGCTGTGAACCAAGTCTGTCAGTTTCTTCATTGCCCTCGTGACACTCATTTTCAGGCTGTTAAACGCATTCTCCGGTTTCTCAAGGGTTCGGTTGTTCAAGGCCTCTGGTTCAAAAAGGGATCTCTGCAGCTCACAGCTTTCTCGGATGCTGATTGGGCTGGTTGTGTTTTTTATAGACGTTCTACATCTGGTTATTGTGTTTACCTTGGTCCCAATCTTATCAGTTGGAGTGCTAAAAAGCAGGCCACTGTTGCCCGCTCTTCAACCGAGGCCGAGTATCGCTCTCTTGCTCTTACTGCTGCCGAACTTACATGGATCTGCAAGATTTTCAAAGACATTACTTTCCCTCTTTCTCATACTCCAACACTGTGGTGTGACAATGTTTCTGCAATCTCTTTGGCTTCCAATCTTGTGTTTCATGCGCGCACAAAACATGTGGAGATCGATTATCACTACATTAGAGAGCTTGTTCTTGCTGGTTTAGTCAAAGTGTTGTATGTTTCCACTGAACATCAGATTGCAGACATCCATACAAAGTCCCTTTCCAAGGCCCGGTTTCACTTTCTTCAGTCCAAGCTTTCTCTGGGTTCTCCTCCGCTTAGCTTGAGGGGGTGTAAAGGGATAAGTAGTCATAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000137 GO:0000139 GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0005795 GO:0006029 GO:0006082 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006862 GO:0008150 GO:0008152 GO:0008509 GO:0008514 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0015012 GO:0015075 GO:0015215 GO:0015216 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015868 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0022857 GO:0030166 GO:0030173 GO:0030176 GO:0030201 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0031985 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034035 GO:0034036 GO:0034220 GO:0034641 GO:0034645 GO:0034654 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046390 GO:0046483 GO:0046963 GO:0046964 GO:0050427 GO:0050428 GO:0051179 GO:0051234 GO:0051503 GO:0055085 GO:0055086 GO:0071702 GO:0071704 GO:0071705 GO:0072348 GO:0072521 GO:0072522 GO:0072530 GO:0090407 GO:0098588 GO:0098656 GO:0098791 GO:0098827 GO:1901135 GO:1901137 GO:1901264 GO:1901293 GO:1901360 GO:1901362 GO:1901505 GO:1901564 GO:1901566 GO:1901576 GO:1901679 GO:1901682 GO:1902559
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UAA

Protein Analysis

1274

Amino Acids

141.66

Weight (kDa)

9.57

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 106 - 218 8.3e-07 gag-polypeptide of LTR copia-type
gag_pre-integrs PF13976 413 - 478 2e-10 GAG-pre-integrase domain
rve PF00665 497 - 593 5e-09 Integrase core domain
SH3_retrovirus PF25597 666 - 726 5.9e-19 Retroviral polymerase SH3-like domain
RVT_2 PF07727 872 - 947 1.2e-15 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 962 - 1024 1.6e-06 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 651
Acc65I GGTACC 1 cut(s) 2626
AccB1I GGYRCC 2 cut(s) 1094, 2626
AccBSI CCGCTC 2 cut(s) 461, 3441
AccII CGCG 2 cut(s) 414, 3607
AccIII TCCGGA 1 cut(s) 1777
AciI CCGC 6 cut(s) 260, 459, 621, 2300, 3439, 3779
AclWI GGATC 7 cut(s) 581, 1194, 2970, 3027, 3107, 3310, 3506
AcsI RAATTY 2 cut(s) 249, 2588
AcuI CTGAAG 3 cut(s) 54, 2066, 3734
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 1462, 2614, 2628, 2914, 3076
AfiI CCNNNNNNNGG 4 cut(s) 1150, 1247, 3730, 3735
AflIII ACRYGT 1 cut(s) 3616
AhlI ACTAGT 2 cut(s) 1463, 1478
AjnI CCWGG 1 cut(s) 2841
AleI CACNNNNGTG 2 cut(s) 1386, 3553
Alw21I GWGCWC 1 cut(s) 3145
Alw26I GTCTC 5 cut(s) 414, 1139, 1256, 1817, 3003
AlwI GGATC 7 cut(s) 581, 1194, 2970, 3027, 3107, 3310, 3506
Ama87I CYCGRG 2 cut(s) 1090, 1851
Aor13HI TCCGGA 1 cut(s) 1777
AoxI GGCC 9 cut(s) 612, 1217, 2574, 2748, 3167, 3283, 3426, 3453, 3732
ApoI RAATTY 2 cut(s) 249, 2588
ArsI GACNNNNNNTTYG 2 cut(s) 2046, 2078
Asp700I GAANNNNTTC 1 cut(s) 1358
Asp718I GGTACC 1 cut(s) 2626
AspLEI GCGC 6 cut(s) 537, 652, 739, 2124, 3607, 3609
AspS9I GGNCC 5 cut(s) 1217, 1511, 2344, 3392, 3733
AsuC2I CCSGG 1 cut(s) 3736
AvaI CYCGRG 2 cut(s) 1090, 1851
AvaII GGWCC 3 cut(s) 1511, 2344, 3392
BaeI ACNNNNGTAYC 4 cut(s) 2511, 2544, 2610, 2643
BanI GGYRCC 2 cut(s) 1094, 2626
BauI CACGAG 1 cut(s) 3219
BbsI GAAGAC 2 cut(s) 1313, 2056
Bbv12I GWGCWC 1 cut(s) 3145
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 2843
BciVI GTATCC 1 cut(s) 1924
BclI TGATCA 3 cut(s) 961, 1378, 2448
BcnI CCSGG 1 cut(s) 3736
BcoDI GTCTC 5 cut(s) 414, 1139, 1256, 1817, 3003
BcuI ACTAGT 2 cut(s) 1463, 1478
BfaI CTAG 7 cut(s) 1458, 1464, 1479, 2105, 2303, 2373, 3165
BfmI CTRYAG 3 cut(s) 530, 3132, 3308
BfuI GTATCC 1 cut(s) 1924
BglII AGATCT 2 cut(s) 508, 2107
BlpI GCTNAGC 2 cut(s) 2861, 3782
BmcAI AGTACT 1 cut(s) 1462
Bme1390I CCNGG 2 cut(s) 2843, 3736
Bme18I GGWCC 3 cut(s) 1511, 2344, 3392
BmeT110I CYCGRG 2 cut(s) 1090, 1851
BmgT120I GGNCC 5 cut(s) 1217, 1511, 2344, 3392, 3733
BmiI GGNNCC 8 cut(s) 150, 1096, 1512, 1513, 1857, 2346, 2628, 3394
BmrFI CCNGG 2 cut(s) 2843, 3736
BpiI GAAGAC 2 cut(s) 1313, 2056
BplI GAGNNNNNCTC 2 cut(s) 3449, 3481
BpmI CTGGAG 4 cut(s) 591, 1503, 1950, 2096
Bpu1102I GCTNAGC 2 cut(s) 2861, 3782
BpuEI CTTGAG 5 cut(s) 452, 687, 2985, 3248, 3808
BpuMI CCSGG 1 cut(s) 3736
Bsa29I ATCGAT 3 cut(s) 439, 2974, 3627
BsaBI GATNNNNATC 1 cut(s) 2181
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1256
BsaJI CCNNGG 5 cut(s) 1940, 2956, 3388, 3450, 3729
BsaWI WCCGGW 2 cut(s) 1777, 3255
BsaXI ACNNNNNCTCC 7 cut(s) 34, 37, 67, 564, 594, 3237, 3267
Bsc4I CCNNNNNNNGG 4 cut(s) 1150, 1247, 3730, 3735
Bse118I RCCGGY 2 cut(s) 227, 2465
Bse1I ACTGG 7 cut(s) 1220, 1520, 1540, 1710, 2113, 2614, 2792
Bse3DI GCAATG 2 cut(s) 1747, 3211
Bse8I GATNNNNATC 1 cut(s) 2181
BseAI TCCGGA 1 cut(s) 1777
BseBI CCWGG 1 cut(s) 2843
BseCI ATCGAT 3 cut(s) 439, 2974, 3627
BseDI CCNNGG 5 cut(s) 1940, 2956, 3388, 3450, 3729
BseJI GATNNNNATC 1 cut(s) 2181
BseLI CCNNNNNNNGG 4 cut(s) 1150, 1247, 3730, 3735
BseMI GCAATG 2 cut(s) 1747, 3211
BseMII CTCAG 9 cut(s) 618, 698, 794, 1300, 1602, 2109, 2336, 2875, 2923
BseNI ACTGG 7 cut(s) 1220, 1520, 1540, 1710, 2113, 2614, 2792
BsePI GCGCGC 1 cut(s) 3605
BseRI GAGGAG 8 cut(s) 39, 42, 607, 654, 2269, 2628, 3057, 3765
BsgI GTGCAG 4 cut(s) 42, 1008, 1812, 2298
Bsh1236I CGCG 2 cut(s) 414, 3607
BshFI GGCC 9 cut(s) 614, 1219, 2576, 2750, 3169, 3285, 3428, 3455, 3734
BshNI GGYRCC 2 cut(s) 1094, 2626
BshVI ATCGAT 3 cut(s) 439, 2974, 3627
BsiHKAI GWGCWC 1 cut(s) 3145
BsiHKCI CYCGRG 2 cut(s) 1090, 1851
BsiSI CCGG 5 cut(s) 228, 1778, 2466, 3256, 3736
BslFI GGGAC 4 cut(s) 1497, 2330, 3378, 3706
BslI CCNNNNNNNGG 4 cut(s) 1150, 1247, 3730, 3735
BsmAI GTCTC 5 cut(s) 414, 1139, 1256, 1817, 3003
BsmBI CGTCTC 1 cut(s) 414
BsmFI GGGAC 4 cut(s) 1497, 2330, 3378, 3706
BsmI GAATGC 2 cut(s) 2805, 3249
BsnI GGCC 9 cut(s) 614, 1219, 2576, 2750, 3169, 3285, 3428, 3455, 3734
Bso31I GGTCTC 1 cut(s) 1256
BsoBI CYCGRG 2 cut(s) 1090, 1851
Bsp1286I GDGCHC 1 cut(s) 3145
Bsp13I TCCGGA 1 cut(s) 1777
Bsp1720I GCTNAGC 2 cut(s) 2861, 3782
Bsp68I TCGCGA 1 cut(s) 414
BspACI CCGC 6 cut(s) 260, 459, 621, 2300, 3439, 3779
BspANI GGCC 9 cut(s) 614, 1219, 2576, 2750, 3169, 3285, 3428, 3455, 3734
BspCNI CTCAG 9 cut(s) 619, 697, 793, 1299, 1603, 2110, 2335, 2874, 2922
BspDI ATCGAT 3 cut(s) 439, 2974, 3627
BspEI TCCGGA 1 cut(s) 1777
BspFNI CGCG 2 cut(s) 414, 3607
BspHI TCATGA 1 cut(s) 2173
BspLI GGNNCC 8 cut(s) 150, 1096, 1512, 1513, 1857, 2346, 2628, 3394
BspMAI CTGCAG 2 cut(s) 534, 3312
BspPI GGATC 7 cut(s) 581, 1194, 2970, 3027, 3107, 3310, 3506
BspQI GCTCTTC 4 cut(s) 1287, 2151, 2604, 3448
BspT107I GGYRCC 2 cut(s) 1094, 2626
BspTNI GGTCTC 1 cut(s) 1256
BsrBI CCGCTC 2 cut(s) 461, 3441
BsrDI GCAATG 2 cut(s) 1747, 3211
BsrFI RCCGGY 2 cut(s) 227, 2465
BsrI ACTGG 7 cut(s) 1220, 1520, 1540, 1710, 2113, 2614, 2792
BssAI RCCGGY 2 cut(s) 227, 2465
BssECI CCNNGG 5 cut(s) 1940, 2956, 3388, 3450, 3729
BssHII GCGCGC 1 cut(s) 3605
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 3219
BssT1I CCWWGG 4 cut(s) 1940, 2956, 3388, 3729
Bst2BI CACGAG 1 cut(s) 3219
Bst2UI CCWGG 1 cut(s) 2843
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 3 cut(s) 647, 713, 939
BstENI CCTNNNNNAGG 1 cut(s) 3728
BstFNI CGCG 2 cut(s) 414, 3607
BstHHI GCGC 6 cut(s) 537, 652, 739, 2124, 3607, 3609
BstMAI GTCTC 5 cut(s) 414, 1139, 1256, 1817, 3003
BstNI CCWGG 1 cut(s) 2843
BstNSI RCATGY 3 cut(s) 2144, 2561, 3620
BstSCI CCNGG 2 cut(s) 2841, 3734
BstSFI CTRYAG 3 cut(s) 530, 3132, 3308
BstUI CGCG 2 cut(s) 414, 3607
BstV2I GAAGAC 2 cut(s) 1313, 2056
BstX2I RGATCY 5 cut(s) 508, 2107, 3019, 3302, 3498
BstXI CCANNNNNNTGG 3 cut(s) 610, 923, 3552
BstYI RGATCY 5 cut(s) 508, 2107, 3019, 3302, 3498
Bsu15I ATCGAT 3 cut(s) 439, 2974, 3627
BsuI GTATCC 1 cut(s) 1924
BsuRI GGCC 9 cut(s) 614, 1219, 2576, 2750, 3169, 3285, 3428, 3455, 3734
BsuTUI ATCGAT 3 cut(s) 439, 2974, 3627
BtgZI GCGATG 2 cut(s) 1169, 1793
BtsI GCAGTG 1 cut(s) 2214
BtsIMutI CAGTG 7 cut(s) 739, 2056, 2214, 2607, 3429, 3548, 3687
BtuMI TCGCGA 1 cut(s) 414
CciI TCATGA 1 cut(s) 2173
CfoI GCGC 6 cut(s) 537, 652, 739, 2124, 3607, 3609
Cfr10I RCCGGY 2 cut(s) 227, 2465
Cfr13I GGNCC 5 cut(s) 1217, 1511, 2344, 3392, 3733
ClaI ATCGAT 3 cut(s) 439, 2974, 3627
Csp6I GTAC 5 cut(s) 1461, 2613, 2627, 2913, 3075
CviQI GTAC 5 cut(s) 1461, 2613, 2627, 2913, 3075
DraI TTTAAA 1 cut(s) 1485
Eco130I CCWWGG 4 cut(s) 1940, 2956, 3388, 3729
Eco147I AGGCCT 1 cut(s) 3285
Eco31I GGTCTC 1 cut(s) 1256
Eco47I GGWCC 3 cut(s) 1511, 2344, 3392
Eco57I CTGAAG 3 cut(s) 54, 2066, 3734
Eco88I CYCGRG 2 cut(s) 1090, 1851
EcoNI CCTNNNNNAGG 1 cut(s) 3728
EcoO109I RGGNCCY 1 cut(s) 1511
EcoRII CCWGG 1 cut(s) 2841
EcoT14I CCWWGG 4 cut(s) 1940, 2956, 3388, 3729
EcoT22I ATGCAT 1 cut(s) 280
ErhI CCWWGG 4 cut(s) 1940, 2956, 3388, 3729
Esp3I CGTCTC 1 cut(s) 414
FalI AAGNNNNNCTT 2 cut(s) 1433, 1465
FaqI GGGAC 4 cut(s) 1497, 2330, 3378, 3706
FauI CCCGC 1 cut(s) 3446
FbaI TGATCA 3 cut(s) 961, 1378, 2448
FspBI CTAG 7 cut(s) 1458, 1464, 1479, 2105, 2303, 2373, 3165
FspI TGCGCA 1 cut(s) 651
GlaI GCGC 6 cut(s) 536, 651, 738, 2123, 3606, 3608
GsuI CTGGAG 4 cut(s) 591, 1503, 1950, 2096
HaeIII GGCC 9 cut(s) 614, 1219, 2576, 2750, 3169, 3285, 3428, 3455, 3734
HapII CCGG 5 cut(s) 228, 1778, 2466, 3256, 3736
HhaI GCGC 6 cut(s) 537, 652, 739, 2124, 3607, 3609
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 6 cut(s) 535, 650, 737, 2122, 3605, 3607
HinP1I GCGC 6 cut(s) 535, 650, 737, 2122, 3605, 3607
HindIII AAGCTT 3 cut(s) 1641, 1878, 3758
HinfI GANTC 7 cut(s) 554, 850, 994, 1088, 1128, 1545, 1691
HpaII CCGG 5 cut(s) 228, 1778, 2466, 3256, 3736
Hpy166II GTNNAC 9 cut(s) 390, 1048, 1572, 1633, 1707, 2214, 2827, 3190, 3385
Hpy8I GTNNAC 9 cut(s) 390, 1048, 1572, 1633, 1707, 2214, 2827, 3190, 3385
Hpy99I CGWCG 2 cut(s) 216, 1962
HpyCH4IV ACGT 3 cut(s) 166, 1960, 3361
HpySE526I ACGT 3 cut(s) 166, 1960, 3361
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 6 cut(s) 535, 650, 737, 2122, 3605, 3607
KflI GGGWCCC 1 cut(s) 1511
Kpn2I TCCGGA 1 cut(s) 1777
KpnI GGTACC 1 cut(s) 2630
KroI GCCGGC 1 cut(s) 2465
KroNI GCCGGC 1 cut(s) 2467
Ksp22I TGATCA 3 cut(s) 961, 1378, 2448
LguI GCTCTTC 4 cut(s) 1287, 2151, 2604, 3448
LmnI GCTCC 3 cut(s) 205, 572, 1497
MaeI CTAG 7 cut(s) 1458, 1464, 1479, 2105, 2303, 2373, 3165
MaeII ACGT 3 cut(s) 166, 1960, 3361
MaeIII GTNAC 8 cut(s) 167, 400, 1531, 1538, 2708, 2794, 3221, 3557
MbiI CCGCTC 2 cut(s) 461, 3441
MflI RGATCY 5 cut(s) 508, 2107, 3019, 3302, 3498
MhlI GDGCHC 1 cut(s) 3145
MlyI GAGTC 1 cut(s) 1082
MmeI TCCRAC 4 cut(s) 235, 1908, 3389, 3569
Mph1103I ATGCAT 1 cut(s) 280
MroI TCCGGA 1 cut(s) 1777
MroNI GCCGGC 1 cut(s) 2465
MroXI GAANNNNTTC 1 cut(s) 1358
MslI CAYNNNNRTG 5 cut(s) 894, 1386, 1500, 1586, 3553
MspA1I CMGCKG 2 cut(s) 141, 2001
MspI CCGG 5 cut(s) 228, 1778, 2466, 3256, 3736
MspR9I CCNGG 2 cut(s) 2843, 3736
Mva1269I GAATGC 2 cut(s) 2805, 3249
MvaI CCWGG 1 cut(s) 2843
MvnI CGCG 2 cut(s) 414, 3607
NaeI GCCGGC 1 cut(s) 2467
NciI CCSGG 1 cut(s) 3736
NgoMIV GCCGGC 1 cut(s) 2465
NlaIV GGNNCC 8 cut(s) 150, 1096, 1512, 1513, 1857, 2346, 2628, 3394
NmeAIII GCCGAG 1 cut(s) 3481
NmuCI GTSAC 4 cut(s) 167, 400, 3221, 3557
NruI TCGCGA 1 cut(s) 414
NsbI TGCGCA 1 cut(s) 651
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 3 cut(s) 2144, 2561, 3620
OliI CACNNNNGTG 2 cut(s) 1386, 3553
PaeI GCATGC 1 cut(s) 2561
PagI TCATGA 1 cut(s) 2173
PauI GCGCGC 1 cut(s) 3605
PceI AGGCCT 1 cut(s) 3285
PciI ACATGT 1 cut(s) 3616
PciSI GCTCTTC 4 cut(s) 1287, 2151, 2604, 3448
PctI GAATGC 2 cut(s) 2805, 3249
PdiI GCCGGC 1 cut(s) 2467
PdmI GAANNNNTTC 1 cut(s) 1358
PfeI GAWTC 6 cut(s) 554, 850, 994, 1128, 1545, 1691
PflFI GACNNNGTC 1 cut(s) 1326
PleI GAGTC 1 cut(s) 1082
PpsI GAGTC 1 cut(s) 1082
PpuMI RGGWCCY 1 cut(s) 1511
PscI ACATGT 1 cut(s) 3616
Psp5II RGGWCCY 1 cut(s) 1511
Psp6I CCWGG 1 cut(s) 2841
PspGI CCWGG 1 cut(s) 2841
PspN4I GGNNCC 8 cut(s) 150, 1096, 1512, 1513, 1857, 2346, 2628, 3394
PspPI GGNCC 5 cut(s) 1217, 1511, 2344, 3392, 3733
PspPPI RGGWCCY 1 cut(s) 1511
PstI CTGCAG 2 cut(s) 534, 3312
PsuI RGATCY 5 cut(s) 508, 2107, 3019, 3302, 3498
PsyI GACNNNGTC 1 cut(s) 1326
PteI GCGCGC 1 cut(s) 3605
PvuII CAGCTG 2 cut(s) 141, 2001
RruI TCGCGA 1 cut(s) 414
RsaI GTAC 5 cut(s) 1462, 2614, 2628, 2914, 3076
RsaNI GTAC 5 cut(s) 1461, 2613, 2627, 2913, 3075
RseI CAYNNNNRTG 5 cut(s) 894, 1386, 1500, 1586, 3553
SapI GCTCTTC 4 cut(s) 1287, 2151, 2604, 3448
Sau96I GGNCC 5 cut(s) 1217, 1511, 2344, 3392, 3733
ScaI AGTACT 1 cut(s) 1462
SchI GAGTC 1 cut(s) 1082
ScrFI CCNGG 2 cut(s) 2843, 3736
SduI GDGCHC 1 cut(s) 3145
SfcI CTRYAG 3 cut(s) 530, 3132, 3308
SinI GGWCC 3 cut(s) 1511, 2344, 3392
SmiMI CAYNNNNRTG 5 cut(s) 894, 1386, 1500, 1586, 3553
SmlI CTYRAG 5 cut(s) 431, 702, 3000, 3263, 3787
SmoI CTYRAG 5 cut(s) 431, 702, 3000, 3263, 3787
SpeI ACTAGT 2 cut(s) 1463, 1478
SphI GCATGC 1 cut(s) 2561
SseBI AGGCCT 1 cut(s) 3285
SsiI CCGC 6 cut(s) 260, 459, 621, 2300, 3439, 3779
SspI AATATT 1 cut(s) 2515
SspMI CTAG 7 cut(s) 1458, 1464, 1479, 2105, 2303, 2373, 3165
StuI AGGCCT 1 cut(s) 3285
StyD4I CCNGG 2 cut(s) 2841, 3734
StyI CCWWGG 4 cut(s) 1940, 2956, 3388, 3729
TaiI ACGT 3 cut(s) 169, 1963, 3364
TaqII GACCGA 2 cut(s) 2332, 3117
TatI WGTACW 1 cut(s) 1460
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 6 cut(s) 554, 850, 994, 1128, 1545, 1691
TscAI CASTG 7 cut(s) 739, 2063, 2221, 2614, 3436, 3555, 3694
TseFI GTSAC 4 cut(s) 167, 400, 3221, 3557
Tsp45I GTSAC 4 cut(s) 167, 400, 3221, 3557
TspGWI ACGGA 6 cut(s) 375, 1003, 1133, 1705, 1816, 2157
TspRI CASTG 7 cut(s) 739, 2063, 2221, 2614, 3436, 3555, 3694
Tth111I GACNNNGTC 1 cut(s) 1326
VpaK11BI GGWCC 3 cut(s) 1511, 2344, 3392
XagI CCTNNNNNAGG 1 cut(s) 3728
XapI RAATTY 2 cut(s) 249, 2588
XceI RCATGY 3 cut(s) 2144, 2561, 3620
XcmI CCANNNNNNNNNTGG 6 cut(s) 923, 1081, 1866, 2651, 2954, 3763
XmnI GAANNNNTTC 1 cut(s) 1358
XspI CTAG 7 cut(s) 1458, 1464, 1479, 2105, 2303, 2373, 3165
ZrmI AGTACT 1 cut(s) 1462
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.