MD14G1189500.v1.1

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000889
Physical Location & Seq
Forward (+)
28103347 .. 28107106
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1189500.v1.1.491

Sequence Viewer

Length: 3102 bp
ATGGAAGTATCCCAAATGTTCAACCAAAACTTGTGTGTGTTTTATCTCTTCGATTCTTCCACCCTCCCCAATGTTTCCCATGCCATTACTCTCAAACTTGATACCACCAACTACCCTCTCTGGCTCGCTCAGATTGTACCTGTTCTCAAGAGTCGTCGTCTGATGCATTTTATTGATGGATCCTCCGAATGCCCTGCCCACTTTCTCAAGGATGATATTGGAAACCTCACCACCAATGTCAATCCTGCTTATGAGGATTGGATTCAGAAAGATCAATTGGTTCTCTCTTGGATCAATGGGTCTCTTGGTCTACAGGCTCTCTCTGCAACAGCCCGTTACAAATCTGCTCGTGATGTCTGGGTTGCTTTGCAACACCGATATGCGTCTCACAATCAGAGTCGCATCATCCAACTTCGTACAGCTTTCTTCCAAACCAAGCGTGGTGATTCCTCCATCGCTGACTATTTGGATCGCATCCACTCACTTGCTGACAGTCTGGCGCTTGCTGGGTCGCCAATCACTGATGCCGATCTTGTTGCCGTGATCATGTCCAATGTTGGACCCAAGTTCGAAAATACAGTTGCTGCTGCCCAAGCTCGTGAAACCCCAGTTTCTTATGTTGATTTGGAAGCCCTGCTCCTCTCTGCTGAGTTGCGTCTTGCCGATCCGACTCCTCCTGTTGATCACTCCGTTCAAGCTATGGCTGCCTCCCGGAATCGCAATTCCTCTCGCGGGGGTGGTCGATCCTTCTCCGTTCGCAATGATGGTCGCTCTTCTCGCGGGAGTGGCTTCTATAAAGGCAATTCCTCTGGACAATTTCATCGTGGTGAACGCAGTGGTGGATTCTATCGAGGTAATTCTAATTCCTCTGGTAATTCTAATCGTGGTCAGCTAGCTGCAGGTCTTTTAGGCCCCGCCCCAAATCACCACAATGGTTCCTCTTCTTCAACCTTTAGTGAATCACGCATAAAGTGTCAAATTTGTGGTAGTCTTGGGCACCCTGCCATTGATTGTTATGACCGTATGAACTTGGCGTATGAAGGGCGTATTCCTTCAAAACGCCTCACTGCCATGGTGGCTCAATCTGTGGCTCAATCCCCTCAAACCGACAAATCTTGGCTTCTAGATTCCGGTGCTAACTCTCACATCACCAATGACTTGGGCAAACTCACTCTTACCCGAGATTATCGTGGCAATGATACCGTCGGTGGTGTGCTTGGTGGAACAGGTTTGCCTATTAGTCATATCGGTAACTCAACCTTATCATCCAATAACCTGACTTTTAAATTGAAAGATGTTCTTCACTCTCCTAATACTTCTACCACCCAATCCTCTCCTATCCCGAACCCAAATGCCCCTCCTCCTCTCCAAGTTTATCACCGTAAAACCCATCGTCCTACAAGCCTTGCACCCTTTCCCGTTACTGATTTCACTACACATACCCAACCTGTTTCTCCAAGTTCCTCAAGACAACCACCTATCCCCGCCACGTCTTCGGTAAGAGCGACGAATGCTCACCCCATGGTGACTCGGAGTAAAGCTGGTAATCTTCAGTCTAATTCCAAATGTGCTCATTATGCGGATTCTTCTGTATCTACTAATACTTTCATTGAGCCTACTTGTTTCAGTCAAGCAAACAAAATTCCTGAGTGGCGTCAAGCCATGGCAGATGAATTCAATGCTCTTCAACGAGCCCGTACCTGGGTCCTTGTTCCCCGTACTCAGTCAATGAACATTTTGCCAAACAAATGGGTCTTCCGTATCAAGAGGAACTCTGATGGCTCTATTCAACGTTTCAAAGCCCGCCTTGCTGCCAATGGATTCCATCAGCAGCCTGGACTCGATTATGGTGAAACCTTTAGTCTTGTGGTCAACCATTCCACCATTCGTCTCATCCTTGCACTATCCGTTCAGTTTCGGTGGCCAGTCCGTCAACTTGACGTTCAAAATGCATTTTTACATGGTTTTTTATCTGAAGAAGTCTATATGCGCCAACCAAATGGTTTTGTGGATTCCCAATTCCCTGATCATGTTTGTAAACTTCAACGATCTCTTTATGGCCTCAAACAAGCCCCTCGGGCGTGGTTTCAATGCTTCTCTTCGCACTTGGAAGACTTGGGTTTCACACCATCTCAGGCTGACACATCTCTTTTCATTTATCTTAATGGATCCATACGCATTTATCTGCTTATCTATGTCGATGATATTTTGGTCACAGGGAATGATATGTCTCATATTGCTCGGTTGATTGCTGACTTAGGACGTCGCTTCGCTATGAAAGATTTAGGCCCTGCTCATTATTTCCTCGGCATGGAGATTGTCCGCACATCGGATGGCTTATTTCTTTCTCAACAAAAGTATGTACAGGATCTTCTATTGCGTACCAAAATGGCAGCAGCTAAACCTGTTCATACCCCAGCTGTTAGCGGTCGCAGGCTCAGCCTTCAAGATGGAGACCCTCTTCCTGATCCGACAGAATACCGAAGTGTTGTAGGTGCTCTCCAATACCTTACTCTCACTCGTCCTGACATTGCATTTGCGGTCAACCAAGTCTGTCAATTTATGCATCAACCCACTTCTATCCATTGGTTGGCTGTCAAACGTATCCTACGATATTTGGTTGGTACTTCCTCTCACGGTATTACATATAAACCTGGCTCCCTTATTCTCACTGCTTATTCTGATGCTGATTATGCCGGTGATCCTGATGACCGGCGTTCCACTGGTGGATATTGCATTTATATTGGTTCAAACCTTGTCTCTTGGAGCTCCAAGAAACAAGGTGGGGTTTCCCGTTCAAGTACCGAAGCGGAATACCGCCAGCTTGCCTACACTGCTGCTGCAATTTCATGGTTCCGCAAGCTTTTTCGTGATCTTTGCCTTCCGCTGTCTTGTCCAACAGTTCATTGTGATAATATTAGTGCCATATCCTTAGCCTCAAATCCTGTTTTTCATGCCCGTACTCGTCATGTTGAGCACATGCATGGTTTAGGAGTGGGATGTCCTACTGTCTTGGACACTTTTCAAAACATTGCACACACGGGAAATCTTTTTGTGTTAAATTTTATGATCTTAAATAGAGTGTTTTTTTTTTTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2

Protein Analysis

1034

Amino Acids

114.21

Weight (kDa)

9.37

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 49 - 217 2.8e-17 gag-polypeptide of LTR copia-type
RVT_2 PF07727 566 - 808 2.6e-67 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2266
Acc36I ACCTGC 1 cut(s) 890
AccB1I GGYRCC 1 cut(s) 996
AccB7I CCANNNNNTGG 1 cut(s) 2590
AccI GTMKAC 1 cut(s) 310
AccII CGCG 2 cut(s) 732, 780
AclI AACGTT 1 cut(s) 1792
AcoI YGGCCR 1 cut(s) 1922
AcsI RAATTY 4 cut(s) 978, 1641, 1673, 3061
AcuI CTGAAG 2 cut(s) 1535, 1995
AcyI GRCGYC 2 cut(s) 1654, 2263
AfaI GTAC 9 cut(s) 138, 418, 1699, 1720, 2364, 2383, 2626, 2803, 2962
AfiI CCNNNNNNNGG 6 cut(s) 732, 1701, 1702, 2311, 2329, 2590
AjiI CACGTC 1 cut(s) 1491
AjnI CCWGG 3 cut(s) 1700, 1834, 2653
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
Alw21I GWGCWC 4 cut(s) 1573, 2500, 2771, 2979
Alw26I GTCTC 6 cut(s) 306, 390, 1895, 2235, 2448, 2764
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 2 cut(s) 1179, 2076
AoxI GGCC 4 cut(s) 910, 1922, 2059, 2287
ApoI RAATTY 4 cut(s) 978, 1641, 1673, 3061
ArsI GACNNNNNNTTYG 2 cut(s) 2519, 2551
AspLEI GCGC 2 cut(s) 502, 1992
AspS9I GGNCC 4 cut(s) 560, 911, 1705, 2288
AsuC2I CCSGG 1 cut(s) 712
AsuII TTCGAA 1 cut(s) 570
AsuNHI GCTAGC 1 cut(s) 892
AvaI CYCGRG 2 cut(s) 1179, 2076
AvaII GGWCC 2 cut(s) 560, 1705
BaeGI GKGCMC 1 cut(s) 999
BalI TGGCCA 1 cut(s) 1924
BamHI GGATCC 2 cut(s) 179, 2168
BanI GGYRCC 1 cut(s) 996
BanII GRGCYC 2 cut(s) 1696, 2771
BarI GAAGNNNNNNTAC 2 cut(s) 2355, 2387
BauI CACGAG 2 cut(s) 348, 597
BbsI GAAGAC 3 cut(s) 1485, 1747, 2118
Bbv12I GWGCWC 4 cut(s) 1573, 2500, 2771, 2979
BccI CCATC 9 cut(s) 170, 461, 758, 1398, 1772, 1833, 2137, 2327, 2444
BceAI ACGGC 1 cut(s) 524
BcgI CGANNNNNNTGC 4 cut(s) 176, 210, 518, 552
BciT130I CCWGG 3 cut(s) 1702, 1836, 2655
BciVI GTATCC 2 cut(s) 19, 2615
BclI TGATCA 3 cut(s) 543, 682, 2026
BcnI CCSGG 1 cut(s) 712
BcoDI GTCTC 6 cut(s) 306, 390, 1895, 2235, 2448, 2764
BfaI CTAG 2 cut(s) 893, 1124
BfmI CTRYAG 2 cut(s) 311, 897
BfoI RGCGCY 1 cut(s) 503
BfuAI ACCTGC 1 cut(s) 890
BfuI GTATCC 2 cut(s) 19, 2615
BglI GCCNNNNNGGC 2 cut(s) 1076, 2078
BlpI GCTNAGC 1 cut(s) 2438
Bme1390I CCNGG 4 cut(s) 712, 1702, 1836, 2655
Bme18I GGWCC 2 cut(s) 560, 1705
BmeT110I CYCGRG 2 cut(s) 1179, 2076
BmgBI CACGTC 1 cut(s) 1491
BmgT120I GGNCC 4 cut(s) 560, 911, 1705, 2288
BmiI GGNNCC 9 cut(s) 181, 562, 913, 937, 998, 1706, 2170, 2659, 2855
BmrFI CCNGG 4 cut(s) 712, 1702, 1836, 2655
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 6 cut(s) 153, 411, 483, 514, 2575, 2674
BmtI GCTAGC 1 cut(s) 896
BmuI ACTGGG 1 cut(s) 602
BpiI GAAGAC 3 cut(s) 1485, 1747, 2118
Bpu10I CCTNAGC 1 cut(s) 2932
Bpu1102I GCTNAGC 1 cut(s) 2438
Bpu14I TTCGAA 1 cut(s) 570
BpuEI CTTGAG 3 cut(s) 131, 191, 1450
BpuMI CCSGG 1 cut(s) 712
BsaBI GATNNNNATC 1 cut(s) 528
BsaHI GRCGYC 2 cut(s) 1654, 2263
BsaI GGTCTC 2 cut(s) 306, 2448
BsaJI CCNNGG 6 cut(s) 1071, 1521, 1662, 1701, 2075, 2305
BsaWI WCCGGW 1 cut(s) 1130
Bsc4I CCNNNNNNNGG 6 cut(s) 732, 1701, 1702, 2311, 2329, 2590
Bse118I RCCGGY 2 cut(s) 2696, 2712
Bse1I ACTGG 3 cut(s) 608, 1925, 2728
Bse3DI GCAATG 4 cut(s) 766, 1201, 2529, 3030
Bse8I GATNNNNATC 1 cut(s) 528
BseBI CCWGG 3 cut(s) 1702, 1836, 2655
BseDI CCNNGG 6 cut(s) 1071, 1521, 1662, 1701, 2075, 2305
BseGI GGATG 7 cut(s) 217, 405, 474, 1265, 1893, 2338, 3005
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 6 cut(s) 732, 1701, 1702, 2311, 2329, 2590
BseMI GCAATG 4 cut(s) 766, 1201, 2529, 3030
BseMII CTCAG 6 cut(s) 143, 639, 1638, 1736, 2147, 2452
BseNI ACTGG 3 cut(s) 608, 1925, 2728
BseRI GAGGAG 4 cut(s) 629, 663, 1350, 1353
BseSI GKGCMC 1 cut(s) 999
BseYI CCCAGC 2 cut(s) 506, 2416
Bsh1236I CGCG 2 cut(s) 732, 780
Bsh1285I CGRYCG 1 cut(s) 2431
BshFI GGCC 4 cut(s) 912, 1924, 2061, 2289
BshNI GGYRCC 1 cut(s) 996
BsiEI CGRYCG 1 cut(s) 2431
BsiHKAI GWGCWC 4 cut(s) 1573, 2500, 2771, 2979
BsiHKCI CYCGRG 2 cut(s) 1179, 2076
BsiSI CCGG 4 cut(s) 712, 1131, 2697, 2713
BslI CCNNNNNNNGG 6 cut(s) 732, 1701, 1702, 2311, 2329, 2590
BsmAI GTCTC 6 cut(s) 306, 390, 1895, 2235, 2448, 2764
BsmBI CGTCTC 2 cut(s) 390, 1895
BsmI GAATGC 2 cut(s) 194, 1516
BsnI GGCC 4 cut(s) 912, 1924, 2061, 2289
Bso31I GGTCTC 2 cut(s) 306, 2448
BsoBI CYCGRG 2 cut(s) 1179, 2076
Bsp119I TTCGAA 1 cut(s) 570
Bsp1286I GDGCHC 6 cut(s) 999, 1573, 1696, 2500, 2771, 2979
Bsp1407I TGTACA 1 cut(s) 2362
Bsp1720I GCTNAGC 1 cut(s) 2438
Bsp19I CCATGG 3 cut(s) 1071, 1521, 1662
BspANI GGCC 4 cut(s) 912, 1924, 2061, 2289
BspCNI CTCAG 6 cut(s) 142, 640, 1639, 1735, 2146, 2451
BspFNI CGCG 2 cut(s) 732, 780
BspLI GGNNCC 9 cut(s) 181, 562, 913, 937, 998, 1706, 2170, 2659, 2855
BspMAI CTGCAG 1 cut(s) 901
BspMI ACCTGC 1 cut(s) 890
BspOI GCTAGC 1 cut(s) 896
BspQI GCTCTTC 2 cut(s) 778, 1689
BspT104I TTCGAA 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 996
BspTNI GGTCTC 2 cut(s) 306, 2448
BsrDI GCAATG 4 cut(s) 766, 1201, 2529, 3030
BsrFI RCCGGY 2 cut(s) 2696, 2712
BsrGI TGTACA 1 cut(s) 2362
BsrI ACTGG 3 cut(s) 608, 1925, 2728
BssAI RCCGGY 2 cut(s) 2696, 2712
BssECI CCNNGG 6 cut(s) 1071, 1521, 1662, 1701, 2075, 2305
BssNI GRCGYC 2 cut(s) 1654, 2263
BssSI CACGAG 2 cut(s) 348, 597
BssT1I CCWWGG 3 cut(s) 1071, 1521, 1662
Bst2BI CACGAG 2 cut(s) 348, 597
Bst2UI CCWGG 3 cut(s) 1702, 1836, 2655
Bst4CI ACNGT 8 cut(s) 494, 580, 1022, 1204, 1382, 2639, 2902, 3010
Bst6I CTCTTC 6 cut(s) 53, 778, 946, 1689, 2104, 2466
BstACI GRCGYC 2 cut(s) 1654, 2263
BstAUI TGTACA 1 cut(s) 2362
BstBI TTCGAA 1 cut(s) 570
BstC8I GCNNGC 8 cut(s) 126, 504, 894, 1804, 2435, 2822, 2826, 2861
BstDEI CTNAG 8 cut(s) 129, 648, 1647, 1722, 2133, 2257, 2438, 2932
BstDSI CCRYGG 3 cut(s) 1071, 1521, 1662
BstF5I GGATG 7 cut(s) 217, 405, 474, 1265, 1893, 2338, 3005
BstFNI CGCG 2 cut(s) 732, 780
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 2 cut(s) 502, 1992
BstMAI GTCTC 6 cut(s) 306, 390, 1895, 2235, 2448, 2764
BstMCI CGRYCG 1 cut(s) 2431
BstNI CCWGG 3 cut(s) 1702, 1836, 2655
BstNSI RCATGY 1 cut(s) 2983
BstSCI CCNGG 4 cut(s) 710, 1700, 1834, 2653
BstSFI CTRYAG 2 cut(s) 311, 897
BstSLI GKGCMC 1 cut(s) 999
BstUI CGCG 2 cut(s) 732, 780
BstV2I GAAGAC 3 cut(s) 1485, 1747, 2118
BstX2I RGATCY 3 cut(s) 179, 2168, 2368
BstXI CCANNNNNNTGG 3 cut(s) 1159, 1749, 2000
BstYI RGATCY 3 cut(s) 179, 2168, 2368
BsuI GTATCC 2 cut(s) 19, 2615
BsuRI GGCC 4 cut(s) 912, 1924, 2061, 2289
BtgI CCRYGG 3 cut(s) 1071, 1521, 1662
BtgZI GCGATG 1 cut(s) 439
BtrI CACGTC 1 cut(s) 1491
BtsCI GGATG 7 cut(s) 217, 405, 474, 1265, 1893, 2338, 3005
BtsI GCAGTG 4 cut(s) 841, 1065, 2670, 2832
BtsIMutI CAGTG 6 cut(s) 519, 841, 1065, 2670, 2721, 2832
BveI ACCTGC 1 cut(s) 890
Cac8I GCNNGC 8 cut(s) 126, 504, 894, 1804, 2435, 2822, 2826, 2861
CaiI CAGNNNCTG 1 cut(s) 584
CfoI GCGC 2 cut(s) 502, 1992
Cfr10I RCCGGY 2 cut(s) 2696, 2712
Cfr13I GGNCC 4 cut(s) 560, 911, 1705, 2288
CseI GACGC 3 cut(s) 372, 644, 1643
Csp6I GTAC 9 cut(s) 137, 417, 1698, 1719, 2363, 2382, 2625, 2802, 2961
CviQI GTAC 9 cut(s) 137, 417, 1698, 1719, 2363, 2382, 2625, 2802, 2961
DdeI CTNAG 8 cut(s) 129, 648, 1647, 1722, 2133, 2257, 2438, 2932
DraI TTTAAA 2 cut(s) 1285, 3097
EaeI YGGCCR 1 cut(s) 1922
Eam1104I CTCTTC 6 cut(s) 53, 778, 946, 1689, 2104, 2466
EarI CTCTTC 6 cut(s) 53, 778, 946, 1689, 2104, 2466
Ecl136II GAGCTC 1 cut(s) 2769
Eco130I CCWWGG 3 cut(s) 1071, 1521, 1662
Eco24I GRGCYC 2 cut(s) 1696, 2771
Eco31I GGTCTC 2 cut(s) 306, 2448
Eco47I GGWCC 2 cut(s) 560, 1705
Eco53kI GAGCTC 1 cut(s) 2769
Eco57I CTGAAG 2 cut(s) 1535, 1995
Eco88I CYCGRG 2 cut(s) 1179, 2076
EcoICRI GAGCTC 1 cut(s) 2769
EcoO109I RGGNCCY 3 cut(s) 911, 1705, 2288
EcoRI GAATTC 1 cut(s) 1673
EcoRII CCWGG 3 cut(s) 1700, 1834, 2653
EcoT14I CCWWGG 3 cut(s) 1071, 1521, 1662
EcoT22I ATGCAT 4 cut(s) 168, 1954, 2568, 2985
EcoT38I GRGCYC 2 cut(s) 1696, 2771
ErhI CCWWGG 3 cut(s) 1071, 1521, 1662
Esp3I CGTCTC 2 cut(s) 390, 1895
FalI AAGNNNNNCTT 4 cut(s) 1284, 1316, 1791, 1823
FauI CCCGC 5 cut(s) 725, 773, 922, 1492, 1811
FbaI TGATCA 3 cut(s) 543, 682, 2026
FblI GTMKAC 1 cut(s) 310
FokI GGATG 7 cut(s) 224, 392, 461, 1252, 1880, 2345, 3012
FriOI GRGCYC 2 cut(s) 1696, 2771
FspBI CTAG 2 cut(s) 893, 1124
GlaI GCGC 2 cut(s) 501, 1991
GsaI CCCAGC 2 cut(s) 510, 2420
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 4 cut(s) 912, 1924, 2061, 2289
HapII CCGG 4 cut(s) 712, 1131, 2697, 2713
HgaI GACGC 3 cut(s) 372, 644, 1643
HhaI GCGC 2 cut(s) 502, 1992
Hin1I GRCGYC 2 cut(s) 1654, 2263
Hin6I GCGC 2 cut(s) 500, 1990
HinP1I GCGC 2 cut(s) 500, 1990
HincII GTYRAC 3 cut(s) 1873, 1934, 2545
HindII GTYRAC 3 cut(s) 1873, 1934, 2545
HindIII AAGCTT 1 cut(s) 2861
HpaII CCGG 4 cut(s) 712, 1131, 2697, 2713
Hpy166II GTNNAC 6 cut(s) 311, 830, 1873, 1934, 2039, 2545
Hpy8I GTNNAC 6 cut(s) 311, 830, 1873, 1934, 2039, 2545
Hpy99I CGWCG 4 cut(s) 159, 1208, 1510, 2268
HpyAV CCTTC 5 cut(s) 757, 1034, 1062, 2453, 2891
HpyCH4III ACNGT 8 cut(s) 494, 580, 1022, 1204, 1382, 2639, 2902, 3010
HpyCH4IV ACGT 5 cut(s) 1490, 1792, 1941, 2263, 2602
HpyF3I CTNAG 8 cut(s) 129, 648, 1647, 1722, 2133, 2257, 2438, 2932
HpySE526I ACGT 5 cut(s) 1490, 1792, 1941, 2263, 2602
Hsp92I GRCGYC 2 cut(s) 1654, 2263
HspAI GCGC 2 cut(s) 500, 1990
Ksp22I TGATCA 3 cut(s) 543, 682, 2026
LguI GCTCTTC 2 cut(s) 778, 1689
LmnI GCTCC 4 cut(s) 642, 2663, 2766, 2774
LweI GCATC 6 cut(s) 153, 411, 483, 514, 2575, 2674
MaeI CTAG 2 cut(s) 893, 1124
MaeII ACGT 5 cut(s) 1490, 1792, 1941, 2263, 2602
MaeIII GTNAC 5 cut(s) 335, 1250, 1420, 1525, 2212
MfeI CAATTG 1 cut(s) 275
MflI RGATCY 3 cut(s) 179, 2168, 2368
MhlI GDGCHC 6 cut(s) 999, 1573, 1696, 2500, 2771, 2979
MlsI TGGCCA 1 cut(s) 1924
MluNI TGGCCA 1 cut(s) 1924
MlyI GAGTC 5 cut(s) 160, 406, 664, 1522, 1833
MmeI TCCRAC 5 cut(s) 433, 538, 692, 2495, 2921
Mox20I TGGCCA 1 cut(s) 1924
Mph1103I ATGCAT 4 cut(s) 168, 1954, 2568, 2985
MscI TGGCCA 1 cut(s) 1924
MseI TTAA 5 cut(s) 1284, 2163, 3059, 3074, 3096
MslI CAYNNNNRTG 5 cut(s) 378, 825, 930, 1070, 2982
Msp20I TGGCCA 1 cut(s) 1924
MspA1I CMGCKG 2 cut(s) 2420, 2887
MspI CCGG 4 cut(s) 712, 1131, 2697, 2713
MspR9I CCNGG 4 cut(s) 712, 1702, 1836, 2655
MunI CAATTG 1 cut(s) 275
Mva1269I GAATGC 2 cut(s) 194, 1516
MvaI CCWGG 3 cut(s) 1702, 1836, 2655
MvnI CGCG 2 cut(s) 732, 780
NciI CCSGG 1 cut(s) 712
NcoI CCATGG 3 cut(s) 1071, 1521, 1662
NheI GCTAGC 1 cut(s) 892
NlaIV GGNNCC 9 cut(s) 181, 562, 913, 937, 998, 1706, 2170, 2659, 2855
NmeAIII GCCGAG 1 cut(s) 2286
NmuCI GTSAC 2 cut(s) 1525, 2212
NsiI ATGCAT 4 cut(s) 168, 1954, 2568, 2985
NspI RCATGY 1 cut(s) 2983
NspV TTCGAA 1 cut(s) 570
PciSI GCTCTTC 2 cut(s) 778, 1689
PcsI WCGNNNNNNNCGW 2 cut(s) 1502, 2608
PctI GAATGC 2 cut(s) 194, 1516
PflMI CCANNNNNTGG 1 cut(s) 2590
PfoI TCCNGGA 1 cut(s) 710
PleI GAGTC 5 cut(s) 159, 405, 664, 1522, 1833
PpsI GAGTC 5 cut(s) 159, 405, 664, 1522, 1833
PpuMI RGGWCCY 1 cut(s) 1705
Psp124BI GAGCTC 1 cut(s) 2771
Psp1406I AACGTT 1 cut(s) 1792
Psp5II RGGWCCY 1 cut(s) 1705
Psp6I CCWGG 3 cut(s) 1700, 1834, 2653
PspFI CCCAGC 2 cut(s) 506, 2416
PspGI CCWGG 3 cut(s) 1700, 1834, 2653
PspN4I GGNNCC 9 cut(s) 181, 562, 913, 937, 998, 1706, 2170, 2659, 2855
PspPI GGNCC 4 cut(s) 560, 911, 1705, 2288
PspPPI RGGWCCY 1 cut(s) 1705
PstI CTGCAG 1 cut(s) 901
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 3 cut(s) 179, 2168, 2368
PvuII CAGCTG 1 cut(s) 2420
RsaI GTAC 9 cut(s) 138, 418, 1699, 1720, 2364, 2383, 2626, 2803, 2962
RsaNI GTAC 9 cut(s) 137, 417, 1698, 1719, 2363, 2382, 2625, 2802, 2961
RseI CAYNNNNRTG 5 cut(s) 378, 825, 930, 1070, 2982
SacI GAGCTC 1 cut(s) 2771
SapI GCTCTTC 2 cut(s) 778, 1689
SaqAI TTAA 5 cut(s) 1284, 2163, 3059, 3074, 3096
Sau96I GGNCC 4 cut(s) 560, 911, 1705, 2288
SchI GAGTC 5 cut(s) 160, 406, 664, 1522, 1833
ScrFI CCNGG 4 cut(s) 712, 1702, 1836, 2655
SduI GDGCHC 6 cut(s) 999, 1573, 1696, 2500, 2771, 2979
SfaNI GCATC 6 cut(s) 153, 411, 483, 514, 2575, 2674
SfcI CTRYAG 2 cut(s) 311, 897
SfuI TTCGAA 1 cut(s) 570
SinI GGWCC 2 cut(s) 560, 1705
SmiMI CAYNNNNRTG 5 cut(s) 378, 825, 930, 1070, 2982
SmlI CTYRAG 3 cut(s) 146, 206, 1465
SmoI CTYRAG 3 cut(s) 146, 206, 1465
SspI AATATT 1 cut(s) 2917
SspMI CTAG 2 cut(s) 893, 1124
SstI GAGCTC 1 cut(s) 2771
StyD4I CCNGG 4 cut(s) 710, 1700, 1834, 2653
StyI CCWWGG 3 cut(s) 1071, 1521, 1662
TaaI ACNGT 8 cut(s) 494, 580, 1022, 1204, 1382, 2639, 2902, 3010
TaiI ACGT 5 cut(s) 1493, 1795, 1944, 2266, 2605
TaqI TCGA 6 cut(s) 51, 570, 742, 850, 1842, 2199
TatI WGTACW 1 cut(s) 2362
Tru1I TTAA 5 cut(s) 1284, 2163, 3059, 3074, 3096
Tru9I TTAA 5 cut(s) 1284, 2163, 3059, 3074, 3096
TscAI CASTG 6 cut(s) 526, 841, 1072, 2677, 2728, 2839
TseFI GTSAC 2 cut(s) 1525, 2212
Tsp45I GTSAC 2 cut(s) 1525, 2212
TspGWI ACGGA 5 cut(s) 679, 742, 1748, 1897, 1919
TspRI CASTG 6 cut(s) 526, 841, 1072, 2677, 2728, 2839
Van91I CCANNNNNTGG 1 cut(s) 2590
VpaK11BI GGWCC 2 cut(s) 560, 1705
XapI RAATTY 4 cut(s) 978, 1641, 1673, 3061
XbaI TCTAGA 1 cut(s) 1123
XceI RCATGY 1 cut(s) 2983
XcmI CCANNNNNNNNNTGG 2 cut(s) 437, 1832
XmiI GTMKAC 1 cut(s) 310
XspI CTAG 2 cut(s) 893, 1124
ZraI GACGTC 1 cut(s) 2264
Zsp2I ATGCAT 4 cut(s) 168, 1954, 2568, 2985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.