MD13G1157100.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000889
Physical Location & Seq
Forward (+)
12270155 .. 12270633
479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1157100.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGCTTCCGCTAAATGGCTGGAGAACATTATGAAGCCAAGATGGTTGACAAGTCTTCTGTTTGATTTAGGGTTTCACAGTAAGGAACCTATATCTATGTTCTATGACAATCAAGCTGCCATGCATATTGCTTCCAATCCTATATTTCACGAGAGAACGAAGCATATTGAAGTGGATTGTCATTATGTTCGAGCACAGGTTCAAGCCAACATCATCAACACTCATTACACTCGCAGTCATGATCAATTGGCTGACATTTTTACTAAACCTCTTTCTACGGTTCAGTTCATGCGCATTCTGTCCAGGCTTGGCTCAAGGAATCCCCTCGATCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

113

Amino Acids

13.02

Weight (kDa)

8.89

Isoelectric Point (pI)

64.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 293
AciI CCGC 1 cut(s) 9
AclWI GGATC 1 cut(s) 323
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 2 cut(s) 170, 203
AjnI CCWGG 1 cut(s) 302
AluBI AGCT 2 cut(s) 116, 335
AluI AGCT 2 cut(s) 116, 335
Alw21I GWGCWC 1 cut(s) 196
AlwI GGATC 1 cut(s) 323
ApeKI GCWGC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 294
BauI CACGAG 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 47
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 103
BccI CCATC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 304
BclI TGATCA 1 cut(s) 241
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 1 cut(s) 304
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 298
Bsc4I CCNNNNNNNGG 1 cut(s) 15
BseBI CCWGG 1 cut(s) 304
BseLI CCNNNNNNNGG 1 cut(s) 15
BseXI GCAGC 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 15
BsmI GAATGC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 196
Bsp143I GATC 2 cut(s) 241, 328
BspACI CCGC 1 cut(s) 9
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 87
BspPI GGATC 1 cut(s) 323
BssMI GATC 2 cut(s) 241, 328
BssSI CACGAG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 80, 280
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 2 cut(s) 244, 331
BstMBI GATC 2 cut(s) 241, 328
BstNI CCWGG 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 103
BstV2I GAAGAC 1 cut(s) 47
CciI TCATGA 1 cut(s) 238
CfoI GCGC 1 cut(s) 294
CviAII CATG 3 cut(s) 121, 239, 289
CviJI RGCY 9 cut(s) 5, 19, 37, 116, 206, 251, 307, 312, 335
CviKI_1 RGCY 9 cut(s) 5, 19, 37, 116, 206, 251, 307, 312, 335
DpnI GATC 2 cut(s) 243, 330
DpnII GATC 2 cut(s) 241, 328
EcoRII CCWGG 1 cut(s) 302
EcoT22I ATGCAT 1 cut(s) 126
FaeI CATG 3 cut(s) 124, 242, 292
FatI CATG 3 cut(s) 120, 238, 288
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 1 cut(s) 117
Fsp4HI GCNGC 1 cut(s) 117
FspAI RTGCGCAY 1 cut(s) 293
FspI TGCGCA 1 cut(s) 293
GlaI GCGC 1 cut(s) 293
GluI GCNGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 41
HhaI GCGC 1 cut(s) 294
Hin1II CATG 3 cut(s) 124, 242, 292
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 1 cut(s) 319
Hpy166II GTNNAC 1 cut(s) 48
Hpy188III TCNNGA 2 cut(s) 149, 239
Hpy8I GTNNAC 1 cut(s) 48
HpyCH4III ACNGT 2 cut(s) 80, 280
HpyCH4V TGCA 1 cut(s) 124
Hsp92II CATG 3 cut(s) 124, 242, 292
HspAI GCGC 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 2 cut(s) 241, 328
LpnPI CCDG 4 cut(s) 5, 182, 289, 316
Lsp1109I GCAGC 1 cut(s) 103
MalI GATC 2 cut(s) 243, 330
MboI GATC 2 cut(s) 241, 328
MboII GAAGA 1 cut(s) 47
MfeI CAATTG 1 cut(s) 245
MhlI GDGCHC 1 cut(s) 196
MluCI AATT 1 cut(s) 245
MnlI CCTC 2 cut(s) 279, 335
Mph1103I ATGCAT 1 cut(s) 126
MspR9I CCNGG 1 cut(s) 304
MunI CAATTG 1 cut(s) 245
Mva1269I GAATGC 1 cut(s) 294
MvaI CCWGG 1 cut(s) 304
NdeII GATC 2 cut(s) 241, 328
NlaIII CATG 3 cut(s) 124, 242, 292
NlaIV GGNNCC 1 cut(s) 87
NsbI TGCGCA 1 cut(s) 293
NsiI ATGCAT 1 cut(s) 126
PagI TCATGA 1 cut(s) 238
PctI GAATGC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 1 cut(s) 118
Psp6I CCWGG 1 cut(s) 302
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 87
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 2 cut(s) 241, 328
ScrFI CCNGG 1 cut(s) 304
SduI GDGCHC 1 cut(s) 196
SetI ASST 5 cut(s) 91, 118, 201, 271, 337
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 1 cut(s) 245
SsiI CCGC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 302
TaaI ACNGT 2 cut(s) 80, 280
TaqI TCGA 2 cut(s) 190, 327
TasI AATT 1 cut(s) 245
TfiI GAWTC 1 cut(s) 319
TseI GCWGC 1 cut(s) 116
TspDTI ATGAA 2 cut(s) 47, 277
Zsp2I ATGCAT 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.