pycom08g03700
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000889
Physical Location & Seq
Forward (+)
2852030 .. 2853096
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03700.5

Sequence Viewer

Length: 777 bp
ATGGCATGTGAACTGATACACGTGATGCGTGAGGGATTGAATAATTGGGATATACTCGGGAACAACCAAGAATCTCTCTATGTTCATAAAATCGTTTCAACACAATCTTTTTCAACACGCTCTAAACTTTTATTCAGATTAACAACTATCGCGCATTATTCACTCCACACTTACTTATCGTTCTCCTTGATACAAAGAAAATATGCAATTGATATACTTAAAGACCATGGTTTCTTGGTTGTAAGAGAAGCTACTCAAGGATCCTGCCAAATACTGACGTTTGCACATATTCCGTACGTGTACTTAGTCGGTTTATGCATGAAACGCAGCAGCCCCAAATGGATGCCGCTCTTCGAGTTGTTTGATATCTTAAATCTACTCCTGCCAAGGTTTGTTCTTTCCATCAAACAACAAATTAAATCTAGTTTGTGTTTTCTTGGGCAATTCATTAATTTCATGGTGAATGAAAAGATAGATAACAATTTTTTTTTCTTGGGCAACTCTCTATGGTTGGTACTTGTTGTGTACTTGGCTACATTTTATGTTGGAAGGTTTACAATTGCCGCATTATGGAGCATCTATTTTATATTAAGAGAATTCGGCATATTGAGATGTATTATCATTTCATCATGGACGAGACAGTTGATGGCACAAAAACTCCATGATATGAGAATTCTTCTCAGATTAGCTGATGTTTTTACGAAACCATTAGGCAAGAAAATGTTCTTGGTGATGTTACGCAAGTTGGGAGTTCTTGACATTTGCTCTCCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

259

Amino Acids

30.08

Weight (kDa)

9.65

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 349
AccII CGCG 1 cut(s) 152
AciI CCGC 2 cut(s) 347, 564
AclWI GGATC 2 cut(s) 255, 268
AcsI RAATTY 2 cut(s) 596, 672
AcvI CACGTG 1 cut(s) 22
AfaI GTAC 4 cut(s) 296, 302, 516, 527
AfiI CCNNNNNNNGG 1 cut(s) 570
AflIII ACRYGT 2 cut(s) 19, 297
AgsI TTSAA 3 cut(s) 40, 99, 114
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AluBI AGCT 2 cut(s) 251, 689
AluI AGCT 2 cut(s) 251, 689
Alw26I GTCTC 1 cut(s) 631
AlwI GGATC 2 cut(s) 255, 268
Ama87I CYCGRG 1 cut(s) 56
ApeKI GCWGC 2 cut(s) 327, 330
ApoI RAATTY 2 cut(s) 596, 672
AseI ATTAAT 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 1 cut(s) 154
AsuHPI GGTGA 2 cut(s) 472, 742
AvaI CYCGRG 1 cut(s) 56
BamHI GGATCC 1 cut(s) 260
BbrPI CACGTG 1 cut(s) 22
BbvI GCAGC 2 cut(s) 339, 342
BccI CCATC 2 cut(s) 410, 640
BcoDI GTCTC 1 cut(s) 631
BfaI CTAG 1 cut(s) 423
BisI GCNGC 4 cut(s) 328, 331, 347, 564
BlsI GCNGC 4 cut(s) 329, 332, 348, 565
BmeT110I CYCGRG 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 262
BmsI GCATC 3 cut(s) 15, 333, 585
BpuEI CTTGAG 1 cut(s) 240
BsaAI YACGTR 2 cut(s) 22, 298
BsaJI CCNNGG 2 cut(s) 226, 386
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 226, 386
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 694
BseXI GCAGC 2 cut(s) 339, 342
Bsh1236I CGCG 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 56
BsiWI CGTACG 1 cut(s) 294
BslI CCNNNNNNNGG 1 cut(s) 570
BsmAI GTCTC 1 cut(s) 631
BsoBI CYCGRG 1 cut(s) 56
Bsp143I GATC 1 cut(s) 260
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 2 cut(s) 347, 564
BspCNI CTCAG 1 cut(s) 693
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 262
BspPI GGATC 2 cut(s) 255, 268
BspQI GCTCTTC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 226, 386
BssMI GATC 1 cut(s) 260
BssT1I CCWWGG 2 cut(s) 226, 386
Bst4CI ACNGT 1 cut(s) 642
Bst6I CTCTTC 1 cut(s) 356
BstBAI YACGTR 2 cut(s) 22, 298
BstDEI CTNAG 2 cut(s) 304, 680
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 348
BstFNI CGCG 1 cut(s) 152
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 1 cut(s) 263
BstMAI GTCTC 1 cut(s) 631
BstMBI GATC 1 cut(s) 260
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 9
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 2 cut(s) 339, 342
BstX2I RGATCY 1 cut(s) 260
BstYI RGATCY 1 cut(s) 260
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 1 cut(s) 348
CfoI GCGC 1 cut(s) 154
Csp6I GTAC 4 cut(s) 295, 301, 515, 526
CviAII CATG 6 cut(s) 6, 227, 319, 457, 630, 662
CviJI RGCY 4 cut(s) 251, 333, 533, 689
CviKI_1 RGCY 4 cut(s) 251, 333, 533, 689
CviQI GTAC 4 cut(s) 295, 301, 515, 526
DdeI CTNAG 2 cut(s) 304, 680
DpnI GATC 1 cut(s) 262
DpnII GATC 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 226, 386
Eco32I GATATC 1 cut(s) 367
Eco72I CACGTG 1 cut(s) 22
Eco88I CYCGRG 1 cut(s) 56
EcoRI GAATTC 2 cut(s) 596, 672
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 2 cut(s) 226, 386
EcoT22I ATGCAT 1 cut(s) 320
ErhI CCWWGG 2 cut(s) 226, 386
FaeI CATG 6 cut(s) 9, 230, 322, 460, 633, 665
FatI CATG 6 cut(s) 5, 226, 318, 456, 629, 661
Fnu4HI GCNGC 4 cut(s) 328, 331, 347, 564
FokI GGATG 1 cut(s) 355
Fsp4HI GCNGC 4 cut(s) 328, 331, 347, 564
FspBI CTAG 1 cut(s) 423
GlaI GCGC 1 cut(s) 153
GluI GCNGC 4 cut(s) 328, 331, 347, 564
HhaI GCGC 1 cut(s) 154
Hin1II CATG 6 cut(s) 9, 230, 322, 460, 633, 665
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HinfI GANTC 1 cut(s) 71
HphI GGTGA 2 cut(s) 472, 742
Hpy166II GTNNAC 4 cut(s) 11, 301, 526, 555
Hpy188I TCNGA 2 cut(s) 137, 683
Hpy188III TCNNGA 2 cut(s) 58, 755
Hpy8I GTNNAC 4 cut(s) 11, 301, 526, 555
HpyAV CCTTC 1 cut(s) 543
HpyCH4III ACNGT 1 cut(s) 642
HpyCH4IV ACGT 3 cut(s) 21, 278, 297
HpyCH4V TGCA 3 cut(s) 206, 284, 318
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpyF3I CTNAG 2 cut(s) 304, 680
HpySE526I ACGT 3 cut(s) 21, 278, 297
Hsp92II CATG 6 cut(s) 9, 230, 322, 460, 633, 665
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 1 cut(s) 260
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 1 cut(s) 573
LpnPI CCDG 2 cut(s) 277, 395
Lsp1109I GCAGC 2 cut(s) 339, 342
LweI GCATC 3 cut(s) 15, 333, 585
MaeI CTAG 1 cut(s) 423
MaeII ACGT 3 cut(s) 21, 278, 297
MaeIII GTNAC 1 cut(s) 735
MalI GATC 1 cut(s) 262
MbiI CCGCTC 1 cut(s) 349
MboI GATC 1 cut(s) 260
MboII GAAGA 2 cut(s) 343, 668
MfeI CAATTG 2 cut(s) 207, 558
MflI RGATCY 1 cut(s) 260
MluCI AATT 9 cut(s) 43, 207, 414, 443, 451, 481, 558, 596, 672
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 1 cut(s) 25
Mph1103I ATGCAT 1 cut(s) 320
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 7 cut(s) 140, 219, 371, 417, 450, 590, 775
MunI CAATTG 2 cut(s) 207, 558
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 324
NcoI CCATGG 1 cut(s) 226
NdeII GATC 1 cut(s) 260
NlaIII CATG 6 cut(s) 9, 230, 322, 460, 633, 665
NlaIV GGNNCC 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 320
NspI RCATGY 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 356
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 1 cut(s) 71
Pfl23II CGTACG 1 cut(s) 294
PkrI GCNGC 4 cut(s) 329, 332, 348, 565
PmaCI CACGTG 1 cut(s) 22
PmlI CACGTG 1 cut(s) 22
Ppu21I YACGTR 2 cut(s) 22, 298
PshBI ATTAAT 1 cut(s) 450
PspCI CACGTG 1 cut(s) 22
PspLI CGTACG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 262
PsuI RGATCY 1 cut(s) 260
RsaI GTAC 4 cut(s) 296, 302, 516, 527
RsaNI GTAC 4 cut(s) 295, 301, 515, 526
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 7 cut(s) 140, 219, 371, 417, 450, 590, 775
SatI GCNGC 4 cut(s) 328, 331, 347, 564
Sau3AI GATC 1 cut(s) 260
SetI ASST 7 cut(s) 24, 253, 281, 300, 392, 554, 691
SfaNI GCATC 3 cut(s) 15, 333, 585
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 9 cut(s) 43, 207, 414, 443, 451, 481, 558, 596, 672
SsiI CCGC 2 cut(s) 347, 564
SspMI CTAG 1 cut(s) 423
StyI CCWWGG 2 cut(s) 226, 386
TaaI ACNGT 1 cut(s) 642
TaiI ACGT 3 cut(s) 24, 281, 300
TaqI TCGA 1 cut(s) 354
TasI AATT 9 cut(s) 43, 207, 414, 443, 451, 481, 558, 596, 672
TatI WGTACW 2 cut(s) 300, 525
TauI GCSGC 2 cut(s) 349, 566
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 7 cut(s) 140, 219, 371, 417, 450, 590, 775
Tru9I TTAA 7 cut(s) 140, 219, 371, 417, 450, 590, 775
TseI GCWGC 2 cut(s) 327, 330
TspDTI ATGAA 6 cut(s) 74, 335, 436, 445, 480, 615
TspGWI ACGGA 1 cut(s) 282
VspI ATTAAT 1 cut(s) 450
XapI RAATTY 2 cut(s) 596, 672
XceI RCATGY 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 1 cut(s) 423
Zsp2I ATGCAT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.