Rw5G015970

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000889
Physical Location & Seq
Forward (+)
19952007 .. 19952828
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015970.1

Sequence Viewer

Length: 489 bp
ATGGGTGATGAATTCAATGACCTACAACATGCTGGTATATGGACTCTTGTTCCAACATCCCCTTCCATGAATATCTTACCCAATAAATGGACAGAGCGGTATAAAGCACGATTAGTGGCAAATGGTTTCCATCAACGGGAAGGCGTTGATTATGGTGAGACCTTCAGTCCTGTTGTTAAGCATTCTACCATACGCCTTATTCTTGCCATTGCTGTCAATTTTGGATGGTCTGTTAAACAGTTAGATGTTCAAAATGCCTTTCTACATGGATTTCTTACTGAGGATGATCTAGGCAATCTTCATTATTTTTTGGGTATTGAAGTTCACCGTTCTTCTGCTGGTCTCTCTCTTACTCAATCTAAATATGCTCTTAATCTCTTACGCTGCACAGATATGATTGATGCCAAGCCCTTTGCCACTCCAGCTGTTAGTGGTAAACGTTTGAGTATCCATGATGGTGACAGCTTATCTGATCCTACCATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2

Protein Analysis

162

Amino Acids

18.04

Weight (kDa)

6.13

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 31 - 94 2.6e-20 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 87
AccBSI CCGCTC 1 cut(s) 97
AciI CCGC 1 cut(s) 97
AclI AACGTT 1 cut(s) 439
AclWI GGATC 1 cut(s) 467
AcsI RAATTY 1 cut(s) 11
AcuI CTGAAG 1 cut(s) 148
AfiI CCNNNNNNNGG 2 cut(s) 87, 136
AgsI TTSAA 3 cut(s) 16, 251, 320
AhdI GACNNNNNGTC 1 cut(s) 165
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
AluBI AGCT 2 cut(s) 425, 465
AluI AGCT 2 cut(s) 425, 465
Alw26I GTCTC 2 cut(s) 152, 347
AlwI GGATC 1 cut(s) 467
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 1 cut(s) 11
AsuHPI GGTGA 4 cut(s) 17, 167, 317, 470
BbvI GCAGC 1 cut(s) 371
BccI CCATC 3 cut(s) 138, 219, 449
BciVI GTATCC 1 cut(s) 458
BcoDI GTCTC 2 cut(s) 152, 347
BfaI CTAG 1 cut(s) 290
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 1 cut(s) 385
BlsI GCNGC 1 cut(s) 386
BmeRI GACNNNNNGTC 1 cut(s) 165
BmsI GCATC 1 cut(s) 391
BpmI CTGGAG 1 cut(s) 405
BsaI GGTCTC 2 cut(s) 152, 347
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 136
Bse3DI GCAATG 1 cut(s) 207
BseGI GGATG 3 cut(s) 56, 230, 289
BseLI CCNNNNNNNGG 2 cut(s) 87, 136
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 270
BseXI GCAGC 1 cut(s) 371
BsgI GTGCAG 1 cut(s) 370
BslI CCNNNNNNNGG 2 cut(s) 87, 136
BsmAI GTCTC 2 cut(s) 152, 347
BsmI GAATGC 1 cut(s) 181
Bso31I GGTCTC 2 cut(s) 152, 347
Bsp143I GATC 2 cut(s) 286, 472
BspACI CCGC 1 cut(s) 97
BspCNI CTCAG 1 cut(s) 271
BspPI GGATC 1 cut(s) 467
BspTNI GGTCTC 2 cut(s) 152, 347
BsrBI CCGCTC 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 207
BssMI GATC 2 cut(s) 286, 472
Bst4CI ACNGT 2 cut(s) 240, 329
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 56, 230, 289
BstKTI GATC 2 cut(s) 289, 475
BstMAI GTCTC 2 cut(s) 152, 347
BstMBI GATC 2 cut(s) 286, 472
BstMWI GCNNNNNNNGC 1 cut(s) 422
BstNSI RCATGY 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 371
BsuI GTATCC 1 cut(s) 458
BtsCI GGATG 3 cut(s) 56, 230, 289
CviAII CATG 4 cut(s) 29, 67, 266, 452
CviJI RGCY 3 cut(s) 409, 425, 465
CviKI_1 RGCY 3 cut(s) 409, 425, 465
DdeI CTNAG 1 cut(s) 279
DpnI GATC 2 cut(s) 288, 474
DpnII GATC 2 cut(s) 286, 472
DriI GACNNNNNGTC 1 cut(s) 165
Eam1105I GACNNNNNGTC 1 cut(s) 165
Eco31I GGTCTC 2 cut(s) 152, 347
Eco57I CTGAAG 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 11
FaeI CATG 4 cut(s) 32, 70, 269, 455
FatI CATG 4 cut(s) 28, 66, 265, 451
Fnu4HI GCNGC 1 cut(s) 385
FokI GGATG 3 cut(s) 43, 237, 296
Fsp4HI GCNGC 1 cut(s) 385
FspBI CTAG 1 cut(s) 290
GluI GCNGC 1 cut(s) 385
GsuI CTGGAG 1 cut(s) 405
Hin1II CATG 4 cut(s) 32, 70, 269, 455
HinfI GANTC 1 cut(s) 43
HphI GGTGA 4 cut(s) 17, 167, 317, 470
Hpy166II GTNNAC 2 cut(s) 325, 437
Hpy188I TCNGA 1 cut(s) 472
Hpy8I GTNNAC 2 cut(s) 325, 437
HpyAV CCTTC 3 cut(s) 72, 134, 172
HpyCH4III ACNGT 2 cut(s) 240, 329
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 1 cut(s) 387
HpyF10VI GCNNNNNNNGC 1 cut(s) 422
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 4 cut(s) 32, 70, 269, 455
Kzo9I GATC 2 cut(s) 286, 472
LpnPI CCDG 4 cut(s) 18, 183, 324, 435
Lsp1109I GCAGC 1 cut(s) 371
LweI GCATC 1 cut(s) 391
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 1 cut(s) 458
MalI GATC 2 cut(s) 288, 474
MbiI CCGCTC 1 cut(s) 97
MboI GATC 2 cut(s) 286, 472
MboII GAAGA 2 cut(s) 290, 324
MluCI AATT 2 cut(s) 11, 217
MlyI GAGTC 1 cut(s) 37
MmeI TCCRAC 1 cut(s) 77
MnlI CCTC 1 cut(s) 274
MseI TTAA 3 cut(s) 177, 234, 372
MslI CAYNNNNRTG 2 cut(s) 392, 456
MspA1I CMGCKG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 181
MwoI GCNNNNNNNGC 1 cut(s) 422
NdeII GATC 2 cut(s) 286, 472
NlaIII CATG 4 cut(s) 32, 70, 269, 455
NmuCI GTSAC 1 cut(s) 458
NspI RCATGY 1 cut(s) 32
PctI GAATGC 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 87
PkrI GCNGC 1 cut(s) 386
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
Psp1406I AACGTT 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 425
RseI CAYNNNNRTG 2 cut(s) 392, 456
SaqAI TTAA 3 cut(s) 177, 234, 372
SatI GCNGC 1 cut(s) 385
Sau3AI GATC 2 cut(s) 286, 472
SchI GAGTC 1 cut(s) 37
SetI ASST 5 cut(s) 24, 164, 427, 442, 467
SfaNI GCATC 1 cut(s) 391
SmiMI CAYNNNNRTG 2 cut(s) 392, 456
Sse9I AATT 2 cut(s) 11, 217
SsiI CCGC 1 cut(s) 97
SspMI CTAG 1 cut(s) 290
TaaI ACNGT 2 cut(s) 240, 329
TaiI ACGT 1 cut(s) 442
TasI AATT 2 cut(s) 11, 217
Tru1I TTAA 3 cut(s) 177, 234, 372
Tru9I TTAA 3 cut(s) 177, 234, 372
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 1 cut(s) 384
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 3 cut(s) 24, 83, 290
Van91I CCANNNNNTGG 1 cut(s) 87
XapI RAATTY 1 cut(s) 11
XceI RCATGY 1 cut(s) 32
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.