MD12G1175300.v1.1

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000889
Physical Location & Seq
Reverse (-)
25606277 .. 25608103
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1175300.v1.1.491

Sequence Viewer

Length: 1827 bp
ATGGTGTCCAGTTCAAGTCCCTCAAATCCCATTGCTTTTCCCTCTCTTCCAAATGCTTCCAACTTTCTGACTATCAAGTTGGATCGTACCAACTATCCCCTATGGCATGCCCAGATGTTGCCCTTGCTTCGTAGCAGGAATCTGGTGTCTTTTGTCGATGGAACCAACCCATATCCTCATGCCTTCTTGAAGGATGATGCTGGCCAACTCACTGATAAAGTCAATCCTGACTTTGATGCATGGATTCAGCAAGATGCTATGGTTCTCTCTTGGATCAACAGTTCGGTCCATCCCACAGTTCTTGCCTCTCTTATTGGTAAGACAAGTTCTATTTCTGCATGGACTTGTTTACGTGACAGGTATGCTTCTCAGTCTACTGGCCATCTCCTCCAATTGCGTAGTGAGCTTATGAATACCCATCGTGGTGATTCCTCTATTGCTGAGTTTCTTGATCGCATTAATTGTCTTGCCGACACTCTCTCCCTCTCTGGTTCTCCTATGACTGACTCTGATCTTGTTGCAATTATTTTAAATAATGTTGAGCCTGCTTATGAGAGTACTGTTGCTTCTGCACAAGCACGGGATGAGGCCATTACTTACAGTGCCTTGGAGGCTCTTTTTCTTGGTGCAGAGAGACATCAAAAGCTTCATACAGTGTTTGAAAATGGGCTCACTGCCCTTGCTGCTGGAAGAGGTCGTTCAGGACCTTCCTGTGGTCGCGGTGCTCCTTGCGGAGGCCGTGGTCACTTCAATAGTGGCCGTGGCCAGTTTCCTCCTCGTCCTCAAGGCCAGGAACACCATGCTCCTCCACACCGACAACCTTATGATGGCGTTTTTGGTGCTCCTCCTTCTCCAACCCAATCTGGTGGCCGTATCCAATGCCAAATATGTCGTCGTCACGGCCATTCTGCAATCGATTGTTTCAATCGCCTCAATATGGCCTATGAAGGACGTGTTCCTACTCCACGTCTCCAAGCTTATGTTGCTACTGCACCGTCTCCGGCAACTGCCTCTCCCTCTGGCATCCACAACTGGCTTTTTGACTCTGGAGCCAATGCACATATCACCAATGATTTGAATCAAGTTTCAAATCCTCGTCCTTACAATGGCACTGACAATGTTAATGGCGTGGTGGACGGATCAGGTTTGCACATTTCTCATGTTGGCACTTCTTTTATTCACATACCCCATCACTCATTTACCCTTCCCCACACCCTTCACTGCCCTAATGCTTCTACTAATGTTATTTCCATTAATCAATTTACCAAAGACAACCATTGTTCACTGACCTTATATCCCAATTCCTACCGTGTTCAGGACCTTCAAACGGGGAAGATGCTTTTGCAAGGCCGGAGTAAAAATGGTTTTTATCCATTTTTCAATTCTTCTGTGGTCAACCATGGAGTCTATGCATTTGTCGGTGTTAGAGTGTCAGATGCTATTTGGCATTCTAGGTTAGGTCACCCTGCAAGTTCTGTTTTGCGTAGTCTAATTTCCCAAAATAAATTACCTTTAACTGGTAATGCAACCTACGACTTTTGTTCATCATGTCCCCTTGGCAAAAGTCATAAGTTGCCTTTTAATTTATCTTCTTCCCAGTCATCGTTTCCTTTGGAATTAATACATTCAGATGTATGGATTTCTTCTTCTTATTCAATTAATGGTTACAAGTATTATGTTGTTTTTGTGGATGATTTTTCTTGTTATGCATGGCTCTATCCTTTAAAATTAAAGTCTGATGTTTTTCAAACCTTTGTTAATTTCAAAAAGCTTGCAGAAAATATGTTCAACACCAAAATTAAATTTTTTCAATCTGATGGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2

Protein Analysis

609

Amino Acids

66.71

Weight (kDa)

8.51

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 42 - 211 5.1e-16 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 345 - 424 5.4e-07 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 457 - 522 1.1e-09 GAG-pre-integrase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 374
AccII CGCG 1 cut(s) 720
AciI CCGC 2 cut(s) 720, 732
AclWI GGATC 3 cut(s) 90, 281, 1147
AcoI YGGCCR 6 cut(s) 202, 379, 757, 763, 868, 901
AcsI RAATTY 1 cut(s) 1802
AfaI GTAC 2 cut(s) 88, 559
AfiI CCNNNNNNNGG 8 cut(s) 713, 734, 827, 937, 1106, 1315, 1327, 1517
AflIII ACRYGT 1 cut(s) 952
AjiI CACGTC 2 cut(s) 953, 968
AjnI CCWGG 1 cut(s) 789
AloI GAACNNNNNNTCC 2 cut(s) 265, 297
AluBI AGCT 4 cut(s) 406, 646, 977, 1771
AluI AGCT 4 cut(s) 406, 646, 977, 1771
Alw21I GWGCWC 2 cut(s) 727, 844
Alw26I GTCTC 3 cut(s) 628, 974, 1002
AlwI GGATC 3 cut(s) 90, 281, 1147
ApeKI GCWGC 1 cut(s) 683
ApoI RAATTY 1 cut(s) 1802
ArsI GACNNNNNNTTYG 2 cut(s) 877, 909
AseI ATTAAT 4 cut(s) 459, 1254, 1619, 1659
AspS9I GGNCC 3 cut(s) 286, 704, 1318
AsuHPI GGTGA 3 cut(s) 437, 1057, 1454
AvaII GGWCC 3 cut(s) 286, 704, 1318
BalI TGGCCA 3 cut(s) 204, 381, 765
BanII GRGCYC 1 cut(s) 672
BarI GAAGNNNNNNTAC 2 cut(s) 550, 582
Bbv12I GWGCWC 2 cut(s) 727, 844
BbvI GCAGC 1 cut(s) 670
BccI CCATC 7 cut(s) 152, 297, 390, 426, 821, 1197, 1811
BceAI ACGGC 4 cut(s) 723, 744, 855, 916
BciT130I CCWGG 1 cut(s) 791
BciVI GTATCC 1 cut(s) 884
BcoDI GTCTC 3 cut(s) 628, 974, 1002
BfaI CTAG 1 cut(s) 1452
BfuI GTATCC 1 cut(s) 884
BglI GCCNNNNNGGC 1 cut(s) 611
BisI GCNGC 1 cut(s) 684
BlsI GCNGC 1 cut(s) 685
BmcAI AGTACT 1 cut(s) 559
Bme1390I CCNGG 1 cut(s) 791
Bme18I GGWCC 3 cut(s) 286, 704, 1318
BmgBI CACGTC 2 cut(s) 953, 968
BmgT120I GGNCC 3 cut(s) 286, 704, 1318
BmiI GGNNCC 2 cut(s) 163, 1051
BmrFI CCNGG 1 cut(s) 791
BmrI ACTGGG 1 cut(s) 1591
BmsI GCATC 6 cut(s) 187, 226, 244, 1032, 1326, 1426
BmuI ACTGGG 1 cut(s) 1591
BpmI CTGGAG 1 cut(s) 1068
BpuEI CTTGAG 1 cut(s) 768
Bsa29I ATCGAT 1 cut(s) 915
BsaAI YACGTR 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 1077
BsaJI CCNNGG 5 cut(s) 606, 739, 760, 1399, 1555
BsaXI ACNNNNNCTCC 4 cut(s) 464, 494, 997, 1027
Bsc4I CCNNNNNNNGG 8 cut(s) 713, 734, 827, 937, 1106, 1315, 1327, 1517
Bse1I ACTGG 6 cut(s) 9, 382, 766, 1037, 1522, 1597
Bse3DI GCAATG 1 cut(s) 30
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 1 cut(s) 791
BseCI ATCGAT 1 cut(s) 915
BseDI CCNNGG 5 cut(s) 606, 739, 760, 1399, 1555
BseGI GGATG 5 cut(s) 199, 289, 589, 1023, 1696
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 8 cut(s) 713, 734, 827, 937, 1106, 1315, 1327, 1517
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 2 cut(s) 383, 432
BseNI ACTGG 6 cut(s) 9, 382, 766, 1037, 1522, 1597
BseRI GAGGAG 4 cut(s) 377, 765, 795, 834
BseXI GCAGC 1 cut(s) 670
BsgI GTGCAG 3 cut(s) 555, 648, 975
Bsh1236I CGCG 1 cut(s) 720
BshVI ATCGAT 1 cut(s) 915
BsiHKAI GWGCWC 2 cut(s) 727, 844
BsiSI CCGG 2 cut(s) 1001, 1351
BslFI GGGAC 2 cut(s) 3, 1536
BslI CCNNNNNNNGG 8 cut(s) 713, 734, 827, 937, 1106, 1315, 1327, 1517
BsmAI GTCTC 3 cut(s) 628, 974, 1002
BsmBI CGTCTC 2 cut(s) 974, 1002
BsmFI GGGAC 2 cut(s) 3, 1536
BsmI GAATGC 1 cut(s) 1447
Bsp1286I GDGCHC 3 cut(s) 672, 727, 844
Bsp143I GATC 5 cut(s) 82, 273, 451, 511, 1139
Bsp19I CCATGG 1 cut(s) 1399
BspACI CCGC 2 cut(s) 720, 732
BspCNI CTCAG 2 cut(s) 382, 433
BspDI ATCGAT 1 cut(s) 915
BspFNI CGCG 1 cut(s) 720
BspLI GGNNCC 2 cut(s) 163, 1051
BspPI GGATC 3 cut(s) 90, 281, 1147
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 6 cut(s) 9, 382, 766, 1037, 1522, 1597
BssECI CCNNGG 5 cut(s) 606, 739, 760, 1399, 1555
BssMI GATC 5 cut(s) 82, 273, 451, 511, 1139
BssT1I CCWWGG 3 cut(s) 606, 1399, 1555
Bst2UI CCWGG 1 cut(s) 791
Bst4CI ACNGT 7 cut(s) 281, 298, 562, 602, 655, 996, 1310
Bst6I CTCTTC 2 cut(s) 51, 685
BstBAI YACGTR 1 cut(s) 353
BstC8I GCNNGC 4 cut(s) 108, 202, 546, 1773
BstDEI CTNAG 2 cut(s) 369, 441
BstDSI CCRYGG 3 cut(s) 739, 760, 1399
BstEII GGTNACC 1 cut(s) 1460
BstENI CCTNNNNNAGG 1 cut(s) 732
BstF5I GGATG 5 cut(s) 199, 289, 589, 1023, 1696
BstFNI CGCG 1 cut(s) 720
BstKTI GATC 5 cut(s) 85, 276, 454, 514, 1142
BstMAI GTCTC 3 cut(s) 628, 974, 1002
BstMBI GATC 5 cut(s) 82, 273, 451, 511, 1139
BstMWI GCNNNNNNNGC 4 cut(s) 403, 611, 683, 983
BstNI CCWGG 1 cut(s) 791
BstNSI RCATGY 1 cut(s) 110
BstPI GGTNACC 1 cut(s) 1460
BstSCI CCNGG 1 cut(s) 789
BstUI CGCG 1 cut(s) 720
BstV1I GCAGC 1 cut(s) 670
BstXI CCANNNNNNTGG 1 cut(s) 866
Bsu15I ATCGAT 1 cut(s) 915
BsuI GTATCC 1 cut(s) 884
BsuTUI ATCGAT 1 cut(s) 915
BtgI CCRYGG 3 cut(s) 739, 760, 1399
BtrI CACGTC 2 cut(s) 953, 968
BtsCI GGATG 5 cut(s) 199, 289, 589, 1023, 1696
BtsI GCAGTG 2 cut(s) 672, 1219
BtsIMutI CAGTG 7 cut(s) 210, 607, 660, 672, 1110, 1219, 1283
Cac8I GCNNGC 4 cut(s) 108, 202, 546, 1773
Cfr13I GGNCC 3 cut(s) 286, 704, 1318
ClaI ATCGAT 1 cut(s) 915
Csp6I GTAC 2 cut(s) 87, 558
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 9 cut(s) 107, 179, 240, 339, 800, 1160, 1400, 1548, 1710
CviQI GTAC 2 cut(s) 87, 558
DdeI CTNAG 2 cut(s) 369, 441
DpnI GATC 5 cut(s) 84, 275, 453, 513, 1141
DpnII GATC 5 cut(s) 82, 273, 451, 511, 1139
DraI TTTAAA 2 cut(s) 531, 1725
EaeI YGGCCR 6 cut(s) 202, 379, 757, 763, 868, 901
Eam1104I CTCTTC 2 cut(s) 51, 685
EarI CTCTTC 2 cut(s) 51, 685
Eco130I CCWWGG 3 cut(s) 606, 1399, 1555
Eco24I GRGCYC 1 cut(s) 672
Eco47I GGWCC 3 cut(s) 286, 704, 1318
Eco91I GGTNACC 1 cut(s) 1460
EcoNI CCTNNNNNAGG 1 cut(s) 732
EcoO109I RGGNCCY 2 cut(s) 704, 1318
EcoO65I GGTNACC 1 cut(s) 1460
EcoRII CCWGG 1 cut(s) 789
EcoT14I CCWWGG 3 cut(s) 606, 1399, 1555
EcoT22I ATGCAT 3 cut(s) 241, 1414, 1711
EcoT38I GRGCYC 1 cut(s) 672
ErhI CCWWGG 3 cut(s) 606, 1399, 1555
Esp3I CGTCTC 2 cut(s) 974, 1002
FaeI CATG 9 cut(s) 110, 182, 243, 342, 803, 1163, 1403, 1551, 1713
FaqI GGGAC 2 cut(s) 3, 1536
FatI CATG 9 cut(s) 106, 178, 239, 338, 799, 1159, 1399, 1547, 1709
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 1 cut(s) 684
FokI GGATG 5 cut(s) 206, 276, 596, 1010, 1703
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 1 cut(s) 684
FspBI CTAG 1 cut(s) 1452
GluI GCNGC 1 cut(s) 684
GsuI CTGGAG 1 cut(s) 1068
HapII CCGG 2 cut(s) 1001, 1351
Hin1II CATG 9 cut(s) 110, 182, 243, 342, 803, 1163, 1403, 1551, 1713
HincII GTYRAC 1 cut(s) 1396
HindII GTYRAC 1 cut(s) 1396
HindIII AAGCTT 3 cut(s) 644, 975, 1769
HinfI GANTC 7 cut(s) 139, 244, 428, 506, 1043, 1078, 1404
HpaII CCGG 2 cut(s) 1001, 1351
HphI GGTGA 3 cut(s) 437, 1057, 1454
Hpy166II GTNNAC 5 cut(s) 350, 375, 1135, 1283, 1396
Hpy188I TCNGA 6 cut(s) 69, 511, 1435, 1630, 1738, 1816
Hpy188III TCNNGA 6 cut(s) 187, 227, 449, 702, 1047, 1316
Hpy8I GTNNAC 5 cut(s) 350, 375, 1135, 1283, 1396
Hpy99I CGWCG 1 cut(s) 897
HpyAV CCTTC 8 cut(s) 184, 193, 717, 858, 941, 1214, 1226, 1331
HpyCH4III ACNGT 7 cut(s) 281, 298, 562, 602, 655, 996, 1310
HpyCH4IV ACGT 3 cut(s) 352, 952, 967
HpyF10VI GCNNNNNNNGC 4 cut(s) 403, 611, 683, 983
HpyF3I CTNAG 2 cut(s) 369, 441
HpySE526I ACGT 3 cut(s) 352, 952, 967
Hsp92II CATG 9 cut(s) 110, 182, 243, 342, 803, 1163, 1403, 1551, 1713
Kzo9I GATC 5 cut(s) 82, 273, 451, 511, 1139
LmnI GCTCC 4 cut(s) 730, 808, 847, 1049
Lsp1109I GCAGC 1 cut(s) 670
LweI GCATC 6 cut(s) 187, 226, 244, 1032, 1326, 1426
MaeI CTAG 1 cut(s) 1452
MaeII ACGT 3 cut(s) 352, 952, 967
MaeIII GTNAC 5 cut(s) 353, 743, 896, 1460, 1664
MalI GATC 5 cut(s) 84, 275, 453, 513, 1141
MboI GATC 5 cut(s) 82, 273, 451, 511, 1139
MboII GAAGA 8 cut(s) 38, 702, 1345, 1377, 1581, 1584, 1635, 1638
MfeI CAATTG 1 cut(s) 392
MhlI GDGCHC 3 cut(s) 672, 727, 844
MlsI TGGCCA 3 cut(s) 204, 381, 765
MluNI TGGCCA 3 cut(s) 204, 381, 765
MlyI GAGTC 3 cut(s) 500, 1037, 1413
MmeI TCCRAC 3 cut(s) 60, 84, 878
Mox20I TGGCCA 3 cut(s) 204, 381, 765
Mph1103I ATGCAT 3 cut(s) 241, 1414, 1711
MscI TGGCCA 3 cut(s) 204, 381, 765
MslI CAYNNNNRTG 2 cut(s) 423, 1629
Msp20I TGGCCA 3 cut(s) 204, 381, 765
MspI CCGG 2 cut(s) 1001, 1351
MspR9I CCNGG 1 cut(s) 791
MunI CAATTG 1 cut(s) 392
Mva1269I GAATGC 1 cut(s) 1447
MvaI CCWGG 1 cut(s) 791
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 4 cut(s) 403, 611, 683, 983
NcoI CCATGG 1 cut(s) 1399
NdeII GATC 5 cut(s) 82, 273, 451, 511, 1139
NlaIII CATG 9 cut(s) 110, 182, 243, 342, 803, 1163, 1403, 1551, 1713
NlaIV GGNNCC 2 cut(s) 163, 1051
NmuCI GTSAC 4 cut(s) 353, 743, 896, 1460
NsiI ATGCAT 3 cut(s) 241, 1414, 1711
NspI RCATGY 1 cut(s) 110
PaeI GCATGC 1 cut(s) 110
PctI GAATGC 1 cut(s) 1447
PfeI GAWTC 4 cut(s) 139, 244, 428, 1078
PkrI GCNGC 1 cut(s) 685
PleI GAGTC 3 cut(s) 500, 1037, 1412
PpsI GAGTC 3 cut(s) 500, 1037, 1412
Ppu21I YACGTR 1 cut(s) 353
PpuMI RGGWCCY 2 cut(s) 704, 1318
PshBI ATTAAT 4 cut(s) 459, 1254, 1619, 1659
Psp5II RGGWCCY 2 cut(s) 704, 1318
Psp6I CCWGG 1 cut(s) 789
PspEI GGTNACC 1 cut(s) 1460
PspGI CCWGG 1 cut(s) 789
PspN4I GGNNCC 2 cut(s) 163, 1051
PspPI GGNCC 3 cut(s) 286, 704, 1318
PspPPI RGGWCCY 2 cut(s) 704, 1318
PsrI GAACNNNNNNTAC 2 cut(s) 310, 342
RsaI GTAC 2 cut(s) 88, 559
RsaNI GTAC 2 cut(s) 87, 558
RseI CAYNNNNRTG 2 cut(s) 423, 1629
SatI GCNGC 1 cut(s) 684
Sau3AI GATC 5 cut(s) 82, 273, 451, 511, 1139
Sau96I GGNCC 3 cut(s) 286, 704, 1318
ScaI AGTACT 1 cut(s) 559
SchI GAGTC 3 cut(s) 500, 1037, 1413
ScrFI CCNGG 1 cut(s) 791
SduI GDGCHC 3 cut(s) 672, 727, 844
SfaNI GCATC 6 cut(s) 187, 226, 244, 1032, 1326, 1426
SinI GGWCC 3 cut(s) 286, 704, 1318
SmiMI CAYNNNNRTG 2 cut(s) 423, 1629
SmlI CTYRAG 1 cut(s) 783
SmoI CTYRAG 1 cut(s) 783
SphI GCATGC 1 cut(s) 110
SsiI CCGC 2 cut(s) 720, 732
SspMI CTAG 1 cut(s) 1452
StyD4I CCNGG 1 cut(s) 789
StyI CCWWGG 3 cut(s) 606, 1399, 1555
TaaI ACNGT 7 cut(s) 281, 298, 562, 602, 655, 996, 1310
TaiI ACGT 3 cut(s) 355, 955, 970
TaqI TCGA 2 cut(s) 156, 915
TaqII GACCGA 1 cut(s) 274
TatI WGTACW 1 cut(s) 557
TfiI GAWTC 4 cut(s) 139, 244, 428, 1078
TscAI CASTG 7 cut(s) 217, 607, 660, 679, 1117, 1226, 1290
TseFI GTSAC 4 cut(s) 353, 743, 896, 1460
TseI GCWGC 1 cut(s) 683
Tsp45I GTSAC 4 cut(s) 353, 743, 896, 1460
TspDTI ATGAA 4 cut(s) 425, 638, 960, 1533
TspGWI ACGGA 1 cut(s) 1152
TspRI CASTG 7 cut(s) 217, 607, 660, 679, 1117, 1226, 1290
VpaK11BI GGWCC 3 cut(s) 286, 704, 1318
VspI ATTAAT 4 cut(s) 459, 1254, 1619, 1659
XagI CCTNNNNNAGG 1 cut(s) 732
XapI RAATTY 1 cut(s) 1802
XceI RCATGY 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 374
XspI CTAG 1 cut(s) 1452
ZrmI AGTACT 1 cut(s) 559
Zsp2I ATGCAT 3 cut(s) 241, 1414, 1711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.