pycom03g07840

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000889
Physical Location & Seq
Forward (+)
6220014 .. 6220454
441 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g07840.1

Sequence Viewer

Length: 441 bp
ATGCCTATCACCAATTGTATCCTATTGTCTCTTGCAGCTCAATTTCACTGGTTTTTAAACCAATTGGACATAAATAATGCTTTCTTGCATGAGGATTTACAAGAAGATGTCTTTATGCAGCAGCCTCCAGGGTTTAGTGATCCCTCATTTCCAAACCATGTTTGCAAACTCAAGAAGTCACTCTATGGCCTTAAACAGGCTCCACGAGCTTGGTTTGACAAATTATTCCAAGTGTTGCATTCCTTTGGTTTTCAGCAATCCTCTTTTGATGTTTCTCTCTTTGTCTTAAAAGCTCCAACCTTGGTTATAATCCTTGTATATGTTGATGACATTCTAGTCACGGGTCCTAATGCTACTCTCTGTAAACATTACATTCAGAAATTGAGTACTGTCTTTCTAGTCAAAGATTTCGGTCCTTTGCACTATTTTTTTGGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

147

Amino Acids

16.73

Weight (kDa)

6.56

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 7 - 145 3.3e-37 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 308
AasI GACNNNNNNGTC 1 cut(s) 335
AclWI GGATC 1 cut(s) 134
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 196
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 3 cut(s) 38, 209, 293
AluI AGCT 3 cut(s) 38, 209, 293
Alw26I GTCTC 1 cut(s) 33
AlwI GGATC 1 cut(s) 134
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 3 cut(s) 35, 118, 121
AspS9I GGNCC 2 cut(s) 344, 413
AvaII GGWCC 2 cut(s) 344, 413
BauI CACGAG 1 cut(s) 204
BbvI GCAGC 3 cut(s) 47, 130, 133
BciT130I CCWGG 1 cut(s) 129
BciVI GTATCC 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 33
BfaI CTAG 2 cut(s) 335, 398
BfuI GTATCC 1 cut(s) 29
BisI GCNGC 3 cut(s) 36, 119, 122
BlsI GCNGC 3 cut(s) 37, 120, 123
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 2 cut(s) 344, 413
BmgT120I GGNCC 2 cut(s) 344, 413
BmiI GGNNCC 2 cut(s) 201, 345
BmrFI CCNGG 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 128, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 53
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 2 cut(s) 128, 300
BseLI CCNNNNNNNGG 1 cut(s) 196
BseNI ACTGG 1 cut(s) 53
BseXI GCAGC 3 cut(s) 47, 130, 133
BshFI GGCC 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 33
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 189
Bsp143I GATC 1 cut(s) 139
BspANI GGCC 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 201, 345
BspPI GGATC 1 cut(s) 134
BsrI ACTGG 1 cut(s) 53
BssECI CCNNGG 2 cut(s) 128, 300
BssMI GATC 1 cut(s) 139
BssSI CACGAG 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 391
BstENI CCTNNNNNAGG 1 cut(s) 194
BstKTI GATC 1 cut(s) 142
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 127
BstV1I GCAGC 3 cut(s) 47, 130, 133
BstXI CCANNNNNNTGG 1 cut(s) 210
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 46
Cfr13I GGNCC 2 cut(s) 344, 413
Csp6I GTAC 1 cut(s) 387
CviAII CATG 2 cut(s) 89, 158
CviJI RGCY 6 cut(s) 38, 124, 189, 200, 209, 293
CviKI_1 RGCY 6 cut(s) 38, 124, 189, 200, 209, 293
CviQI GTAC 1 cut(s) 387
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
DraI TTTAAA 1 cut(s) 57
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 344, 413
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 92, 161
FaiI YATR 8 cut(s) 71, 90, 116, 159, 186, 308, 319, 321
FatI CATG 2 cut(s) 88, 157
Fnu4HI GCNGC 3 cut(s) 36, 119, 122
Fsp4HI GCNGC 3 cut(s) 36, 119, 122
FspBI CTAG 2 cut(s) 335, 398
GluI GCNGC 3 cut(s) 36, 119, 122
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 1 cut(s) 189
Hin1II CATG 2 cut(s) 92, 161
Hpy166II GTNNAC 1 cut(s) 365
Hpy188I TCNGA 1 cut(s) 378
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 1 cut(s) 365
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 6 cut(s) 35, 88, 118, 165, 238, 421
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
Hsp92II CATG 2 cut(s) 92, 161
Kzo9I GATC 1 cut(s) 139
LmnI GCTCC 2 cut(s) 205, 298
LpnPI CCDG 4 cut(s) 34, 114, 141, 182
Lsp1109I GCAGC 3 cut(s) 47, 130, 133
MaeI CTAG 2 cut(s) 335, 398
MaeIII GTNAC 2 cut(s) 177, 337
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 1 cut(s) 116
MfeI CAATTG 2 cut(s) 13, 62
MluCI AATT 5 cut(s) 13, 41, 62, 221, 380
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 4 cut(s) 85, 135, 154, 271
MseI TTAA 3 cut(s) 56, 192, 287
MspR9I CCNGG 1 cut(s) 129
MunI CAATTG 2 cut(s) 13, 62
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 1 cut(s) 206
NdeII GATC 1 cut(s) 139
NlaIII CATG 2 cut(s) 92, 161
NlaIV GGNNCC 2 cut(s) 201, 345
NmuCI GTSAC 2 cut(s) 177, 337
PctI GAATGC 1 cut(s) 238
PkrI GCNGC 3 cut(s) 37, 120, 123
PpuMI RGGWCCY 1 cut(s) 344
PsiI TTATAA 1 cut(s) 308
Psp5II RGGWCCY 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 2 cut(s) 201, 345
PspPI GGNCC 2 cut(s) 344, 413
PspPPI RGGWCCY 1 cut(s) 344
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SaqAI TTAA 3 cut(s) 56, 192, 287
SatI GCNGC 3 cut(s) 36, 119, 122
Sau3AI GATC 1 cut(s) 139
Sau96I GGNCC 2 cut(s) 344, 413
ScaI AGTACT 1 cut(s) 388
ScrFI CCNGG 1 cut(s) 129
SetI ASST 4 cut(s) 40, 211, 295, 302
SinI GGWCC 2 cut(s) 344, 413
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 5 cut(s) 13, 41, 62, 221, 380
SspMI CTAG 2 cut(s) 335, 398
StyD4I CCNGG 1 cut(s) 127
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 391
TaqII GACCGA 1 cut(s) 401
TasI AATT 5 cut(s) 13, 41, 62, 221, 380
TatI WGTACW 1 cut(s) 386
Tru1I TTAA 3 cut(s) 56, 192, 287
Tru9I TTAA 3 cut(s) 56, 192, 287
TscAI CASTG 1 cut(s) 53
TseFI GTSAC 2 cut(s) 177, 337
TseI GCWGC 3 cut(s) 35, 118, 121
Tsp45I GTSAC 2 cut(s) 177, 337
TspRI CASTG 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 344, 413
XagI CCTNNNNNAGG 1 cut(s) 194
XspI CTAG 2 cut(s) 335, 398
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.