MD05G1050400.v1.1

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000889
Physical Location & Seq
Forward (+)
8735903 .. 8740360
4458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1050400.v1.1.491

Sequence Viewer

Length: 2478 bp
ATGAGGAACATGATAAACCTAGTTCGGATAAATCTCCAAACCCTAACGACCAGTCCACGTCAGCACCATCAAACCGTCCCGCTGCAATCTGCAACCGAAAGGATTCCACCAGCTGTACAACCTGGACAAAAGGAATGTTGCACATGGATACAACATTCCTTGACAGCTAGGTTACCACATGGTTTCTGCACCTATGTTCTAGTTGCTGCATCATGGGCAGCAAGGACACCACTTGTATTGGATTTGTTTATTGCATGCGTAGCATTGGAAGCTCTTCTTCTTGGTGCAGAGAGACGTCAAAAGCTTCATACTGTGTTTGAAAATGGGCTCACTGCCCTTGCTGCTGGAAGAGGTCGTTCAGGACATTCCCGTGGTCGCGGTGCTCCTCGCGGAGGCCGTGGTCATTTCAATGGTGGCCGTGGCCAGTTTCCTCCTCGTCCTCAAGGCCAGGAACACCATGCTCCTCCACCCCGACAACCTCATGATGGCGTTTTTGGTGCTCCTCCTTCTCCAACCCAATCTGGTGGCCGTATCCAATGCCAAATATGTCGTCGTCATGGCCATTCTGCAATCGATTGTTTCAATCGCCTCAATATGGCCTATGAAGAACGTGTTCCTGCTCCACATCTCCAAGCTTATGCTGCTGCTGCACCATCTCCGGCATCTGCCTCTCCCTCTGGCACCCAAAACTGGCTTTTTGACTCTGGAGCCAATGCACATATCACCAATGATTTGAATCAAGTTTCAAATCCTCGTCCCTACAATGGCACTGACAATGTTAATGGCGTGGTGGATGGATTAGGTTTGCACATTTCTCATGTTGGCACTTCTTTTATTCACACACCCCATCACTCATTTACCCTTCCCAACACCCTTCACTGCCCTAATGCTTCTACTAATGTTATTTCCATCAATCAATTTACCAAAGACAACCATTGTTCACTGACCTTATATCCCAATTCCTACCGTGGTCAGGACCTCCAAACGAGGAAGATGCTTTTGCAAGGCCGGAGTAAAAATGGTTTTTATCCATTTTCCAGTTCTTCTGTGGTCAACCATGGAGTCTCTGCATTTGTCGGTGTTAGAGTGTCGGATGCTATTTGGCATTCTAGGTTAGGGAACAATAATGCTCACTTGTCTCAGTTTATCACGCAGCTTGGTACTCATTTTGCAATGAAAGATCTTGGGCCCTTACACTACTTCTTGGGGATAGAGGTTTCCCGGACGCCTTCTGCTTTCTATCTCACTCAATCCAAGTATATTTTGGAGCTTCTACAGAAAACTAATATGGCTGACGCCAAGCCCATCTCAACGCCAGTTCCCAGTGGTAAACGCTTGAGTTTATATGATGGTGAGCCTCTCTTAGATGGATCTTCTTTTCGCAGTGTTGTGGGCGCCCTTCAATACCTTATATTTACACGTCCTGATATTGCTTATGCTGTCAACCAAGTGTGTCAGTTCATGCACTCTCCAACTACTGCGCATTGGGCTGCAGTCAAACGCATCTTACGGTATTTGAAAGGCACACATGCTCACGGCTTACTTTACAAACCCAGTCCCCTCACTCTAACTGCCTTCGCTGACGCTGACTACGCTGGCGACCCTGATAACCGTCGTTCCACTGGCGGTCACTGCATATTTCTTGGCAATAATTTAATTTCATGGAGTTCGAAAAAGCAACGTGGAGTGTCACGATCTAGCACAGAGGCGGAGTATCGTCAACTTGCCTATACTTCTGCCCATCTCTCATGGTTTCACAATCTATTCCGTGATTTACATCTTCCCTTACAGCCACCTCGTCTCTGGTGTGACAATATCAGTGCCATTGCTGTTGCTTCCAATCCCGTTTATCAAGCTCGCATGCGCCATGTTGAAGTGGATTATCACTACATCCGGGACAAGGTCATTCGCAAGGAAATAGAAGTTGGTTATGTAGCTACCACAGACCAAATTGCCGATTTTTTAACCAAGGGTTTGTCCTCTGTCCGCTTCCATTATCTTATCTCCAAGCTTCCTGTTAGTCAACGCCCCGTCAGCTTGCGGGGGCGTGATAAACCTAGTTCGGATAAATCTCCAAACCCTAACGACCGGTCCACGTCAGCACCATCAAACCTTCCTGCTGCAATCTGCAACCGAAAGGATTCCACTAGCTGTACAACCTGGACAAAAGGAATATGGCAGCCAAAACAAGACACGCGTGGGGATCAAGATGGATTGAAAGGAACATATCGAGTAGATGTTGATATTCCAACCAATGATTGGGCCAACATTTCTGATTTTTATGATGTCCTAGTTTTCAATATAGGGCACTGGATGCCGTTTAGAGCAGATGCCCATCCAGCAATTTGGTTGGGACAAAAGGATGTCGTTTTAGTCTGGGGCCAGGATTGCATGCATTGGTGCTTACCTGGTGTACCAGATACATGGGTTGACATATTATCAAAACTGATTCACGTTGGGTTGGAGATAAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2

Protein Analysis

826

Amino Acids

91.58

Weight (kDa)

9.4

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 231 - 310 1.8e-06 Pol polyprotein, beta-barrel domain
RVT_2 PF07727 359 - 440 1.2e-10 Reverse transcriptase (RNA-dependent DNA polymerase)
PC-Esterase PF13839 773 - 815 3.8e-13 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 298
Acc16I TGCGCA 1 cut(s) 1482
AccB1I GGYRCC 2 cut(s) 680, 1394
AccB7I CCANNNNNTGG 1 cut(s) 2259
AccII CGCG 3 cut(s) 378, 390, 2197
AciI CCGC 7 cut(s) 80, 378, 390, 1626, 1709, 1987, 2041
AclWI GGATC 2 cut(s) 1378, 2211
AcoI YGGCCR 4 cut(s) 415, 421, 526, 559
AcyI GRCGYC 4 cut(s) 295, 1226, 1296, 1395
AfaI GTAC 4 cut(s) 117, 1162, 2155, 2415
AfiI CCNNNNNNNGG 8 cut(s) 392, 485, 595, 690, 764, 973, 1900, 2259
AflIII ACRYGT 3 cut(s) 610, 1418, 2195
AgeI ACCGGT 1 cut(s) 2088
AjiI CACGTC 3 cut(s) 59, 1421, 2097
AjnI CCWGG 5 cut(s) 121, 447, 2159, 2382, 2407
AjuI GAANNNNNNNTTGG 2 cut(s) 1908, 1940
Alw21I GWGCWC 2 cut(s) 385, 502
Alw26I GTCTC 4 cut(s) 286, 1069, 1143, 1805
AlwI GGATC 2 cut(s) 1378, 2211
ApaI GGGCCC 1 cut(s) 1191
ArsI GACNNNNNNTTYG 2 cut(s) 535, 567
AsiGI ACCGGT 1 cut(s) 2088
Asp700I GAANNNNTTC 4 cut(s) 102, 273, 612, 2140
AspLEI GCGC 3 cut(s) 1397, 1483, 1866
AspS9I GGNCC 6 cut(s) 976, 1187, 1188, 2091, 2262, 2380
AsuC2I CCSGG 2 cut(s) 1222, 1895
AsuHPI GGTGA 2 cut(s) 715, 1364
AsuII TTCGAA 1 cut(s) 1670
AvaII GGWCC 2 cut(s) 976, 2091
BaeGI GKGCMC 2 cut(s) 1191, 2310
BaeI ACNNNNGTAYC 2 cut(s) 2412, 2445
BalI TGGCCA 2 cut(s) 423, 561
BanI GGYRCC 2 cut(s) 680, 1394
BanII GRGCYC 2 cut(s) 330, 1191
Bbv12I GWGCWC 2 cut(s) 385, 502
BceAI ACGGC 5 cut(s) 381, 402, 513, 1552, 2302
BcgI CGANNNNNNTGC 2 cut(s) 976, 1010
BciT130I CCWGG 5 cut(s) 123, 449, 2161, 2384, 2409
BciVI GTATCC 2 cut(s) 141, 542
BcnI CCSGG 2 cut(s) 1222, 1895
BcoDI GTCTC 4 cut(s) 286, 1069, 1143, 1805
BfaI CTAG 8 cut(s) 20, 168, 200, 1110, 1698, 2058, 2148, 2291
BfmI CTRYAG 2 cut(s) 1274, 1491
BfoI RGCGCY 1 cut(s) 1398
BfuI GTATCC 2 cut(s) 141, 542
BglII AGATCT 1 cut(s) 1180
Bme1390I CCNGG 7 cut(s) 123, 449, 1222, 1895, 2161, 2384, 2409
Bme18I GGWCC 2 cut(s) 976, 2091
BmgBI CACGTC 3 cut(s) 59, 1421, 2097
BmgT120I GGNCC 6 cut(s) 976, 1187, 1188, 2091, 2262, 2380
BmiI GGNNCC 5 cut(s) 682, 709, 1189, 1396, 2381
BmrFI CCNGG 7 cut(s) 123, 449, 1222, 1895, 2161, 2384, 2409
BmrI ACTGGG 2 cut(s) 1317, 1548
BmsI GCATC 7 cut(s) 218, 671, 984, 1084, 1512, 2304, 2320
BmuI ACTGGG 2 cut(s) 1317, 1548
BpmI CTGGAG 1 cut(s) 726
Bpu14I TTCGAA 1 cut(s) 1670
BpuEI CTTGAG 2 cut(s) 426, 1357
BpuMI CCSGG 2 cut(s) 1222, 1895
Bsa29I ATCGAT 1 cut(s) 573
BsaBI GATNNNNATC 3 cut(s) 735, 1776, 2334
BsaHI GRCGYC 4 cut(s) 295, 1226, 1296, 1395
BsaJI CCNNGG 6 cut(s) 370, 397, 418, 967, 1057, 1968
BsaWI WCCGGW 1 cut(s) 2088
Bsc4I CCNNNNNNNGG 8 cut(s) 392, 485, 595, 690, 764, 973, 1900, 2259
Bse118I RCCGGY 1 cut(s) 2088
Bse1I ACTGG 9 cut(s) 51, 424, 695, 1038, 1316, 1323, 1554, 1627, 2315
Bse3DI GCAATG 2 cut(s) 1179, 1824
Bse8I GATNNNNATC 3 cut(s) 735, 1776, 2334
BseBI CCWGG 5 cut(s) 123, 449, 2161, 2384, 2409
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 6 cut(s) 370, 397, 418, 967, 1057, 1968
BseGI GGATG 6 cut(s) 799, 1099, 1890, 2319, 2335, 2368
BseJI GATNNNNATC 3 cut(s) 735, 1776, 2334
BseLI CCNNNNNNNGG 8 cut(s) 392, 485, 595, 690, 764, 973, 1900, 2259
BseMI GCAATG 2 cut(s) 1179, 1824
BseMII CTCAG 1 cut(s) 1154
BseNI ACTGG 9 cut(s) 51, 424, 695, 1038, 1316, 1323, 1554, 1627, 2315
BseRI GAGGAG 4 cut(s) 375, 423, 453, 492
BseSI GKGCMC 2 cut(s) 1191, 2310
BsgI GTGCAG 3 cut(s) 172, 306, 633
Bsh1236I CGCG 3 cut(s) 378, 390, 2197
Bsh1285I CGRYCG 1 cut(s) 2089
BshNI GGYRCC 2 cut(s) 680, 1394
BshTI ACCGGT 1 cut(s) 2088
BshVI ATCGAT 1 cut(s) 573
BsiEI CGRYCG 1 cut(s) 2089
BsiHKAI GWGCWC 2 cut(s) 385, 502
BsiSI CCGG 5 cut(s) 659, 1009, 1222, 1894, 2089
BslFI GGGAC 5 cut(s) 62, 741, 1542, 1910, 2367
BslI CCNNNNNNNGG 8 cut(s) 392, 485, 595, 690, 764, 973, 1900, 2259
BsmAI GTCTC 4 cut(s) 286, 1069, 1143, 1805
BsmBI CGTCTC 2 cut(s) 286, 1805
BsmFI GGGAC 5 cut(s) 62, 741, 1542, 1910, 2367
BsmI GAATGC 1 cut(s) 1105
Bsp119I TTCGAA 1 cut(s) 1670
Bsp120I GGGCCC 1 cut(s) 1187
Bsp1286I GDGCHC 5 cut(s) 330, 385, 502, 1191, 2310
Bsp1407I TGTACA 2 cut(s) 115, 2153
Bsp143I GATC 4 cut(s) 1180, 1370, 1694, 2203
Bsp19I CCATGG 1 cut(s) 1057
BspACI CCGC 7 cut(s) 80, 378, 390, 1626, 1709, 1987, 2041
BspCNI CTCAG 1 cut(s) 1153
BspDI ATCGAT 1 cut(s) 573
BspFNI CGCG 3 cut(s) 378, 390, 2197
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 5 cut(s) 682, 709, 1189, 1396, 2381
BspMAI CTGCAG 1 cut(s) 1495
BspPI GGATC 2 cut(s) 1378, 2211
BspQI GCTCTTC 1 cut(s) 279
BspT104I TTCGAA 1 cut(s) 1670
BspT107I GGYRCC 2 cut(s) 680, 1394
BsrDI GCAATG 2 cut(s) 1179, 1824
BsrFI RCCGGY 1 cut(s) 2088
BsrGI TGTACA 2 cut(s) 115, 2153
BsrI ACTGG 9 cut(s) 51, 424, 695, 1038, 1316, 1323, 1554, 1627, 2315
BssAI RCCGGY 1 cut(s) 2088
BssECI CCNNGG 6 cut(s) 370, 397, 418, 967, 1057, 1968
BssMI GATC 4 cut(s) 1180, 1370, 1694, 2203
BssNI GRCGYC 4 cut(s) 295, 1226, 1296, 1395
BssT1I CCWWGG 2 cut(s) 1057, 1968
Bst2UI CCWGG 5 cut(s) 123, 449, 2161, 2384, 2409
Bst4CI ACNGT 5 cut(s) 76, 313, 968, 1512, 1613
Bst6I CTCTTC 2 cut(s) 279, 343
BstACI GRCGYC 4 cut(s) 295, 1226, 1296, 1395
BstAPI GCANNNNNTGC 1 cut(s) 2314
BstAUI TGTACA 2 cut(s) 115, 2153
BstBI TTCGAA 1 cut(s) 1670
BstC8I GCNNGC 6 cut(s) 256, 1597, 1858, 1862, 2039, 2393
BstDEI CTNAG 2 cut(s) 1140, 1363
BstDSI CCRYGG 5 cut(s) 370, 397, 418, 967, 1057
BstEII GGTNACC 1 cut(s) 171
BstENI CCTNNNNNAGG 1 cut(s) 390
BstF5I GGATG 6 cut(s) 799, 1099, 1890, 2319, 2335, 2368
BstFNI CGCG 3 cut(s) 378, 390, 2197
BstH2I RGCGCY 1 cut(s) 1398
BstHHI GCGC 3 cut(s) 1397, 1483, 1866
BstKTI GATC 4 cut(s) 1183, 1373, 1697, 2206
BstMAI GTCTC 4 cut(s) 286, 1069, 1143, 1805
BstMBI GATC 4 cut(s) 1180, 1370, 1694, 2203
BstMCI CGRYCG 1 cut(s) 2089
BstNI CCWGG 5 cut(s) 123, 449, 2161, 2384, 2409
BstNSI RCATGY 4 cut(s) 258, 1532, 1864, 2395
BstPI GGTNACC 1 cut(s) 171
BstSCI CCNGG 7 cut(s) 121, 447, 1220, 1893, 2159, 2382, 2407
BstSFI CTRYAG 2 cut(s) 1274, 1491
BstSLI GKGCMC 2 cut(s) 1191, 2310
BstUI CGCG 3 cut(s) 378, 390, 2197
BstX2I RGATCY 2 cut(s) 1180, 1370
BstXI CCANNNNNNTGG 3 cut(s) 524, 2346, 2424
BstYI RGATCY 2 cut(s) 1180, 1370
Bsu15I ATCGAT 1 cut(s) 573
BsuI GTATCC 2 cut(s) 141, 542
BsuTUI ATCGAT 1 cut(s) 573
BtgI CCRYGG 5 cut(s) 370, 397, 418, 967, 1057
BtrI CACGTC 3 cut(s) 59, 1421, 2097
BtsCI GGATG 6 cut(s) 799, 1099, 1890, 2319, 2335, 2368
BtsI GCAGTG 4 cut(s) 330, 877, 1390, 1630
Cac8I GCNNGC 6 cut(s) 256, 1597, 1858, 1862, 2039, 2393
CciI TCATGA 1 cut(s) 481
CfoI GCGC 3 cut(s) 1397, 1483, 1866
Cfr10I RCCGGY 1 cut(s) 2088
Cfr13I GGNCC 6 cut(s) 976, 1187, 1188, 2091, 2262, 2380
ClaI ATCGAT 1 cut(s) 573
CseI GACGC 3 cut(s) 1234, 1304, 1592
CsiI ACCWGGT 1 cut(s) 2407
Csp6I GTAC 4 cut(s) 116, 1161, 2154, 2414
CspAI ACCGGT 1 cut(s) 2088
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviQI GTAC 4 cut(s) 116, 1161, 2154, 2414
DdeI CTNAG 2 cut(s) 1140, 1363
DinI GGCGCC 1 cut(s) 1396
DpnI GATC 4 cut(s) 1182, 1372, 1696, 2205
DpnII GATC 4 cut(s) 1180, 1370, 1694, 2203
EaeI YGGCCR 4 cut(s) 415, 421, 526, 559
Eam1104I CTCTTC 2 cut(s) 279, 343
EarI CTCTTC 2 cut(s) 279, 343
EciI GGCGGA 1 cut(s) 1724
Eco130I CCWWGG 2 cut(s) 1057, 1968
Eco24I GRGCYC 2 cut(s) 330, 1191
Eco47I GGWCC 2 cut(s) 976, 2091
Eco91I GGTNACC 1 cut(s) 171
EcoNI CCTNNNNNAGG 1 cut(s) 390
EcoO109I RGGNCCY 2 cut(s) 976, 1188
EcoO65I GGTNACC 1 cut(s) 171
EcoRII CCWGG 5 cut(s) 121, 447, 2159, 2382, 2407
EcoT14I CCWWGG 2 cut(s) 1057, 1968
EcoT22I ATGCAT 1 cut(s) 2397
EcoT38I GRGCYC 2 cut(s) 330, 1191
EgeI GGCGCC 1 cut(s) 1396
EheI GGCGCC 1 cut(s) 1396
ErhI CCWWGG 2 cut(s) 1057, 1968
Esp3I CGTCTC 2 cut(s) 286, 1805
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FaqI GGGAC 5 cut(s) 62, 741, 1542, 1910, 2367
FauI CCCGC 2 cut(s) 87, 2034
FokI GGATG 6 cut(s) 806, 1106, 1877, 2322, 2326, 2375
FriOI GRGCYC 2 cut(s) 330, 1191
FspBI CTAG 8 cut(s) 20, 168, 200, 1110, 1698, 2058, 2148, 2291
FspI TGCGCA 1 cut(s) 1482
GlaI GCGC 3 cut(s) 1396, 1482, 1865
GsuI CTGGAG 1 cut(s) 726
HaeII RGCGCY 1 cut(s) 1398
HapII CCGG 5 cut(s) 659, 1009, 1222, 1894, 2089
HgaI GACGC 3 cut(s) 1234, 1304, 1592
HhaI GCGC 3 cut(s) 1397, 1483, 1866
Hin1I GRCGYC 4 cut(s) 295, 1226, 1296, 1395
Hin6I GCGC 3 cut(s) 1395, 1481, 1864
HinP1I GCGC 3 cut(s) 1395, 1481, 1864
HincII GTYRAC 5 cut(s) 1054, 1444, 1721, 2024, 2431
HindII GTYRAC 5 cut(s) 1054, 1444, 1721, 2024, 2431
HindIII AAGCTT 3 cut(s) 302, 633, 2009
HinfI GANTC 6 cut(s) 103, 701, 736, 1062, 2141, 2449
HpaII CCGG 5 cut(s) 659, 1009, 1222, 1894, 2089
HphI GGTGA 2 cut(s) 715, 1364
Hpy188I TCNGA 4 cut(s) 27, 1093, 2065, 2275
Hpy188III TCNNGA 7 cut(s) 360, 482, 705, 974, 1424, 1692, 2207
Hpy99I CGWCG 2 cut(s) 555, 1617
HpyAV CCTTC 7 cut(s) 516, 872, 884, 1239, 1409, 1585, 2123
HpyCH4III ACNGT 5 cut(s) 76, 313, 968, 1512, 1613
HpyCH4IV ACGT 7 cut(s) 58, 295, 610, 1420, 1681, 2096, 2454
HpyF3I CTNAG 2 cut(s) 1140, 1363
HpySE526I ACGT 7 cut(s) 58, 295, 610, 1420, 1681, 2096, 2454
Hsp92I GRCGYC 4 cut(s) 295, 1226, 1296, 1395
HspAI GCGC 3 cut(s) 1395, 1481, 1864
KasI GGCGCC 1 cut(s) 1394
Kzo9I GATC 4 cut(s) 1180, 1370, 1694, 2203
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 6 cut(s) 388, 466, 505, 625, 707, 1267
LweI GCATC 7 cut(s) 218, 671, 984, 1084, 1512, 2304, 2320
MabI ACCWGGT 1 cut(s) 2407
MaeI CTAG 8 cut(s) 20, 168, 200, 1110, 1698, 2058, 2148, 2291
MaeII ACGT 7 cut(s) 58, 295, 610, 1420, 1681, 2096, 2454
MaeIII GTNAC 4 cut(s) 171, 1628, 1689, 1808
MalI GATC 4 cut(s) 1182, 1372, 1696, 2205
MboI GATC 4 cut(s) 1180, 1370, 1694, 2203
MboII GAAGA 8 cut(s) 266, 269, 360, 617, 1003, 1035, 1365, 1772
MflI RGATCY 2 cut(s) 1180, 1370
MhlI GDGCHC 5 cut(s) 330, 385, 502, 1191, 2310
MlsI TGGCCA 2 cut(s) 423, 561
MluCI AATT 6 cut(s) 917, 958, 1651, 1656, 1950, 2343
MluI ACGCGT 1 cut(s) 2195
MluNI TGGCCA 2 cut(s) 423, 561
Mly113I GGCGCC 1 cut(s) 1395
MlyI GAGTC 2 cut(s) 695, 1071
MmeI TCCRAC 5 cut(s) 536, 1071, 1496, 2273, 2442
Mox20I TGGCCA 2 cut(s) 423, 561
Mph1103I ATGCAT 1 cut(s) 2397
MroXI GAANNNNTTC 4 cut(s) 102, 273, 612, 2140
MscI TGGCCA 2 cut(s) 423, 561
MseI TTAA 3 cut(s) 780, 1655, 1964
MslI CAYNNNNRTG 2 cut(s) 369, 408
Msp20I TGGCCA 2 cut(s) 423, 561
MspA1I CMGCKG 2 cut(s) 82, 113
MspI CCGG 5 cut(s) 659, 1009, 1222, 1894, 2089
MspR9I CCNGG 7 cut(s) 123, 449, 1222, 1895, 2161, 2384, 2409
Mva1269I GAATGC 1 cut(s) 1105
MvaI CCWGG 5 cut(s) 123, 449, 2161, 2384, 2409
MvnI CGCG 3 cut(s) 378, 390, 2197
NarI GGCGCC 1 cut(s) 1395
NciI CCSGG 2 cut(s) 1222, 1895
NcoI CCATGG 1 cut(s) 1057
NdeII GATC 4 cut(s) 1180, 1370, 1694, 2203
NlaIV GGNNCC 5 cut(s) 682, 709, 1189, 1396, 2381
NmuCI GTSAC 3 cut(s) 1628, 1689, 1808
NsbI TGCGCA 1 cut(s) 1482
NsiI ATGCAT 1 cut(s) 2397
NspI RCATGY 4 cut(s) 258, 1532, 1864, 2395
NspV TTCGAA 1 cut(s) 1670
PaeI GCATGC 3 cut(s) 258, 1864, 2395
PagI TCATGA 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 279
PcsI WCGNNNNNNNCGW 1 cut(s) 394
PctI GAATGC 1 cut(s) 1105
PdmI GAANNNNTTC 4 cut(s) 102, 273, 612, 2140
PfeI GAWTC 4 cut(s) 103, 736, 2141, 2449
PflFI GACNNNGTC 1 cut(s) 1901
PflMI CCANNNNNTGG 1 cut(s) 2259
PfoI TCCNGGA 2 cut(s) 1220, 1893
PinAI ACCGGT 1 cut(s) 2088
PleI GAGTC 2 cut(s) 695, 1070
PluTI GGCGCC 1 cut(s) 1398
PpsI GAGTC 2 cut(s) 695, 1070
PpuMI RGGWCCY 1 cut(s) 976
Psp5II RGGWCCY 1 cut(s) 976
Psp6I CCWGG 5 cut(s) 121, 447, 2159, 2382, 2407
PspEI GGTNACC 1 cut(s) 171
PspGI CCWGG 5 cut(s) 121, 447, 2159, 2382, 2407
PspN4I GGNNCC 5 cut(s) 682, 709, 1189, 1396, 2381
PspOMI GGGCCC 1 cut(s) 1187
PspPI GGNCC 6 cut(s) 976, 1187, 1188, 2091, 2262, 2380
PspPPI RGGWCCY 1 cut(s) 976
PstI CTGCAG 1 cut(s) 1495
PsuI RGATCY 2 cut(s) 1180, 1370
PsyI GACNNNGTC 1 cut(s) 1901
PvuII CAGCTG 1 cut(s) 113
RsaI GTAC 4 cut(s) 117, 1162, 2155, 2415
RsaNI GTAC 4 cut(s) 116, 1161, 2154, 2414
RseI CAYNNNNRTG 2 cut(s) 369, 408
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 3 cut(s) 780, 1655, 1964
Sau3AI GATC 4 cut(s) 1180, 1370, 1694, 2203
Sau96I GGNCC 6 cut(s) 976, 1187, 1188, 2091, 2262, 2380
SchI GAGTC 2 cut(s) 695, 1071
ScrFI CCNGG 7 cut(s) 123, 449, 1222, 1895, 2161, 2384, 2409
SduI GDGCHC 5 cut(s) 330, 385, 502, 1191, 2310
SexAI ACCWGGT 1 cut(s) 2407
SfaNI GCATC 7 cut(s) 218, 671, 984, 1084, 1512, 2304, 2320
SfcI CTRYAG 2 cut(s) 1274, 1491
SfoI GGCGCC 1 cut(s) 1396
SfuI TTCGAA 1 cut(s) 1670
SinI GGWCC 2 cut(s) 976, 2091
SmiMI CAYNNNNRTG 2 cut(s) 369, 408
SmlI CTYRAG 2 cut(s) 441, 1336
SmoI CTYRAG 2 cut(s) 441, 1336
SphI GCATGC 3 cut(s) 258, 1864, 2395
Sse9I AATT 6 cut(s) 917, 958, 1651, 1656, 1950, 2343
SsiI CCGC 7 cut(s) 80, 378, 390, 1626, 1709, 1987, 2041
SspDI GGCGCC 1 cut(s) 1394
SspMI CTAG 8 cut(s) 20, 168, 200, 1110, 1698, 2058, 2148, 2291
StyD4I CCNGG 7 cut(s) 121, 447, 1220, 1893, 2159, 2382, 2407
StyI CCWWGG 2 cut(s) 1057, 1968
TaaI ACNGT 5 cut(s) 76, 313, 968, 1512, 1613
TaiI ACGT 7 cut(s) 61, 298, 613, 1423, 1684, 2099, 2457
TaqI TCGA 3 cut(s) 573, 1670, 2230
TasI AATT 6 cut(s) 917, 958, 1651, 1656, 1950, 2343
TatI WGTACW 2 cut(s) 115, 2153
TfiI GAWTC 4 cut(s) 103, 736, 2141, 2449
Tru1I TTAA 3 cut(s) 780, 1655, 1964
Tru9I TTAA 3 cut(s) 780, 1655, 1964
TseFI GTSAC 3 cut(s) 1628, 1689, 1808
Tsp45I GTSAC 3 cut(s) 1628, 1689, 1808
TspDTI ATGAA 5 cut(s) 296, 618, 1190, 1450, 1650
TspGWI ACGGA 1 cut(s) 1757
Tth111I GACNNNGTC 1 cut(s) 1901
Van91I CCANNNNNTGG 1 cut(s) 2259
VpaK11BI GGWCC 2 cut(s) 976, 2091
XagI CCTNNNNNAGG 1 cut(s) 390
XceI RCATGY 4 cut(s) 258, 1532, 1864, 2395
XcmI CCANNNNNNNNNTGG 4 cut(s) 1045, 1261, 1800, 2256
XmnI GAANNNNTTC 4 cut(s) 102, 273, 612, 2140
XspI CTAG 8 cut(s) 20, 168, 200, 1110, 1698, 2058, 2148, 2291
ZraI GACGTC 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 2397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.