MD14G1109000.v1.1
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000889
Physical Location & Seq
Forward (+)
17132589 .. 17137304
4716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1109000.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGAAATCCAAATTCACTACACCCAGTTTTGTTGAAGTTTCTCTGTGGCCTTTTGGCTCTGATATTTCTTCGGGGAAGATGCTTTTTAGTGGACCAGTTCGTGATGGATTCTACCCTTTTCATACTTTGTCTCCTGCTCCTGGTTCTCCACACTTGTTTGCTGCTACAACAATGGCTTCTCAGGATCTTTGGCACAAGAAACTTGGCCATCCGTCTACCAAAGTTTTAAATAAATTGGCCTCTACTTCGTGTATTTCTATGTCTCGTACTATGAATAACACTTTCTGTTCCGATTATGCTGTAAGAAAAAGCTCTAAGCTTCCTTTTGTTTCTAGTTCTTGTACTACAAGCAAGCCTTTAGAGCTTTTTCACATGGATGTCTGGGTACCAGCTCCTATTTCCTCTGTACATGGTTTTCGATACTATGTGATAATTGTTGATAACTATACAAAGTATTCTTGGCTTTATCCTCTGAAGTTCAAGTATGATGTTCTTTCTACATTTGTTCACTTCAAGTCTCTTATTGAAAATAAGTTGGGTGTCAAAGTTATTTCTATTCGCTCAGATTCTGGTGGTGAATTCTTAAGCTCCAAGTTCTCACAGTTCTTACTTGACCATGGTATTACACACCAACTCAGCTGTCCTCACACTCCTAAGCAAAATGGGTGTGCTGAGCGCAAGCACCGACATCTTGTTGAAACTGCCTGTACTCTATTGACAGCATCAAAATATTCCCACTGGAGACAGGCAATGCAAGAAGAGTTCAATGCTCTTCTCAACAATGGTATTTGGTCCTTGGTTCCCTATTCTCCTTCGCAAATTCTAGTAGGTTGCAAGTGGGTATTTCGAATCAAACACAAGCCTGATGGATCTATAGATCGGTATAAGGCCCGATTGGTTGCAAAGGGGTTTCATCAACAGGAAGGGCTAGACTACATAGAGACTGTCAGCCCTGTTGCCAAACCTGCCACCATTCGCCTTTTACTCACTTTGGCTGCTCAATATGATTGGTTTCTCAACCAACTTGATGTCAGCAATGCTTTTCTGCATGGTACTCTTACAGAAACTGTGTTTATACAACAACTTCCAAGGTTTCAGGATTTTACACAACCTGATTCTGTTTGCAGACTTCATAAATCATTGTATGGTATCAAACAAGCTCCTCGAGCTTGGTATGATAAACTACATGGTGCCTTACTCTCCTTGGGATTCGTTGGCTCAAAATCGAATAATTCATTATCTGCCAACTTATTGCTGATATTGGTCTTCCACTACCCTGTGTACCAAAGTTGTGGTGTGACAATATATCCCATTTTCCATGCTCGCACTAAGCATGTTGAATTGGACTATCACTACATCCGGGAAAAGGTTTTGGCTCATGAAATTTCTCTGCATTTTGTTTGCTCCCAGGATCAGATAGTTGATATTTGTACCAAGGCTCTGTCCAAATCACGATTTATGTTTCTCCGGTCCAAACTTGCTCTTCGGCAACCTCAGTTCAGTTTGAAGGGGGATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

507

Amino Acids

57.46

Weight (kDa)

9.43

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 50 - 103 4.7e-08 GAG-pre-integrase domain
rve PF00665 118 - 218 2.4e-13 Integrase core domain
RVT_2 PF07727 262 - 410 2.4e-41 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 973
Acc65I GGTACC 1 cut(s) 385
AccB1I GGYRCC 2 cut(s) 385, 1190
AccI GTMKAC 1 cut(s) 215
AclWI GGATC 3 cut(s) 192, 877, 1419
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 4 cut(s) 11, 578, 819, 1383
AcuI CTGAAG 1 cut(s) 494
AfaI GTAC 8 cut(s) 268, 343, 387, 408, 709, 1054, 1283, 1432
AfiI CCNNNNNNNGG 2 cut(s) 140, 1366
AflII CTTAAG 1 cut(s) 583
AgsI TTSAA 8 cut(s) 35, 481, 514, 527, 698, 766, 1340, 1507
AjnI CCWGG 2 cut(s) 139, 1407
AluBI AGCT 8 cut(s) 312, 319, 364, 392, 588, 639, 1160, 1169
AluI AGCT 8 cut(s) 312, 319, 364, 392, 588, 639, 1160, 1169
Alw26I GTCTC 5 cut(s) 135, 267, 522, 736, 935
AlwI GGATC 3 cut(s) 192, 877, 1419
AlwNI CAGNNNCTG 2 cut(s) 569, 1067
Ama87I CYCGRG 1 cut(s) 1164
AoxI GGCC 4 cut(s) 47, 205, 237, 888
ApeKI GCWGC 2 cut(s) 161, 995
ApoI RAATTY 4 cut(s) 11, 578, 819, 1383
Asp718I GGTACC 1 cut(s) 385
AspLEI GCGC 1 cut(s) 678
AspS9I GGNCC 4 cut(s) 92, 792, 889, 1470
AsuC2I CCSGG 1 cut(s) 1361
AsuHPI GGTGA 1 cut(s) 587
AsuII TTCGAA 1 cut(s) 847
AvaI CYCGRG 1 cut(s) 1164
AvaII GGWCC 3 cut(s) 92, 792, 1470
BalI TGGCCA 1 cut(s) 207
BanI GGYRCC 2 cut(s) 385, 1190
BbsI GAAGAC 1 cut(s) 1258
BbvI GCAGC 2 cut(s) 148, 982
BccI CCATC 3 cut(s) 98, 216, 860
BciT130I CCWGG 2 cut(s) 141, 1409
BcnI CCSGG 1 cut(s) 1361
BcoDI GTCTC 5 cut(s) 135, 267, 522, 736, 935
BfaI CTAG 3 cut(s) 333, 824, 929
BfmI CTRYAG 1 cut(s) 873
BfrI CTTAAG 1 cut(s) 583
BfuAI ACCTGC 1 cut(s) 973
BisI GCNGC 2 cut(s) 162, 996
BlpI GCTNAGC 1 cut(s) 672
BlsI GCNGC 2 cut(s) 163, 997
Bme1390I CCNGG 3 cut(s) 141, 1361, 1409
Bme18I GGWCC 3 cut(s) 92, 792, 1470
BmeT110I CYCGRG 1 cut(s) 1164
BmgT120I GGNCC 4 cut(s) 92, 792, 889, 1470
BmiI GGNNCC 3 cut(s) 387, 801, 1192
BmrFI CCNGG 3 cut(s) 141, 1361, 1409
BmrI ACTGGG 1 cut(s) 18
BmsI GCATC 2 cut(s) 69, 731
BmuI ACTGGG 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 1258
BpmI CTGGAG 1 cut(s) 760
Bpu10I CCTNAGC 1 cut(s) 654
Bpu1102I GCTNAGC 1 cut(s) 672
Bpu14I TTCGAA 1 cut(s) 847
BpuMI CCSGG 1 cut(s) 1361
BsaJI CCNNGG 6 cut(s) 616, 795, 1088, 1203, 1407, 1434
BsaWI WCCGGW 1 cut(s) 1467
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 1366
Bse1I ACTGG 3 cut(s) 24, 95, 743
Bse3DI GCAATG 2 cut(s) 756, 1042
BseBI CCWGG 2 cut(s) 141, 1409
BseDI CCNNGG 6 cut(s) 616, 795, 1088, 1203, 1407, 1434
BseGI GGATG 3 cut(s) 208, 382, 1356
BseLI CCNNNNNNNGG 2 cut(s) 140, 1366
BseMI GCAATG 2 cut(s) 756, 1042
BseMII CTCAG 5 cut(s) 194, 576, 649, 663, 1508
BseNI ACTGG 3 cut(s) 24, 95, 743
BseRI GAGGAG 1 cut(s) 1152
BseXI GCAGC 2 cut(s) 148, 982
BshFI GGCC 4 cut(s) 49, 207, 239, 890
BshNI GGYRCC 2 cut(s) 385, 1190
BsiHKCI CYCGRG 1 cut(s) 1164
BsiSI CCGG 2 cut(s) 1360, 1468
BslI CCNNNNNNNGG 2 cut(s) 140, 1366
BsmAI GTCTC 5 cut(s) 135, 267, 522, 736, 935
BsnI GGCC 4 cut(s) 49, 207, 239, 890
BsoBI CYCGRG 1 cut(s) 1164
Bsp119I TTCGAA 1 cut(s) 847
Bsp1407I TGTACA 1 cut(s) 406
Bsp143I GATC 4 cut(s) 184, 869, 877, 1411
Bsp1720I GCTNAGC 1 cut(s) 672
Bsp19I CCATGG 1 cut(s) 616
BspANI GGCC 4 cut(s) 49, 207, 239, 890
BspCNI CTCAG 5 cut(s) 193, 575, 648, 664, 1507
BspHI TCATGA 1 cut(s) 1378
BspLI GGNNCC 3 cut(s) 387, 801, 1192
BspMI ACCTGC 1 cut(s) 973
BspPI GGATC 3 cut(s) 192, 877, 1419
BspQI GCTCTTC 2 cut(s) 777, 1488
BspT104I TTCGAA 1 cut(s) 847
BspT107I GGYRCC 2 cut(s) 385, 1190
BspTI CTTAAG 1 cut(s) 583
BsrDI GCAATG 2 cut(s) 756, 1042
BsrGI TGTACA 1 cut(s) 406
BsrI ACTGG 3 cut(s) 24, 95, 743
BssECI CCNNGG 6 cut(s) 616, 795, 1088, 1203, 1407, 1434
BssMI GATC 4 cut(s) 184, 869, 877, 1411
BssT1I CCWWGG 5 cut(s) 616, 795, 1088, 1203, 1434
Bst2UI CCWGG 2 cut(s) 141, 1409
Bst4CI ACNGT 3 cut(s) 603, 946, 1069
Bst6I CTCTTC 3 cut(s) 753, 777, 1488
BstAFI CTTAAG 1 cut(s) 583
BstAUI TGTACA 1 cut(s) 406
BstBI TTCGAA 1 cut(s) 847
BstC8I GCNNGC 3 cut(s) 353, 680, 1324
BstDEI CTNAG 8 cut(s) 180, 315, 562, 635, 654, 672, 1329, 1494
BstDSI CCRYGG 1 cut(s) 616
BstF5I GGATG 3 cut(s) 208, 382, 1356
BstHHI GCGC 1 cut(s) 678
BstKTI GATC 4 cut(s) 187, 872, 880, 1414
BstMAI GTCTC 5 cut(s) 135, 267, 522, 736, 935
BstMBI GATC 4 cut(s) 184, 869, 877, 1411
BstMWI GCNNNNNNNGC 3 cut(s) 361, 965, 1166
BstNI CCWGG 2 cut(s) 141, 1409
BstNSI RCATGY 1 cut(s) 1337
BstSCI CCNGG 3 cut(s) 139, 1359, 1407
BstSFI CTRYAG 1 cut(s) 873
BstV1I GCAGC 2 cut(s) 148, 982
BstV2I GAAGAC 1 cut(s) 1258
BstX2I RGATCY 2 cut(s) 184, 869
BstXI CCANNNNNNTGG 1 cut(s) 1292
BstYI RGATCY 2 cut(s) 184, 869
BsuRI GGCC 4 cut(s) 49, 207, 239, 890
BtgI CCRYGG 1 cut(s) 616
BtsCI GGATG 3 cut(s) 208, 382, 1356
BtsIMutI CAGTG 1 cut(s) 736
BveI ACCTGC 1 cut(s) 973
Cac8I GCNNGC 3 cut(s) 353, 680, 1324
CaiI CAGNNNCTG 2 cut(s) 569, 1067
CciI TCATGA 1 cut(s) 1378
CfoI GCGC 1 cut(s) 678
Cfr13I GGNCC 4 cut(s) 92, 792, 889, 1470
Csp6I GTAC 8 cut(s) 267, 342, 386, 407, 708, 1053, 1282, 1431
CviAII CATG 8 cut(s) 373, 410, 617, 1049, 1187, 1319, 1334, 1379
CviQI GTAC 8 cut(s) 267, 342, 386, 407, 708, 1053, 1282, 1431
DdeI CTNAG 8 cut(s) 180, 315, 562, 635, 654, 672, 1329, 1494
DpnI GATC 4 cut(s) 186, 871, 879, 1413
DpnII GATC 4 cut(s) 184, 869, 877, 1411
DraI TTTAAA 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 3 cut(s) 753, 777, 1488
EarI CTCTTC 3 cut(s) 753, 777, 1488
Eco130I CCWWGG 5 cut(s) 616, 795, 1088, 1203, 1434
Eco47I GGWCC 3 cut(s) 92, 792, 1470
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 1164
EcoRI GAATTC 1 cut(s) 578
EcoRII CCWGG 2 cut(s) 139, 1407
EcoT14I CCWWGG 5 cut(s) 616, 795, 1088, 1203, 1434
ErhI CCWWGG 5 cut(s) 616, 795, 1088, 1203, 1434
FaeI CATG 8 cut(s) 376, 413, 620, 1052, 1190, 1322, 1337, 1382
FalI AAGNNNNNCTT 2 cut(s) 340, 372
FatI CATG 8 cut(s) 372, 409, 616, 1048, 1186, 1318, 1333, 1378
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 2 cut(s) 162, 996
FokI GGATG 3 cut(s) 195, 389, 1343
Fsp4HI GCNGC 2 cut(s) 162, 996
FspBI CTAG 3 cut(s) 333, 824, 929
GlaI GCGC 1 cut(s) 677
GluI GCNGC 2 cut(s) 162, 996
GsuI CTGGAG 1 cut(s) 760
HaeIII GGCC 4 cut(s) 49, 207, 239, 890
HapII CCGG 2 cut(s) 1360, 1468
HhaI GCGC 1 cut(s) 678
Hin1II CATG 8 cut(s) 376, 413, 620, 1052, 1190, 1322, 1337, 1382
Hin6I GCGC 1 cut(s) 676
HinP1I GCGC 1 cut(s) 676
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 5 cut(s) 108, 566, 849, 1115, 1209
HpaII CCGG 2 cut(s) 1360, 1468
HphI GGTGA 1 cut(s) 587
Hpy166II GTNNAC 4 cut(s) 92, 216, 508, 1282
Hpy188I TCNGA 5 cut(s) 61, 292, 474, 565, 1416
Hpy188III TCNNGA 5 cut(s) 101, 182, 1097, 1379, 1452
Hpy8I GTNNAC 4 cut(s) 92, 216, 508, 1282
HpyAV CCTTC 3 cut(s) 822, 917, 1501
HpyCH4III ACNGT 3 cut(s) 603, 946, 1069
HpyCH4V TGCA 6 cut(s) 754, 834, 902, 1048, 1125, 1393
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 965, 1166
HpyF3I CTNAG 8 cut(s) 180, 315, 562, 635, 654, 672, 1329, 1494
Hsp92II CATG 8 cut(s) 376, 413, 620, 1052, 1190, 1322, 1337, 1382
HspAI GCGC 1 cut(s) 676
KpnI GGTACC 1 cut(s) 389
Kzo9I GATC 4 cut(s) 184, 869, 877, 1411
LguI GCTCTTC 2 cut(s) 777, 1488
LmnI GCTCC 5 cut(s) 142, 397, 593, 1165, 1409
Lsp1109I GCAGC 2 cut(s) 148, 982
LweI GCATC 2 cut(s) 69, 731
MaeI CTAG 3 cut(s) 333, 824, 929
MaeIII GTNAC 1 cut(s) 1297
MalI GATC 4 cut(s) 186, 871, 879, 1413
MboI GATC 4 cut(s) 184, 869, 877, 1411
MboII GAAGA 6 cut(s) 60, 88, 764, 770, 1258, 1475
MflI RGATCY 2 cut(s) 184, 869
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 8 cut(s) 11, 233, 432, 578, 819, 1231, 1340, 1383
MluNI TGGCCA 1 cut(s) 207
MnlI CCTC 6 cut(s) 250, 412, 480, 654, 1173, 1503
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 2 cut(s) 227, 584
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 639
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 2 cut(s) 1360, 1468
MspR9I CCNGG 3 cut(s) 141, 1361, 1409
MvaI CCWGG 2 cut(s) 141, 1409
MwoI GCNNNNNNNGC 3 cut(s) 361, 965, 1166
NciI CCSGG 1 cut(s) 1361
NcoI CCATGG 1 cut(s) 616
NdeII GATC 4 cut(s) 184, 869, 877, 1411
NlaIII CATG 8 cut(s) 376, 413, 620, 1052, 1190, 1322, 1337, 1382
NlaIV GGNNCC 3 cut(s) 387, 801, 1192
NmuCI GTSAC 1 cut(s) 1297
NspI RCATGY 1 cut(s) 1337
NspV TTCGAA 1 cut(s) 847
PaeR7I CTCGAG 1 cut(s) 1164
PagI TCATGA 1 cut(s) 1378
PciSI GCTCTTC 2 cut(s) 777, 1488
PfeI GAWTC 5 cut(s) 108, 566, 849, 1115, 1209
PfoI TCCNGGA 1 cut(s) 1359
PkrI GCNGC 2 cut(s) 163, 997
Psp6I CCWGG 2 cut(s) 139, 1407
PspGI CCWGG 2 cut(s) 139, 1407
PspN4I GGNNCC 3 cut(s) 387, 801, 1192
PspPI GGNCC 4 cut(s) 92, 792, 889, 1470
PspXI VCTCGAGB 1 cut(s) 1164
PstNI CAGNNNCTG 2 cut(s) 569, 1067
PsuI RGATCY 2 cut(s) 184, 869
PvuII CAGCTG 1 cut(s) 639
RsaI GTAC 8 cut(s) 268, 343, 387, 408, 709, 1054, 1283, 1432
RsaNI GTAC 8 cut(s) 267, 342, 386, 407, 708, 1053, 1282, 1431
RseI CAYNNNNRTG 1 cut(s) 375
SapI GCTCTTC 2 cut(s) 777, 1488
SaqAI TTAA 2 cut(s) 227, 584
SatI GCNGC 2 cut(s) 162, 996
Sau3AI GATC 4 cut(s) 184, 869, 877, 1411
Sau96I GGNCC 4 cut(s) 92, 792, 889, 1470
ScrFI CCNGG 3 cut(s) 141, 1361, 1409
SfaNI GCATC 2 cut(s) 69, 731
SfcI CTRYAG 1 cut(s) 873
Sfr274I CTCGAG 1 cut(s) 1164
SfuI TTCGAA 1 cut(s) 847
SinI GGWCC 3 cut(s) 92, 792, 1470
SlaI CTCGAG 1 cut(s) 1164
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 2 cut(s) 583, 1164
SmoI CTYRAG 2 cut(s) 583, 1164
Sse9I AATT 8 cut(s) 11, 233, 432, 578, 819, 1231, 1340, 1383
SspI AATATT 1 cut(s) 731
SspMI CTAG 3 cut(s) 333, 824, 929
StyD4I CCNGG 3 cut(s) 139, 1359, 1407
StyI CCWWGG 5 cut(s) 616, 795, 1088, 1203, 1434
TaaI ACNGT 3 cut(s) 603, 946, 1069
TaqI TCGA 4 cut(s) 418, 847, 1165, 1226
TasI AATT 8 cut(s) 11, 233, 432, 578, 819, 1231, 1340, 1383
TatI WGTACW 3 cut(s) 341, 406, 707
TfiI GAWTC 5 cut(s) 108, 566, 849, 1115, 1209
Tru1I TTAA 2 cut(s) 227, 584
Tru9I TTAA 2 cut(s) 227, 584
TscAI CASTG 1 cut(s) 743
TseFI GTSAC 1 cut(s) 1297
TseI GCWGC 2 cut(s) 161, 995
Tsp45I GTSAC 1 cut(s) 1297
TspDTI ATGAA 7 cut(s) 17, 110, 287, 902, 1121, 1224, 1395
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 743
Vha464I CTTAAG 1 cut(s) 583
VpaK11BI GGWCC 3 cut(s) 92, 792, 1470
XapI RAATTY 4 cut(s) 11, 578, 819, 1383
XceI RCATGY 1 cut(s) 1337
XhoI CTCGAG 1 cut(s) 1164
XmiI GTMKAC 1 cut(s) 215
XspI CTAG 3 cut(s) 333, 824, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.