MD03G1029600.v1.1

pre-rRNA-processing protein TSR2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000787
Physical Location & Seq
Forward (+)
2413253 .. 2415400
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1029600.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGAGCCTGAGAGAAAGCTATCGGCGGAGGCTGGCTCCGTATTCCAGGAAGGCATAGGATTGGTTCTGTCTCGGTGGTCAGCGCTCCAATTGGCCGTCGAGAACGAGTGGGGCGGTCGTGACTCGCGCCGCAAAGCGGAGCAACTCGTCGCCGATATCTTCTCCTGGTTCAATCACTCCACAGAGCCTCTTTACATAGATGATTTGGAAGATATGCTGAATGAAGCTATGCTTTCTCTCAACACTATGACAGAGGATGGCAGCATCGAGGAGGTAGCTGAAAAGTTAATGATTATGCATGAAGAGTGTTTAAATTGTAATTTCAAGTCGATTGAAAGCCTAAGGGAAGCCAATCAGCGAAGAGTTGCTCTTCCGCATGTTAGAGAGGTTGCAAATGATGATGACGAGGATAGTGACGAAGACAATGATAATATAGCTCATAGCATGGGAAATGATGACTCCTCAAACATGATGGTAGACATACCGGAGGCTCATTTGAACTTAAATCCCGTAGACGTCTCCAGCAATGAGTCAAAGCCCAAGCCGTCGGCTGAAGCAGAAGATGGATGGGAGGTAGTTGGACCTAGAAAACATAGGGGTAAAAGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WGG

Protein Analysis

204

Amino Acids

22.88

Weight (kDa)

4.45

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WGG PF10273 15 - 94 5e-26 Pre-rRNA-processing protein TSR2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 519
AccI GTMKAC 2 cut(s) 477, 513
AccII CGCG 1 cut(s) 127
AciI CCGC 5 cut(s) 26, 114, 130, 137, 374
AcoI YGGCCR 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 573
AcyI GRCGYC 1 cut(s) 516
AfeI AGCGCT 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 136
AgsI TTSAA 4 cut(s) 172, 325, 335, 499
AjnI CCWGG 2 cut(s) 45, 164
AluBI AGCT 4 cut(s) 19, 227, 278, 437
AluI AGCT 4 cut(s) 19, 227, 278, 437
Alw26I GTCTC 2 cut(s) 75, 523
Aor51HI AGCGCT 1 cut(s) 84
AoxI GGCC 1 cut(s) 93
ApeKI GCWGC 1 cut(s) 261
AspLEI GCGC 2 cut(s) 85, 129
AspS9I GGNCC 1 cut(s) 581
AvaII GGWCC 1 cut(s) 581
AxyI CCTNAGG 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 273
BccI CCATC 4 cut(s) 251, 466, 557, 561
BceAI ACGGC 2 cut(s) 80, 529
BciT130I CCWGG 2 cut(s) 47, 166
BcoDI GTCTC 2 cut(s) 75, 523
BfaI CTAG 1 cut(s) 585
BfoI RGCGCY 1 cut(s) 86
BisI GCNGC 2 cut(s) 130, 262
BlsI GCNGC 2 cut(s) 131, 263
Bme1390I CCNGG 2 cut(s) 47, 166
Bme18I GGWCC 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 2 cut(s) 6, 37
BmrFI CCNGG 2 cut(s) 47, 166
BmsI GCATC 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 426
BplI GAGNNNNNCTC 2 cut(s) 20, 52
BpmI CTGGAG 1 cut(s) 505
BsaHI GRCGYC 1 cut(s) 516
BsaWI WCCGGW 1 cut(s) 484
Bsc4I CCNNNNNNNGG 1 cut(s) 136
Bse21I CCTNAGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 2 cut(s) 47, 166
BseGI GGATG 2 cut(s) 262, 572
BseLI CCNNNNNNNGG 1 cut(s) 136
BseMI GCAATG 1 cut(s) 532
BseRI GAGGAG 2 cut(s) 284, 451
BseXI GCAGC 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 127
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 1 cut(s) 95
BsiEI CGRYCG 1 cut(s) 118
BsiSI CCGG 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 136
BsmAI GTCTC 2 cut(s) 75, 523
BsmBI CGTCTC 1 cut(s) 523
BsnI GGCC 1 cut(s) 95
BspACI CCGC 5 cut(s) 26, 114, 130, 137, 374
BspANI GGCC 1 cut(s) 95
BspFNI CGCG 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 6, 37
BspQI GCTCTTC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 532
BssNI GRCGYC 1 cut(s) 516
Bst2UI CCWGG 2 cut(s) 47, 166
Bst6I CTCTTC 3 cut(s) 297, 355, 375
BstACI GRCGYC 1 cut(s) 516
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 2 cut(s) 9, 341
BstF5I GGATG 2 cut(s) 262, 572
BstFNI CGCG 1 cut(s) 127
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 129
BstMAI GTCTC 2 cut(s) 75, 523
BstMCI CGRYCG 1 cut(s) 118
BstNI CCWGG 2 cut(s) 47, 166
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 45, 164
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 273
BstV2I GAAGAC 1 cut(s) 426
Bsu36I CCTNAGG 1 cut(s) 341
BsuRI GGCC 1 cut(s) 95
BtsCI GGATG 2 cut(s) 262, 572
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 2 cut(s) 85, 129
Cfr13I GGNCC 1 cut(s) 581
CviAII CATG 4 cut(s) 299, 377, 445, 469
DdeI CTNAG 2 cut(s) 9, 341
DraI TTTAAA 1 cut(s) 312
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 3 cut(s) 297, 355, 375
EarI CTCTTC 3 cut(s) 297, 355, 375
EciI GGCGGA 1 cut(s) 41
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 581
Eco47III AGCGCT 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 573
Eco81I CCTNAGG 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 45, 164
EcoRV GATATC 1 cut(s) 157
EcoT22I ATGCAT 1 cut(s) 300
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 4 cut(s) 302, 380, 448, 472
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FatI CATG 4 cut(s) 298, 376, 444, 468
FblI GTMKAC 2 cut(s) 477, 513
Fnu4HI GCNGC 2 cut(s) 130, 262
FokI GGATG 2 cut(s) 269, 579
Fsp4HI GCNGC 2 cut(s) 130, 262
FspBI CTAG 1 cut(s) 585
GlaI GCGC 2 cut(s) 84, 128
GluI GCNGC 2 cut(s) 130, 262
GsuI CTGGAG 1 cut(s) 505
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 1 cut(s) 95
HapII CCGG 1 cut(s) 485
HhaI GCGC 2 cut(s) 85, 129
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 4 cut(s) 302, 380, 448, 472
Hin6I GCGC 2 cut(s) 83, 127
HinP1I GCGC 2 cut(s) 83, 127
HinfI GANTC 3 cut(s) 122, 458, 530
HpaII CCGG 1 cut(s) 485
Hpy166II GTNNAC 2 cut(s) 478, 514
Hpy188III TCNNGA 2 cut(s) 100, 119
Hpy8I GTNNAC 2 cut(s) 478, 514
Hpy99I CGWCG 3 cut(s) 101, 152, 550
HpyAV CCTTC 1 cut(s) 44
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 2 cut(s) 298, 392
HpyF3I CTNAG 2 cut(s) 9, 341
HpySE526I ACGT 1 cut(s) 516
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 4 cut(s) 302, 380, 448, 472
HspAI GCGC 2 cut(s) 83, 127
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 4 cut(s) 4, 41, 90, 139
LpnPI CCDG 8 cut(s) 18, 21, 32, 59, 151, 178, 498, 535
Lsp1109I GCAGC 1 cut(s) 273
LweI GCATC 1 cut(s) 273
MaeI CTAG 1 cut(s) 585
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 2 cut(s) 119, 413
MboII GAAGA 7 cut(s) 151, 221, 314, 362, 372, 431, 572
MfeI CAATTG 1 cut(s) 89
MluCI AATT 4 cut(s) 89, 313, 319, 607
MlyI GAGTC 3 cut(s) 116, 452, 539
MmeI TCCRAC 1 cut(s) 559
Mph1103I ATGCAT 1 cut(s) 300
MseI TTAA 3 cut(s) 287, 311, 503
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 2 cut(s) 47, 166
MunI CAATTG 1 cut(s) 89
MvaI CCWGG 2 cut(s) 47, 166
MvnI CGCG 1 cut(s) 127
NlaIII CATG 4 cut(s) 302, 380, 448, 472
NlaIV GGNNCC 2 cut(s) 6, 37
NmuCI GTSAC 2 cut(s) 119, 413
NsiI ATGCAT 1 cut(s) 300
NspI RCATGY 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 375
PfoI TCCNGGA 1 cut(s) 45
PkrI GCNGC 2 cut(s) 131, 263
PleI GAGTC 3 cut(s) 116, 452, 538
PpsI GAGTC 3 cut(s) 116, 452, 538
Psp6I CCWGG 2 cut(s) 45, 164
PspGI CCWGG 2 cut(s) 45, 164
PspN4I GGNNCC 2 cut(s) 6, 37
PspPI GGNCC 1 cut(s) 581
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 3 cut(s) 287, 311, 503
SatI GCNGC 2 cut(s) 130, 262
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 3 cut(s) 116, 452, 539
ScrFI CCNGG 2 cut(s) 47, 166
SetI ASST 9 cut(s) 21, 229, 276, 280, 390, 439, 519, 576, 586
SfaNI GCATC 1 cut(s) 273
SinI GGWCC 1 cut(s) 581
Sse9I AATT 4 cut(s) 89, 313, 319, 607
SsiI CCGC 5 cut(s) 26, 114, 130, 137, 374
SspMI CTAG 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 45, 164
TaiI ACGT 1 cut(s) 519
TaqI TCGA 3 cut(s) 99, 267, 329
TasI AATT 4 cut(s) 89, 313, 319, 607
TauI GCSGC 1 cut(s) 132
Tru1I TTAA 3 cut(s) 287, 311, 503
Tru9I TTAA 3 cut(s) 287, 311, 503
TseFI GTSAC 2 cut(s) 119, 413
TseI GCWGC 1 cut(s) 261
Tsp45I GTSAC 2 cut(s) 119, 413
TspDTI ATGAA 2 cut(s) 237, 315
TspGWI ACGGA 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 581
XceI RCATGY 1 cut(s) 380
XmiI GTMKAC 2 cut(s) 477, 513
XspI CTAG 1 cut(s) 585
ZraI GACGTC 1 cut(s) 517
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.