Rroxscaffold_4G00317140

pre-rRNA-processing protein TSR2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000787
Physical Location & Seq
Forward (+)
42238684 .. 42245147
6464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317140.1

Sequence Viewer

Length: 582 bp
ATGGAGCCTGAGAGAAAGCTAACGGCGGCGGCTGTGGCGGCGTTCCAAGAAGGGGTCGGGTTGGTTCTGTCGCGGTGGTCGGCGCTTCAATTGGCCGTCGAGAATGAGTGGGGTGGCCGTGAATCACGCCTCAAGGCCGAGCAACTGGTCTCTGATATCGTCTCCTGGGTCAATTTCTCCACCGAACCTCTTTATATAGATGATTTAGAAGAAATGCTCATTGAAGCTATGAGTTTTCTCAATACTGTGATAGAGGATGGTAGCATTGAGGAGGTAGCTGAAAAAATAATGATTATGCATGAAGAATGTTTGGATTGCAATTTCAAGTCTATTCAAAGCCTAAGGGAAGCCAATAGTCGAAGAGTTCCTACTCCTCATGTTAAACAGGTTGTCAATGATGAAGAAGACAGTGACGAGGATGATGATGGTACTGATCCTAGCATGAATAAAGATGATTCATCAAACATGATTGTGGACAAACCAGAGTCTCATGCAGTAGAGCCAAAGACCAAGCAGGCAGTCGAAGCGGAAGATGGTTGGGAGGTAGTCGGACCAAGAAGAAGTAGGGGTAAAAGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WGG

Protein Analysis

193

Amino Acids

21.69

Weight (kDa)

4.43

Isoelectric Point (pI)

66.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WGG PF10273 15 - 94 1.8e-24 Pre-rRNA-processing protein TSR2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 73
AciI CCGC 5 cut(s) 26, 29, 38, 73, 527
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 2 cut(s) 93, 115
AfaI GTAC 1 cut(s) 430
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 4 cut(s) 89, 224, 325, 335
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 3 cut(s) 19, 227, 278
AluI AGCT 3 cut(s) 19, 227, 278
Alw26I GTCTC 3 cut(s) 154, 166, 492
AlwI GGATC 1 cut(s) 428
AoxI GGCC 3 cut(s) 93, 115, 135
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 551
AvaII GGWCC 1 cut(s) 551
AxyI CCTNAGG 1 cut(s) 341
BarI GAAGNNNNNNTAC 2 cut(s) 352, 384
BbsI GAAGAC 1 cut(s) 411
BccI CCATC 3 cut(s) 251, 419, 527
BceAI ACGGC 3 cut(s) 39, 80, 102
BciT130I CCWGG 1 cut(s) 166
BcoDI GTCTC 3 cut(s) 154, 166, 492
BfaI CTAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 86
BisI GCNGC 3 cut(s) 27, 30, 39
BlsI GCNGC 3 cut(s) 28, 31, 40
Bme1390I CCNGG 1 cut(s) 166
Bme18I GGWCC 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 551
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 411
BpuEI CTTGAG 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse1I ACTGG 1 cut(s) 150
Bse21I CCTNAGG 1 cut(s) 341
BseBI CCWGG 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 2 cut(s) 262, 424
BseLI CCNNNNNNNGG 1 cut(s) 52
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 2 cut(s) 284, 363
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 3 cut(s) 95, 117, 137
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 3 cut(s) 154, 166, 492
BsmBI CGTCTC 1 cut(s) 166
BsnI GGCC 3 cut(s) 95, 117, 137
Bso31I GGTCTC 1 cut(s) 154
Bsp143I GATC 1 cut(s) 433
BspACI CCGC 5 cut(s) 26, 29, 38, 73, 527
BspANI GGCC 3 cut(s) 95, 117, 137
BspFNI CGCG 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 154
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 1 cut(s) 433
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 2 cut(s) 247, 410
Bst6I CTCTTC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 2 cut(s) 9, 341
BstF5I GGATG 2 cut(s) 262, 424
BstFNI CGCG 1 cut(s) 73
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 3 cut(s) 154, 166, 492
BstMBI GATC 1 cut(s) 433
BstMWI GCNNNNNNNGC 3 cut(s) 35, 38, 524
BstNI CCWGG 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 164
BstUI CGCG 1 cut(s) 73
BstV2I GAAGAC 1 cut(s) 411
Bsu36I CCTNAGG 1 cut(s) 341
BsuRI GGCC 3 cut(s) 95, 117, 137
BtsCI GGATG 2 cut(s) 262, 424
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 516
CfoI GCGC 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 551
Csp6I GTAC 1 cut(s) 429
CviAII CATG 5 cut(s) 299, 377, 442, 466, 491
CviQI GTAC 1 cut(s) 429
DdeI CTNAG 2 cut(s) 9, 341
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
EaeI YGGCCR 2 cut(s) 93, 115
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 154
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 551
Eco81I CCTNAGG 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 164
EcoRV GATATC 1 cut(s) 157
EcoT22I ATGCAT 1 cut(s) 300
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 5 cut(s) 302, 380, 445, 469, 494
FaiI YATR 9 cut(s) 195, 197, 230, 296, 300, 378, 443, 467, 492
FatI CATG 5 cut(s) 298, 376, 441, 465, 490
Fnu4HI GCNGC 3 cut(s) 27, 30, 39
FokI GGATG 2 cut(s) 269, 431
Fsp4HI GCNGC 3 cut(s) 27, 30, 39
FspBI CTAG 1 cut(s) 438
GlaI GCGC 1 cut(s) 84
GluI GCNGC 3 cut(s) 27, 30, 39
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 3 cut(s) 95, 117, 137
HhaI GCGC 1 cut(s) 85
Hin1II CATG 5 cut(s) 302, 380, 445, 469, 494
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HinfI GANTC 3 cut(s) 122, 455, 485
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 2 cut(s) 154, 551
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 2 cut(s) 247, 410
HpyCH4V TGCA 3 cut(s) 298, 318, 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 38, 524
HpyF3I CTNAG 2 cut(s) 9, 341
Hsp92II CATG 5 cut(s) 302, 380, 445, 469, 494
HspAI GCGC 1 cut(s) 83
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 21, 131, 151, 178, 371, 495, 500
MaeI CTAG 1 cut(s) 438
MaeIII GTNAC 1 cut(s) 410
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 7 cut(s) 221, 314, 372, 413, 416, 542, 570
MfeI CAATTG 1 cut(s) 89
MluCI AATT 4 cut(s) 89, 172, 319, 577
MlyI GAGTC 1 cut(s) 494
MmeI TCCRAC 1 cut(s) 529
MnlI CCTC 8 cut(s) 140, 198, 247, 262, 265, 384, 409, 535
Mph1103I ATGCAT 1 cut(s) 300
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 470
MspR9I CCNGG 1 cut(s) 166
MunI CAATTG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 166
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 3 cut(s) 35, 38, 524
NdeII GATC 1 cut(s) 433
NlaIII CATG 5 cut(s) 302, 380, 445, 469, 494
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 410
NsiI ATGCAT 1 cut(s) 300
PfeI GAWTC 2 cut(s) 122, 455
PkrI GCNGC 3 cut(s) 28, 31, 40
PleI GAGTC 1 cut(s) 493
PpsI GAGTC 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 164
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 551
RsaI GTAC 1 cut(s) 430
RsaNI GTAC 1 cut(s) 429
RseI CAYNNNNRTG 1 cut(s) 470
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 3 cut(s) 27, 30, 39
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 1 cut(s) 166
SetI ASST 7 cut(s) 21, 190, 229, 276, 280, 390, 546
SinI GGWCC 1 cut(s) 551
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 4 cut(s) 89, 172, 319, 577
SsiI CCGC 5 cut(s) 26, 29, 38, 73, 527
SspMI CTAG 1 cut(s) 438
StyD4I CCNGG 1 cut(s) 164
TaaI ACNGT 2 cut(s) 247, 410
TaqI TCGA 3 cut(s) 99, 358, 522
TasI AATT 4 cut(s) 89, 172, 319, 577
TauI GCSGC 3 cut(s) 29, 32, 41
TfiI GAWTC 2 cut(s) 122, 455
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 4 cut(s) 315, 414, 447, 458
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 551
XspI CTAG 1 cut(s) 438
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.