MD04G1217800.v1.1

pre-rRNA-processing protein TSR2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000787
Physical Location & Seq
Reverse (-)
30038582 .. 30040876
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1217800.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGAAGCTGAGAGAAAGCTGTCGGCAGATGCGGAGGCGATATTCAGAGAAGGCATCGGTCTGGTTCTGTCCCGGTGGTCGGCGCTCCAGTTGGCCGTCGACAACGAGTGGGGCGGCCGTGGCTCGCGCCAAAAAGCCGAGCAACTCGCCGCCGATGCCTTCTCGTGGTTCACTCAGTCTGCAGAGATTCTGTACATTGATGATTTGGAAGATATTCTCAATGAAGCTATGATTTCTCTCAATACAATGACAGAGGATGGCAGCATTGAGGAGGTGGCTGAAAAGTTAATGTTTATGTATGAAGACTGTTTGACTGGCAATTTCAATTCCGTTGAAAGTCTGAGAGAAGCCAATCATCGAAGAGTTGCCGTTCCTCATGTTAGACAAGACAATGAAGATGATGATGACGACGACGACAATGACAACGAGGAAAATGATGGTTCATCTAGCATGATGGTGGACACACCAGAGTCCCAGCCAAATTTGAATCCAGCAGACGCGACGAGCAGTAAACCAACTCCCAAGCCAGCAGCTAAAGCAGAAGATGGATGGGAAGTAGTTGGACCAAGACGTAATCGGGGTAAAAGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WGG

Protein Analysis

198

Amino Acids

21.97

Weight (kDa)

4.28

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WGG PF10273 15 - 94 2e-23 Pre-rRNA-processing protein TSR2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 99
AccII CGCG 2 cut(s) 127, 500
AciI CCGC 3 cut(s) 32, 114, 150
AcoI YGGCCR 2 cut(s) 93, 115
AcsI RAATTY 1 cut(s) 481
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 79, 165
AgsI TTSAA 3 cut(s) 325, 335, 487
AluBI AGCT 4 cut(s) 8, 19, 227, 533
AluI AGCT 4 cut(s) 8, 19, 227, 533
AoxI GGCC 2 cut(s) 93, 115
ApeKI GCWGC 2 cut(s) 261, 530
ApoI RAATTY 1 cut(s) 481
Asp700I GAANNNNTTC 1 cut(s) 213
AspLEI GCGC 2 cut(s) 85, 129
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 1 cut(s) 73
AvaII GGWCC 1 cut(s) 563
BauI CACGAG 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 273, 542
BccI CCATC 5 cut(s) 251, 431, 448, 539, 543
BceAI ACGGC 3 cut(s) 80, 102, 353
BcnI CCSGG 1 cut(s) 73
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 86
BisI GCNGC 4 cut(s) 115, 150, 262, 531
BlsI GCNGC 4 cut(s) 116, 151, 263, 532
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 563
BmrFI CCNGG 1 cut(s) 73
BmsI GCATC 3 cut(s) 19, 63, 145
BpiI GAAGAC 1 cut(s) 309
BpmI CTGGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 165
Bse1I ACTGG 2 cut(s) 88, 319
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 2 cut(s) 262, 554
BseLI CCNNNNNNNGG 2 cut(s) 79, 165
BseMII CTCAG 2 cut(s) 188, 332
BseNI ACTGG 2 cut(s) 88, 319
BseRI GAGGAG 1 cut(s) 284
BseX3I CGGCCG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 273, 542
BseYI CCCAGC 1 cut(s) 474
Bsh1236I CGCG 2 cut(s) 127, 500
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 95, 117
BsiEI CGRYCG 1 cut(s) 118
BsiSI CCGG 1 cut(s) 73
BslFI GGGAC 2 cut(s) 55, 457
BslI CCNNNNNNNGG 2 cut(s) 79, 165
BsmFI GGGAC 2 cut(s) 55, 457
BsnI GGCC 2 cut(s) 95, 117
Bsp1407I TGTACA 1 cut(s) 192
BspACI CCGC 3 cut(s) 32, 114, 150
BspANI GGCC 2 cut(s) 95, 117
BspCNI CTCAG 2 cut(s) 187, 333
BspFNI CGCG 2 cut(s) 127, 500
BspMAI CTGCAG 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 192
BsrI ACTGG 2 cut(s) 88, 319
BssECI CCNNGG 1 cut(s) 118
BssSI CACGAG 1 cut(s) 163
Bst2BI CACGAG 1 cut(s) 163
Bst4CI ACNGT 1 cut(s) 308
Bst6I CTCTTC 1 cut(s) 355
BstAUI TGTACA 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 125, 528
BstDEI CTNAG 3 cut(s) 9, 174, 341
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 2 cut(s) 262, 554
BstFNI CGCG 2 cut(s) 127, 500
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 129
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 3 cut(s) 120, 155, 536
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 2 cut(s) 127, 500
BstV1I GCAGC 2 cut(s) 273, 542
BstV2I GAAGAC 1 cut(s) 309
BstZI CGGCCG 1 cut(s) 115
BsuRI GGCC 2 cut(s) 95, 117
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 262, 554
Cac8I GCNNGC 2 cut(s) 125, 528
CfoI GCGC 2 cut(s) 85, 129
Cfr13I GGNCC 1 cut(s) 563
CseI GACGC 1 cut(s) 506
Csp6I GTAC 1 cut(s) 193
CviAII CATG 2 cut(s) 377, 451
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 3 cut(s) 9, 174, 341
EaeI YGGCCR 2 cut(s) 93, 115
EagI CGGCCG 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
EclXI CGGCCG 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 563
Eco52I CGGCCG 1 cut(s) 115
FaeI CATG 2 cut(s) 380, 454
FaiI YATR 5 cut(s) 230, 296, 300, 378, 452
FaqI GGGAC 2 cut(s) 55, 457
FatI CATG 2 cut(s) 376, 450
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 4 cut(s) 115, 150, 262, 531
FokI GGATG 2 cut(s) 269, 561
Fsp4HI GCNGC 4 cut(s) 115, 150, 262, 531
FspBI CTAG 1 cut(s) 447
GlaI GCGC 2 cut(s) 84, 128
GluI GCNGC 4 cut(s) 115, 150, 262, 531
GsaI CCCAGC 1 cut(s) 478
GsuI CTGGAG 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 2 cut(s) 95, 117
HapII CCGG 1 cut(s) 73
HgaI GACGC 1 cut(s) 506
HhaI GCGC 2 cut(s) 85, 129
Hin1II CATG 2 cut(s) 380, 454
Hin6I GCGC 2 cut(s) 83, 127
HinP1I GCGC 2 cut(s) 83, 127
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 3 cut(s) 187, 470, 487
HpaII CCGG 1 cut(s) 73
Hpy166II GTNNAC 4 cut(s) 100, 171, 460, 512
Hpy188I TCNGA 2 cut(s) 47, 342
Hpy8I GTNNAC 4 cut(s) 100, 171, 460, 512
Hpy99I CGWCG 4 cut(s) 101, 413, 416, 505
HpyAV CCTTC 2 cut(s) 44, 169
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 155, 536
HpyF3I CTNAG 3 cut(s) 9, 174, 341
HpySE526I ACGT 1 cut(s) 571
Hsp92II CATG 2 cut(s) 380, 454
HspAI GCGC 2 cut(s) 83, 127
LmnI GCTCC 1 cut(s) 90
LpnPI CCDG 8 cut(s) 47, 86, 101, 300, 480, 488, 504, 540
Lsp1109I GCAGC 2 cut(s) 273, 542
LweI GCATC 3 cut(s) 19, 63, 145
MaeI CTAG 1 cut(s) 447
MaeII ACGT 1 cut(s) 571
MboII GAAGA 5 cut(s) 221, 314, 372, 407, 554
MluCI AATT 4 cut(s) 319, 325, 481, 589
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 6 cut(s) 28, 247, 262, 265, 384, 421
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 1 cut(s) 287
MslI CAYNNNNRTG 1 cut(s) 455
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 1 cut(s) 73
MvnI CGCG 2 cut(s) 127, 500
MwoI GCNNNNNNNGC 3 cut(s) 120, 155, 536
NciI CCSGG 1 cut(s) 73
NlaIII CATG 2 cut(s) 380, 454
NmeAIII GCCGAG 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 187, 487
PkrI GCNGC 4 cut(s) 116, 151, 263, 532
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PspFI CCCAGC 1 cut(s) 474
PspPI GGNCC 1 cut(s) 563
PstI CTGCAG 1 cut(s) 184
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 455
SalI GTCGAC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 4 cut(s) 115, 150, 262, 531
Sau96I GGNCC 1 cut(s) 563
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 1 cut(s) 73
SetI ASST 6 cut(s) 10, 21, 229, 276, 535, 574
SfaNI GCATC 3 cut(s) 19, 63, 145
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 455
Sse9I AATT 4 cut(s) 319, 325, 481, 589
SsiI CCGC 3 cut(s) 32, 114, 150
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 1 cut(s) 71
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 1 cut(s) 574
TaqI TCGA 2 cut(s) 99, 358
TaqII GACCGA 1 cut(s) 47
TasI AATT 4 cut(s) 319, 325, 481, 589
TatI WGTACW 1 cut(s) 192
TauI GCSGC 2 cut(s) 117, 152
TfiI GAWTC 2 cut(s) 187, 487
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TseI GCWGC 2 cut(s) 261, 530
TspDTI ATGAA 4 cut(s) 237, 315, 408, 432
TspGWI ACGGA 1 cut(s) 319
VpaK11BI GGWCC 1 cut(s) 563
XapI RAATTY 1 cut(s) 481
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.