MD12G1235200.v1.1

pre-rRNA-processing protein TSR2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000787
Physical Location & Seq
Reverse (-)
30995418 .. 30996173
756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1235200.v1.1.491

Sequence Viewer

Length: 294 bp
ATGGAAGGTGAGAGAAAGCTGTCGGCGGAGGCGGTGGCGATATTCAGAGAAGGCATAGGGTTGGTTTTGTCCCGGTGGTCGGCGCTCCAATTGGCCGTCGACAACGAGTGGGGCGGCCGTGGCTCGCGCCAAAAAGCGGAGCAACTAGCGGCCGATGCCTTCTCCTGGTTCACTCAGTCTGCAGAGATTCTATACATTGATGATTTGGAAGATATTCTCAATGAAGCTATGATTTCTCTCAATACAATGACAGAGGATGGCAGCATTGAGGAGGTAATGCTCTTCAATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WGG

Protein Analysis

98

Amino Acids

10.8

Weight (kDa)

4.13

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WGG PF10273 15 - 92 2.7e-23 Pre-rRNA-processing protein TSR2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 99
AccII CGCG 1 cut(s) 127
AciI CCGC 5 cut(s) 26, 32, 114, 137, 149
AcoI YGGCCR 3 cut(s) 93, 115, 150
AfiI CCNNNNNNNGG 3 cut(s) 79, 136, 165
AgsI TTSAA 1 cut(s) 286
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 2 cut(s) 19, 227
AluI AGCT 2 cut(s) 19, 227
AoxI GGCC 3 cut(s) 93, 115, 150
ApeKI GCWGC 1 cut(s) 261
Asp700I GAANNNNTTC 1 cut(s) 213
AspLEI GCGC 2 cut(s) 85, 129
AsuC2I CCSGG 1 cut(s) 73
AsuHPI GGTGA 1 cut(s) 20
BbvI GCAGC 1 cut(s) 273
BccI CCATC 1 cut(s) 251
BceAI ACGGC 2 cut(s) 80, 102
BciT130I CCWGG 1 cut(s) 166
BcnI CCSGG 1 cut(s) 73
BfaI CTAG 1 cut(s) 146
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 86
BisI GCNGC 3 cut(s) 115, 150, 262
BlsI GCNGC 3 cut(s) 116, 151, 263
Bme1390I CCNGG 2 cut(s) 73, 166
BmrFI CCNGG 2 cut(s) 73, 166
BmsI GCATC 1 cut(s) 145
BpuMI CCSGG 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 136, 165
BseBI CCWGG 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 3 cut(s) 79, 136, 165
BseMII CTCAG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 284
BseX3I CGGCCG 2 cut(s) 115, 150
BseXI GCAGC 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 127
Bsh1285I CGRYCG 2 cut(s) 118, 153
BshFI GGCC 3 cut(s) 95, 117, 152
BsiEI CGRYCG 2 cut(s) 118, 153
BsiSI CCGG 1 cut(s) 73
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 3 cut(s) 79, 136, 165
BsmFI GGGAC 1 cut(s) 55
BsnI GGCC 3 cut(s) 95, 117, 152
BspACI CCGC 5 cut(s) 26, 32, 114, 137, 149
BspANI GGCC 3 cut(s) 95, 117, 152
BspCNI CTCAG 1 cut(s) 187
BspFNI CGCG 1 cut(s) 127
BspMAI CTGCAG 1 cut(s) 184
BspQI GCTCTTC 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 1 cut(s) 127
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 129
BstMCI CGRYCG 2 cut(s) 118, 153
BstMWI GCNNNNNNNGC 2 cut(s) 120, 155
BstNI CCWGG 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 71, 164
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 273
BstZI CGGCCG 2 cut(s) 115, 150
BsuRI GGCC 3 cut(s) 95, 117, 152
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 262
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 2 cut(s) 85, 129
CviJI RGCY 6 cut(s) 19, 95, 117, 123, 152, 227
CviKI_1 RGCY 6 cut(s) 19, 95, 117, 123, 152, 227
DdeI CTNAG 1 cut(s) 174
EaeI YGGCCR 3 cut(s) 93, 115, 150
EagI CGGCCG 2 cut(s) 115, 150
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
EciI GGCGGA 1 cut(s) 41
EclXI CGGCCG 2 cut(s) 115, 150
Eco52I CGGCCG 2 cut(s) 115, 150
EcoRII CCWGG 1 cut(s) 164
FaiI YATR 4 cut(s) 56, 193, 230, 292
FaqI GGGAC 1 cut(s) 55
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 3 cut(s) 115, 150, 262
FokI GGATG 1 cut(s) 269
Fsp4HI GCNGC 3 cut(s) 115, 150, 262
FspBI CTAG 1 cut(s) 146
GlaI GCGC 2 cut(s) 84, 128
GluI GCNGC 3 cut(s) 115, 150, 262
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 3 cut(s) 95, 117, 152
HapII CCGG 1 cut(s) 73
HhaI GCGC 2 cut(s) 85, 129
Hin6I GCGC 2 cut(s) 83, 127
HinP1I GCGC 2 cut(s) 83, 127
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 1 cut(s) 187
HpaII CCGG 1 cut(s) 73
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 2 cut(s) 100, 171
Hpy188I TCNGA 1 cut(s) 47
Hpy8I GTNNAC 2 cut(s) 100, 171
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 44, 169
HpyCH4V TGCA 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 2 cut(s) 120, 155
HpyF3I CTNAG 1 cut(s) 174
HspAI GCGC 2 cut(s) 83, 127
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 2 cut(s) 90, 139
LpnPI CCDG 3 cut(s) 86, 151, 178
Lsp1109I GCAGC 1 cut(s) 273
LweI GCATC 1 cut(s) 145
MaeI CTAG 1 cut(s) 146
MboII GAAGA 2 cut(s) 221, 274
MfeI CAATTG 1 cut(s) 89
MluCI AATT 2 cut(s) 89, 286
MnlI CCTC 4 cut(s) 22, 247, 262, 265
MroXI GAANNNNTTC 1 cut(s) 213
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 2 cut(s) 73, 166
MunI CAATTG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 166
MvnI CGCG 1 cut(s) 127
MwoI GCNNNNNNNGC 2 cut(s) 120, 155
NciI CCSGG 1 cut(s) 73
PciSI GCTCTTC 1 cut(s) 287
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 187
PkrI GCNGC 3 cut(s) 116, 151, 263
Psp6I CCWGG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 164
PstI CTGCAG 1 cut(s) 184
SalI GTCGAC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 287
SatI GCNGC 3 cut(s) 115, 150, 262
ScrFI CCNGG 2 cut(s) 73, 166
SetI ASST 4 cut(s) 10, 21, 229, 276
SfaNI GCATC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 180
SgeI CNNG 9 cut(s) 84, 85, 118, 131, 136, 138, 158, 177, 178
Sse9I AATT 2 cut(s) 89, 286
SsiI CCGC 5 cut(s) 26, 32, 114, 137, 149
SspMI CTAG 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 71, 164
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 89, 286
TauI GCSGC 2 cut(s) 117, 152
TfiI GAWTC 1 cut(s) 187
TseI GCWGC 1 cut(s) 261
TspDTI ATGAA 2 cut(s) 237, 279
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.