MD12G1235100.v1.1

pre-rRNA-processing protein TSR2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000787
Physical Location & Seq
Reverse (-)
30993386 .. 30995416
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1235100.v1.1.491

Sequence Viewer

Length: 534 bp
ATGGGGGTAAGACTAACATTCACCTCTCCCAGAGCTTGCGTAAAGCGGGAGCCTTGTGCACAAGGGGCAGCACATGGCGCTAGTCAACTTGATTTAGCGGAAGCCTGGCGATTTTCCTTTGATAGTTATCATTTATGTATGTCTGCATATTTTTGCTGCAATTTGGCTAGTTTTTTTTGTATCGGGGATGCGGTTTTTCAGTATTGGTTTTCTGTGGCTGAAAAGTTAATGTTTATGTATGATGACTGTTTGACTGACAATTTCAATTCTGTTGAAAGTTTGAGAGATGCCAATCGTCGAAGAGTTGCCGTTCCTCATGTTAGACAAGAGAATGAAGATGACGACGATGATGATGATGATGACAACGAGGAAACTGATGGTTCATCAAACATGATGGTGGACATACCAGAGTCCCAGCCAAATTTGGATCCAGCAGACGCGACGAGCAGTAAACCAACTCCCAAGCCAGCAGCTAAAACAGAAGATGGATGGGAAGTAGTTGGACCGAGACATAATCGGGGTAAAAGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WGG

Protein Analysis

178

Amino Acids

19.92

Weight (kDa)

4.54

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 440
AciI CCGC 3 cut(s) 46, 98, 191
AclWI GGATC 2 cut(s) 422, 435
AcsI RAATTY 1 cut(s) 421
AgsI TTSAA 2 cut(s) 265, 275
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 2 cut(s) 35, 473
AluI AGCT 2 cut(s) 35, 473
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 1 cut(s) 502
Alw44I GTGCAC 1 cut(s) 57
AlwI GGATC 2 cut(s) 422, 435
ApaLI GTGCAC 1 cut(s) 57
ApeKI GCWGC 3 cut(s) 68, 156, 470
ApoI RAATTY 1 cut(s) 421
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 503
AsuHPI GGTGA 1 cut(s) 13
AvaII GGWCC 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 61
BamHI GGATCC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 3 cut(s) 80, 143, 482
BccI CCATC 4 cut(s) 371, 388, 479, 483
BceAI ACGGC 1 cut(s) 293
BciT130I CCWGG 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 502
BfaI CTAG 2 cut(s) 81, 168
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 3 cut(s) 69, 157, 471
BlsI GCNGC 3 cut(s) 70, 158, 472
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 1 cut(s) 503
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 2 cut(s) 51, 429
BmrFI CCNGG 1 cut(s) 106
BmsI GCATC 2 cut(s) 178, 277
BsaBI GATNNNNATC 2 cut(s) 126, 291
Bse8I GATNNNNATC 2 cut(s) 126, 291
BseBI CCWGG 1 cut(s) 106
BseGI GGATG 2 cut(s) 193, 494
BseJI GATNNNNATC 2 cut(s) 126, 291
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 3 cut(s) 80, 143, 482
BseYI CCCAGC 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 440
BsiHKAI GWGCWC 1 cut(s) 61
BslFI GGGAC 1 cut(s) 397
BsmAI GTCTC 1 cut(s) 502
BsmFI GGGAC 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 1 cut(s) 427
BspACI CCGC 3 cut(s) 46, 98, 191
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 2 cut(s) 51, 429
BspPI GGATC 2 cut(s) 422, 435
BssMI GATC 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 1 cut(s) 295
BstC8I GCNNGC 2 cut(s) 37, 468
BstF5I GGATG 2 cut(s) 193, 494
BstFNI CGCG 1 cut(s) 440
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 1 cut(s) 430
BstMAI GTCTC 1 cut(s) 502
BstMBI GATC 1 cut(s) 427
BstMWI GCNNNNNNNGC 2 cut(s) 65, 77
BstNI CCWGG 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 104
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 80, 143, 482
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BtsCI GGATG 2 cut(s) 193, 494
Cac8I GCNNGC 2 cut(s) 37, 468
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 503
CseI GACGC 1 cut(s) 446
CviAII CATG 3 cut(s) 74, 317, 391
CviJI RGCY 8 cut(s) 35, 52, 104, 167, 218, 418, 466, 473
CviKI_1 RGCY 8 cut(s) 35, 52, 104, 167, 218, 418, 466, 473
DpnI GATC 1 cut(s) 429
DpnII GATC 1 cut(s) 427
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 104
FaeI CATG 3 cut(s) 77, 320, 394
FaqI GGGAC 1 cut(s) 397
FatI CATG 3 cut(s) 73, 316, 390
FauI CCCGC 1 cut(s) 39
Fnu4HI GCNGC 3 cut(s) 69, 157, 471
FokI GGATG 2 cut(s) 200, 501
Fsp4HI GCNGC 3 cut(s) 69, 157, 471
FspBI CTAG 2 cut(s) 81, 168
GlaI GCGC 1 cut(s) 79
GluI GCNGC 3 cut(s) 69, 157, 471
GsaI CCCAGC 1 cut(s) 418
HaeII RGCGCY 1 cut(s) 81
HgaI GACGC 1 cut(s) 446
HhaI GCGC 1 cut(s) 80
Hin1II CATG 3 cut(s) 77, 320, 394
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 86
HindII GTYRAC 1 cut(s) 86
HinfI GANTC 1 cut(s) 410
HphI GGTGA 1 cut(s) 13
Hpy166II GTNNAC 4 cut(s) 59, 86, 400, 452
Hpy8I GTNNAC 4 cut(s) 59, 86, 400, 452
Hpy99I CGWCG 3 cut(s) 300, 347, 445
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 3 cut(s) 59, 146, 159
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 77
Hsp92II CATG 3 cut(s) 77, 320, 394
HspAI GCGC 1 cut(s) 78
Kzo9I GATC 1 cut(s) 427
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 7 cut(s) 43, 91, 118, 420, 428, 444, 480
Lsp1109I GCAGC 3 cut(s) 80, 143, 482
LweI GCATC 2 cut(s) 178, 277
MaeI CTAG 2 cut(s) 81, 168
MalI GATC 1 cut(s) 429
MboI GATC 1 cut(s) 427
MboII GAAGA 3 cut(s) 312, 347, 494
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 5 cut(s) 160, 259, 265, 421, 529
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 3 cut(s) 34, 324, 361
MseI TTAA 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 65, 77
NdeII GATC 1 cut(s) 427
NlaIII CATG 3 cut(s) 77, 320, 394
NlaIV GGNNCC 2 cut(s) 51, 429
PkrI GCNGC 3 cut(s) 70, 158, 472
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 104
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 2 cut(s) 51, 429
PspPI GGNCC 1 cut(s) 503
PsuI RGATCY 1 cut(s) 427
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 3 cut(s) 69, 157, 471
Sau3AI GATC 1 cut(s) 427
Sau96I GGNCC 1 cut(s) 503
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 1 cut(s) 61
SetI ASST 3 cut(s) 26, 37, 475
SfaNI GCATC 2 cut(s) 178, 277
SinI GGWCC 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 5 cut(s) 160, 259, 265, 421, 529
SsiI CCGC 3 cut(s) 46, 98, 191
SspMI CTAG 2 cut(s) 81, 168
StyD4I CCNGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 248
TaqI TCGA 1 cut(s) 298
TaqII GACCGA 1 cut(s) 520
TasI AATT 5 cut(s) 160, 259, 265, 421, 529
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TseI GCWGC 3 cut(s) 68, 156, 470
TspDTI ATGAA 2 cut(s) 348, 372
VneI GTGCAC 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 503
XapI RAATTY 1 cut(s) 421
XspI CTAG 2 cut(s) 81, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.