Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000221

Physical Location & Seq

Genomic Coordinates Reverse (-)

10112513 .. 10121473

Gene Structure 8961 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1131300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGAGTATTGCCGATTCACCACTACACTCGTCTAGCAGTGACGACTTTGTCGGGTTTCTTGAGAGTGCCCTGGATTCTGGTTCTCCGGGGTCATCACCGGATGAAGAAGCTGATGATGACAGTGACGTCGAGATTGATAGTAATAGTGCAAAGAGGCGTAAGGTAGAGTTTGGAAGCACTGAGGAGATTCCTGGGTCGACTTCAGAAATTTTCGTAGAAGAACTCTCAGAAGCATCCATGAAGGAGGACATTTGTACTCATCCTGGTTCATTTGGAGATATGTGTATCGTCTGTGGGCAGCCGGTGGATGACAAGTCTAGTGTGATATTTGGGTACATACACAAGGGATTAAGGCTTAATAATGATGAAATTGACCGGTTACGCAAAACAGACATTAAGAAGTCGTTGCACCATAAGAAGCTTTACTTGGTCCTTGATCTAGATCACACACTGCTTAATTCTACCCATCTGAATCATATGTCAGCCGAAGAGGAATATTTACATAGTCAAATAGATTCTCTGCAAGATGTTTCAAAAGGTAGCCTATTCAGAGTAGACATTATGCATATGATGACCAAGTTGAGGCCCTTTGTTAGGACATTTTTAAAAGAGGCCAGTGAAATGTTCGAGATGTACATATACACAATGGGCGAACGAGCTTATGCGCTGGAAATGGCTAGGCTGCTTGATCCGAGGAAAGAGTACTTTGGGGATAGGGTTATATCACGTGATGATGGTACCCAAAAACATCAAAAAGGTCTAGATATTGTGCTTGGACAAGATAATGCAGTTGTGATCCTTGACGATACAGAAAATGCCTGGACAAAGCACAAGGACAATTTAATATTGATGGAAAGATATCATTTCTTCAGTTCAAGCTGTAAACAATTTGGGTTTCGCTGTAAATCCCTTTCTGAGTTGAGAAGTGACGAAAGTGAACCTGAAGGAGCTCTTGCAACTGTTCTTGAAGTTCTTAAGCGAGTCCACAACATGTTCTTTCATGAATTGGAGGATAATCTTGTTGAAAGAGATGTGAGACAGGTGTTGAAAACTGTTAAGAAGGATGTGTTAAAGGGGTGTAAAATTGTTTTCAGCCACGAATTTCCTTCGAATGTCGATGCTGAGAATCAACCTCTCTGGAAGATGGCAGAGCAGTTAGGAGCCTCTTGTTCGACACAAGTGGACCCATCGGTGACACATGTGGTTTCAGGAGATGCTGGCACAGAGAAGTCTAAATGGGCGGTCAAAGAAAAGAAGTTTTTAGTTAATAAACAGTGGATTGAAGCTGCAAACTATATGTGGCAAAAGCAAGCTGAAGAGAAGTTCCCAGTCAAGCAAATGAAAGCCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008022 GO:0008150 GO:0008152 GO:0008420 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016311 GO:0016591 GO:0016787 GO:0016788 GO:0016791 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070940 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT NIF PTCB-BRCT

Protein Analysis

451

Amino Acids

51.17

Weight (kDa)

5.25

Isoelectric Point (pI)

49.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	142 - 289	1e-27	NLI interacting factor-like phosphatase
BRCT	PF00533	354 - 428	1.1e-08	BRCA1 C Terminus (BRCT) domain
PTCB-BRCT	PF12738	375 - 426	8.4e-06	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58003 AT5G58003 AT5G58003
fragaria_vesca	FvH4_1g01060 FvH4_1g01060 FvH4_1g01060 FvH4_1g01060 FvH4_2g05941 FvH4_7g02340 FvH4_7g02631 FvH4_7g02801 FvH4_7g07841 FvH4_7g08260
malus_domestica	MD09G1131300.v1.1
prunus_persica	Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1
pyrus_communis	pycom09g05350
rosa_chinensis	RchiOBHm_Chr1g0324741 RchiOBHm_Chr2g0085821 RchiOBHm_Chr2g0147611 RchiOBHm_Chr4g0392421 RchiOBHm_Chr6g0254001 RchiOBHm_Chr6g0256111 RchiOBHm_Chr6g0256201 RchiOBHm_Chr6g0256301 RchiOBHm_Chr6g0257451 RchiOBHm_Chr6g0267911 RchiOBHm_Chr6g0276921 RchiOBHm_Chr7g0234511 RchiOBHm_Chr7g0234521 RchiOBHm_Chr7g0234531 RchiOBHm_Chr7g0234711 RchiOBHm_Chr7g0234721 RchiOBHm_Chr7g0234801 RchiOBHm_Chr7g0234841 RchiOBHm_Chr7g0234851
rosa_laevigata	RLG00000001210 RLG00000001218 RLG00000013348 RLG00000013351 RLG00000013354 RLG00000013969 RLG00000014699 RLG00000014794 RLG00000014949 RLG00000015721 RLG00000030176 RLG00000030180 RLG00000030183
rosa_multiflora	Rmu_sc0000434.1_g000006 Rmu_sc0001716.1_g000020 Rmu_sc0001800.1_g000011 Rmu_sc0001913.1_g000023 Rmu_sc0003301.1_g000015 Rmu_sc0003882.1_g000010 Rmu_sc0005080.1_g000013 Rmu_sc0006724.1_g000007 Rmu_sc0006759.1_g000001 Rmu_sc0006859.1_g000001 Rmu_sc0007217.1_g000009 Rmu_sc0010500.1_g000001 Rmu_sc0015353.1_g000005 Rmu_sc0017253.1_g000006 Rmu_sc0021133.1_g000001 Rmu_sc0033202.1_g000001
rosa_roxburghii	Rroxscaffold_2G00155020 Rroxscaffold_4G00325270 Rroxscaffold_7G00200340 Rroxscaffold_7G00211310
rosa_rugosa	Rorug01G0046400 Rorug01G0463600 Rorug05G0571900 Rorug05G0572300 Rorug06G0034600 Rorug07G0285100 Rorug07G0285400 Rorug07G0285500 Rorug07G0285600
rosa_samantha	Rh1AG063200 Rh1AG063800 Rh1DG069200 Rh1DG070400 Rh2AG012600 Rh2BG013300 Rh2DG015000 Rh4AG042600 Rh4BG037500 Rh4CG045400 Rh4CG045600 Rh6AG065400 Rh6AG082000 Rh6AG082100 Rh6AG089900 Rh6AG156900 Rh6AG157000 Rh6AG157200 Rh6AG214900 Rh6AG215000 Rh6AG215200 Rh6AG291000 Rh6BG058300 Rh6BG074200 Rh6BG074300 Rh6BG081300 Rh6BG158100 Rh6BG158400 Rh6BG158700 Rh6BG219800 Rh6CG058400 Rh6CG071100 Rh6CG078000 Rh6CG157400 Rh6CG158100 Rh6CG222400 Rh6DG055200 Rh6DG068500 Rh6DG073700 Rh6DG143300 Rh6DG143700 Rh6DG144100 Rh6DG212600 Rh7AG440200 Rh7AG440300 Rh7BG413100 Rh7CG461400 Rh7CG461500 Rh7CG461900 Rh7CG462000 Rh7DG429900
rosa_wichuraiana	Rw1G005350 Rw2G001120 Rw4G003330 Rw6G005760 Rw6G007140 Rw6G007840 Rw6G013630 Rw6G013660 Rw6G018800 Rw6G025060 Rw7G036540 Rw7G036580 Rw7G036600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	47, 125
AatII	GACGTC	1 cut(s)	129
Acc65I	GGTACC	1 cut(s)	737
AccB1I	GGYRCC	1 cut(s)	737
AccI	GTMKAC	2 cut(s)	197, 555
AciI	CCGC	1 cut(s)	1241
AclWI	GGATC	2 cut(s)	683, 790
AcsI	RAATTY	2 cut(s)	207, 1100
AcuI	CTGAAG	4 cut(s)	186, 853, 963, 1335
AcvI	CACGTG	1 cut(s)	728
AcyI	GRCGYC	1 cut(s)	126
AfaI	GTAC	5 cut(s)	256, 335, 635, 704, 739
AfiI	CCNNNNNNNGG	2 cut(s)	582, 594
AflII	CTTAAG	1 cut(s)	974
AflIII	ACRYGT	2 cut(s)	990, 1198
AgeI	ACCGGT	1 cut(s)	375
AgsI	TTSAA	6 cut(s)	534, 876, 968, 1025, 1048, 1283
AjnI	CCWGG	4 cut(s)	69, 190, 262, 818
AjuI	GAANNNNNNNTTGG	2 cut(s)	410, 442
AluBI	AGCT	7 cut(s)	110, 421, 659, 879, 950, 1286, 1313
AluI	AGCT	7 cut(s)	110, 421, 659, 879, 950, 1286, 1313
Alw21I	GWGCWC	1 cut(s)	952
Alw26I	GTCTC	1 cut(s)	1030
AlwI	GGATC	2 cut(s)	683, 790
AoxI	GGCC	2 cut(s)	584, 612
ApeKI	GCWGC	3 cut(s)	298, 682, 1286
ApoI	RAATTY	2 cut(s)	207, 1100
AsiGI	ACCGGT	1 cut(s)	375
Asp718I	GGTACC	1 cut(s)	737
AspLEI	GCGC	1 cut(s)	667
AspS9I	GGNCC	3 cut(s)	430, 585, 1183
AsuC2I	CCSGG	1 cut(s)	87
AsuHPI	GGTGA	3 cut(s)	9, 87, 1204
AsuII	TTCGAA	1 cut(s)	1109
AvaII	GGWCC	2 cut(s)	430, 1183
BaeGI	GKGCMC	1 cut(s)	70
BanI	GGYRCC	1 cut(s)	737
BanII	GRGCYC	1 cut(s)	952
BbrPI	CACGTG	1 cut(s)	728
Bbv12I	GWGCWC	1 cut(s)	952
BbvI	GCAGC	3 cut(s)	310, 669, 1273
BccI	CCATC	5 cut(s)	474, 728, 844, 1138, 1195
BciT130I	CCWGG	4 cut(s)	71, 192, 264, 820
BcnI	CCSGG	1 cut(s)	87
BcoDI	GTCTC	1 cut(s)	1030
BfaI	CTAG	5 cut(s)	33, 318, 440, 678, 761
BfrI	CTTAAG	1 cut(s)	974
BisI	GCNGC	3 cut(s)	299, 683, 1287
BlsI	GCNGC	3 cut(s)	300, 684, 1288
BmcAI	AGTACT	1 cut(s)	704
Bme1390I	CCNGG	5 cut(s)	71, 87, 192, 264, 820
Bme18I	GGWCC	2 cut(s)	430, 1183
BmgT120I	GGNCC	3 cut(s)	430, 585, 1183
BmiI	GGNNCC	3 cut(s)	739, 1162, 1185
BmrFI	CCNGG	5 cut(s)	71, 87, 192, 264, 820
BmrI	ACTGGG	1 cut(s)	1322
BmsI	GCATC	3 cut(s)	242, 1108, 1204
BmuI	ACTGGG	1 cut(s)	1322
Bpu14I	TTCGAA	1 cut(s)	1109
BpuEI	CTTGAG	1 cut(s)	80
BpuMI	CCSGG	1 cut(s)	87
BsaAI	YACGTR	1 cut(s)	728
BsaBI	GATNNNNATC	1 cut(s)	441
BsaHI	GRCGYC	1 cut(s)	126
BsaJI	CCNNGG	4 cut(s)	69, 86, 191, 692
BsaWI	WCCGGW	2 cut(s)	97, 375
Bsc4I	CCNNNNNNNGG	2 cut(s)	582, 594
Bse118I	RCCGGY	2 cut(s)	301, 375
Bse1I	ACTGG	2 cut(s)	615, 1328
Bse8I	GATNNNNATC	1 cut(s)	441
BseBI	CCWGG	4 cut(s)	71, 192, 264, 820
BseDI	CCNNGG	4 cut(s)	69, 86, 191, 692
BseGI	GGATG	5 cut(s)	106, 233, 259, 313, 1069
BseJI	GATNNNNATC	1 cut(s)	441
BseLI	CCNNNNNNNGG	2 cut(s)	582, 594
BseMII	CTCAG	4 cut(s)	171, 240, 906, 1113
BseNI	ACTGG	2 cut(s)	615, 1328
BseRI	GAGGAG	1 cut(s)	197
BseSI	GKGCMC	1 cut(s)	70
BseXI	GCAGC	3 cut(s)	310, 669, 1273
BshFI	GGCC	2 cut(s)	586, 614
BshNI	GGYRCC	1 cut(s)	737
BshTI	ACCGGT	1 cut(s)	375
BsiHKAI	GWGCWC	1 cut(s)	952
BsiSI	CCGG	4 cut(s)	86, 98, 302, 376
BslI	CCNNNNNNNGG	2 cut(s)	582, 594
BsmAI	GTCTC	1 cut(s)	1030
BsnI	GGCC	2 cut(s)	586, 614
Bsp119I	TTCGAA	1 cut(s)	1109
Bsp1286I	GDGCHC	2 cut(s)	70, 952
Bsp1407I	TGTACA	1 cut(s)	633
Bsp143I	GATC	4 cut(s)	436, 442, 688, 795
BspACI	CCGC	1 cut(s)	1241
BspANI	GGCC	2 cut(s)	586, 614
BspCNI	CTCAG	4 cut(s)	172, 239, 907, 1114
BspHI	TCATGA	1 cut(s)	1000
BspLI	GGNNCC	3 cut(s)	739, 1162, 1185
BspPI	GGATC	2 cut(s)	683, 790
BspT104I	TTCGAA	1 cut(s)	1109
BspT107I	GGYRCC	1 cut(s)	737
BspTI	CTTAAG	1 cut(s)	974
BsrFI	RCCGGY	2 cut(s)	301, 375
BsrGI	TGTACA	1 cut(s)	633
BsrI	ACTGG	2 cut(s)	615, 1328
BssAI	RCCGGY	2 cut(s)	301, 375
BssECI	CCNNGG	4 cut(s)	69, 86, 191, 692
BssMI	GATC	4 cut(s)	436, 442, 688, 795
BssNI	GRCGYC	1 cut(s)	126
Bst2UI	CCWGG	4 cut(s)	71, 192, 264, 820
Bst4CI	ACNGT	4 cut(s)	122, 961, 1054, 1275
Bst6I	CTCTTC	2 cut(s)	483, 1311
BstACI	GRCGYC	1 cut(s)	126
BstAFI	CTTAAG	1 cut(s)	974
BstAUI	TGTACA	1 cut(s)	633
BstBAI	YACGTR	1 cut(s)	728
BstBI	TTCGAA	1 cut(s)	1109
BstC8I	GCNNGC	2 cut(s)	1219, 1311
BstDEI	CTNAG	4 cut(s)	180, 226, 915, 1122
BstENI	CCTNNNNNAGG	1 cut(s)	592
BstF5I	GGATG	5 cut(s)	106, 233, 259, 313, 1069
BstHHI	GCGC	1 cut(s)	667
BstKTI	GATC	4 cut(s)	439, 445, 691, 798
BstMAI	GTCTC	1 cut(s)	1030
BstMBI	GATC	4 cut(s)	436, 442, 688, 795
BstNI	CCWGG	4 cut(s)	71, 192, 264, 820
BstNSI	RCATGY	2 cut(s)	994, 1202
BstSCI	CCNGG	5 cut(s)	69, 85, 190, 262, 818
BstSLI	GKGCMC	1 cut(s)	70
BstV1I	GCAGC	3 cut(s)	310, 669, 1273
BsuRI	GGCC	2 cut(s)	586, 614
BtsCI	GGATG	5 cut(s)	106, 233, 259, 313, 1069
BtsI	GCAGTG	2 cut(s)	43, 449
BtsIMutI	CAGTG	6 cut(s)	43, 127, 177, 449, 622, 1280
Cac8I	GCNNGC	2 cut(s)	1219, 1311
CciI	TCATGA	1 cut(s)	1000
CfoI	GCGC	1 cut(s)	667
Cfr10I	RCCGGY	2 cut(s)	301, 375
Cfr13I	GGNCC	3 cut(s)	430, 585, 1183
Csp6I	GTAC	5 cut(s)	255, 334, 634, 703, 738
CspAI	ACCGGT	1 cut(s)	375
CviAII	CATG	5 cut(s)	238, 991, 1001, 1199, 1350
CviQI	GTAC	5 cut(s)	255, 334, 634, 703, 738
DdeI	CTNAG	4 cut(s)	180, 226, 915, 1122
DpnI	GATC	4 cut(s)	438, 444, 690, 797
DpnII	GATC	4 cut(s)	436, 442, 688, 795
DraI	TTTAAA	1 cut(s)	606
DrdI	GACNNNNNNGTC	2 cut(s)	47, 125
DseDI	GACNNNNNNGTC	2 cut(s)	47, 125
Eam1104I	CTCTTC	2 cut(s)	483, 1311
EarI	CTCTTC	2 cut(s)	483, 1311
Ecl136II	GAGCTC	1 cut(s)	950
Eco24I	GRGCYC	1 cut(s)	952
Eco32I	GATATC	1 cut(s)	860
Eco47I	GGWCC	2 cut(s)	430, 1183
Eco53kI	GAGCTC	1 cut(s)	950
Eco57I	CTGAAG	4 cut(s)	186, 853, 963, 1335
Eco72I	CACGTG	1 cut(s)	728
EcoICRI	GAGCTC	1 cut(s)	950
EcoNI	CCTNNNNNAGG	1 cut(s)	592
EcoO109I	RGGNCCY	1 cut(s)	585
EcoRII	CCWGG	4 cut(s)	69, 190, 262, 818
EcoRV	GATATC	1 cut(s)	860
EcoT22I	ATGCAT	1 cut(s)	567
EcoT38I	GRGCYC	1 cut(s)	952
FaeI	CATG	5 cut(s)	241, 994, 1004, 1202, 1353
FalI	AAGNNNNNCTT	4 cut(s)	410, 442, 936, 968
FatI	CATG	5 cut(s)	237, 990, 1000, 1198, 1349
FauNDI	CATATG	2 cut(s)	477, 567
FblI	GTMKAC	2 cut(s)	197, 555
Fnu4HI	GCNGC	3 cut(s)	299, 683, 1287
FokI	GGATG	5 cut(s)	113, 220, 246, 320, 1076
FriOI	GRGCYC	1 cut(s)	952
Fsp4HI	GCNGC	3 cut(s)	299, 683, 1287
FspBI	CTAG	5 cut(s)	33, 318, 440, 678, 761
GlaI	GCGC	1 cut(s)	666
GluI	GCNGC	3 cut(s)	299, 683, 1287
HaeIII	GGCC	2 cut(s)	586, 614
HapII	CCGG	4 cut(s)	86, 98, 302, 376
HhaI	GCGC	1 cut(s)	667
Hin1I	GRCGYC	1 cut(s)	126
Hin1II	CATG	5 cut(s)	241, 994, 1004, 1202, 1353
Hin6I	GCGC	1 cut(s)	665
HinP1I	GCGC	1 cut(s)	665
HincII	GTYRAC	1 cut(s)	198
HindII	GTYRAC	1 cut(s)	198
HindIII	AAGCTT	1 cut(s)	419
HinfI	GANTC	7 cut(s)	14, 74, 187, 472, 515, 981, 1126
HpaII	CCGG	4 cut(s)	86, 98, 302, 376
HphI	GGTGA	3 cut(s)	9, 87, 1204
Hpy166II	GTNNAC	6 cut(s)	198, 556, 884, 938, 985, 1183
Hpy188I	TCNGA	6 cut(s)	205, 229, 471, 551, 693, 916
Hpy188III	TCNNGA	9 cut(s)	59, 130, 440, 628, 761, 965, 1001, 1138, 1209
Hpy8I	GTNNAC	6 cut(s)	198, 556, 884, 938, 985, 1183
Hpy99I	CGWCG	1 cut(s)	131
HpyAV	CCTTC	4 cut(s)	235, 938, 1054, 1116
HpyCH4III	ACNGT	4 cut(s)	122, 961, 1054, 1275
HpyCH4IV	ACGT	2 cut(s)	126, 727
HpyCH4V	TGCA	7 cut(s)	149, 409, 523, 565, 788, 956, 1289
HpyF3I	CTNAG	4 cut(s)	180, 226, 915, 1122
HpySE526I	ACGT	2 cut(s)	126, 727
Hsp92I	GRCGYC	1 cut(s)	126
Hsp92II	CATG	5 cut(s)	241, 994, 1004, 1202, 1353
HspAI	GCGC	1 cut(s)	665
KpnI	GGTACC	1 cut(s)	741
Kzo9I	GATC	4 cut(s)	436, 442, 688, 795
LmnI	GCTCC	2 cut(s)	947, 1160
Lsp1109I	GCAGC	3 cut(s)	310, 669, 1273
LweI	GCATC	3 cut(s)	242, 1108, 1204
MaeI	CTAG	5 cut(s)	33, 318, 440, 678, 761
MaeII	ACGT	2 cut(s)	126, 727
MaeIII	GTNAC	5 cut(s)	38, 122, 378, 926, 1192
MalI	GATC	4 cut(s)	438, 444, 690, 797
MboI	GATC	4 cut(s)	436, 442, 688, 795
MboII	GAAGA	6 cut(s)	116, 230, 500, 859, 1153, 1328
MhlI	GDGCHC	2 cut(s)	70, 952
MluCI	AATT	8 cut(s)	207, 369, 457, 838, 887, 1004, 1083, 1100
MlyI	GAGTC	1 cut(s)	990
Mph1103I	ATGCAT	1 cut(s)	567
MspCI	CTTAAG	1 cut(s)	974
MspI	CCGG	4 cut(s)	86, 98, 302, 376
MspR9I	CCNGG	5 cut(s)	71, 87, 192, 264, 820
MvaI	CCWGG	4 cut(s)	71, 192, 264, 820
NciI	CCSGG	1 cut(s)	87
NdeI	CATATG	2 cut(s)	477, 567
NdeII	GATC	4 cut(s)	436, 442, 688, 795
NlaIII	CATG	5 cut(s)	241, 994, 1004, 1202, 1353
NlaIV	GGNNCC	3 cut(s)	739, 1162, 1185
NmuCI	GTSAC	4 cut(s)	38, 122, 926, 1192
NsiI	ATGCAT	1 cut(s)	567
NspI	RCATGY	2 cut(s)	994, 1202
NspV	TTCGAA	1 cut(s)	1109
PagI	TCATGA	1 cut(s)	1000
PciI	ACATGT	2 cut(s)	990, 1198
PfeI	GAWTC	6 cut(s)	14, 74, 187, 472, 515, 1126
PflFI	GACNNNGTC	1 cut(s)	47
PinAI	ACCGGT	1 cut(s)	375
PkrI	GCNGC	3 cut(s)	300, 684, 1288
PleI	GAGTC	1 cut(s)	989
PmaCI	CACGTG	1 cut(s)	728
PmlI	CACGTG	1 cut(s)	728
PpsI	GAGTC	1 cut(s)	989
Ppu21I	YACGTR	1 cut(s)	728
PscI	ACATGT	2 cut(s)	990, 1198
Psp124BI	GAGCTC	1 cut(s)	952
Psp6I	CCWGG	4 cut(s)	69, 190, 262, 818
PspCI	CACGTG	1 cut(s)	728
PspGI	CCWGG	4 cut(s)	69, 190, 262, 818
PspN4I	GGNNCC	3 cut(s)	739, 1162, 1185
PspPI	GGNCC	3 cut(s)	430, 585, 1183
PsyI	GACNNNGTC	1 cut(s)	47
RsaI	GTAC	5 cut(s)	256, 335, 635, 704, 739
RsaNI	GTAC	5 cut(s)	255, 334, 634, 703, 738
SacI	GAGCTC	1 cut(s)	952
SalI	GTCGAC	1 cut(s)	196
SatI	GCNGC	3 cut(s)	299, 683, 1287
Sau3AI	GATC	4 cut(s)	436, 442, 688, 795
Sau96I	GGNCC	3 cut(s)	430, 585, 1183
ScaI	AGTACT	1 cut(s)	704
SchI	GAGTC	1 cut(s)	990
ScrFI	CCNGG	5 cut(s)	71, 87, 192, 264, 820
SduI	GDGCHC	2 cut(s)	70, 952
SfaNI	GCATC	3 cut(s)	242, 1108, 1204
SfuI	TTCGAA	1 cut(s)	1109
SinI	GGWCC	2 cut(s)	430, 1183
SmlI	CTYRAG	2 cut(s)	59, 974
SmoI	CTYRAG	2 cut(s)	59, 974
Sse9I	AATT	8 cut(s)	207, 369, 457, 838, 887, 1004, 1083, 1100
SsiI	CCGC	1 cut(s)	1241
SspI	AATATT	2 cut(s)	497, 846
SspMI	CTAG	5 cut(s)	33, 318, 440, 678, 761
SstI	GAGCTC	1 cut(s)	952
StyD4I	CCNGG	5 cut(s)	69, 85, 190, 262, 818
TaaI	ACNGT	4 cut(s)	122, 961, 1054, 1275
TaiI	ACGT	2 cut(s)	129, 730
TaqI	TCGA	6 cut(s)	129, 197, 627, 1109, 1116, 1172
TasI	AATT	8 cut(s)	207, 369, 457, 838, 887, 1004, 1083, 1100
TatI	WGTACW	3 cut(s)	254, 633, 702
TfiI	GAWTC	6 cut(s)	14, 74, 187, 472, 515, 1126
TscAI	CASTG	6 cut(s)	43, 127, 184, 456, 622, 1280
TseFI	GTSAC	4 cut(s)	38, 122, 926, 1192
TseI	GCWGC	3 cut(s)	298, 682, 1286
Tsp45I	GTSAC	4 cut(s)	38, 122, 926, 1192
TspDTI	ATGAA	6 cut(s)	117, 254, 258, 381, 989, 1017
TspRI	CASTG	6 cut(s)	43, 127, 184, 456, 622, 1280
Tth111I	GACNNNGTC	1 cut(s)	47
Vha464I	CTTAAG	1 cut(s)	974
VpaK11BI	GGWCC	2 cut(s)	430, 1183
XagI	CCTNNNNNAGG	1 cut(s)	592
XapI	RAATTY	2 cut(s)	207, 1100
XbaI	TCTAGA	2 cut(s)	439, 760
XceI	RCATGY	2 cut(s)	994, 1202
XmiI	GTMKAC	2 cut(s)	197, 555
XspI	CTAG	5 cut(s)	33, 318, 440, 678, 761
ZraI	GACGTC	1 cut(s)	127
ZrmI	AGTACT	1 cut(s)	704
Zsp2I	ATGCAT	1 cut(s)	567

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.