RchiOBHm_Chr6g0276921

RNA polymerase II C-terminal domain phosphatase-like

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
Physical Location & Seq
Forward (+)
39253680 .. 39255505
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24842

Sequence Viewer

Length: 1248 bp
ATGATGAGTGTGGCTACTGTGTCTCCGATTTTACATAATATTGATAACCGTGTTGGGTATCTTAAGAGGGGACTAGGGTTAGATTCTGATTACAGTGACCTAGGAGTGATAGATCAGAGGGCCAAAAGGCTTAAGGTAGAGACTGATGTGCTGGGAGTTTCAGAAGAGGCTAGGTTTGCTTTTGATGAAAAACCCAGGTTTTCTGAAGTGAGATTTGGGTATATACCTAAGGGGTTATTGCTTAGGAGTGATGAAATTGATCGATTACGCAGCGAAAACACTAGGAATCTATTGTTGAACCATAAGAAACTTCATCTGGTTCTTGATCTCGACCACACCCTGCTGAATACCACTTTCCTTGATGAGATGTCACCCGACGAAGAATATCTGAAGACCCAAACCCAGTCTGATCATTCTCTGCAGTATGTTCACGTTGTTGACACTCCCAGTAGGCAATTGATGACCAAGTTGAGGCCTTATATCAGGACGTTTTTGGTGCAAGCCAGTCAAATGTTTGAGCTGTCCATATACACAATGGGTAATCGAGATTATGCGCTAGAAATGGCTAAGCTGCTTGATCCAGGAAATCATATCTTTGGTGGTAGAGTCATATCGCGTGATGATAGCACAGAAACAGATAGAAAGAGTCTAGATGTCCTGCTTGCCCGAGATTCTGCTGTTGTGATCCTTGATGATACGAAAAATGTATGGACAAATGACAACAGGGACAATGTAATAGTGATGCCTAGGTACCATTTCTTTAGATCTAGTTGTCAAAAGTTTCGCCTCAGTAAGGGTATGCCTTATTCTGAGCTGAAGACTGATGAATGTGATCGTTATGGAGGAGCTTATCTTGCAAATGTACTACGACTTCTTGGGTATATCCACACCATATTCTTTAATGAAGTTGAGTTACAGGGGTGGGATCTTATCGACAGAGATGTGAGGCTTGTCTTGAAAATCCTTAAGAAGGAAGTCTTGAAGGGCTGTAAAATTGTTTTCAGCCATGTATTCCCCTCAAATGTCGAAGCTAATACTCATCCTTTGTGGAAGATGGCGGAGCAGCTGGGCGCTACTTGTTCAACACAAGTCGATCCATCAGTCACACATGTAGTTGCAGCAAATGCTCGAACACAGAAATCGTGTTGGGCAGTTAAAGAAAGAAAGTTTTTGGTGAATCCCCAATGGATTCGTTCTGCAAATTTTATGTGGCAAAAGCAACCTGAAGATAATTTTCCCTGTCAGTAG

Protein Analysis

415

Amino Acids

47.91

Weight (kDa)

7.19

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 105 - 265 2e-24 NLI interacting factor-like phosphatase
BRCT PF00533 325 - 398 5.3e-09 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 346 - 396 6.6e-06 twin BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Orthologous Genes (Group: OG0000221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58003 AT5G58003 AT5G58003
fragaria_vesca FvH4_1g01060 FvH4_1g01060 FvH4_1g01060 FvH4_1g01060 FvH4_2g05941 FvH4_7g02340 FvH4_7g02631 FvH4_7g02801 FvH4_7g07841 FvH4_7g08260
malus_domestica MD09G1131300.v1.1
prunus_persica Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1 Prupe.7G261400_v2.0.a1
pyrus_communis pycom09g05350
rosa_chinensis RchiOBHm_Chr1g0324741 RchiOBHm_Chr2g0085821 RchiOBHm_Chr2g0147611 RchiOBHm_Chr4g0392421 RchiOBHm_Chr6g0254001 RchiOBHm_Chr6g0256111 RchiOBHm_Chr6g0256201 RchiOBHm_Chr6g0256301 RchiOBHm_Chr6g0257451 RchiOBHm_Chr6g0267911 RchiOBHm_Chr6g0276921 RchiOBHm_Chr7g0234511 RchiOBHm_Chr7g0234521 RchiOBHm_Chr7g0234531 RchiOBHm_Chr7g0234711 RchiOBHm_Chr7g0234721 RchiOBHm_Chr7g0234801 RchiOBHm_Chr7g0234841 RchiOBHm_Chr7g0234851
rosa_laevigata RLG00000001210 RLG00000001218 RLG00000013348 RLG00000013351 RLG00000013354 RLG00000013969 RLG00000014699 RLG00000014794 RLG00000014949 RLG00000015721 RLG00000030176 RLG00000030180 RLG00000030183
rosa_multiflora Rmu_sc0000434.1_g000006 Rmu_sc0001716.1_g000020 Rmu_sc0001800.1_g000011 Rmu_sc0001913.1_g000023 Rmu_sc0003301.1_g000015 Rmu_sc0003882.1_g000010 Rmu_sc0005080.1_g000013 Rmu_sc0006724.1_g000007 Rmu_sc0006759.1_g000001 Rmu_sc0006859.1_g000001 Rmu_sc0007217.1_g000009 Rmu_sc0010500.1_g000001 Rmu_sc0015353.1_g000005 Rmu_sc0017253.1_g000006 Rmu_sc0021133.1_g000001 Rmu_sc0033202.1_g000001
rosa_roxburghii Rroxscaffold_2G00155020 Rroxscaffold_4G00325270 Rroxscaffold_7G00200340 Rroxscaffold_7G00211310
rosa_rugosa Rorug01G0046400 Rorug01G0463600 Rorug05G0571900 Rorug05G0572300 Rorug06G0034600 Rorug07G0285100 Rorug07G0285400 Rorug07G0285500 Rorug07G0285600
rosa_samantha Rh1AG063200 Rh1AG063800 Rh1DG069200 Rh1DG070400 Rh2AG012600 Rh2BG013300 Rh2DG015000 Rh4AG042600 Rh4BG037500 Rh4CG045400 Rh4CG045600 Rh6AG065400 Rh6AG082000 Rh6AG082100 Rh6AG089900 Rh6AG156900 Rh6AG157000 Rh6AG157200 Rh6AG214900 Rh6AG215000 Rh6AG215200 Rh6AG291000 Rh6BG058300 Rh6BG074200 Rh6BG074300 Rh6BG081300 Rh6BG158100 Rh6BG158400 Rh6BG158700 Rh6BG219800 Rh6CG058400 Rh6CG071100 Rh6CG078000 Rh6CG157400 Rh6CG158100 Rh6CG222400 Rh6DG055200 Rh6DG068500 Rh6DG073700 Rh6DG143300 Rh6DG143700 Rh6DG144100 Rh6DG212600 Rh7AG440200 Rh7AG440300 Rh7BG413100 Rh7CG461400 Rh7CG461500 Rh7CG461900 Rh7CG462000 Rh7DG429900
rosa_wichuraiana Rw1G005350 Rw2G001120 Rw4G003330 Rw6G005760 Rw6G007140 Rw6G007840 Rw6G013630 Rw6G013660 Rw6G018800 Rw6G025060 Rw7G036540 Rw7G036580 Rw7G036600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 750
AccB1I GGYRCC 1 cut(s) 750
AccII CGCG 1 cut(s) 616
AciI CCGC 1 cut(s) 1058
AclWI GGATC 4 cut(s) 572, 679, 933, 1088
AcsI RAATTY 1 cut(s) 1201
AcuI CTGAAG 4 cut(s) 225, 410, 836, 1245
AfaI GTAC 2 cut(s) 752, 864
AfiI CCNNNNNNNGG 3 cut(s) 471, 793, 970
AflII CTTAAG 3 cut(s) 62, 131, 965
AflIII ACRYGT 1 cut(s) 1108
AgsI TTSAA 4 cut(s) 298, 958, 982, 1083
AjnI CCWGG 2 cut(s) 194, 580
AjuI GAANNNNNNNTTGG 2 cut(s) 198, 230
AluBI AGCT 6 cut(s) 520, 571, 814, 848, 1031, 1066
AluI AGCT 6 cut(s) 520, 571, 814, 848, 1031, 1066
Alw26I GTCTC 2 cut(s) 27, 134
AlwI GGATC 4 cut(s) 572, 679, 933, 1088
Ama87I CYCGRG 1 cut(s) 666
AoxI GGCC 2 cut(s) 120, 473
ApeKI GCWGC 4 cut(s) 270, 571, 1063, 1118
ApoI RAATTY 1 cut(s) 1201
Asp718I GGTACC 1 cut(s) 750
AspA2I CCTAGG 2 cut(s) 100, 746
AspLEI GCGC 2 cut(s) 556, 1073
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 363, 1186
AvaI CYCGRG 1 cut(s) 666
AvrII CCTAGG 2 cut(s) 100, 746
AxyI CCTNAGG 1 cut(s) 228
BanI GGYRCC 1 cut(s) 750
BarI GAAGNNNNNNTAC 4 cut(s) 855, 887, 897, 929
BbsI GAAGAC 2 cut(s) 398, 824
BbvI GCAGC 4 cut(s) 282, 558, 1075, 1130
BccI CCATC 2 cut(s) 1048, 1105
BciT130I CCWGG 2 cut(s) 196, 582
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 2 cut(s) 27, 134
BfaI CTAG 8 cut(s) 74, 101, 171, 282, 557, 650, 747, 768
BfmI CTRYAG 1 cut(s) 419
BfoI RGCGCY 1 cut(s) 1074
BfrI CTTAAG 3 cut(s) 62, 131, 965
BglII AGATCT 1 cut(s) 764
BisI GCNGC 4 cut(s) 271, 572, 1064, 1119
BlnI CCTAGG 2 cut(s) 100, 746
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 4 cut(s) 272, 573, 1065, 1120
Bme1390I CCNGG 2 cut(s) 196, 582
BmeT110I CYCGRG 1 cut(s) 666
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 752
BmrFI CCNGG 2 cut(s) 196, 582
BmrI ACTGGG 2 cut(s) 397, 441
BmsI GCATC 1 cut(s) 732
BmuI ACTGGG 2 cut(s) 397, 441
BpiI GAAGAC 2 cut(s) 398, 824
Bpu10I CCTNAGC 1 cut(s) 242
Bpu1102I GCTNAGC 1 cut(s) 567
Bsa29I ATCGAT 1 cut(s) 262
BsaJI CCNNGG 3 cut(s) 100, 194, 746
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 3 cut(s) 471, 793, 970
Bse1I ACTGG 3 cut(s) 403, 447, 504
Bse21I CCTNAGG 1 cut(s) 228
BseBI CCWGG 2 cut(s) 196, 582
BseCI ATCGAT 1 cut(s) 262
BseDI CCNNGG 3 cut(s) 100, 194, 746
BseGI GGATG 1 cut(s) 1039
BseLI CCNNNNNNNGG 3 cut(s) 471, 793, 970
BseMII CTCAG 2 cut(s) 801, 802
BseNI ACTGG 3 cut(s) 403, 447, 504
BseRI GAGGAG 1 cut(s) 858
BseXI GCAGC 4 cut(s) 282, 558, 1075, 1130
BseYI CCCAGC 2 cut(s) 151, 1066
Bsh1236I CGCG 1 cut(s) 616
BshFI GGCC 2 cut(s) 122, 475
BshNI GGYRCC 1 cut(s) 750
BshVI ATCGAT 1 cut(s) 262
BsiHKCI CYCGRG 1 cut(s) 666
BslFI GGGAC 2 cut(s) 84, 740
BslI CCNNNNNNNGG 3 cut(s) 471, 793, 970
BsmAI GTCTC 2 cut(s) 27, 134
BsmFI GGGAC 2 cut(s) 84, 740
BsnI GGCC 2 cut(s) 122, 475
BsoBI CYCGRG 1 cut(s) 666
Bsp1720I GCTNAGC 1 cut(s) 567
BspACI CCGC 1 cut(s) 1058
BspANI GGCC 2 cut(s) 122, 475
BspCNI CTCAG 2 cut(s) 801, 802
BspDI ATCGAT 1 cut(s) 262
BspFNI CGCG 1 cut(s) 616
BspLI GGNNCC 1 cut(s) 752
BspMAI CTGCAG 1 cut(s) 423
BspPI GGATC 4 cut(s) 572, 679, 933, 1088
BspT107I GGYRCC 1 cut(s) 750
BspTI CTTAAG 3 cut(s) 62, 131, 965
BsrI ACTGG 3 cut(s) 403, 447, 504
BssECI CCNNGG 3 cut(s) 100, 194, 746
BssT1I CCWWGG 2 cut(s) 100, 746
Bst2UI CCWGG 2 cut(s) 196, 582
Bst4CI ACNGT 3 cut(s) 19, 50, 95
Bst6I CTCTTC 1 cut(s) 159
BstAFI CTTAAG 3 cut(s) 62, 131, 965
BstAPI GCANNNNNTGC 1 cut(s) 1124
BstC8I GCNNGC 2 cut(s) 501, 663
BstDEI CTNAG 5 cut(s) 228, 242, 567, 788, 810
BstENI CCTNNNNNAGG 2 cut(s) 791, 968
BstF5I GGATG 1 cut(s) 1039
BstFNI CGCG 1 cut(s) 616
BstH2I RGCGCY 1 cut(s) 1074
BstHHI GCGC 2 cut(s) 556, 1073
BstMAI GTCTC 2 cut(s) 27, 134
BstMWI GCNNNNNNNGC 3 cut(s) 176, 854, 1124
BstNI CCWGG 2 cut(s) 196, 582
BstNSI RCATGY 1 cut(s) 1112
BstSCI CCNGG 2 cut(s) 194, 580
BstSFI CTRYAG 1 cut(s) 419
BstUI CGCG 1 cut(s) 616
BstV1I GCAGC 4 cut(s) 282, 558, 1075, 1130
BstV2I GAAGAC 2 cut(s) 398, 824
BstX2I RGATCY 2 cut(s) 764, 925
BstYI RGATCY 2 cut(s) 764, 925
Bsu15I ATCGAT 1 cut(s) 262
Bsu36I CCTNAGG 1 cut(s) 228
BsuRI GGCC 2 cut(s) 122, 475
BsuTUI ATCGAT 1 cut(s) 262
BtsCI GGATG 1 cut(s) 1039
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 2 cut(s) 501, 663
CfoI GCGC 2 cut(s) 556, 1073
Cfr13I GGNCC 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 262
Csp6I GTAC 2 cut(s) 751, 863
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 2 cut(s) 1007, 1109
CviQI GTAC 2 cut(s) 751, 863
DdeI CTNAG 5 cut(s) 228, 242, 567, 788, 810
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 1 cut(s) 1073
Eco130I CCWWGG 2 cut(s) 100, 746
Eco147I AGGCCT 1 cut(s) 475
Eco57I CTGAAG 4 cut(s) 225, 410, 836, 1245
Eco81I CCTNAGG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 666
EcoNI CCTNNNNNAGG 2 cut(s) 791, 968
EcoRII CCWGG 2 cut(s) 194, 580
EcoT14I CCWWGG 2 cut(s) 100, 746
ErhI CCWWGG 2 cut(s) 100, 746
FaeI CATG 2 cut(s) 1010, 1112
FalI AAGNNNNNCTT 2 cut(s) 962, 994
FaqI GGGAC 2 cut(s) 84, 740
FatI CATG 2 cut(s) 1006, 1108
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 4 cut(s) 271, 572, 1064, 1119
FokI GGATG 1 cut(s) 1026
Fsp4HI GCNGC 4 cut(s) 271, 572, 1064, 1119
FspBI CTAG 8 cut(s) 74, 101, 171, 282, 557, 650, 747, 768
GlaI GCGC 2 cut(s) 555, 1072
GluI GCNGC 4 cut(s) 271, 572, 1064, 1119
GsaI CCCAGC 2 cut(s) 155, 1070
HaeII RGCGCY 1 cut(s) 1074
HaeIII GGCC 2 cut(s) 122, 475
HhaI GCGC 2 cut(s) 556, 1073
Hin1II CATG 2 cut(s) 1010, 1112
Hin6I GCGC 2 cut(s) 554, 1071
HinP1I GCGC 2 cut(s) 554, 1071
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 7 cut(s) 83, 286, 606, 646, 671, 1177, 1189
HphI GGTGA 2 cut(s) 363, 1186
Hpy166II GTNNAC 2 cut(s) 430, 439
Hpy188I TCNGA 8 cut(s) 27, 88, 117, 163, 205, 390, 409, 811
Hpy188III TCNNGA 7 cut(s) 323, 329, 484, 545, 650, 955, 979
Hpy8I GTNNAC 2 cut(s) 430, 439
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 2 cut(s) 964, 976
HpyCH4III ACNGT 3 cut(s) 19, 50, 95
HpyCH4IV ACGT 2 cut(s) 432, 488
HpyCH4V TGCA 5 cut(s) 421, 499, 857, 1118, 1199
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 854, 1124
HpyF3I CTNAG 5 cut(s) 228, 242, 567, 788, 810
HpySE526I ACGT 2 cut(s) 432, 488
Hsp92II CATG 2 cut(s) 1010, 1112
HspAI GCGC 2 cut(s) 554, 1071
KpnI GGTACC 1 cut(s) 754
Ksp22I TGATCA 1 cut(s) 409
LmnI GCTCC 2 cut(s) 845, 1060
Lsp1109I GCAGC 4 cut(s) 282, 558, 1075, 1130
LweI GCATC 1 cut(s) 732
MaeI CTAG 8 cut(s) 74, 101, 171, 282, 557, 650, 747, 768
MaeII ACGT 2 cut(s) 432, 488
MaeIII GTNAC 4 cut(s) 95, 369, 912, 1102
MboII GAAGA 6 cut(s) 176, 392, 403, 829, 1063, 1238
MfeI CAATTG 1 cut(s) 455
MflI RGATCY 2 cut(s) 764, 925
MluCI AATT 5 cut(s) 255, 455, 993, 1201, 1231
MlyI GAGTC 2 cut(s) 615, 655
MnlI CCTC 8 cut(s) 60, 111, 160, 465, 797, 836, 939, 1027
MseI TTAA 5 cut(s) 63, 132, 900, 966, 1155
MspA1I CMGCKG 1 cut(s) 1066
MspCI CTTAAG 3 cut(s) 62, 131, 965
MspR9I CCNGG 2 cut(s) 196, 582
MunI CAATTG 1 cut(s) 455
MvaI CCWGG 2 cut(s) 196, 582
MvnI CGCG 1 cut(s) 616
MwoI GCNNNNNNNGC 3 cut(s) 176, 854, 1124
NlaIII CATG 2 cut(s) 1010, 1112
NlaIV GGNNCC 1 cut(s) 752
NmuCI GTSAC 3 cut(s) 95, 369, 1102
NspI RCATGY 1 cut(s) 1112
PceI AGGCCT 1 cut(s) 475
PciI ACATGT 1 cut(s) 1108
PfeI GAWTC 5 cut(s) 83, 286, 671, 1177, 1189
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 4 cut(s) 272, 573, 1065, 1120
PleI GAGTC 2 cut(s) 614, 654
PpsI GAGTC 2 cut(s) 614, 654
PscI ACATGT 1 cut(s) 1108
Psp6I CCWGG 2 cut(s) 194, 580
PspFI CCCAGC 2 cut(s) 151, 1066
PspGI CCWGG 2 cut(s) 194, 580
PspN4I GGNNCC 1 cut(s) 752
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 1 cut(s) 423
PsuI RGATCY 2 cut(s) 764, 925
PvuII CAGCTG 1 cut(s) 1066
RsaI GTAC 2 cut(s) 752, 864
RsaNI GTAC 2 cut(s) 751, 863
SaqAI TTAA 5 cut(s) 63, 132, 900, 966, 1155
SatI GCNGC 4 cut(s) 271, 572, 1064, 1119
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 2 cut(s) 615, 655
ScrFI CCNGG 2 cut(s) 196, 582
SfaNI GCATC 1 cut(s) 732
SfcI CTRYAG 1 cut(s) 419
SmlI CTYRAG 3 cut(s) 62, 131, 965
SmoI CTYRAG 3 cut(s) 62, 131, 965
Sse9I AATT 5 cut(s) 255, 455, 993, 1201, 1231
SseBI AGGCCT 1 cut(s) 475
SsiI CCGC 1 cut(s) 1058
SspI AATATT 1 cut(s) 40
SspMI CTAG 8 cut(s) 74, 101, 171, 282, 557, 650, 747, 768
StuI AGGCCT 1 cut(s) 475
StyD4I CCNGG 2 cut(s) 194, 580
StyI CCWWGG 2 cut(s) 100, 746
TaaI ACNGT 3 cut(s) 19, 50, 95
TaiI ACGT 2 cut(s) 435, 491
TaqI TCGA 7 cut(s) 262, 330, 544, 933, 1026, 1092, 1129
TasI AATT 5 cut(s) 255, 455, 993, 1201, 1231
TatI WGTACW 1 cut(s) 862
TfiI GAWTC 5 cut(s) 83, 286, 671, 1177, 1189
Tru1I TTAA 5 cut(s) 63, 132, 900, 966, 1155
Tru9I TTAA 5 cut(s) 63, 132, 900, 966, 1155
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 3 cut(s) 95, 369, 1102
TseI GCWGC 4 cut(s) 270, 571, 1063, 1118
Tsp45I GTSAC 3 cut(s) 95, 369, 1102
TspDTI ATGAA 5 cut(s) 201, 267, 302, 840, 918
TspRI CASTG 1 cut(s) 100
Vha464I CTTAAG 3 cut(s) 62, 131, 965
XagI CCTNNNNNAGG 2 cut(s) 791, 968
XapI RAATTY 1 cut(s) 1201
XbaI TCTAGA 1 cut(s) 649
XceI RCATGY 1 cut(s) 1112
XcmI CCANNNNNNNNNTGG 1 cut(s) 532
XmaJI CCTAGG 2 cut(s) 100, 746
XspI CTAG 8 cut(s) 74, 101, 171, 282, 557, 650, 747, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.