MD13G1184800.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000716
Physical Location & Seq
Forward (+)
15702363 .. 15703679
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1184800.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGGCTTCCTCCTCATTTCACTGCCTTCTCTTTTGCTCATTACTCTTCCACTTGATCTTCGTCCCGTCACTAGCCAAATCGGCATCTTTCAGACCAAAAGCCCTTGTCCTTCCAGTAACAAAAGACTCCTCCACCCTCCAATACCTCACCACTTTGAGCCAAAGAACCCCTCTTGTCCCCATAAAACTCACAGTACATCTTGGAGGTCCGTTCCTTTGGGTTGAATGTGAAGATTATATCTCATCCACCTACAAGCCCGCCCGTTGCAACTCCGCGCAATGCTCACTTGCAAACTCCAAGTCTTGTACTACAGAATGCTACTCTCCCCCATCGCCAGGCTGCAACAACAACACATGTGGTCTCTTACCGGACAACCCCATCAACCGCACCGCCGGGGGCGGTGACCTTGGTCAAGACATGGTGTCCATCCAATCCACAGATGGGTCCAATCCTGGTAGGCTGGTCTCTGTCCCCAACCTACTCTTCACTTGTGGTTCTGCATTTTTGTTGGAAGGTCTAGCCAGTGGTGTCAAGGGCATGGCTGGCCTCGGCCGGGCTAAAATTGGGCTTCCTTCCCAATTCGCTTCCGCTTTTAGCTTCCGTCGAAAATTCGCAATTTGTTTGAGCTCTTCAACGAGTTCTAAAGGTGTTGTGATCTTCGGGGATGGGCCTTATAATCTGCTTCCCAATATTGACGTGTCCAAGTCACTTATTTTCACCCCACTGATCCTCAACCCTGTCAGCACAGCAGGTGCTTATTTCACAGGCGAGCCTTCTTCAGACTACTTCATCAAGGTGAAGTCAATTAAGATCAACGAAAAGGTTGTTAAATTGAATACTTCACTGCTGGCTATTGACAATGAAGGGTATGGTGGCACGAAGCTCAGCACCGTGAATCCTTACACGGTTTTGGAGAAATCAATCTACAATGCCGTGGTCAATGCATTTGTGAAAGAGGTTGCAAACATCCCAAGAGTGAAAGCAGTAGCGCCTTTCGGAGCATGCTTCAATTCGAAGAACATTGGTAGTACGAGGGTTGGGCCTGCTGTGCCTCAAATTGATCTTGTGTTGCAAAGCGAGAGTGTGTACTGGAGGATATTTGGAGCAAATTCAATGGTGCAGGTAAGCGATGATGTGTTGTGTTTAGGCTTTGTGGATGGAGGTGAAAGGCCAAGGACTTCTATTGTGATTGGTGGGCACCAACTGGAGGATAATTTGGTGCAGATTGATTTGGCTGCTTCTAAGCTTGGGTTTAGTTCCTCGCTCTTGTTCAGACAGACAACTTGTGCAAACTTCAACTTCACTTCTAATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

439

Amino Acids

46.69

Weight (kDa)

8.77

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 48 - 222 1.4e-45 Xylanase inhibitor N-terminal
TAXi_C PF14541 261 - 419 1.2e-61 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AarI CACCTGC 1 cut(s) 740
Acc36I ACCTGC 2 cut(s) 740, 1111
AccB1I GGYRCC 1 cut(s) 1197
AccII CGCG 1 cut(s) 275
AciI CCGC 6 cut(s) 258, 273, 385, 390, 399, 588
AclWI GGATC 1 cut(s) 721
AcoI YGGCCR 1 cut(s) 550
AcsI RAATTY 2 cut(s) 608, 1108
AcuI CTGAAG 1 cut(s) 762
AfaI GTAC 4 cut(s) 195, 307, 1030, 1088
AfiI CCNNNNNNNGG 4 cut(s) 335, 441, 553, 1207
AflIII ACRYGT 2 cut(s) 353, 696
AgsI TTSAA 6 cut(s) 224, 633, 835, 1009, 1113, 1297
AhdI GACNNNNNGTC 1 cut(s) 421
AjiI CACGTC 1 cut(s) 697
AjnI CCWGG 2 cut(s) 334, 451
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AluBI AGCT 4 cut(s) 597, 627, 883, 1246
AluI AGCT 4 cut(s) 597, 627, 883, 1246
Alw21I GWGCWC 1 cut(s) 629
Alw26I GTCTC 2 cut(s) 365, 469
AlwI GGATC 1 cut(s) 721
AoxI GGCC 5 cut(s) 544, 550, 668, 1040, 1169
ApeKI GCWGC 2 cut(s) 339, 1235
ApoI RAATTY 2 cut(s) 608, 1108
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AspLEI GCGC 2 cut(s) 277, 991
AspS9I GGNCC 4 cut(s) 206, 444, 668, 1040
AsuC2I CCSGG 2 cut(s) 394, 554
AsuHPI GGTGA 5 cut(s) 139, 413, 709, 808, 1175
AsuII TTCGAA 1 cut(s) 1013
AvaII GGWCC 2 cut(s) 206, 444
BaeGI GKGCMC 1 cut(s) 1200
BanI GGYRCC 1 cut(s) 1197
BanII GRGCYC 1 cut(s) 629
Bbv12I GWGCWC 1 cut(s) 629
BbvI GCAGC 2 cut(s) 326, 1222
BccI CCATC 6 cut(s) 337, 386, 434, 434, 659, 1151
BceAI ACGGC 1 cut(s) 917
BciT130I CCWGG 2 cut(s) 336, 453
BcnI CCSGG 2 cut(s) 394, 554
BcoDI GTCTC 2 cut(s) 365, 469
BfaI CTAG 2 cut(s) 71, 518
BfmI CTRYAG 1 cut(s) 309
BfoI RGCGCY 1 cut(s) 992
BfuAI ACCTGC 2 cut(s) 740, 1111
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 2 cut(s) 340, 1236
BlpI GCTNAGC 1 cut(s) 884
BlsI GCNGC 2 cut(s) 341, 1237
Bme1390I CCNGG 4 cut(s) 336, 394, 453, 554
Bme18I GGWCC 2 cut(s) 206, 444
BmeRI GACNNNNNGTC 1 cut(s) 421
BmgBI CACGTC 1 cut(s) 697
BmgT120I GGNCC 4 cut(s) 206, 444, 668, 1040
BmiI GGNNCC 2 cut(s) 445, 1199
BmrFI CCNGG 4 cut(s) 336, 394, 453, 554
BmsI GCATC 1 cut(s) 92
BoxI GACNNNNGTC 1 cut(s) 408
BpmI CTGGAG 2 cut(s) 1111, 1226
Bpu1102I GCTNAGC 1 cut(s) 884
Bpu14I TTCGAA 1 cut(s) 1013
BpuMI CCSGG 2 cut(s) 394, 554
BsaI GGTCTC 2 cut(s) 365, 469
BsaJI CCNNGG 5 cut(s) 393, 406, 547, 933, 1172
BsaWI WCCGGW 1 cut(s) 367
Bsc4I CCNNNNNNNGG 4 cut(s) 335, 441, 553, 1207
Bse1I ACTGG 4 cut(s) 113, 522, 1094, 1209
Bse3DI GCAATG 1 cut(s) 284
BseBI CCWGG 2 cut(s) 336, 453
BseDI CCNNGG 5 cut(s) 393, 406, 547, 933, 1172
BseGI GGATG 5 cut(s) 242, 426, 670, 966, 1162
BseLI CCNNNNNNNGG 4 cut(s) 335, 441, 553, 1207
BseMI GCAATG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 898
BseNI ACTGG 4 cut(s) 113, 522, 1094, 1209
BseRI GAGGAG 1 cut(s) 118
BseSI GKGCMC 1 cut(s) 1200
BseX3I CGGCCG 1 cut(s) 550
BseXI GCAGC 2 cut(s) 326, 1222
BsgI GTGCAG 2 cut(s) 1139, 1241
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 5 cut(s) 546, 552, 670, 1042, 1171
BshNI GGYRCC 1 cut(s) 1197
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 1 cut(s) 629
BsiSI CCGG 3 cut(s) 368, 393, 553
BslFI GGGAC 3 cut(s) 47, 161, 455
BslI CCNNNNNNNGG 4 cut(s) 335, 441, 553, 1207
BsmAI GTCTC 2 cut(s) 365, 469
BsmFI GGGAC 3 cut(s) 47, 161, 455
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 5 cut(s) 546, 552, 670, 1042, 1171
Bso31I GGTCTC 2 cut(s) 365, 469
Bsp119I TTCGAA 1 cut(s) 1013
Bsp1286I GDGCHC 2 cut(s) 629, 1200
Bsp143I GATC 5 cut(s) 54, 654, 726, 810, 1060
Bsp1720I GCTNAGC 1 cut(s) 884
BspACI CCGC 6 cut(s) 258, 273, 385, 390, 399, 588
BspANI GGCC 5 cut(s) 546, 552, 670, 1042, 1171
BspCNI CTCAG 1 cut(s) 897
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 2 cut(s) 445, 1199
BspMI ACCTGC 2 cut(s) 740, 1111
BspPI GGATC 1 cut(s) 721
BspQI GCTCTTC 1 cut(s) 634
BspT104I TTCGAA 1 cut(s) 1013
BspT107I GGYRCC 1 cut(s) 1197
BspTNI GGTCTC 2 cut(s) 365, 469
BsrDI GCAATG 1 cut(s) 284
BsrI ACTGG 4 cut(s) 113, 522, 1094, 1209
BssECI CCNNGG 5 cut(s) 393, 406, 547, 933, 1172
BssMI GATC 5 cut(s) 54, 654, 726, 810, 1060
BssT1I CCWWGG 2 cut(s) 406, 1172
Bst2UI CCWGG 2 cut(s) 336, 453
Bst4CI ACNGT 3 cut(s) 193, 892, 907
Bst6I CTCTTC 3 cut(s) 50, 488, 634
BstBI TTCGAA 1 cut(s) 1013
BstC8I GCNNGC 6 cut(s) 258, 544, 770, 849, 1003, 1044
BstDEI CTNAG 2 cut(s) 884, 1242
BstDSI CCRYGG 1 cut(s) 933
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 5 cut(s) 242, 426, 670, 966, 1162
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 1 cut(s) 992
BstHHI GCGC 2 cut(s) 277, 991
BstKTI GATC 5 cut(s) 57, 657, 729, 813, 1063
BstMAI GTCTC 2 cut(s) 365, 469
BstMBI GATC 5 cut(s) 54, 654, 726, 810, 1060
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 3 cut(s) 80, 543, 1048
BstNI CCWGG 2 cut(s) 336, 453
BstNSI RCATGY 2 cut(s) 357, 1005
BstPAI GACNNNNGTC 1 cut(s) 408
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 4 cut(s) 334, 392, 451, 552
BstSFI CTRYAG 1 cut(s) 309
BstSLI GKGCMC 1 cut(s) 1200
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 2 cut(s) 326, 1222
BstZI CGGCCG 1 cut(s) 550
BsuRI GGCC 5 cut(s) 546, 552, 670, 1042, 1171
BtgI CCRYGG 1 cut(s) 933
BtgZI GCGATG 2 cut(s) 315, 1143
BtrI CACGTC 1 cut(s) 697
BtsCI GGATG 5 cut(s) 242, 426, 670, 966, 1162
BtsI GCAGTG 2 cut(s) 19, 842
BtsIMutI CAGTG 4 cut(s) 19, 529, 722, 842
BveI ACCTGC 2 cut(s) 740, 1111
Cac8I GCNNGC 6 cut(s) 258, 544, 770, 849, 1003, 1044
CfoI GCGC 2 cut(s) 277, 991
Cfr13I GGNCC 4 cut(s) 206, 444, 668, 1040
Csp6I GTAC 4 cut(s) 194, 306, 1029, 1087
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviAII CATG 4 cut(s) 354, 418, 538, 1002
CviQI GTAC 4 cut(s) 194, 306, 1029, 1087
DdeI CTNAG 2 cut(s) 884, 1242
DpnI GATC 5 cut(s) 56, 656, 728, 812, 1062
DpnII GATC 5 cut(s) 54, 654, 726, 810, 1060
DriI GACNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 550
EagI CGGCCG 1 cut(s) 550
Eam1104I CTCTTC 3 cut(s) 50, 488, 634
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 3 cut(s) 50, 488, 634
Ecl136II GAGCTC 1 cut(s) 627
EclXI CGGCCG 1 cut(s) 550
Eco130I CCWWGG 2 cut(s) 406, 1172
Eco24I GRGCYC 1 cut(s) 629
Eco31I GGTCTC 2 cut(s) 365, 469
Eco47I GGWCC 2 cut(s) 206, 444
Eco52I CGGCCG 1 cut(s) 550
Eco53kI GAGCTC 1 cut(s) 627
Eco57I CTGAAG 1 cut(s) 762
Eco91I GGTNACC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 627
EcoO65I GGTNACC 1 cut(s) 401
EcoRII CCWGG 2 cut(s) 334, 451
EcoT14I CCWWGG 2 cut(s) 406, 1172
EcoT22I ATGCAT 1 cut(s) 946
EcoT38I GRGCYC 1 cut(s) 629
ErhI CCWWGG 2 cut(s) 406, 1172
FaeI CATG 4 cut(s) 357, 421, 541, 1005
FaiI YATR 8 cut(s) 182, 237, 355, 419, 539, 675, 870, 1003
FaqI GGGAC 3 cut(s) 47, 161, 455
FatI CATG 4 cut(s) 353, 417, 537, 1001
FauI CCCGC 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 340, 1236
FokI GGATG 5 cut(s) 229, 413, 677, 953, 1169
FriOI GRGCYC 1 cut(s) 629
Fsp4HI GCNGC 2 cut(s) 340, 1236
FspBI CTAG 2 cut(s) 71, 518
GlaI GCGC 2 cut(s) 276, 990
GluI GCNGC 2 cut(s) 340, 1236
GsuI CTGGAG 2 cut(s) 1111, 1226
HaeII RGCGCY 1 cut(s) 992
HaeIII GGCC 5 cut(s) 546, 552, 670, 1042, 1171
HapII CCGG 3 cut(s) 368, 393, 553
HhaI GCGC 2 cut(s) 277, 991
Hin1II CATG 4 cut(s) 357, 421, 541, 1005
Hin6I GCGC 2 cut(s) 275, 989
HinP1I GCGC 2 cut(s) 275, 989
HindIII AAGCTT 1 cut(s) 1244
HinfI GANTC 2 cut(s) 125, 895
HpaII CCGG 3 cut(s) 368, 393, 553
HphI GGTGA 5 cut(s) 139, 413, 709, 808, 1175
Hpy166II GTNNAC 1 cut(s) 1087
Hpy188I TCNGA 4 cut(s) 92, 781, 998, 1274
Hpy188III TCNNGA 1 cut(s) 413
Hpy8I GTNNAC 1 cut(s) 1087
Hpy99I CGWCG 1 cut(s) 606
HpyAV CCTTC 6 cut(s) 35, 119, 506, 582, 783, 857
HpyCH4III ACNGT 3 cut(s) 193, 892, 907
HpyCH4IV ACGT 1 cut(s) 696
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 543, 1048
HpyF3I CTNAG 2 cut(s) 884, 1242
HpySE526I ACGT 1 cut(s) 696
Hsp92II CATG 4 cut(s) 357, 421, 541, 1005
HspAI GCGC 2 cut(s) 275, 989
Kzo9I GATC 5 cut(s) 54, 654, 726, 810, 1060
LguI GCTCTTC 1 cut(s) 634
LmnI GCTCC 2 cut(s) 998, 1103
Lsp1109I GCAGC 2 cut(s) 326, 1222
LweI GCATC 1 cut(s) 92
MaeI CTAG 2 cut(s) 71, 518
MaeII ACGT 1 cut(s) 696
MaeIII GTNAC 4 cut(s) 66, 115, 401, 705
MalI GATC 5 cut(s) 56, 656, 728, 812, 1062
MboI GATC 5 cut(s) 54, 654, 726, 810, 1060
MboII GAAGA 8 cut(s) 37, 49, 242, 475, 621, 649, 768, 1027
MhlI GDGCHC 2 cut(s) 629, 1200
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 489
Mph1103I ATGCAT 1 cut(s) 946
MseI TTAA 3 cut(s) 807, 828, 1315
MslI CAYNNNNRTG 1 cut(s) 794
MspI CCGG 3 cut(s) 368, 393, 553
MspR9I CCNGG 4 cut(s) 336, 394, 453, 554
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 2 cut(s) 336, 453
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 80, 543, 1048
NciI CCSGG 2 cut(s) 394, 554
NdeII GATC 5 cut(s) 54, 654, 726, 810, 1060
NlaIII CATG 4 cut(s) 357, 421, 541, 1005
NlaIV GGNNCC 2 cut(s) 445, 1199
NmeAIII GCCGAG 1 cut(s) 528
NmuCI GTSAC 3 cut(s) 66, 401, 705
NsiI ATGCAT 1 cut(s) 946
NspI RCATGY 2 cut(s) 357, 1005
NspV TTCGAA 1 cut(s) 1013
PaeI GCATGC 1 cut(s) 1005
PaqCI CACCTGC 1 cut(s) 740
PciI ACATGT 1 cut(s) 353
PciSI GCTCTTC 1 cut(s) 634
PctI GAATGC 1 cut(s) 320
PfeI GAWTC 1 cut(s) 895
PkrI GCNGC 2 cut(s) 341, 1237
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PscI ACATGT 1 cut(s) 353
PshAI GACNNNNGTC 1 cut(s) 408
PsiI TTATAA 1 cut(s) 675
Psp124BI GAGCTC 1 cut(s) 629
Psp6I CCWGG 2 cut(s) 334, 451
PspEI GGTNACC 1 cut(s) 401
PspGI CCWGG 2 cut(s) 334, 451
PspN4I GGNNCC 2 cut(s) 445, 1199
PspPI GGNCC 4 cut(s) 206, 444, 668, 1040
RsaI GTAC 4 cut(s) 195, 307, 1030, 1088
RsaNI GTAC 4 cut(s) 194, 306, 1029, 1087
RseI CAYNNNNRTG 1 cut(s) 794
SacI GAGCTC 1 cut(s) 629
SapI GCTCTTC 1 cut(s) 634
SaqAI TTAA 3 cut(s) 807, 828, 1315
SatI GCNGC 2 cut(s) 340, 1236
Sau3AI GATC 5 cut(s) 54, 654, 726, 810, 1060
Sau96I GGNCC 4 cut(s) 206, 444, 668, 1040
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 4 cut(s) 336, 394, 453, 554
SduI GDGCHC 2 cut(s) 629, 1200
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 1013
SinI GGWCC 2 cut(s) 206, 444
SmiMI CAYNNNNRTG 1 cut(s) 794
SphI GCATGC 1 cut(s) 1005
SsiI CCGC 6 cut(s) 258, 273, 385, 390, 399, 588
SspI AATATT 1 cut(s) 691
SspMI CTAG 2 cut(s) 71, 518
SstI GAGCTC 1 cut(s) 629
StyD4I CCNGG 4 cut(s) 334, 392, 451, 552
StyI CCWWGG 2 cut(s) 406, 1172
TaaI ACNGT 3 cut(s) 193, 892, 907
TaiI ACGT 1 cut(s) 699
TaqI TCGA 2 cut(s) 604, 1013
TatI WGTACW 3 cut(s) 193, 305, 1086
TfiI GAWTC 1 cut(s) 895
Tru1I TTAA 3 cut(s) 807, 828, 1315
Tru9I TTAA 3 cut(s) 807, 828, 1315
TscAI CASTG 4 cut(s) 26, 529, 729, 849
TseFI GTSAC 3 cut(s) 66, 401, 705
TseI GCWGC 2 cut(s) 339, 1235
Tsp45I GTSAC 3 cut(s) 66, 401, 705
TspDTI ATGAA 2 cut(s) 778, 876
TspGWI ACGGA 2 cut(s) 198, 590
TspRI CASTG 4 cut(s) 26, 529, 729, 849
VpaK11BI GGWCC 2 cut(s) 206, 444
XapI RAATTY 2 cut(s) 608, 1108
XceI RCATGY 2 cut(s) 357, 1005
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XspI CTAG 2 cut(s) 71, 518
Zsp2I ATGCAT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.