Rorug04G0150200

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000716
Physical Location & Seq
Forward (+)
26342631 .. 26343632
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0150200.1

Sequence Viewer

Length: 1002 bp
ATGTCAAGGCCTTCATTGAATCAACGAAAACACAAGTCCAAGCAGTCGTCTAAGCAGAGACGAGAATTTCATGCTCATAATGAAGATAACAAAGTGTTGACAAGAAAAGTCCGGATAATGTTTAACGACCCCGATGCTACTGATTCTTCATCCTCGAGCGAAGATGAAACGATGGAGACTGCACAGTATGAGAAGAAAAAATCATTAAAAAAATCAAAAAGGTTTGTACAGGAAGTCATTCATTCTTTCTTCATGCATTCAGGTAGGGCAGTTGGTTGTGAAGCACCACCAGAGACTAGAACGACTAGAACTAGCTCAGGAAGAAAGCGATTCTCGTCTTCACCTTTCAGAGGTGTAAGGCAAAGAAAATATGGTAAATGGGCTGCTGAGATTCGAGATCCTATCAAAGGTGGCCGACTTTGGTTGGGTACGTACGCCACTGCCGAGGAAGCTTCCCAGGCTTACGAGACCATGAGGTGTAAGTTCGATGAAATTTTGAGGGCCACTACAACCAGTATTTCTGACAAAAGCAACAAAGATGACAAGAGTAATTCTGACCACGAACACCTATTTCTTTTAAGCAAAGCAACTCCAATAACAGCTTCCAACTCTGGTGGCAACGGCCTTTTGTTCCGTAAGGGATCTCCGGTTGAGGTTCTTGATTTGGGAAAGAATGCGGATTCAGACAGGGAAAACATTGGCACTAATAATGTTGGACAACTGCGGATACCATTGGACATGAGTTTCCTGGATGAAGCATTGCAGTCTCTCCCCACTGATCAGCAACAGCTAGACGTCTTCAACTTTGTACCAGATAATGAGGAGCTTGACAAACTTGCCTCCTCCATTGCCTCCTCTTCTTTAGAGGAACTTGGTAAGTATTTTGACGGAATGTATTGTAGCATTGATGATATAAAGATAGGTGGAGTTGATGATGATAACCCCACCAATCTTCCTGACTGTGACGACTTTGACGATATTCTAATAGTTCAATCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

333

Amino Acids

37.41

Weight (kDa)

5.89

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 116 - 163 1.1e-09 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 798
AccIII TCCGGA 1 cut(s) 111
AciI CCGC 2 cut(s) 677, 724
AclWI GGATC 2 cut(s) 392, 649
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 65, 492
AcyI GRCGYC 1 cut(s) 795
AfaI GTAC 4 cut(s) 228, 430, 434, 810
AfiI CCNNNNNNNGG 2 cut(s) 350, 407
AgsI TTSAA 3 cut(s) 19, 802, 992
AjnI CCWGG 2 cut(s) 456, 747
AjuI GAANNNNNNNTTGG 2 cut(s) 586, 618
AluBI AGCT 5 cut(s) 315, 452, 602, 790, 826
AluI AGCT 5 cut(s) 315, 452, 602, 790, 826
Alw26I GTCTC 5 cut(s) 52, 170, 287, 461, 771
AlwI GGATC 2 cut(s) 392, 649
Ama87I CYCGRG 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 111
AoxI GGCC 4 cut(s) 8, 412, 501, 622
ApeKI GCWGC 1 cut(s) 383
ApoI RAATTY 2 cut(s) 65, 492
Asp700I GAANNNNTTC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 501
AsuHPI GGTGA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 154
BbsI GAAGAC 2 cut(s) 330, 790
BbvI GCAGC 1 cut(s) 370
BccI CCATC 1 cut(s) 166
BceAI ACGGC 1 cut(s) 637
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 2 cut(s) 458, 749
BciVI GTATCC 1 cut(s) 720
BclI TGATCA 1 cut(s) 778
BcoDI GTCTC 5 cut(s) 52, 170, 287, 461, 771
BfaI CTAG 4 cut(s) 297, 306, 312, 791
BfuI GTATCC 1 cut(s) 720
BisI GCNGC 1 cut(s) 384
BlsI GCNGC 1 cut(s) 385
Bme1390I CCNGG 2 cut(s) 458, 749
BmeT110I CYCGRG 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 501
BmrFI CCNGG 2 cut(s) 458, 749
BmsI GCATC 1 cut(s) 124
BpiI GAAGAC 2 cut(s) 330, 790
Bpu10I CCTNAGC 1 cut(s) 316
BsaAI YACGTR 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 795
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 2 cut(s) 444, 456
BsaWI WCCGGW 2 cut(s) 111, 646
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 407
Bse1I ACTGG 1 cut(s) 513
Bse3DI GCAATG 2 cut(s) 758, 846
BseAI TCCGGA 1 cut(s) 111
BseBI CCWGG 2 cut(s) 458, 749
BseDI CCNNGG 2 cut(s) 444, 456
BseGI GGATG 2 cut(s) 149, 757
BseLI CCNNNNNNNGG 2 cut(s) 350, 407
BseMI GCAATG 2 cut(s) 758, 846
BseMII CTCAG 2 cut(s) 330, 378
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 3 cut(s) 832, 836, 844
BseXI GCAGC 1 cut(s) 370
BsgI GTGCAG 1 cut(s) 165
BshFI GGCC 4 cut(s) 10, 414, 503, 624
BsiHKCI CYCGRG 1 cut(s) 154
BsiSI CCGG 2 cut(s) 112, 647
BsiWI CGTACG 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 350, 407
BsmAI GTCTC 5 cut(s) 52, 170, 287, 461, 771
BsmBI CGTCTC 1 cut(s) 52
BsmI GAATGC 2 cut(s) 256, 679
BsnI GGCC 4 cut(s) 10, 414, 503, 624
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 154
Bsp13I TCCGGA 1 cut(s) 111
Bsp1407I TGTACA 1 cut(s) 226
Bsp143I GATC 3 cut(s) 397, 641, 778
BspACI CCGC 2 cut(s) 677, 724
BspANI GGCC 4 cut(s) 10, 414, 503, 624
BspCNI CTCAG 2 cut(s) 329, 379
BspEI TCCGGA 1 cut(s) 111
BspPI GGATC 2 cut(s) 392, 649
BspTNI GGTCTC 1 cut(s) 461
BsrDI GCAATG 2 cut(s) 758, 846
BsrGI TGTACA 1 cut(s) 226
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 2 cut(s) 444, 456
BssMI GATC 3 cut(s) 397, 641, 778
BssNI GRCGYC 1 cut(s) 795
Bst2UI CCWGG 2 cut(s) 458, 749
Bst4CI ACNGT 2 cut(s) 186, 962
Bst6I CTCTTC 1 cut(s) 862
BstACI GRCGYC 1 cut(s) 795
BstAUI TGTACA 1 cut(s) 226
BstBAI YACGTR 1 cut(s) 432
BstDEI CTNAG 3 cut(s) 51, 316, 387
BstENI CCTNNNNNAGG 2 cut(s) 348, 405
BstF5I GGATG 2 cut(s) 149, 757
BstKTI GATC 3 cut(s) 400, 644, 781
BstMAI GTCTC 5 cut(s) 52, 170, 287, 461, 771
BstMBI GATC 3 cut(s) 397, 641, 778
BstMWI GCNNNNNNNGC 2 cut(s) 449, 458
BstNI CCWGG 2 cut(s) 458, 749
BstSCI CCNGG 2 cut(s) 456, 747
BstSNI TACGTA 1 cut(s) 432
BstV1I GCAGC 1 cut(s) 370
BstV2I GAAGAC 2 cut(s) 330, 790
BstX2I RGATCY 2 cut(s) 397, 641
BstYI RGATCY 2 cut(s) 397, 641
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 4 cut(s) 10, 414, 503, 624
BtsCI GGATG 2 cut(s) 149, 757
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 2 cut(s) 438, 774
Cfr13I GGNCC 1 cut(s) 501
Csp6I GTAC 4 cut(s) 227, 429, 433, 809
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviAII CATG 4 cut(s) 71, 253, 472, 739
CviQI GTAC 4 cut(s) 227, 429, 433, 809
DdeI CTNAG 3 cut(s) 51, 316, 387
DpnI GATC 3 cut(s) 399, 643, 780
DpnII GATC 3 cut(s) 397, 641, 778
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 1 cut(s) 862
EarI CTCTTC 1 cut(s) 862
Eco105I TACGTA 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 10
Eco31I GGTCTC 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 154
EcoNI CCTNNNNNAGG 2 cut(s) 348, 405
EcoRII CCWGG 2 cut(s) 456, 747
EcoT22I ATGCAT 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 4 cut(s) 74, 256, 475, 742
FaiI YATR 8 cut(s) 72, 78, 189, 254, 372, 473, 740, 914
FatI CATG 4 cut(s) 70, 252, 471, 738
FbaI TGATCA 1 cut(s) 778
Fnu4HI GCNGC 1 cut(s) 384
FokI GGATG 2 cut(s) 136, 764
Fsp4HI GCNGC 1 cut(s) 384
FspBI CTAG 4 cut(s) 297, 306, 312, 791
GluI GCNGC 1 cut(s) 384
HaeIII GGCC 4 cut(s) 10, 414, 503, 624
HapII CCGG 2 cut(s) 112, 647
Hin1I GRCGYC 1 cut(s) 795
Hin1II CATG 4 cut(s) 74, 256, 475, 742
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 5 cut(s) 19, 143, 330, 391, 680
HpaII CCGG 2 cut(s) 112, 647
HphI GGTGA 1 cut(s) 333
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 4 cut(s) 350, 523, 556, 685
Hpy188III TCNNGA 5 cut(s) 112, 318, 395, 659, 956
Hpy8I GTNNAC 1 cut(s) 99
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 2 cut(s) 186, 962
HpyCH4IV ACGT 2 cut(s) 431, 795
HpyCH4V TGCA 3 cut(s) 182, 256, 763
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 458
HpyF3I CTNAG 3 cut(s) 51, 316, 387
HpySE526I ACGT 2 cut(s) 431, 795
Hsp92I GRCGYC 1 cut(s) 795
Hsp92II CATG 4 cut(s) 74, 256, 475, 742
Kpn2I TCCGGA 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 3 cut(s) 397, 641, 778
LmnI GCTCC 1 cut(s) 823
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 1 cut(s) 124
MaeI CTAG 4 cut(s) 297, 306, 312, 791
MaeII ACGT 2 cut(s) 431, 795
MaeIII GTNAC 1 cut(s) 962
MalI GATC 3 cut(s) 399, 643, 780
MboI GATC 3 cut(s) 397, 641, 778
MflI RGATCY 2 cut(s) 397, 641
MluCI AATT 3 cut(s) 65, 492, 550
MmeI TCCRAC 2 cut(s) 630, 694
Mph1103I ATGCAT 1 cut(s) 258
MroI TCCGGA 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 3 cut(s) 123, 206, 578
MspI CCGG 2 cut(s) 112, 647
MspR9I CCNGG 2 cut(s) 458, 749
Mva1269I GAATGC 2 cut(s) 256, 679
MvaI CCWGG 2 cut(s) 458, 749
MwoI GCNNNNNNNGC 2 cut(s) 449, 458
NdeII GATC 3 cut(s) 397, 641, 778
NlaIII CATG 4 cut(s) 74, 256, 475, 742
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 962
NsiI ATGCAT 1 cut(s) 258
PaeR7I CTCGAG 1 cut(s) 154
PceI AGGCCT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 2 cut(s) 441, 972
PctI GAATGC 2 cut(s) 256, 679
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 5 cut(s) 19, 143, 330, 391, 680
Pfl23II CGTACG 1 cut(s) 432
PfoI TCCNGGA 1 cut(s) 747
PkrI GCNGC 1 cut(s) 385
Ppu21I YACGTR 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 456, 747
PspGI CCWGG 2 cut(s) 456, 747
PspLI CGTACG 1 cut(s) 432
PspPI GGNCC 1 cut(s) 501
PspXI VCTCGAGB 1 cut(s) 154
PsuI RGATCY 2 cut(s) 397, 641
RsaI GTAC 4 cut(s) 228, 430, 434, 810
RsaNI GTAC 4 cut(s) 227, 429, 433, 809
SaqAI TTAA 3 cut(s) 123, 206, 578
SatI GCNGC 1 cut(s) 384
Sau3AI GATC 3 cut(s) 397, 641, 778
Sau96I GGNCC 1 cut(s) 501
ScrFI CCNGG 2 cut(s) 458, 749
SfaNI GCATC 1 cut(s) 124
Sfr274I CTCGAG 1 cut(s) 154
SlaI CTCGAG 1 cut(s) 154
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
SnaBI TACGTA 1 cut(s) 432
Sse9I AATT 3 cut(s) 65, 492, 550
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 2 cut(s) 677, 724
SspMI CTAG 4 cut(s) 297, 306, 312, 791
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 456, 747
TaaI ACNGT 2 cut(s) 186, 962
TaiI ACGT 2 cut(s) 434, 798
TaqI TCGA 3 cut(s) 155, 394, 486
TasI AATT 3 cut(s) 65, 492, 550
TatI WGTACW 1 cut(s) 226
TfiI GAWTC 5 cut(s) 19, 143, 330, 391, 680
Tru1I TTAA 3 cut(s) 123, 206, 578
Tru9I TTAA 3 cut(s) 123, 206, 578
TscAI CASTG 2 cut(s) 445, 781
TseFI GTSAC 1 cut(s) 962
TseI GCWGC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 962
TspDTI ATGAA 8 cut(s) 59, 96, 138, 180, 230, 241, 504, 768
TspGWI ACGGA 2 cut(s) 623, 903
TspRI CASTG 2 cut(s) 445, 781
XagI CCTNNNNNAGG 2 cut(s) 348, 405
XapI RAATTY 2 cut(s) 65, 492
XhoI CTCGAG 1 cut(s) 154
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 4 cut(s) 297, 306, 312, 791
ZraI GACGTC 1 cut(s) 796
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.