Prupe.1G152200_v2.0.a1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000716
Physical Location & Seq
Forward (+)
12016805 .. 12018513
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G152200.1

Sequence Viewer

Length: 1374 bp
ATGGCTTCCTCATCTATTCATAGCCTACTTCTCTTTTGCTCCCTTCTACTCTCGATCCACATGATCCTCTCATCACAAGCTAAACAATCATCACTCAAACCCAAAGCCCTAGTTCTTCCCATAGCCAAAGACGCTTCTACCCTCCAATACATCACCACCATCAAGCAAAGAACCCCACCGGTAACCCTAAAGCTAACCATTGACCTCGGCGGTCAATTCCTCTGGGTTGACTGCGACGAAGGCCAGTACATCTCGTCCACCTACAAGCCTGCCTACTGCAACGCAACACAATGCTCCATTGCAAACTCCAAAGAATGCAACTGGGAATGCTTCTTCCCCCAAAGGCCAGGCTGCAACAACCGCACCTGCATCCTCTTCCCAGACAACACCGTGATTGGCTCTGCCGACAACAATGGAGAGGTTGGTCAGGACATCCTGTGGGGCCTACATTCCACAGATGGGTCCCACCCCGGCCCAAAGGTCTCTAACATTCCAAACTTCATCTTCGCTTGTAGTAGCAATACATTTTACGGTGTCCTTGGCCTTGCCAATGGTGTCAAGGGCATGGCTGGTCTAGGCAGAACCAGAATCGCTCTCCCAACCCAGTTTGCCTCCGCTTATAAACTCCCTAGAAAATTCGCCATTTGCTTGCCTTCTTCGGCTAGATCATACGGTGTTGTTTTCTTCGGGGATGGGCCGTATGTTCTGACTCCCAAGATTGATGTGTATAAGTCGTTGACTTTCACCAAGTTGATCCTCAACCCCGTCAGCACAGGCAATGCTTTTGATCCGGATGAGCCTTCTGCCGAATATTTCATAAATGTGAAGTCAATCAAAGTCAACGAAAAAATCGTGTCACTAAATACTTCTTTGCTAGCGATTGATCAAAATGGTTATGGTGGCACAAAAATCAGCACCGTCAATCCTTACACCGTCTTGGAGAGCACAATCTACAAAGCTTTTGTTGATGCGTTTGTGAAAGAAATGCCTGGTCATAGTAAGAGAGTGGCTTCTGTAGAGCCTTTTGGAGCTTGCTTTGACTCCACCCACATTGGAATTACACGTGTGGGCCCGGCTGTTCCTAGCATTGACTTTGTGTTGCAAAGTGAGGGTGTGTACTGGAGGGTGTCTGGAGGAAATTCCATGGTGCAAGTGAGCAAGGATGTGTTGTGTTTGGGGTTTGTGGGTGCTGAGCAAAGGCAACGCTACTCATTTGAGAGCCCAACGACGTCGATTGTGATCGGAGGGCATCAGTTGGAGGACAATCTTCTTCAGTTTGATCTGGCTAACAAGAGGCTTGGGTTCAGCTCATCACTTTTGTCTCGGGAGACAAGTTGTGCTAACTTCAACTTCACATCTACTGCTGTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

458

Amino Acids

49.59

Weight (kDa)

8.33

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AarI CACCTGC 1 cut(s) 374
AatII GACGTC 1 cut(s) 1232
Acc36I ACCTGC 1 cut(s) 374
AccIII TCCGGA 1 cut(s) 790
AciI CCGC 3 cut(s) 210, 361, 615
AclWI GGATC 4 cut(s) 49, 58, 748, 782
AcsI RAATTY 2 cut(s) 635, 1138
AcuI CTGAAG 1 cut(s) 1256
AcvI CACGTG 1 cut(s) 1064
AcyI GRCGYC 1 cut(s) 1229
AfaI GTAC 2 cut(s) 248, 1118
AfiI CCNNNNNNNGG 1 cut(s) 459
AflIII ACRYGT 2 cut(s) 1061, 1063
AgeI ACCGGT 1 cut(s) 178
AgsI TTSAA 1 cut(s) 1348
AjnI CCWGG 2 cut(s) 346, 988
AluBI AGCT 5 cut(s) 80, 193, 959, 1031, 1308
AluI AGCT 5 cut(s) 80, 193, 959, 1031, 1308
Alw21I GWGCWC 1 cut(s) 947
Alw26I GTCTC 3 cut(s) 487, 1322, 1326
AlwI GGATC 4 cut(s) 49, 58, 748, 782
Ama87I CYCGRG 1 cut(s) 1323
Aor13HI TCCGGA 1 cut(s) 790
AoxI GGCC 7 cut(s) 241, 344, 442, 472, 541, 695, 1069
ApaI GGGCCC 1 cut(s) 1073
ApeKI GCWGC 1 cut(s) 351
ApoI RAATTY 2 cut(s) 635, 1138
AsiGI ACCGGT 1 cut(s) 178
AspS9I GGNCC 6 cut(s) 442, 462, 473, 695, 1069, 1070
AsuC2I CCSGG 2 cut(s) 471, 1073
AsuHPI GGTGA 2 cut(s) 145, 736
AsuNHI GCTAGC 1 cut(s) 874
AvaI CYCGRG 1 cut(s) 1323
AvaII GGWCC 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 1073
BanII GRGCYC 2 cut(s) 1073, 1223
BbrPI CACGTG 1 cut(s) 1064
Bbv12I GWGCWC 1 cut(s) 947
BbvI GCAGC 1 cut(s) 338
BccI CCATC 3 cut(s) 167, 452, 686
BceAI ACGGC 1 cut(s) 682
BciT130I CCWGG 2 cut(s) 348, 990
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 2 cut(s) 471, 1073
BcoDI GTCTC 3 cut(s) 487, 1322, 1326
BfaI CTAG 6 cut(s) 110, 575, 630, 663, 875, 1083
BfmI CTRYAG 1 cut(s) 1014
BfuAI ACCTGC 1 cut(s) 374
BisI GCNGC 1 cut(s) 352
BlpI GCTNAGC 1 cut(s) 1191
BlsI GCNGC 1 cut(s) 353
Bme1390I CCNGG 4 cut(s) 348, 471, 990, 1073
Bme18I GGWCC 1 cut(s) 462
BmeT110I CYCGRG 1 cut(s) 1323
BmgT120I GGNCC 6 cut(s) 442, 462, 473, 695, 1069, 1070
BmiI GGNNCC 4 cut(s) 443, 463, 464, 1071
BmrFI CCNGG 4 cut(s) 348, 471, 990, 1073
BmrI ACTGGG 2 cut(s) 331, 598
BmsI GCATC 3 cut(s) 378, 958, 1258
BmtI GCTAGC 1 cut(s) 878
BmuI ACTGGG 2 cut(s) 331, 598
BpmI CTGGAG 2 cut(s) 1141, 1152
Bpu1102I GCTNAGC 1 cut(s) 1191
BpuMI CCSGG 2 cut(s) 471, 1073
BsaAI YACGTR 1 cut(s) 1064
BsaBI GATNNNNATC 1 cut(s) 1238
BsaHI GRCGYC 1 cut(s) 1229
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 4 cut(s) 205, 469, 538, 1143
BsaWI WCCGGW 2 cut(s) 178, 790
Bsc4I CCNNNNNNNGG 1 cut(s) 459
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 4 cut(s) 244, 326, 604, 1124
Bse3DI GCAATG 2 cut(s) 297, 784
Bse8I GATNNNNATC 1 cut(s) 1238
BseAI TCCGGA 1 cut(s) 790
BseBI CCWGG 2 cut(s) 348, 990
BseDI CCNNGG 4 cut(s) 205, 469, 538, 1143
BseGI GGATG 5 cut(s) 369, 432, 697, 799, 1168
BseJI GATNNNNATC 1 cut(s) 1238
BseLI CCNNNNNNNGG 1 cut(s) 459
BseMI GCAATG 2 cut(s) 297, 784
BseMII CTCAG 1 cut(s) 1182
BseNI ACTGG 4 cut(s) 244, 326, 604, 1124
BseSI GKGCMC 1 cut(s) 1073
BseXI GCAGC 1 cut(s) 338
BshFI GGCC 7 cut(s) 243, 346, 444, 474, 543, 697, 1071
BshTI ACCGGT 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 947
BsiHKCI CYCGRG 1 cut(s) 1323
BsiSI CCGG 4 cut(s) 179, 471, 791, 1073
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 459
BsmAI GTCTC 3 cut(s) 487, 1322, 1326
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 2 cut(s) 320, 332
BsnI GGCC 7 cut(s) 243, 346, 444, 474, 543, 697, 1071
Bso31I GGTCTC 1 cut(s) 487
BsoBI CYCGRG 1 cut(s) 1323
Bsp120I GGGCCC 1 cut(s) 1069
Bsp1286I GDGCHC 3 cut(s) 947, 1073, 1223
Bsp13I TCCGGA 1 cut(s) 790
Bsp143I GATC 8 cut(s) 54, 63, 665, 753, 787, 883, 1239, 1279
Bsp1720I GCTNAGC 1 cut(s) 1191
Bsp19I CCATGG 1 cut(s) 1143
BspACI CCGC 3 cut(s) 210, 361, 615
BspANI GGCC 7 cut(s) 243, 346, 444, 474, 543, 697, 1071
BspCNI CTCAG 1 cut(s) 1183
BspEI TCCGGA 1 cut(s) 790
BspLI GGNNCC 4 cut(s) 443, 463, 464, 1071
BspMI ACCTGC 1 cut(s) 374
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 4 cut(s) 49, 58, 748, 782
BspTNI GGTCTC 1 cut(s) 487
BsrDI GCAATG 2 cut(s) 297, 784
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 4 cut(s) 244, 326, 604, 1124
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 4 cut(s) 205, 469, 538, 1143
BssMI GATC 8 cut(s) 54, 63, 665, 753, 787, 883, 1239, 1279
BssNI GRCGYC 1 cut(s) 1229
BssT1I CCWWGG 2 cut(s) 538, 1143
Bst2UI CCWGG 2 cut(s) 348, 990
Bst4CI ACNGT 5 cut(s) 391, 533, 674, 919, 934
Bst6I CTCTTC 1 cut(s) 380
BstACI GRCGYC 1 cut(s) 1229
BstBAI YACGTR 1 cut(s) 1064
BstC8I GCNNGC 4 cut(s) 270, 650, 876, 1033
BstDEI CTNAG 1 cut(s) 1191
BstDSI CCRYGG 1 cut(s) 1143
BstEII GGTNACC 1 cut(s) 181
BstF5I GGATG 5 cut(s) 369, 432, 697, 799, 1168
BstKTI GATC 8 cut(s) 57, 66, 668, 756, 790, 886, 1242, 1282
BstMAI GTCTC 3 cut(s) 487, 1322, 1326
BstMBI GATC 8 cut(s) 54, 63, 665, 753, 787, 883, 1239, 1279
BstMWI GCNNNNNNNGC 3 cut(s) 131, 240, 360
BstNI CCWGG 2 cut(s) 348, 990
BstPI GGTNACC 1 cut(s) 181
BstSCI CCNGG 4 cut(s) 346, 469, 988, 1071
BstSFI CTRYAG 1 cut(s) 1014
BstSLI GKGCMC 1 cut(s) 1073
BstV1I GCAGC 1 cut(s) 338
BsuRI GGCC 7 cut(s) 243, 346, 444, 474, 543, 697, 1071
BtgI CCRYGG 1 cut(s) 1143
BtsCI GGATG 5 cut(s) 369, 432, 697, 799, 1168
BveI ACCTGC 1 cut(s) 374
Cac8I GCNNGC 4 cut(s) 270, 650, 876, 1033
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 6 cut(s) 442, 462, 473, 695, 1069, 1070
CseI GACGC 1 cut(s) 140
Csp6I GTAC 2 cut(s) 247, 1117
CspAI ACCGGT 1 cut(s) 178
CspCI CAANNNNNGTGG 2 cut(s) 1033, 1068
CviAII CATG 3 cut(s) 61, 565, 1144
CviQI GTAC 2 cut(s) 247, 1117
DdeI CTNAG 1 cut(s) 1191
DpnI GATC 8 cut(s) 56, 65, 667, 755, 789, 885, 1241, 1281
DpnII GATC 8 cut(s) 54, 63, 665, 753, 787, 883, 1239, 1279
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 538, 1143
Eco24I GRGCYC 2 cut(s) 1073, 1223
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 462
Eco57I CTGAAG 1 cut(s) 1256
Eco72I CACGTG 1 cut(s) 1064
Eco88I CYCGRG 1 cut(s) 1323
Eco91I GGTNACC 1 cut(s) 181
EcoO109I RGGNCCY 2 cut(s) 442, 462
EcoO65I GGTNACC 1 cut(s) 181
EcoRII CCWGG 2 cut(s) 346, 988
EcoT14I CCWWGG 2 cut(s) 538, 1143
EcoT38I GRGCYC 2 cut(s) 1073, 1223
ErhI CCWWGG 2 cut(s) 538, 1143
FaeI CATG 3 cut(s) 64, 568, 1147
FaqI GGGAC 1 cut(s) 448
FatI CATG 3 cut(s) 60, 564, 1143
FbaI TGATCA 1 cut(s) 883
Fnu4HI GCNGC 1 cut(s) 352
FokI GGATG 5 cut(s) 356, 419, 704, 806, 1175
FriOI GRGCYC 2 cut(s) 1073, 1223
Fsp4HI GCNGC 1 cut(s) 352
FspBI CTAG 6 cut(s) 110, 575, 630, 663, 875, 1083
GluI GCNGC 1 cut(s) 352
GsuI CTGGAG 2 cut(s) 1141, 1152
HaeIII GGCC 7 cut(s) 243, 346, 444, 474, 543, 697, 1071
HapII CCGG 4 cut(s) 179, 471, 791, 1073
HgaI GACGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 1229
Hin1II CATG 3 cut(s) 64, 568, 1147
HincII GTYRAC 3 cut(s) 229, 738, 841
HindII GTYRAC 3 cut(s) 229, 738, 841
HindIII AAGCTT 1 cut(s) 957
HinfI GANTC 3 cut(s) 588, 709, 1040
HpaII CCGG 4 cut(s) 179, 471, 791, 1073
HphI GGTGA 2 cut(s) 145, 736
Hpy166II GTNNAC 5 cut(s) 229, 258, 738, 841, 1117
Hpy188I TCNGA 2 cut(s) 708, 1244
Hpy188III TCNNGA 5 cut(s) 52, 428, 791, 1131, 1325
Hpy8I GTNNAC 5 cut(s) 229, 258, 738, 841, 1117
Hpy99I CGWCG 3 cut(s) 239, 1231, 1234
HpyAV CCTTC 4 cut(s) 53, 233, 663, 810
HpyCH4III ACNGT 5 cut(s) 391, 533, 674, 919, 934
HpyCH4IV ACGT 2 cut(s) 1063, 1229
HpyCH4V TGCA 7 cut(s) 279, 302, 318, 354, 369, 1102, 1150
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 240, 360
HpyF3I CTNAG 1 cut(s) 1191
HpySE526I ACGT 2 cut(s) 1063, 1229
Hsp92I GRCGYC 1 cut(s) 1229
Hsp92II CATG 3 cut(s) 64, 568, 1147
KflI GGGWCCC 1 cut(s) 462
Kpn2I TCCGGA 1 cut(s) 790
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 8 cut(s) 54, 63, 665, 753, 787, 883, 1239, 1279
LmnI GCTCC 3 cut(s) 44, 299, 1028
Lsp1109I GCAGC 1 cut(s) 338
LweI GCATC 3 cut(s) 378, 958, 1258
MaeI CTAG 6 cut(s) 110, 575, 630, 663, 875, 1083
MaeII ACGT 2 cut(s) 1063, 1229
MaeIII GTNAC 2 cut(s) 181, 855
MalI GATC 8 cut(s) 56, 65, 667, 755, 789, 885, 1241, 1281
MboI GATC 8 cut(s) 54, 63, 665, 753, 787, 883, 1239, 1279
MboII GAAGA 8 cut(s) 107, 325, 367, 496, 648, 676, 1259, 1262
MhlI GDGCHC 3 cut(s) 947, 1073, 1223
MluCI AATT 4 cut(s) 215, 635, 1056, 1138
MlyI GAGTC 2 cut(s) 703, 1034
MmeI TCCRAC 1 cut(s) 1236
MroI TCCGGA 1 cut(s) 790
MseI TTAA 1 cut(s) 1372
MslI CAYNNNNRTG 1 cut(s) 821
MspI CCGG 4 cut(s) 179, 471, 791, 1073
MspR9I CCNGG 4 cut(s) 348, 471, 990, 1073
Mva1269I GAATGC 2 cut(s) 320, 332
MvaI CCWGG 2 cut(s) 348, 990
MwoI GCNNNNNNNGC 3 cut(s) 131, 240, 360
NciI CCSGG 2 cut(s) 471, 1073
NcoI CCATGG 1 cut(s) 1143
NdeII GATC 8 cut(s) 54, 63, 665, 753, 787, 883, 1239, 1279
NheI GCTAGC 1 cut(s) 874
NlaIII CATG 3 cut(s) 64, 568, 1147
NlaIV GGNNCC 4 cut(s) 443, 463, 464, 1071
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 855
PaqCI CACCTGC 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 849
PctI GAATGC 2 cut(s) 320, 332
PfeI GAWTC 1 cut(s) 588
PinAI ACCGGT 1 cut(s) 178
PkrI GCNGC 1 cut(s) 353
PleI GAGTC 2 cut(s) 703, 1034
PmaCI CACGTG 1 cut(s) 1064
PmlI CACGTG 1 cut(s) 1064
PpsI GAGTC 2 cut(s) 703, 1034
Ppu21I YACGTR 1 cut(s) 1064
PpuMI RGGWCCY 1 cut(s) 462
PsiI TTATAA 1 cut(s) 621
Psp5II RGGWCCY 1 cut(s) 462
Psp6I CCWGG 2 cut(s) 346, 988
PspCI CACGTG 1 cut(s) 1064
PspEI GGTNACC 1 cut(s) 181
PspGI CCWGG 2 cut(s) 346, 988
PspN4I GGNNCC 4 cut(s) 443, 463, 464, 1071
PspOMI GGGCCC 1 cut(s) 1069
PspPI GGNCC 6 cut(s) 442, 462, 473, 695, 1069, 1070
PspPPI RGGWCCY 1 cut(s) 462
RsaI GTAC 2 cut(s) 248, 1118
RsaNI GTAC 2 cut(s) 247, 1117
RseI CAYNNNNRTG 1 cut(s) 821
SaqAI TTAA 1 cut(s) 1372
SatI GCNGC 1 cut(s) 352
Sau3AI GATC 8 cut(s) 54, 63, 665, 753, 787, 883, 1239, 1279
Sau96I GGNCC 6 cut(s) 442, 462, 473, 695, 1069, 1070
SchI GAGTC 2 cut(s) 703, 1034
ScrFI CCNGG 4 cut(s) 348, 471, 990, 1073
SduI GDGCHC 3 cut(s) 947, 1073, 1223
SfaNI GCATC 3 cut(s) 378, 958, 1258
SfcI CTRYAG 1 cut(s) 1014
SinI GGWCC 1 cut(s) 462
SmiMI CAYNNNNRTG 1 cut(s) 821
Sse9I AATT 4 cut(s) 215, 635, 1056, 1138
SsiI CCGC 3 cut(s) 210, 361, 615
SspI AATATT 1 cut(s) 812
SspMI CTAG 6 cut(s) 110, 575, 630, 663, 875, 1083
StyD4I CCNGG 4 cut(s) 346, 469, 988, 1071
StyI CCWWGG 2 cut(s) 538, 1143
TaaI ACNGT 5 cut(s) 391, 533, 674, 919, 934
TaiI ACGT 2 cut(s) 1066, 1232
TaqI TCGA 2 cut(s) 53, 1232
TasI AATT 4 cut(s) 215, 635, 1056, 1138
TatI WGTACW 2 cut(s) 246, 1116
TfiI GAWTC 1 cut(s) 588
Tru1I TTAA 1 cut(s) 1372
Tru9I TTAA 1 cut(s) 1372
TseFI GTSAC 1 cut(s) 855
TseI GCWGC 1 cut(s) 351
Tsp45I GTSAC 1 cut(s) 855
TspDTI ATGAA 3 cut(s) 8, 490, 805
VpaK11BI GGWCC 1 cut(s) 462
XapI RAATTY 2 cut(s) 635, 1138
XspI CTAG 6 cut(s) 110, 575, 630, 663, 875, 1083
ZraI GACGTC 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.