Rh4BG218400

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000716
Physical Location & Seq
Forward (+)
37866132 .. 37875402
9271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG218400.1

Sequence Viewer

Length: 894 bp
ATGTTTGAACCTGCTGGAGCTAAAACTGGAAAACGCGTAAATAATACATTAATATACATGCCATGCGGAACCCACGTTTCCTTATGCTCATACAGTGATATCCAGCTGCACCCATATTGGTCTCTCGTCTCTTCGACTTGGAATGGAAAAAACTTCGCTGACCAGCCGTGGAAAATTCGCCGTTTGCTGCCCTCCTCAAGAAGATCATACGGTGTCGTATTCTTCGGGGACGGTCCTTATAATCTGCTGCCCGGTATTGATGTATCTAAGTCTCTTATCTACACCCCGTTGATCGTCAACCCTGTAACTACTGGGGCTAATTATGAAGAAAGCGAGCCTTCCTCAGAATATTTCATCAACGTAACGTCAATCAAGATCAACGGAAAGGTTGTTTCCGGATTCAACACTTCCTTGCTGACAATCGACAAAGATGGGTATGGAGGCACAAAGATTAGCAGCGTCAATCCTTACACAGTCATGGAGACCTCGATCTACAACGCTTTCTTTGATCTCTTTACTAAAGAACTCTCTGGAGTGCCAAGAGTGACACCCGTAGAACCATTCGGAGTGTGCTTCAACTCAACCAATCTCGGTAGTACTCGGGTTGGTCCGCCGGTGCCTACCATTGATCTGGTGTTGCAGAGCGAGAGCGTTTACTGGAGGATCTTTGGGGCAAACTCGATGGTGCAGGTGAGCCAGGACGTGCTGTGCTTAGGGTTTGTGGATAACGGTCAGAGCCCGAGTAACCCTAGAACTAATATTGTGATTGGTGCGCATCAAATTGAGGATAATCTTCTTCAGTTTGATTTGGCTTCTTCTAGGCTGGGATTCAGCTCCTCTCTCTTGTTCCGACAGACCACTTGCGCTAACTTCAACTTCACTTCTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

297

Amino Acids

32.58

Weight (kDa)

6.3

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_C PF14541 116 - 278 8.4e-59 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AarI CACCTGC 1 cut(s) 679
Acc16I TGCGCA 1 cut(s) 774
Acc36I ACCTGC 2 cut(s) 19, 679
AccB1I GGYRCC 1 cut(s) 616
AccII CGCG 1 cut(s) 36
AccIII TCCGGA 1 cut(s) 395
AciI CCGC 2 cut(s) 66, 611
AclWI GGATC 1 cut(s) 671
AcsI RAATTY 1 cut(s) 174
AcuI CTGAAG 1 cut(s) 782
AfaI GTAC 1 cut(s) 598
AflIII ACRYGT 1 cut(s) 34
AgsI TTSAA 4 cut(s) 8, 403, 577, 874
AjiI CACGTC 1 cut(s) 703
AjnI CCWGG 1 cut(s) 696
AluBI AGCT 3 cut(s) 20, 106, 834
AluI AGCT 3 cut(s) 20, 106, 834
Alw26I GTCTC 4 cut(s) 126, 133, 276, 476
AlwI GGATC 1 cut(s) 671
Ama87I CYCGRG 2 cut(s) 600, 739
Aor13HI TCCGGA 1 cut(s) 395
ApeKI GCWGC 5 cut(s) 106, 187, 247, 456, 887
ApoI RAATTY 1 cut(s) 174
AseI ATTAAT 1 cut(s) 50
AspLEI GCGC 2 cut(s) 775, 866
AspS9I GGNCC 2 cut(s) 233, 608
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 703
AvaI CYCGRG 2 cut(s) 600, 739
AvaII GGWCC 2 cut(s) 233, 608
BanI GGYRCC 1 cut(s) 616
BanII GRGCYC 1 cut(s) 740
BbvI GCAGC 5 cut(s) 93, 174, 234, 468, 874
BccI CCATC 2 cut(s) 425, 676
BceAI ACGGC 2 cut(s) 151, 165
BciT130I CCWGG 1 cut(s) 698
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 4 cut(s) 126, 133, 276, 476
BfaI CTAG 2 cut(s) 750, 819
BfuAI ACCTGC 2 cut(s) 19, 679
BisI GCNGC 5 cut(s) 107, 188, 248, 457, 888
BlsI GCNGC 5 cut(s) 108, 189, 249, 458, 889
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 2 cut(s) 252, 698
Bme18I GGWCC 2 cut(s) 233, 608
BmeT110I CYCGRG 2 cut(s) 600, 739
BmgBI CACGTC 1 cut(s) 703
BmgT120I GGNCC 2 cut(s) 233, 608
BmiI GGNNCC 2 cut(s) 70, 618
BmrFI CCNGG 2 cut(s) 252, 698
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 784
BmuI ACTGGG 1 cut(s) 321
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 3 cut(s) 36, 552, 679
Bpu10I CCTNAGC 1 cut(s) 712
BpuEI CTTGAG 1 cut(s) 181
BpuMI CCSGG 1 cut(s) 252
BsaI GGTCTC 2 cut(s) 126, 476
BsaJI CCNNGG 1 cut(s) 167
BsaWI WCCGGW 1 cut(s) 395
Bse118I RCCGGY 1 cut(s) 613
Bse1I ACTGG 3 cut(s) 31, 316, 662
BseAI TCCGGA 1 cut(s) 395
BseBI CCWGG 1 cut(s) 698
BseDI CCNNGG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 3 cut(s) 31, 316, 662
BseRI GAGGAG 2 cut(s) 184, 826
BseXI GCAGC 5 cut(s) 93, 174, 234, 468, 874
BseYI CCCAGC 1 cut(s) 823
BsgI GTGCAG 2 cut(s) 92, 707
Bsh1236I CGCG 1 cut(s) 36
BshNI GGYRCC 1 cut(s) 616
BsiHKCI CYCGRG 2 cut(s) 600, 739
BsiSI CCGG 3 cut(s) 252, 396, 614
BslFI GGGAC 1 cut(s) 242
BsmAI GTCTC 4 cut(s) 126, 133, 276, 476
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 242
Bso31I GGTCTC 2 cut(s) 126, 476
BsoBI CYCGRG 2 cut(s) 600, 739
Bsp1286I GDGCHC 1 cut(s) 740
Bsp13I TCCGGA 1 cut(s) 395
Bsp143I GATC 7 cut(s) 203, 291, 375, 489, 508, 628, 663
BspACI CCGC 2 cut(s) 66, 611
BspCNI CTCAG 1 cut(s) 356
BspEI TCCGGA 1 cut(s) 395
BspFNI CGCG 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 70, 618
BspMI ACCTGC 2 cut(s) 19, 679
BspPI GGATC 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 616
BspTNI GGTCTC 2 cut(s) 126, 476
BsrFI RCCGGY 1 cut(s) 613
BsrI ACTGG 3 cut(s) 31, 316, 662
BssAI RCCGGY 1 cut(s) 613
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 7 cut(s) 203, 291, 375, 489, 508, 628, 663
Bst2UI CCWGG 1 cut(s) 698
Bst4CI ACNGT 5 cut(s) 95, 212, 233, 475, 731
Bst6I CTCTTC 1 cut(s) 136
BstC8I GCNNGC 1 cut(s) 335
BstDEI CTNAG 4 cut(s) 267, 343, 712, 891
BstDSI CCRYGG 1 cut(s) 167
BstFNI CGCG 1 cut(s) 36
BstHHI GCGC 2 cut(s) 775, 866
BstKTI GATC 7 cut(s) 206, 294, 378, 492, 511, 631, 666
BstMAI GTCTC 4 cut(s) 126, 133, 276, 476
BstMBI GATC 7 cut(s) 203, 291, 375, 489, 508, 628, 663
BstNI CCWGG 1 cut(s) 698
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 2 cut(s) 250, 696
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 5 cut(s) 93, 174, 234, 468, 874
BstX2I RGATCY 1 cut(s) 663
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 1 cut(s) 663
BtgI CCRYGG 1 cut(s) 167
BtrI CACGTC 1 cut(s) 703
BtsIMutI CAGTG 1 cut(s) 100
BveI ACCTGC 2 cut(s) 19, 679
Cac8I GCNNGC 1 cut(s) 335
CfoI GCGC 2 cut(s) 775, 866
Cfr10I RCCGGY 1 cut(s) 613
Cfr13I GGNCC 2 cut(s) 233, 608
CseI GACGC 1 cut(s) 448
Csp6I GTAC 1 cut(s) 597
CviAII CATG 3 cut(s) 58, 63, 478
CviQI GTAC 1 cut(s) 597
DdeI CTNAG 4 cut(s) 267, 343, 712, 891
DpnI GATC 7 cut(s) 205, 293, 377, 491, 510, 630, 665
DpnII GATC 7 cut(s) 203, 291, 375, 489, 508, 628, 663
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
EciI GGCGGA 1 cut(s) 600
Eco24I GRGCYC 1 cut(s) 740
Eco31I GGTCTC 2 cut(s) 126, 476
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 233, 608
Eco57I CTGAAG 1 cut(s) 782
Eco88I CYCGRG 2 cut(s) 600, 739
EcoRII CCWGG 1 cut(s) 696
EcoRV GATATC 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 740
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 3 cut(s) 61, 66, 481
FalI AAGNNNNNCTT 2 cut(s) 322, 354
FaqI GGGAC 1 cut(s) 242
FatI CATG 3 cut(s) 57, 62, 477
Fnu4HI GCNGC 5 cut(s) 107, 188, 248, 457, 888
FriOI GRGCYC 1 cut(s) 740
Fsp4HI GCNGC 5 cut(s) 107, 188, 248, 457, 888
FspAI RTGCGCAY 1 cut(s) 774
FspBI CTAG 2 cut(s) 750, 819
FspI TGCGCA 1 cut(s) 774
GlaI GCGC 2 cut(s) 774, 865
GluI GCNGC 5 cut(s) 107, 188, 248, 457, 888
GsaI CCCAGC 1 cut(s) 827
GsuI CTGGAG 3 cut(s) 36, 552, 679
HapII CCGG 3 cut(s) 252, 396, 614
HgaI GACGC 1 cut(s) 448
HhaI GCGC 2 cut(s) 775, 866
Hin1II CATG 3 cut(s) 61, 66, 481
Hin6I GCGC 2 cut(s) 773, 864
HinP1I GCGC 2 cut(s) 773, 864
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 2 cut(s) 399, 828
HpaII CCGG 3 cut(s) 252, 396, 614
HphI GGTGA 1 cut(s) 703
Hpy166II GTNNAC 2 cut(s) 298, 655
Hpy188I TCNGA 4 cut(s) 346, 566, 735, 851
Hpy188III TCNNGA 4 cut(s) 198, 373, 396, 531
Hpy8I GTNNAC 2 cut(s) 298, 655
HpyAV CCTTC 1 cut(s) 348
HpyCH4III ACNGT 5 cut(s) 95, 212, 233, 475, 731
HpyCH4IV ACGT 4 cut(s) 75, 360, 365, 702
HpyCH4V TGCA 3 cut(s) 109, 640, 688
HpyF3I CTNAG 4 cut(s) 267, 343, 712, 891
HpySE526I ACGT 4 cut(s) 75, 360, 365, 702
Hsp92II CATG 3 cut(s) 61, 66, 481
HspAI GCGC 2 cut(s) 773, 864
Kpn2I TCCGGA 1 cut(s) 395
Kzo9I GATC 7 cut(s) 203, 291, 375, 489, 508, 628, 663
LmnI GCTCC 2 cut(s) 17, 839
Lsp1109I GCAGC 5 cut(s) 93, 174, 234, 468, 874
LweI GCATC 1 cut(s) 784
MaeI CTAG 2 cut(s) 750, 819
MaeII ACGT 4 cut(s) 75, 360, 365, 702
MaeIII GTNAC 4 cut(s) 304, 361, 544, 743
MalI GATC 7 cut(s) 205, 293, 377, 491, 510, 630, 665
MboI GATC 7 cut(s) 203, 291, 375, 489, 508, 628, 663
MboII GAAGA 7 cut(s) 123, 213, 214, 338, 785, 788, 807
MflI RGATCY 1 cut(s) 663
MhlI GDGCHC 1 cut(s) 740
MluCI AATT 3 cut(s) 174, 319, 780
MluI ACGCGT 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 874
MnlI CCTC 8 cut(s) 202, 205, 352, 434, 496, 654, 778, 847
MroI TCCGGA 1 cut(s) 395
MseI TTAA 1 cut(s) 50
MslI CAYNNNNRTG 1 cut(s) 476
MspA1I CMGCKG 1 cut(s) 106
MspI CCGG 3 cut(s) 252, 396, 614
MspR9I CCNGG 2 cut(s) 252, 698
MvaI CCWGG 1 cut(s) 698
MvnI CGCG 1 cut(s) 36
NciI CCSGG 1 cut(s) 252
NdeII GATC 7 cut(s) 203, 291, 375, 489, 508, 628, 663
NlaIII CATG 3 cut(s) 61, 66, 481
NlaIV GGNNCC 2 cut(s) 70, 618
NmuCI GTSAC 1 cut(s) 544
NsbI TGCGCA 1 cut(s) 774
NspI RCATGY 1 cut(s) 61
PaqCI CACCTGC 1 cut(s) 679
PfeI GAWTC 2 cut(s) 399, 828
PkrI GCNGC 5 cut(s) 108, 189, 249, 458, 889
PshBI ATTAAT 1 cut(s) 50
PsiI TTATAA 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 696
PspFI CCCAGC 1 cut(s) 823
PspGI CCWGG 1 cut(s) 696
PspN4I GGNNCC 2 cut(s) 70, 618
PspPI GGNCC 2 cut(s) 233, 608
PsuI RGATCY 1 cut(s) 663
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 1 cut(s) 598
RsaNI GTAC 1 cut(s) 597
RseI CAYNNNNRTG 1 cut(s) 476
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 5 cut(s) 107, 188, 248, 457, 888
Sau3AI GATC 7 cut(s) 203, 291, 375, 489, 508, 628, 663
Sau96I GGNCC 2 cut(s) 233, 608
ScaI AGTACT 1 cut(s) 598
ScrFI CCNGG 2 cut(s) 252, 698
SduI GDGCHC 1 cut(s) 740
SfaNI GCATC 1 cut(s) 784
SgrAI CRCCGGYG 1 cut(s) 613
SinI GGWCC 2 cut(s) 233, 608
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
Sse9I AATT 3 cut(s) 174, 319, 780
SsiI CCGC 2 cut(s) 66, 611
SspI AATATT 2 cut(s) 350, 760
SspMI CTAG 2 cut(s) 750, 819
StyD4I CCNGG 2 cut(s) 250, 696
TaaI ACNGT 5 cut(s) 95, 212, 233, 475, 731
TaiI ACGT 4 cut(s) 78, 363, 368, 705
TaqI TCGA 4 cut(s) 134, 423, 488, 680
TasI AATT 3 cut(s) 174, 319, 780
TatI WGTACW 1 cut(s) 596
TfiI GAWTC 2 cut(s) 399, 828
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 5 cut(s) 106, 187, 247, 456, 887
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 2 cut(s) 339, 343
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 233, 608
VspI ATTAAT 1 cut(s) 50
XapI RAATTY 1 cut(s) 174
XceI RCATGY 1 cut(s) 61
XspI CTAG 2 cut(s) 750, 819
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.