Rh4CG223500

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000716
Physical Location & Seq
Reverse (-)
46023068 .. 46024060
993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG223500.1

Sequence Viewer

Length: 993 bp
ATGGCTTCCTCATCATTTCTTTGCCTTACTCTCCTTTCCTCACTCGTCTTCTTCATCGCCACTCCATCACTAGCCAAAACATCTTTCAGGCCCAAAGCCCTCGTTCTTCCGGTAGCCAAACACTCTCCCACTCACCAATACCTCACCAGCATCAAGCAAAGAACCCCTCTTGTTCCTGTTAGACTAACCCTAGACCTCGGCGGTCAGTTCCTTTGGGTAGACTGTCAACAAGGCTATGTCTCATCTACCTACAAACCTGCCCGTTGCAACTCGGCTCAATGCTCACTCGCAAACTCTAAGGCGTGTACAACAGAGTGCAATTCTTCCCCCAGGCCAGGCTGCAACAACAACACATGTAGTGTCCTCCCAGACAACTCCGTGATCCCCACGTCCGGCAACGGCGGGGAGGTAGGTCAAGACGTCGTCTCCCTCCATTCCACAGATGGGTCTAACCCTACCACACTAGTTTCTGTTCCCAACTTCCTCTTCGCTTGTGCTGAAACTTTTCTATTGGACCGCCTTGCAAGTGGTGTCAAAGGTATGGCTGGTCTTGGTAGAGCTAAAATTGGACTTCCTTCTCTATTTGCTTCCGCTTTTAGCTTCAAAAGAAAATTCGCCATTTGCTTGCCCTCCTCAACAAAATCATACGGTGCCGTTTTCTTCGGAGATGGGCCTCACAACCTTCTTCCTGGGATTGACGTCTCTCAATCTCTTATTTACACCCCATTGCTCCTCAACCCTGTAAGCACTGCCAGTGCCTATTTTCAAGGCGATGCTTCTACGGAATACTTCATCAATGTAACGTCAATCAAGATCAACGACAAGGTCGTTTCGTCATTTAACAGTTCCTTGCTAACCATTGACGAATATGGTTATGGACTTATGGCGGCACGAAGATCAGCACGGTGCATCCCTACACGGTCTTGGAGACGTCAATTTACAACGCTGTGGTCAATGCATTTGTCAGTGAACTCTCTCATGTACCCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

330

Amino Acids

35.56

Weight (kDa)

9.13

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 47 - 223 1.5e-45 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 423, 702, 934
Acc36I ACCTGC 1 cut(s) 265
AccB1I GGYRCC 1 cut(s) 650
AccI GTMKAC 1 cut(s) 219
AciI CCGC 5 cut(s) 201, 402, 517, 591, 887
AclWI GGATC 1 cut(s) 376
AcsI RAATTY 1 cut(s) 611
AcyI GRCGYC 3 cut(s) 420, 699, 931
AfaI GTAC 2 cut(s) 307, 983
AfiI CCNNNNNNNGG 3 cut(s) 335, 392, 444
AflIII ACRYGT 1 cut(s) 353
AgsI TTSAA 2 cut(s) 604, 767
AhlI ACTAGT 1 cut(s) 463
AjiI CACGTC 1 cut(s) 390
AjnI CCWGG 3 cut(s) 329, 334, 688
AjuI GAANNNNNNNTTGG 4 cut(s) 68, 100, 470, 502
AluBI AGCT 2 cut(s) 560, 600
AluI AGCT 2 cut(s) 560, 600
Alw26I GTCTC 4 cut(s) 244, 430, 706, 922
AlwI GGATC 1 cut(s) 376
AoxI GGCC 3 cut(s) 89, 332, 671
ApeKI GCWGC 1 cut(s) 339
ApoI RAATTY 1 cut(s) 611
Asp700I GAANNNNTTC 1 cut(s) 504
AspS9I GGNCC 3 cut(s) 90, 514, 671
AsuHPI GGTGA 2 cut(s) 125, 136
AvaII GGWCC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 650
BbsI GAAGAC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 326
BccI CCATC 3 cut(s) 73, 437, 662
BceAI ACGGC 2 cut(s) 415, 638
BciT130I CCWGG 3 cut(s) 331, 336, 690
BcoDI GTCTC 4 cut(s) 244, 430, 706, 922
BcuI ACTAGT 1 cut(s) 463
BfaI CTAG 3 cut(s) 71, 191, 464
BfuAI ACCTGC 1 cut(s) 265
BisI GCNGC 2 cut(s) 340, 888
BlsI GCNGC 2 cut(s) 341, 889
Bme1390I CCNGG 3 cut(s) 331, 336, 690
Bme18I GGWCC 1 cut(s) 514
BmgBI CACGTC 1 cut(s) 390
BmgT120I GGNCC 3 cut(s) 90, 514, 671
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 3 cut(s) 331, 336, 690
BmsI GCATC 3 cut(s) 159, 763, 918
BpiI GAAGAC 1 cut(s) 40
BsaHI GRCGYC 3 cut(s) 420, 699, 931
BsaJI CCNNGG 3 cut(s) 196, 329, 689
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 3 cut(s) 335, 392, 444
Bse1I ACTGG 1 cut(s) 753
Bse3DI GCAATG 1 cut(s) 725
BseBI CCWGG 3 cut(s) 331, 336, 690
BseDI CCNNGG 3 cut(s) 196, 329, 689
BseGI GGATG 1 cut(s) 909
BseLI CCNNNNNNNGG 3 cut(s) 335, 392, 444
BseMI GCAATG 1 cut(s) 725
BseNI ACTGG 1 cut(s) 753
BseRI GAGGAG 2 cut(s) 622, 722
BseXI GCAGC 1 cut(s) 326
BshFI GGCC 3 cut(s) 91, 334, 673
BshNI GGYRCC 1 cut(s) 650
BsiSI CCGG 2 cut(s) 110, 393
BslI CCNNNNNNNGG 3 cut(s) 335, 392, 444
BsmAI GTCTC 4 cut(s) 244, 430, 706, 922
BsmBI CGTCTC 3 cut(s) 430, 706, 922
BsnI GGCC 3 cut(s) 91, 334, 673
Bsp1407I TGTACA 1 cut(s) 305
Bsp143I GATC 3 cut(s) 381, 813, 896
BspACI CCGC 5 cut(s) 201, 402, 517, 591, 887
BspANI GGCC 3 cut(s) 91, 334, 673
BspLI GGNNCC 1 cut(s) 652
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 725
BsrGI TGTACA 1 cut(s) 305
BsrI ACTGG 1 cut(s) 753
BssECI CCNNGG 3 cut(s) 196, 329, 689
BssMI GATC 3 cut(s) 381, 813, 896
BssNI GRCGYC 3 cut(s) 420, 699, 931
Bst2UI CCWGG 3 cut(s) 331, 336, 690
Bst4CI ACNGT 5 cut(s) 224, 650, 845, 906, 921
Bst6I CTCTTC 1 cut(s) 491
BstACI GRCGYC 3 cut(s) 420, 699, 931
BstAUI TGTACA 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 626
BstDEI CTNAG 1 cut(s) 297
BstF5I GGATG 1 cut(s) 909
BstKTI GATC 3 cut(s) 384, 816, 899
BstMAI GTCTC 4 cut(s) 244, 430, 706, 922
BstMBI GATC 3 cut(s) 381, 813, 896
BstNI CCWGG 3 cut(s) 331, 336, 690
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 3 cut(s) 329, 334, 688
BstV1I GCAGC 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 40
BsuRI GGCC 3 cut(s) 91, 334, 673
BtgZI GCGATG 2 cut(s) 40, 786
BtrI CACGTC 1 cut(s) 390
BtsCI GGATG 1 cut(s) 909
BtsI GCAGTG 1 cut(s) 747
BtsIMutI CAGTG 3 cut(s) 747, 760, 972
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 1 cut(s) 626
Cfr13I GGNCC 3 cut(s) 90, 514, 671
Csp6I GTAC 2 cut(s) 306, 982
CviAII CATG 2 cut(s) 354, 979
CviQI GTAC 2 cut(s) 306, 982
DdeI CTNAG 1 cut(s) 297
DpnI GATC 3 cut(s) 383, 815, 898
DpnII GATC 3 cut(s) 381, 813, 896
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
Eco47I GGWCC 1 cut(s) 514
EcoRII CCWGG 3 cut(s) 329, 334, 688
EcoT22I ATGCAT 1 cut(s) 960
Esp3I CGTCTC 3 cut(s) 430, 706, 922
FaeI CATG 2 cut(s) 357, 982
FaiI YATR 8 cut(s) 237, 355, 542, 646, 870, 876, 884, 980
FatI CATG 2 cut(s) 353, 978
FauI CCCGC 1 cut(s) 395
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 2 cut(s) 340, 888
FokI GGATG 1 cut(s) 896
Fsp4HI GCNGC 2 cut(s) 340, 888
FspBI CTAG 3 cut(s) 71, 191, 464
GluI GCNGC 2 cut(s) 340, 888
HaeIII GGCC 3 cut(s) 91, 334, 673
HapII CCGG 2 cut(s) 110, 393
Hin1I GRCGYC 3 cut(s) 420, 699, 931
Hin1II CATG 2 cut(s) 357, 982
HincII GTYRAC 1 cut(s) 227
HindII GTYRAC 1 cut(s) 227
HpaII CCGG 2 cut(s) 110, 393
HphI GGTGA 2 cut(s) 125, 136
Hpy166II GTNNAC 4 cut(s) 220, 227, 306, 970
Hpy188I TCNGA 1 cut(s) 665
Hpy188III TCNNGA 2 cut(s) 416, 811
Hpy8I GTNNAC 4 cut(s) 220, 227, 306, 970
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 2 cut(s) 585, 692
HpyCH4III ACNGT 5 cut(s) 224, 650, 845, 906, 921
HpyCH4IV ACGT 5 cut(s) 389, 420, 699, 803, 931
HpyCH4V TGCA 6 cut(s) 267, 318, 342, 524, 909, 958
HpyF3I CTNAG 1 cut(s) 297
HpySE526I ACGT 5 cut(s) 389, 420, 699, 803, 931
Hsp92I GRCGYC 3 cut(s) 420, 699, 931
Hsp92II CATG 2 cut(s) 357, 982
Kzo9I GATC 3 cut(s) 381, 813, 896
LmnI GCTCC 1 cut(s) 735
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 3 cut(s) 159, 763, 918
MaeI CTAG 3 cut(s) 71, 191, 464
MaeII ACGT 5 cut(s) 389, 420, 699, 803, 931
MaeIII GTNAC 1 cut(s) 799
MalI GATC 3 cut(s) 383, 815, 898
MboI GATC 3 cut(s) 381, 813, 896
MboII GAAGA 8 cut(s) 40, 43, 98, 315, 478, 652, 677, 906
MluCI AATT 4 cut(s) 319, 564, 611, 935
Mph1103I ATGCAT 1 cut(s) 960
MroXI GAANNNNTTC 1 cut(s) 504
MseI TTAA 1 cut(s) 840
MspI CCGG 2 cut(s) 110, 393
MspR9I CCNGG 3 cut(s) 331, 336, 690
MvaI CCWGG 3 cut(s) 331, 336, 690
NdeII GATC 3 cut(s) 381, 813, 896
NlaIII CATG 2 cut(s) 357, 982
NlaIV GGNNCC 1 cut(s) 652
NmeAIII GCCGAG 2 cut(s) 177, 251
NsiI ATGCAT 1 cut(s) 960
NspI RCATGY 1 cut(s) 357
PciI ACATGT 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 825
PdmI GAANNNNTTC 1 cut(s) 504
PflFI GACNNNGTC 2 cut(s) 422, 824
PkrI GCNGC 2 cut(s) 341, 889
PscI ACATGT 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 329, 334, 688
PspGI CCWGG 3 cut(s) 329, 334, 688
PspN4I GGNNCC 1 cut(s) 652
PspPI GGNCC 3 cut(s) 90, 514, 671
PsyI GACNNNGTC 2 cut(s) 422, 824
RsaI GTAC 2 cut(s) 307, 983
RsaNI GTAC 2 cut(s) 306, 982
SaqAI TTAA 1 cut(s) 840
SatI GCNGC 2 cut(s) 340, 888
Sau3AI GATC 3 cut(s) 381, 813, 896
Sau96I GGNCC 3 cut(s) 90, 514, 671
ScrFI CCNGG 3 cut(s) 331, 336, 690
SfaNI GCATC 3 cut(s) 159, 763, 918
SinI GGWCC 1 cut(s) 514
SpeI ACTAGT 1 cut(s) 463
Sse9I AATT 4 cut(s) 319, 564, 611, 935
SsiI CCGC 5 cut(s) 201, 402, 517, 591, 887
SspMI CTAG 3 cut(s) 71, 191, 464
StyD4I CCNGG 3 cut(s) 329, 334, 688
TaaI ACNGT 5 cut(s) 224, 650, 845, 906, 921
TaiI ACGT 5 cut(s) 392, 423, 702, 806, 934
TasI AATT 4 cut(s) 319, 564, 611, 935
TatI WGTACW 1 cut(s) 305
TauI GCSGC 1 cut(s) 890
Tru1I TTAA 1 cut(s) 840
Tru9I TTAA 1 cut(s) 840
TscAI CASTG 3 cut(s) 754, 760, 972
TseI GCWGC 1 cut(s) 339
TspDTI ATGAA 2 cut(s) 43, 781
TspGWI ACGGA 2 cut(s) 367, 797
TspRI CASTG 3 cut(s) 754, 760, 972
Tth111I GACNNNGTC 2 cut(s) 422, 824
VpaK11BI GGWCC 1 cut(s) 514
XapI RAATTY 1 cut(s) 611
XceI RCATGY 1 cut(s) 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 504
XspI CTAG 3 cut(s) 71, 191, 464
ZraI GACGTC 3 cut(s) 421, 700, 932
Zsp2I ATGCAT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.