Prupe.1G152100_v2.0.a1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000716
Physical Location & Seq
Forward (+)
12014640 .. 12016203
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G152100.1

Sequence Viewer

Length: 1320 bp
ATGGCTTCCTCATTTCATTGCCTTCTCTTTTGCTCACTGGTCCTCCAAATCATCACCACCACCCCCTCACTAGCCAAAGCTGCATCTTTTAGACCCAAGGCCCTTGTTCTTCCAGTCACCAAAGACCCTTCCACACTCCAATACCTCACCACCTTAAGCCAAAGAACCCCTCTTGTCCCCATAAAACTCACTGTCGATCTTGGCGGTCAATACCTTTGGGTTGACTGCGAAGAAGGCTACGTCTCATCCACCTACAAGCCTGCTCGTTGCAACTCTGCTCAGTGCTCATTGTCAAACTCCAAGGCTTGTACGACAGAGTGCTACTCTTCCCCCAAGCCAGGCTGCAACAACAACACATGCAGTCTCTCCCCCGATAACACCGTCACTAGAACCGGCACGAGCGGTGACCTTGGCCAGGACATTGTGTCACTCCAATCCACAGATGGGTTTACTCCTGGTAGGGTTGTTTCTGTCCCCAATTTGCTCTTCACTTGCGGTTCCACTTTTCTGTTGGATGGCCTGGCCAATGGTGTCAAGGGCATGGCTGGCCTTGGCAGGGCTAAAATTGGGCTTCCTTCCCAATTTGCTTCCGCTTTTAGCTTCCATCGAAAATTCGCAATTTGCTTGACCTCTTCAACAAATTCTAATGGTATTGTCATCTTCGGGGACGGGCCTTATAGTCTGCTTCCCGGAATTGATGTCTCCGAGCAACTTATTTTCACCCCATTGATCCTCAACCCTGTTAGCACTGCAAGTGCTTACTTCCAAGGAGAGCCTTCTACAGAATACTTCATCAATGTGAAGTCAATCAAGATCAACGACAAGGCCGTTGCTTTGAACACTTCATTGCTGAAAATTGACAGTGAAGGGTATGGCGGCACAAAGATTAGCACTGTGAATCCATACACAGTCTTGGAGACCTCAATCTACAACGCCGTGGTCGATGCATTTGTGAAAACAGTTGCTGAAATCCCAAGAGTGAAGGCAGTAGCACCATTTGGAGCATGCTTCGATTCAAAGAACATTGGAAGCACAAGGGTTGGGCCTGCAGTGCCTGCTATTGACCTAGTGTTGCAGAGTGAGAGTGTGTACTGGAGGGTGTTTGGAGCAAATTCGATGGTGGCGGTGAGCGATGACGTGTTGTGTTTGGGGTTTGTGGATGGAGGTGAGAGGCCAAGGACTTCAATTGTGATCGGAGGGCATCAGTTGGAGGACAATCTTCTGCAGTTTGATTTGGCTGCTTCTAGGCTTGGGTTCAGCTCCTCACTGTTCTTCAGACGAACAACTTGTGCCAACTTCAACTTCACATCTACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

440

Amino Acids

46.73

Weight (kDa)

7.4

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 402
AciI CCGC 6 cut(s) 204, 402, 495, 591, 876, 1124
AclWI GGATC 1 cut(s) 724
AcoI YGGCCR 2 cut(s) 412, 522
AcsI RAATTY 3 cut(s) 611, 640, 1111
AcuI CTGAAG 1 cut(s) 1258
AfaI GTAC 2 cut(s) 310, 1091
AfiI CCNNNNNNNGG 4 cut(s) 338, 415, 444, 556
AflII CTTAAG 1 cut(s) 154
AflIII ACRYGT 1 cut(s) 1137
AgsI TTSAA 5 cut(s) 636, 838, 1017, 1185, 1300
AhdI GACNNNNNGTC 1 cut(s) 424
AjiI CACGTC 1 cut(s) 1138
AjnI CCWGG 4 cut(s) 337, 414, 454, 519
AjuI GAANNNNNNNTTGG 4 cut(s) 470, 502, 1286, 1318
AluBI AGCT 3 cut(s) 80, 600, 1260
AluI AGCT 3 cut(s) 80, 600, 1260
Alw21I GWGCWC 1 cut(s) 287
Alw26I GTCTC 4 cut(s) 247, 368, 706, 911
AlwI GGATC 1 cut(s) 724
AlwNI CAGNNNCTG 2 cut(s) 965, 1055
AoxI GGCC 9 cut(s) 99, 412, 517, 522, 547, 671, 825, 1043, 1172
ApeKI GCWGC 3 cut(s) 80, 342, 1238
ApoI RAATTY 3 cut(s) 611, 640, 1111
AspS9I GGNCC 4 cut(s) 40, 100, 671, 1043
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 7 cut(s) 46, 109, 139, 416, 712, 1138, 1178
AvaII GGWCC 1 cut(s) 40
BalI TGGCCA 2 cut(s) 414, 524
BauI CACGAG 1 cut(s) 397
Bbv12I GWGCWC 1 cut(s) 287
BbvI GCAGC 3 cut(s) 67, 329, 1225
BccI CCATC 5 cut(s) 437, 509, 612, 1111, 1154
BceAI ACGGC 2 cut(s) 812, 920
BciT130I CCWGG 4 cut(s) 339, 416, 456, 521
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 4 cut(s) 247, 368, 706, 911
BfaI CTAG 4 cut(s) 71, 387, 1067, 1245
BfmI CTRYAG 3 cut(s) 780, 1047, 1223
BfrI CTTAAG 1 cut(s) 154
BisI GCNGC 4 cut(s) 81, 343, 877, 1239
BlsI GCNGC 4 cut(s) 82, 344, 878, 1240
Bme1390I CCNGG 5 cut(s) 339, 416, 456, 521, 690
Bme18I GGWCC 1 cut(s) 40
BmeRI GACNNNNNGTC 1 cut(s) 424
BmgBI CACGTC 1 cut(s) 1138
BmgT120I GGNCC 4 cut(s) 40, 100, 671, 1043
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 5 cut(s) 339, 416, 456, 521, 690
BmsI GCATC 3 cut(s) 92, 934, 1210
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 1 cut(s) 1114
BpuMI CCSGG 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 911
BsaJI CCNNGG 7 cut(s) 96, 300, 409, 550, 766, 936, 1175
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 4 cut(s) 338, 415, 444, 556
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 3 cut(s) 42, 113, 1097
Bse3DI GCAATG 2 cut(s) 16, 845
BseBI CCWGG 4 cut(s) 339, 416, 456, 521
BseDI CCNNGG 7 cut(s) 96, 300, 409, 550, 766, 936, 1175
BseGI GGATG 3 cut(s) 245, 520, 1165
BseLI CCNNNNNNNGG 4 cut(s) 338, 415, 444, 556
BseMI GCAATG 2 cut(s) 16, 845
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 3 cut(s) 42, 113, 1097
BseRI GAGGAG 1 cut(s) 1252
BseXI GCAGC 3 cut(s) 67, 329, 1225
BshFI GGCC 9 cut(s) 101, 414, 519, 524, 549, 673, 827, 1045, 1174
BsiHKAI GWGCWC 1 cut(s) 287
BsiSI CCGG 2 cut(s) 393, 690
BslFI GGGAC 3 cut(s) 161, 458, 680
BslI CCNNNNNNNGG 4 cut(s) 338, 415, 444, 556
BsmAI GTCTC 4 cut(s) 247, 368, 706, 911
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 3 cut(s) 161, 458, 680
BsnI GGCC 9 cut(s) 101, 414, 519, 524, 549, 673, 827, 1045, 1174
Bso31I GGTCTC 1 cut(s) 911
Bsp1286I GDGCHC 1 cut(s) 287
Bsp143I GATC 4 cut(s) 196, 729, 813, 1191
BspACI CCGC 6 cut(s) 204, 402, 495, 591, 876, 1124
BspANI GGCC 9 cut(s) 101, 414, 519, 524, 549, 673, 827, 1045, 1174
BspCNI CTCAG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 499
BspMAI CTGCAG 2 cut(s) 1051, 1227
BspPI GGATC 1 cut(s) 724
BspQI GCTCTTC 1 cut(s) 491
BspTI CTTAAG 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 911
BsrBI CCGCTC 1 cut(s) 402
BsrDI GCAATG 2 cut(s) 16, 845
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 3 cut(s) 42, 113, 1097
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 7 cut(s) 96, 300, 409, 550, 766, 936, 1175
BssMI GATC 4 cut(s) 196, 729, 813, 1191
BssSI CACGAG 1 cut(s) 397
BssT1I CCWWGG 6 cut(s) 96, 300, 409, 550, 766, 1175
Bst2BI CACGAG 1 cut(s) 397
Bst2UI CCWGG 4 cut(s) 339, 416, 456, 521
Bst4CI ACNGT 7 cut(s) 193, 382, 863, 895, 910, 961, 1269
Bst6I CTCTTC 3 cut(s) 331, 491, 637
BstAFI CTTAAG 1 cut(s) 154
BstAPI GCANNNNNTGC 1 cut(s) 1055
BstC8I GCNNGC 5 cut(s) 261, 547, 1006, 1047, 1056
BstDEI CTNAG 2 cut(s) 279, 1317
BstDSI CCRYGG 1 cut(s) 936
BstEII GGTNACC 1 cut(s) 404
BstENI CCTNNNNNAGG 1 cut(s) 413
BstF5I GGATG 3 cut(s) 245, 520, 1165
BstKTI GATC 4 cut(s) 199, 732, 816, 1194
BstMAI GTCTC 4 cut(s) 247, 368, 706, 911
BstMBI GATC 4 cut(s) 196, 729, 813, 1191
BstMWI GCNNNNNNNGC 5 cut(s) 80, 234, 546, 1051, 1055
BstNI CCWGG 4 cut(s) 339, 416, 456, 521
BstNSI RCATGY 2 cut(s) 360, 1008
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 5 cut(s) 337, 414, 454, 519, 688
BstSFI CTRYAG 3 cut(s) 780, 1047, 1223
BstV1I GCAGC 3 cut(s) 67, 329, 1225
BsuRI GGCC 9 cut(s) 101, 414, 519, 524, 549, 673, 827, 1045, 1174
BtgI CCRYGG 1 cut(s) 936
BtgZI GCGATG 1 cut(s) 1146
BtrI CACGTC 1 cut(s) 1138
BtsCI GGATG 3 cut(s) 245, 520, 1165
BtsI GCAGTG 2 cut(s) 747, 1056
BtsIMutI CAGTG 8 cut(s) 35, 189, 287, 747, 868, 891, 1056, 1265
Cac8I GCNNGC 5 cut(s) 261, 547, 1006, 1047, 1056
CaiI CAGNNNCTG 2 cut(s) 965, 1055
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 4 cut(s) 40, 100, 671, 1043
Csp6I GTAC 2 cut(s) 309, 1090
CviAII CATG 3 cut(s) 357, 541, 1005
CviQI GTAC 2 cut(s) 309, 1090
DdeI CTNAG 2 cut(s) 279, 1317
DpnI GATC 4 cut(s) 198, 731, 815, 1193
DpnII GATC 4 cut(s) 196, 729, 813, 1191
DriI GACNNNNNGTC 1 cut(s) 424
EaeI YGGCCR 2 cut(s) 412, 522
Eam1104I CTCTTC 3 cut(s) 331, 491, 637
Eam1105I GACNNNNNGTC 1 cut(s) 424
EarI CTCTTC 3 cut(s) 331, 491, 637
Eco130I CCWWGG 6 cut(s) 96, 300, 409, 550, 766, 1175
Eco31I GGTCTC 1 cut(s) 911
Eco47I GGWCC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 1258
Eco91I GGTNACC 1 cut(s) 404
EcoNI CCTNNNNNAGG 1 cut(s) 413
EcoO109I RGGNCCY 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 404
EcoRII CCWGG 4 cut(s) 337, 414, 454, 519
EcoT14I CCWWGG 6 cut(s) 96, 300, 409, 550, 766, 1175
EcoT22I ATGCAT 1 cut(s) 949
ErhI CCWWGG 6 cut(s) 96, 300, 409, 550, 766, 1175
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 3 cut(s) 360, 544, 1008
FaiI YATR 7 cut(s) 182, 358, 542, 678, 873, 904, 1006
FaqI GGGAC 3 cut(s) 161, 458, 680
FatI CATG 3 cut(s) 356, 540, 1004
Fnu4HI GCNGC 4 cut(s) 81, 343, 877, 1239
FokI GGATG 3 cut(s) 232, 527, 1172
Fsp4HI GCNGC 4 cut(s) 81, 343, 877, 1239
FspBI CTAG 4 cut(s) 71, 387, 1067, 1245
GluI GCNGC 4 cut(s) 81, 343, 877, 1239
GsuI CTGGAG 1 cut(s) 1114
HaeIII GGCC 9 cut(s) 101, 414, 519, 524, 549, 673, 827, 1045, 1174
HapII CCGG 2 cut(s) 393, 690
Hin1II CATG 3 cut(s) 360, 544, 1008
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 2 cut(s) 898, 1013
HpaII CCGG 2 cut(s) 393, 690
HphI GGTGA 7 cut(s) 46, 109, 139, 416, 712, 1138, 1178
Hpy166II GTNNAC 3 cut(s) 223, 450, 1090
Hpy188I TCNGA 3 cut(s) 706, 1196, 1277
Hpy188III TCNNGA 1 cut(s) 811
Hpy8I GTNNAC 3 cut(s) 223, 450, 1090
HpyAV CCTTC 7 cut(s) 32, 138, 227, 585, 786, 860, 976
HpyCH4III ACNGT 7 cut(s) 193, 382, 863, 895, 910, 961, 1269
HpyCH4IV ACGT 2 cut(s) 240, 1137
HpyCH4V TGCA 9 cut(s) 83, 270, 345, 360, 752, 947, 1049, 1075, 1225
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 234, 546, 1051, 1055
HpyF3I CTNAG 2 cut(s) 279, 1317
HpySE526I ACGT 2 cut(s) 240, 1137
Hsp92II CATG 3 cut(s) 360, 544, 1008
Kzo9I GATC 4 cut(s) 196, 729, 813, 1191
LguI GCTCTTC 1 cut(s) 491
LmnI GCTCC 3 cut(s) 1001, 1106, 1265
Lsp1109I GCAGC 3 cut(s) 67, 329, 1225
LweI GCATC 3 cut(s) 92, 934, 1210
MaeI CTAG 4 cut(s) 71, 387, 1067, 1245
MaeII ACGT 2 cut(s) 240, 1137
MaeIII GTNAC 4 cut(s) 115, 382, 404, 426
MalI GATC 4 cut(s) 198, 731, 815, 1193
MbiI CCGCTC 1 cut(s) 402
MboI GATC 4 cut(s) 196, 729, 813, 1191
MboII GAAGA 8 cut(s) 101, 242, 318, 478, 624, 652, 1211, 1264
MfeI CAATTG 1 cut(s) 1185
MhlI GDGCHC 1 cut(s) 287
MlsI TGGCCA 2 cut(s) 414, 524
MluNI TGGCCA 2 cut(s) 414, 524
MmeI TCCRAC 2 cut(s) 492, 1188
Mox20I TGGCCA 2 cut(s) 414, 524
Mph1103I ATGCAT 1 cut(s) 949
MscI TGGCCA 2 cut(s) 414, 524
MseI TTAA 1 cut(s) 155
MslI CAYNNNNRTG 1 cut(s) 797
Msp20I TGGCCA 2 cut(s) 414, 524
MspCI CTTAAG 1 cut(s) 154
MspI CCGG 2 cut(s) 393, 690
MspR9I CCNGG 5 cut(s) 339, 416, 456, 521, 690
MunI CAATTG 1 cut(s) 1185
MvaI CCWGG 4 cut(s) 339, 416, 456, 521
MwoI GCNNNNNNNGC 5 cut(s) 80, 234, 546, 1051, 1055
NciI CCSGG 1 cut(s) 690
NdeII GATC 4 cut(s) 196, 729, 813, 1191
NlaIII CATG 3 cut(s) 360, 544, 1008
NlaIV GGNNCC 1 cut(s) 499
NmuCI GTSAC 4 cut(s) 115, 382, 404, 426
NsiI ATGCAT 1 cut(s) 949
NspI RCATGY 2 cut(s) 360, 1008
PaeI GCATGC 1 cut(s) 1008
PciSI GCTCTTC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 2 cut(s) 825, 939
PfeI GAWTC 2 cut(s) 898, 1013
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 4 cut(s) 82, 344, 878, 1240
Psp6I CCWGG 4 cut(s) 337, 414, 454, 519
PspEI GGTNACC 1 cut(s) 404
PspGI CCWGG 4 cut(s) 337, 414, 454, 519
PspN4I GGNNCC 1 cut(s) 499
PspPI GGNCC 4 cut(s) 40, 100, 671, 1043
PstI CTGCAG 2 cut(s) 1051, 1227
PstNI CAGNNNCTG 2 cut(s) 965, 1055
RsaI GTAC 2 cut(s) 310, 1091
RsaNI GTAC 2 cut(s) 309, 1090
RseI CAYNNNNRTG 1 cut(s) 797
SapI GCTCTTC 1 cut(s) 491
SaqAI TTAA 1 cut(s) 155
SatI GCNGC 4 cut(s) 81, 343, 877, 1239
Sau3AI GATC 4 cut(s) 196, 729, 813, 1191
Sau96I GGNCC 4 cut(s) 40, 100, 671, 1043
ScrFI CCNGG 5 cut(s) 339, 416, 456, 521, 690
SduI GDGCHC 1 cut(s) 287
SfaNI GCATC 3 cut(s) 92, 934, 1210
SfcI CTRYAG 3 cut(s) 780, 1047, 1223
SinI GGWCC 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
SphI GCATGC 1 cut(s) 1008
SsiI CCGC 6 cut(s) 204, 402, 495, 591, 876, 1124
SspMI CTAG 4 cut(s) 71, 387, 1067, 1245
StyD4I CCNGG 5 cut(s) 337, 414, 454, 519, 688
StyI CCWWGG 6 cut(s) 96, 300, 409, 550, 766, 1175
TaaI ACNGT 7 cut(s) 193, 382, 863, 895, 910, 961, 1269
TaiI ACGT 2 cut(s) 243, 1140
TaqI TCGA 5 cut(s) 195, 607, 942, 1011, 1115
TatI WGTACW 1 cut(s) 1089
TauI GCSGC 1 cut(s) 879
TfiI GAWTC 2 cut(s) 898, 1013
Tru1I TTAA 1 cut(s) 155
Tru9I TTAA 1 cut(s) 155
TscAI CASTG 8 cut(s) 42, 196, 287, 754, 868, 898, 1056, 1272
TseFI GTSAC 4 cut(s) 115, 382, 404, 426
TseI GCWGC 3 cut(s) 80, 342, 1238
Tsp45I GTSAC 4 cut(s) 115, 382, 404, 426
TspDTI ATGAA 3 cut(s) 5, 781, 834
TspRI CASTG 8 cut(s) 42, 196, 287, 754, 868, 898, 1056, 1272
Vha464I CTTAAG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 40
XagI CCTNNNNNAGG 1 cut(s) 413
XapI RAATTY 3 cut(s) 611, 640, 1111
XceI RCATGY 2 cut(s) 360, 1008
XcmI CCANNNNNNNNNTGG 2 cut(s) 440, 508
XspI CTAG 4 cut(s) 71, 387, 1067, 1245
Zsp2I ATGCAT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.