Rorug04G0150300

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000716
Physical Location & Seq
Forward (+)
26366474 .. 26369961
3488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0150300.1

Sequence Viewer

Length: 2745 bp
ATGGTGGTTTCTTTAGGAGGTTTACTGATCTGGGTCCTTAATATCAACAACACACAGAGAGCTGGAATTCTTGTTCACCTTGTAGCTTTATCAAATTTATGGTGTTTAGTTTCAGGACTAGGTTCTATGTCCTCCGTTGCAGTTTCTTACGGTGAGAAAGGTGCAGTTTACTGTGGCTTAAACTTAAATGGGTCTAATCTTGTGAGCTGTTATGGATCAAACTCGGCCATAACATATGGAACCCCAATTCACTTCCCATTTATTGGCCTGACTGCTGGTGATGGCTTTGTTTGTGGTCTGCTCATGGATTCTAACCAACCATATTGTTGGGGCAGCAGTGGTTATATTCAAATGGGTGTCCCTCAACCCATAATTAAAGATGCCCAATATGTAGAAATCAGTGCTGGTGATTACCATCTCTGTGGATTGAGAAAACCTCTAACAGGGAGCCTCAGAAACATGTCTTTTGTCGATTGTTGGGGCTATAACATGACCAAAAACTATGTGTTTGATGGTCAGATGCAGTCCATTACAGCAGGGTCAGAATTCAATTGTGGATTGTTTTCCCAAAACAGGACTGTGTTTTGCTGGGGTGATGAGACTAGTAGTAGGGTTATCCATTTGATCCCCGCAGACATGAGGTTTCGGAAGATTGCAGCTGGTGGGTATCATGTCTGTGGCATTTCAGAGGGTGTGAGTTCTAGAACCTTTTGTTGGGGAAGAAGCTTGGACATTGAAGAAGAAATCTCAGTGGCATATTCGGGTCAAGGCAATGTTGATTTGGCTCCGAAGGACCCAATGCTTTCAATTGTGGGAGGGAAGTTTCATGCGTGTGGAATCAAGAGCTATGACCATGGTGTCATCTGCTGGGGATTTATTATGAAGCCAAGCACACCGGTTCCCAAGGGTATCAAGGTTTATGACATTGCTGCTGGAAATTACTTCACTTGTGGCATTCGTGCTGACAAATCATTCTTGCCTGTTTGTTGGGGTCTTGGATTTCCGACTTCTCTTCCATTACCTGTTCCCCCCATGTCCTGCAGCTCTACTCCATGTGCTCCTGGCTTCTATGAACTCAGCCATGACAGCGCCTCTTGCAAAGATCCCAACTCTCACATTTGCATGCCCTGCAGCACTGGCTGTCCTGCTGAAATGTACCAAAAAACTGAATGTACATTGAAATCTGATAGACAGTGTGATTATAACTGTTCTATTTGTTCCTCAGCTGAATGCTCCTCAAACTGTTCTTCATTGTACGCCAATTCAAAGAGGAATGAAAGGTTCTGGTCTCTGCAATTGCCTGTCATTATTGCTGAGATTGCCTTTGCTATGATATTGGTCAGTGTTGTGTCTCTAACTGCAATTTTATATGTCCGCTACAAGTTACGTGACTGTCGTTGCACAGAAAAATACGTAAAGTCCACAAAGAATGGTCGCAGTGGTTCTCCTTTCCAGAAAGACATTGGCAAGATTCGTCCAGACTTGGATGATCTGAAGATAAGGCGGGCTCAGATGTTTACTTATGAGGAACTGGAAAGAGCAACTTCGGGGTTTGAAGAAGAGTCTGTTGTGGGCAAGGGAAGCTTCTCCTCAGTTTTTAGAGGTGTTTTGAAAGATGGAACAGTTGTTGCTGTCAAAAGGGCTATAATGTCTCCTAACATGCAAAAGAATTCGAAAGAGTTCCATACTGAGCTAGACTTGCTCTCCAGATTAAACCATGCTCATTTACTCAACCTGCTTGGGTATTGTGAAGAAGGTGGAGAGAGGCTTCTTGTGTATGAGTTCATGGCTCATGGATCATTGCACCAGCACCTCCATGGAAAGAACAAAGCCTTGAGAGAGCAATTGGACTGGGTTCGAAGAGTCACAATTGCAGTCCAGGCAGCTAGGGGAATTGAATACTTGCATGGTTACGCTTGCCCACCAGTGATCCATCGAGATATTAAGTCTTCAAATATCCTGATCGATGAGGAACACAATGCTCGAGTAGCTGATTTTGGCTTGTCACTATTGGGACCTGCAGATAGTAGTTCTCCATTGGCCGAGCTACCAGCGGGGACTCTAGGGTATCTTGATCCTGAGTACTACAGACTTCACTACCTGACAACCAAATCTGATGTTTATAGTTTTGGTGCTTTGCTGTTGGAGATTCTTAGTGGCAGAAAAGCCATTGATATGCAATATGAAGAATCAGGAAATATAGTTGAATGGGCAGTGCCTCTGATCAAGGCAGGAGACATATTTGCAATTTTGGATCCAGTTTTGAAACCCCCACCTGATGTTGAAGCCTTGAAAAGAATTGCAAATGTTGCTTGCAAATGTGTGAGGATGAGAGGCAAGGAGAGGCCATCAATGGATAAAGTGACTACAGCACTTGAACGAGCTCTTGCGCTGCTAATGGGCAGCCCCTGTAATGAGCAACCCATTCTGCCTACTGAGGTGGTATTGGGAAGCAGTAGATTGCACAAGAAGTCATCGCAGAGATCTTCAAACAGGTCAGTGGAAACTGATGTTATAGATGCCGAGGATCAGAGGTTTGAATTCAGAGCTCCTTCATGGATCACATTTCCGAGTGTTGCTTCTTCCCAGAGGAGGAAATCCTCGGTCTCAGATGCCGATGCTGATGGGAAGAGCTCCATGGAAGCTAGAAATATGGGGAATGGTGGTGATGGGTTGAGGAGTTTGGAGGAAGAGATTGGGCCAGCTTCACCTCAAGAGAAGTTATTCTTGCAGCATAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

914

Amino Acids

100.35

Weight (kDa)

6.74

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 518 - 788 1.7e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 521 - 791 2e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1203
AasI GACNNNNNNGTC 1 cut(s) 857
Acc36I ACCTGC 2 cut(s) 1743, 2026
AccB7I CCANNNNNTGG 1 cut(s) 263
AciI CCGC 4 cut(s) 630, 1375, 1504, 2054
AcoI YGGCCR 2 cut(s) 225, 2040
AcsI RAATTY 5 cut(s) 66, 94, 545, 1669, 2540
AcuI CTGAAG 1 cut(s) 1514
AfaI GTAC 4 cut(s) 1157, 1174, 1256, 2084
AfiI CCNNNNNNNGG 5 cut(s) 263, 443, 573, 714, 2592
AflIII ACRYGT 1 cut(s) 459
AgeI ACCGGT 1 cut(s) 895
AhlI ACTAGT 1 cut(s) 602
AjnI CCWGG 2 cut(s) 1060, 1878
AjuI GAANNNNNNNTTGG 2 cut(s) 697, 729
AloI GAACNNNNNNTCC 2 cut(s) 57, 89
Alw21I GWGCWC 4 cut(s) 1060, 2386, 2551, 2636
Alw26I GTCTC 6 cut(s) 593, 1293, 1356, 1656, 2229, 2611
AlwNI CAGNNNCTG 1 cut(s) 2409
Ama87I CYCGRG 1 cut(s) 1983
AoxI GGCC 5 cut(s) 225, 265, 2040, 2345, 2699
ApeKI GCWGC 9 cut(s) 333, 656, 929, 1041, 1131, 1883, 2392, 2403, 2731
ApoI RAATTY 5 cut(s) 66, 94, 545, 1669, 2540
AsiGI ACCGGT 1 cut(s) 895
Asp700I GAANNNNTTC 2 cut(s) 1679, 2723
AspLEI GCGC 2 cut(s) 1091, 2392
AspS9I GGNCC 4 cut(s) 34, 793, 2015, 2699
AsuHPI GGTGA 7 cut(s) 68, 164, 290, 419, 605, 2678, 2700
AsuII TTCGAA 2 cut(s) 1673, 1858
AvaI CYCGRG 1 cut(s) 1983
AvaII GGWCC 3 cut(s) 34, 793, 2015
BamHI GGATCC 1 cut(s) 2254
BanII GRGCYC 4 cut(s) 1510, 2386, 2551, 2636
BbsI GAAGAC 1 cut(s) 1941
Bbv12I GWGCWC 4 cut(s) 1060, 2386, 2551, 2636
BbvCI CCTCAGC 1 cut(s) 1222
BbvI GCAGC 8 cut(s) 345, 668, 916, 1053, 1143, 1895, 2379, 2415
BccI CCATC 8 cut(s) 275, 423, 506, 1610, 1941, 2356, 2618, 2663
BciT130I CCWGG 2 cut(s) 1062, 1880
BclI TGATCA 1 cut(s) 2223
BcoDI GTCTC 6 cut(s) 593, 1293, 1356, 1656, 2229, 2611
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 7 cut(s) 119, 603, 702, 1694, 1887, 2063, 2646
BfmI CTRYAG 5 cut(s) 1039, 1129, 2019, 2086, 2367
BfoI RGCGCY 1 cut(s) 1092
BfuAI ACCTGC 2 cut(s) 1743, 2026
BglII AGATCT 1 cut(s) 2483
BisI GCNGC 9 cut(s) 334, 657, 930, 1042, 1132, 1884, 2393, 2404, 2732
BlsI GCNGC 9 cut(s) 335, 658, 931, 1043, 1133, 1885, 2394, 2405, 2733
BmcAI AGTACT 1 cut(s) 2084
Bme1390I CCNGG 2 cut(s) 1062, 1880
Bme18I GGWCC 3 cut(s) 34, 793, 2015
BmeT110I CYCGRG 1 cut(s) 1983
BmgT120I GGNCC 4 cut(s) 34, 793, 2015, 2699
BmiI GGNNCC 8 cut(s) 35, 241, 449, 786, 795, 900, 2016, 2256
BmrFI CCNGG 2 cut(s) 1062, 1880
BmrI ACTGGG 1 cut(s) 1861
BmsI GCATC 5 cut(s) 370, 510, 2509, 2602, 2608
BmuI ACTGGG 1 cut(s) 1861
BpiI GAAGAC 1 cut(s) 1941
BplI GAGNNNNNCTC 2 cut(s) 421, 453
BpmI CTGGAG 1 cut(s) 1690
Bpu10I CCTNAGC 1 cut(s) 1222
Bpu14I TTCGAA 2 cut(s) 1673, 1858
BpuEI CTTGAG 2 cut(s) 1855, 2697
Bsa29I ATCGAT 1 cut(s) 1965
BsaAI YACGTR 2 cut(s) 1388, 1414
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 2 cut(s) 1293, 2611
BsaJI CCNNGG 6 cut(s) 853, 903, 1816, 2523, 2601, 2637
BsaWI WCCGGW 1 cut(s) 895
BsaXI ACNNNNNCTCC 2 cut(s) 1688, 1718
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 443, 573, 714, 2592
Bse118I RCCGGY 1 cut(s) 895
Bse1I ACTGG 5 cut(s) 1141, 1536, 1856, 1925, 2258
Bse3DI GCAATG 3 cut(s) 778, 924, 1799
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 2 cut(s) 1062, 1880
BseCI ATCGAT 1 cut(s) 1965
BseDI CCNNGG 6 cut(s) 853, 903, 1816, 2523, 2601, 2637
BseGI GGATG 2 cut(s) 1492, 2334
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 5 cut(s) 263, 443, 573, 714, 2592
BseMI GCAATG 3 cut(s) 778, 924, 1799
BseNI ACTGG 5 cut(s) 1141, 1536, 1856, 1925, 2258
BseRI GAGGAG 4 cut(s) 1225, 1579, 2605, 2692
BseXI GCAGC 8 cut(s) 345, 668, 916, 1053, 1143, 1895, 2379, 2415
BseYI CCCAGC 2 cut(s) 588, 867
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 5 cut(s) 227, 267, 2042, 2347, 2701
BshTI ACCGGT 1 cut(s) 895
BshVI ATCGAT 1 cut(s) 1965
BsiHKAI GWGCWC 4 cut(s) 1060, 2386, 2551, 2636
BsiHKCI CYCGRG 1 cut(s) 1983
BsiSI CCGG 1 cut(s) 896
BslFI GGGAC 3 cut(s) 344, 2028, 2071
BslI CCNNNNNNNGG 5 cut(s) 263, 443, 573, 714, 2592
BsmAI GTCTC 6 cut(s) 593, 1293, 1356, 1656, 2229, 2611
BsmFI GGGAC 3 cut(s) 344, 2028, 2071
BsmI GAATGC 2 cut(s) 954, 1235
BsnI GGCC 5 cut(s) 227, 267, 2042, 2347, 2701
Bso31I GGTCTC 2 cut(s) 1293, 2611
BsoBI CYCGRG 1 cut(s) 1983
Bsp119I TTCGAA 2 cut(s) 1673, 1858
Bsp1286I GDGCHC 5 cut(s) 1060, 1510, 2386, 2551, 2636
Bsp1407I TGTACA 1 cut(s) 1172
Bsp19I CCATGG 3 cut(s) 853, 1816, 2637
BspACI CCGC 4 cut(s) 630, 1375, 1504, 2054
BspANI GGCC 5 cut(s) 227, 267, 2042, 2347, 2701
BspDI ATCGAT 1 cut(s) 1965
BspLI GGNNCC 8 cut(s) 35, 241, 449, 786, 795, 900, 2016, 2256
BspMAI CTGCAG 3 cut(s) 1043, 1133, 2023
BspMI ACCTGC 2 cut(s) 1743, 2026
BspQI GCTCTTC 1 cut(s) 2624
BspT104I TTCGAA 2 cut(s) 1673, 1858
BspTNI GGTCTC 2 cut(s) 1293, 2611
BsrDI GCAATG 3 cut(s) 778, 924, 1799
BsrFI RCCGGY 1 cut(s) 895
BsrGI TGTACA 1 cut(s) 1172
BsrI ACTGG 5 cut(s) 1141, 1536, 1856, 1925, 2258
BssAI RCCGGY 1 cut(s) 895
BssECI CCNNGG 6 cut(s) 853, 903, 1816, 2523, 2601, 2637
BssT1I CCWWGG 4 cut(s) 853, 903, 1816, 2637
Bst2UI CCWGG 2 cut(s) 1062, 1880
Bst4CI ACNGT 8 cut(s) 152, 173, 580, 1194, 1208, 1244, 1394, 1624
Bst6I CTCTTC 5 cut(s) 1017, 1554, 1855, 2624, 2685
BstAPI GCANNNNNTGC 2 cut(s) 1128, 2309
BstAUI TGTACA 1 cut(s) 1172
BstBAI YACGTR 2 cut(s) 1388, 1414
BstBI TTCGAA 2 cut(s) 1673, 1858
BstC8I GCNNGC 5 cut(s) 1124, 1506, 1918, 2314, 2703
BstDSI CCRYGG 3 cut(s) 853, 1816, 2637
BstENI CCTNNNNNAGG 1 cut(s) 441
BstF5I GGATG 2 cut(s) 1492, 2334
BstH2I RGCGCY 1 cut(s) 1092
BstHHI GCGC 2 cut(s) 1091, 2392
BstMAI GTCTC 6 cut(s) 593, 1293, 1356, 1656, 2229, 2611
BstNI CCWGG 2 cut(s) 1062, 1880
BstNSI RCATGY 3 cut(s) 463, 1126, 1663
BstSCI CCNGG 2 cut(s) 1060, 1878
BstSFI CTRYAG 5 cut(s) 1039, 1129, 2019, 2086, 2367
BstSNI TACGTA 1 cut(s) 1414
BstV1I GCAGC 8 cut(s) 345, 668, 916, 1053, 1143, 1895, 2379, 2415
BstV2I GAAGAC 1 cut(s) 1941
BstX2I RGATCY 3 cut(s) 1102, 2254, 2483
BstXI CCANNNNNNTGG 2 cut(s) 327, 422
BstYI RGATCY 3 cut(s) 1102, 2254, 2483
Bsu15I ATCGAT 1 cut(s) 1965
BsuRI GGCC 5 cut(s) 227, 267, 2042, 2347, 2701
BsuTUI ATCGAT 1 cut(s) 1965
BtgI CCRYGG 3 cut(s) 853, 1816, 2637
BtgZI GCGATG 1 cut(s) 2460
BtsCI GGATG 2 cut(s) 1492, 2334
BtsI GCAGTG 3 cut(s) 343, 1444, 2220
BveI ACCTGC 2 cut(s) 1743, 2026
Cac8I GCNNGC 5 cut(s) 1124, 1506, 1918, 2314, 2703
CaiI CAGNNNCTG 1 cut(s) 2409
CfoI GCGC 2 cut(s) 1091, 2392
Cfr10I RCCGGY 1 cut(s) 895
Cfr13I GGNCC 4 cut(s) 34, 793, 2015, 2699
ClaI ATCGAT 1 cut(s) 1965
Csp6I GTAC 4 cut(s) 1156, 1173, 1255, 2083
CspAI ACCGGT 1 cut(s) 895
CviQI GTAC 4 cut(s) 1156, 1173, 1255, 2083
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 2 cut(s) 225, 2040
Eam1104I CTCTTC 5 cut(s) 1017, 1554, 1855, 2624, 2685
EarI CTCTTC 5 cut(s) 1017, 1554, 1855, 2624, 2685
Ecl136II GAGCTC 3 cut(s) 2384, 2549, 2634
Eco105I TACGTA 1 cut(s) 1414
Eco130I CCWWGG 4 cut(s) 853, 903, 1816, 2637
Eco24I GRGCYC 4 cut(s) 1510, 2386, 2551, 2636
Eco31I GGTCTC 2 cut(s) 1293, 2611
Eco47I GGWCC 3 cut(s) 34, 793, 2015
Eco53kI GAGCTC 3 cut(s) 2384, 2549, 2634
Eco57I CTGAAG 1 cut(s) 1514
Eco88I CYCGRG 1 cut(s) 1983
EcoICRI GAGCTC 3 cut(s) 2384, 2549, 2634
EcoNI CCTNNNNNAGG 1 cut(s) 441
EcoO109I RGGNCCY 3 cut(s) 34, 793, 2015
EcoRI GAATTC 4 cut(s) 66, 545, 1669, 2540
EcoRII CCWGG 2 cut(s) 1060, 1878
EcoT14I CCWWGG 4 cut(s) 853, 903, 1816, 2637
EcoT38I GRGCYC 4 cut(s) 1510, 2386, 2551, 2636
ErhI CCWWGG 4 cut(s) 853, 903, 1816, 2637
FalI AAGNNNNNCTT 6 cut(s) 1528, 1560, 1568, 1600, 2711, 2743
FaqI GGGAC 3 cut(s) 344, 2028, 2071
FauI CCCGC 3 cut(s) 637, 1497, 2047
FauNDI CATATG 1 cut(s) 235
FbaI TGATCA 1 cut(s) 2223
Fnu4HI GCNGC 9 cut(s) 334, 657, 930, 1042, 1132, 1884, 2393, 2404, 2732
FokI GGATG 2 cut(s) 1499, 2341
FriOI GRGCYC 4 cut(s) 1510, 2386, 2551, 2636
Fsp4HI GCNGC 9 cut(s) 334, 657, 930, 1042, 1132, 1884, 2393, 2404, 2732
FspBI CTAG 7 cut(s) 119, 603, 702, 1694, 1887, 2063, 2646
GlaI GCGC 2 cut(s) 1090, 2391
GluI GCNGC 9 cut(s) 334, 657, 930, 1042, 1132, 1884, 2393, 2404, 2732
GsaI CCCAGC 2 cut(s) 592, 871
GsuI CTGGAG 1 cut(s) 1690
HaeII RGCGCY 1 cut(s) 1092
HaeIII GGCC 5 cut(s) 227, 267, 2042, 2347, 2701
HapII CCGG 1 cut(s) 896
HhaI GCGC 2 cut(s) 1091, 2392
Hin6I GCGC 2 cut(s) 1089, 2390
HinP1I GCGC 2 cut(s) 1089, 2390
HindIII AAGCTT 2 cut(s) 724, 1582
HinfI GANTC 8 cut(s) 308, 837, 1471, 1562, 1863, 2059, 2149, 2189
HpaII CCGG 1 cut(s) 896
HphI GGTGA 7 cut(s) 68, 164, 290, 419, 605, 2678, 2700
Hpy166II GTNNAC 5 cut(s) 23, 76, 169, 1422, 1518
Hpy8I GTNNAC 5 cut(s) 23, 76, 169, 1422, 1518
HpyAV CCTTC 3 cut(s) 784, 1748, 2562
HpyCH4III ACNGT 8 cut(s) 152, 173, 580, 1194, 1208, 1244, 1394, 1624
HpyCH4IV ACGT 2 cut(s) 1387, 1413
HpySE526I ACGT 2 cut(s) 1387, 1413
HspAI GCGC 2 cut(s) 1089, 2390
Ksp22I TGATCA 1 cut(s) 2223
LguI GCTCTTC 1 cut(s) 2624
LmnI GCTCC 6 cut(s) 447, 790, 1063, 1238, 2554, 2639
Lsp1109I GCAGC 8 cut(s) 345, 668, 916, 1053, 1143, 1895, 2379, 2415
LweI GCATC 5 cut(s) 370, 510, 2509, 2602, 2608
MaeI CTAG 7 cut(s) 119, 603, 702, 1694, 1887, 2063, 2646
MaeII ACGT 2 cut(s) 1387, 1413
MaeIII GTNAC 6 cut(s) 1383, 1388, 1864, 1910, 2004, 2362
MfeI CAATTG 5 cut(s) 550, 807, 1295, 1844, 1869
MflI RGATCY 3 cut(s) 1102, 2254, 2483
MhlI GDGCHC 5 cut(s) 1060, 1510, 2386, 2551, 2636
MlyI GAGTC 3 cut(s) 1571, 1872, 2053
MmeI TCCRAC 2 cut(s) 1028, 2124
MroXI GAANNNNTTC 2 cut(s) 1679, 2723
MseI TTAA 7 cut(s) 39, 179, 185, 375, 1712, 1944, 2743
MslI CAYNNNNRTG 6 cut(s) 420, 675, 831, 1121, 1815, 2174
MspA1I CMGCKG 3 cut(s) 659, 1226, 2054
MspI CCGG 1 cut(s) 896
MspR9I CCNGG 2 cut(s) 1062, 1880
MunI CAATTG 5 cut(s) 550, 807, 1295, 1844, 1869
Mva1269I GAATGC 2 cut(s) 954, 1235
MvaI CCWGG 2 cut(s) 1062, 1880
NcoI CCATGG 3 cut(s) 853, 1816, 2637
NdeI CATATG 1 cut(s) 235
NlaIV GGNNCC 8 cut(s) 35, 241, 449, 786, 795, 900, 2016, 2256
NmeAIII GCCGAG 3 cut(s) 203, 2068, 2548
NmuCI GTSAC 4 cut(s) 1388, 1864, 2004, 2362
NspI RCATGY 3 cut(s) 463, 1126, 1663
NspV TTCGAA 2 cut(s) 1673, 1858
PaeI GCATGC 1 cut(s) 1126
PaeR7I CTCGAG 1 cut(s) 1983
PciI ACATGT 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 2624
PcsI WCGNNNNNNNCGW 1 cut(s) 1393
PctI GAATGC 2 cut(s) 954, 1235
PdmI GAANNNNTTC 2 cut(s) 1679, 2723
PfeI GAWTC 5 cut(s) 308, 837, 1471, 2149, 2189
PflMI CCANNNNNTGG 1 cut(s) 263
PinAI ACCGGT 1 cut(s) 895
PkrI GCNGC 9 cut(s) 335, 658, 931, 1043, 1133, 1885, 2394, 2405, 2733
PleI GAGTC 3 cut(s) 1570, 1871, 2053
PpsI GAGTC 3 cut(s) 1570, 1871, 2053
Ppu21I YACGTR 2 cut(s) 1388, 1414
PpuMI RGGWCCY 3 cut(s) 34, 793, 2015
PscI ACATGT 1 cut(s) 459
PsiI TTATAA 1 cut(s) 1203
Psp124BI GAGCTC 3 cut(s) 2386, 2551, 2636
Psp5II RGGWCCY 3 cut(s) 34, 793, 2015
Psp6I CCWGG 2 cut(s) 1060, 1878
PspFI CCCAGC 2 cut(s) 588, 867
PspGI CCWGG 2 cut(s) 1060, 1878
PspN4I GGNNCC 8 cut(s) 35, 241, 449, 786, 795, 900, 2016, 2256
PspPI GGNCC 4 cut(s) 34, 793, 2015, 2699
PspPPI RGGWCCY 3 cut(s) 34, 793, 2015
PspXI VCTCGAGB 1 cut(s) 1983
PstI CTGCAG 3 cut(s) 1043, 1133, 2023
PstNI CAGNNNCTG 1 cut(s) 2409
PsuI RGATCY 3 cut(s) 1102, 2254, 2483
PvuII CAGCTG 2 cut(s) 659, 1226
RsaI GTAC 4 cut(s) 1157, 1174, 1256, 2084
RsaNI GTAC 4 cut(s) 1156, 1173, 1255, 2083
RseI CAYNNNNRTG 6 cut(s) 420, 675, 831, 1121, 1815, 2174
SacI GAGCTC 3 cut(s) 2386, 2551, 2636
SapI GCTCTTC 1 cut(s) 2624
SaqAI TTAA 7 cut(s) 39, 179, 185, 375, 1712, 1944, 2743
SatI GCNGC 9 cut(s) 334, 657, 930, 1042, 1132, 1884, 2393, 2404, 2732
Sau96I GGNCC 4 cut(s) 34, 793, 2015, 2699
ScaI AGTACT 1 cut(s) 2084
SchI GAGTC 3 cut(s) 1571, 1872, 2053
ScrFI CCNGG 2 cut(s) 1062, 1880
SduI GDGCHC 5 cut(s) 1060, 1510, 2386, 2551, 2636
SfaNI GCATC 5 cut(s) 370, 510, 2509, 2602, 2608
SfcI CTRYAG 5 cut(s) 1039, 1129, 2019, 2086, 2367
Sfr274I CTCGAG 1 cut(s) 1983
SfuI TTCGAA 2 cut(s) 1673, 1858
SinI GGWCC 3 cut(s) 34, 793, 2015
SlaI CTCGAG 1 cut(s) 1983
SmiMI CAYNNNNRTG 6 cut(s) 420, 675, 831, 1121, 1815, 2174
SmlI CTYRAG 3 cut(s) 1834, 1983, 2712
SmoI CTYRAG 3 cut(s) 1834, 1983, 2712
SnaBI TACGTA 1 cut(s) 1414
SpeI ACTAGT 1 cut(s) 602
SphI GCATGC 1 cut(s) 1126
SsiI CCGC 4 cut(s) 630, 1375, 1504, 2054
SspMI CTAG 7 cut(s) 119, 603, 702, 1694, 1887, 2063, 2646
SstI GAGCTC 3 cut(s) 2386, 2551, 2636
StyD4I CCNGG 2 cut(s) 1060, 1878
StyI CCWWGG 4 cut(s) 853, 903, 1816, 2637
TaaI ACNGT 8 cut(s) 152, 173, 580, 1194, 1208, 1244, 1394, 1624
TaiI ACGT 2 cut(s) 1390, 1416
TaqI TCGA 6 cut(s) 471, 1673, 1858, 1936, 1965, 1984
TaqII GACCGA 1 cut(s) 2593
TatI WGTACW 2 cut(s) 1172, 2082
TfiI GAWTC 5 cut(s) 308, 837, 1471, 2149, 2189
Tru1I TTAA 7 cut(s) 39, 179, 185, 375, 1712, 1944, 2743
Tru9I TTAA 7 cut(s) 39, 179, 185, 375, 1712, 1944, 2743
TseFI GTSAC 4 cut(s) 1388, 1864, 2004, 2362
TseI GCWGC 9 cut(s) 333, 656, 929, 1041, 1131, 1883, 2392, 2403, 2731
Tsp45I GTSAC 4 cut(s) 1388, 1864, 2004, 2362
TspDTI ATGAA 8 cut(s) 815, 896, 1086, 1239, 1290, 1774, 2199, 2544
TspGWI ACGGA 1 cut(s) 124
Van91I CCANNNNNTGG 1 cut(s) 263
VpaK11BI GGWCC 3 cut(s) 34, 793, 2015
XagI CCTNNNNNAGG 1 cut(s) 441
XapI RAATTY 5 cut(s) 66, 94, 545, 1669, 2540
XbaI TCTAGA 1 cut(s) 701
XceI RCATGY 3 cut(s) 463, 1126, 1663
XcmI CCANNNNNNNNNTGG 2 cut(s) 1460, 1814
XhoI CTCGAG 1 cut(s) 1983
XmnI GAANNNNTTC 2 cut(s) 1679, 2723
XspI CTAG 7 cut(s) 119, 603, 702, 1694, 1887, 2063, 2646
ZrmI AGTACT 1 cut(s) 2084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.