Rmu_co8285195.1_g000001

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8285195.1
OG0000716
Physical Location & Seq
Reverse (-)
2 .. 858
857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8285195.1_g000001.1.cds

Sequence Viewer

Length: 857 bp
ctcactcgcaaactctaatgcgtgtacaacagagtgcgattcttcccccaagccaggctgcaacaacaacacatgtagtgtcctcccagacaactccgtgatccctacgtccggcaatggcggggaggtaggtcaagacgtcgtctccctccattccacaaatgggtctaaccctaccacactagtttatgttcctaacctcctcttcgcttgtgctgaaatttttctattggatcgccttgcaagtggtgtcaaaggtatggctggtcttggtagagctaaaattggacttccttatctatttgcttcctcttttagcttcaaaagaaaattcaccattttcttgccctcctcaacaaaatcatacgatgccgttttcttcggagatgggccttacaacctttttcttgggattgacatctctcaatctcttatttacacctcattgctcctcaaccctgtaagcactgccagtgcctattttcaaggcgatgcttctacggaatacttcatcaatgtaacgtcaatcaagatcaacgacaaggtcgtttcgtcatttaacagttccttgctaaccattgacggaaatggttatggcggcacgaagatcaacacggtgcatccctacacggtcttggagatgtcaatctacaactctttggtcaatgcatttgtcagtgaactctctcatgtacccagagtgacactggtagcaccattcggagcttgcttcaactcaaccaacattgtcagcacgagtgttggcctggctatgccaaccatcgaccttgtgttgcagagtgagagcgtgtactggagaatctttggtgcaaactcgatggtgcaggtgaccaagg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

285

Amino Acids

30.35

Weight (kDa)

5.55

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 836
AatII GACGTC 1 cut(s) 142
Acc36I ACCTGC 1 cut(s) 836
AciI CCGC 2 cut(s) 121, 598
AclWI GGATC 2 cut(s) 95, 241
AcsI RAATTY 2 cut(s) 220, 330
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 3 cut(s) 26, 694, 813
AfiI CCNNNNNNNGG 3 cut(s) 54, 111, 163
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 3 cut(s) 323, 486, 734
AhlI ACTAGT 1 cut(s) 182
AjnI CCWGG 2 cut(s) 53, 766
AluBI AGCT 3 cut(s) 279, 319, 726
AluI AGCT 3 cut(s) 279, 319, 726
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 2 cut(s) 95, 241
AoxI GGCC 2 cut(s) 390, 764
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 2 cut(s) 220, 330
Asp700I GAANNNNTTC 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 390
AsuHPI GGTGA 1 cut(s) 326
BauI CACGAG 1 cut(s) 755
BbvI GCAGC 1 cut(s) 45
BccI CCATC 3 cut(s) 381, 789, 833
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 2 cut(s) 55, 768
BcoDI GTCTC 1 cut(s) 149
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 836
BisI GCNGC 2 cut(s) 59, 599
BlsI GCNGC 2 cut(s) 60, 600
Bme1390I CCNGG 2 cut(s) 55, 768
BmgT120I GGNCC 1 cut(s) 390
BmrFI CCNGG 2 cut(s) 55, 768
BmsI GCATC 3 cut(s) 359, 482, 629
BpmI CTGGAG 1 cut(s) 836
BsaBI GATNNNNATC 2 cut(s) 417, 645
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 853
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 111, 163
Bse1I ACTGG 3 cut(s) 472, 712, 819
Bse3DI GCAATG 2 cut(s) 122, 444
Bse8I GATNNNNATC 2 cut(s) 417, 645
BseBI CCWGG 2 cut(s) 55, 768
BseDI CCNNGG 1 cut(s) 853
BseGI GGATG 1 cut(s) 620
BseJI GATNNNNATC 2 cut(s) 417, 645
BseLI CCNNNNNNNGG 3 cut(s) 54, 111, 163
BseMI GCAATG 2 cut(s) 122, 444
BseNI ACTGG 3 cut(s) 472, 712, 819
BseRI GAGGAG 3 cut(s) 192, 341, 441
BseXI GCAGC 1 cut(s) 45
BshFI GGCC 2 cut(s) 392, 766
BsiSI CCGG 1 cut(s) 112
BslI CCNNNNNNNGG 3 cut(s) 54, 111, 163
BsmAI GTCTC 1 cut(s) 149
BsmBI CGTCTC 1 cut(s) 149
BsnI GGCC 2 cut(s) 392, 766
Bsp1407I TGTACA 1 cut(s) 24
Bsp143I GATC 4 cut(s) 100, 233, 532, 607
BspACI CCGC 2 cut(s) 121, 598
BspANI GGCC 2 cut(s) 392, 766
BspMI ACCTGC 1 cut(s) 836
BspPI GGATC 2 cut(s) 95, 241
BsrDI GCAATG 2 cut(s) 122, 444
BsrGI TGTACA 1 cut(s) 24
BsrI ACTGG 3 cut(s) 472, 712, 819
BssECI CCNNGG 1 cut(s) 853
BssMI GATC 4 cut(s) 100, 233, 532, 607
BssNI GRCGYC 1 cut(s) 139
BssSI CACGAG 1 cut(s) 755
BssT1I CCWWGG 1 cut(s) 853
Bst2BI CACGAG 1 cut(s) 755
Bst2UI CCWGG 2 cut(s) 55, 768
Bst4CI ACNGT 3 cut(s) 564, 617, 632
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 139
BstAUI TGTACA 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 728
BstEII GGTNACC 1 cut(s) 848
BstF5I GGATG 1 cut(s) 620
BstKTI GATC 4 cut(s) 103, 236, 535, 610
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 4 cut(s) 100, 233, 532, 607
BstNI CCWGG 2 cut(s) 55, 768
BstNSI RCATGY 1 cut(s) 76
BstPI GGTNACC 1 cut(s) 848
BstSCI CCNGG 2 cut(s) 53, 766
BstV1I GCAGC 1 cut(s) 45
BsuRI GGCC 2 cut(s) 392, 766
BtgZI GCGATG 1 cut(s) 505
BtsCI GGATG 1 cut(s) 620
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 4 cut(s) 466, 479, 683, 705
BveI ACCTGC 1 cut(s) 836
Cac8I GCNNGC 1 cut(s) 728
Cfr13I GGNCC 1 cut(s) 390
Csp6I GTAC 3 cut(s) 25, 693, 812
CviAII CATG 2 cut(s) 73, 690
CviJI RGCY 9 cut(s) 53, 58, 264, 279, 319, 392, 726, 766, 771
CviKI_1 RGCY 9 cut(s) 53, 58, 264, 279, 319, 392, 726, 766, 771
CviQI GTAC 3 cut(s) 25, 693, 812
DpnI GATC 4 cut(s) 102, 235, 534, 609
DpnII GATC 4 cut(s) 100, 233, 532, 607
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 853
Eco91I GGTNACC 1 cut(s) 848
EcoO65I GGTNACC 1 cut(s) 848
EcoRII CCWGG 2 cut(s) 53, 766
EcoT14I CCWWGG 1 cut(s) 853
EcoT22I ATGCAT 1 cut(s) 671
ErhI CCWWGG 1 cut(s) 853
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 2 cut(s) 76, 693
FaiI YATR 7 cut(s) 74, 190, 261, 365, 595, 691, 774
FatI CATG 2 cut(s) 72, 689
FauI CCCGC 1 cut(s) 114
Fnu4HI GCNGC 2 cut(s) 59, 599
FokI GGATG 1 cut(s) 607
Fsp4HI GCNGC 2 cut(s) 59, 599
FspBI CTAG 1 cut(s) 183
GluI GCNGC 2 cut(s) 59, 599
GsuI CTGGAG 1 cut(s) 836
HaeIII GGCC 2 cut(s) 392, 766
HapII CCGG 1 cut(s) 112
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 2 cut(s) 76, 693
HinfI GANTC 2 cut(s) 39, 820
HpaII CCGG 1 cut(s) 112
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 3 cut(s) 25, 681, 812
Hpy188I TCNGA 2 cut(s) 384, 723
Hpy188III TCNNGA 2 cut(s) 135, 530
Hpy8I GTNNAC 3 cut(s) 25, 681, 812
Hpy99I CGWCG 1 cut(s) 144
HpyCH4III ACNGT 3 cut(s) 564, 617, 632
HpyCH4IV ACGT 3 cut(s) 108, 139, 522
HpyCH4V TGCA 7 cut(s) 61, 243, 620, 669, 797, 831, 845
HpySE526I ACGT 3 cut(s) 108, 139, 522
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 2 cut(s) 76, 693
Kzo9I GATC 4 cut(s) 100, 233, 532, 607
LmnI GCTCC 2 cut(s) 454, 723
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 3 cut(s) 359, 482, 629
MaeI CTAG 1 cut(s) 183
MaeII ACGT 3 cut(s) 108, 139, 522
MaeIII GTNAC 3 cut(s) 518, 701, 848
MalI GATC 4 cut(s) 102, 235, 534, 609
MboI GATC 4 cut(s) 100, 233, 532, 607
MboII GAAGA 4 cut(s) 34, 197, 371, 617
MluCI AATT 3 cut(s) 220, 283, 330
Mph1103I ATGCAT 1 cut(s) 671
MroXI GAANNNNTTC 1 cut(s) 223
MseI TTAA 1 cut(s) 559
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 2 cut(s) 55, 768
MvaI CCWGG 2 cut(s) 55, 768
NdeII GATC 4 cut(s) 100, 233, 532, 607
NlaIII CATG 2 cut(s) 76, 693
NmuCI GTSAC 2 cut(s) 701, 848
NsiI ATGCAT 1 cut(s) 671
NspI RCATGY 1 cut(s) 76
PaqCI CACCTGC 1 cut(s) 836
PciI ACATGT 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 544
PdmI GAANNNNTTC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 39, 820
PflFI GACNNNGTC 2 cut(s) 141, 543
PkrI GCNGC 2 cut(s) 60, 600
PscI ACATGT 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 53, 766
PspEI GGTNACC 1 cut(s) 848
PspGI CCWGG 2 cut(s) 53, 766
PspPI GGNCC 1 cut(s) 390
PsyI GACNNNGTC 2 cut(s) 141, 543
RsaI GTAC 3 cut(s) 26, 694, 813
RsaNI GTAC 3 cut(s) 25, 693, 812
SaqAI TTAA 1 cut(s) 559
SatI GCNGC 2 cut(s) 59, 599
Sau3AI GATC 4 cut(s) 100, 233, 532, 607
Sau96I GGNCC 1 cut(s) 390
ScrFI CCNGG 2 cut(s) 55, 768
SfaNI GCATC 3 cut(s) 359, 482, 629
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 3 cut(s) 220, 283, 330
SsiI CCGC 2 cut(s) 121, 598
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 53, 766
StyI CCWWGG 1 cut(s) 853
TaaI ACNGT 3 cut(s) 564, 617, 632
TaiI ACGT 3 cut(s) 111, 142, 525
TaqI TCGA 2 cut(s) 784, 837
TasI AATT 3 cut(s) 220, 283, 330
TatI WGTACW 2 cut(s) 24, 811
TauI GCSGC 1 cut(s) 601
TfiI GAWTC 2 cut(s) 39, 820
Tru1I TTAA 1 cut(s) 559
Tru9I TTAA 1 cut(s) 559
TscAI CASTG 4 cut(s) 473, 479, 683, 712
TseFI GTSAC 2 cut(s) 701, 848
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 2 cut(s) 701, 848
TspDTI ATGAA 1 cut(s) 500
TspGWI ACGGA 3 cut(s) 86, 516, 598
TspRI CASTG 4 cut(s) 473, 479, 683, 712
Tth111I GACNNNGTC 2 cut(s) 141, 543
XapI RAATTY 2 cut(s) 220, 330
XceI RCATGY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 2 cut(s) 159, 704
XmnI GAANNNNTTC 1 cut(s) 223
XspI CTAG 1 cut(s) 183
ZraI GACGTC 1 cut(s) 140
Zsp2I ATGCAT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.