MD16G1185100.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000716
Physical Location & Seq
Forward (+)
15947253 .. 15947879
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1185100.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCTTTCTCCTCATTTCATTGCCTTTTCTTTTGCTCATTACTCTTCCACTTCATCGTCGCCCCTTCACTTGCCAAAACTGCTTCTTTCAGACCAAAAGCCCTTGTTCTTCCTGTGGTAAAAGACTCCTCTACCCTCCAATACCTCACCACCTTCAGCCAAAGAACCCCTTTTGTGGACTGTGAAAAGGACTACATTTCCTCCACCTACAAGCCGGCTCGCTGCAACTCCGCCCAATGCTCACTCTCAAACTCCAAGTCATGTACTACAGAGTGCTACTCTTCCCCTAAGCCAGGCTGCAACAACAATACATGTGCTCTCTCCCCCGGTAACACCATCACCCACACCACCACAAGCAGCGACCTTGGTCAAGACACTGTGTCCCTACAATCCACAGATGGGTCTAATCCAGGTAGGCTGGTCTCTGTCCCCAACCTACTCTTCATTTATGGGCCCATATTTCTGTTAGAAGGCCTAGCGAGTGGTGTCAAGGGCATGGCTGGCCTCGGTAGGGATAAAATCGGGCTTCTATCTCAATTCGCTTCTGCCTTTAGCTTCCCCCGAAAGTTTGCAATTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

194

Amino Acids

20.77

Weight (kDa)

8.89

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 62 - 192 2.2e-26 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 231
AcuI CTGAAG 1 cut(s) 139
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 4 cut(s) 175, 293, 399, 511
AflIII ACRYGT 1 cut(s) 311
AhdI GACNNNNNGTC 1 cut(s) 379
AjnI CCWGG 2 cut(s) 292, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AluBI AGCT 1 cut(s) 555
AluI AGCT 1 cut(s) 555
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 427
AoxI GGCC 3 cut(s) 452, 472, 502
ApaI GGGCCC 1 cut(s) 456
ApeKI GCWGC 3 cut(s) 222, 297, 357
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspS9I GGNCC 2 cut(s) 452, 453
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 139, 331
BaeGI GKGCMC 1 cut(s) 456
BanII GRGCYC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 3 cut(s) 209, 284, 369
BccI CCATC 2 cut(s) 344, 392
BciT130I CCWGG 2 cut(s) 294, 411
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 427
BfaI CTAG 1 cut(s) 476
BfmI CTRYAG 1 cut(s) 267
BisI GCNGC 3 cut(s) 223, 298, 358
BlsI GCNGC 3 cut(s) 224, 299, 359
Bme1390I CCNGG 3 cut(s) 294, 327, 411
BmeRI GACNNNNNGTC 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 452, 453
BmiI GGNNCC 1 cut(s) 454
BmrFI CCNGG 3 cut(s) 294, 327, 411
BoxI GACNNNNGTC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 263, 295
Bpu10I CCTNAGC 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 427
BsaJI CCNNGG 3 cut(s) 325, 364, 505
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 293, 399, 511
Bse118I RCCGGY 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 19
BseBI CCWGG 2 cut(s) 294, 411
BseDI CCNNGG 3 cut(s) 325, 364, 505
BseLI CCNNNNNNNGG 4 cut(s) 175, 293, 399, 511
BseMI GCAATG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 118
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 3 cut(s) 209, 284, 369
BshFI GGCC 3 cut(s) 454, 474, 504
BsiHKAI GWGCWC 1 cut(s) 319
BsiSI CCGG 2 cut(s) 215, 327
BslFI GGGAC 2 cut(s) 367, 413
BslI CCNNNNNNNGG 4 cut(s) 175, 293, 399, 511
BsmAI GTCTC 1 cut(s) 427
BsmFI GGGAC 2 cut(s) 367, 413
BsnI GGCC 3 cut(s) 454, 474, 504
Bso31I GGTCTC 1 cut(s) 427
Bsp120I GGGCCC 1 cut(s) 452
Bsp1286I GDGCHC 2 cut(s) 319, 456
BspACI CCGC 1 cut(s) 231
BspANI GGCC 3 cut(s) 454, 474, 504
BspLI GGNNCC 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 3 cut(s) 325, 364, 505
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 2 cut(s) 294, 411
Bst4CI ACNGT 2 cut(s) 182, 379
Bst6I CTCTTC 3 cut(s) 50, 286, 446
BstC8I GCNNGC 3 cut(s) 216, 220, 502
BstDEI CTNAG 1 cut(s) 288
BstENI CCTNNNNNAGG 1 cut(s) 291
BstMAI GTCTC 1 cut(s) 427
BstMWI GCNNNNNNNGC 2 cut(s) 80, 501
BstNI CCWGG 2 cut(s) 294, 411
BstNSI RCATGY 1 cut(s) 315
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 3 cut(s) 292, 325, 409
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 209, 284, 369
BsuRI GGCC 3 cut(s) 454, 474, 504
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 216, 220, 502
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 452, 453
Csp6I GTAC 1 cut(s) 264
CviAII CATG 3 cut(s) 261, 312, 496
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 1 cut(s) 288
DriI GACNNNNNGTC 1 cut(s) 379
Eam1104I CTCTTC 3 cut(s) 50, 286, 446
Eam1105I GACNNNNNGTC 1 cut(s) 379
EarI CTCTTC 3 cut(s) 50, 286, 446
EciI GGCGGA 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 364
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 139
EcoNI CCTNNNNNAGG 1 cut(s) 291
EcoRII CCWGG 2 cut(s) 292, 409
EcoT14I CCWWGG 1 cut(s) 364
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 3 cut(s) 264, 315, 499
FaiI YATR 5 cut(s) 262, 313, 450, 458, 497
FalI AAGNNNNNCTT 2 cut(s) 154, 186
FaqI GGGAC 2 cut(s) 367, 413
FatI CATG 3 cut(s) 260, 311, 495
Fnu4HI GCNGC 3 cut(s) 223, 298, 358
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 3 cut(s) 223, 298, 358
FspBI CTAG 1 cut(s) 476
GluI GCNGC 3 cut(s) 223, 298, 358
HaeIII GGCC 3 cut(s) 454, 474, 504
HapII CCGG 2 cut(s) 215, 327
Hin1II CATG 3 cut(s) 264, 315, 499
HinfI GANTC 1 cut(s) 125
HpaII CCGG 2 cut(s) 215, 327
HphI GGTGA 2 cut(s) 139, 331
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 1 cut(s) 92
Hpy188III TCNNGA 1 cut(s) 371
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 75, 163, 464
HpyCH4III ACNGT 2 cut(s) 182, 379
HpyCH4V TGCA 3 cut(s) 225, 300, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 501
HpyF3I CTNAG 1 cut(s) 288
Hsp92II CATG 3 cut(s) 264, 315, 499
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
LpnPI CCDG 9 cut(s) 126, 228, 279, 306, 340, 396, 404, 423, 486
Lsp1109I GCAGC 3 cut(s) 209, 284, 369
MaeI CTAG 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 329
MboII GAAGA 4 cut(s) 37, 101, 273, 433
MhlI GDGCHC 2 cut(s) 319, 456
MluCI AATT 2 cut(s) 536, 573
MlyI GAGTC 1 cut(s) 119
MnlI CCTC 6 cut(s) 22, 139, 146, 155, 211, 515
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 1 cut(s) 580
MspI CCGG 2 cut(s) 215, 327
MspR9I CCNGG 3 cut(s) 294, 327, 411
MvaI CCWGG 2 cut(s) 294, 411
MwoI GCNNNNNNNGC 2 cut(s) 80, 501
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 327
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 3 cut(s) 264, 315, 499
NlaIV GGNNCC 1 cut(s) 454
NspI RCATGY 1 cut(s) 315
PceI AGGCCT 1 cut(s) 474
PciI ACATGT 1 cut(s) 311
PdiI GCCGGC 1 cut(s) 216
PkrI GCNGC 3 cut(s) 224, 299, 359
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PscI ACATGT 1 cut(s) 311
PshAI GACNNNNGTC 1 cut(s) 366
Psp6I CCWGG 2 cut(s) 292, 409
PspGI CCWGG 2 cut(s) 292, 409
PspN4I GGNNCC 1 cut(s) 454
PspOMI GGGCCC 1 cut(s) 452
PspPI GGNCC 2 cut(s) 452, 453
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SaqAI TTAA 1 cut(s) 580
SatI GCNGC 3 cut(s) 223, 298, 358
Sau96I GGNCC 2 cut(s) 452, 453
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 3 cut(s) 294, 327, 411
SduI GDGCHC 2 cut(s) 319, 456
SetI ASST 7 cut(s) 147, 155, 209, 366, 415, 438, 557
SfcI CTRYAG 1 cut(s) 267
Sse9I AATT 2 cut(s) 536, 573
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 1 cut(s) 231
SspMI CTAG 1 cut(s) 476
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 3 cut(s) 292, 325, 409
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 2 cut(s) 182, 379
TasI AATT 2 cut(s) 536, 573
TatI WGTACW 1 cut(s) 263
Tru1I TTAA 1 cut(s) 580
Tru9I TTAA 1 cut(s) 580
TscAI CASTG 1 cut(s) 382
TseI GCWGC 3 cut(s) 222, 297, 357
TspDTI ATGAA 3 cut(s) 8, 43, 433
TspRI CASTG 1 cut(s) 382
XagI CCTNNNNNAGG 1 cut(s) 291
XceI RCATGY 1 cut(s) 315
XspI CTAG 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.