MD15G1028800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000947
Physical Location & Seq
Reverse (-)
1764019 .. 1765906
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1028800.v1.1.491

Sequence Viewer

Length: 465 bp
ATGGAAAGGTCAGCGGCGATTTCGAGTGCTTCGATGCAGAAAGGATCGACAACGAGAGATTTGATCGGAGGTTGTAAGTTTTTCAGGACAAATGGCAGAGACTTTAGGGTTTCTTGGACGACGAGAAAGATGCGAACTGAGATACCGATGGGTTGTTGGGACACGTCGACGCGGGGGAGGCTGACGACATGGAGGTCGGAAGGGAGAGAGGGGTTGCTGAGGAGCTGGAGCTGTGCGGGTGGTGCGTCGGTGGTGATGGAAAGGAAGGTGACATGGAAGCCATGGTCAACGACGAGTCGTTTGGCTAGCTCGAGGAGTGGGGTAACGTGGCCAATCCCTGGGCTTGCTAGGACTGCCACATGCGGCTTCTTCTGGCCGGACTCCATGTATGGCAAGAAAAAAGAGCCAAAGATATACAGTGGATTTGAGAGACAGAGACAATCTTTAACTCTGAAGAAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.48

Weight (kDa)

10.93

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 193
AccB7I CCANNNNNTGG 1 cut(s) 338
AccI GTMKAC 1 cut(s) 167
AccII CGCG 1 cut(s) 172
AciI CCGC 4 cut(s) 14, 172, 236, 363
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 2 cut(s) 329, 374
AfiI CCNNNNNNNGG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 162
AjiI CACGTC 1 cut(s) 165
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 3 cut(s) 225, 231, 309
AluI AGCT 3 cut(s) 225, 231, 309
Alw26I GTCTC 3 cut(s) 93, 424, 430
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 310
AoxI GGCC 2 cut(s) 329, 374
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
AsuHPI GGTGA 2 cut(s) 265, 280
AsuNHI GCTAGC 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 310
BalI TGGCCA 1 cut(s) 331
BbvCI CCTCAGC 1 cut(s) 218
BccI CCATC 2 cut(s) 142, 250
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciT130I CCWGG 1 cut(s) 339
BcoDI GTCTC 3 cut(s) 93, 424, 430
BfaI CTAG 2 cut(s) 306, 348
BisI GCNGC 2 cut(s) 15, 364
BlsI GCNGC 2 cut(s) 16, 365
Bme1390I CCNGG 1 cut(s) 339
BmeT110I CYCGRG 1 cut(s) 310
BmgBI CACGTC 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 24, 120
BmtI GCTAGC 1 cut(s) 309
BpmI CTGGAG 1 cut(s) 247
Bpu10I CCTNAGC 1 cut(s) 218
BsaJI CCNNGG 3 cut(s) 281, 337, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 338
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 3 cut(s) 281, 337, 338
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 2 cut(s) 129, 209
BseRI GAGGAG 2 cut(s) 235, 328
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 2 cut(s) 331, 376
BsiHKCI CYCGRG 1 cut(s) 310
BsiSI CCGG 1 cut(s) 377
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 3 cut(s) 93, 424, 430
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 2 cut(s) 331, 376
BsoBI CYCGRG 1 cut(s) 310
Bsp143I GATC 2 cut(s) 44, 63
Bsp19I CCATGG 1 cut(s) 281
BspACI CCGC 4 cut(s) 14, 172, 236, 363
BspANI GGCC 2 cut(s) 331, 376
BspCNI CTCAG 2 cut(s) 130, 210
BspFNI CGCG 1 cut(s) 172
BspOI GCTAGC 1 cut(s) 309
BspPI GGATC 1 cut(s) 52
BssECI CCNNGG 3 cut(s) 281, 337, 338
BssMI GATC 2 cut(s) 44, 63
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 2 cut(s) 419, 462
BstC8I GCNNGC 2 cut(s) 307, 345
BstDEI CTNAG 2 cut(s) 138, 218
BstDSI CCRYGG 1 cut(s) 281
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 2 cut(s) 47, 66
BstMAI GTCTC 3 cut(s) 93, 424, 430
BstMBI GATC 2 cut(s) 44, 63
BstMWI GCNNNNNNNGC 3 cut(s) 178, 242, 353
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 337
BstUI CGCG 1 cut(s) 172
BsuRI GGCC 2 cut(s) 331, 376
BtgI CCRYGG 1 cut(s) 281
BtrI CACGTC 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 424
Cac8I GCNNGC 2 cut(s) 307, 345
CseI GACGC 2 cut(s) 178, 234
CviAII CATG 5 cut(s) 189, 273, 282, 360, 385
DdeI CTNAG 2 cut(s) 138, 218
DpnI GATC 2 cut(s) 46, 65
DpnII GATC 2 cut(s) 44, 63
DrdI GACNNNNNNGTC 1 cut(s) 193
DseDI GACNNNNNNGTC 1 cut(s) 193
EaeI YGGCCR 2 cut(s) 329, 374
Eco130I CCWWGG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 5 cut(s) 192, 276, 285, 363, 388
FaiI YATR 7 cut(s) 190, 274, 283, 361, 386, 390, 415
FaqI GGGAC 1 cut(s) 173
FatI CATG 5 cut(s) 188, 272, 281, 359, 384
FauI CCCGC 2 cut(s) 165, 229
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 2 cut(s) 15, 364
Fsp4HI GCNGC 2 cut(s) 15, 364
FspBI CTAG 2 cut(s) 306, 348
GluI GCNGC 2 cut(s) 15, 364
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 2 cut(s) 331, 376
HapII CCGG 1 cut(s) 377
HgaI GACGC 2 cut(s) 178, 234
Hin1II CATG 5 cut(s) 192, 276, 285, 363, 388
HincII GTYRAC 2 cut(s) 168, 288
HindII GTYRAC 2 cut(s) 168, 288
HinfI GANTC 2 cut(s) 295, 380
HpaII CCGG 1 cut(s) 377
HphI GGTGA 2 cut(s) 265, 280
Hpy166II GTNNAC 2 cut(s) 168, 288
Hpy188I TCNGA 3 cut(s) 68, 199, 453
Hpy188III TCNNGA 1 cut(s) 85
Hpy8I GTNNAC 2 cut(s) 168, 288
Hpy99I CGWCG 5 cut(s) 124, 169, 172, 250, 295
HpyAV CCTTC 2 cut(s) 194, 259
HpyCH4III ACNGT 2 cut(s) 419, 462
HpyCH4IV ACGT 2 cut(s) 164, 326
HpyCH4V TGCA 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 242, 353
HpyF3I CTNAG 2 cut(s) 138, 218
HpySE526I ACGT 2 cut(s) 164, 326
Hsp92II CATG 5 cut(s) 192, 276, 285, 363, 388
Kzo9I GATC 2 cut(s) 44, 63
LmnI GCTCC 2 cut(s) 222, 228
LpnPI CCDG 6 cut(s) 70, 211, 324, 351, 358, 390
LweI GCATC 2 cut(s) 24, 120
MaeI CTAG 2 cut(s) 306, 348
MaeII ACGT 2 cut(s) 164, 326
MaeIII GTNAC 2 cut(s) 268, 322
MalI GATC 2 cut(s) 46, 65
MboI GATC 2 cut(s) 44, 63
MboII GAAGA 1 cut(s) 361
MlsI TGGCCA 1 cut(s) 331
MluNI TGGCCA 1 cut(s) 331
MlyI GAGTC 2 cut(s) 304, 374
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 6 cut(s) 62, 171, 186, 202, 213, 306
Mox20I TGGCCA 1 cut(s) 331
MscI TGGCCA 1 cut(s) 331
MseI TTAA 1 cut(s) 446
Msp20I TGGCCA 1 cut(s) 331
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 3 cut(s) 178, 242, 353
NcoI CCATGG 1 cut(s) 281
NdeII GATC 2 cut(s) 44, 63
NheI GCTAGC 1 cut(s) 305
NlaIII CATG 5 cut(s) 192, 276, 285, 363, 388
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 363
PaeR7I CTCGAG 1 cut(s) 310
PasI CCCWGGG 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 2 cut(s) 16, 365
PleI GAGTC 2 cut(s) 303, 374
PpsI GAGTC 2 cut(s) 303, 374
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspXI VCTCGAGB 1 cut(s) 310
SalI GTCGAC 1 cut(s) 166
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 2 cut(s) 15, 364
Sau3AI GATC 2 cut(s) 44, 63
SchI GAGTC 2 cut(s) 304, 374
ScrFI CCNGG 1 cut(s) 339
SetI ASST 9 cut(s) 11, 73, 167, 197, 227, 233, 270, 311, 329
SfaNI GCATC 2 cut(s) 24, 120
Sfr274I CTCGAG 1 cut(s) 310
SgrDI CGTCGACG 1 cut(s) 166
SlaI CTCGAG 1 cut(s) 310
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
SsiI CCGC 4 cut(s) 14, 172, 236, 363
SspMI CTAG 2 cut(s) 306, 348
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 2 cut(s) 419, 462
TaiI ACGT 2 cut(s) 167, 329
TaqI TCGA 5 cut(s) 23, 32, 47, 167, 311
TauI GCSGC 2 cut(s) 17, 366
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 268
Tsp45I GTSAC 1 cut(s) 268
TspRI CASTG 1 cut(s) 424
Van91I CCANNNNNTGG 1 cut(s) 338
XceI RCATGY 1 cut(s) 363
XhoI CTCGAG 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 167
XspI CTAG 2 cut(s) 306, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.