Rh2CG165100

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000947
Physical Location & Seq
Forward (+)
14769299 .. 14771043
1745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG165100.1

Sequence Viewer

Length: 1587 bp
ATGGGGAATTTATGTGAAAATTTGTGGATTGAGATATTGGGTAGGTTAACCTCGGAGAAGGACTTGGTGCCATGCATGAGTGTGTCCAAGTTATGGCACCGAATCACATCGGATCTCTGGGTGGGAAAATTTTGGGCGCAGTCCCCTCCTTTGGGTCTTTTCTTGCGCACCGCCGCCAACACCACCGTGGGCAATCTAAAATATCATTGCATGAATTATATCTCTTTATATAATTATCATGACATAGAAGATAACCTGGAAAGCACCCGCGTTTTCGTTTTCGGACAACCATATTATGACATAGAAGATTATAGGGACCAAAAGATGATTTTGCAGACTTTTGGTAGAGAATGGCATTATCCGGGTTATTATGATGATGACGAGGATGATGGTGTTCTTGATGATGATTATAAACCCGCTGATCGTGATCAGTACCTAAACGGTTGTAATGGGTTACTTTTGTTATTGAAATCAGCAACTCATCAATTTTATGTTTGCAATCCCATCACAAGACAACAAGTCGCCATACCTAAAGCCCGTGTGCACAAAAACCATGATGATCAGCATTTTTGTGCTGCCTTGGCTTTTGACCCTTCTGAATCTCCTCATCACTACAGAATTGTGAGAATTGATTTTTCTCCGGAACCGGCTTCATCAGCCTCAGACTCCTCATCCTTGATAGACATATTTTCGTCCGAATATGGACAATGGATTCGACATAGGTTGCAGCTTGATTCAAGTCTCACTGACGGGTTGAAAGAGTGCAAGTTGTGCAGGCAATTTTTTTATTTGCGGGGAAAGTTATACAGTATAACCATGTCGTGGAACCTCCTATGCATTGACCTCAAGACTGTTGAAGCTTCCGCCCTTGAACTTCCTATCCCGGTGGCGGATAGAACCGGAGCCATGGGATGTCTTGGGGTGTCAAGGGACCTTCTTTGCTATATGAAGCGTATGCCTGATAAATCAAGTCGGTTTCACAATCTGGTAGTTTGGTCTTATGAAAAAGGTGAGTGGATTCCCCGATATAGTGTAAGTTGTCATTTGTTGGGAGAAGGAATATTGGATGATCTAGGCTATGACTACTATGACACCATGGAACCCTATGCAATAAGCCCAAGTTCGGATCTCTTGTTCTTTGGCACTCCAACCTTGATTTACTGTATCCACCTTAAAAGCGAAAATATGAAATTCGTTGGTGAAACGAGCTGTAAAATAGATACACCTGCTTGTTTCTTGACATTACGTGCTTGCTTCGTACCTTTCCACACTCAATTGCGTAAGGGGAACCACGTCTCCATTTCATTAGAAATGGAAGCAATGAGTTCTGAGGGAACAAACATTTTGAGTGTAGAAGAACCAAATGGCAAACAAGAAGATGCTGAATCAGTGAAGGTAATAGATGTGAAGTTATATGATAGTATGAAAAAAGATCCATCGCCGTCATTGGTTTATCCCAGTGAAATCATGGAAATGCATCTAGAGCTTGAGCAAATCCATGACGACACCTCTAGTGATGATGAACATGTCGTAGATCAGCTTGAAGGTCTTGTAGGGAGTATGAGGAAGAGGAGGAAAATCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

528

Amino Acids

60.6

Weight (kDa)

5.16

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 140 - 295 2.4e-06 KIB1-4 beta-propeller
FBA_1 PF07734 144 - 321 1.4e-09 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 145 - 339 8.1e-11 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AarI CACCTGC 1 cut(s) 1234
Acc16I TGCGCA 1 cut(s) 167
Acc36I ACCTGC 1 cut(s) 1234
AccB1I GGYRCC 2 cut(s) 67, 96
AccB7I CCANNNNNTGG 2 cut(s) 93, 822
AccII CGCG 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 7 cut(s) 171, 174, 268, 417, 793, 864, 890
AclWI GGATC 3 cut(s) 120, 1134, 1427
AcsI RAATTY 4 cut(s) 7, 19, 128, 1190
AfaI GTAC 2 cut(s) 434, 1260
AfiI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 822, 889, 1123
AflIII ACRYGT 1 cut(s) 1525
AgsI TTSAA 6 cut(s) 469, 738, 757, 857, 872, 1544
AhdI GACNNNNNGTC 1 cut(s) 518
AjiI CACGTC 1 cut(s) 1294
AjnI CCWGG 1 cut(s) 255
AleI CACNNNNGTG 1 cut(s) 185
AloI GAACNNNNNNTCC 6 cut(s) 864, 896, 1118, 1150, 1280, 1312
AluBI AGCT 5 cut(s) 730, 860, 1209, 1486, 1540
AluI AGCT 5 cut(s) 730, 860, 1209, 1486, 1540
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 2 cut(s) 746, 1300
Alw44I GTGCAC 1 cut(s) 542
AlwI GGATC 3 cut(s) 120, 1134, 1427
Aor13HI TCCGGA 1 cut(s) 640
ApaLI GTGCAC 1 cut(s) 542
ApeKI GCWGC 2 cut(s) 575, 727
ApoI RAATTY 4 cut(s) 7, 19, 128, 1190
ArsI GACNNNNNNTTYG 2 cut(s) 696, 728
AspLEI GCGC 2 cut(s) 139, 168
AspS9I GGNCC 2 cut(s) 316, 931
AsuC2I CCSGG 2 cut(s) 363, 884
AsuHPI GGTGA 2 cut(s) 1022, 1211
AvaII GGWCC 2 cut(s) 316, 931
BaeGI GKGCMC 1 cut(s) 546
BanI GGYRCC 2 cut(s) 67, 96
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 2 cut(s) 562, 739
BccI CCATC 3 cut(s) 383, 512, 1444
BceAI ACGGC 1 cut(s) 1426
BciT130I CCWGG 1 cut(s) 257
BciVI GTATCC 1 cut(s) 1175
BclI TGATCA 2 cut(s) 427, 559
BcnI CCSGG 2 cut(s) 363, 884
BcoDI GTCTC 2 cut(s) 746, 1300
BfaI CTAG 3 cut(s) 1073, 1481, 1512
BfmI CTRYAG 1 cut(s) 613
BfuAI ACCTGC 1 cut(s) 1234
BfuI GTATCC 1 cut(s) 1175
BisI GCNGC 3 cut(s) 174, 576, 728
BlsI GCNGC 3 cut(s) 175, 577, 729
Bme1390I CCNGG 3 cut(s) 257, 363, 884
Bme18I GGWCC 2 cut(s) 316, 931
BmeRI GACNNNNNGTC 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 1294
BmgT120I GGNCC 2 cut(s) 316, 931
BmiI GGNNCC 9 cut(s) 69, 98, 317, 645, 827, 904, 932, 1101, 1289
BmrFI CCNGG 3 cut(s) 257, 363, 884
BmrI ACTGGG 1 cut(s) 1452
BmsI GCATC 2 cut(s) 1369, 1486
BmuI ACTGGG 1 cut(s) 1452
BpuEI CTTGAG 2 cut(s) 830, 1508
BpuMI CCSGG 2 cut(s) 363, 884
BsaAI YACGTR 1 cut(s) 1247
BsaBI GATNNNNATC 1 cut(s) 426
BsaJI CCNNGG 5 cut(s) 51, 186, 579, 906, 1095
BsaWI WCCGGW 2 cut(s) 640, 899
BsaXI ACNNNNNCTCC 2 cut(s) 1280, 1310
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 151, 152, 822, 889, 1123
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 2 cut(s) 1458, 1582
Bse3DI GCAATG 2 cut(s) 205, 1326
Bse8I GATNNNNATC 1 cut(s) 426
BseAI TCCGGA 1 cut(s) 640
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 5 cut(s) 51, 186, 579, 906, 1095
BseGI GGATG 4 cut(s) 391, 671, 917, 1072
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 822, 889, 1123
BseMI GCAATG 2 cut(s) 205, 1326
BseMII CTCAG 2 cut(s) 675, 1320
BseNI ACTGG 2 cut(s) 1458, 1582
BseRI GAGGAG 3 cut(s) 594, 658, 1585
BseSI GKGCMC 1 cut(s) 546
BseXI GCAGC 2 cut(s) 562, 739
BsgI GTGCAG 1 cut(s) 793
Bsh1236I CGCG 1 cut(s) 270
BshNI GGYRCC 2 cut(s) 67, 96
BsiHKAI GWGCWC 1 cut(s) 546
BsiSI CCGG 5 cut(s) 362, 641, 647, 884, 900
BslFI GGGAC 3 cut(s) 127, 329, 944
BslI CCNNNNNNNGG 6 cut(s) 93, 151, 152, 822, 889, 1123
BsmAI GTCTC 2 cut(s) 746, 1300
BsmBI CGTCTC 1 cut(s) 1300
BsmFI GGGAC 3 cut(s) 127, 329, 944
Bsp1286I GDGCHC 1 cut(s) 546
Bsp13I TCCGGA 1 cut(s) 640
Bsp143I GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1432, 1534
Bsp19I CCATGG 2 cut(s) 906, 1095
BspACI CCGC 7 cut(s) 171, 174, 268, 417, 793, 864, 890
BspCNI CTCAG 2 cut(s) 674, 1321
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 1 cut(s) 270
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 9 cut(s) 69, 98, 317, 645, 827, 904, 932, 1101, 1289
BspMI ACCTGC 1 cut(s) 1234
BspPI GGATC 3 cut(s) 120, 1134, 1427
BspT107I GGYRCC 2 cut(s) 67, 96
BsrDI GCAATG 2 cut(s) 205, 1326
BsrFI RCCGGY 1 cut(s) 646
BsrI ACTGG 2 cut(s) 1458, 1582
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 5 cut(s) 51, 186, 579, 906, 1095
BssMI GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1432, 1534
BssT1I CCWWGG 3 cut(s) 579, 906, 1095
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 5 cut(s) 187, 443, 809, 853, 1163
Bst6I CTCTTC 1 cut(s) 1562
BstAPI GCANNNNNTGC 1 cut(s) 771
BstBAI YACGTR 1 cut(s) 1247
BstC8I GCNNGC 2 cut(s) 776, 1252
BstDEI CTNAG 2 cut(s) 661, 1329
BstDSI CCRYGG 3 cut(s) 186, 906, 1095
BstF5I GGATG 4 cut(s) 391, 671, 917, 1072
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 2 cut(s) 139, 168
BstKTI GATC 8 cut(s) 115, 424, 430, 562, 1072, 1129, 1435, 1537
BstMAI GTCTC 2 cut(s) 746, 1300
BstMBI GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1432, 1534
BstMWI GCNNNNNNNGC 4 cut(s) 581, 656, 771, 1483
BstNI CCWGG 1 cut(s) 257
BstNSI RCATGY 1 cut(s) 1529
BstSCI CCNGG 3 cut(s) 255, 361, 882
BstSFI CTRYAG 1 cut(s) 613
BstSLI GKGCMC 1 cut(s) 546
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 562, 739
BstX2I RGATCY 3 cut(s) 112, 1126, 1432
BstYI RGATCY 3 cut(s) 112, 1126, 1432
BsuI GTATCC 1 cut(s) 1175
BtgI CCRYGG 3 cut(s) 186, 906, 1095
BtgZI GCGATG 1 cut(s) 1422
BtrI CACGTC 1 cut(s) 1294
BtsCI GGATG 4 cut(s) 391, 671, 917, 1072
BtsIMutI CAGTG 3 cut(s) 744, 1395, 1465
BveI ACCTGC 1 cut(s) 1234
Cac8I GCNNGC 2 cut(s) 776, 1252
CciI TCATGA 1 cut(s) 238
CfoI GCGC 2 cut(s) 139, 168
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 2 cut(s) 316, 931
Csp6I GTAC 2 cut(s) 433, 1259
CviQI GTAC 2 cut(s) 433, 1259
DdeI CTNAG 2 cut(s) 661, 1329
DpnI GATC 8 cut(s) 114, 423, 429, 561, 1071, 1128, 1434, 1536
DpnII GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1432, 1534
DriI GACNNNNNGTC 1 cut(s) 518
Eam1104I CTCTTC 1 cut(s) 1562
Eam1105I GACNNNNNGTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 1562
EciI GGCGGA 2 cut(s) 853, 905
Eco130I CCWWGG 3 cut(s) 579, 906, 1095
Eco47I GGWCC 2 cut(s) 316, 931
EcoO109I RGGNCCY 1 cut(s) 931
EcoRII CCWGG 1 cut(s) 255
EcoT14I CCWWGG 3 cut(s) 579, 906, 1095
EcoT22I ATGCAT 3 cut(s) 77, 839, 1479
ErhI CCWWGG 3 cut(s) 579, 906, 1095
Esp3I CGTCTC 1 cut(s) 1300
FaqI GGGAC 3 cut(s) 127, 329, 944
FauI CCCGC 3 cut(s) 275, 424, 786
FbaI TGATCA 2 cut(s) 427, 559
Fnu4HI GCNGC 3 cut(s) 174, 576, 728
FokI GGATG 4 cut(s) 398, 658, 924, 1079
Fsp4HI GCNGC 3 cut(s) 174, 576, 728
FspBI CTAG 3 cut(s) 1073, 1481, 1512
FspI TGCGCA 1 cut(s) 167
GlaI GCGC 2 cut(s) 138, 167
GluI GCNGC 3 cut(s) 174, 576, 728
HapII CCGG 5 cut(s) 362, 641, 647, 884, 900
HhaI GCGC 2 cut(s) 139, 168
Hin6I GCGC 2 cut(s) 137, 166
HinP1I GCGC 2 cut(s) 137, 166
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HindIII AAGCTT 1 cut(s) 858
HinfI GANTC 7 cut(s) 102, 599, 665, 712, 734, 1018, 1385
HpaI GTTAAC 1 cut(s) 48
HpaII CCGG 5 cut(s) 362, 641, 647, 884, 900
HphI GGTGA 2 cut(s) 1022, 1211
Hpy166II GTNNAC 2 cut(s) 48, 544
Hpy188I TCNGA 8 cut(s) 55, 112, 284, 598, 664, 697, 1126, 1330
Hpy188III TCNNGA 7 cut(s) 239, 398, 425, 641, 847, 1237, 1481
Hpy8I GTNNAC 2 cut(s) 48, 544
HpyAV CCTTC 6 cut(s) 52, 603, 944, 1049, 1387, 1538
HpyCH4III ACNGT 5 cut(s) 187, 443, 809, 853, 1163
HpyCH4IV ACGT 2 cut(s) 1246, 1293
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 656, 771, 1483
HpyF3I CTNAG 2 cut(s) 661, 1329
HpySE526I ACGT 2 cut(s) 1246, 1293
HspAI GCGC 2 cut(s) 137, 166
Kpn2I TCCGGA 1 cut(s) 640
Ksp22I TGATCA 2 cut(s) 427, 559
KspAI GTTAAC 1 cut(s) 48
Kzo9I GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1432, 1534
LmnI GCTCC 1 cut(s) 902
Lsp1109I GCAGC 2 cut(s) 562, 739
LweI GCATC 2 cut(s) 1369, 1486
MaeI CTAG 3 cut(s) 1073, 1481, 1512
MaeII ACGT 2 cut(s) 1246, 1293
MaeIII GTNAC 1 cut(s) 453
MalI GATC 8 cut(s) 114, 423, 429, 561, 1071, 1128, 1434, 1536
MboI GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1432, 1534
MboII GAAGA 5 cut(s) 260, 317, 1367, 1388, 1579
MfeI CAATTG 1 cut(s) 1274
MflI RGATCY 3 cut(s) 112, 1126, 1432
MhlI GDGCHC 1 cut(s) 546
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 1 cut(s) 1172
Mph1103I ATGCAT 3 cut(s) 77, 839, 1479
MroI TCCGGA 1 cut(s) 640
MseI TTAA 2 cut(s) 47, 1173
MslI CAYNNNNRTG 4 cut(s) 80, 185, 570, 1472
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 5 cut(s) 362, 641, 647, 884, 900
MspR9I CCNGG 3 cut(s) 257, 363, 884
MunI CAATTG 1 cut(s) 1274
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 4 cut(s) 581, 656, 771, 1483
NciI CCSGG 2 cut(s) 363, 884
NcoI CCATGG 2 cut(s) 906, 1095
NdeII GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1432, 1534
NlaIV GGNNCC 9 cut(s) 69, 98, 317, 645, 827, 904, 932, 1101, 1289
NsbI TGCGCA 1 cut(s) 167
NsiI ATGCAT 3 cut(s) 77, 839, 1479
NspI RCATGY 1 cut(s) 1529
OliI CACNNNNGTG 1 cut(s) 185
PagI TCATGA 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 1234
PciI ACATGT 1 cut(s) 1525
PfeI GAWTC 6 cut(s) 102, 599, 712, 734, 1018, 1385
PflMI CCANNNNNTGG 2 cut(s) 93, 822
PkrI GCNGC 3 cut(s) 175, 577, 729
PleI GAGTC 1 cut(s) 659
PpsI GAGTC 1 cut(s) 659
Ppu21I YACGTR 1 cut(s) 1247
PpuMI RGGWCCY 1 cut(s) 931
PscI ACATGT 1 cut(s) 1525
PsiI TTATAA 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 931
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 9 cut(s) 69, 98, 317, 645, 827, 904, 932, 1101, 1289
PspPI GGNCC 2 cut(s) 316, 931
PspPPI RGGWCCY 1 cut(s) 931
PsuI RGATCY 3 cut(s) 112, 1126, 1432
RsaI GTAC 2 cut(s) 434, 1260
RsaNI GTAC 2 cut(s) 433, 1259
RseI CAYNNNNRTG 4 cut(s) 80, 185, 570, 1472
SaqAI TTAA 2 cut(s) 47, 1173
SatI GCNGC 3 cut(s) 174, 576, 728
Sau3AI GATC 8 cut(s) 112, 421, 427, 559, 1069, 1126, 1432, 1534
Sau96I GGNCC 2 cut(s) 316, 931
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 3 cut(s) 257, 363, 884
SduI GDGCHC 1 cut(s) 546
SfaNI GCATC 2 cut(s) 1369, 1486
SfcI CTRYAG 1 cut(s) 613
SinI GGWCC 2 cut(s) 316, 931
SmiMI CAYNNNNRTG 4 cut(s) 80, 185, 570, 1472
SmlI CTYRAG 2 cut(s) 845, 1487
SmoI CTYRAG 2 cut(s) 845, 1487
SsiI CCGC 7 cut(s) 171, 174, 268, 417, 793, 864, 890
SspI AATATT 1 cut(s) 1062
SspMI CTAG 3 cut(s) 1073, 1481, 1512
StyD4I CCNGG 3 cut(s) 255, 361, 882
StyI CCWWGG 3 cut(s) 579, 906, 1095
TaaI ACNGT 5 cut(s) 187, 443, 809, 853, 1163
TaiI ACGT 2 cut(s) 1249, 1296
TaqI TCGA 1 cut(s) 715
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 6 cut(s) 102, 599, 712, 734, 1018, 1385
Tru1I TTAA 2 cut(s) 47, 1173
Tru9I TTAA 2 cut(s) 47, 1173
TscAI CASTG 3 cut(s) 751, 1395, 1465
TseI GCWGC 2 cut(s) 575, 727
TspDTI ATGAA 8 cut(s) 227, 642, 962, 1017, 1202, 1293, 1439, 1536
TspRI CASTG 3 cut(s) 751, 1395, 1465
Van91I CCANNNNNTGG 2 cut(s) 93, 822
VneI GTGCAC 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 316, 931
XapI RAATTY 4 cut(s) 7, 19, 128, 1190
XbaI TCTAGA 1 cut(s) 1480
XceI RCATGY 1 cut(s) 1529
XcmI CCANNNNNNNNNTGG 2 cut(s) 184, 1465
XspI CTAG 3 cut(s) 1073, 1481, 1512
Zsp2I ATGCAT 3 cut(s) 77, 839, 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.