Rh2DG161000

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000947
Physical Location & Seq
Forward (+)
13702869 .. 13709690
6822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG161000.1

Sequence Viewer

Length: 1218 bp
ATGGAGAAAGACTTGGTGACATGCATGAAGGTGTCGAAGTCATGGCACCGAATATCATCTGATGTTTGGGCGCGGAGATTTTGGGCGCAATCGGCTCCTTTGGGTCTATACTTCCGCACCATCCAGACTCTGTTGAGTCCTGACATGCGTAGCCCCACGGACAAGGAGCCATCTTATTTAAAGATATTTGTAATATTCTACGTGTGCAACCCCATCACCACACAACAAGTCCCCGTACCTAAAGCCTCGGTCCACCAAAACCATGAGCATTTTTGTGCTGCCTTGGCTTTTGACCCTTCGGAATCTCCTCATCACTATAGAGTTGTGAGGATTGATTACTCTGAATCCGTTTCATCAAACTCAAACTCAGCCCTGCTTGATATCTTTTGGTCTGAATACGGACAGTGGGTTAGACATAGGTTGCAGCTTGACCCAGATTTTACTGAGGGGTTTGAGAAGGTGAAGTTGTGCAGGCAATTTGTTTACATGCGGGGCAAGTTATACAGCATAGCCATGTCATGGAAGCTTTTAGGCATTGACCTCAAAACTATTGAAGCTTCGACCCTTGAACTTCCTCTTCATGATGCCGATAAGACCGGAGCAATGGGATGTCTCGGTGTGTCGATGGACCTTCTATGCTATATAAAGTGTATGCATGATACTTCTGATGATCTGCACGATCTGGTAGTTTGGTACTACGATGATCGTTGTGAATCTGGTGAATGGATTCTCAGATATAGTGTTTCTTGCTGCTTGTTGGGATGTGCAATTAAGTATCAAGGGTTTAAGTATGATGATACCGTGGAGCCTTATGCTATAAGTTCAAGTTCTGATGTCTTGTTCTTTGGCACTTCCAATTTGATTTGTTGCTACCATCTCAAAAGCGAAAAGATCAAATTCGTGTGTCATGCAGATTGCTGTACAATAGATACACCCGGTAATTTCTGGATATTGCGTGCTTTCTTCGTACCTTTCTTCCAGTTCCGAAAGAGAAATAGCGGCTCTACTGTCTTACCAATGGTTTCAGATGACATAGAGATTTTGACTGCGGAAACAGTATGCGAAGAAATGAAAGATGATGAATATGTTGAAGTGATAAACGTTAATTTATGTGATCGTCATGAAGAGCCATTGCCGCCGTTGATTTATCCTAGTGAAATTACTGAAATGCATGTGGAGTTTAGTCATCCGGAGACTTCAAGTTCCGATGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

405

Amino Acids

46.68

Weight (kDa)

5.11

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_AT5G49610-like PF23635 68 - 303 2.3e-07 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 45
AccB7I CCANNNNNTGG 1 cut(s) 519
AccII CGCG 1 cut(s) 73
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 6 cut(s) 73, 115, 490, 999, 1049, 1136
AclI AACGTT 1 cut(s) 1101
AcsI RAATTY 1 cut(s) 896
AfaI GTAC 4 cut(s) 237, 695, 922, 969
AfiI CCNNNNNNNGG 1 cut(s) 519
AflIII ACRYGT 1 cut(s) 201
AgsI TTSAA 5 cut(s) 554, 569, 825, 1091, 1200
AluBI AGCT 3 cut(s) 427, 526, 557
AluI AGCT 3 cut(s) 427, 526, 557
Alw26I GTCTC 2 cut(s) 617, 1187
AlwNI CAGNNNCTG 1 cut(s) 130
Aor13HI TCCGGA 1 cut(s) 1189
ApeKI GCWGC 3 cut(s) 278, 424, 750
ApoI RAATTY 1 cut(s) 896
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 2 cut(s) 73, 88
AspS9I GGNCC 2 cut(s) 250, 628
AsuC2I CCSGG 1 cut(s) 936
AsuHPI GGTGA 4 cut(s) 28, 208, 472, 731
AvaII GGWCC 2 cut(s) 250, 628
BanI GGYRCC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 265, 436, 737
BccI CCATC 5 cut(s) 128, 178, 221, 619, 882
BceAI ACGGC 1 cut(s) 1123
BcnI CCSGG 1 cut(s) 936
BcoDI GTCTC 2 cut(s) 617, 1187
BfaI CTAG 1 cut(s) 1152
BfmI CTRYAG 1 cut(s) 316
BisI GCNGC 5 cut(s) 279, 425, 751, 1000, 1136
BlsI GCNGC 5 cut(s) 280, 426, 752, 1001, 1137
Bme1390I CCNGG 1 cut(s) 936
Bme18I GGWCC 2 cut(s) 250, 628
BmgT120I GGNCC 2 cut(s) 250, 628
BmiI GGNNCC 4 cut(s) 47, 96, 168, 807
BmrFI CCNGG 1 cut(s) 936
BmsI GCATC 1 cut(s) 574
BpuMI CCSGG 1 cut(s) 936
BsaAI YACGTR 1 cut(s) 202
BsaJI CCNNGG 4 cut(s) 156, 246, 282, 801
BsaWI WCCGGW 2 cut(s) 596, 1189
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 1 cut(s) 979
Bse3DI GCAATG 2 cut(s) 609, 1130
BseAI TCCGGA 1 cut(s) 1189
BseDI CCNNGG 4 cut(s) 156, 246, 282, 801
BseGI GGATG 4 cut(s) 120, 614, 767, 1186
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMI GCAATG 2 cut(s) 609, 1130
BseMII CTCAG 3 cut(s) 381, 435, 745
BseNI ACTGG 1 cut(s) 979
BseRI GAGGAG 1 cut(s) 297
BseXI GCAGC 3 cut(s) 265, 436, 737
BsgI GTGCAG 2 cut(s) 490, 659
Bsh1236I CGCG 1 cut(s) 73
BshNI GGYRCC 1 cut(s) 45
BsiSI CCGG 3 cut(s) 597, 936, 1190
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 2 cut(s) 617, 1187
BsmFI GGGAC 1 cut(s) 215
Bsp13I TCCGGA 1 cut(s) 1189
Bsp1407I TGTACA 1 cut(s) 920
Bsp143I GATC 5 cut(s) 670, 679, 703, 891, 1114
BspACI CCGC 6 cut(s) 73, 115, 490, 999, 1049, 1136
BspCNI CTCAG 3 cut(s) 380, 436, 744
BspEI TCCGGA 1 cut(s) 1189
BspFNI CGCG 1 cut(s) 73
BspHI TCATGA 2 cut(s) 580, 1120
BspLI GGNNCC 4 cut(s) 47, 96, 168, 807
BspQI GCTCTTC 1 cut(s) 1119
BspT107I GGYRCC 1 cut(s) 45
BsrDI GCAATG 2 cut(s) 609, 1130
BsrGI TGTACA 1 cut(s) 920
BsrI ACTGG 1 cut(s) 979
BssECI CCNNGG 4 cut(s) 156, 246, 282, 801
BssMI GATC 5 cut(s) 670, 679, 703, 891, 1114
BssT1I CCWWGG 1 cut(s) 282
Bst4CI ACNGT 4 cut(s) 405, 802, 1009, 1057
Bst6I CTCTTC 2 cut(s) 582, 1119
BstAUI TGTACA 1 cut(s) 920
BstBAI YACGTR 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 473, 957
BstDEI CTNAG 3 cut(s) 367, 444, 731
BstDSI CCRYGG 2 cut(s) 156, 801
BstF5I GGATG 4 cut(s) 120, 614, 767, 1186
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 2 cut(s) 73, 88
BstKTI GATC 5 cut(s) 673, 682, 706, 894, 1117
BstMAI GTCTC 2 cut(s) 617, 1187
BstMBI GATC 5 cut(s) 670, 679, 703, 891, 1114
BstMWI GCNNNNNNNGC 3 cut(s) 92, 284, 1135
BstNSI RCATGY 4 cut(s) 24, 148, 490, 1175
BstSCI CCNGG 1 cut(s) 934
BstSFI CTRYAG 1 cut(s) 316
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 3 cut(s) 265, 436, 737
BtgI CCRYGG 2 cut(s) 156, 801
BtsCI GGATG 4 cut(s) 120, 614, 767, 1186
BtsIMutI CAGTG 1 cut(s) 410
Cac8I GCNNGC 2 cut(s) 473, 957
CaiI CAGNNNCTG 1 cut(s) 130
CciI TCATGA 2 cut(s) 580, 1120
CfoI GCGC 2 cut(s) 73, 88
Cfr13I GGNCC 2 cut(s) 250, 628
Csp6I GTAC 4 cut(s) 236, 694, 921, 968
CviQI GTAC 4 cut(s) 236, 694, 921, 968
DdeI CTNAG 3 cut(s) 367, 444, 731
DpnI GATC 5 cut(s) 672, 681, 705, 893, 1116
DpnII GATC 5 cut(s) 670, 679, 703, 891, 1114
DraI TTTAAA 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 582, 1119
EarI CTCTTC 2 cut(s) 582, 1119
Eco130I CCWWGG 1 cut(s) 282
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 2 cut(s) 250, 628
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 1 cut(s) 282
EcoT22I ATGCAT 3 cut(s) 26, 657, 1173
ErhI CCWWGG 1 cut(s) 282
FaqI GGGAC 1 cut(s) 215
FauI CCCGC 1 cut(s) 483
Fnu4HI GCNGC 5 cut(s) 279, 425, 751, 1000, 1136
FokI GGATG 4 cut(s) 107, 621, 774, 1173
Fsp4HI GCNGC 5 cut(s) 279, 425, 751, 1000, 1136
FspBI CTAG 1 cut(s) 1152
GlaI GCGC 2 cut(s) 72, 87
GluI GCNGC 5 cut(s) 279, 425, 751, 1000, 1136
HapII CCGG 3 cut(s) 597, 936, 1190
HhaI GCGC 2 cut(s) 73, 88
Hin6I GCGC 2 cut(s) 71, 86
HinP1I GCGC 2 cut(s) 71, 86
HindIII AAGCTT 2 cut(s) 524, 555
HinfI GANTC 6 cut(s) 127, 136, 302, 344, 713, 727
HpaII CCGG 3 cut(s) 597, 936, 1190
HphI GGTGA 4 cut(s) 28, 208, 472, 731
Hpy166II GTNNAC 2 cut(s) 253, 484
Hpy188III TCNNGA 6 cut(s) 124, 140, 581, 946, 1121, 1190
Hpy8I GTNNAC 2 cut(s) 253, 484
HpyAV CCTTC 4 cut(s) 22, 306, 451, 641
HpyCH4III ACNGT 4 cut(s) 405, 802, 1009, 1057
HpyCH4IV ACGT 2 cut(s) 201, 1101
HpyCH4V TGCA 9 cut(s) 24, 207, 424, 471, 655, 676, 767, 911, 1171
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 284, 1135
HpyF3I CTNAG 3 cut(s) 367, 444, 731
HpySE526I ACGT 2 cut(s) 201, 1101
HspAI GCGC 2 cut(s) 71, 86
Kpn2I TCCGGA 1 cut(s) 1189
Kzo9I GATC 5 cut(s) 670, 679, 703, 891, 1114
LguI GCTCTTC 1 cut(s) 1119
LmnI GCTCC 4 cut(s) 100, 166, 599, 805
Lsp1109I GCAGC 3 cut(s) 265, 436, 737
LweI GCATC 1 cut(s) 574
MaeI CTAG 1 cut(s) 1152
MaeII ACGT 2 cut(s) 201, 1101
MaeIII GTNAC 1 cut(s) 16
MalI GATC 5 cut(s) 672, 681, 705, 893, 1116
MboI GATC 5 cut(s) 670, 679, 703, 891, 1114
MboII GAAGA 5 cut(s) 569, 955, 967, 1076, 1136
MluCI AATT 7 cut(s) 476, 768, 856, 896, 940, 1105, 1158
MlyI GAGTC 2 cut(s) 121, 145
MnlI CCTC 6 cut(s) 256, 318, 321, 439, 551, 585
Mph1103I ATGCAT 3 cut(s) 26, 657, 1173
MroI TCCGGA 1 cut(s) 1189
MroXI GAANNNNTTC 1 cut(s) 726
MseI TTAA 4 cut(s) 179, 771, 786, 1104
MslI CAYNNNNRTG 3 cut(s) 29, 273, 512
MspI CCGG 3 cut(s) 597, 936, 1190
MspR9I CCNGG 1 cut(s) 936
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 3 cut(s) 92, 284, 1135
NciI CCSGG 1 cut(s) 936
NdeII GATC 5 cut(s) 670, 679, 703, 891, 1114
NlaIV GGNNCC 4 cut(s) 47, 96, 168, 807
NmuCI GTSAC 1 cut(s) 16
NsiI ATGCAT 3 cut(s) 26, 657, 1173
NspI RCATGY 4 cut(s) 24, 148, 490, 1175
PagI TCATGA 2 cut(s) 580, 1120
PciSI GCTCTTC 1 cut(s) 1119
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 4 cut(s) 302, 344, 713, 727
PflMI CCANNNNNTGG 1 cut(s) 519
PkrI GCNGC 5 cut(s) 280, 426, 752, 1001, 1137
PleI GAGTC 2 cut(s) 121, 144
PpsI GAGTC 2 cut(s) 121, 144
Ppu21I YACGTR 1 cut(s) 202
Psp1406I AACGTT 1 cut(s) 1101
PspN4I GGNNCC 4 cut(s) 47, 96, 168, 807
PspPI GGNCC 2 cut(s) 250, 628
PstNI CAGNNNCTG 1 cut(s) 130
RsaI GTAC 4 cut(s) 237, 695, 922, 969
RsaNI GTAC 4 cut(s) 236, 694, 921, 968
RseI CAYNNNNRTG 3 cut(s) 29, 273, 512
SapI GCTCTTC 1 cut(s) 1119
SaqAI TTAA 4 cut(s) 179, 771, 786, 1104
SatI GCNGC 5 cut(s) 279, 425, 751, 1000, 1136
Sau3AI GATC 5 cut(s) 670, 679, 703, 891, 1114
Sau96I GGNCC 2 cut(s) 250, 628
SchI GAGTC 2 cut(s) 121, 145
ScrFI CCNGG 1 cut(s) 936
SfaNI GCATC 1 cut(s) 574
SfcI CTRYAG 1 cut(s) 316
SinI GGWCC 2 cut(s) 250, 628
SmiMI CAYNNNNRTG 3 cut(s) 29, 273, 512
Sse9I AATT 7 cut(s) 476, 768, 856, 896, 940, 1105, 1158
SsiI CCGC 6 cut(s) 73, 115, 490, 999, 1049, 1136
SspI AATATT 1 cut(s) 195
SspMI CTAG 1 cut(s) 1152
StyD4I CCNGG 1 cut(s) 934
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 4 cut(s) 405, 802, 1009, 1057
TaiI ACGT 2 cut(s) 204, 1104
TaqI TCGA 3 cut(s) 35, 560, 623
TaqII GACCGA 1 cut(s) 238
TasI AATT 7 cut(s) 476, 768, 856, 896, 940, 1105, 1158
TatI WGTACW 1 cut(s) 920
TauI GCSGC 2 cut(s) 1002, 1138
TfiI GAWTC 4 cut(s) 302, 344, 713, 727
Tru1I TTAA 4 cut(s) 179, 771, 786, 1104
Tru9I TTAA 4 cut(s) 179, 771, 786, 1104
TscAI CASTG 1 cut(s) 410
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 3 cut(s) 278, 424, 750
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 6 cut(s) 41, 342, 569, 1085, 1095, 1137
TspGWI ACGGA 3 cut(s) 173, 337, 414
TspRI CASTG 1 cut(s) 410
Van91I CCANNNNNTGG 1 cut(s) 519
VpaK11BI GGWCC 2 cut(s) 250, 628
XapI RAATTY 1 cut(s) 896
XceI RCATGY 4 cut(s) 24, 148, 490, 1175
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 1 cut(s) 1152
Zsp2I ATGCAT 3 cut(s) 26, 657, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.