RLG00000017152

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000947
Physical Location & Seq
Forward (+)
13417621 .. 13419051
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017152

Sequence Viewer

Length: 1431 bp
ATGGGGAATTTATGCGAAGATTTGTGGGTTGAGATCTTGGGTAGAATGTCATCTGAGAAAGACTTGGTGCCATGCATGAGTGTGTCCAAGTTATGGCACAGAATAGCATCAGATCTTTGGGTGAGGAGATTTTGGGCGCAGTCGCCTCCTTTGGGTGTTTACTTTCGCACCATCCAGAATCCTTTGAGTCCTTTCATGCTCACTATGGTGGACAAAGTGCCGTCTTATTTGCCTAGCATTAATTATATCTCTCTATATAATTATCATAACATGGAAGATAACCTCGAAAGCACTAGCGGTTATTATCTCTGTACGTTATTTAATGGCGCAAATCACTTGTTTAAGCGTGTTTGGCAATATTATCGACATTATAAACACGATCACGATGATCATGATGAATATCTACACGGCTGCAATGGATTACTTCTGTTATTGAAATCGGGAACTAATCAGTTTTATGTTTGCAATCCCATTACCACACAACAAGTCCCCGTGCCTAAAGCTTGTGTCCACCAAAACCATGAGCATTTTTGTGCTGCCTTGGCTTTTGACCCTTCTGAATCTCCTCAGCACTACAGAGTTGTGAGGATTGATTACTCTGAATCCGTTTCATCTACCTCAAACTCTGTCGTGTTTGACATATTTTCATCTAAATACGGACAGTGGGTTCGACATAGATTGCAACTTGACCCAGGTTTTACTAAGGGGTTCGAAGAGGTAAAGCTGTGCAGGCAATTTTTTTACTTGCGGGGCAAGTTATATAGCTTAGCCATGTCATGGAAGATTTTATGCATTGACCTCAAAACTGTTGAAGCTTCAGCCCTTGAACTTCCTGTCCCGGAGGCCAATAAGAACGGAGCGATGGGATGTCTTGGGGTGTCGATGGACCTTCTCTGCTATATAAAGCGTATGCCTTCCAGTAGGTTTCACGATCTGGTAGTTTGGTACTATGATGATCATTGTGAATCTAGTGAATGGACTCGCAGATACAATGTTAGTTGCGGCTTGTTGGGATATGGAATAAAGTATCAAGGGTATGATTATGATGATACTGTGGAGCCCTATGCAATAAGTCCTGGTTCTGATATCTTGTTCTTTGGCACTCCCAACGTGATTAGTTGCTACCACCTCAAAAGCAAAAAGTACAAACCCGTGTGGCAACTTCCTTCCTCTAAAATTGTACCTGATTACTTCTGGGTATTGCGTGCTTGCTTCATACCTTTCTACCAGTTGGGAAACAAAGCAGTGGTTCCTGAGGGGAAGGGCATAGAAATCTTGACTGCAGAAGCACAATGTGAGGAAGAGAAAGATGTTGACAAAGTTGAAGTGATAAACGTCAAATTATTTGATCGCCATAAAAAGCCGTTGCCACCGTTGATTTATCCTAGCGAAATTACTGAAATGTATTTGGAATTTGAGTTTCCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

477

Amino Acids

55.26

Weight (kDa)

6.19

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 113 - 336 7.3e-13 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 125 - 292 3.9e-09 F-box associated beta propeller domain
FBA_3 PF08268 126 - 212 2.8e-06 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 133 - 287 3.2e-07 KIB1-4 beta-propeller
Beta-prop_AT5G49610-like PF23635 138 - 279 3.7e-06 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 67
AccB7I CCANNNNNTGG 2 cut(s) 93, 777
AciI CCGC 3 cut(s) 297, 748, 1002
AcsI RAATTY 2 cut(s) 7, 1412
AcuI CTGAAG 1 cut(s) 801
AdeI CACNNNGTG 1 cut(s) 1295
AfaI GTAC 4 cut(s) 313, 947, 1145, 1182
AfiI CCNNNNNNNGG 3 cut(s) 93, 152, 777
AgsI TTSAA 4 cut(s) 436, 812, 827, 1325
AjnI CCWGG 2 cut(s) 691, 1075
AleI CACNNNNGTG 1 cut(s) 206
AloI GAACNNNNNNTCC 4 cut(s) 651, 683, 819, 851
AluBI AGCT 4 cut(s) 503, 724, 765, 815
AluI AGCT 4 cut(s) 503, 724, 765, 815
AoxI GGCC 1 cut(s) 843
ApeKI GCWGC 2 cut(s) 411, 536
ApoI RAATTY 2 cut(s) 7, 1412
ArsI GACNNNNNNTTYG 2 cut(s) 651, 683
AseI ATTAAT 1 cut(s) 240
AspLEI GCGC 2 cut(s) 139, 329
AspS9I GGNCC 1 cut(s) 886
AsuC2I CCSGG 1 cut(s) 839
AsuHPI GGTGA 1 cut(s) 133
AsuII TTCGAA 1 cut(s) 711
AvaII GGWCC 1 cut(s) 886
AxyI CCTNAGG 1 cut(s) 1254
BanI GGYRCC 1 cut(s) 67
BanII GRGCYC 1 cut(s) 1062
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 2 cut(s) 398, 523
BccI CCATC 3 cut(s) 179, 856, 877
BceAI ACGGC 3 cut(s) 205, 424, 1348
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 2 cut(s) 693, 1077
BclI TGATCA 2 cut(s) 388, 955
BcnI CCSGG 1 cut(s) 839
BfaI CTAG 4 cut(s) 234, 294, 969, 1386
BfmI CTRYAG 2 cut(s) 574, 1281
BglII AGATCT 2 cut(s) 33, 112
BisI GCNGC 3 cut(s) 412, 537, 1003
BlpI GCTNAGC 1 cut(s) 766
BlsI GCNGC 3 cut(s) 413, 538, 1004
Bme1390I CCNGG 3 cut(s) 693, 839, 1077
Bme18I GGWCC 1 cut(s) 886
BmgT120I GGNCC 1 cut(s) 886
BmiI GGNNCC 3 cut(s) 69, 1059, 1251
BmrFI CCNGG 3 cut(s) 693, 839, 1077
BmsI GCATC 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 766
Bpu14I TTCGAA 1 cut(s) 711
BpuMI CCSGG 1 cut(s) 839
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 540, 691
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 152, 777
Bse1I ACTGG 2 cut(s) 918, 1228
Bse21I CCTNAGG 1 cut(s) 1254
Bse3DI GCAATG 1 cut(s) 421
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 2 cut(s) 693, 1077
BseDI CCNNGG 2 cut(s) 540, 691
BseGI GGATG 2 cut(s) 171, 872
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 3 cut(s) 93, 152, 777
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 3 cut(s) 45, 581, 1245
BseNI ACTGG 2 cut(s) 918, 1228
BseRI GAGGAG 2 cut(s) 139, 555
BseXI GCAGC 2 cut(s) 398, 523
BsgI GTGCAG 1 cut(s) 748
BshFI GGCC 1 cut(s) 845
BshNI GGYRCC 1 cut(s) 67
BsiSI CCGG 1 cut(s) 839
BslFI GGGAC 2 cut(s) 473, 821
BslI CCNNNNNNNGG 3 cut(s) 93, 152, 777
BsmFI GGGAC 2 cut(s) 473, 821
BsnI GGCC 1 cut(s) 845
Bsp119I TTCGAA 1 cut(s) 711
Bsp1286I GDGCHC 1 cut(s) 1062
Bsp143I GATC 7 cut(s) 33, 112, 379, 388, 931, 955, 1348
Bsp1720I GCTNAGC 1 cut(s) 766
BspACI CCGC 3 cut(s) 297, 748, 1002
BspANI GGCC 1 cut(s) 845
BspCNI CTCAG 3 cut(s) 46, 580, 1246
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 3 cut(s) 69, 1059, 1251
BspMAI CTGCAG 1 cut(s) 1285
BspT104I TTCGAA 1 cut(s) 711
BspT107I GGYRCC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 421
BsrI ACTGG 2 cut(s) 918, 1228
BssECI CCNNGG 2 cut(s) 540, 691
BssMI GATC 7 cut(s) 33, 112, 379, 388, 931, 955, 1348
BssT1I CCWWGG 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 693, 1077
Bst4CI ACNGT 4 cut(s) 663, 808, 1054, 1374
Bst6I CTCTTC 2 cut(s) 708, 1296
BstBI TTCGAA 1 cut(s) 711
BstC8I GCNNGC 3 cut(s) 731, 1206, 1210
BstDEI CTNAG 5 cut(s) 54, 567, 702, 766, 1254
BstF5I GGATG 2 cut(s) 171, 872
BstHHI GCGC 2 cut(s) 139, 329
BstKTI GATC 7 cut(s) 36, 115, 382, 391, 934, 958, 1351
BstMBI GATC 7 cut(s) 33, 112, 379, 388, 931, 955, 1348
BstMWI GCNNNNNNNGC 3 cut(s) 352, 542, 730
BstNI CCWGG 2 cut(s) 693, 1077
BstSCI CCNGG 3 cut(s) 691, 837, 1075
BstSFI CTRYAG 2 cut(s) 574, 1281
BstV1I GCAGC 2 cut(s) 398, 523
BstX2I RGATCY 2 cut(s) 33, 112
BstYI RGATCY 2 cut(s) 33, 112
Bsu36I CCTNAGG 1 cut(s) 1254
BsuRI GGCC 1 cut(s) 845
BtgZI GCGATG 1 cut(s) 875
BtsCI GGATG 2 cut(s) 171, 872
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 2 cut(s) 668, 1251
Cac8I GCNNGC 3 cut(s) 731, 1206, 1210
CciI TCATGA 1 cut(s) 391
CfoI GCGC 2 cut(s) 139, 329
Cfr13I GGNCC 1 cut(s) 886
Csp6I GTAC 4 cut(s) 312, 946, 1144, 1181
CviAII CATG 8 cut(s) 72, 76, 196, 271, 392, 521, 772, 777
CviQI GTAC 4 cut(s) 312, 946, 1144, 1181
DdeI CTNAG 5 cut(s) 54, 567, 702, 766, 1254
DpnI GATC 7 cut(s) 35, 114, 381, 390, 933, 957, 1350
DpnII GATC 7 cut(s) 33, 112, 379, 388, 931, 955, 1348
DraIII CACNNNGTG 1 cut(s) 1295
Eam1104I CTCTTC 2 cut(s) 708, 1296
EarI CTCTTC 2 cut(s) 708, 1296
Eco130I CCWWGG 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 1062
Eco32I GATATC 1 cut(s) 1087
Eco47I GGWCC 1 cut(s) 886
Eco57I CTGAAG 1 cut(s) 801
Eco81I CCTNAGG 1 cut(s) 1254
EcoRII CCWGG 2 cut(s) 691, 1075
EcoRV GATATC 1 cut(s) 1087
EcoT14I CCWWGG 1 cut(s) 540
EcoT22I ATGCAT 2 cut(s) 77, 794
EcoT38I GRGCYC 1 cut(s) 1062
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 8 cut(s) 75, 79, 199, 274, 395, 524, 775, 780
FaqI GGGAC 2 cut(s) 473, 821
FatI CATG 8 cut(s) 71, 75, 195, 270, 391, 520, 771, 776
FauI CCCGC 1 cut(s) 741
FbaI TGATCA 2 cut(s) 388, 955
Fnu4HI GCNGC 3 cut(s) 412, 537, 1003
FokI GGATG 2 cut(s) 158, 879
FriOI GRGCYC 1 cut(s) 1062
Fsp4HI GCNGC 3 cut(s) 412, 537, 1003
FspBI CTAG 4 cut(s) 234, 294, 969, 1386
GlaI GCGC 2 cut(s) 138, 328
GluI GCNGC 3 cut(s) 412, 537, 1003
HaeIII GGCC 1 cut(s) 845
HapII CCGG 1 cut(s) 839
HhaI GCGC 2 cut(s) 139, 329
Hin1II CATG 8 cut(s) 75, 79, 199, 274, 395, 524, 775, 780
Hin6I GCGC 2 cut(s) 137, 327
HinP1I GCGC 2 cut(s) 137, 327
HincII GTYRAC 1 cut(s) 1315
HindII GTYRAC 1 cut(s) 1315
HindIII AAGCTT 2 cut(s) 501, 813
HinfI GANTC 6 cut(s) 178, 187, 560, 602, 965, 979
HpaII CCGG 1 cut(s) 839
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 4 cut(s) 160, 211, 511, 1315
Hpy188I TCNGA 5 cut(s) 55, 112, 559, 601, 1084
Hpy188III TCNNGA 7 cut(s) 175, 383, 392, 441, 929, 1253, 1276
Hpy8I GTNNAC 4 cut(s) 160, 211, 511, 1315
HpyAV CCTTC 5 cut(s) 564, 899, 924, 1176, 1255
HpyCH4III ACNGT 4 cut(s) 663, 808, 1054, 1374
HpyCH4IV ACGT 3 cut(s) 314, 1110, 1335
HpyCH4V TGCA 8 cut(s) 75, 414, 465, 682, 729, 792, 1067, 1283
HpyF10VI GCNNNNNNNGC 3 cut(s) 352, 542, 730
HpyF3I CTNAG 5 cut(s) 54, 567, 702, 766, 1254
HpySE526I ACGT 3 cut(s) 314, 1110, 1335
Hsp92II CATG 8 cut(s) 75, 79, 199, 274, 395, 524, 775, 780
HspAI GCGC 2 cut(s) 137, 327
Ksp22I TGATCA 2 cut(s) 388, 955
Kzo9I GATC 7 cut(s) 33, 112, 379, 388, 931, 955, 1348
LmnI GCTCC 2 cut(s) 857, 1057
Lsp1109I GCAGC 2 cut(s) 398, 523
LweI GCATC 1 cut(s) 116
MaeI CTAG 4 cut(s) 234, 294, 969, 1386
MaeII ACGT 3 cut(s) 314, 1110, 1335
MalI GATC 7 cut(s) 35, 114, 381, 390, 933, 957, 1350
MboI GATC 7 cut(s) 33, 112, 379, 388, 931, 955, 1348
MboII GAAGA 5 cut(s) 29, 287, 725, 793, 1313
MflI RGATCY 2 cut(s) 33, 112
MhlI GDGCHC 1 cut(s) 1062
MluCI AATT 9 cut(s) 7, 241, 259, 734, 1176, 1340, 1392, 1412, 1426
MlyI GAGTC 2 cut(s) 196, 973
Mph1103I ATGCAT 2 cut(s) 77, 794
MseI TTAA 4 cut(s) 240, 321, 342, 1429
MslI CAYNNNNRTG 3 cut(s) 80, 206, 531
MspI CCGG 1 cut(s) 839
MspR9I CCNGG 3 cut(s) 693, 839, 1077
MvaI CCWGG 2 cut(s) 693, 1077
MwoI GCNNNNNNNGC 3 cut(s) 352, 542, 730
NciI CCSGG 1 cut(s) 839
NdeII GATC 7 cut(s) 33, 112, 379, 388, 931, 955, 1348
NlaIII CATG 8 cut(s) 75, 79, 199, 274, 395, 524, 775, 780
NlaIV GGNNCC 3 cut(s) 69, 1059, 1251
NsiI ATGCAT 2 cut(s) 77, 794
NspV TTCGAA 1 cut(s) 711
OliI CACNNNNGTG 1 cut(s) 206
PagI TCATGA 1 cut(s) 391
PfeI GAWTC 4 cut(s) 178, 560, 602, 965
PflMI CCANNNNNTGG 2 cut(s) 93, 777
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 3 cut(s) 413, 538, 1004
PleI GAGTC 2 cut(s) 195, 973
PpsI GAGTC 2 cut(s) 195, 973
PshBI ATTAAT 1 cut(s) 240
PsiI TTATAA 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 691, 1075
PspGI CCWGG 2 cut(s) 691, 1075
PspN4I GGNNCC 3 cut(s) 69, 1059, 1251
PspPI GGNCC 1 cut(s) 886
PstI CTGCAG 1 cut(s) 1285
PsuI RGATCY 2 cut(s) 33, 112
RsaI GTAC 4 cut(s) 313, 947, 1145, 1182
RsaNI GTAC 4 cut(s) 312, 946, 1144, 1181
RseI CAYNNNNRTG 3 cut(s) 80, 206, 531
SaqAI TTAA 4 cut(s) 240, 321, 342, 1429
SatI GCNGC 3 cut(s) 412, 537, 1003
Sau3AI GATC 7 cut(s) 33, 112, 379, 388, 931, 955, 1348
Sau96I GGNCC 1 cut(s) 886
SchI GAGTC 2 cut(s) 196, 973
ScrFI CCNGG 3 cut(s) 693, 839, 1077
SduI GDGCHC 1 cut(s) 1062
SfaNI GCATC 1 cut(s) 116
SfcI CTRYAG 2 cut(s) 574, 1281
SfuI TTCGAA 1 cut(s) 711
SinI GGWCC 1 cut(s) 886
SmiMI CAYNNNNRTG 3 cut(s) 80, 206, 531
Sse9I AATT 9 cut(s) 7, 241, 259, 734, 1176, 1340, 1392, 1412, 1426
SsiI CCGC 3 cut(s) 297, 748, 1002
SspI AATATT 1 cut(s) 359
SspMI CTAG 4 cut(s) 234, 294, 969, 1386
StyD4I CCNGG 3 cut(s) 691, 837, 1075
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 4 cut(s) 663, 808, 1054, 1374
TaiI ACGT 3 cut(s) 317, 1113, 1338
TaqI TCGA 5 cut(s) 285, 364, 670, 711, 881
TasI AATT 9 cut(s) 7, 241, 259, 734, 1176, 1340, 1392, 1412, 1426
TatI WGTACW 1 cut(s) 1143
TauI GCSGC 1 cut(s) 1005
TfiI GAWTC 4 cut(s) 178, 560, 602, 965
Tru1I TTAA 4 cut(s) 240, 321, 342, 1429
Tru9I TTAA 4 cut(s) 240, 321, 342, 1429
TscAI CASTG 2 cut(s) 668, 1251
TseI GCWGC 2 cut(s) 411, 536
TspDTI ATGAA 5 cut(s) 184, 411, 600, 636, 1204
TspGWI ACGGA 3 cut(s) 595, 672, 870
TspRI CASTG 2 cut(s) 668, 1251
Van91I CCANNNNNTGG 2 cut(s) 93, 777
VpaK11BI GGWCC 1 cut(s) 886
VspI ATTAAT 1 cut(s) 240
XapI RAATTY 2 cut(s) 7, 1412
XspI CTAG 4 cut(s) 234, 294, 969, 1386
Zsp2I ATGCAT 2 cut(s) 77, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.