pycom02g12120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000947
Physical Location & Seq
Forward (+)
8691353 .. 8691751
399 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12120.1

Sequence Viewer

Length: 399 bp
ATGGACAGAGTCTCGGTGCTGAAGAGGTCCACGATAAGCACTGTAGGACGAAGAGAAGACTCCATTCCAAGTATGGCTGACCGAAACGCGGGTCGTACTTCACGCATCATGACTGCAAGGACTGTAGCGACAGTAGCGTCGGGGCCAACACAGCCGGAAATGTCGGGCGGCGGGAGTTCAATAATGTCAAAAAATTTGGGGGTGGCGGCTGCCTTGAGGAGCTCAGACTCAGTCTTGGAAGCAGGAGATGGGATGGCGAAGATCGTCACGGTGAAATTCCGGTGGGTGACAAGTCGTTTTGCAAGTTCGAGGACGGGGATGAGGTGGCCGAGGCCTGGGCTGCAAAGTATGGCAGCATGTGGCTGTGAGCTCATGGTAGACGGAGAGCAAAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

14.05

Weight (kDa)

11.01

Isoelectric Point (pI)

64.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 378
AccII CGCG 1 cut(s) 89
AciI CCGC 4 cut(s) 89, 168, 171, 206
AcoI YGGCCR 1 cut(s) 326
AcsI RAATTY 2 cut(s) 193, 275
AcuI CTGAAG 1 cut(s) 41
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 2 cut(s) 88, 335
AgsI TTSAA 1 cut(s) 180
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 2 cut(s) 222, 370
AluI AGCT 2 cut(s) 222, 370
Alw21I GWGCWC 2 cut(s) 224, 372
Alw26I GTCTC 1 cut(s) 16
AoxI GGCC 3 cut(s) 143, 326, 332
ApeKI GCWGC 3 cut(s) 209, 340, 353
ApoI RAATTY 2 cut(s) 193, 275
ArsI GACNNNNNNTTYG 2 cut(s) 76, 108
AspS9I GGNCC 2 cut(s) 27, 143
AsuHPI GGTGA 2 cut(s) 283, 298
AvaII GGWCC 1 cut(s) 27
BanII GRGCYC 2 cut(s) 224, 372
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 224, 372
BbvI GCAGC 3 cut(s) 196, 327, 365
BccI CCATC 2 cut(s) 242, 247
BciT130I CCWGG 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 16
BfmI CTRYAG 2 cut(s) 42, 123
BisI GCNGC 5 cut(s) 169, 207, 210, 341, 354
BlsI GCNGC 5 cut(s) 170, 208, 211, 342, 355
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 27, 143
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 329, 335
BsaWI WCCGGW 1 cut(s) 279
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 335
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 329, 335
BseGI GGATG 2 cut(s) 258, 324
BseLI CCNNNNNNNGG 2 cut(s) 88, 335
BseMII CTCAG 2 cut(s) 237, 243
BseRI GAGGAG 1 cut(s) 232
BseXI GCAGC 3 cut(s) 196, 327, 365
Bsh1236I CGCG 1 cut(s) 89
BshFI GGCC 3 cut(s) 145, 328, 334
BsiHKAI GWGCWC 2 cut(s) 224, 372
BsiSI CCGG 2 cut(s) 155, 280
BslI CCNNNNNNNGG 2 cut(s) 88, 335
BsmAI GTCTC 1 cut(s) 16
BsnI GGCC 3 cut(s) 145, 328, 334
Bsp1286I GDGCHC 2 cut(s) 224, 372
Bsp143I GATC 1 cut(s) 261
BspACI CCGC 4 cut(s) 89, 168, 171, 206
BspANI GGCC 3 cut(s) 145, 328, 334
BspCNI CTCAG 2 cut(s) 236, 242
BspFNI CGCG 1 cut(s) 89
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 144
BssECI CCNNGG 2 cut(s) 329, 335
BssMI GATC 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 4 cut(s) 43, 124, 133, 271
Bst6I CTCTTC 2 cut(s) 17, 46
BstDEI CTNAG 2 cut(s) 223, 229
BstF5I GGATG 2 cut(s) 258, 324
BstFNI CGCG 1 cut(s) 89
BstKTI GATC 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 3 cut(s) 134, 151, 340
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 2 cut(s) 42, 123
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 3 cut(s) 196, 327, 365
BstV2I GAAGAC 1 cut(s) 63
BsuRI GGCC 3 cut(s) 145, 328, 334
BtsCI GGATG 2 cut(s) 258, 324
BtsIMutI CAGTG 1 cut(s) 39
CciI TCATGA 1 cut(s) 108
Cfr13I GGNCC 2 cut(s) 27, 143
CseI GACGC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 96
CviAII CATG 3 cut(s) 109, 357, 373
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 2 cut(s) 223, 229
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
DrdI GACNNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 326
Eam1104I CTCTTC 2 cut(s) 17, 46
EarI CTCTTC 2 cut(s) 17, 46
Ecl136II GAGCTC 2 cut(s) 222, 370
Eco147I AGGCCT 1 cut(s) 334
Eco24I GRGCYC 2 cut(s) 224, 372
Eco47I GGWCC 1 cut(s) 27
Eco53kI GAGCTC 2 cut(s) 222, 370
Eco57I CTGAAG 1 cut(s) 41
EcoICRI GAGCTC 2 cut(s) 222, 370
EcoRII CCWGG 1 cut(s) 334
EcoT38I GRGCYC 2 cut(s) 224, 372
FaeI CATG 3 cut(s) 112, 360, 376
FaiI YATR 5 cut(s) 74, 110, 350, 358, 374
FatI CATG 3 cut(s) 108, 356, 372
FauI CCCGC 2 cut(s) 82, 164
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 5 cut(s) 169, 207, 210, 341, 354
FokI GGATG 2 cut(s) 265, 331
FriOI GRGCYC 2 cut(s) 224, 372
Fsp4HI GCNGC 5 cut(s) 169, 207, 210, 341, 354
GluI GCNGC 5 cut(s) 169, 207, 210, 341, 354
HaeIII GGCC 3 cut(s) 145, 328, 334
HapII CCGG 2 cut(s) 155, 280
HgaI GACGC 1 cut(s) 126
Hin1II CATG 3 cut(s) 112, 360, 376
HinfI GANTC 3 cut(s) 9, 59, 227
HpaII CCGG 2 cut(s) 155, 280
HphI GGTGA 2 cut(s) 283, 298
Hpy166II GTNNAC 2 cut(s) 30, 379
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 2 cut(s) 30, 379
Hpy99I CGWCG 1 cut(s) 142
HpyCH4III ACNGT 4 cut(s) 43, 124, 133, 271
HpyCH4V TGCA 3 cut(s) 116, 302, 343
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 151, 340
HpyF3I CTNAG 2 cut(s) 223, 229
Hsp92II CATG 3 cut(s) 112, 360, 376
Kzo9I GATC 1 cut(s) 261
LmnI GCTCC 1 cut(s) 219
LpnPI CCDG 5 cut(s) 168, 228, 293, 321, 348
Lsp1109I GCAGC 3 cut(s) 196, 327, 365
LweI GCATC 1 cut(s) 114
MaeIII GTNAC 2 cut(s) 265, 286
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 4 cut(s) 34, 63, 68, 271
MhlI GDGCHC 2 cut(s) 224, 372
MluCI AATT 2 cut(s) 193, 275
MlyI GAGTC 3 cut(s) 18, 53, 221
MnlI CCTC 5 cut(s) 18, 210, 303, 315, 324
MspI CCGG 2 cut(s) 155, 280
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 3 cut(s) 134, 151, 340
NdeII GATC 1 cut(s) 261
NlaIII CATG 3 cut(s) 112, 360, 376
NlaIV GGNNCC 1 cut(s) 144
NmeAIII GCCGAG 1 cut(s) 354
NmuCI GTSAC 2 cut(s) 265, 286
NspI RCATGY 1 cut(s) 360
PagI TCATGA 1 cut(s) 108
PceI AGGCCT 1 cut(s) 334
PflFI GACNNNGTC 2 cut(s) 8, 230
PkrI GCNGC 5 cut(s) 170, 208, 211, 342, 355
PleI GAGTC 3 cut(s) 17, 53, 221
PpsI GAGTC 3 cut(s) 17, 53, 221
Psp124BI GAGCTC 2 cut(s) 224, 372
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 2 cut(s) 27, 143
PsyI GACNNNGTC 2 cut(s) 8, 230
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SacI GAGCTC 2 cut(s) 224, 372
SatI GCNGC 5 cut(s) 169, 207, 210, 341, 354
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 2 cut(s) 27, 143
SchI GAGTC 3 cut(s) 18, 53, 221
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 2 cut(s) 224, 372
SetI ASST 4 cut(s) 29, 224, 326, 372
SfaNI GCATC 1 cut(s) 114
SfcI CTRYAG 2 cut(s) 42, 123
SinI GGWCC 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 2 cut(s) 193, 275
SseBI AGGCCT 1 cut(s) 334
SsiI CCGC 4 cut(s) 89, 168, 171, 206
SstI GAGCTC 2 cut(s) 224, 372
StuI AGGCCT 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 334
TaaI ACNGT 4 cut(s) 43, 124, 133, 271
TaqI TCGA 1 cut(s) 308
TaqII GACCGA 1 cut(s) 96
TasI AATT 2 cut(s) 193, 275
TauI GCSGC 2 cut(s) 171, 209
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 2 cut(s) 265, 286
TseI GCWGC 3 cut(s) 209, 340, 353
Tsp45I GTSAC 2 cut(s) 265, 286
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 1 cut(s) 46
Tth111I GACNNNGTC 2 cut(s) 8, 230
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 2 cut(s) 193, 275
XceI RCATGY 1 cut(s) 360
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.