Rroxscaffold_2G00140470

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000947
Physical Location & Seq
Reverse (-)
78695589 .. 78697031
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140470.1

Sequence Viewer

Length: 1443 bp
ATGGGGAATTTATGCGAGGATTTGTGGGTTGAGATCTTGGGTAGACTGTCATCTGAGAAAGACTTGGTGCCATGCATGAGTGTGTCCAAGTTATGGCACAGAATAGCATCAGATCTTTGGGTGAGGAGATTTTGGGCGCAGTCGCCTCCTTTGGGTGTTTACTTTCGCACCATTCAGAATCCTTCGAGTCCTTCCATGCTCACCACGGTGGACAAGGTGCTGTCTTATTTGCCTAGCATTAATTATATCTCTCTATATAATTATCATCACATGGATGATAATCTCGAAAGCACGAGCGGTTATCATCTCTGTACGTTATTTAATGGCTTAAGCTACCTGTTTGAGTGCTATAAGCGAATTTGGCAATTTTATCGACATTATAAACACGATCACGATGATCATGATGATCAGTATCTACACGGCTGCAATGGATTACTTCTGTTATTGAAATCGGGAACTTATCAGTTCTATGTGTGCAATCCCATTACCACACAACAACTCCCCGTACCTAAAGCTTGTGTCCACCAAAACCAGGAGCATTTTTGTGCCGCCTTGGCTTTTGACCCTTCTGAATCTCCTCATCACTACAGAGTTGTGAGGATCGATTTCTCTGGATCCGTTTCATCTACCTCAAACTCTGTCATGTTTGACATATTTTCATCTAAATACGGACAGTGGGTTCGACATAGGTTGCAACTTGACCCAGGTTTTACTGAGGGGTTTAAAAAGGTAAAGCTGTGCAGGCAATTTTTTTATTTGCGGGGCAAGTTATATAGCTTAGCCATGTCATGGAAGATTTTATGCATTGACCTCAAAACTGTTGAAGCTTCAGCCCTTGAACTTCCTGTCCCGGAGGCCAATAAGACCGGAGCGTTGGGATGTCTTGGGGTGTCGATGGACCTTCTCTGCTATATAAAGCGTATGCCTTCCGGTAAGTTTCACCATCTGGTAGTTTGGTACTATGATGATCGTTGTGAATCTAGTGAATGGACTCGCAGATATAATGTTAGTTGCGGCTTGTTGGGATATGGAATAAAGTATCAAGGGTATGATTATGATGATACTGTGGAACCCTATGCAATAAGTCCTAGTTCTGATGTCTTGTTCTTTGGCACTCCCAACCTGATTAGTTGCTACCACCTCAAAAGCGAAAAGTACAAGCCCGTGTGTCACCTATCTCCCTCTAAAATTGTACCTGATTACTTCTGGGTATTGCGTGCTTGCTTCGTACCTTTCTACCGGTTGGGAAACAAAGCAGTGGTTCCTGAGGAGAAGGGCATAGAAATCTTGACTGCAGAAGCACAATGTGAAGAAGAGAAAGATGTTGACAAAGTTGAAGTGATAAACGTCAAATTATGTGATCGTCATAAAAAGCCGTTGCCACCCTTGATTTATCCTAGCGAAATTACTGAAATGTATTTGGAATTTGAGTGTCCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

480

Amino Acids

55.59

Weight (kDa)

6.44

Isoelectric Point (pI)

55.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 6 - 44 1.5e-06 F-box-like
b-prop_At3g26010-like PF24750 128 - 340 4.7e-11 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 134 - 303 8.8e-09 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 135 - 291 4.6e-06 KIB1-4 beta-propeller
Beta-prop_AT5G49610-like PF23635 142 - 283 5.6e-06 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 381
AccB1I GGYRCC 1 cut(s) 67
AccB7I CCANNNNNTGG 2 cut(s) 93, 789
AccBSI CCGCTC 1 cut(s) 297
AccI GTMKAC 1 cut(s) 43
AciI CCGC 4 cut(s) 297, 549, 760, 1014
AclWI GGATC 3 cut(s) 608, 609, 622
AcsI RAATTY 3 cut(s) 7, 357, 1424
AcuI CTGAAG 1 cut(s) 813
AdeI CACNNNGTG 1 cut(s) 1307
AfaI GTAC 6 cut(s) 313, 507, 959, 1157, 1194, 1230
AfiI CCNNNNNNNGG 4 cut(s) 93, 152, 532, 789
AflII CTTAAG 1 cut(s) 328
AgeI ACCGGT 1 cut(s) 1239
AgsI TTSAA 4 cut(s) 448, 824, 839, 1337
AjnI CCWGG 2 cut(s) 531, 703
AleI CACNNNNGTG 1 cut(s) 206
AloI GAACNNNNNNTCC 4 cut(s) 663, 695, 831, 863
AluBI AGCT 5 cut(s) 333, 515, 736, 777, 827
AluI AGCT 5 cut(s) 333, 515, 736, 777, 827
AlwI GGATC 3 cut(s) 608, 609, 622
AoxI GGCC 1 cut(s) 855
ApeKI GCWGC 1 cut(s) 423
ApoI RAATTY 3 cut(s) 7, 357, 1424
ArsI GACNNNNNNTTYG 2 cut(s) 663, 695
AseI ATTAAT 1 cut(s) 240
AsiGI ACCGGT 1 cut(s) 1239
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 898
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 4 cut(s) 133, 193, 932, 1163
AvaII GGWCC 1 cut(s) 898
AxyI CCTNAGG 1 cut(s) 1266
BamHI GGATCC 1 cut(s) 614
BanI GGYRCC 1 cut(s) 67
BauI CACGAG 1 cut(s) 292
BbvI GCAGC 1 cut(s) 410
BccI CCATC 2 cut(s) 889, 951
BceAI ACGGC 2 cut(s) 436, 1360
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BciT130I CCWGG 2 cut(s) 533, 705
BclI TGATCA 2 cut(s) 397, 406
BcnI CCSGG 1 cut(s) 851
BfaI CTAG 4 cut(s) 234, 981, 1089, 1398
BfmI CTRYAG 2 cut(s) 586, 1293
BfrI CTTAAG 1 cut(s) 328
BglI GCCNNNNNGGC 1 cut(s) 554
BglII AGATCT 2 cut(s) 33, 112
BisI GCNGC 3 cut(s) 424, 549, 1015
BlpI GCTNAGC 1 cut(s) 778
BlsI GCNGC 3 cut(s) 425, 550, 1016
Bme1390I CCNGG 3 cut(s) 533, 705, 851
Bme18I GGWCC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 898
BmiI GGNNCC 4 cut(s) 69, 616, 1071, 1263
BmrFI CCNGG 3 cut(s) 533, 705, 851
BmsI GCATC 1 cut(s) 116
Bpu1102I GCTNAGC 1 cut(s) 778
BpuMI CCSGG 1 cut(s) 851
Bsa29I ATCGAT 1 cut(s) 603
BsaBI GATNNNNATC 2 cut(s) 279, 411
BsaJI CCNNGG 3 cut(s) 204, 552, 703
BsaWI WCCGGW 3 cut(s) 866, 929, 1239
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 152, 532, 789
Bse118I RCCGGY 1 cut(s) 1239
Bse21I CCTNAGG 1 cut(s) 1266
Bse3DI GCAATG 1 cut(s) 433
Bse8I GATNNNNATC 2 cut(s) 279, 411
BseBI CCWGG 2 cut(s) 533, 705
BseCI ATCGAT 1 cut(s) 603
BseDI CCNNGG 3 cut(s) 204, 552, 703
BseGI GGATG 2 cut(s) 280, 884
BseJI GATNNNNATC 2 cut(s) 279, 411
BseLI CCNNNNNNNGG 4 cut(s) 93, 152, 532, 789
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 3 cut(s) 45, 705, 1257
BseRI GAGGAG 3 cut(s) 139, 567, 1283
BseXI GCAGC 1 cut(s) 410
BsgI GTGCAG 1 cut(s) 760
BshFI GGCC 1 cut(s) 857
BshNI GGYRCC 1 cut(s) 67
BshTI ACCGGT 1 cut(s) 1239
BshVI ATCGAT 1 cut(s) 603
BsiSI CCGG 4 cut(s) 851, 867, 930, 1240
BslFI GGGAC 2 cut(s) 833, 1419
BslI CCNNNNNNNGG 4 cut(s) 93, 152, 532, 789
BsmFI GGGAC 2 cut(s) 833, 1419
BsnI GGCC 1 cut(s) 857
Bsp143I GATC 9 cut(s) 33, 112, 388, 397, 406, 600, 614, 967, 1360
Bsp1720I GCTNAGC 1 cut(s) 778
BspACI CCGC 4 cut(s) 297, 549, 760, 1014
BspANI GGCC 1 cut(s) 857
BspCNI CTCAG 3 cut(s) 46, 706, 1258
BspDI ATCGAT 1 cut(s) 603
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 4 cut(s) 69, 616, 1071, 1263
BspMAI CTGCAG 1 cut(s) 1297
BspPI GGATC 3 cut(s) 608, 609, 622
BspT107I GGYRCC 1 cut(s) 67
BspTI CTTAAG 1 cut(s) 328
BsrBI CCGCTC 1 cut(s) 297
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 1239
BssAI RCCGGY 1 cut(s) 1239
BssECI CCNNGG 3 cut(s) 204, 552, 703
BssMI GATC 9 cut(s) 33, 112, 388, 397, 406, 600, 614, 967, 1360
BssSI CACGAG 1 cut(s) 292
BssT1I CCWWGG 1 cut(s) 552
Bst2BI CACGAG 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 533, 705
Bst4CI ACNGT 5 cut(s) 48, 208, 675, 820, 1066
Bst6I CTCTTC 1 cut(s) 1308
BstAFI CTTAAG 1 cut(s) 328
BstC8I GCNNGC 3 cut(s) 743, 1218, 1222
BstDEI CTNAG 4 cut(s) 54, 714, 778, 1266
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 280, 884
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 9 cut(s) 36, 115, 391, 400, 409, 603, 617, 970, 1363
BstMBI GATC 9 cut(s) 33, 112, 388, 397, 406, 600, 614, 967, 1360
BstMWI GCNNNNNNNGC 3 cut(s) 361, 554, 742
BstNI CCWGG 2 cut(s) 533, 705
BstSCI CCNGG 3 cut(s) 531, 703, 849
BstSFI CTRYAG 2 cut(s) 586, 1293
BstV1I GCAGC 1 cut(s) 410
BstX2I RGATCY 3 cut(s) 33, 112, 614
BstYI RGATCY 3 cut(s) 33, 112, 614
Bsu15I ATCGAT 1 cut(s) 603
Bsu36I CCTNAGG 1 cut(s) 1266
BsuRI GGCC 1 cut(s) 857
BsuTUI ATCGAT 1 cut(s) 603
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 2 cut(s) 280, 884
BtsI GCAGTG 1 cut(s) 1263
BtsIMutI CAGTG 2 cut(s) 680, 1263
Cac8I GCNNGC 3 cut(s) 743, 1218, 1222
CciI TCATGA 1 cut(s) 400
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 1239
Cfr13I GGNCC 1 cut(s) 898
ClaI ATCGAT 1 cut(s) 603
Csp6I GTAC 6 cut(s) 312, 506, 958, 1156, 1193, 1229
CspAI ACCGGT 1 cut(s) 1239
CviAII CATG 8 cut(s) 72, 76, 196, 271, 401, 643, 784, 789
CviQI GTAC 6 cut(s) 312, 506, 958, 1156, 1193, 1229
DdeI CTNAG 4 cut(s) 54, 714, 778, 1266
DpnI GATC 9 cut(s) 35, 114, 390, 399, 408, 602, 616, 969, 1362
DpnII GATC 9 cut(s) 33, 112, 388, 397, 406, 600, 614, 967, 1360
DraI TTTAAA 1 cut(s) 724
DraIII CACNNNGTG 1 cut(s) 1307
Eam1104I CTCTTC 1 cut(s) 1308
EarI CTCTTC 1 cut(s) 1308
Eco130I CCWWGG 1 cut(s) 552
Eco47I GGWCC 1 cut(s) 898
Eco57I CTGAAG 1 cut(s) 813
Eco81I CCTNAGG 1 cut(s) 1266
EcoRII CCWGG 2 cut(s) 531, 703
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 2 cut(s) 77, 806
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 8 cut(s) 75, 79, 199, 274, 404, 646, 787, 792
FaqI GGGAC 2 cut(s) 833, 1419
FatI CATG 8 cut(s) 71, 75, 195, 270, 400, 642, 783, 788
FauI CCCGC 1 cut(s) 753
FbaI TGATCA 2 cut(s) 397, 406
FblI GTMKAC 1 cut(s) 43
Fnu4HI GCNGC 3 cut(s) 424, 549, 1015
FokI GGATG 2 cut(s) 287, 891
Fsp4HI GCNGC 3 cut(s) 424, 549, 1015
FspBI CTAG 4 cut(s) 234, 981, 1089, 1398
GlaI GCGC 1 cut(s) 138
GluI GCNGC 3 cut(s) 424, 549, 1015
HaeIII GGCC 1 cut(s) 857
HapII CCGG 4 cut(s) 851, 867, 930, 1240
HhaI GCGC 1 cut(s) 139
Hin1II CATG 8 cut(s) 75, 79, 199, 274, 404, 646, 787, 792
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 1327
HindII GTYRAC 1 cut(s) 1327
HindIII AAGCTT 2 cut(s) 513, 825
HinfI GANTC 5 cut(s) 178, 187, 572, 977, 991
HpaII CCGG 4 cut(s) 851, 867, 930, 1240
HphI GGTGA 4 cut(s) 133, 193, 932, 1163
Hpy166II GTNNAC 5 cut(s) 44, 160, 211, 523, 1327
Hpy188I TCNGA 5 cut(s) 55, 112, 177, 571, 1096
Hpy188III TCNNGA 7 cut(s) 284, 392, 401, 453, 612, 1265, 1288
Hpy8I GTNNAC 5 cut(s) 44, 160, 211, 523, 1327
HpyAV CCTTC 6 cut(s) 192, 201, 576, 911, 936, 1267
HpyCH4III ACNGT 5 cut(s) 48, 208, 675, 820, 1066
HpyCH4IV ACGT 2 cut(s) 314, 1347
HpyCH4V TGCA 8 cut(s) 75, 426, 477, 694, 741, 804, 1079, 1295
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 554, 742
HpyF3I CTNAG 4 cut(s) 54, 714, 778, 1266
HpySE526I ACGT 2 cut(s) 314, 1347
Hsp92II CATG 8 cut(s) 75, 79, 199, 274, 404, 646, 787, 792
HspAI GCGC 1 cut(s) 137
Ksp22I TGATCA 2 cut(s) 397, 406
Kzo9I GATC 9 cut(s) 33, 112, 388, 397, 406, 600, 614, 967, 1360
LmnI GCTCC 2 cut(s) 535, 869
Lsp1109I GCAGC 1 cut(s) 410
LweI GCATC 1 cut(s) 116
MaeI CTAG 4 cut(s) 234, 981, 1089, 1398
MaeII ACGT 2 cut(s) 314, 1347
MaeIII GTNAC 1 cut(s) 1169
MalI GATC 9 cut(s) 35, 114, 390, 399, 408, 602, 616, 969, 1362
MbiI CCGCTC 1 cut(s) 297
MboI GATC 9 cut(s) 33, 112, 388, 397, 406, 600, 614, 967, 1360
MboII GAAGA 3 cut(s) 805, 1322, 1325
MflI RGATCY 3 cut(s) 33, 112, 614
MlyI GAGTC 2 cut(s) 196, 985
Mph1103I ATGCAT 2 cut(s) 77, 806
MseI TTAA 5 cut(s) 240, 321, 329, 723, 1441
MslI CAYNNNNRTG 4 cut(s) 80, 206, 273, 543
MspCI CTTAAG 1 cut(s) 328
MspI CCGG 4 cut(s) 851, 867, 930, 1240
MspR9I CCNGG 3 cut(s) 533, 705, 851
MvaI CCWGG 2 cut(s) 533, 705
MwoI GCNNNNNNNGC 3 cut(s) 361, 554, 742
NciI CCSGG 1 cut(s) 851
NdeII GATC 9 cut(s) 33, 112, 388, 397, 406, 600, 614, 967, 1360
NlaIII CATG 8 cut(s) 75, 79, 199, 274, 404, 646, 787, 792
NlaIV GGNNCC 4 cut(s) 69, 616, 1071, 1263
NmuCI GTSAC 1 cut(s) 1169
NsiI ATGCAT 2 cut(s) 77, 806
OliI CACNNNNGTG 1 cut(s) 206
PagI TCATGA 1 cut(s) 400
PfeI GAWTC 3 cut(s) 178, 572, 977
PflMI CCANNNNNTGG 2 cut(s) 93, 789
PfoI TCCNGGA 1 cut(s) 849
PinAI ACCGGT 1 cut(s) 1239
PkrI GCNGC 3 cut(s) 425, 550, 1016
PleI GAGTC 2 cut(s) 195, 985
PpsI GAGTC 2 cut(s) 195, 985
PshBI ATTAAT 1 cut(s) 240
PsiI TTATAA 1 cut(s) 381
Psp6I CCWGG 2 cut(s) 531, 703
PspGI CCWGG 2 cut(s) 531, 703
PspN4I GGNNCC 4 cut(s) 69, 616, 1071, 1263
PspPI GGNCC 1 cut(s) 898
PstI CTGCAG 1 cut(s) 1297
PsuI RGATCY 3 cut(s) 33, 112, 614
RsaI GTAC 6 cut(s) 313, 507, 959, 1157, 1194, 1230
RsaNI GTAC 6 cut(s) 312, 506, 958, 1156, 1193, 1229
RseI CAYNNNNRTG 4 cut(s) 80, 206, 273, 543
SaqAI TTAA 5 cut(s) 240, 321, 329, 723, 1441
SatI GCNGC 3 cut(s) 424, 549, 1015
Sau3AI GATC 9 cut(s) 33, 112, 388, 397, 406, 600, 614, 967, 1360
Sau96I GGNCC 1 cut(s) 898
SchI GAGTC 2 cut(s) 196, 985
ScrFI CCNGG 3 cut(s) 533, 705, 851
SfaNI GCATC 1 cut(s) 116
SfcI CTRYAG 2 cut(s) 586, 1293
SinI GGWCC 1 cut(s) 898
SmiMI CAYNNNNRTG 4 cut(s) 80, 206, 273, 543
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
SsiI CCGC 4 cut(s) 297, 549, 760, 1014
SspMI CTAG 4 cut(s) 234, 981, 1089, 1398
StyD4I CCNGG 3 cut(s) 531, 703, 849
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 5 cut(s) 48, 208, 675, 820, 1066
TaiI ACGT 2 cut(s) 317, 1350
TaqI TCGA 6 cut(s) 185, 285, 373, 603, 682, 893
TatI WGTACW 1 cut(s) 1155
TauI GCSGC 2 cut(s) 551, 1017
TfiI GAWTC 3 cut(s) 178, 572, 977
Tru1I TTAA 5 cut(s) 240, 321, 329, 723, 1441
Tru9I TTAA 5 cut(s) 240, 321, 329, 723, 1441
TscAI CASTG 2 cut(s) 680, 1263
TseFI GTSAC 1 cut(s) 1169
TseI GCWGC 1 cut(s) 423
Tsp45I GTSAC 1 cut(s) 1169
TspDTI ATGAA 2 cut(s) 612, 648
TspGWI ACGGA 2 cut(s) 607, 684
TspRI CASTG 2 cut(s) 680, 1263
Van91I CCANNNNNTGG 2 cut(s) 93, 789
Vha464I CTTAAG 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 898
VspI ATTAAT 1 cut(s) 240
XapI RAATTY 3 cut(s) 7, 357, 1424
XmiI GTMKAC 1 cut(s) 43
XspI CTAG 4 cut(s) 234, 981, 1089, 1398
Zsp2I ATGCAT 2 cut(s) 77, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.