Rmu_sc0000474.1_g000001

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000474.1
OG0000947
Physical Location & Seq
Reverse (-)
14239 .. 15462
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000474.1_g000001.1.cds

Sequence Viewer

Length: 1224 bp
atgcacttgggtagactatcatcggagaaggacttggtgccatgcatgagcgtgtccaggttatggcaccgattaacatctggtctttgggtgcagagattttgggcgcagtcccctcctttgggtcttttcttccgcaccgccaccaccagcaccaccaccgtagacaaggtattgcatcgtatgaattatatctctttatataattatcatgccatggaagataacctgcaaagcactcgttgttgcggaggtcaattttatgacaaagaatcgtataggtccaaaaagtcccattggcagtattttgatcgacaatggtttaagccgggtgccactgctgaatccactgatcgtgatgagtacctaaatggttgcaatggtttagttttgttaatgaaatcagcaacttatcagttttatgtttgcaatcccatcacaagacaacaagttgccatacctaaagcctgtgtccaccaaaaccgtgatgatcagcatttttgtgctgctttggcttttgacccttccgaatctgctcatcacttcagagttgttagaattgattattctgcaccctactcagctgtgctggatatattctcgtctgaatatggactgtgggttcgacattggctgcaaattgacccaggttttactgggggattcaaaagctccaagttgtgcaggcattttgtttacttgcgaggtaggctatacagcatagccctatcctggaaccttttatgcattgacctcaatactgttgcagcttctgcccttgaacttcctcttctagggaatgaaaagaccggagccatgggatgtcttggggtgtcgatgaatagtctctgctatataaagcgaatgctcgataattcgaaggactttcacaatctggcagtttggtataatgatgatgaatctggggaatggattctcagatatagtgtttcttgtcgcatattgggatacggattcgagtctttaggttatgactacgatgattccatggaaccctgtgcaataagtccaacttcagatctcttgttctttggcaatctcaacttgatttgttgtatccaccttaaaagcgaaaagatgaaattcgtttgcgaaacaatctctaaaatagatccacctgcttctttcttgacattacgtgcatgcttcgtacctttctacactcagttgaacaagaggaatgacgtctccactccattgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

407

Amino Acids

46.67

Weight (kDa)

7.82

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1147
AatII GACGTC 1 cut(s) 1209
Acc36I ACCTGC 2 cut(s) 237, 1147
AccB1I GGYRCC 3 cut(s) 37, 66, 332
AccB7I CCANNNNNTGG 1 cut(s) 63
AccI GTMKAC 2 cut(s) 13, 165
AciI CCGC 3 cut(s) 136, 141, 249
AclWI GGATC 1 cut(s) 1127
AcsI RAATTY 1 cut(s) 1103
AcuI CTGAAG 2 cut(s) 529, 1020
AcyI GRCGYC 1 cut(s) 1206
AfaI GTAC 2 cut(s) 365, 1173
AfiI CCNNNNNNNGG 5 cut(s) 63, 121, 122, 732, 794
AgsI TTSAA 3 cut(s) 667, 782, 1192
AjnI CCWGG 3 cut(s) 56, 646, 731
AloI GAACNNNNNNTCC 2 cut(s) 606, 638
AluBI AGCT 3 cut(s) 584, 672, 770
AluI AGCT 3 cut(s) 584, 672, 770
Alw26I GTCTC 2 cut(s) 851, 1213
AlwI GGATC 1 cut(s) 1127
AlwNI CAGNNNCTG 1 cut(s) 773
ApeKI GCWGC 3 cut(s) 506, 634, 767
ApoI RAATTY 1 cut(s) 1103
ArsI GACNNNNNNTTYG 2 cut(s) 606, 638
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 282
AsuC2I CCSGG 1 cut(s) 330
AsuII TTCGAA 1 cut(s) 878
AvaII GGWCC 1 cut(s) 282
BanI GGYRCC 3 cut(s) 37, 66, 332
BbvI GCAGC 3 cut(s) 493, 621, 779
BccI CCATC 1 cut(s) 443
BcgI CGANNNNNNTGC 2 cut(s) 221, 255
BciT130I CCWGG 3 cut(s) 58, 648, 733
BciVI GTATCC 2 cut(s) 962, 1088
BclI TGATCA 1 cut(s) 490
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 2 cut(s) 851, 1213
BfaI CTAG 1 cut(s) 794
BfuAI ACCTGC 2 cut(s) 237, 1147
BfuI GTATCC 2 cut(s) 962, 1088
BglII AGATCT 1 cut(s) 1039
BisI GCNGC 3 cut(s) 507, 635, 768
BlsI GCNGC 3 cut(s) 508, 636, 769
Bme1390I CCNGG 4 cut(s) 58, 330, 648, 733
Bme18I GGWCC 1 cut(s) 282
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 6 cut(s) 39, 68, 334, 737, 814, 1014
BmrFI CCNGG 4 cut(s) 58, 330, 648, 733
BmrI ACTGGG 1 cut(s) 666
BmsI GCATC 1 cut(s) 187
BmuI ACTGGG 1 cut(s) 666
Bpu14I TTCGAA 1 cut(s) 878
BpuMI CCSGG 1 cut(s) 330
BsaAI YACGTR 1 cut(s) 1160
BsaBI GATNNNNATC 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 1206
BsaJI CCNNGG 4 cut(s) 216, 646, 816, 1008
BsaWI WCCGGW 1 cut(s) 809
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 121, 122, 732, 794
Bse1I ACTGG 1 cut(s) 661
Bse3DI GCAATG 1 cut(s) 385
Bse8I GATNNNNATC 1 cut(s) 76
BseBI CCWGG 3 cut(s) 58, 648, 733
BseDI CCNNGG 4 cut(s) 216, 646, 816, 1008
BseGI GGATG 1 cut(s) 827
BseJI GATNNNNATC 1 cut(s) 76
BseLI CCNNNNNNNGG 5 cut(s) 63, 121, 122, 732, 794
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 3 cut(s) 594, 952, 1199
BseNI ACTGG 1 cut(s) 661
BseXI GCAGC 3 cut(s) 493, 621, 779
BsgI GTGCAG 3 cut(s) 113, 555, 703
BshNI GGYRCC 3 cut(s) 37, 66, 332
BsiSI CCGG 2 cut(s) 329, 810
BslFI GGGAC 2 cut(s) 97, 277
BslI CCNNNNNNNGG 5 cut(s) 63, 121, 122, 732, 794
BsmAI GTCTC 2 cut(s) 851, 1213
BsmBI CGTCTC 1 cut(s) 1213
BsmFI GGGAC 2 cut(s) 97, 277
BsmI GAATGC 1 cut(s) 870
Bsp119I TTCGAA 1 cut(s) 878
Bsp143I GATC 5 cut(s) 310, 352, 490, 1039, 1132
Bsp19I CCATGG 3 cut(s) 216, 816, 1008
BspACI CCGC 3 cut(s) 136, 141, 249
BspCNI CTCAG 3 cut(s) 593, 951, 1198
BspLI GGNNCC 6 cut(s) 39, 68, 334, 737, 814, 1014
BspMI ACCTGC 2 cut(s) 237, 1147
BspPI GGATC 1 cut(s) 1127
BspT104I TTCGAA 1 cut(s) 878
BspT107I GGYRCC 3 cut(s) 37, 66, 332
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 1 cut(s) 661
BssECI CCNNGG 4 cut(s) 216, 646, 816, 1008
BssMI GATC 5 cut(s) 310, 352, 490, 1039, 1132
BssNI GRCGYC 1 cut(s) 1206
BssT1I CCWWGG 3 cut(s) 216, 816, 1008
Bst2UI CCWGG 3 cut(s) 58, 648, 733
Bst4CI ACNGT 4 cut(s) 163, 485, 618, 763
Bst6I CTCTTC 1 cut(s) 795
BstACI GRCGYC 1 cut(s) 1206
BstAPI GCANNNNNTGC 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 1160
BstBI TTCGAA 1 cut(s) 878
BstC8I GCNNGC 2 cut(s) 686, 1165
BstDEI CTNAG 3 cut(s) 580, 938, 1185
BstDSI CCRYGG 3 cut(s) 216, 816, 1008
BstENI CCTNNNNNAGG 1 cut(s) 792
BstF5I GGATG 1 cut(s) 827
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 5 cut(s) 313, 355, 493, 1042, 1135
BstMAI GTCTC 2 cut(s) 851, 1213
BstMBI GATC 5 cut(s) 310, 352, 490, 1039, 1132
BstMWI GCNNNNNNNGC 3 cut(s) 512, 709, 773
BstNI CCWGG 3 cut(s) 58, 648, 733
BstNSI RCATGY 1 cut(s) 1167
BstSCI CCNGG 4 cut(s) 56, 328, 646, 731
BstV1I GCAGC 3 cut(s) 493, 621, 779
BstX2I RGATCY 2 cut(s) 1039, 1132
BstYI RGATCY 2 cut(s) 1039, 1132
BsuI GTATCC 2 cut(s) 962, 1088
BtgI CCRYGG 3 cut(s) 216, 816, 1008
BtsCI GGATG 1 cut(s) 827
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 336, 348
BveI ACCTGC 2 cut(s) 237, 1147
Cac8I GCNNGC 2 cut(s) 686, 1165
CaiI CAGNNNCTG 1 cut(s) 773
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 1 cut(s) 282
Csp6I GTAC 2 cut(s) 364, 1172
CviAII CATG 7 cut(s) 42, 46, 212, 217, 817, 1009, 1164
CviQI GTAC 2 cut(s) 364, 1172
DdeI CTNAG 3 cut(s) 580, 938, 1185
DpnI GATC 5 cut(s) 312, 354, 492, 1041, 1134
DpnII GATC 5 cut(s) 310, 352, 490, 1039, 1132
Eam1104I CTCTTC 1 cut(s) 795
EarI CTCTTC 1 cut(s) 795
Eco130I CCWWGG 3 cut(s) 216, 816, 1008
Eco47I GGWCC 1 cut(s) 282
Eco57I CTGAAG 2 cut(s) 529, 1020
EcoNI CCTNNNNNAGG 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 56, 646, 731
EcoT14I CCWWGG 3 cut(s) 216, 816, 1008
EcoT22I ATGCAT 2 cut(s) 47, 749
ErhI CCWWGG 3 cut(s) 216, 816, 1008
Esp3I CGTCTC 1 cut(s) 1213
FaeI CATG 7 cut(s) 45, 49, 215, 220, 820, 1012, 1167
FalI AAGNNNNNCTT 2 cut(s) 1018, 1050
FaqI GGGAC 2 cut(s) 97, 277
FatI CATG 7 cut(s) 41, 45, 211, 216, 816, 1008, 1163
FbaI TGATCA 1 cut(s) 490
FblI GTMKAC 2 cut(s) 13, 165
Fnu4HI GCNGC 3 cut(s) 507, 635, 768
FokI GGATG 1 cut(s) 834
Fsp4HI GCNGC 3 cut(s) 507, 635, 768
FspBI CTAG 1 cut(s) 794
GlaI GCGC 1 cut(s) 108
GluI GCNGC 3 cut(s) 507, 635, 768
HapII CCGG 2 cut(s) 329, 810
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 1206
Hin1II CATG 7 cut(s) 45, 49, 215, 220, 820, 1012, 1167
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HinfI GANTC 9 cut(s) 272, 344, 530, 663, 920, 934, 975, 980, 1004
HpaII CCGG 2 cut(s) 329, 810
Hpy166II GTNNAC 4 cut(s) 14, 166, 475, 697
Hpy188I TCNGA 6 cut(s) 25, 529, 548, 607, 941, 1039
Hpy188III TCNNGA 2 cut(s) 356, 1150
Hpy8I GTNNAC 4 cut(s) 14, 166, 475, 697
HpyAV CCTTC 3 cut(s) 22, 534, 874
HpyCH4III ACNGT 4 cut(s) 163, 485, 618, 763
HpyCH4IV ACGT 2 cut(s) 1159, 1206
HpyF10VI GCNNNNNNNGC 3 cut(s) 512, 709, 773
HpyF3I CTNAG 3 cut(s) 580, 938, 1185
HpySE526I ACGT 2 cut(s) 1159, 1206
Hsp92I GRCGYC 1 cut(s) 1206
Hsp92II CATG 7 cut(s) 45, 49, 215, 220, 820, 1012, 1167
HspAI GCGC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 5 cut(s) 310, 352, 490, 1039, 1132
LmnI GCTCC 2 cut(s) 677, 812
Lsp1109I GCAGC 3 cut(s) 493, 621, 779
LweI GCATC 1 cut(s) 187
MaeI CTAG 1 cut(s) 794
MaeII ACGT 2 cut(s) 1159, 1206
MalI GATC 5 cut(s) 312, 354, 492, 1041, 1134
MboI GATC 5 cut(s) 310, 352, 490, 1039, 1132
MboII GAAGA 3 cut(s) 124, 233, 782
MflI RGATCY 2 cut(s) 1039, 1132
MluCI AATT 7 cut(s) 187, 205, 257, 558, 639, 874, 1103
MlyI GAGTC 1 cut(s) 989
MmeI TCCRAC 1 cut(s) 1055
MnlI CCTC 6 cut(s) 126, 245, 698, 764, 798, 1191
Mph1103I ATGCAT 2 cut(s) 47, 749
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 4 cut(s) 74, 324, 395, 1086
MslI CAYNNNNRTG 2 cut(s) 50, 501
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 2 cut(s) 329, 810
MspR9I CCNGG 4 cut(s) 58, 330, 648, 733
Mva1269I GAATGC 1 cut(s) 870
MvaI CCWGG 3 cut(s) 58, 648, 733
MwoI GCNNNNNNNGC 3 cut(s) 512, 709, 773
NciI CCSGG 1 cut(s) 330
NcoI CCATGG 3 cut(s) 216, 816, 1008
NdeII GATC 5 cut(s) 310, 352, 490, 1039, 1132
NlaIII CATG 7 cut(s) 45, 49, 215, 220, 820, 1012, 1167
NlaIV GGNNCC 6 cut(s) 39, 68, 334, 737, 814, 1014
NsiI ATGCAT 2 cut(s) 47, 749
NspI RCATGY 1 cut(s) 1167
NspV TTCGAA 1 cut(s) 878
PaeI GCATGC 1 cut(s) 1167
PaqCI CACCTGC 1 cut(s) 1147
PctI GAATGC 1 cut(s) 870
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 8 cut(s) 272, 344, 530, 663, 920, 934, 975, 1004
PflMI CCANNNNNTGG 1 cut(s) 63
PfoI TCCNGGA 1 cut(s) 731
PkrI GCNGC 3 cut(s) 508, 636, 769
PleI GAGTC 1 cut(s) 988
PpsI GAGTC 1 cut(s) 988
Ppu21I YACGTR 1 cut(s) 1160
Psp6I CCWGG 3 cut(s) 56, 646, 731
PspGI CCWGG 3 cut(s) 56, 646, 731
PspN4I GGNNCC 6 cut(s) 39, 68, 334, 737, 814, 1014
PspPI GGNCC 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 2 cut(s) 1039, 1132
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 2 cut(s) 365, 1173
RsaNI GTAC 2 cut(s) 364, 1172
RseI CAYNNNNRTG 2 cut(s) 50, 501
SaqAI TTAA 4 cut(s) 74, 324, 395, 1086
SatI GCNGC 3 cut(s) 507, 635, 768
Sau3AI GATC 5 cut(s) 310, 352, 490, 1039, 1132
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 1 cut(s) 989
ScrFI CCNGG 4 cut(s) 58, 330, 648, 733
SfaNI GCATC 1 cut(s) 187
SfuI TTCGAA 1 cut(s) 878
SinI GGWCC 1 cut(s) 282
SmiMI CAYNNNNRTG 2 cut(s) 50, 501
SphI GCATGC 1 cut(s) 1167
Sse9I AATT 7 cut(s) 187, 205, 257, 558, 639, 874, 1103
SsiI CCGC 3 cut(s) 136, 141, 249
SspMI CTAG 1 cut(s) 794
StyD4I CCNGG 4 cut(s) 56, 328, 646, 731
StyI CCWWGG 3 cut(s) 216, 816, 1008
TaaI ACNGT 4 cut(s) 163, 485, 618, 763
TaiI ACGT 2 cut(s) 1162, 1209
TaqI TCGA 6 cut(s) 313, 625, 836, 870, 878, 978
TasI AATT 7 cut(s) 187, 205, 257, 558, 639, 874, 1103
TfiI GAWTC 8 cut(s) 272, 344, 530, 663, 920, 934, 975, 1004
Tru1I TTAA 4 cut(s) 74, 324, 395, 1086
Tru9I TTAA 4 cut(s) 74, 324, 395, 1086
TscAI CASTG 2 cut(s) 343, 355
TseI GCWGC 3 cut(s) 506, 634, 767
TspDTI ATGAA 6 cut(s) 200, 413, 816, 854, 933, 1115
TspGWI ACGGA 1 cut(s) 987
TspRI CASTG 2 cut(s) 343, 355
Van91I CCANNNNNTGG 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 792
XapI RAATTY 1 cut(s) 1103
XceI RCATGY 1 cut(s) 1167
XmiI GTMKAC 2 cut(s) 13, 165
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 1 cut(s) 794
ZraI GACGTC 1 cut(s) 1207
Zsp2I ATGCAT 2 cut(s) 47, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.